CN108464317A - A kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation - Google Patents
A kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation Download PDFInfo
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- CN108464317A CN108464317A CN201810212116.XA CN201810212116A CN108464317A CN 108464317 A CN108464317 A CN 108464317A CN 201810212116 A CN201810212116 A CN 201810212116A CN 108464317 A CN108464317 A CN 108464317A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a kind of methods that peppermint and lemongrass fermentation prepare mosquito-repellent, include the following steps：(1) fresh mint leaf and lemon-grass are chosen respectively, and water crushing and beating is added, takes slurries spare；(2) bacillus licheniformis or bacillus thuringiensis are inoculated into culture medium, shake culture 12 for 24 hours, then takes medium centrifugal, the zymophyte bacterium solution after being centrifuged；(3) after slurries obtained being carried out sterilization treatment, zymophyte bacterium solution is added, carries out fermented and cultured, when zymotic fluid pH reduces by 0.5, end fermentation, then stored refrigerated 35 days；(4) by the zymotic fluid after refrigeration, coarse filtration is carried out, sterilizes, the stillness of night is collected by centrifugation to get mosquito-repellent.The present invention prepares mosquito-repellent using the method extraction active material of fermentation, easy to operate, without organic solvent and does not influence the stability of active material；The mosquito-repellent effective active composition content being prepared is high, can effectively prevent mosquito bite and insect bite and effect is lasting, environmental protection safe to use.
Description
Technical field
The invention belongs to technical field of biological fermentation more particularly to a kind of peppermint to prepare mosquito-repellent with lemongrass fermentation
Method.
Background technology
Mosquito be propagate the important medium of disease, the whole world is more than every year 2000000000 by the number that killing propagation disease threatens, because
This protection bite by mosquitos is significant.
Peppermint is Labiatae Mentha perennial root herbaceous plant, and stem is upright, high 30-60cm, and the several sections of tools in lower part are fine
Thin fibrous root and level is crawled root-like stock, sharp four diamond shape, has four slots, and micro- pubescence is swung on top, lower part only along water chestnut by pubescence,
Multi-branched.The long round shape lanceolar of blade, long 3-5cm, wide 0.8-3cm, the sharp point of apex, about 5-6 pairs of lateral vein.Umbrella inflorescence axillary is taken turns,
Profile is spherical, corolla lavender.The month at florescence 7-9, fruiting period October.The main place of production is the U.S., Spain, Italy, France, English
State, Balkan Peninsula etc., and most of China place such as Yunnan, Jiangsu, Zhejiang, Jiangxi etc. produces.
Lemongrass be grass family Cymbopogon plants, perennial herb, complete stool have lemon fragrance, 100-200 centimeters of plant height,
Colyliform is saved in the short Tibetan what underground of stem, and leaf sheath armful stem is hairless, and linear leaf goes out from close to root director, and leaf is 60-150 centimeters long, and wide about 1 is public
Point or so, blade fasciation, the two sides back of the body is coarse, and white, autumn and winter yields positive results, Hua Buxing, panicle evacuation, for majority
Raceme forms, caryopsis.Tropical Asia such as India, Sri Lanka are originated in, present Guangdong, Hainan, Taiwan are cultivated, extensive kind
It plants in torrid areas.It can be used for treating rheumatism effect, migraine.With anti-infective, improve digestion, deodorization, function for expelling pests can restrain
Skin, assigns freshness at the greasy unclean skin of conditioning, restores balance of body and spirit.
There is preferable repelling effect as botanical pesticide peppermint and lemongrass, but traditional botanical pesticide is multi-purpose
Organic solvent decocts extraction, and 1) it is unfavorable for energy saving also having larger environmental risk；2) stability and something lost of active material are influenced
Inactive materials；3) increasing cost makes development and application difficulty larger, and function and effect are slow, therefore grinding to plant insecticide
Hair and production bring huge financial difficulties.
Invention content
It is an object of the invention to：In view of the above problems, it a kind of peppermint is provided prepares mosquito with lemongrass fermentation and drive
The method for keeping away agent；The present invention uses the method extraction active material by peppermint and lemongrass fermentation to prepare mosquito-repellent, operation letter
It is single, without organic solvent and do not influence the stability of active material；The mosquito-repellent effective active composition content being prepared
Height can effectively prevent mosquito bite and insect bite, and mosquito-proof effect is lasting, environmental protection safe to use.
In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is as follows：
A kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation, includes the following steps：
(1) fresh mint leaf and lemon-grass are chosen respectively, after cleaning up, water crushing and beating are added, takes slurries spare；Institute
The mass ratio for stating dried peppermint leaf, lemon-grass and water is 1-5：1-5：5-10；
(2) bacillus licheniformis or bacillus thuringiensis are inoculated into culture medium, they are 28-32 DEG C in temperature, stirring
Rotating speed is shake culture 12-24h under conditions of 80-120rpm, then culture solution is taken to be centrifuged under conditions of 6500-7000rpm,
Zymophyte bacterium solution after being centrifuged；
(3) after slurries made from step (1) being carried out sterilization treatment, the zymophyte bacterium solution of step (2) is added, ferments
Culture terminates fermentation, then stored refrigerated 3-5 days under conditions of 5-10 DEG C when the pH of zymotic fluid reduces by 0.5；
(4) zymotic fluid after refrigerating step (3) carries out coarse filtration, sterilizes, is collected by centrifugation the stillness of night and driven to get the mosquito
Keep away agent.
The present invention is used as by using bacillus licheniformis or bacillus thuringiensis as Promoting bacteria fermentation peppermint and perfume (or spice)
Thatch extracts effective active composition, not only contributes to the extraction of bioactive substance, and due to having in fermentation process
Solvent and prolonged high-temperature process, do not easily cause the inactivation of active ingredient active material, advantageously ensure that peppermint and
Ingredients Active with mosquito repellent function in lemongrass.The bacillus licheniformis or bacillus thuringiensis of use are as Promoting bacteria itself
Also preferable disinsection prophylaxis and growth-promoting functions are conducive to the effect for enhancing mosquito repellent, and organic molten without adding in fermentation process
Agent etc. reduces the harm to environment；Using fresh dried peppermint leaf and lemon-grass, active constituent content is higher；And dried peppermint leaf,
Lemon-grass and water break into slurries using this range mass ratio, and the utilization rate of raw material can be improved, ensure the content of effective active composition.
Preferably, in step (1), the dried peppermint leaf and lemon-grass are crushed to 30-60 mesh.
Preferably, in step (2), the culture medium is made of the raw material of following weight percent：Sucrose 1.5%, albumen
Peptone 1.2%, sodium chloride 0.8%, amino acid 0.01%, water surplus；The culture medium sterilizes 15min under 121 DEG C of high pressures, and adjusts
Whole pH to 7.0.Using this medium culture bacillus licheniformis or bacillus thuringiensis, it is possible to provide preferable growing environment,
Effectively improve zymogenic activity, and zymogenic effective content in holding system.
Preferably, in step (2), a concentration of 110-115CFU/mL of the zymophyte bacterium colony.
Preferably, in step (3), the mass ratio of the slurries and zymophyte bacterium solution is 80-100：1-5.
Preferably, in step (3), the sterilization treatment is that slurries is made to carry out sterilization treatment under conditions of 90-100 DEG C
10-30min.The competitive growth that sterilization treatment can avoid other symbiotic microorganisms is carried out before fermentation, ensures the lichens gemma of inoculation
The growth of bacillus or bacillus thuringiensis, and then the extraction of effective active composition can be improved.
Further include that cellulase is added into slurries preferably, after the sterilization treatment, the dosage of the cellulase
For the 0.5-1.0% of slurries gross mass.The degradation that can promote fibr tissue in peppermint and lemongrass using cellulase degradation, from
And can make zymophyte more fully using them as nutriment, to can also improve the release of active ingredient and carry
It takes.
Preferably, in step (3), the temperature of the fermented and cultured is 28-35 DEG C.
Preferably, in step (4), the rotating speed of the centrifugation is 3500-4000rpm, centrifugation time 10-15min.
Peppermint mainly contains volatile oil, the wherein menthols such as menthol (also known as menthol), menthones and accounts for 62%, and menthones accounts for
15%, isomenthone accounts for 6%, also contain other volatile oil, as piperitenone, limonene, myrcene, terpane, peppermint esters,
Catechu naphthols acid, blue sesame oil hydrocarbon etc., the volatile oil active constituent that peppermint contains easily dissipates, and then mosquito is dispersed；Chinese medicine thinks
Peppermint acrid flavour cool in nature, have dispelling wind-heat, refresh oneself, promoting eruption work(, there is excited brain, promote blood circulation, sweating, anti-inflammatory town
Bitterly, the effect of anti-itch detoxification and dispelling wind and heat from the body.
Lemongrass has citrus scented, and distributes anophelifuge substance citronellal under field conditions (factors), reaches repelling effect, smell
Fragrance has ecological mosquito repellent, purification air, refreshes the mind, antibiotic and sterilizing and other effects.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
(1) present invention utilizes fermentation engineering, by selecting suitable strain and fermentation condition, from peppermint and lemongrass
Extract active material, on the one hand, it can utmostly retain the active constituent of peppermint and lemongrass, and relative to traditional water extraction,
Avoid the introducing of organic solvent, safety and environmental protection；On the other hand, it is not required to, by processes such as high-temperature extractions, avoid volatilizing in peppermint
Oily isoreactivity substance is lost, and influences the stability of active material, not only saves production cost, but also maximumlly simplifies production
Step enables the fermentation technique to realize mass production, industrialized production, and can fully ensure that the stability of product quality.
(2) fermentation process through the invention, in peppermint the extraction rate reached of the volatile oil such as menthol, menthones to 75% with
On, in lemongrass the total recovery rate of citronellal isoreactivity ingredient be 70% or more, from prepared by mosquito-repellent effective active composition
Content is high, can effectively prevent bite by mosquitos, mosquito-proof effect is high, and the duration is long, environmental protection safe to use.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention more comprehensible, preferred embodiment is enumerated below, to this hair
Bright further description.However, it is necessary to illustrate, many details listed in specification are used for the purpose of making reader to this
There are one thorough explanations for the one or more aspects of invention, can also realize the present invention's even without these specific details
These aspects.
Embodiment 1
A kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation, comprises the steps of：
(1) fresh mint leaf and lemon-grass are chosen respectively, after cleaning up, water crushing and beating are added, takes slurries spare；It is thin
Lotus leaf and lemon-grass are crushed to 45 mesh, and the mass ratio of the dried peppermint leaf, lemon-grass and water is 3：2：8；
(2) bacillus licheniformis is inoculated into culture medium, is 30 DEG C in temperature, under conditions of speed of agitator is 100rpm
Shake culture 20h, then takes culture solution to be centrifuged under conditions of 6800rpm, the zymophyte bacterium solution after being centrifuged；Culture medium by
The raw material of following weight percent is made：Sucrose 1.5%, peptone 1.2%, sodium chloride 0.8%, amino acid 0.01%, more than water
Amount；The culture medium sterilizes 15min under 121 DEG C of high pressures, and adjusts pH to 7.0；A concentration of 110CFU/mL of fermentation bacterium colony；
(3) after slurries made from step (1) being carried out sterilization treatment, the zymophyte bacterium solution of step (2), slurries and hair is added
The mass ratio of yeast-like fungi bacterium solution is 90：3, fermented and cultured is then carried out, the temperature of fermented and cultured is 30 DEG C；When the pH of zymotic fluid is reduced
When 0.5, terminate fermentation, then stored refrigerated 4 days under conditions of 8 DEG C；Sterilization treatment be make slurries under conditions of 95 DEG C into
Row sterilization treatment 20min；
(4) zymotic fluid after refrigerating step (3) carries out coarse filtration, sterilizes, is collected by centrifugation the stillness of night and driven to get the mosquito
Keep away agent；The rotating speed of centrifugation is 3800rpm, centrifugation time 12min.
The mosquito-repellent that the present embodiment is obtained carries out relevant effective active composition test, the results showed that mosquito repelling
Contain effective active composition volatile oil in agent, specifically contains menthol, menthones, isomenthone, limonene, myrcene, lemongrass
Aldehyde isoreactivity ingredient, wherein the extraction rate reached of the volatile oil such as menthol, menthones is to 78.5% in peppermint, citronellal in lemongrass
The total recovery rate of isoreactivity ingredient is 73.8%, to keep active constituent in this mosquito-repellent higher, has good mosquito proof
Effect.
Embodiment 2
A kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation, comprises the steps of：
(1) fresh mint leaf and lemon-grass are chosen respectively, after cleaning up, water crushing and beating are added, takes slurries spare；It is thin
Lotus leaf and lemon-grass are crushed to 30 mesh, and the mass ratio of the dried peppermint leaf, lemon-grass and water is 5：1：5；
(2) bacillus licheniformis is inoculated into culture medium, is 28 DEG C in temperature, under conditions of speed of agitator is 80rpm
Shake culture 12h, then takes culture solution to be centrifuged under conditions of 6500rpm, the zymophyte bacterium solution after being centrifuged；Culture medium by
The raw material of following weight percent is made：Sucrose 1.5%, peptone 1.2%, sodium chloride 0.8%, amino acid 0.01%, more than water
Amount；The culture medium sterilizes 15min under 121 DEG C of high pressures, and adjusts pH to 7.0；A concentration of 115CFU/mL of fermentation bacterium colony；
(3) after slurries made from step (1) being carried out sterilization treatment, the zymophyte bacterium solution of step (2), slurries and hair is added
The mass ratio of yeast-like fungi bacterium solution is 80：1, fermented and cultured is then carried out, the temperature of fermented and cultured is 28 DEG C；When the pH of zymotic fluid is reduced
When 0.5, terminate fermentation, then stored refrigerated 3 days under conditions of 5 DEG C；Sterilization treatment be make slurries under conditions of 90 DEG C into
Row sterilization treatment 30min；
(4) zymotic fluid after refrigerating step (3) carries out coarse filtration, sterilizes, is collected by centrifugation the stillness of night and driven to get the mosquito
Keep away agent；The rotating speed of centrifugation is 3500rpm, centrifugation time 15min.
The mosquito-repellent that the present embodiment is obtained carries out relevant effective active composition test, the results showed that mosquito repelling
Contain effective active composition volatile oil in agent, specifically contains menthol, menthones, isomenthone, limonene, myrcene, lemongrass
Aldehyde isoreactivity ingredient, wherein the extraction rate reached of the volatile oil such as menthol, menthones is to 76.2% in peppermint, citronellal in lemongrass
The total recovery rate of isoreactivity ingredient is 72.5%, to keep active constituent in this mosquito-repellent higher, has good mosquito proof
Effect.
Embodiment 3
A kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation, comprises the steps of：
(1) fresh mint leaf and lemon-grass are chosen respectively, after cleaning up, water crushing and beating are added, takes slurries spare；It is thin
Lotus leaf and lemon-grass are crushed to 60 mesh, and the mass ratio of the dried peppermint leaf, lemon-grass and water is 1：5：10；
(2) bacillus licheniformis is inoculated into culture medium, is 32 DEG C in temperature, under conditions of speed of agitator is 120rpm
Shake culture for 24 hours, then takes culture solution to be centrifuged under conditions of 7000rpm, the zymophyte bacterium solution after being centrifuged；Culture medium by
The raw material of following weight percent is made：Sucrose 1.5%, peptone 1.2%, sodium chloride 0.8%, amino acid 0.01%, more than water
Amount；The culture medium sterilizes 15min under 121 DEG C of high pressures, and adjusts pH to 7.0；A concentration of 115CFU/mL of fermentation bacterium colony；
(3) after slurries made from step (1) being carried out sterilization treatment, the zymophyte bacterium solution of step (2), slurries and hair is added
The mass ratio of yeast-like fungi bacterium solution is 100：5, fermented and cultured is then carried out, the temperature of fermented and cultured is 35 DEG C；When the pH of zymotic fluid drops
When low 0.5, terminate fermentation, then stored refrigerated 5 days under conditions of 10 DEG C；Sterilization treatment is the condition for making slurries at 100 DEG C
Lower progress sterilization treatment 10min；
(4) zymotic fluid after refrigerating step (3) carries out coarse filtration, sterilizes, is collected by centrifugation the stillness of night and driven to get the mosquito
Keep away agent；The rotating speed of centrifugation is 4000rpm, centrifugation time 10min.
The mosquito-repellent that the present embodiment is obtained carries out relevant effective active composition test, the results showed that mosquito repelling
Contain effective active composition volatile oil in agent, specifically contains menthol, menthones, isomenthone, limonene, myrcene, lemongrass
Aldehyde isoreactivity ingredient, wherein the extraction rate reached of the volatile oil such as menthol, menthones is to 75.7% in peppermint, citronellal in lemongrass
The total recovery rate of isoreactivity ingredient is 74.2%, to keep active constituent in this mosquito-repellent higher, has good mosquito proof
Effect.
Embodiment 4
The method of the present embodiment and embodiment 1 the difference is that：Embodiment 4 is after step (3) carries out sterilization treatment, also
Including cellulase is added into slurries, the dosage of the cellulase is the 0.5% of slurries gross mass, then adds step
(2) zymophyte bacterium solution carries out fermented and cultured；Remaining step and method and embodiment 1 are completely the same.
The mosquito-repellent that the present embodiment is obtained carries out relevant effective active composition test, the results showed that mosquito repelling
Contain effective active composition volatile oil in agent, specifically contains menthol, menthones, isomenthone, limonene, myrcene, lemongrass
Aldehyde isoreactivity ingredient, wherein the extraction rate reached of the volatile oil such as menthol, menthones is to 80.1% in peppermint, citronellal in lemongrass
The total recovery rate of isoreactivity ingredient is 76.8%, to keep active constituent in this mosquito-repellent higher, has good mosquito proof
Effect.
Embodiment 5
The method of the present embodiment and embodiment 1 the difference is that：Embodiment 4 is after step (3) carries out sterilization treatment, also
Including cellulase is added into slurries, the dosage of the cellulase is the 1.0% of slurries gross mass, then adds step
(2) zymophyte bacterium solution carries out fermented and cultured；Remaining step and method and embodiment 1 are completely the same.
The mosquito-repellent that the present embodiment is obtained carries out relevant effective active composition test, the results showed that mosquito repelling
Contain effective active composition volatile oil in agent, specifically contains menthol, menthones, isomenthone, limonene, myrcene, lemongrass
Aldehyde isoreactivity ingredient, wherein the extraction rate reached of the volatile oil such as menthol, menthones is to 79.6% in peppermint, citronellal in lemongrass
The total recovery rate of isoreactivity ingredient is 77.9%, to keep active constituent in this mosquito-repellent higher, has good mosquito proof
Effect.
Application method：After mosquito-repellent is diluted 1-3 times with ethyl alcohol, uniformly smears or be sprayed on human body or need repellent mosquito
On the object of worm.
Mosquito repellent is tested：
It is carried out according to GB/T17322.10-1998.Subject is 50 people, is divided into five groups, every group of 10 people, respectively to the present invention
The mosquito-repellent (diluting 1 times respectively) of embodiment 1-5 is tested.Everyone is blank control on the other hand, and the other hand presses 1.5mg/
cm2Dose uniformity smear 5cm × 5cm dorsal areas, exposure the wherein positions 4cm × 4cm, rest part tightly cover.It smears
Product 2 hours, proceeds by test.Test result is referring to table 1.
Table 1
From the data in table 1, it can be seen that the mosquito-repellent of 1-5 of the embodiment of the present invention meets A grades to aedes albopictus repellent effect,
Effective mosquito repellent time reaches 6 hours or more, wherein effective mosquito repellent time longest of embodiment 5, up to 6.5 hours, illustrates this hair
Bright mosquito-repellent can effectively prevent bite by mosquitos, and mosquito-proof effect is high, and the duration is long, environmental protection safe to use.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, it can also make several improvements and retouch, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of method that peppermint prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：Include the following steps：
(1) fresh mint leaf and lemon-grass are chosen respectively, after cleaning up, water crushing and beating are added, takes slurries spare；It is described thin
The mass ratio of lotus leaf, lemon-grass and water is 1-5：1-5：5-10；
(2) bacillus licheniformis or bacillus thuringiensis are inoculated into culture medium, they are 28-32 DEG C in temperature, speed of agitator
For shake culture 12-24h under conditions of 80-120rpm, then culture solution is taken to be centrifuged under conditions of 6500-7000rpm, obtained
Zymophyte bacterium solution after centrifugation；
(3) after slurries made from step (1) being carried out sterilization treatment, the zymophyte bacterium solution of step (2) is added, carries out fermentation training
It supports, when the pH of zymotic fluid reduces by 0.5, terminates fermentation, then stored refrigerated 3-5 days under conditions of 5-10 DEG C；
(4) zymotic fluid after refrigerating step (3) carries out coarse filtration, sterilizes, the stillness of night is collected by centrifugation to get the mosquito-repellent.
2. the method that peppermint according to claim 1 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：In step
(1), the dried peppermint leaf and lemon-grass are crushed to 30-60 mesh.
3. the method that fermentation prepares mosquito-repellent, it is characterised in that：In step (2), the culture medium is by following weight percent
The raw material of ratio is made：Sucrose 1.5%, peptone 1.2%, sodium chloride 0.8%, amino acid 0.01%, water surplus；The culture medium
Sterilize 15min under 121 DEG C of high pressures, and adjusts pH to 7.0.
4. the method that peppermint according to claim 1 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：In step
(2), a concentration of 110-115CFU/mL of the zymophyte bacterium colony.
5. the method that peppermint according to claim 4 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：In step
(3), the slurries and the mass ratio of zymophyte bacterium solution are 80-100：1-5.
6. the method that peppermint according to claim 1 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：In step
(3), the sterilization treatment is that slurries is made to carry out sterilization treatment 10-30min under conditions of 90-100 DEG C.
7. the method that peppermint according to claim 6 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：Described
Further include that cellulase is added into slurries after sterilization treatment, the dosage of the cellulase is the 0.5- of slurries gross mass
1.0%.
8. the method that peppermint according to claim 1 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：In step
(3), the temperature of the fermented and cultured is 28-35 DEG C.
9. the method that peppermint according to claim 1 prepares mosquito-repellent with lemongrass fermentation, it is characterised in that：In step
(4), the rotating speed of the centrifugation is 3500-4000rpm, centrifugation time 10-15min.
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