CN112293433A - Environment-friendly enzyme mosquito repellent liquid and preparation method thereof - Google Patents
Environment-friendly enzyme mosquito repellent liquid and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/08—Magnoliopsida [dicotyledons]
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
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Abstract
The invention relates to an environment-friendly enzyme mosquito repellent liquid and a preparation method thereof. The mosquito repellent liquid is prepared from raw materials, decomposition and activation auxiliary materials and slow-release nutrients; the raw materials comprise, by weight, 12-15 parts of citrus leaves, 12-15 parts of citrus peels, 3-5 parts of garlic cloves, 3-5 parts of ageratum leaves, 2-3 parts of clove leaves and 1-2 parts of folium artemisiae argyi; decomposing the activated adjunct may utilize the slow release nutrient to grow and decompose the raw material to produce the enzyme. The invention utilizes common plant materials beneficial to human bodies and uses grains as a fermentation catalyst, so that the source is wide, the cost is low, and the invention is green and environment-friendly; the mosquito repellent liquid provides ferment volatile matters for human bodies, improves the sleep of users, plays a role in health preservation and health care, and is suitable for children and special pregnant women.
Description
Technical Field
The invention relates to the technical field of mosquito repellent liquid, and particularly relates to environment-friendly enzyme mosquito repellent liquid and a preparation method thereof.
Background
Various mosquito repellent products on the market achieve the mosquito repellent effect by emitting various gases or smoke of mosquito repellent incense in the air, people can inhale the gases or the smoke while repelling mosquitoes, bad reaction is brought to the body, mobility is not good, and people can not be protected once leaving the region. And because products such as mosquito repellent water on the market belong to inflammables and contain mosquito repellent amine and other toxic components, long-term use is not recommended.
Disclosure of Invention
In view of the above, there is a need to provide an environment-friendly enzyme mosquito repellent liquid, which is used to solve the problem that the existing mosquito repellent product has a great side effect on human body.
The invention provides an environment-friendly enzyme mosquito repellent liquid which is prepared from raw materials, decomposition and activation auxiliary materials and slow-release nutrients; the raw materials comprise, by weight, 12-15 parts of citrus leaves, 12-15 parts of citrus peels, 3-5 parts of garlic cloves, 3-5 parts of ageratum leaves, 2-3 parts of clove leaves and 1-2 parts of folium artemisiae argyi; the decomposition activating adjuvant may grow using the slow release nutrient and decompose the raw material to produce an enzyme.
The invention also provides a preparation method of the environment-friendly ferment mosquito repellent liquid as claimed in claim 1, which comprises the following steps:
freezing each component of the raw material respectively, and grinding the components into each component particle respectively;
the preparation method comprises the steps of mixing and grouping the particles of the components in groups, adding one sustained-release nutrient into each group, implanting one decomposition and activation auxiliary material into each group, fermenting at 35-40 ℃ and under the humidity range of 60-80%, spraying water during the fermentation process to obtain each fermented product, and leaching and mixing the fermented products to obtain the environment-friendly mosquito repellent liquid.
Specifically, the component particles comprise citrus peel particles, clove leaf particles, tangerine leaf particles, ageratum leaves particles, garlic clove particles and folium artemisiae argyi particles;
uniformly mixing the citrus peel particles and the clove leaf particles to obtain first component particles; uniformly mixing the tangerine leaf particles and the agastache leaf particles to obtain second component particles; mixing Bulbus Allii micro-particles and folium Artemisiae Argyi micro-particles uniformly to obtain third component granule.
Specifically, the decomposition and activation auxiliary material comprises at least one of a high-temperature bending monospore bacterial agent, a corn smut bacterial agent, an aerobic sliding slime bacterial agent and a candida arborescens bacterial agent.
Specifically, the slow-release nutrient comprises 3-5 parts of soybean cake powder and 5-8 parts of corn starch in parts by weight.
Specifically, the fermentation comprises the following steps:
mixing the first component particles, the slow-release nutrient, the high-temperature bending monascus microbial inoculum and the corn smut microbial inoculum for first fermentation to obtain a first fermentation product;
mixing the second component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum and the aerobic sliding slime microbial inoculum for second fermentation to obtain a second fermentation product;
mixing the third component particles, the slow-release nutrient, the high-temperature bending monospore microbial inoculum, the corn smut microbial inoculum and the aerobic sliding slime microbial inoculum for third fermentation to obtain a third fermentation product;
and mixing the slow-release nutrient with the candida arborescens microbial inoculum for fourth fermentation to obtain a fourth fermentation product.
Specifically, the fermentation time is 2-3 days;
in the first fermentation process, the weight ratio of the first component particles, the slow-release nutrient, the high-temperature bending monascus microbial inoculum, the corn smut microbial inoculum and water is (3-5): (30-60): (1-2): 100;
in the second fermentation process, the weight ratio of the second component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum, the aerobic sliding slime microbial inoculum and water is (2-4): (30-60): 1-2): 0.5-1): 100;
in the third fermentation process, the weight ratio of the third component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum, the corn black powder microbial inoculum, the aerobic sliding slime microbial inoculum and water is (2-4), (30-60), (1-2), (0.5-1) and (100);
in the fourth fermentation process, the weight ratio of the slow-release nutrient to the candida arborescens microbial inoculum to the water is (30-60): 1-2): 100.
Specifically, the first ferment is extracted by a solvent which is mixed and dissolved by ethyl acetate and absolute ethyl alcohol according to the volume ratio of 1: 1; the second fermentation product is extracted by using ethyl acetate as a solvent; the third fermentation product is extracted by adopting a solvent which is miscible with water and ethanol according to the volume ratio of 5: 1; the fourth fermentation product is extracted by using ethyl acetate as a solvent.
Has the advantages that:
the invention utilizes common plant materials beneficial to human bodies and uses grains as a fermentation catalyst, so that the source is wide, the cost is low, and the invention is green and environment-friendly; the mosquito repellent provides ferment volatile matters for human bodies, improves the sleep of users, and has health preserving and health care effects.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The embodiment of the invention provides an environment-friendly enzyme mosquito repellent liquid which is prepared from raw materials, decomposition and activation auxiliary materials and slow-release nutrients;
the raw materials comprise, by weight, 12-15 parts of citrus leaves, 12-15 parts of citrus peels, 3-5 parts of garlic cloves, 3-5 parts of ageratum leaves, 2-3 parts of clove leaves and 1-2 parts of folium artemisiae argyi;
the decomposition activating adjuvant may grow using the slow release nutrient and decompose the raw material to produce an enzyme.
In more embodiments, the present invention further provides a preparation method of the above environment-friendly ferment mosquito repellent liquid, including the following steps:
freezing each component of the raw material respectively, and grinding the components into each component particle respectively;
the preparation method comprises the steps of mixing and grouping the particles of the components in groups, adding one sustained-release nutrient into each group, implanting one decomposition and activation auxiliary material into each group, fermenting at 35-40 ℃ and under the humidity range of 60-80%, spraying water during the fermentation process to obtain each fermented product, and leaching and mixing the fermented products to obtain the environment-friendly mosquito repellent liquid.
The freezing process of the raw materials comprises the following specific steps:
1) each component of the raw material is respectively contained by a polytetrafluoroethylene net and is identified to obtain a classified raw material to be used;
2) preparing absolute ethyl alcohol, filling the absolute ethyl alcohol into a nitrile rubber barrel, continuously infusing dry ice into the nitrile rubber barrel until the temperature of the absolute ethyl alcohol is reduced to-50 ℃, controlling the infusion speed, and keeping the temperature within the range of-70 ℃ to-50 ℃ to obtain an ice barrel to be used;
3) respectively and simultaneously immersing the classified raw materials to be used into the ice buckets to be used obtained in the step 2), and taking out after 3-5 min to obtain classified frozen raw materials;
4) grinding the raw material components obtained by classification and freezing in the step 3) into particles with the particle size of 0.1-0.3 mm by a mechanical grinding device to obtain tangerine leaf particles, citrus peel particles, garlic clove particles, ageratum leaves particles, clove leaf particles and folium artemisiae argyi particles.
The freezing process of the raw materials is taken as an example, and the raw materials are directly ground without freezing or ground after drying respectively as a comparative example.
In addition, the raw material components were also grouped as examples. Grouping is as follows: uniformly mixing the citrus peel particles and the clove leaf particles to obtain first component particles; uniformly mixing the tangerine leaf particles and the agastache leaf particles to obtain second component particles; mixing Bulbus Allii micro-particles and folium Artemisiae Argyi micro-particles uniformly to obtain third component granule.
In addition, the above-mentioned component particles were not directly mixed or grouped according to the above principle as comparative examples, and the raw material components provided for the use of the present invention were used as comparative examples.
In a specific embodiment, the decomposition and activation auxiliary material comprises at least one of a high-temperature single spore bacterium agent, a corn smut bacterium agent, an aerobic slide slime bacterium agent and a candida arborescens bacterium agent. Some common microbial inoculum which is not provided by the invention is taken as a comparative example, such as lactobacillus plantarum.
In a specific embodiment, the slow-release nutrient comprises 3-5 parts by weight of soybean cake powder and 5-8 parts by weight of corn starch. Some common slow release nutrients not provided by the present invention are used as comparative examples, such as soy shares or flour.
A fermentation mode:
mixing the first component particles, the slow-release nutrient, the high-temperature bending monascus microbial inoculum and the corn smut microbial inoculum for first fermentation to obtain a first fermentation product;
mixing the second component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum and the aerobic sliding slime microbial inoculum for second fermentation to obtain a second fermentation product;
mixing the third component particles, the slow-release nutrient, the high-temperature bending monospore microbial inoculum, the corn smut microbial inoculum and the aerobic sliding slime microbial inoculum for third fermentation to obtain a third fermentation product;
and mixing the slow-release nutrient with the candida arborescens microbial inoculum for fourth fermentation to obtain a fourth fermentation product.
This fermentation was conducted as an example, and the fermentation without this fermentation in a divided manner was conducted as a comparative example.
Specifically, the fermentation time is 2-3 days.
Examples of fermentations carried out in the above manner:
in the first fermentation process, the weight ratio of the first component particles, the slow-release nutrient, the high-temperature bending monospore bacterial agent, the corn smut bacterial agent and water is (3-5): (30-60): (1-2): 100;
in the second fermentation process, the weight ratio of the second component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum, the aerobic sliding slime microbial inoculum and water is (2-4): (30-60): (1-2): 0.5-1): 100;
in the third fermentation process, the weight ratio of the third component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum, the corn black powder microbial inoculum, the aerobic sliding slime microbial inoculum and water is (2-4), (30-60), (1-2), (0.5-1) and (100);
in the fourth fermentation process, the weight ratio of the slow-release nutrient to the candida arborescens microbial inoculum to the water is (30-60): 1-2): 100.
Leaching of fermentation products: the first ferment is extracted by a solvent which is mixed and dissolved by ethyl acetate and absolute ethyl alcohol according to the volume ratio of 1: 1; the second fermentation product is extracted by using ethyl acetate as a solvent; the third fermentation product is extracted by a solvent which is miscible with water and ethanol according to the volume ratio of 5: 1; the fourth fermentation product is extracted by using ethyl acetate as a solvent. All fermentations were leached with ethyl acetate as comparative examples.
More specific examples of the preparation process of the environment-friendly ferment mosquito repellent liquid provided by the invention are shown in table 1. Table 1 considers the treatment of the raw material (marked a), the grouping of the component granules (marked B), the selection of the decomposition-activation adjuvant (marked C), the selection of the slow-release nutrient (marked D), the selection of the fermentation mode (marked E), the proportioning of the raw materials in the fermentation process (marked F) and the extraction process (marked G).
The processing mode of the raw materials comprises the following steps: a1 freezing according to the freezing process provided in the above examples, A2 grinding the raw material components directly without freezing, A3 drying the raw material and grinding; the raw material components which do not contain orange peel, tangerine leaves, agastache leaves and garlic cloves are used as A4, and the raw materials which do not accord with the invention are used as A5 according to the weight ratio.
Wherein, the grouping mode of the component particles is as follows: b1 is divided into three groups in the manner provided in the above embodiments; b2 directly mixing the component particles; b3 is divided into particles with citrus peel as the first component; uniformly mixing clove leaf particles, tangerine leaf particles and ageratum leaf particles to obtain second component particles; uniformly mixing the garlic clove particles and the folium artemisiae argyi particles to obtain third component particles; b4 is divided into first component particles which are formed by evenly mixing citrus peel particles, clove leaf particles and tangerine leaf particles; taking the fine particles of the agastache leaves as second component particles; mixing Bulbus Allii micro-particles and folium Artemisiae Argyi micro-particles uniformly to obtain third component granule.
Wherein, the decomposition activating auxiliary material provided by the invention is selected to be C1, and the lactobacillus plantarum is selected to be C2. The slow-release nutrition provided by the invention is D1, and the soybean part is D2.
Wherein, the fermentation mode is divided into: e1 is the grouping fermentation mode provided by the invention, E2 is the grouping fermentation mode provided by the invention after the B2 component particles are divided into four parts; e3 is that the component particles grouped by the B3 are fermented in a grouping fermentation mode corresponding to the grouping fermentation mode provided by the invention (for example, the first component particles are citrus peel particles, and the first fermentation is carried out); e4 is that the component particles grouped by the B3 grouping mode are fermented in a grouping fermentation mode corresponding to the grouping fermentation mode provided by the invention (for example, the first component particles are a mixture of citrus peel particles, clove leaf particles and citrus leaf particles, and the first fermentation is carried out).
In the embodiment F1, the fermentation raw materials of the first fermentation, the second fermentation, the third fermentation and the fourth fermentation are fermented according to the mass ratio provided by the present invention, and the embodiment in which the fermentation is not performed according to the mass ratio is F2.
Wherein, the leaching process comprises the following steps: the leaching mode provided by the invention is G1, and ethyl acetate is used for leaching to obtain G2.
TABLE 1
Evaluation of mosquito repellent liquid
Test animals: culex fatigues is adopted, blood is not absorbed 4-5 days after emergence, and adult mosquitoes are female. The weight of a white mouse is 20-25 g, the weight of an SD rat is 180-200 g, and the weight of a big-ear rabbit is 2-2.5 kg.
The mosquito repellent effect test of the white mouse:
300 Culex fatigues vitamin are respectively put into 5 mosquito cages of 30mm multiplied by 30 mn. The abdomen of 5 mice was fixed upward, and the skin of the abdomen Mao Gong Lu was cut off. 0.063ml/cm on 2h, 4h and 8h of the day of experiment start2The ant repelling liquid provided by the above examples and comparative examples was evenly spread on the 20mm x 30mm area of skin obstinate exposed part of the abdomen of 4 mice in the experimental group to be tested. Another 1 mouse served as a blank control. The remainder of the body of the mice was covered tightly with white cloth. And (5) putting S mice to be tested into a mosquito cage at the same time at 17:00 night under the condition of dusk witness, and taking out the mice after 5 nin. Then, taking out all mosquitoes in the mosquito cage by using a mosquito sucking path, and recording the number of blood-sucking mosquitoes after anesthesia. The blank control blood sucking mosquito number is more than 30 plants, and the attack power of the ant is qualified. The experimental group judges that the mosquito repelling fails as long as 1 mosquito sucks blood. The effective protection time of the mosquito repellent water to people is deduced by replacing hands with mice. The temperature of the real riding chamber is 26-27 ℃, and the relative humidity is 60-70%.
Acute skin irritation test: selecting 4 healthy white rabbits with big ears, and half of the rabbits in male and female; in the day before the experiment, the hair on both sides of the spine of the home-use department is cut off, and the hair removing range is about 30mm multiplied by 30 mn. In the experiment, 0.2ml of the mosquito repellent liquid provided by the above examples and comparative examples is uniformly dropped on two layers of gauze with the thickness of 25mm multiplied by 25mm, attached to the skin of the left unhairing area, covered by a layer of cellophane, and fixed by a mask. The application time is 4h, and the skin of the right unhairing area is used as a white control. The local skin reactions were observed at 1h, 24h, and 48h after removal of the test substance.
Acute eye irritation test: selecting 4 healthy white rabbits with big ears, carving male half of the rabbits, checking the normality of the two eyes of the rabbits before the experiment, taking 0.1ml of sample during the experiment, dripping the sample into the conjunctival sac of the left eyes of the rabbits, passively closing the eyes for 4s, and flushing the eyes for 5min by using isotonic saline; the right eye was treated as a control eye by the same method except that 0.1ml of distilled water was added, and local responses of 1 hour (6 minutes for the presence of the stimulus), 24 hours (3 minutes for the presence of the stimulus), 48 hours (1.5 minutes for the presence of the stimulus), 72 hours (1.0 minute for the presence of the stimulus), 4d (0.5 minutes for the presence of the stimulus), and 7d (0 minutes for the presence of the stimulus) after the administration were recorded, and graded integration and accumulation of ocular lesions were carried out, and the higher the integration, the greater the irritation.
The enzyme content in the above examples and comparative examples is determined with reference to GB/T12456-2008.
TABLE 2 mosquito repellent results in mice
| Examples | Number of mosquitoes sucking blood for 2h | 4h blood sucking mosquito number | Number of blood sucking mosquito within 8h |
| Example 1 | 0 | 0 | 4 |
| Comparative example 1 | 2 | 3 | 10 |
| Comparative example 2 | 2 | 4 | 12 |
| Comparative example 3 | 2 | 4 | 13 |
| Comparative example 4 | 2 | 5 | 13 |
| Comparative example 5 | 2 | 5 | 11 |
| Comparative example 6 | 3 | 7 | 15 |
| Comparative example 7 | 4 | 7 | 12 |
| Comparative example 8 | 2 | 8 | 16 |
| Comparative example 9 | 3 | 9 | 20 |
| Comparative example 10 | 4 | 10 | 21 |
| Comparative example 11 | 3 | 11 | 19 |
TABLE 3 acute skin irritation results
TABLE 4 eye irritation test results
| Examples | Integral index of eye | 1h | 24h | 48h | 72h | 4d | 7d |
| Example 1 | 1.5 | 0 | 0 | 0 | 1.0 | 0.5 | 0 |
| Comparative example 1 | 3 | 0 | 0 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 2 | 6 | 0 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 3 | 6 | 0 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 4 | 6 | 0 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 5 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 6 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 7 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 8 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 9 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 10 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
| Comparative example 11 | 12 | 6 | 3 | 1.5 | 1.0 | 0.5 | 0 |
TABLE 5 enzyme content
| Examples | Total acid content of ferment (wt ‰) |
| Example 1 | 0.22 |
| Comparative example 1 | 0.13 |
| Comparative example 2 | 0.15 |
| Comparative example 3 | 0.12 |
| Comparative example 4 | 0.062 |
| Comparative example 5 | 0.051 |
| Comparative example 6 | 0.044 |
| Comparative example 7 | 0.035 |
| Comparative example 8 | 0.037 |
| Comparative example 9 | 0.026 |
| Comparative example 10 | 0.023 |
| Comparative example 11 | 0.027 |
As can be seen from tables 2 to 5: according to the preparation method of the mosquito repellent liquid, common plant raw materials are selected, freezing and grinding are carried out, grouping fermentation is carried out, different decomposition and activation auxiliary materials and slow-release nutrients are adopted, so that a good repelling effect and a good protection time are remarkably improved, meanwhile, the protection time is longer than 6 hours, the toxicity is extremely low, and the irritation to skin and eyes is extremely low; the mosquito repellent liquid provided by the invention contains a certain amount of enzyme acid, so that the health-care function and the aromatic smell of the mosquito repellent liquid can be improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (8)
1. An environment-friendly enzyme mosquito repellent liquid is characterized by being prepared from raw materials, decomposition and activation auxiliary materials and slow-release nutrients;
the raw materials comprise, by weight, 12-15 parts of citrus leaves, 12-15 parts of citrus peels, 3-5 parts of garlic cloves, 3-5 parts of ageratum leaves, 2-3 parts of clove leaves and 1-2 parts of folium artemisiae argyi;
the decomposition activating adjuvant may grow using the slow release nutrient and decompose the raw material to produce an enzyme.
2. The preparation method of the environment-friendly enzyme mosquito repellent liquid as claimed in claim 1, which is characterized by comprising the following steps of:
freezing each component of the raw material respectively, and grinding the components into each component particle respectively;
the preparation method comprises the steps of mixing and grouping the particles of the components in groups, adding one sustained-release nutrient into each group, implanting one decomposition and activation auxiliary material into each group, fermenting at 35-40 ℃ and under the humidity range of 60-80%, spraying water during the fermentation process to obtain each fermented product, and leaching and mixing the fermented products to obtain the environment-friendly enzyme mosquito repellent liquid.
3. The method of claim 2, wherein the component particles comprise citrus peel particles, clove leaf particles, citrus leaf particles, ageratum leaf particles, garlic clove leaf particles, artemisia argyi particles;
uniformly mixing the citrus peel particles and the clove leaf particles to obtain first component particles; uniformly mixing the tangerine leaf particles and the agastache leaf particles to obtain second component particles; mixing Bulbus Allii micro-particles and folium Artemisiae Argyi micro-particles uniformly to obtain third component granule.
4. The preparation method according to claim 3, wherein the decomposition activation auxiliary material comprises at least one of a Thermomonospora flexuosus bacterial agent, a Ustilago maydis bacterial agent, an aerobic Gliocladium sliddium bacterial agent, and a Candida arborescens bacterial agent.
5. The preparation method according to claim 4, wherein the slow-release nutrient comprises 3-5 parts by weight of soybean cake meal and 5-8 parts by weight of corn starch.
6. The method of claim 5, wherein the fermenting comprises:
mixing the first component particles, the slow-release nutrient, the high-temperature bending monascus microbial inoculum and the corn smut microbial inoculum for first fermentation to obtain a first fermentation product;
mixing the second component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum and the aerobic sliding slime microbial inoculum for second fermentation to obtain a second fermentation product;
mixing the third component particles, the slow-release nutrient, the high-temperature bending monospore microbial inoculum, the corn smut microbial inoculum and the aerobic sliding slime microbial inoculum for third fermentation to obtain a third fermentation product;
and mixing the slow-release nutrient with the candida arborescens microbial inoculum for fourth fermentation to obtain a fourth fermentation product.
7. The method according to claim 6, wherein the fermentation time is 2 to 3 days;
in the first fermentation process, the weight ratio of the first component particles, the slow-release nutrient, the high-temperature bending monascus microbial inoculum, the corn smut microbial inoculum and water is (3-5): (30-60): (1-2): 100;
in the second fermentation process, the weight ratio of the second component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum, the aerobic sliding slime microbial inoculum and water is (2-4): (30-60): 1-2): 0.5-1): 100;
in the third fermentation process, the weight ratio of the third component particles, the slow-release nutrient, the bent high-temperature monospore microbial inoculum, the corn black powder microbial inoculum, the aerobic sliding slime microbial inoculum and water is (2-4), (30-60), (1-2), (0.5-1) and (100);
in the fourth fermentation process, the weight ratio of the slow-release nutrient to the candida arborescens microbial inoculum to the water is (30-60): 1-2): 100.
8. The preparation method according to claim 6, wherein the first fermentation product is extracted by using a solvent in which ethyl acetate and absolute ethyl alcohol are miscible in a volume ratio of 1: 1; the second fermentation product is extracted by using ethyl acetate as a solvent; the third fermentation product is extracted by adopting a solvent which is miscible with water and ethanol according to the volume ratio of 5: 1; the fourth fermentation product is extracted by using ethyl acetate as a solvent.
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