CN112266888A - Method for improving biomass and spore production of growth-promoting bacteria of bacillus plant roots - Google Patents
Method for improving biomass and spore production of growth-promoting bacteria of bacillus plant roots Download PDFInfo
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- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 33
- 241000196324 Embryophyta Species 0.000 title claims abstract description 29
- 241000894006 Bacteria Species 0.000 title claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000002028 Biomass Substances 0.000 title claims abstract description 21
- 235000019738 Limestone Nutrition 0.000 claims abstract description 21
- 239000006028 limestone Substances 0.000 claims abstract description 21
- 239000010902 straw Substances 0.000 claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 16
- 230000001737 promoting effect Effects 0.000 claims abstract description 16
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 12
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims abstract description 12
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims abstract description 11
- 229960000310 isoleucine Drugs 0.000 claims abstract description 11
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000007873 sieving Methods 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000001035 drying Methods 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- 238000001179 sorption measurement Methods 0.000 claims description 13
- 239000011812 mixed powder Substances 0.000 claims description 12
- 230000002786 root growth Effects 0.000 claims description 11
- 239000002893 slag Substances 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000002068 microbial inoculum Substances 0.000 claims description 7
- 240000008892 Helianthus tuberosus Species 0.000 claims description 2
- 235000003230 Helianthus tuberosus Nutrition 0.000 claims description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 8
- 239000002689 soil Substances 0.000 abstract description 6
- 230000012010 growth Effects 0.000 abstract description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 239000003337 fertilizer Substances 0.000 abstract description 3
- 239000011159 matrix material Substances 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000028070 sporulation Effects 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 241000194106 Bacillus mycoides Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 1
- 241000589157 Rhizobiales Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 239000007633 bacillus mucilaginosus Substances 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
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- C12N1/20—Bacteria; Culture media therefor
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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Abstract
The invention belongs to the technical field of microbial agents, and particularly relates to a method for improving biomass and spore production of bacillus plant root growth-promoting bacteria. Meanwhile, crop straws and manioc waste can also be used as fertilizer for plants, and can be degraded back to soil, so that nutrition is provided for the growth of the plants, and the fertilizer is environment-friendly and energy-saving. In addition, a carrier matrix formed by crop straws and manioc waste is mixed with limestone powder and isoleucine in advance, the limestone powder is rich in calcium carbonate components, and the calcium carbonate and the isoleucine promote bacillus to generate spores, so that the growth promoting effect of crops is improved.
Description
Technical Field
The invention belongs to the technical field of microbial agents, and particularly relates to a method for improving biomass and spore production of growth-promoting bacteria of a plant root system of bacillus.
Background
Plant growth-promoting rhizobacteria, which colonize the rhizosphere of plants, have the ability to promote plant growth by direct mechanisms such as soil nutrient solubilization and production of plant hormones, or by indirect mechanisms such as enzyme production, nutrient competition and production of systemic resistance. These microorganisms can be produced rapidly in the culture medium and can be stored for a long time in view of their spore production.
Various strains of some species of Bacillus (for example, Bacillus subtilis), Bacillus amyloliquefaciens, Bacillus cereus, Bacillus mycoides, Bacillus anthracis, Bacillus thuringiensis, Bacillus licheniformis, Bacillus natto, Bacillus megaterium, and Bacillus mucilaginosus) have been identified as useful in agricultural activities, and belong to plant growth promoting rhizosphere bacteria.
In the prior art, the growth promoting effect on crops is not ideal because the obtained biomass is low or the sporulation rate is low.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus, aiming at solving the technical problem that the growth-promoting effect on crops is not ideal because the obtained biomass is low or the spore formation rate is low in the prior art.
The invention provides a method for improving biomass and spore production of bacillus plant root growth-promoting bacteria, which comprises the following specific technical scheme:
the method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus comprises the following steps:
s1, crushing crop straws, mixing the crushed crop straws with cassava dregs, and drying, crushing and sieving the mixture in sequence to obtain mixed powder dregs;
s2, drying and sterilizing the mixed powder slag obtained in the step S1 to obtain an adsorption carrier;
s3, crushing, sieving and baking the limestone for two rounds, and sieving again to obtain limestone powder;
s4, mixing the adsorption carrier in the step S2 with the limestone powder and the isoleucine in the step S3 to obtain a carrier mixture;
s5, mixing the carrier mixture in the step S5 with the plant root growth promoting microbial inoculum of the bacillus.
In certain embodiments, in step S1, the crop straw and the cassava residue are mixed in a weight ratio of 1: (8-10) mixing, wherein the crop straws are jerusalem artichoke straws, the drying is to dry the mixed powder slag until the moisture mass content is 2-8%, and the sieved sieve mesh is 100-200 meshes.
In some embodiments, in step S2, the drying is to dry the adsorption carrier to a moisture content of 2-8%, the sterilization temperature is 115-121 ℃, and the sterilization time is 30-60 min.
In some embodiments, in step S3, the sieved mesh is 100-200 mesh, the baking temperature is 500-700 ℃, the baking time is 60-90min, and the sieved mesh is 100-200 mesh.
In certain embodiments, in step S4, the adsorbent carrier, the limestone powder and isoleucine are mixed in a weight ratio of 50: (5-10): (0.5-2) mixing.
In some embodiments, in step S5, the carrier mixture is 10 to 50 parts by weight, and the bacillus plant root growth promoting agent is 0.5 to 5 parts by weight.
The invention has the following beneficial effects: 1. the invention is universal for crop straws and cassava residues, and the raw materials with wide sources are used as a carrier matrix of the bacillus plant root growth promoting microbial inoculum, so the cost is low, and simultaneously the bacillus plant root growth promoting microbial inoculum is convenient to store and keeps the activity in transportation.
2. The crop straws and the cassava residues can also be used as fertilizers for plants, can be degraded back to soil, provide nutrition for the growth of the plants, and are environment-friendly and energy-saving.
3. According to the invention, the carrier matrix formed by the crop straws and the cassava residues is mixed with limestone powder and isoleucine in advance, the limestone powder contains abundant calcium carbonate components, and the calcium carbonate and the isoleucine promote the bacillus to produce spores, so that the growth promoting effect of crops is improved.
Drawings
FIG. 1 is a flow chart of the method of the present invention for increasing biomass and sporulation of growth-promoting bacteria of Bacillus plant roots.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings 1 in conjunction with specific embodiments.
Example 1
The method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus plant provided by the embodiment has the following specific technical scheme:
the method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus comprises the following steps:
s1, crushing crop straws, and mixing the crushed crop straws with the cassava dregs according to the weight ratio of 1: 8, drying (the water content is 2 percent), crushing and sieving (the sieve mesh is 100 meshes) in sequence to obtain mixed powder slag.
And S2, drying the mixed powder slag (the water content is 2 percent), and sterilizing (the sterilization temperature is 115 ℃ and the sterilization time is 60min) to obtain the adsorption carrier.
S3, crushing, sieving and baking the limestone in two rounds. Wherein the sieved mesh is 100 meshes, the baking temperature is 500 ℃, and the baking time is 90 min; and (4) sieving again with a sieve mesh of 100 meshes to obtain limestone powder.
S4, mixing the adsorption carrier, limestone powder and isoleucine according to the weight ratio of 50: 5: 0.5 to obtain a carrier mixture.
S5, mixing 10 parts of the carrier mixture with 0.5 part of the bacillus plant root growth promoting agent.
Example 2
The method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus plant provided by the embodiment has the following specific technical scheme:
the method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus comprises the following steps:
s1, crushing crop straws, and mixing the crushed crop straws with the cassava dregs according to the weight ratio of 1: 10, drying (the water content is 8 percent), crushing and sieving (the sieve mesh is 200 meshes) in sequence to obtain mixed powder slag;
s2, drying the mixed powder slag (the water content is 8 percent), and sterilizing (the sterilization temperature is 121 ℃, and the sterilization time is 30min) to obtain an adsorption carrier;
s3, crushing, sieving and baking the limestone for two times, wherein the sieved mesh is 200 meshes, the baking temperature is 700 ℃, and the baking time is 60 min; sieving again with 200 mesh sieve to obtain limestone powder;
s4, mixing the adsorption carrier, limestone powder and isoleucine according to the weight ratio of 50: 10: 2, mixing to obtain a carrier mixture;
s5, mixing 50 parts of the carrier mixture with 5 parts of the bacillus plant root growth promoting microbial inoculum.
Example 3
The method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus plant provided by the embodiment has the following specific technical scheme:
the method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus comprises the following steps:
s1, crushing crop straws, and mixing the crushed crop straws with the cassava dregs according to the weight ratio of 1: 9, mixing, drying (the water content is 7.2 percent), crushing, and sieving (the sieve mesh is 150 meshes) in sequence to obtain mixed powder slag;
s2, drying the mixed powder slag (the water content is 7.2%), sterilizing (the sterilization temperature is 121 ℃, and the sterilization time is 30min), and obtaining an adsorption carrier;
s3, crushing, sieving and baking the limestone for two times, wherein the sieve mesh is 150 meshes, the baking temperature is 600 ℃, and the baking time is 75 min; sieving again with 150 mesh sieve to obtain limestone powder;
s4, mixing the adsorption carrier, limestone powder and isoleucine according to the weight ratio of 50: 8: 1, mixing to obtain a carrier mixture;
s5, mixing 40 parts of the carrier mixture with 2 parts of the bacillus plant root growth promoting microbial inoculum.
In order to further verify the effects of the method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus, the following application experiments are carried out. According to the experiment, corns are used as an experimental object of a fertilization object, a pure bacillus plant root growth promoting microbial inoculum is used as a comparative example, other experimental conditions are consistent, the corn yield and the spore formation rate in planting soil are detected, and the experimental results are shown in table 1.
TABLE 1 corn yield and sporulation rate in planting soil
| Corn yield (kg) | Rate of sporulation (%) | |
| Example 3 | 46 | 92.8 |
| Comparative example | 37 | 67.4 |
In conclusion, the method provided by the invention can effectively promote the sporulation rate of the plant root growth-promoting bacteria of the bacillus in the soil, thereby further promoting the growth of plants and improving the yield.
The above description is only for the purpose of illustrating preferred embodiments of the present invention and is not to be construed as limiting the invention, and the present invention is not limited to the above examples, and those skilled in the art should also be able to make various changes, modifications, additions or substitutions within the spirit and scope of the present invention.
Claims (6)
1. The method for improving the biomass and spore production of the growth-promoting bacteria of the root system of the bacillus is characterized by comprising the following steps:
s1, crushing crop straws, mixing the crushed crop straws with cassava dregs, and drying, crushing and sieving the mixture in sequence to obtain mixed powder dregs;
s2, drying and sterilizing the mixed powder slag obtained in the step S1 to obtain an adsorption carrier;
s3, crushing, sieving and baking the limestone for two rounds, and sieving again to obtain limestone powder;
s4, mixing the adsorption carrier in the step S2 with the limestone powder and the isoleucine in the step S3 to obtain a carrier mixture;
s5, mixing the carrier mixture in the step S5 with the plant root growth promoting microbial inoculum of the bacillus.
2. The method for improving biomass and spore production of growth-promoting bacteria of plant roots of bacillus according to claim 1, wherein in step S1, the weight ratio of the crop straw to the cassava residue is 1: (8-10) mixing, wherein the crop straws are jerusalem artichoke straws, the drying is to dry the mixed powder slag until the moisture mass content is 2-8%, and the sieved sieve mesh is 100-200 meshes.
3. The method as claimed in claim 1, wherein the drying step S2 is to dry the adsorption carrier to a moisture content of 2-8%, the sterilization temperature is 115-121 ℃, and the sterilization time is 30-60 min.
4. The method as claimed in claim 1, wherein in step S3, the sieved mesh is 200 meshes 100-.
5. The method for improving biomass and spore production of Bacillus plant root growth-promoting bacteria according to claim 1, wherein in step S4, the adsorption carrier, the limestone powder and isoleucine are mixed in a weight ratio of 50: (5-10): (0.5-2) mixing.
6. The method for improving biomass and spore production of Bacillus plant root growth promoting bacteria according to claim 1, wherein in step S5, the carrier mixture is 10-50 parts by weight, and the Bacillus plant root growth promoting bacteria is 0.5-5 parts by weight.
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