CN101011011B - Overwintering promoting method for clove strain 'Luolanzi' sprout - Google Patents
Overwintering promoting method for clove strain 'Luolanzi' sprout Download PDFInfo
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- CN101011011B CN101011011B CN2007100636163A CN200710063616A CN101011011B CN 101011011 B CN101011011 B CN 101011011B CN 2007100636163 A CN2007100636163 A CN 2007100636163A CN 200710063616 A CN200710063616 A CN 200710063616A CN 101011011 B CN101011011 B CN 101011011B
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- 244000223014 Syzygium aromaticum Species 0.000 title claims description 33
- 235000016639 Syzygium aromaticum Nutrition 0.000 title claims description 33
- 230000001737 promoting effect Effects 0.000 title 1
- 239000004800 polyvinyl chloride Substances 0.000 claims description 10
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000002834 transmittance Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 241001104043 Syringa Species 0.000 description 17
- 235000004338 Syringa vulgaris Nutrition 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 5
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 238000009331 sowing Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 1
- 240000004307 Citrus medica Species 0.000 description 1
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- 235000002555 Citrus medica var sarcodactylis Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 239000005985 Paclobutrazol Substances 0.000 description 1
- 241000700608 Sagitta Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
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- 230000004069 differentiation Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
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- 239000005556 hormone Substances 0.000 description 1
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- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
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- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
The invention relates to a method for wintering and accelerating the growth of bluish violet seed, which comprises that putting the bluish violet into the small hut without increasing temperature at the first winter after planting the bluish violet seed. The invention can hold best thermal and water conditions, to prolong the growth period.
Description
Technical field
The present invention relates to the short rectangular method of surviving the winter of plant seedlings, particularly relate to the short rectangular method of surviving the winter of cloves seedling, especially relate to clove strain ' the short rectangular method of surviving the winter of seedling.
Background technology
Clove strain ' be that the early lilac flower that the Institute of Botany, Chinese Academy of Sciences botanical garden utilizes China to originate in is made female parent, make male parent with European lilac (syringa) cultivar ' Buddha's hand cloves ', by the lilac (syringa) cultivar of artificial hybridization cultivation.In the breeding owing to adopted the extremely strong native country of resistance primary cloves germplasm early lilac flower, and the stronger polyphyll Europe lilac (syringa) cultivar of sight is a hybrid strain, make the gained strain ' have not only that pattern is dense, the flower type is plentiful, inflorescence is compact, the very excellent fancy points such as intensive of blossoming, compare with the external lilac (syringa) cultivar of introducing in recent years simultaneously, have more outstanding heat resistance, cold resistant, drought-resistant barren resistance feature, in northern city and regional vegetation planting, can effectively reduce the gardens maintenance cost.This kind 1996 is by the registration of international cloves association; Calendar year 2001 obtains the 5th Chinese flower fair gold medal; The year ends 2005, this kind was passed through State Administration of Forestry's forest breeding authorization.In provinces such as China Inner Mongol, Liaoning, Heilungkiang cultivation is arranged at present more.
Comparatively regular vegetative propagation has the history in over one hundred year so far at lilac (syringa) cultivar, no matter is that cloves fan or professional staff's methods of graftings, plant division, cuttage that adopt in the breeding of lilac (syringa) cultivar are carried out (Mickey1928 more; Cameron et al.2003).Say objectively,, restricting the proliferate efficiency of these genus improved seeds for a long time all the time owing to the particularity of genus syringa endogenous hormones and secondary meristem differentiation.In 20 end of the centurys, cultivating idol for the bud explant of European cloves has report (Refouvelet et al.1998).In addition and since the tissue culture of this class woody plant no matter the bottle internal breeding, still bottle outlet transplant and the accumulation of outdoor cropping amount of growth slowly, the restriction of factor such as the virgin phase of seedling is long, utilize extensive not enough that the work of tissue culture quick breeding carries out.It seems at present,, adopt the bud explant inoculation more safer in the tissue-culturing rapid propagation than the callus induction of other explant in order to keep the original excellent proterties of target variety.Therefore, be purpose unless induce with variation, the fast numerous explant of current lilac (syringa) cultivar is common relatively with bud explant.But in the process of utilization tissue-culturing quick-propagation cloves seedling, plant the back amount of growth to hang down into seedling slow tissue cultivating seedling, and the virgin phase is prolonged, and makes to cultivate time limit increase and influence the commercialization process to a certain extent.
Summary of the invention
The purpose of this invention is to provide a kind of clove strain ' the short rectangular method of surviving the winter of seedling.
In first winter after clove strain provided by the present invention ' seedling the short rectangular method of surviving the winter be ' the seedling field planting, be placed in the hut that adds button in the booth of not heating and survive the winter at clove strain.
Described clove strain ' seedling is the seedling that tissue culture obtains.
The light transmittance of described booth is 80-90%; Be preferably 90%.
The light transmittance of described hut is 80-90%.
Described clove strain ' group training seedling time of surviving the winter in hut is 1 year 3 the end of month of field planting first arrival in October then.
The material of described hut can be polyvinyl chloride does not have the film or the common polyvinyl chloride horticultural covering film of dripping.
The material of described booth can be polyvinyl chloride does not have the film or the common polyvinyl chloride horticultural covering film of dripping.
Method of the present invention can keep the best hydro-thermal situation of subenvironment, is equivalent to prolong vegetative period, has promoted building up in early days of biomass, and is still keeping growth vigor for many years after this, remarkable effect is arranged for the virgin phase that shortens woody tissue cultivating seedling.
Description of drawings
Fig. 1 adds cloves group training seedling that the cloves group training seedling of detaining hut and fallen leaves the cover upgrowth situation photo of surviving the winter
Fig. 2 handles the back and does not add the main environment parameter difference of detaining hut for adding the button hut in the booth of not heating winter
Fig. 3 adds the button hut in the booth to be handled the back and not to add the button hut and survive the winter and handle the biomass difference of seedling after after the field planting 2 years for not heating winter.
Embodiment
Method among the following embodiment if no special instructions, is conventional method.
The short rectangular method of surviving the winter of embodiment: clove strain ' seedling
1, clove strain ' acquisition of group training seedling
1) explant collection and sterilization
Explant is gathered: gather clove strain ' giving birth to young sprout then, to serve as a fill-in 4-6 be explant to lateral bud.
Sterilization: with adopting clove strain ' to be immersed in volumn concentration be that 20-30 carries out surface sterilization second in 70% alcoholic solution to lateral bud; Add 3 soil temperatures in the clorox of quality percentage composition 5%, mix, subsequently the bud input was soaked 6-10 minute, stir simultaneously; Then bud is dropped into the quality percentage composition and be to stir in 0.1% the mercuric chloride solution and soaked 8-12 minute, use aseptic water washing 4-6 time at last.
2) young sprout proliferation-inducing and culture of rootage
The acquisition of stem with bud: at first above-mentioned bud explant through sterilization is seeded in the blank medium of MS, at 20 ℃-23 ℃, air humidity is lower than under 50% the condition cultivates, treat bud sprouting extraction young sprout, young sprout is grown to 1.5-3cm long, after having 2-3 to lateral bud, it is cut into the tender stem section that has newborn lateral bud, i.e. stem with bud.
With the above-mentioned clove strain that obtains ' the tender stem section (stem with bud) that has newborn lateral bud, in the medium that contains 1.0mg/L BAP and 0.1mg/L ZT, carry out the young sprout proliferation-inducing and be cultured to 2-3 after week, young sprout with propagation carries out culture of rootage in the 1/2MS medium that contains 1.0mg/L IAA+1.0mg/L paclobutrazol again, cultivates the bottle seedling that obtains taking root after 2 weeks (be clove strain ' seedling).
2, seedling is obtained in cuttage
Get clove strain in the season of growth ' semi-lignified branch, be cut into plugged ear at nearly bud place, dipping in and get that root-inducing powder inserts with the coarse sand under relative moisture 80%, temperature 25-28 ℃ condition is the slotting machine of matrix, and month remaining years root obtains clove strain ' seedling.
3, directly sowing obtains seedling
Get the healthy clove strain of the previous year ' seed directly sowing at the beginning of 4 months, obtain healthy and strong clove strain ' seedling
4, clove strain ' seedling survive the winter short long
With step 1 obtain take root after the seedling that obtains of the cuttage that obtains of bottle seedling, step 2 or the seedling that obtains of the direct sowing that obtains of step 3, after being transplanted to the land for growing field crops respectively, first winter (late October-March in next year) is at the booth of not heating (the common polyvinyl chloride horticultural covering film of its plantation, light transmittance is 80%) in implement to add button little plastic greenhouse (common polyvinyl chloride horticultural covering film, light transmittance are 80%).Hut supports with iron shed, 0.6 meter of sagitta, and 1 meter of span, long 2 meters, the sealing of the 800 strain seedling of planting approximately in each hut is handled, to cover seedling in contrast with fallen leaves in the same booth of not heating.
In year March in October-second, monitored the temp. and humidity of growth of seedling environment (booth or hut) in every month, humidity is read by recording clock, temperature is obtained with artificial reading, get at 9 in the morning and afternoon 2 data analyze, concrete data are as shown in Figure 2, the result shows, add the button hut temperature in the subenvironment was obviously increased in whole winter, particularly make temperature maintenance in the subenvironment near 25 ℃ level, promptly be equivalent to prolonged one month vegetative period in October.Approaching saturated relative moisture in the subenvironment helps to keep the optimum moisture situation of seedling simultaneously.
At the end of 1 year three months, open hut, observe the growth situation of the seedling that passes the winter.The result shows that leaf area and plant height are significantly higher than the processing that does not add hut behind the button hut.Survival rate of seedling is about 80%, and fallen leaves cover the seedling percent pass the winter and only have 30%, and branches and leaves degree sturdy and in great numbers and growing way are all obviously than not adding the button hut.The survival rate that the seedling that bottle seedling after taking root, the seedling that cuttage obtains or direct sowing obtain passes the winter in adding the button hut is respectively 80%, 100%, 100%; In booth, cover the survival rate of seedling of passing the winter and be respectively 30%, 80%, 90% with fallen leaves.Wherein, the bottle seedling that obtains of tissue culture adds the photo of passing the winter with booth decline foliage cover in the button hut as shown in Figure 1 in booth.
When opening hut at the end of 1 year three months, measurement adds the button hut, reaches the biomass (A among Fig. 3) that fallen leaves cover the seedling that passes the winter, and after 2 years, carries out same biomass detection (B among Fig. 3) with the seedling open country field planting that is about to two kinds of processing of surviving the winter again.The result shows that the tissue-culture container seedling of surviving the winter in 1 year behind the intermediate house does not cover the growth of seedling amount height of surviving the winter than not adding the button hut then with fallen leaves, under the combination of the suitableeest temp. and humidity, biomass is accumulated fast in a seedling stage in age in adding the button hut.By the comparison for many years of this processing observation is found, can keep for many years in the remarkable build-up effect of seedling stage in an age biomass, the tissue cultivating seedling quality is significantly improved.Tissue-culture container seedling is survived the winter in the hut and is not covered 1 year of surviving the winter and survive the winter the artifact amount relatively shown in A among Fig. 3 with not adding the hut fallen leaves adding button, and the biomass after tissue-culture container seedling was survived the winter back 2 years is relatively shown in B among Fig. 3.
Claims (5)
1. in first winter after clove strain ' seedling the short rectangular method of surviving the winter, be ' seedling field planting, be placed in the hut that adds button in the booth of not heating and survive the winter at clove strain;
Described clove strain ' seedling is the seedling that tissue culture obtains;
Described clove strain ' to cultivate the time of surviving the winter in hut be 1 year 3 the end of month of field planting first arrival in October then to seedling.
2. method according to claim 1 is characterized in that: the light transmittance of described booth is 80-90%.
3. method according to claim 1 is characterized in that: the light transmittance of described hut is 80-90%.
4. method according to claim 3 is characterized in that: the material of described hut is that polyvinyl chloride does not have the film or the common polyvinyl chloride of dripping.
5. method according to claim 4 is characterized in that: the material of described booth is that polyvinyl chloride does not have the film or the common polyvinyl chloride of dripping.
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| CN2007100636163A CN101011011B (en) | 2007-02-06 | 2007-02-06 | Overwintering promoting method for clove strain 'Luolanzi' sprout |
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| CN2007100636163A CN101011011B (en) | 2007-02-06 | 2007-02-06 | Overwintering promoting method for clove strain 'Luolanzi' sprout |
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| CN104705062A (en) * | 2015-03-27 | 2015-06-17 | 陈苏依勒 | Alxa agilawood planting method |
| CN105594573B (en) * | 2016-03-02 | 2018-02-06 | 云南省农业科学院花卉研究所 | A kind of method for shortening camellia commodity potted flower juvenile phase |
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| CN2682809Y (en) * | 2003-08-22 | 2005-03-09 | 郝麟文 | Regulate and control greenhouse |
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| CN2682809Y (en) * | 2003-08-22 | 2005-03-09 | 郝麟文 | Regulate and control greenhouse |
Non-Patent Citations (2)
| Title |
|---|
| 刘建斌等.紫丁香的组织培养.北京农学院学报16 4.2001,16(4),42-44. |
| 刘建斌等.紫丁香的组织培养.北京农学院学报16 4.2001,16(4),42-44. * |
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