CN112125964B - 与植物粒重相关蛋白GmJAZ3及其编码基因和应用 - Google Patents
与植物粒重相关蛋白GmJAZ3及其编码基因和应用 Download PDFInfo
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- CN112125964B CN112125964B CN201910558178.0A CN201910558178A CN112125964B CN 112125964 B CN112125964 B CN 112125964B CN 201910558178 A CN201910558178 A CN 201910558178A CN 112125964 B CN112125964 B CN 112125964B
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Abstract
本发明公开了与植物粒重相关蛋白GmJAZ3及其编码基因和应用。本发明首先提供了GmJAZ3在调控植物粒重中的应用,GmJAZ3如下任一所示蛋白质:(A1)由序列2所示的氨基酸序列组成的蛋白质;(A2)序列2所示的蛋白质的N端或/和C端连接标签得到的融合蛋白;(A3)将序列2所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加得到的与A1)所示的蛋白质具有90%以上的同一性且功能相同的蛋白质。本发明GmJAZ3及其编码基因可提高植物的粒重,增加植物产量,用于培育高产品种。
Description
技术领域
本发明属于生物技术领域,具体涉及与植物粒重相关蛋白GmJAZ3及其编码基因和应用。
背景技术
大豆是重要传统作物,蕴含丰富营养价值,是提供粮油和饲料的重要经济作物,在工业生产中大豆油也有很多用途,例如生物燃料,表面活性剂,软化剂等。中国曾是世界最大的大豆生产国,然而,近年来,大豆生产远远落后于国内其他粮食作物发展的步伐,不能满足国民需要。因此提高大豆单产已成为目前亟待解决的问题。
种子的重量(粒重)是作物生产中的一个重要指标,是影响作物产量的重要农艺性状之一。植物可以通过增加粒重来达到增产的目的。
大豆产量由株型、结荚率、荚粒数、种子百粒重等因素组成,其中粒重是遗传力最高的因素。粒重对产量的影响不限于豆科植物,对其它单双子叶植物也是产量潜力的重要因素,因此成为作物品种选育过程中要考虑的重要选择性状。已有的研究表明,粒重受栽培环境和遗传的影响。在正常的栽培条件下,遗传,也即相关基因起重要作用。因此与粒重相关的分子机制的研究成为热点。
JAZ为茉莉酸信号途径的重要抑制子,在植物生长、发育、抗生物胁迫和非生物胁迫中起作用。番茄中,SIJAZ2的过表达促进了开花和侧芽生长,拟南芥中,JAZ3与花青素的积累和病原菌的防御相关。迄今为止尚未见JAZ家族与种子粒重相关的报道。
发明内容
本发明所要解决的技术问题是如何调控植物粒重。
为了解决以上技术问题,本发明提供了一种蛋白质,命名为GmJAZ3,来源于大豆(Glycine max(L.)Merrill),为如下A1)或A2)或A3)任一所示蛋白质:
A1)由序列表中序列2所示的氨基酸序列组成的蛋白质;
A2)序列表中序列2所示的蛋白质的N端或/和C端连接蛋白标签得到的融合蛋白;
A3)将序列表中序列2所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加得到的与A1)所示的蛋白质具有90%以上的同一性且功能相同的蛋白质。
上述蛋白质中,序列表中的序列2由319个氨基酸残基组成。
上述蛋白质可人工合成,也可先合成其编码基因,再进行生物表达得到。
上述蛋白质中,蛋白标签(protein-tag)是指利用DNA体外重组技术,与目的蛋白一起融合表达的一种多肽或者蛋白,以便于目的蛋白的表达、检测、示踪和/或纯化。所述蛋白标签可为Flag标签、His标签、MBP标签、HA标签、myc标签、GST标签和/或SUMO标签等。
上述蛋白质中,同一性是指氨基酸序列的同一性。可使用国际互联网上的同源性检索站点测定氨基酸序列的同一性,如NCBI主页网站的BLAST网页。例如,可在高级BLAST2.1中,通过使用blastp作为程序,将Expect值设置为10,将所有Filter设置为OFF,使用BLOSUM62作为Matrix,将Gap existence cost,Per residue gap cost和Lambda ratio分别设置为11,1和0.85(缺省值)并进行检索一对氨基酸序列的同一性进行计算,然后即可获得同一性的值(%)。
上述蛋白质中,所述90%以上的同一性可为至少91%、92%、95%、96%、98%、99%或100%的同一性。
本发明提供了GmJAZ3在调控植物粒重中的应用。
与GmJAZ3相关的生物材料也属于本发明的保护范围,本发明还提供了与GmJAZ3相关的生物材料的新用途。
本发明提供了与GmJAZ3相关的生物材料在调控植物粒重中的应用。
本发明与GmJAZ3相关的生物材料为下述B1)-B9)中的任一种:
B1)编码GmJAZ3的核酸分子;
B2)含有B1)所述核酸分子的表达盒;
B3)含有B1)所述核酸分子的重组载体、或含有B2)所述表达盒的重组载体;
B4)含有B1)所述核酸分子的重组微生物、或含有B2)所述表达盒的重组微生物、或含有B3)所述重组载体的重组微生物;
B5)含有B1)所述核酸分子的转基因植物细胞系、或含有B2)所述表达盒的转基因植物细胞系;
B6)含有B1)所述核酸分子的转基因植物组织、或含有B2)所述表达盒的转基因植物组织;
B7)含有B1)所述核酸分子的转基因植物器官、或含有B2)所述表达盒的转基因植物器官;
B8)降低GmJAZ3表达的核酸分子;
B9)含有B8)所述核酸分子的表达盒、重组载体、重组微生物或转基因植物细胞系。
其中,所述核酸分子可以是DNA,如cDNA、基因组DNA或重组DNA;所述核酸分子也可以是RNA,如mRNA或hnRNA等。
上述生物材料中,B1)所述核酸分子具体可为如下C1)或C2)中任一所示:
C1)编码序列为序列表中序列1所示的cDNA分子或DNA分子;
C2)在严格条件下与C1)或C2)限定的DNA分子杂交,且编码GmJAZ3的DNA分子。
其中,序列表中的序列1由960个核苷酸组成,编码序列表中的序列2所示的蛋白质。
上述生物材料中,B2)所述的表达盒是指能够在宿主细胞中表达GmJAZ3的DNA,该DNA不但可包括启动GmJAZ3基因转录的启动子,还可包括终止GmJAZ3转录的终止子。进一步,所述表达盒还可包括增强子序列。可用于本发明的启动子包括但不限于:组成型启动子,组织、器官和发育特异的启动子,和诱导型启动子。启动子的例子包括但不限于:花椰菜花叶病毒的组成型启动子35S;来自西红柿的创伤诱导型启动子,亮氨酸氨基肽酶(″LAP″,Chao等人(1999)Plant Physiol 120:979-992);来自烟草的化学诱导型启动子,发病机理相关1(PR1)(由水杨酸和BTH(苯并噻二唑-7-硫代羟酸S-甲酯)诱导);西红柿蛋白酶抑制剂II启动子(PIN2)或LAP启动子(均可用茉莉酮酸甲酯诱导);热休克启动子(美国专利5,187,267);四环素诱导型启动子(美国专利5,057,422);种子特异性启动子,如谷子种子特异性启动子pF128(CN101063139B(中国专利2007 1 0099169.7)),种子贮存蛋白质特异的启动子(例如,菜豆球蛋白、napin,oleosin和大豆beta conglycin的启动子(Beachy等人(1985)EMBO J.4:3047-3053)。它们可单独使用或与其它的植物启动子结合使用。此处引用的所有参考文献均全文引用。合适的转录终止子包括但不限于:农杆菌胭脂碱合成酶终止子(NOS终止子)、花椰菜花叶病毒CaMV 35S终止子、tml终止子、豌豆rbcS E9终止子和胭脂氨酸和章鱼氨酸合酶终止子(参见,例如:Odell等人(I985)Nature 313:810;Rosenberg等人(1987)Gene,56:125;Guerineau等人(1991)Mol.Gen.Genet,262:141;Proudfoot(1991)Cell,64:671;Sanfacon等人Genes Dev.,5:141;Mogen等人(1990)Plant Cell,2:1261;Munroe等人(1990)Gene,91:151;Ballad等人(1989)Nucleic Acids Res.17:7891;Joshi等人(1987)Nucleic Acid Res.,15:9627)。
上述生物材料中,可用植物表达载体构建含有所述GmJAZ3编码基因GmJAZ3表达盒的重组表达载体。所述植物表达载体可为Gateway系统载体或双元农杆菌载体等,如pGWB411、pGWB412、pGWB405、pBin438、pCAMBIA1302、pCAMBIA2301、pCAMBIA1301、pCAMBIA1300、pBI121、pCAMBIA1391-Xa或pCAMBIA1391-Xb。使用GmJAZ3构建重组表达载体时,在其转录起始核苷酸前可加上任何一种增强型、组成型、组织特异型或诱导型启动子,如花椰菜花叶病毒(CAMV)35S启动子、泛生素基因Ubiqutin启动子(pUbi)等,它们可单独使用或与其它的植物启动子结合使用;此外,使用本发明的基因构建植物表达载体时,还可使用增强子,包括翻译增强子或转录增强子,这些增强子区域可以是ATG起始密码子或邻接区域起始密码子等,但必需与编码序列的阅读框相同,以保证整个序列的正确翻译。所述翻译控制信号和起始密码子的来源是广泛的,可以是天然的,也可以是合成的。翻译起始区域可以来自转录起始区域或结构基因。
为了便于对转基因植物细胞或植物进行鉴定及筛选,可对所用植物表达载体进行加工,如加入可在植物中表达的编码可产生颜色变化的酶或发光化合物的基因(GUS基因、萤光素酶基因等)、具有抗性的抗生素标记物(庆大霉素标记物、卡那霉素标记物等)或是抗化学试剂标记基因(如抗除莠剂基因)等。
上述生物材料中,所述重组微生物具体可为酵母,细菌,藻和真菌。
上述应用中,所述调控可为提高。
本发明进一步提供了上述GmJAZ3或上述GmJAZ3相关的生物材料在培育高粒重的转基因植物中的应用;
或,上述GmJAZ3或上述GmJAZ3相关的生物材料在植物育种中的应用。
上述应用中,植物育种中的应用具体可为将含有所述GmJAZ3或所述生物材料(如GmJAZ3编码基因GmJAZ3)的植物与其它植物进行杂交以进行植物育种。
本发明还提供一种培育高粒重的转基因植物的方法。
本发明所提供的培育高粒重的转基因植物的方法,包括提高受体植物中所述GmJAZ3的含量和/或活性,得到转基因植物;所述转基因植物的粒重高于受体植物的粒重。
上述提高受体植物中上述GmJAZ3的表达量和/或活性的方法为在受体植物中过表达GmJAZ3。
上述过表达的方法为将GmJAZ3的编码基因导入受体植物。
上述将GmJAZ3的编码基因导入受体植物可通过携带有本发明基因GmJAZ3的植物表达载体导入受体种子植物中。携带有本发明基因GmJAZ3的植物表达载体可通过使用Ti质粒、Ri质粒、植物病毒载体、直接DNA转化、微注射、电导、农杆菌介导等常规生物学方法转化植物细胞或组织,并将转化的植物细胞或组织培育成植株。
携带有本发明基因GmJAZ3的植物表达载体可为pGWB411-GmJAZ3。pGWB411-GmJAZ3是将序列表中序列1所示的DNA分子重组到载体pGWB411中得到的GmJAZ3基因表达载体。
上述GmJAZ3的编码基因的核苷酸序列是序列表中序列1所示的DNA分子。
上述应用或方法中,所述植物为单子叶植物或双子叶植物。所述双子叶植物可为十字花科植物。
本发明将GmJAZ3转入野生型拟南芥中得到的转基因拟南芥种子的粒重显著高于野生型拟南芥(野生型拟南芥对照、GmJAZ3过表达株系OE1、OE2、OE3种子千粒重分别为16.3±1.1,27.5±1.9,29.4±0.6,29.1±0.5毫克),说明GmJAZ3及其编码基因可以调控植物种子的粒重,过表达后提高了植物种子粒重。GmJAZ3及其相关生物材料可用于提高作物产量,培育高产品种。
附图说明
图1为GmJAZ3在大豆各器官中的表达特性图。
图3为GmJAZ3过表达拟南芥纯系和野生型拟南芥对照中GmJAZ3的相对表达量。
图4为GmJAZ3过表达拟南芥纯系和野生型拟南芥对照种子千粒重比较,图中**表示与野生型拟南芥对照相比,具有极显著差异。
具体实施方式
以下的实施例便于更好地理解本发明,但并不限定本发明。下述实施例中所使用的实验方法如无特殊说明,均为常规方法。下述实施例中所用的材料、试剂等,如无特殊说明,均可从商业途径得到。
下述实施例中的HN44为大豆黑农44(该品种记载于:满为群等,大豆新品种黑农44的选育及不同种植方式对其产量和品种的影响,黑龙江农业科学2004年5期,1-5;由黑龙江农业科学院大豆研究所2002年经黑龙江省农作物品种审定委员会审定的大豆品种,第一育成人为杜维广研究员,专利号为:CNA20020216.2,审定号为:黑审豆2002003)。
下述实施例中的表达载体pGWB411(该载体记载于:Tsuyoshi Nakagawa,et al.,Gatway Vectors for Plant Transformation,Plant Biotechnology,2009,26,275-284)由Tsuyoshi Nakagawa博士提供,公众经Tsuyoshi Nakagawa博士同意后可从中国科学院遗传与发育生物学研究所获得,该生物材料只为重复本发明的相关实验所用,不可作为其它用途使用。
下述实施例中的农杆菌GV3101(该农杆菌记载于:Lee CW,et al.,Agrobacteriumtumefaciens promotes tumor induction by modulating pathogen defense inArabidopsis thaliana,Plant Cell,2009,21(9),2948-62),公众可从中国科学院遗传与发育生物学研究所获得,该生物材料只为重复本发明的相关实验所用,不可作为其它用途使用。
实施例1与种子粒重相关蛋白质GmJAZ3的编码基因GmJAZ3的cDNA克隆
根据大豆种子发育过程转录组构建了调控网络,发现基因GmJAZ3为一个调控网络的节点基因,对该基因的功能进行了检测,如图1所示,发现GmJAZ3在大豆花中的转录量最高,其次是叶,在苗、荚和种子中转录量较低,根中转录量最低。
提取HN44幼苗的总RNA,将RNA用逆转录酶反转录合成cDNA。
根据在PlantGDB的大豆基因组序列(Glyma.09G123600)及测定的HN44基因组序列中GmJAZ3全长cDNA序列的信息,设计引物,引物序列如下:
GmJAZ3-up:5’-ATGGAGAGAGATTTTCTGGGTCT
GmJAZ3-dp:5’-TTATCTGAAGTAATCTCCTGCGCTT
以HN44的cDNA为模板,用GmJAZ3-up和GmJAZ3-dp为引物,进行PCR扩增,得到约1.0Kb的PCR产物,即为基因GmJAZ3,经过测序,该PCR产物的大小为960bp,具有序列表中序列1所示的核苷酸序列,将该基因编码的蛋白命名为GmJAZ3,蛋白GmJAZ3的氨基酸序列如序列表中的序列2所示。
实施例2GmJAZ3过表达拟南芥的获得
一、获得重组载体pGWB411-GmJAZ3
基因克隆使用invitrogen公司提供的Gateway系统,载体3′-T突出端,用于直接连接Taq酶扩增的PCR产物。运用TA克隆的原理将实施例1得到的基因GmJAZ3连接在克隆载体上(图2中A所示),得到重组载体pTOPO-GmJAZ3。pTOPO-GmJAZ3和表达载体pGWB411上均带有重组位点attL1和attL2,含有GmJAZ3的pTOPO-GmJAZ3与表达载体pGWB411在重组酶的作用下进行LR重组反应,最终将目的基因GmJAZ3成功构建到表达载体pGWB411上,将得到的重组载体命名为pGWB411-GmJAZ3(图2中B所示)。pGWB411-GmJAZ3是将序列表中序列1所示的DNA分子重组到表达载体pGWB411中得到的GmJAZ3基因表达载体。
二、重组农杆菌的获得
将步骤一得到的含有GmJAZ3的重组载体pGWB411-GmJAZ3用电击法导入农杆菌GV3101得到含有pGWB411-GmJAZ3的重组农杆菌,将其命名为重组农杆菌GV3101/GmJAZ3。
二、转GmJAZ3拟南芥的获得及鉴定
将重组农杆菌GV3101/GmJAZ3培养至对数期,然后用抽真空法将其转化哥伦比亚生态型拟南芥(Col-0)(种子来自Arabidopsis Biological Resource Center(ABRC))中,经培育后收获种子(T1代),将种子播于含卡那霉素(50mg/L)的MS筛选培养基上,待筛选得到的T1代植株长至4-6叶时移到蛭石上生长,收获T1代单株,各单株种子(T2代)分别播种,用相同的MS筛选培养基继续筛选以观察T2代的分离情况,如此重复数代直至获得遗传稳定的转基因纯合株系,获得15个转GmJAZ3拟南芥纯系(T5代)。随机取3个株系(命名为OE1、OE2和OE3)进行GmJAZ3基因表达量的检测,分别提取上述这3个株系和哥伦比亚生态型拟南芥(Col-0,作为野生型拟南芥对照,简称对照)的幼苗总RNA,进行反转录,分别以反转录得到的cDNA作为模板,引物为:GmJAZ3-up:5’-ATGGAGAGAGATTTTCTGGGTCT-3’和GmJAZ3-dp:5’-TTATCTGAAGTAATCTCCTGCGCTT-3’,进行Real Time-PCR鉴定。拟南芥AtActin2基因为内标,所用引物为Primer-TF:5’-ATGCCCAGAAGTCTTGTTCC-3’和Primer-TR:5’-TGCTCATACGGTCAGCGATA-3’。以内标AtActin2基因的表达量为1,测定GmJAZ3基因的相对表达量。实验重复三次,结果取平均值,结果如图3所示,OE1、OE2和OE3中GmJAZ3的相对表达量分别约为0.016、0.021和0.041,在对照中未能检测出GmJAZ3的表达量。
上述结果进一步证明,GmJAZ3转入拟南芥中,且得到表达。OE1、OE2和OE3这3个株系为GmJAZ3过表达株系。
三、转GmJAZ3基因拟南芥的表型分析
检测野生型拟南芥对照、GmJAZ3过表达株系OE1、OE2和OE3在正常条件下的表型。结果表明,野生型拟南芥对照和GmJAZ3过表达株系OE1、OE2和OE3的表型如莲座、株高等均与对照无显著差异。
测量野生型拟南芥对照、GmJAZ3过表达株系OE1、OE2和OE3的种子千粒重,即干燥的种子千粒重。每个株系取20株的种子,每个株系称量200粒种子,生物学实验重复三次,结果取平均值±标准差。
结果如图4所示,野生型拟南芥对照、GmJAZ3过表达株系OE1、OE2、OE3的种子千粒重分别为16.1±0.8,19.5±1.0,21.8±2.1和19.9±0.9毫克。统计表明,3个GmJAZ3过表达拟南芥纯系种子千粒重均极显著高于野生型拟南芥对照。
以上表明,GmJAZ3的过量表达提高了转基因植株种子千粒重,并且其在提高转基因植株种子重量(粒重)的同时,没有明显影响植株的正常生长。因此,GmJAZ3基因可作为提高植物种子产量的目的基因,GmJAZ3是与种子重量相关的蛋白质。
以上对本发明进行了详述。对于本领域技术人员来说,在不脱离本发明的宗旨和范围,以及无需进行不必要的实验情况下,可在等同参数、浓度和条件下,在较宽范围内实施本发明。虽然本发明给出了特殊的实施例,应该理解为,可以对本发明作进一步的改进。总之,按本发明的原理,本申请欲包括任何变更、用途或对本发明的改进,包括脱离了本申请中已公开范围,而用本领域已知的常规技术进行的改变。按以下附带的权利要求的范围,可以进行一些基本特征的应用。
序列表
<110> 中国科学院遗传与发育生物学研究所
<120> 与植物粒重相关蛋白GmJAZ3及其编码基因和应用
<130> GNCFY191301
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 960
<212> DNA
<213> 大豆属大豆(Glycine max L. Merrill)
<400> 1
atggagagag attttctggg tctgagctca aaagaaccat tggctatgat gaaggaagag 60
atgaatattg atattggaag caaagacaca ggtttcacaa atggagcagt ggtaaaatgg 120
cccttcatga acaaagtata tgtccatcct cttttgatgt cattcaatcc tcctcaggtg 180
aattctgtga accatcctca tggtatgaag atgttttcag ttcccaatca agcaatttca 240
gtttccatgg gacatccatc cctaaagaat cattttgcaa ctgttggtca gaatatgaat 300
gttgctactg tgaagcaacc actactcgga ggaatacctg ttacggtgcc tcattcagtt 360
catcccattg ttggtgctgt tgctggaatg accgaaacac gcgtgaaacc atccgtacct 420
gctcctaaac ttacaatctt ctatgcgggc actgtgaatg tctttgaaga tatctcccct 480
gagaaggcac aagctatcat gttgttggct gggaatggct tatcagctgg ttctaatgag 540
gcatcaccca atgttcaggc gccctgcttg aagttggcag taggtgatgg tgtgcctgtt 600
agtcaaccca taccaccctg ctccggtctt tcaagtcctt catctgtttc ttcacatact 660
ggttcaccgt cagggagtgg ttcaagtagc aatgatgagt ttctcgcagc taaaacatct 720
ggaggtacca cttcctctgt taacaaagtg gagactccga aagtagtcaa cacaactacc 780
atgctaacat cagctgtgcc acaggctcgc aaggcatcat tagctcgatt tttggagaag 840
cgcaaggaaa gggtgatgaa tgcagcacca tataacctca acaagaagtc tgagaatgtg 900
ccacagcaga atacaatggt ttctaacatc actgcaagcg caggagatta cttcagataa 960
<210> 2
<211> 319
<212> PRT
<213> 大豆属大豆(Glycine max L. Merrill)
<400> 2
Met Glu Arg Asp Phe Leu Gly Leu Ser Ser Lys Glu Pro Leu Ala Met
1 5 10 15
Met Lys Glu Glu Met Asn Ile Asp Ile Gly Ser Lys Asp Thr Gly Phe
20 25 30
Thr Asn Gly Ala Val Val Lys Trp Pro Phe Met Asn Lys Val Tyr Val
35 40 45
His Pro Leu Leu Met Ser Phe Asn Pro Pro Gln Val Asn Ser Val Asn
50 55 60
His Pro His Gly Met Lys Met Phe Ser Val Pro Asn Gln Ala Ile Ser
65 70 75 80
Val Ser Met Gly His Pro Ser Leu Lys Asn His Phe Ala Thr Val Gly
85 90 95
Gln Asn Met Asn Val Ala Thr Val Lys Gln Pro Leu Leu Gly Gly Ile
100 105 110
Pro Val Thr Val Pro His Ser Val His Pro Ile Val Gly Ala Val Ala
115 120 125
Gly Met Thr Glu Thr Arg Val Lys Pro Ser Val Pro Ala Pro Lys Leu
130 135 140
Thr Ile Phe Tyr Ala Gly Thr Val Asn Val Phe Glu Asp Ile Ser Pro
145 150 155 160
Glu Lys Ala Gln Ala Ile Met Leu Leu Ala Gly Asn Gly Leu Ser Ala
165 170 175
Gly Ser Asn Glu Ala Ser Pro Asn Val Gln Ala Pro Cys Leu Lys Leu
180 185 190
Ala Val Gly Asp Gly Val Pro Val Ser Gln Pro Ile Pro Pro Cys Ser
195 200 205
Gly Leu Ser Ser Pro Ser Ser Val Ser Ser His Thr Gly Ser Pro Ser
210 215 220
Gly Ser Gly Ser Ser Ser Asn Asp Glu Phe Leu Ala Ala Lys Thr Ser
225 230 235 240
Gly Gly Thr Thr Ser Ser Val Asn Lys Val Glu Thr Pro Lys Val Val
245 250 255
Asn Thr Thr Thr Met Leu Thr Ser Ala Val Pro Gln Ala Arg Lys Ala
260 265 270
Ser Leu Ala Arg Phe Leu Glu Lys Arg Lys Glu Arg Val Met Asn Ala
275 280 285
Ala Pro Tyr Asn Leu Asn Lys Lys Ser Glu Asn Val Pro Gln Gln Asn
290 295 300
Thr Met Val Ser Asn Ile Thr Ala Ser Ala Gly Asp Tyr Phe Arg
305 310 315
Claims (8)
1.如下A1)或A2)或A3)任一所示蛋白质在提高植物粒重中的应用:
A1)由序列表中序列2所示的氨基酸序列组成的蛋白质;
A2)序列表中序列2所示的蛋白质的N端或/和C端连接蛋白标签得到的融合蛋白;
A3)来源于大豆与A1)所示的蛋白质具有99%以上的同一性且功能相同的蛋白质;
所述植物为拟南芥和大豆。
2.与权利要求1中所述的蛋白质相关的生物材料在提高植物粒重中的应用;
所述生物材料为下述B1)-B7)中的任一种:
B1)编码权利要求1所述蛋白质的核酸分子;
B2)含有B1)所述核酸分子的表达盒;
B3)含有B1)所述核酸分子的重组载体、或含有B2)所述表达盒的重组载体;
B4)含有B1)所述核酸分子的重组微生物、或含有B2)所述表达盒的重组微生物、或含有B3)所述重组载体的重组微生物;
B5)含有B1)所述核酸分子的转基因植物细胞系、或含有B2)所述表达盒的转基因植物细胞系;
B6)含有B1)所述核酸分子的转基因植物组织、或含有B2)所述表达盒的转基因植物组织;
B7)含有B1)所述核酸分子的转基因植物器官、或含有B2)所述表达盒的转基因植物器官;
所述植物为拟南芥和大豆。
3.根据权利要求2所述的应用,其特征在于:B1)所述核酸分子为序列表中序列1所示的cDNA分子或DNA分子。
4.权利要求1中所述的蛋白质或权利要求2或3中所述的生物材料在培育高粒重的转基因植物中的应用;所述植物为拟南芥和大豆。
5.一种培育高粒重的转基因植物的方法,其特征在于:提高受体植物中权利要求1中所述的蛋白质的含量,得到转基因植物;所述转基因植物的粒重高于受体植物的粒重;
所述植物为拟南芥和大豆。
6.根据权利要求5所述的方法,其特征在于:所述提高受体植物中权利要求1中所述蛋白质的含量的方法为在受体植物中过表达权利要求1中所述蛋白质。
7.根据权利要求6所述的方法,其特征在于:所述过表达的方法为将权利要求1中所述蛋白质的编码基因导入受体植物中。
8.根据权利要求5-7中任一项所述的方法,其特征在于:所述蛋白质的编码基因的核苷酸序列如序列表中序列1所示。
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012117368A1 (en) * | 2011-03-01 | 2012-09-07 | Basf Plant Science Company Gmbh | Plants having enhanced yield-related traits and producing methods thereof |
CN103570813A (zh) * | 2012-07-26 | 2014-02-12 | 中国农业科学院棉花研究所 | 与植物抗逆性相关蛋白Gh01399及其编码基因与应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090265815A1 (en) * | 2000-08-09 | 2009-10-22 | Nickolai Alexandrov | Sequence-determined DNA fragments and corresponding polypeptides encoded therapy |
US20130326723A1 (en) * | 1999-05-06 | 2013-12-05 | Thomas J. La Rosa | Soy nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
US20040031072A1 (en) * | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
-
2019
- 2019-06-25 CN CN201910558178.0A patent/CN112125964B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012117368A1 (en) * | 2011-03-01 | 2012-09-07 | Basf Plant Science Company Gmbh | Plants having enhanced yield-related traits and producing methods thereof |
CN103582702A (zh) * | 2011-03-01 | 2014-02-12 | 巴斯夫植物科学有限公司 | 具有增强的产量相关性状的植物和用于制备该植物的方法 |
CN103570813A (zh) * | 2012-07-26 | 2014-02-12 | 中国农业科学院棉花研究所 | 与植物抗逆性相关蛋白Gh01399及其编码基因与应用 |
Non-Patent Citations (8)
Title |
---|
A PP2C-1 Allele Underlying a Quantitative Trait Locus Enhances Soybean 100-Seed Weight;Xiang Lu 等;《Mol Plant》;20170328;第10卷(第5期);第670-684页 * |
Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing;Xinpeng Qi 等;《Nat Commun》;20140709(第5期);第4340页 * |
PREDICTED: Glycine max protein TIFY 6B (LOC100799314), mRNA;GenBank;《GenBank》;20180831;XM_003533907.3 * |
protein TIFY 6B [Glycine max];GenBank;《GenBank》;20180831;XP_003533955.1 * |
Protein TIFY 6B [Glycine soja];GenBank;《GenBank》;20141217;KHN41401.1 * |
The transcriptomic signature of developing soybean seeds reveals the genetic basis of seed trait adaptation during domestication;Xiang Lu 等;《Plant J》;20160620;第86卷(第6期);第530-544页 * |
UniProtKB-I1L2W4 (I1L2W4_SOYBN);UniProtKB;《UniProtKB》;20120613;I1L2W4 * |
基于Meta分析的大豆百粒重的QTLs定位;齐照明 等;《中国农业科学》;20091130;第42卷(第11期);第3795-3803页 * |
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