CN112167048A - Breeding and breeding method for hemerocallis fulva - Google Patents

Breeding and breeding method for hemerocallis fulva Download PDF

Info

Publication number
CN112167048A
CN112167048A CN202010810861.1A CN202010810861A CN112167048A CN 112167048 A CN112167048 A CN 112167048A CN 202010810861 A CN202010810861 A CN 202010810861A CN 112167048 A CN112167048 A CN 112167048A
Authority
CN
China
Prior art keywords
breeding
selecting
hemerocallis
resistant
hemerocallis fulva
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010810861.1A
Other languages
Chinese (zh)
Inventor
李伟
陈伟
段仕学
文凤
刘根果
李玉玲
张云宏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yunnan Jicheng Landscape Technology Co ltd
Original Assignee
Yunnan Jicheng Landscape Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yunnan Jicheng Landscape Technology Co ltd filed Critical Yunnan Jicheng Landscape Technology Co ltd
Priority to CN202010810861.1A priority Critical patent/CN112167048A/en
Publication of CN112167048A publication Critical patent/CN112167048A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention belongs to the field of plant breeding, and particularly discloses a method for breeding and breeding hemerocallis fulva, which comprises the steps of collecting hemerocallis fulva germplasm resources, evaluating and screening disease-resistant germplasm, screening evergreen and disease-resistant germplasm suitable for regional climate and soil conditions, analyzing the ploidy of hemerocallis fulva germplasm chromosomes, selecting tetraploid large flower type or diploid small flower dwarf greening varieties, selecting evergreen, disease-resistant and heat-resistant target traits by utilizing a molecular marker technology, finally carrying out directional breeding on hemerocallis fulva new variety through hybridization breeding, carrying out plant tissue culture and propagation on the obtained variety, rapidly screening parent plants with required traits by the method, greatly keeping genetic substances of the parent plants in a tissue culture mode, not generating trait change in a repeated planting process, and finally obtaining the variety with the target traits through breeding and analyzing the ploidy, the molecular marker assisted screening method greatly shortens the time of conventional breeding.

Description

Breeding and breeding method for hemerocallis fulva
Technical Field
The invention belongs to the field of plant breeding, and particularly relates to a method for breeding and breeding hemerocallis fulva.
Background
Hemerocallis (Hemerocallis) is a perennial herb flower of Hemerocallis of Liliaceae, native areas are about 14 in China, south Europe and Japan, native areas of China are about 11, and native areas are distributed in the south and north of China. The hemerocallis fulva has developed root system, is cold-resistant, drought-resistant and barren-resistant, has strong environmental adaptability, extensive management and low maintenance cost, and is an excellent landscaping plant. The flowers of the hemerocallis fulva are similar to lilies, the leaves are similar to orchid, the variety is wide, the flower types are various, the flower color is rich, the ornamental value is high, the flower is positioned at the head of three perennial flowers (hemerocallis fulva, hosta plantaginea and iris tectorum), and the flower is widely used for road greening, park greenbelt, yard landscaping, water and soil conservation, roof garden beautification, balcony beautification and the like.
In 1893, George-yeld registered the first daylily cultivar Aprioot. After 20 th century, some botanical orchards, universities and gardening companies in Europe and America began to collect plants of Hemerocallis in China, such as Japan, and hybridized with local Hemerocallis, so that new varieties are emerging. The plant type has mini type and short type varieties with the plant height of 20-30 cm; from the flower diameter, the flower is small in size of 5cm to large in size of more than 20 cm; the yellow and orange of the original seed are broken through from the flower color, and a series of red, purple, pink, orange, apricot, black and the like appear; in the green stage, there are evergreen, semi-evergreen and deciduous leaf types; the flower type is of single petal type and double petal type, and the variety of the hemerocallis is greatly enriched.
Up to now, more than 6 thousands of varieties have been registered internationally, and become one of the most cultivated varieties in all the perennial flowers. However, pure blue, green and white hemerocallis have not appeared in the aspect of colors. In terms of flower fragrance, although there are strong-fragrance groups in wild hemerocallis fulva species, such as hemerocallis fulva and north hemerocallis fulva, through multi-generation hybrid breeding, the fragrance character is gradually lost in modern hemerocallis fulva varieties, and most hemerocallis fulva varieties have no fragrance or only a very light fragrance. Therefore, the cultivation of aromatic hemerocallis fulva is one of the future breeding directions. In the flowering period, new species are more and more blossoming, but the single flower of hemerocallis takes 1 day. Some day lily which blooms at night withers in the morning on day 2, and the day is basically not seen to be opened, so that the ornamental value is lost. Therefore, the single flower open time of the hemerocallis fulva and the prolongation of the group flowering phase are also important directions for hemerocallis fulva breeding. In the aspect of flower type, the flower color of the day lily is changeable, the day lily can be respectively suitable for different purposes, and the cultivation of more abundant day lily varieties by utilizing the existing variety resources is also the direction of breeding in the future. In the aspect of resistance, due to the difference of different regional climates and the difference between hemerocallis varieties, the resistance of each variety to a certain disease and insect pest is different, so that the breeding of disease-resistant and insect-resistant varieties suitable for local climatic conditions has important significance for improving the ornamental quality of the hemerocallis and reducing the maintenance cost. The full bloom stage of the hemerocallis is concentrated in the midsummer, the varieties of early flowers and late flowers are fewer and the ornamental value is not high, so that the cultivation of more excellent early flowers and late flowers and the varieties capable of continuously flowering are hot spots of breeding. Day lily is seen in spring and autumn and flower in summer, but most varieties of day lily are withered in winter, so that winter appreciation of day lily is directly influenced, and application of day lily in home gardening and urban greening is influenced. Therefore, cultivation of evergreen varieties is also one of the breeding directions of hemerocallis.
In recent years, hybrid breeding is carried out by units such as institute of sciences in China, Beijing forestry university, Beijing orchard, Beijing garden research institute, Xian botanical garden and the like on the basis of collecting new varieties, and new varieties are published. The Hokka group of Beijing university of forestry made progress in secondary flowering gene screening on the molecular level.
The units of Shanghai application technology university, Shanghai garden science research institute, Shanghai vegetable garden, Shanghai Chengshan vegetable garden, Shanghai city delivery company, Shanghai gardening company and the like successively do a lot of work on the aspects of introduction evaluation of hemerocallis, tissue culture propagation expansion, construction of hemerocallis special gardens and the like. The Shanghai is in the east of Yangtze river delta in Shanghai, belongs to the tropical monsoon climate in North Asia, and the light and temperature conditions are suitable for the growth of hemerocallis. And moreover, the hemerocallis fulva has strong tolerance to saline-alkali soil, can adapt to the characteristic of slightly alkaline soil in the Shanghai, and has great popularization and planting potential in the Shanghai area. At present, the number of excellent varieties of hemerocallis popularized and applied in Shanghai province is less than 10, the color is single, the disease resistance is poor, the stock quantity of seedlings is small, and the demand of urban greening can not be met.
With the rapid growth of economy and improvement of living standard of people in China, garden greening in China has already passed through a rapid development period. With the increasing level of greening and the increasing awareness of ecology, people are beginning to recognize that more types of "lower trees (lawns, groundcover and perennial flowers)" are required in addition to "upper trees (trees, shrubs)"; besides the first-and second-year-old herbaceous flower bed flowers, excellent perennial root flowers are needed to enrich the landscape and reduce the cost; from an ecological perspective, more types of flowers are required to enrich species diversity, and more perennial flowers and ground cover plants with developed root systems are required to manage extensive land and soil erosion prevention and energy consumption reduction.
Therefore, on the basis of the wide introduction of new day lily varieties at home and abroad, the new day lily variety with gorgeous colors and disease resistance is cultivated through breeding means such as hybridization, and the method has important significance for enriching the garden greenbelt landscape, increasing new family gardening flower varieties and promoting the role of the day lily in ecological environment construction.
Disclosure of Invention
The invention mainly aims to provide a method for breeding hemerocallis fulva to solve the problems in the background technology.
In order to achieve the purpose of the invention, the invention provides the following technical scheme:
a method for breeding hemerocallis fulva comprises the following steps:
(1) collecting and evaluating hemerocallis fulva germplasm resources, screening disease-resistant germplasm, and screening evergreen and disease-resistant germplasm suitable for regional climate and soil conditions;
(2) selecting and breeding a fine variety of the hemerocallis, including chromosome ploidy analysis of the hemerocallis germplasm, selecting a tetraploid large flower type or diploid small flower dwarf greening variety, then selecting evergreen, disease-resistant and heat-resistant target traits by utilizing a molecular marker technology, and finally carrying out directional breeding on a new variety of the hemerocallis through cross breeding;
(3) and carrying out plant tissue culture and propagation on the obtained variety.
Preferably, the method for breeding and breeding hemerocallis fulva further comprises the following steps:
(1) selecting a parent material A of red pirate hemerocallis fulva and a parent material B of golden baby hemerocallis fulva to plant in Jianghu and Zhejiang Shanghai;
(2) selecting dominant plants in the planting area, taking flower stems of the dominant plants as explants, and carrying out tissue culture and transplanting to fields to obtain F1 generations A and B;
(3) carrying out chromosome ploidy analysis on the dominant plant, selecting red pirates with tetraploid large flower types and dolls with diploid small flower types, and selecting evergreen, disease-resistant and heat-resistant target characters by utilizing molecular markers;
(4) and (3) performing two positive and negative hybridization modes of A multiplied by B and B multiplied by A on the parent material A and the parent material B of the selected plant F1 to respectively obtain hemerocallis hybrid varieties AF1 and BF1, performing ploidy analysis and molecular marker selection again to obtain the optimal variety AF1, and performing tissue culture propagation.
Preferably, the method for breeding and selecting hemerocallis fulva comprises the following steps:
(1) selecting flower stem as explant, washing with tap water for 30 min, sterilizing with 5% ethanol for 30s, and adding 0.1% HgCl2Soaking for 8 min;
(2) primary culture, wherein the callus induction culture medium is MS +0.2mg/LNAA +2 mg/L6-BA;
(3) carrying out enrichment culture, wherein the adventitious bud is induced into MS +0.3mg/LNAA +1.0 mg/L6-BA;
(4) rooting culture, 1/2MS +0.2 mg/LNAA.
Preferably, the explants are sterilized and then transplanted to culture medium after 1 minute of soaking in 0.5% VC.
Preferably, the culture temperature is 25 ℃, the illumination intensity is 2000lx, and the illumination time is 12 h/d.
The invention has the following beneficial effects: by the method, parent plants with required characters can be quickly screened, the genetic materials of the parent plants are greatly maintained through a tissue culture mode, the characters cannot be changed in the repeated planting process, the finally bred variety has the target characters, the conventional breeding time is greatly shortened through the ploid analysis and molecular marker-assisted screening method, the two varieties selected by the method are bred and propagated in Jianghe and Zhejiang Shanghai, the obtained variety is evergreen, disease-resistant and heat-resistant, the flower color is pure, the flowering period reaches more than 3 days, and the tissue culture method used by the method has low pollution rate and basically no browning condition.
Detailed Description
In order to clearly understand the technical spirit and the advantages of the present invention, the applicant below describes in detail by way of example, but the description of the example is not intended to limit the technical scope of the present invention, and any equivalent changes made according to the present inventive concept, which are merely in form and not in material, should be considered as the technical scope of the present invention.
The test reagent consumables used in the following examples are all conventional biochemical reagents unless otherwise specified; the experimental methods are conventional methods unless otherwise specified, and the percentages are mass percentages unless otherwise specified.
Example 1
The main Yangtze triangle area of the parent cultivation area comprises 3 cities of Suzhou, Hangzhou and Shanghai. The test area is designed into 3 cells, the size of each cell is set according to the number of test materials, the test materials are repeated for 3 times, and protection rows are arranged on the periphery of each cell. Selecting grown plants with the same size, wherein the plant number/m 2 is 18, the plant row spacing is 30cm multiplied by 30cm, the plant varieties of red pirate hemerocallis parent material A and golden baby hemerocallis parent material B are red pirate parents supplied by the Yunnan flower market, and the golden baby hemerocallis are purchased locally. After cultivation, on the basis of collecting hemerocallis fulva varieties (leaf blight resistance, large flower, small flower and dwarf), the characteristics of the plants (green period, short plant height, flower type, flower color, flower diameter, scape height, flower period and meristematic capacity) are observed, and heat resistance, cold resistance and disease resistance are evaluated. Screening evergreen and disease-resistant germplasm suitable for climatic and soil conditions in Jiangzhe Shanghai region, and referring to the identification standard of hemerocallis middendorfii in Table 1.
TABLE 1. Hemerocallis fulva identification Standard
Figure BDA0002630913950000051
Selecting dominant plants in the planting area, taking flower stems of the dominant plants as explants, and carrying out tissue culture and transplanting to fields to obtain F1 generations A and B; carrying out chromosome ploidy analysis on the dominant plant, selecting red pirates with tetraploid large flower types and dolls with diploid small flower types, and selecting evergreen, disease-resistant and heat-resistant target characters by utilizing molecular markers; carrying out A multiplied by B and B multiplied by A positive and negative hybridization on a parent material A and a parent material B of a selected plant F1 to respectively obtain day lily hybrid varieties AF1 and BF1, carrying out ploidy analysis and molecular marker selection again to obtain the best variety AF1, and carrying out tissue culture propagation, wherein the specific conditions of the best variety are shown in tables 2 and 3, the flower size of the new variety is close to that of the female parent, the crown width is slightly smaller than that of the female parent, the flower stalk is shorter than that of the female parent, the flower color is brighter than that of the female parent, the flowering phase is longer than that of the female parent, and the flowering phase is 5-9 months. And the upper part of the ground can be kept evergreen in winter.
1. Hemerocallis germplasm chromosome ploidy analysis method
Aiming at the current situation that the introduction path of the hemerocallis fulva variety is disordered and the ploidy of the chromosome is unknown, the chromosome ploidy analysis is carried out before the parents are selected. The chromosome number is observed by a conventional root tip tabletting method. Taking about 1cm of root tip of hemerocallis fulva, pretreating the root tip with saturated p-dichlorobenzene aqueous solution at room temperature for 4h, taking out the root tip, fixing with Carnot fixing solution at 0 ℃ for 24h, dissociating with 1mol/L hydrochloric acid in a constant-temperature water bath at 60 ℃ for 8min, dyeing with Carbowax dye solution for 20min, and tabletting. And observing and counting the chromosome under a microscope, determining the chromosome ploidy of the hemerocallis germplasm, and selecting a hybrid parent according to the chromosome ploidy.
2. Selection of target characters such as evergreen and disease resistance by using molecular marker technology
Grouping the collected hemerocallis germplasm according to the target character type by using a group separation analysis method (BSA method), searching the difference of amplification bands among groups by using an AFLP (amplified fragment length polymorphism) molecular marker technology, quickly screening out molecular markers linked with target genes, determining the linkage relation among the molecular markers through linkage analysis, performing auxiliary selection on characters such as disease resistance and evergreen by using DNA molecular markers closely linked with the target characters, and finally aggregating a plurality of genes controlling different characters through conventional hybridization to ensure that a new variety has a plurality of excellent characters such as evergreen and disease resistance.
TABLE 2 comparison of best breed parents to progeny phenotypes
Figure BDA0002630913950000061
TABLE 3 comparison of the later stages of the New varieties with the progeny of the hybrids
Figure BDA0002630913950000062
3. Parent and hybrid seedling cultivation and planting method
3.1 plant row spacing
Transplanting the purchased seedlings for the 1 st time into a nutrition pot of 10cm multiplied by 12cm, transplanting the plants in the 2 nd autumn into a field for the 2 nd time, wherein the plant-row spacing is 30cm multiplied by 30 cm. Or directly transplanting into field at the 1 st transplanting time, wherein the row spacing of the plants at the 1 st transplanting time is 20cm multiplied by 20 cm.
3.2 fertilization
In the seedling regrowth stage, the fertilizer requirement is high, and the seedling is applied thinly and frequently. The fertilization can be combined with watering, and 0.2 percent of compound fertilizer is applied every 10 days. Fertilization of biennial seedlings in the growth period can be carried out 2-3 times, wherein the fertilization is carried out before the leaves germinate in 2 months in the 1 st time, before the leaves bloom in 5 months in the 2 nd time and before the leaves germinate in 9 months in the early autumn.
3.3 irrigation
After the new seedlings are transplanted, the soil water holding capacity needs to be maintained at 70% -80%, and watering is carried out in time during drought. The plant is small in seedling stage and the water requirement is small. From the beginning of reproductive growth, the water demand is gradually increased, and if water is lacked at the moment, the growth is influenced. In the bud period, the soil must be kept moist frequently to prevent the buds from falling off due to drought, and the buds are watered for 1 time every 1 week.
3.4 separate planting
The day lily needs to be planted for 2-3 years, so that the decline caused by the over-dense plants is prevented, and the general multiplication coefficient of each day lily per year is about 1: 3. Usually, each cluster of hemerocallis fulva can keep less than 15-16 branches, and more than 20 branches of the cluster of hemerocallis fulva must be planted.
3.5 Pest control
In the hemerocallis breeding test, the snail in 5-8 months has serious harm to the hemerocallis middendorfii, and a prevention measure should be taken timely
4. Novel tissue culture
(1) Selecting flower stem as explant, washing with tap water for 30 min, sterilizing with 5% ethanol for 30s, and adding 0.1% HgCl2Soaking for 8min, sterilizing, soaking in 0.5% VC for 1 min, and transplanting to culture medium;
(2) primary culture, wherein the callus induction culture medium is MS +0.2mg/LNAA +2 mg/L6-BA;
(3) carrying out enrichment culture, wherein the adventitious bud is induced into MS +0.3mg/LNAA +1.0 mg/L6-BA;
(4) rooting culture, 1/2MS +0.2 mg/LNAA.
The culture temperature is 25 ℃, the illumination intensity is 2000lx, and the illumination time is 12 h/d. Conventional tissue culture transplantation methods of hemerocallis fulva are used for seedling exercising and transplanting.

Claims (5)

1. A method for breeding hemerocallis fulva is characterized by comprising the following steps:
(1) collecting and evaluating hemerocallis fulva germplasm resources, screening disease-resistant germplasm, and screening evergreen and disease-resistant germplasm suitable for regional climate and soil conditions;
(2) selecting and breeding a fine variety of the hemerocallis, including chromosome ploidy analysis of the hemerocallis germplasm, selecting a tetraploid large flower type or diploid small flower dwarf greening variety, then selecting evergreen, disease-resistant and heat-resistant target traits by utilizing a molecular marker technology, and finally carrying out directional breeding on a new variety of the hemerocallis through cross breeding;
(3) and carrying out plant tissue culture and propagation on the obtained variety.
2. The method for breeding and selecting hemerocallis fulva according to claim 1, comprising the following steps:
(1) selecting a parent material A of red pirate hemerocallis fulva and a parent material B of golden baby hemerocallis fulva to plant in Jianghu and Zhejiang Shanghai;
(2) selecting dominant plants in the planting area, taking flower stems of the dominant plants as explants, and carrying out tissue culture and transplanting to fields to obtain F1 generations A and B;
(3) carrying out chromosome ploidy analysis on the dominant plant, selecting red pirates with tetraploid large flower types and dolls with diploid small flower types, and selecting evergreen, disease-resistant and heat-resistant target characters by utilizing molecular markers;
(4) and (3) performing two positive and negative hybridization modes of A multiplied by B and B multiplied by A on the parent material A and the parent material B of the selected plant F1 to respectively obtain hemerocallis hybrid varieties AF1 and BF1, performing ploidy analysis and molecular marker selection again to obtain the optimal variety AF1, and performing tissue culture propagation.
3. The method for breeding and selecting hemerocallis fulva according to claim 2, wherein the tissue culture comprises the following steps:
(1) selecting flower stem as explant, washing with tap water for 30 min, sterilizing with 5% ethanol for 30s, and adding 0.1% HgCl2Soaking for 8 min;
(2) primary culture, wherein the callus induction culture medium is MS +0.2mg/LNAA +2 mg/L6-BA;
(3) carrying out enrichment culture, wherein the adventitious bud is induced into MS +0.3mg/LNAA +1.0 mg/L6-BA;
(4) rooting culture, 1/2MS +0.2 mg/LNAA.
4. The method for breeding and selecting Hemerocallis fulva according to claim 3, wherein the explants are soaked in 0.5% VC for 1 minute after sterilization and then transplanted into the culture medium.
5. The method for breeding and selecting Hemerocallis fulva according to claim 3 or 4, wherein the cultivation temperature is 25 ℃, the illumination intensity is 2000lx, and the illumination time is 12 h/d.
CN202010810861.1A 2020-08-13 2020-08-13 Breeding and breeding method for hemerocallis fulva Pending CN112167048A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010810861.1A CN112167048A (en) 2020-08-13 2020-08-13 Breeding and breeding method for hemerocallis fulva

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010810861.1A CN112167048A (en) 2020-08-13 2020-08-13 Breeding and breeding method for hemerocallis fulva

Publications (1)

Publication Number Publication Date
CN112167048A true CN112167048A (en) 2021-01-05

Family

ID=73919240

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010810861.1A Pending CN112167048A (en) 2020-08-13 2020-08-13 Breeding and breeding method for hemerocallis fulva

Country Status (1)

Country Link
CN (1) CN112167048A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116058279A (en) * 2023-01-06 2023-05-05 玉溪云星生物科技有限公司 Method for directional and efficient breeding of new variety of fragrant butterfly orchid

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102144550A (en) * 2011-01-19 2011-08-10 宁波城市职业技术学院 Tissue culture method for flower buds of hemerocallis middendorfii 'prunus lannesiana wils'
CN102172224A (en) * 2011-03-24 2011-09-07 黑龙江生态工程职业学院 Red flower orange daylily tissue culture formula and culture method
CN102197787A (en) * 2011-04-15 2011-09-28 山西省农业科学院旱地农业研究中心 Method for quickly propagating hemerocallis hybrid by culture of ovary tissues
USPP23155P2 (en) * 2011-06-21 2012-10-30 Virginia Nurserymen's Horticulture Research Foundation Inc. Hemerocallis plant named ‘VT Spirit’
CN106718864A (en) * 2017-03-01 2017-05-31 浙江省萧山棉麻研究所 A kind of method of the germplasm innovation of tawny daylily
CN106973791A (en) * 2017-04-05 2017-07-25 山西省农业科学院旱地农业研究中心 A kind of method that ethylmethane sulfonate Vitro Mutation hemerocailis middendorffi produces mutant
CN111157575A (en) * 2020-02-24 2020-05-15 上海应用技术大学 Method for screening drought-resistant hemerocallis fulva based on leaf surface temperature of hemerocallis fulva

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102144550A (en) * 2011-01-19 2011-08-10 宁波城市职业技术学院 Tissue culture method for flower buds of hemerocallis middendorfii 'prunus lannesiana wils'
CN102172224A (en) * 2011-03-24 2011-09-07 黑龙江生态工程职业学院 Red flower orange daylily tissue culture formula and culture method
CN102197787A (en) * 2011-04-15 2011-09-28 山西省农业科学院旱地农业研究中心 Method for quickly propagating hemerocallis hybrid by culture of ovary tissues
USPP23155P2 (en) * 2011-06-21 2012-10-30 Virginia Nurserymen's Horticulture Research Foundation Inc. Hemerocallis plant named ‘VT Spirit’
CN106718864A (en) * 2017-03-01 2017-05-31 浙江省萧山棉麻研究所 A kind of method of the germplasm innovation of tawny daylily
CN106973791A (en) * 2017-04-05 2017-07-25 山西省农业科学院旱地农业研究中心 A kind of method that ethylmethane sulfonate Vitro Mutation hemerocailis middendorffi produces mutant
CN111157575A (en) * 2020-02-24 2020-05-15 上海应用技术大学 Method for screening drought-resistant hemerocallis fulva based on leaf surface temperature of hemerocallis fulva

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李秀华等: ""大花萱草组培快繁体系的研究"", 《植物研究》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116058279A (en) * 2023-01-06 2023-05-05 玉溪云星生物科技有限公司 Method for directional and efficient breeding of new variety of fragrant butterfly orchid
CN116058279B (en) * 2023-01-06 2024-04-05 玉溪云星生物科技有限公司 Method for directional and efficient breeding of new variety of fragrant butterfly orchid

Similar Documents

Publication Publication Date Title
CN103371093B (en) Azalea tissue culture seedling out-bottle rooting method
CN102939898B (en) Method for catalpa bungei clonal seed selection
CN103385073A (en) Pepper overwintering cultivation method
CN103404428B (en) Cross-breeding method of Fuhong large cherry
CN112889393A (en) Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds
CN103385080A (en) Pepper spring open field culture field management method
CN104285779A (en) Method for hybridizing and breeding fast-growing cold-resistant poplar varieties
CN103947445B (en) A kind of chrysanthemum is reserved seed for planting seedling-cultivating method
CN100356839C (en) Artificial dwarfing and yield increasing jatropha cultivating process
CN105309148A (en) Petunia hybrid planting method
CN112167048A (en) Breeding and breeding method for hemerocallis fulva
CN101015254A (en) Method for cultivating ground-cover chrysanthemum blossoming twice in one year
CN103518501A (en) Geranium cultivation technique
CN1042489C (en) Parent combination process for artificially culturing paeonia suffruticosa
CN102027848B (en) All-year quick breeding technique for dahlia foot sprouts
CN103931499A (en) Tissue culture rapid propagation method for callistemon rigidus
Chen et al. Tea plant (Camellia sinensis) breeding in Korea
CN101855991B (en) Cultivation method of primula
CN112154887A (en) Cultivation and domestication method for limonium aureum
CN101011011B (en) Overwintering promoting method for clove strain 'Luolanzi' sprout
CN105494078A (en) Method using young embryo to save and obtain interspecies hybrid of hydrangea macrophylla
CN102845210B (en) Method for improving seed yield of double China aster
CN113016485B (en) Method for cultivating Jiangnan pagodatree in cold area and flourishing flowers for multiple times
CN101843216B (en) Primrose breeding method
Singh et al. CSIR-IHBT-Gr-11-6 (IC0630601; INGR20100), a Gerbera (Gerbera jasmeonii) Germplasm with Double Flower Shape

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20210105