CN112142514A - Microbial fertilizer for promoting decomposition of soil organic matters and preparation process thereof - Google Patents
Microbial fertilizer for promoting decomposition of soil organic matters and preparation process thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
Abstract
The invention discloses a microbial fertilizer for promoting decomposition of soil organic matters, which comprises diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder, lignin grafted polyacrylonitrile, a microbial agent, cyanobacteria liquid and trace elements, wherein the microbial inoculant comprises bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis and aspergillus niger. The microbial fertilizer in the embodiment of the invention can effectively increase the yield of the balsam pear planted in the saline-alkali soil; the microbial fertilizer can effectively reduce the pH value of the soil and the salt content and organic matter content in the soil. The preparation method of the microbial fertilizer is simple, the related microbial strains are common strains on the market, the use method is simple, the microbial fertilizer does not conflict with conventional fertilization, the yield increasing effect and the soil physical property improving effect are obvious, and the microbial fertilizer is recommended to be popularized and used in saline-alkali soil areas.
Description
Technical Field
The invention relates to the technical field of soil organic matter degradation, in particular to a microbial fertilizer for promoting soil organic matter decomposition and a preparation process thereof.
Background
The soil organic matter is an important component of soil, and has important effects on soil fertility and ecological environment, and the soil organic matter refers to all carbon-containing organic substances existing in the soil, including various animals, plant residues, microorganisms and various decomposed and synthesized organic substances in the soil. After being artificially influenced, the natural soil also comprises organic substances such as organic fertilizers, industrial, agricultural and domestic wastewater, waste residues, microbial products, organic pesticides and the like. The decomposition of organic matters in soil mainly depends on microorganisms in the soil, but in many cultivated lands in China, especially saline-alkali lands, due to the fact that the types of the microorganisms in the soil are few and the microorganisms in the soil are active, the decomposition rate of the organic matters is slow, the effectiveness of nutrients is low, and further the crop yield on the lands is low.
Disclosure of Invention
In order to solve the technical problems, the invention provides a microbial fertilizer for promoting the decomposition of soil organic matters, which comprises the following components in parts by mass: 15-28 parts of diatomite, 8-12 parts of bamboo powder, 3-5 parts of lentinan, 3-5 parts of yeast extract, 2-8 parts of vitamin C powder, 2-6 parts of lignin grafted polyacrylonitrile, 3-7 parts of a microbial agent, 4-6 parts of cyanobacterial liquid and 3-7 parts of trace elements.
As an alternative of the technical scheme of the invention, the microbial agent is a solid microbial agent, and comprises the following components in parts by mass: 15-22 parts of bacillus thuringiensis, 8-12 parts of bacillus subtilis, 10-16 parts of bacillus megaterium, 20-30 parts of bacillus mucilaginosus, 8-16 parts of bacillus laterosporus, 5-13 parts of streptomyces jingyangensis and 7-11 parts of aspergillus niger.
As an alternative of the technical scheme of the invention, the microbial agent is a solid microbial agent, and comprises the following components in parts by mass: 18-20 parts of bacillus thuringiensis, 10-12 parts of bacillus subtilis, 12-14 parts of bacillus megaterium, 24-28 parts of bacillus mucilaginosus, 10-12 parts of bacillus laterosporus, 8-11 parts of streptomyces jingyangensis and 9-10 parts of aspergillus niger.
As an alternative of the technical scheme of the invention, the microbial agent is a solid microbial agent, and comprises the following components in parts by mass: bacillus thuringiensis 19, bacillus subtilis 11, bacillus megaterium 13, bacillus mucilaginosus 26, bacillus laterosporus 11, streptomyces jingyangensis 10 and aspergillus niger 9.5.
As an alternative scheme of the technical scheme of the invention, the trace elements comprise magnesium sulfate, manganese sulfate and zinc sulfate, and the mass ratio of the magnesium sulfate to the zinc sulfate is (3): manganese sulfate 3: and 1, zinc sulfate.
The invention also provides a preparation process of the microbial fertilizer for promoting the decomposition of soil organic matters, which is characterized by comprising the following steps of,
s1, activating and acclimating bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis and aspergillus niger on corresponding culture media respectively to prepare respective powder, and then uniformly mixing all strains according to the mass ratio to obtain a microbial agent for later use;
s2, uniformly mixing magnesium sulfate, manganese sulfate and zinc sulfate according to the mass ratio of 3:3:1 respectively to prepare trace elements, and mixing the trace elements with the microbial agent prepared in S1;
s3, mixing diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder and lignin grafted polyacrylonitrile according to the mass ratio, and wrapping the mixture outside a microbial agent and trace element mixture by using a granulator;
s4, weighing 1.5g NaNO in a clean triangular flask3，0.04gK2HPO4，0.075gMgSO4·7H2O，0.036gCaCl2·7H2O，0.02gNa2CO30.006g of citric acid, 0.006g of ferric citrate, 1ml of A5 solution, ampicillin with a final concentration of 50 mu g/ml, and 20.0g of agar which are made into 1000ml by distilled water;
wherein the A5 solution is 2.86gH3BO4，1.81gMnCl2·4H2O，0.222gZnSO4，0.39gNa2MoO4，0.079gCuSO4·5H2O，49.4gCo(NO3)2·6H2Adding distilled water into the mixture of O, fixing the volume to 1000ml, inoculating cyanobacteria into the triangular flask according to the inoculation amount of 5% and culturing for 3d to obtain cyanobacteria liquid;
and S5, spraying the cyanobacteria liquid outside the product in the S3, and air-drying to obtain the microbial fertilizer for promoting the decomposition of soil organic matters.
As a technical solution of the present inventionAlternatively, the effective viable count of the microorganisms in each microbial powder in step S1 is Bacillus thuringiensis 107～108CFU/g, Bacillus subtilis 3X 106～7×107CFU/g, Bacillus megaterium 8X 106～6×107CFU/g, Bacillus mucilaginosus 105～3×106CFU/g, Bacillus laterosporus 4X 106～2×107CFU/g, 8X 10 Streptomyces jingyangensis5～4×106CFU/g, Aspergillus niger 106～107CFU/g。
As an alternative of the technical scheme of the invention, in step S4, the effective viable count of the cyanobacteria in the cyanobacteria liquid is (1-8) x 105CFU/ml。
The invention has the beneficial effects that:
compared with the conventional means, the microbial fertilizer in the embodiment of the invention can effectively increase the single-plant fruit number, the single-fruit length and the single-fruit weight of the bitter gourds planted in the saline-alkali soil, thereby improving the acre yield of the bitter gourds in the saline-alkali soil; by analyzing the physical and chemical properties of the soil, the microbial fertilizer can effectively reduce the pH value of the soil and the salt content and organic matter content in the soil. The preparation method of the microbial fertilizer is simple, the related microbial strains are common strains on the market, the use method is simple, the microbial fertilizer does not conflict with conventional fertilization, the yield increasing effect and the soil physical property improving effect are obvious, and the microbial fertilizer is recommended to be popularized and used in saline-alkali soil areas.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the case of the example 1, the following examples are given,
a microbial fertilizer for promoting decomposition of soil organic matters comprises the following components in parts by mass: 15 parts of diatomite, 8 parts of bamboo powder, 3 parts of lentinan, 5 parts of yeast extract, 8 parts of vitamin C powder, 6 parts of lignin grafted polyacrylonitrile, 7 parts of microbial agent, 4 parts of cyanobacteria liquid and 7 parts of trace elements. The microbial agent is a solid microbial agent and comprises the following components in parts by mass: bacillus thuringiensis 19, bacillus subtilis 11, bacillus megaterium 13, bacillus mucilaginosus 26, bacillus laterosporus 11, streptomyces jingyangensis 10 and aspergillus niger 9.5. The trace elements comprise magnesium sulfate, manganese sulfate and zinc sulfate, and the mass ratio of the magnesium sulfate to the zinc sulfate is magnesium sulfate 3: manganese sulfate 3: and 1, zinc sulfate.
Wherein the effective viable count of the microorganisms in each microbial powder is Bacillus thuringiensis 107CFU/g, Bacillus subtilis 3X 106CFU/g, Bacillus megaterium 8X 106CFU/g, Bacillus mucilaginosus 105CFU/g, Bacillus laterosporus 4X 106CFU/g, 8X 10 Streptomyces jingyangensis5CFU/g, Aspergillus niger 106CFU/g; the effective viable count of cyanobacteria in the cyanobacteria solution is 2 × 105CFU/ml。
In the case of the example 2, the following examples are given,
a microbial fertilizer for promoting decomposition of soil organic matters comprises the following components in parts by mass: 28 parts of diatomite, 12 parts of bamboo powder, 5 parts of lentinan, 5 parts of yeast extract, 2 parts of vitamin C powder, 2 parts of lignin grafted polyacrylonitrile, 3 parts of microbial agent, 4 parts of cyanobacteria liquid and 3 parts of trace elements. The microbial agent is a solid microbial agent and comprises the following components in parts by mass: bacillus thuringiensis 18, bacillus subtilis 10, bacillus megaterium 12, bacillus mucilaginosus 28, bacillus laterosporus 12, streptomyces jingyangensis 11 and aspergillus niger 10. The trace elements comprise magnesium sulfate, manganese sulfate and zinc sulfate, and the mass ratio of the magnesium sulfate to the zinc sulfate is magnesium sulfate 3: manganese sulfate 3: and 1, zinc sulfate.
Wherein the effective viable count of the microorganisms in each microbial powder is 5 multiplied by 10 of bacillus thuringiensis7CFU/g, Bacillus subtilis 8X 106CFU/g, Bacillus megaterium 3X 107CFU/g, Bacillus mucilaginosus 5X 105CFU/g, Bacillus laterosporus 8X 106CFU/g, Streptomyces jingyangensis 2X 106CFU/g, Aspergillus niger 4X 106CFU/g; the effective viable count of cyanobacteria in the cyanobacteria solution is 4 × 105CFU/ml。
In the case of the example 3, the following examples are given,
a microbial fertilizer for promoting decomposition of soil organic matters comprises the following components in parts by mass: 20 parts of diatomite, 20 parts of bamboo powder, 4 parts of lentinan, 4 parts of yeast extract, 5 parts of vitamin C powder, 4 parts of lignin grafted polyacrylonitrile, 5 parts of microbial agent, 5 parts of cyanobacterial liquid and 5 parts of trace elements. The microbial agent is a solid microbial agent and comprises the following components in parts by mass: bacillus thuringiensis 20, bacillus subtilis 12, bacillus megaterium 14, bacillus mucilaginosus 24, bacillus laterosporus 10, streptomyces jingyangensis 8 and aspergillus niger 9. The trace elements comprise magnesium sulfate, manganese sulfate and zinc sulfate, and the mass ratio of the magnesium sulfate to the zinc sulfate is magnesium sulfate 3: manganese sulfate 3: and 1, zinc sulfate.
Wherein the effective viable count of the microorganisms in each microbial powder is 5 multiplied by 10 of bacillus thuringiensis7CFU/g, Bacillus subtilis 8X 106CFU/g, Bacillus megaterium 3X 107CFU/g, Bacillus mucilaginosus 5X 105CFU/g, Bacillus laterosporus 8X 106CFU/g, Streptomyces jingyangensis 2X 106CFU/g, Aspergillus niger 4X 106CFU/g; the effective viable count of cyanobacteria in the cyanobacteria solution is 4 × 105CFU/ml。
It should be noted that, the inventors have verified the antagonistic properties of bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis, aspergillus niger and cyanobacteria involved in the above three embodiments, and no antagonistic phenomenon exists between every two of the three embodiments.
In the case of the example 4, the following examples are given,
the embodiment provides the preparation process of the microbial fertilizer for promoting the decomposition of soil organic matters in the embodiments 1 to 3, which comprises the following steps,
s1, activating and acclimating bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis and aspergillus niger on corresponding culture media respectively to prepare respective powder, and then uniformly mixing all strains according to the mass ratio to obtain a microbial agent for later use;
s2, uniformly mixing magnesium sulfate, manganese sulfate and zinc sulfate according to the mass ratio of 3:3:1 respectively to prepare trace elements, and mixing the trace elements with the microbial agent prepared in S1;
s3, mixing diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder and lignin grafted polyacrylonitrile according to the mass ratio, and wrapping the mixture outside a microbial agent and trace element mixture by using a granulator;
s4, weighing 1.5g NaNO in a clean triangular flask3，0.04gK2HPO4，0.075gMgSO4·7H2O，0.036gCaCl2·7H2O，0.02gNa2CO30.006g of citric acid, 0.006g of ferric citrate, 1ml of A5 solution, ampicillin with a final concentration of 50 mu g/ml, and 20.0g of agar which are made into 1000ml by distilled water; wherein the A5 solution is 2.86gH3BO4，1.81gMnCl2·4H2O，0.222gZnSO4，0.39gNa2MoO4，0.079gCuSO4·5H2O，49.4gCo(NO3)2·6H2Adding distilled water into the mixture of O, fixing the volume to 1000ml, inoculating cyanobacteria into the triangular flask according to the inoculation amount of 5% and culturing for 3d to obtain cyanobacteria liquid;
and S5, spraying the cyanobacteria liquid outside the product in the S3, and air-drying to obtain the microbial fertilizer for promoting the decomposition of soil organic matters.
In order to verify the effect of the microbial fertilizer in promoting the decomposition of organic matters in saline-alkali soil, the following comparative examples are also designed:
in the comparative example 1,
a contrast fertilizer for a microbial fertilizer for promoting decomposition of soil organic matters comprises the following components in parts by mass: 20 parts of diatomite, 20 parts of bamboo powder, 4 parts of lentinan, 4 parts of yeast extract, 5 parts of vitamin C powder, 4 parts of lignin grafted polyacrylonitrile, 5 parts of cyanobacteria liquid and 5 parts of trace elements. The trace elements comprise magnesium sulfate, manganese sulfate and zinc sulfate, and the mass ratio of the magnesium sulfate to the zinc sulfate is magnesium sulfate 3: manganese sulfate 3: and 1, zinc sulfate.
The preparation method of the comparative fertilizer comprises the following steps: s1, uniformly mixing magnesium sulfate, manganese sulfate and zinc sulfate according to the mass ratio of 3:3:1 respectively to prepare trace elements;
s2, mixing diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder and lignin grafted polyacrylonitrile according to the mass ratio, and then wrapping the mixture outside the trace element mixture by using a granulator;
the application case is as follows:
1. test materials and methods
1.1 test crops and fertilizers
The balsam pear variety is Haiyang No. 2, and is planted in 5 months; the fertilizers comprise the microbial fertilizer, the decomposed organic fertilizer, phosphorus pentoxide, potassium sulfate and urea disclosed in the embodiments 1-3 of the invention.
1.2 design of the experiment
The test is provided with 5 treatments in total, each treatment is repeated for 3 times, 15 test cells are in total, all the cells are randomly arranged, and protection rows are arranged among the cells and around the test cells.
Treatment 1: conventional fertilization + microbial fertilizer of example 1 of the invention
And (3) treatment 2: conventional fertilization + microbial fertilizer of embodiment 2 of the invention
And (3) treatment: conventional fertilization + microbial fertilizer of embodiment 3 of the invention
And (4) treatment: conventional fertilization + comparative fertilizer of comparative example 1 of the invention
And (4) treatment 5: conventional fertilization
1.3 test soil Condition
A cell test for applying the microbial fertilizer to the balsam pear is carried out in a saline-alkali land of the Longshan town of the coastal economic development area of Weifang city, and the influence of the microbial fertilizer on the decomposition of organic matters in soil and the yield increasing effect of crops are verified.
The method comprises the steps of firstly detecting the pH value of soil, the salt content of the soil and the water content of the soil in 15 test areas which are processed in total by 5 treatment, sampling each test area according to a 5-point diagonal method, taking a soil surface layer (0-30 cm) sample by using a soil auger, mixing the soil collected in each group into a soil sample, removing impurities such as animal and plant residues from the sample, removing redundant soil samples by a quartering method, air-drying, grinding and screening the sample, bagging for later use, and testing the parameters of physical and chemical analysis of the soil according to soil agricultural chemical analysis (third edition) compiled by Boudoudan.
In addition, a soil organic matter tester is adopted to detect the content of organic matters in the four groups of soil.
The values detected for each of the 3 test zones treated were calculated and averaged. The basic soil texture of each treatment area is shown in Table 1.
TABLE 1 basic soil texture of test area
1.4 fertilization protocol
And (3) conventional fertilization: 1) base fertilizer: the decomposed organic fertilizer is 1500 kg/mu, and the depth of the deeply ploughed soil is 10cm after the application is finished;
and (3) seedling extraction and fertilizer application: when the seedlings grow to 20-30 cm, applying 10 kg/mu of urea, 6 kg/mu of phosphorus pentoxide and 6 kg/mu of potassium sulfate;
topdressing: after one to two times of balsam pear harvesting, nitrogen fertilizer is applied once along with water, and 25 kg/mu of urea is applied.
The microbial fertilizer in the embodiments 1-3 of the invention and the application method of the comparative fertilizer in the comparative example 1 of the invention are as follows: the dosage is 15 kg/mu, and the application is carried out when balsam pear is planted.
1.5 other: the management measures such as watering, fertilizing, pest control and the like in all the areas are kept consistent.
2. Test results and analysis
2.1 Effect of different treatments on the bitter gourd fruit
In the process of harvesting, the number of the single plant results, the fruit length and the fruit weight of each single balsam pear are counted, and the counting results and the analysis results are summarized in table 2.
TABLE 2 balsam pear result quality statistics and analysis
As can be seen from the data in Table 2, the microbial fertilizer of the present invention in examples 1-3 can effectively increase the number of fruits per plant, the length of fruits per fruit and the weight of fruits per fruit of Momordica charantia.
2.2 Effect of different treatments on saline-alkali soil texture
Soil quality of the same area before the balsam pear planting in the second year is detected according to the method in 1.3, and the detection results are summarized in table 3.
TABLE 3 basic soil texture condition of test area-2
From the above data, it can be seen that the pH of the soil, the salt content and the organic matter content in the soil can be effectively reduced by using the microbial fertilizer in the embodiments 1 to 3 of the present invention.
3. Conclusion of the experiment
Experiments show that compared with the conventional means, the microbial fertilizer provided by the embodiment of the invention can effectively increase the single-plant fruit number, the single-fruit length and the single-fruit weight of the bitter gourds planted in the saline-alkali soil, so that the acre yield of the bitter gourds in the saline-alkali soil can be increased; by analyzing the physical and chemical properties of the soil, the microbial fertilizer can effectively reduce the pH value of the soil and the salt content and organic matter content in the soil. The preparation method of the microbial fertilizer is simple, the related microbial strains are common strains on the market, the use method is simple, the microbial fertilizer does not conflict with conventional fertilization, the yield increasing effect and the soil physical property improving effect are obvious, and the microbial fertilizer is recommended to be popularized and used in saline-alkali soil areas.
The present invention is not limited to the above-described preferred embodiments, but rather, the present invention is to be construed broadly and cover all modifications, equivalents, and improvements falling within the spirit and scope of the present invention. In addition, the technical solutions between the various embodiments can be combined with each other, but must be based on the realization of those skilled in the art; where combinations of features are mutually inconsistent or impractical, such combinations should not be considered as being absent and not within the scope of the claimed invention.
Claims (8)
1. The microbial fertilizer for promoting decomposition of soil organic matters is characterized by comprising the following components in parts by mass: 15-28 parts of diatomite, 8-12 parts of bamboo powder, 3-5 parts of lentinan, 3-5 parts of yeast extract, 2-8 parts of vitamin C powder, 2-6 parts of lignin grafted polyacrylonitrile, 3-7 parts of a microbial agent, 4-6 parts of cyanobacterial liquid and 3-7 parts of trace elements.
2. The microbial fertilizer for promoting decomposition of organic matters in soil according to claim 1, wherein the microbial agent is a solid microbial agent and comprises the following components in parts by mass: 15-22 parts of bacillus thuringiensis, 8-12 parts of bacillus subtilis, 10-16 parts of bacillus megaterium, 20-30 parts of bacillus mucilaginosus, 8-16 parts of bacillus laterosporus, 5-13 parts of streptomyces jingyangensis and 7-11 parts of aspergillus niger.
3. The microbial fertilizer for promoting decomposition of organic matters in soil according to claim 1, wherein the microbial agent is a solid microbial agent and comprises the following components in parts by mass: 18-20 parts of bacillus thuringiensis, 10-12 parts of bacillus subtilis, 12-14 parts of bacillus megaterium, 24-28 parts of bacillus mucilaginosus, 10-12 parts of bacillus laterosporus, 8-11 parts of streptomyces jingyangensis and 9-10 parts of aspergillus niger.
4. The microbial fertilizer for promoting decomposition of organic matters in soil according to claim 1, wherein the microbial agent is a solid microbial agent and comprises the following components in parts by mass: bacillus thuringiensis 19, bacillus subtilis 11, bacillus megaterium 13, bacillus mucilaginosus 26, bacillus laterosporus 11, streptomyces jingyangensis 10 and aspergillus niger 9.5.
5. The microbial fertilizer for promoting decomposition of organic matters in soil according to claim 1, wherein the trace elements comprise magnesium sulfate, manganese sulfate and zinc sulfate, and the mass ratio of the magnesium sulfate to the zinc sulfate is magnesium sulfate 3: manganese sulfate 3: and 1, zinc sulfate.
6. A preparation process of a microbial fertilizer for promoting the decomposition of soil organic matters is characterized by comprising the following steps,
s1, activating and acclimating bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis and aspergillus niger on corresponding culture media respectively to prepare respective powder, and then uniformly mixing all strains according to the mass ratio to obtain a microbial agent for later use;
s2, uniformly mixing magnesium sulfate, manganese sulfate and zinc sulfate according to the mass ratio of 3:3:1 respectively to prepare trace elements, and mixing the trace elements with the microbial agent prepared in S1;
s3, mixing diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder and lignin grafted polyacrylonitrile according to the mass ratio, and wrapping the mixture outside a microbial agent and trace element mixture by using a granulator;
s4, weighing 1.5g NaNO in a clean triangular flask3，0.04gK2HPO4，0.075gMgSO4·7H2O，0.036gCaCl2·7H2O，0.02gNa2CO30.006g of citric acid, 0.006g of ferric citrate, 1ml of A5 solution, ampicillin with a final concentration of 50 mu g/ml, and 20.0g of agar which are made into 1000ml by distilled water;
wherein the A5 solution is 2.86gH3BO4，1.81gMnCl2·4H2O，0.222gZnSO4，0.39gNa2MoO4，0.079gCuSO4·5H2O，49.4gCo(NO3)2·6H2Adding distilled water into the mixture of O, fixing the volume to 1000ml, inoculating cyanobacteria into the triangular flask according to the inoculation amount of 5% and culturing for 3d to obtain cyanobacteria liquid;
and S5, spraying the cyanobacteria liquid outside the product in the S3, and air-drying to obtain the microbial fertilizer for promoting the decomposition of soil organic matters.
7. According toThe process for preparing microbial fertilizer for promoting decomposition of organic matters in soil as claimed in claim 5, wherein the effective viable count of microorganisms in each microbial powder in step S1 is Bacillus thuringiensis 107～108CFU/g, Bacillus subtilis 3X 106～7×107CFU/g, Bacillus megaterium 8X 106～6×107CFU/g, Bacillus mucilaginosus 105～3×106CFU/g, Bacillus laterosporus 4X 106～2×107CFU/g, 8X 10 Streptomyces jingyangensis5～4×106CFU/g, Aspergillus niger 106～107CFU/g。
8. The process for preparing a microbial fertilizer for promoting decomposition of organic matters in soil according to claim 5, wherein in step S4, the effective viable count of cyanobacteria in the cyanobacteria solution is (1-8) x 105CFU/ml。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114394868A (en) * | 2022-01-21 | 2022-04-26 | 水鹏程 | Controlled-release microbial fertilizer containing polyglutamic acid |
| CN114931057A (en) * | 2022-06-16 | 2022-08-23 | 丽江市林业科学研究所 | Method for improving olive cultivation soil in Jinsha river valley region |
| CN116103204A (en) * | 2023-02-28 | 2023-05-12 | 山东中新农业发展有限公司 | Microbial decomposing inoculant for organic compost and preparation method thereof |
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2020
- 2020-09-27 CN CN202011035161.6A patent/CN112142514A/en not_active Withdrawn
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114394868A (en) * | 2022-01-21 | 2022-04-26 | 水鹏程 | Controlled-release microbial fertilizer containing polyglutamic acid |
| CN114931057A (en) * | 2022-06-16 | 2022-08-23 | 丽江市林业科学研究所 | Method for improving olive cultivation soil in Jinsha river valley region |
| CN116103204A (en) * | 2023-02-28 | 2023-05-12 | 山东中新农业发展有限公司 | Microbial decomposing inoculant for organic compost and preparation method thereof |
| CN116103204B (en) * | 2023-02-28 | 2024-02-23 | 山东中新农业发展有限公司 | Microbial decomposing inoculant for organic compost and preparation method thereof |
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