CN109329323A - A kind of draft ferment insecticide and its preparation method and application - Google Patents
A kind of draft ferment insecticide and its preparation method and application Download PDFInfo
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- CN109329323A CN109329323A CN201811282980.3A CN201811282980A CN109329323A CN 109329323 A CN109329323 A CN 109329323A CN 201811282980 A CN201811282980 A CN 201811282980A CN 109329323 A CN109329323 A CN 109329323A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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Abstract
The invention discloses a kind of draft ferment insecticides and its preparation method and application, the active constituent of the draft ferment insecticide is fermented by the extracted rear inoculation compound bacteria of draft Chinese medicine, the draft Chinese medicine includes kuh-seng, the tuber of stemona, Fructus meliae toosendan and the fruit of Rangoon creeper, and the compound bacteria includes Candida boidinii, lactobacillus buchneri and Senegal's acetic acid bacteria；The preparation method includes: to decoct draft Chinese medicine to obtain Chinese medical extract, and carbohydrate is added and deploys the Chinese traditional medicine dispensing liquid that sterilizes to obtain, inoculation compound bacteria fermentation is filtered, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide after inactivation.The present invention passes through the reasonable compatibility to draft Chinese medicine, using compound bacteria co-fermentation, predigestion, decomposition and conversion are carried out to draft Chinese medicine, the intermediate material of macromolecular is decomposed into the effective small-molecule substance that can be directly absorbed, the absorption that can accelerate draft Chinese medicine, significantly effectively improves insecticidal effect；Preparation method is simple, and technique is controllable, and actual promotional value is important.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to draft ferment in the control and application technical field of diseases and pests of agronomic crop,
More particularly to a kind of draft ferment insecticide and its preparation method and application.
Background technique
Insecticide is primarily referred to as the drug of prevention and treatment agricultural, forestry pest and urban health insect.The use of insecticide is gone through
History is remote, various in style, and dosage is huge.Since twentieth century, with the rapid development of China's agricultural, diseases and pests of agronomic crop
As one of China's agricultural major casualty, and insecticide uses so that farm output is sharply increased, therefore, insecticide gradually at
For one of product essential in agricultural production process, and dosage presents a rapidly rising trend.
Largely also increasingly shown using harm caused by chemical insecticide, specifically included, agriculture production cost is high, drug effect
Time is short, and drug resistance is strong, and residual range is wide etc.；In addition, also the moment jeopardizes the life security of human and animal to chemical insecticide, entirely
State's various regions insecticide poisoning event happens occasionally.
With the food-safe consciousness of people enhancing and Environmental security there is an urgent need to, the whole society especially agriculture, woods,
The animal husbandry producer is badly in need of a kind of efficient, low toxicity and environmental-friendly insecticide.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides one kind is made using natural Chinese medicinal herb as raw material, through strain fermentation
At have good disinsection effect, low toxicity, non-harmful draft ferment insecticide.
It is a further object of the present invention to provide the draft ferment method for producing insecticide.
It is yet another object of the invention to provide the applications of the draft ferment insecticide.
The purpose of the present invention is achieved through the following technical solutions:
A kind of draft ferment insecticide, active constituent are fermented by the extracted rear inoculation compound bacteria of draft Chinese medicine.
The draft Chinese medicine includes: kuh-seng, the tuber of stemona, Fructus meliae toosendan and the fruit of Rangoon creeper.
The compound bacteria includes: Candida boidinii, lactobacillus buchneri and Senegal's acetic acid bacteria.
Wherein, kuh-seng, it is bitter, it trembles with fear, return heart, liver, stomach, large intestine, bladder meridian, heat-clearing and damp-drying drug, diuresis, desinsection, calming heart, which stops, throbs with fear, and uses
In damp-heat dysentery, hematochezia, jaundice and reddish urine, the puckery pain of stranguria, difficult urination, leukorrhea with reddish discharge, swelling of vulva pruritus vulvae, eczema wet sore, skin scabies
Itch, castor, palpitation and uneasiness.
Wherein, the tuber of stemona, sweet in flavor, bitter, slightly warm in nature is distributed in lung channel, moistening lung to lower qi cough-relieving, and desinsection and delousion is coughed for new chronic cough, lung
Consumptive disease cough, pertussis are used for head louse, body louse, enterobiasis, pruritus vulvae, sweet tuber of stemona moistening lung to arrest cough, for deficiency of yin and overstrain cough outside.
Wherein, Fructus meliae toosendan, it is bitter, it trembles with fear, returns liver, small intestine, bladder meridian is soothing the liver to expel the heat-evil, promoting qi circulation and relieving pain, desinsection, is used for liver depression
Fire, chest side of body, abdominal distention, hernia pain, abdominal pain due to enterositosis.
Wherein, the fruit of Rangoon creeper, sweet, warm, returns spleen, stomach meridian, killing insect and eliminating accumulation are used for roundworm disease, enterobiasis, abdominal pain due to enterositosis, children
Infantile malnutrition due to digestive disturbances or intestinalparasites.
In the above-mentioned technical solutions, the draft Chinese medicine is made of the raw material of following parts by weight:
80-150 parts of kuh-seng, 80-150 parts of the tuber of stemona, 50-100 parts of Fructus meliae toosendan and 50-100 parts of the fruit of Rangoon creeper.
In the above-mentioned technical solutions, in the compound bacteria, Candida boidinii, lactobacillus buchneri and Senegal's vinegar
The viable count ratio of sour bacterium is (5-7.5): (1-1.5): (1-1.5).
Preferably, in the above-mentioned technical solutions, the compound bacteria is provided in the form of microbial inoculum, by containing viable count 5 ×
107-7.5×1070.8-1.2 parts of the Candida boidinii microbial inoculum of cfu/g contains viable count 1.0 × 107-1.25×
1070.8-1.2 parts of the lactobacillus buchneri microbial inoculum of cfu/g and contain viable count 1.0 × 107-1.25×107The Senegal of cfu/g
0.8-1.2 parts of acetic acid bacteria agent compositions.
In the above-mentioned technical solutions, the additional amount of the composite bacteria agent is the 0.1-1wt% of the draft Chinese medicine total amount.
Further, in the above-mentioned technical solutions, the draft Chinese medicine is made of the raw material of following parts by weight:
96-135 parts of kuh-seng, 112-142 parts of the tuber of stemona, 65-88 parts of Fructus meliae toosendan and 58-95 parts of the fruit of Rangoon creeper；
The compound bacteria is provided in the form of microbial inoculum, by containing viable count 5 × 107-7.5×107The Bo Yiding of cfu/g is false
0.89-1.08 parts of yeast agent of silk contains viable count 1.0 × 107-1.25×107The lactobacillus buchneri microbial inoculum 0.83- of cfu/g
1.02 parts and contain viable count 1.0 × 107-1.25×1070.94-1.15 parts of the Senegal's acetic acid bacteria agent compositions of cfu/g,
The additional amount of the composite bacteria agent is the 0.4-0.9wt% of the draft Chinese medicine total amount.
Still further, in the above-mentioned technical solutions, the draft Chinese medicine is made of the raw material of following parts by weight:
102-128 parts of kuh-seng, 118-135 parts of the tuber of stemona, 68-82 parts of Fructus meliae toosendan and 62-88 parts of the fruit of Rangoon creeper；
The compound bacteria is provided in the form of microbial inoculum, by containing viable count 5 × 107-7.5×107The Bo Yiding of cfu/g is false
0.92-1.05 parts of yeast agent of silk contains viable count 1.0 × 107-1.25×107The lactobacillus buchneri microbial inoculum 0.87- of cfu/g
0.98 part and contain viable count 1.0 × 107-1.25×1070.97-1.09 parts of the Senegal's acetic acid bacteria agent compositions of cfu/g,
The additional amount of the composite bacteria agent is the 0.48-0.78wt% of the draft Chinese medicine total amount.
Further, in the above-mentioned technical solutions,
The deposit number of the Candida boidinii is CCTCC M 2018162BST003；
The deposit number of the lactobacillus buchneri is CCTCC M 2018164BST005；
The deposit number of Senegal's acetic acid bacteria is CCTCC M 2018167BST008.
The present invention provides the draft ferment method for producing insecticide, include the following steps:
S1, draft Chinese medicine is weighed according to the ratio, the decoction liquor merged twice twice is added water to cook after immersion and obtains traditional Chinese medicine extraction
Object；
S2, carbohydrate, mixing preparation are added into the Chinese medical extract, high-temperature sterilization obtains Chinese traditional medicine dispensing liquid after cooling；
S3, be inoculated with lactobacillus buchneri into the Chinese traditional medicine dispensing liquid, ferment, then simultaneously be inoculated with Candida boidinii and
Senegal's acetic acid bacteria continues to ferment, filter after high-temperature inactivation, and the reject dregs of a decoction are to get draft ferment insecticide.
In the above-mentioned technical solutions, in step S1, in the decoction process twice, the additional amount of the water is the grass
8-12 times of draft Chinese medicine described in Chinese medicine.
Preferably, in step S1, the decoction process twice is to be decocted slowly using mild fire.
Preferably, in step S1, the decocting time twice is 60-180min.
Preferably, in step S1, the time of the immersion is 30-90min.
Further, in the above-mentioned technical solutions, in step S2, the carbohydrate is monosaccharide or polysaccharide, and additional amount is institute
0.02-0.024 times for stating draft Chinese medicine weight.
Preferably, in step S3, the fermentation time of the lactobacillus buchneri is greater than 48h, the Candida boidinii and
The fermentation time of Senegal's acetic acid bacteria is greater than for 24 hours.
Still further, in the above-mentioned technical solutions, the draft ferment method for producing insecticide, including walk as follows
It is rapid:
S1, draft Chinese medicine is weighed according to the ratio, and the water of described 8-12 times of weight of draft Chinese medicine is added, impregnate 45-75min,
After boiling 75-120min, decoction liquor of leaching, filter residue adds the boiling boiling 75- of described 8-12 times of weight of draft Chinese medicine
120min, and a decoction liquor addition is mixed into obtain Chinese medical extract；
S2, the carbohydrate that described 0.015-0.025 times of weight of draft Chinese medicine is added into the Chinese medical extract, mixing are adjusted
Match, be again heated to 121 DEG C, insulated sterilizing 10-20min is then cooled to 30-32 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, lactobacillus buchneri is inoculated with into the Chinese traditional medicine dispensing liquid, ferment 36-72h, then inoculates Bo Yiding vacation silk ferment
Female and Senegal's acetic acid bacteria continues the 18-30h that ferments, and is heated to 115 DEG C, 10-20min is kept the temperature, by the Chinese traditional medicine dispensing liquid
In viable bacteria inactivation, then filter, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
The present invention also provides the draft ferment insecticide or the preparation methods in preparation prevention and treatment cabbage caterpillar, diamondback moth and white
Application in the insecticide of aleyrodid.
Advantages of the present invention:
(1) draft ferment insecticide provided by the present invention passes through rationally matching to kuh-seng, the tuber of stemona, Fructus meliae toosendan and the fruit of Rangoon creeper
5, and using Candida boidinii, the co-fermentation of lactobacillus buchneri and Senegal's acetic acid bacteria, draft Chinese medicine is carried out pre-
Digestion is decomposed and is converted, and the intermediate material decomposition and inversion of macromolecular is become to the effective small-molecule substance that can be directly absorbed,
The absorption that can accelerate draft Chinese medicine, significantly effectively improves insecticidal effect；
(2) draft ferment insecticide provided by the present invention be free of any pesticide and chemical agent, have it is efficient, nontoxic,
The characteristics of harmless, pollution-free and noresidue, harmless to people, environment and water resource, the alternative existing chemical pesticide used, is one
The nuisanceless, environment-friendly pesticide of kind, can be used for preventing and treating the pest and disease damage of various crops, especially to same diamondback moth, cabbage caterpillar and powder
The insect pests such as lice class have significant control efficiency；
(3) draft ferment insecticide provided by the present invention system in such a way that heating decocts and mixed fermentation combines
It is standby, the active constituent in each draft Chinese medicine can be effectively extracted, required condition and equipment are simple, and simple process is controllable, have very
Important actual promotional value, theoretical and practical significance are great.
Specific embodiment
Combined with specific embodiments below, specific embodiments of the present invention will be described in further detail.
Following embodiment is only used for further illustrating the contents of the present invention, should not be construed as limiting the invention.Not
In the case where spirit of that invention and essence, to modifications or substitutions made by the method for the present invention, step or condition, this is belonged to
The range of invention.
Raw materials used source in the embodiment of the present invention and comparative example:
Draft Chinese medicine used is commercial product in the embodiment of the present invention and comparative example；
In the embodiment of the present invention and comparative example composite bacteria agent used the preparation method is as follows:
The Candida boidinii microbial inoculum, which is used, is preserved in China from safe (Wuhan) Biotechnology Co., Ltd of hundred scholars
Candida boidinii (the classification that Type Tissue Collection (Wuhan) deposit number is CCTCC M 2018162BST003
Name: Candida biodinin BST003, preservation address: China typical culture collection center (Wuhan), Wuhan, China-
Wuhan University, preservation date: on 03 29th, 2018) bacterial strain is made, and the specific method is as follows, aseptically, by Bo Yiding
Candida bacterial strain accesses on LB agar test tubes slant medium, the constant temperature incubation 66-78h at 30-35 DEG C, until growing obvious
Smooth bacterium colony to get Candida boidinii test tube strains, be placed in it is spare in 2-5 DEG C of refrigerator, then by LB liquid medium
It is fitted into and has dried in clean 1000ml triangular flask, per bottled amount 600-700ml, be stoppered tampon, wrap up bottleneck with brown paper,
Constant temperature sterilizing 30min, is cooled to 30-35 DEG C at 121 DEG C, aseptically the mature Candida boidinii examination of access culture
Pipe strain, the constant temperature incubation 36-72h at 135-170 rpm, 28-32 DEG C, until its viable count is greater than 5 × 107Cfu/g, juxtaposition
It is stand-by in 2-5 DEG C of refrigerator；
The lactobacillus buchneri microbial inoculum, which is used, is preserved in Chinese Typical Representative from safe (Wuhan) Biotechnology Co., Ltd of hundred scholars
Culture collection (Wuhan) deposit number be 2018164 BST005 of CCTCC M lactobacillus buchneri (classification naming:
Lactobacillus buchneri BST005, preservation address: China typical culture collection center (Wuhan), Wuhan, China-
Wuhan University, preservation date: on 03 29th, 2018) bacterial strain is made, and the specific method is as follows, aseptically, by Bu Shi cream
Bacillus strain accesses on sterilized MRS agar test tubes slant medium, and constant temperature incubation 66-78h is at 30-35 DEG C to get cloth
Family name's lactobacillus strain, be placed in it is spare in 2-5 DEG C of refrigerator, then by MRS fluid nutrient medium loading dried clean 1000ml tri-
In the bottle of angle, per bottled 600~700ml of amount, it is stoppered tampon, wraps up bottleneck with brown paper, constant temperature sterilizing 30min, cold at 121 DEG C
But to 30-35 DEG C, aseptically the mature lactobacillus buchneri strain of access culture, stands constant temperature incubation at 28-32 DEG C
36-72h, until its viable count is greater than 1 × 107Cfu/g is placed in 2-5 DEG C of refrigerator stand-by；
Senegal's acetic acid bacteria agent, which is used, is preserved in China from safe (Wuhan) Biotechnology Co., Ltd of hundred scholars
Senegal's acetic acid bacteria (classification that Type Tissue Collection (Wuhan) deposit number is CCTCC M 2018167BST008
Name: Acetobacter senegalensis BST008, preservation address: China typical culture collection center (Wuhan), in
State Wuhan-Wuhan University, preservation date: on 03 29th, 2018) bacterial strain is made, used Liquid Culture based formulas (quality
Than) are as follows: yeast extract 1%, glucose 1% and dehydrated alcohol 3%, used solid culture based formulas (mass ratio) are as follows: yeast
Powder 1%, glucose 1%, dehydrated alcohol 3% and agar powder 2% are soaked, the specific method is as follows, aseptically, by Senegal
Acetic acid bacteria strain accesses on sterilized Senegal's acetic acid bacteria solid test tube slant culture medium, the constant temperature incubation at 30-35 DEG C
66-78h to get lactobacillus buchneri strain, be placed in it is spare in 2-5 DEG C of refrigerator, will be in Senegal's acetic acid bacteria fluid nutrient medium
Yeast extract and glucose, which are fitted into, has dried in clean 1000ml triangular flask, per bottled 600~700ml of amount, is stoppered tampon, uses
Brown paper wraps up bottleneck, and constant temperature sterilizing 30min, is cooled to 30-35 DEG C at 121 DEG C, and aseptically addition 3% is anhydrous
Ethyl alcohol, access is trained ripe lactobacillus buchneri strain after shaking up, the constant temperature incubation 36-72h at 135-170rpm, 28-32 DEG C,
Until its viable count is greater than 1 × 107Cfu/g is placed in 2-5 DEG C of refrigerator stand-by.
If not particularly pointing out, technological means used in the embodiment of the present invention is well known to the skilled person normal
Rule means.
A kind of draft ferment insecticide of embodiment 1
1, raw material proportioning:
Draft Chinese medicine includes:
130 parts of kuh-seng, 128 parts of the tuber of stemona, 72 parts of Fructus meliae toosendan and 60 parts of the fruit of Rangoon creeper；
Composite bacteria agent includes:
0.48 part of Candida boidinii microbial inoculum, 0.52 part of lactobacillus buchneri microbial inoculum and Senegal's acetic acid bacteria agent
0.48-0.65 parts.
2, preparation method:
S1, draft Chinese medicine being weighed by said ratio, and the water of 12 times of weight of draft Chinese medicine being added, impregnate 60min, mild fire is slow
After decocting 90min, decoction liquor of leaching, the hydrology fire that filter residue adds 12 times of weight of draft Chinese medicine decocts 90min slowly, and will be primary
Decoction liquor addition mixes to obtain Chinese medical extract；
S2, the carbohydrate that 0.02 times of weight of draft Chinese medicine is added into Chinese medical extract, mixing preparation are again heated to 121 DEG C,
Insulated sterilizing 15min is then cooled to 30 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, lactobacillus buchneri is inoculated with into Chinese traditional medicine dispensing liquid, ferment 40h, then inoculates Candida boidinii and plug
Interior jar (unit of capacitance) acetic acid bacteria continues fermentation for 24 hours, is heated to 115 DEG C, keeps the temperature 15min, the viable bacteria in Chinese traditional medicine dispensing liquid is inactivated, then
Filtering, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
A kind of draft ferment insecticide of embodiment 2
1, raw material proportioning:
Draft Chinese medicine includes:
120 parts of kuh-seng, 125 parts of the tuber of stemona, 75 parts of Fructus meliae toosendan and 72 parts of the fruit of Rangoon creeper；
Composite bacteria agent includes:
0.66 part of Candida boidinii microbial inoculum, 0.52 part of lactobacillus buchneri microbial inoculum and Senegal's acetic acid bacteria agent 0.58
Part.
2, preparation method:
S1, draft Chinese medicine being weighed by said ratio, and the water of 10 times of weight of draft Chinese medicine being added, impregnate 60min, mild fire is slow
After decocting 90min, decoction liquor of leaching, the hydrology fire that filter residue adds 10 times of weight of draft Chinese medicine decocts 90min slowly, and will be primary
Decoction liquor addition mixes to obtain Chinese medical extract；
S2, the carbohydrate that 0.02 times of weight of draft Chinese medicine is added into Chinese medical extract, mixing preparation are again heated to 121 DEG C,
Insulated sterilizing 15min is then cooled to 30 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, lactobacillus buchneri is inoculated with into Chinese traditional medicine dispensing liquid, ferment 48h, then inoculates Candida boidinii and plug
Interior jar (unit of capacitance) acetic acid bacteria continues fermentation for 24 hours, is heated to 115 DEG C, keeps the temperature 15min, the viable bacteria in Chinese traditional medicine dispensing liquid is inactivated, then
Filtering, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
A kind of draft ferment insecticide of embodiment 3
1, raw material proportioning:
Draft Chinese medicine includes:
120 parts of kuh-seng, 98 parts of the tuber of stemona, 80 parts of Fructus meliae toosendan and 98 parts of the fruit of Rangoon creeper；
Composite bacteria agent includes:
0.75 part of Candida boidinii microbial inoculum, 0.82 part of lactobacillus buchneri microbial inoculum and Senegal's acetic acid bacteria agent 0.35
Part.
2, preparation method:
S1, draft Chinese medicine being weighed by said ratio, and the water of 10 times of weight of draft Chinese medicine being added, impregnate 60min, mild fire is slow
After decocting 120min, decoction liquor of leaching, filter residue adds the hydrology fire of 10 times of weight of draft Chinese medicine pan-fried 120min slowly, and by one
Secondary decoction liquor addition mixes to obtain Chinese medical extract；
S2, the carbohydrate that 0.02 times of weight of draft Chinese medicine is added into Chinese medical extract, mixing preparation are again heated to 121 DEG C,
Insulated sterilizing 15min is then cooled to 30 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, lactobacillus buchneri is inoculated with into Chinese traditional medicine dispensing liquid, ferment 54h, then inoculates Candida boidinii and plug
Interior jar (unit of capacitance) acetic acid bacteria continues the 30h that ferments, and is heated to 115 DEG C, keeps the temperature 15min, the viable bacteria in Chinese traditional medicine dispensing liquid is inactivated, then
Filtering, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
A kind of herbal insecticidal agent of comparative example 1
1, raw material proportioning:
Draft Chinese medicine includes:
118 parts of kuh-seng, 125 parts of the tuber of stemona, 72 parts of Fructus meliae toosendan and 75 parts of the fruit of Rangoon creeper.
2, preparation method:
S1, draft Chinese medicine being weighed by said ratio, and the water of 10 times of weight of draft Chinese medicine being added, impregnate 60min, mild fire is slow
After decocting 120min, decoction liquor of leaching, filter residue adds the hydrology fire of 10 times of weight of draft Chinese medicine pan-fried 120min slowly, and by one
Secondary decoction liquor addition mixes to obtain Chinese medical extract；
S2, Chinese medical extract is filtered into obtain the dregs of a decoction and traditional Chinese medicine liquid, the reject dregs of a decoction, gained traditional Chinese medicine liquid is herbal insecticidal
Agent.
A kind of draft ferment insecticide of comparative example 2
1, raw material proportioning:
Draft Chinese medicine includes:
115 parts of kuh-seng, 138 parts of the tuber of stemona, 66 parts of Fructus meliae toosendan and 75 parts of the fruit of Rangoon creeper；
Composite bacteria agent includes:
1.2 parts of 1.5 parts of Candida boidinii microbial inoculum, 0.48 part of lactobacillus buchneri microbial inoculum and Senegal's acetic acid bacteria agent
2, preparation method:
S1, draft Chinese medicine being weighed by said ratio, and the water of 10 times of weight of draft Chinese medicine being added, impregnate 60min, mild fire is slow
After decocting 90min, decoction liquor of leaching, the hydrology fire that filter residue adds 10 times of weight of draft Chinese medicine decocts 90min slowly, and will be primary
Decoction liquor addition mixes to obtain Chinese medical extract；
S2, the carbohydrate that 0.02 times of weight of draft Chinese medicine is added into Chinese medical extract, mixing preparation are again heated to 121 DEG C,
Insulated sterilizing 15min is then cooled to 30 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, lactobacillus buchneri is inoculated with into Chinese traditional medicine dispensing liquid, ferment 40h, then inoculates Candida boidinii and plug
Interior jar (unit of capacitance) acetic acid bacteria continues fermentation for 24 hours, is heated to 115 DEG C, keeps the temperature 15min, the viable bacteria in Chinese traditional medicine dispensing liquid is inactivated, then
Filtering, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
A kind of draft ferment insecticide draft Chinese medicine of comparative example 3 includes:
125 parts of kuh-seng, 110 parts of the tuber of stemona, 35 parts of Fructus meliae toosendan and 75 parts of the fruit of Rangoon creeper；
Composite bacteria agent includes:
0.55 part of Candida boidinii microbial inoculum, 0.48 part of lactobacillus buchneri microbial inoculum and Senegal's acetic acid bacteria agent 0.47
Part.
2, preparation method:
S1, draft Chinese medicine being weighed by said ratio, and the water of 10 times of weight of draft Chinese medicine being added, impregnate 60min, mild fire is slow
After decocting 120min, decoction liquor of leaching, filter residue adds the hydrology fire of 10 times of weight of draft Chinese medicine pan-fried 120min slowly, and by one
Secondary decoction liquor addition mixes to obtain Chinese medical extract；
S2, the carbohydrate that 0.02 times of weight of draft Chinese medicine is added into Chinese medical extract, mixing preparation are again heated to 121 DEG C,
Insulated sterilizing 15min is then cooled to 30 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, lactobacillus buchneri is inoculated with into Chinese traditional medicine dispensing liquid, ferment 72h, then inoculates Candida boidinii and plug
Interior jar (unit of capacitance) acetic acid bacteria continues the 48h that ferments, and is heated to 115 DEG C, keeps the temperature 15min, the viable bacteria in Chinese traditional medicine dispensing liquid is inactivated, then
Filtering, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
Insecticidal effect evaluation
Insecticide prepared by embodiment 1-3 and comparative example 1-3 is respectively adopted as reagent agent, designs 8 groups, every group
Healthy adult each 120 are selected, is individually positioned in indoor fresh Chinese cabbage leaf, 100 times is diluted with reagent agent and sprays.
Wherein, experimental group 1 is for trying adult with diamondback moth using insecticide made from embodiment 1 as reagent agent；Experiment
2-4 is using insecticide made from embodiment 2 as reagent agent for group, is respectively for trying adult with cabbage caterpillar, diamondback moth and trialeurodes vaporariorum；
Experimental group 5-8 respectively using insecticide made from embodiment 3 and comparative example 1-3 as reagent agent, with diamondback moth be for examination at
Worm；It is not administered as blank control group.
It carried out observing and recording test result at 1 day, 3 days, 5 days and 10 days respectively, as a result as shown in table 1 below.
From in the result of table 1 as can be seen that test example group 1-5 after spray in 10 days to include cabbage caterpillar, diamondback moth and
Pest including trialeurodes vaporariorum has preferable control efficiency, and Mortality of insect is 75% or more, and the 4-7 days after spray
Control efficiency is promoted comparatively fast, and control efficiency steadily increases to optimum efficiency behind the 7th day after spray；In contrast, test example
The control efficiency of group 6-8 is then significantly lower than test example group 1-5.
In conclusion the present invention, according to Chinese pharmacology and microbial fermentation correlation theory, scientific compatibility utilizes Bo Yiding vacation
Silk yeast, lactobacillus buchneri and Senegal's acetic acid bacteria carry out predigestion, decomposition and conversion, obtained draft to draft Chinese medicine
Ferment insecticide has good insecticidal action, and its insecticidal effect is stablized effectively, can reach killing for existing conventional chemical medicament
Worm effect, and to other animal bodies and human body are non-stimulated and toxic effect, secondary pollution will not be caused to environment, be a kind of desinsection
It works well, is nuisanceless, noresidue draft ferment insecticide, especially having to insect pests such as same diamondback moth, cabbage caterpillar and aleyrodid classes
There is significant control efficiency, actual application prospect is wide.
Finally, being not intended to limit the scope of the present invention the above is only preferred embodiment of the invention.It is all this
Within the spirit and principle of invention, any modification, equivalent replacement, improvement and so on should be included in protection model of the invention
Within enclosing.
Claims (10)
1. a kind of draft ferment insecticide, which is characterized in that its active constituent is sent out by the extracted rear inoculation compound bacteria of draft Chinese medicine
Ferment forms；
The draft Chinese medicine includes:
Kuh-seng, the tuber of stemona, Fructus meliae toosendan and the fruit of Rangoon creeper；
The compound bacteria includes:
Candida boidinii, lactobacillus buchneri and Senegal's acetic acid bacteria.
2. draft ferment insecticide according to claim 1, which is characterized in that
The draft Chinese medicine is made of the raw material of following parts by weight:
80-150 parts of kuh-seng, 80-150 parts of the tuber of stemona, 50-100 parts of Fructus meliae toosendan and 50-100 parts of the fruit of Rangoon creeper；
And/or in the compound bacteria, the viable count ratio of Candida boidinii, lactobacillus buchneri and Senegal's acetic acid bacteria
Example is (5-7.5): (1-1.5): (1-1.5)；
Preferably, the compound bacteria is provided in the form of microbial inoculum, by containing viable count 5 × 107-7.5×107The Bo Yiding of cfu/g
0.8-1.2 parts of Candida microbial inoculum contains viable count 1.0 × 107-1.25×107The lactobacillus buchneri microbial inoculum 0.8-1.2 of cfu/g
Part and contain viable count 1.0 × 107-1.25×1070.8-1.2 parts of the Senegal's acetic acid bacteria agent compositions of cfu/g；
The additional amount of the composite bacteria agent is the 0.1-1wt% of the draft Chinese medicine total amount.
3. draft ferment insecticide according to claim 2, which is characterized in that
The draft Chinese medicine is made of the raw material of following parts by weight:
96-135 parts of kuh-seng, 112-142 parts of the tuber of stemona, 65-88 parts of Fructus meliae toosendan and 58-95 parts of the fruit of Rangoon creeper；
The compound bacteria is provided in the form of microbial inoculum, by containing viable count 5 × 107-7.5×107The Bo Yiding vacation silk ferment of cfu/g
Female microbial inoculum 0.89-1.08 parts, contain viable count 1.0 × 107-1.25×1070.83-1.02 parts of the lactobacillus buchneri microbial inoculum of cfu/g
With contain viable count 1.0 × 107-1.25×1070.94-1.15 parts of the Senegal's acetic acid bacteria agent compositions of cfu/g, it is described multiple
The additional amount of combined bacteria agent is the 0.4-0.9wt% of the draft Chinese medicine total amount.
4. draft ferment insecticide according to claim 3, which is characterized in that
The draft Chinese medicine is made of the raw material of following parts by weight:
102-128 parts of kuh-seng, 118-135 parts of the tuber of stemona, 68-82 parts of Fructus meliae toosendan and 62-88 parts of the fruit of Rangoon creeper；
The compound bacteria is provided in the form of microbial inoculum, by containing viable count 5 × 107-7.5×107The Bo Yiding vacation silk ferment of cfu/g
Female microbial inoculum 0.92-1.05 parts, contain viable count 1.0 × 107-1.25×1070.87-0.98 parts of the lactobacillus buchneri microbial inoculum of cfu/g
With contain viable count 1.0 × 107-1.25×1070.97-1.09 parts of the Senegal's acetic acid bacteria agent compositions of cfu/g, it is described multiple
The additional amount of combined bacteria agent is the 0.48-0.78wt% of the draft Chinese medicine total amount.
5. draft ferment insecticide according to claim 1-4, which is characterized in that
The deposit number of the Candida boidinii is CCTCC M 2018162BST003；
The deposit number of the lactobacillus buchneri is CCTCC M 2018164BST005；
The deposit number of Senegal's acetic acid bacteria is CCTCC M 2018167BST008.
6. a kind of draft ferment method for producing insecticide as described in any one in claim 1-5, which is characterized in that including such as
Lower step:
S1, draft Chinese medicine is weighed according to the ratio, the decoction liquor merged twice twice is added water to cook after immersion and obtains Chinese medical extract；
S2, carbohydrate, mixing preparation are added into the Chinese medical extract, high-temperature sterilization obtains Chinese traditional medicine dispensing liquid after cooling；
S3, it is inoculated with lactobacillus buchneri into the Chinese traditional medicine dispensing liquid, fermented, be then inoculated with Candida boidinii and Sai Nei simultaneously
Jar (unit of capacitance) acetic acid bacteria continues to ferment, filter after high-temperature inactivation, and the reject dregs of a decoction are to get draft ferment insecticide.
7. draft ferment method for producing insecticide according to claim 6, which is characterized in that in step S1,
In the decoction process twice, the additional amount of the water is 8-12 times of draft Chinese medicine described in the draft Chinese medicine；
And/or the decoction process twice is to be decocted slowly using mild fire；
And/or the decocting time twice is 60-180min；
And/or the time of the immersion is 30-90min.
8. draft ferment method for producing insecticide according to claim 6 or 7, which is characterized in that
In step S2, the carbohydrate is monosaccharide or polysaccharide, and additional amount is 0.02-0.024 times of the draft Chinese medicine weight；
And/or in step S3, the fermentation time of the lactobacillus buchneri is greater than 48h, the Candida boidinii and Sai Neijia
The fermentation time of your acetic acid bacteria is greater than for 24 hours.
9. according to the described in any item draft ferment method for producing insecticide of claim 6-8, which is characterized in that including as follows
Step:
S1, draft Chinese medicine is weighed according to the ratio, and the water of described 8-12 times of weight of draft Chinese medicine is added, impregnate 45-75min, boil
After 75-120min, decoction liquor of leaching, filter residue adds the boiling boiling 75-120min of described 8-12 times of weight of draft Chinese medicine,
And a decoction liquor addition is mixed into obtain Chinese medical extract；
S2, the carbohydrate that described 0.015-0.025 times of weight of draft Chinese medicine is added into the Chinese medical extract, mixing preparation, then
121 DEG C are heated to, insulated sterilizing 10-20min is then cooled to 30-32 DEG C, obtains Chinese traditional medicine dispensing liquid；
S3, be inoculated with lactobacillus buchneri into the Chinese traditional medicine dispensing liquid, ferment 36-72h, then inoculate Candida boidinii and
Senegal's acetic acid bacteria continues the 18-30h that ferments, and is heated to 115 DEG C, 10-20min is kept the temperature, by the work in the Chinese traditional medicine dispensing liquid
Bacterium inactivation, is then filtered, the reject dregs of a decoction, gained Chinese medicine fermented liquid is draft ferment insecticide.
10. the described in any item draft ferment insecticides of claim 1-5 or the described in any item preparation methods of claim 6-9
Application in the insecticide of preparation prevention and treatment cabbage caterpillar, diamondback moth and trialeurodes vaporariorum.
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