CN112106900B - Feed for improving egg laying performance and oviduct development of laying hens and preparation method thereof - Google Patents

Feed for improving egg laying performance and oviduct development of laying hens and preparation method thereof Download PDF

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Publication number
CN112106900B
CN112106900B CN202010898690.2A CN202010898690A CN112106900B CN 112106900 B CN112106900 B CN 112106900B CN 202010898690 A CN202010898690 A CN 202010898690A CN 112106900 B CN112106900 B CN 112106900B
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feed
parts
laying
oviduct
egg
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CN112106900A (en
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段海涛
贠婷婷
于翠萍
崔耀明
黄安群
任文义
席磊
程璞
刘卫东
石志芳
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Henan Jumao Biotechnology Co ltd
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Henan University of Animal Husbandry and Economy
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284Atractylodes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/286Carthamus (distaff thistle)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484Glycyrrhiza (licorice)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/74Rubiaceae (Madder family)
    • A61K36/748Oldenlandia or Hedyotis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/899Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8994Coix (Job's tears)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention belongs to the technical field of feeds, and particularly relates to a feed for improving egg laying performance and oviduct development of laying hens and a preparation method thereof. A feed for improving egg laying performance and oviduct development of laying hens, wherein lactic acid bacteria and saccharomycetes are added into basic raw materials for mixed fermentation, and the fermented materials are mixed with hop crushed materials; the basic raw materials comprise, by weight, 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed. The feed for improving egg laying performance and oviduct development of the laying hen provided by the invention has the advantages that the strain effectively releases the active ingredients in the natural plant feed raw materials, the active ingredients recover oviduct damage, the integrity of epithelial cells is maintained, the occurrence of oviduct inflammation is reduced, the glossiness of eggshells is good, the egg laying of the laying hen is promoted, the egg weight is increased, the color of egg yolk is improved, and the egg quality is improved.

Description

Feed for improving egg laying performance and oviduct development of laying hens and preparation method thereof
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a feed for improving egg laying performance and oviduct development of laying hens and a preparation method thereof.
Background
Salpingitis is liable to occur when there are many bacteria, virulence or body resistance is reduced. Salpingitis is a common disease in fact on laying hens, namely, the oviduct has inflammation, and yellow and white pus-like secretion, even yellow and white cheese secretion, is often discharged in the oviduct, so that the perianal and feather are polluted. Mainly manifested by pain and uneasiness of chickens, the eggs which are laid often have bloodstains, and the eggs which are malformed seriously even do not lay. The existing mode for treating the salpingitis of the chickens is mostly western medicine antibiotic treatment.
At present, along with the coming of the times of 'resistance reduction' and 'no resistance', an antibiotic substitute product is searched, and natural, safe and resistance-reduced animal products are produced, so that the method has important practical significance for sustainable development of animal husbandry. At present, the oviduct inflammation is treated by antibiotics, which not only damages the health of animals, but also has great harm to the environment. In the social setting of tightening food safety, limitation of antibiotic residue, pursuing dietary health, the use of antibiotics to treat salpingitis has not met the development of the era.
Disclosure of Invention
In order to solve the problems, the invention provides a feed for improving egg laying performance and oviduct development of laying hens and a preparation method thereof.
The object of the invention is achieved in the following way: a feed for improving egg laying performance and oviduct development of laying hens, wherein lactic acid bacteria and saccharomycetes are added into basic raw materials for mixed fermentation, and the fermented materials are mixed with hop crushed materials; the basic raw materials comprise, by weight, 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed.
The preparation method of the feed for improving egg laying performance and oviduct development of the laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed;
(2) Mixing lactobacillus and yeast fermentation broth or dissolving lactobacillus and yeast powder in water/nutrient solution for activation;
(3) Mixing the solution obtained in the step (2) with a basic raw material, and then fermenting;
(4) Mixing the fermented material with the hop powder to obtain the feed.
In the step (1), the rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed are crushed, sieved and fermented.
Pulverizing rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed to 400-500 mu m, and then fermenting.
The fermentation conditions are as follows: the temperature is 18-45 ℃, the moisture content is 20-50%, and the initial pH value is 5.5-8.0.
The total number of viable bacteria in the fermented material reaches more than 105CFU/g, and the pH value reaches between 3.0 and 6.8.
And (3) spraying the solution obtained in the step (2) onto the basic raw materials.
Inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
The fermented feed is mixed with the hop powder in a mixing ratio of 6:1-8:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product is 0.5-1%.
The beer pollen crushed material is obtained by crushing hops to 60-100 meshes.
The feed for improving the egg laying performance and the oviduct development of the laying hen can effectively recover oviduct injury, maintain the integrity of epithelial cells, reduce the occurrence of oviduct inflammation, ensure that the eggshell has good glossiness, promote the laying of the laying hen, and improve the egg weight and single quality.
The feed for improving egg laying performance and oviduct development of the laying hen provided by the invention has the advantages that the strain effectively releases the active ingredients in the natural plant feed raw materials, the active ingredients recover oviduct damage, the integrity of epithelial cells is maintained, the occurrence of oviduct inflammation is reduced, the glossiness of eggshells is good, the egg laying of the laying hen is promoted, the egg weight is increased, the color of egg yolk is improved, and the egg quality is improved.
According to the feed for improving egg laying performance and oviduct development of the laying hen, hop components are added into fermented materials, alpha-acid and beta-acid of the hop components can synergistically enhance the functions of basic raw material components, improve the health condition of an organism, enable effective components to recover oviduct damage, maintain the integrity of epithelial cells, reduce the occurrence of oviduct inflammation, enable the glossiness of egg shells to be good, promote egg laying of the laying hen, improve egg weight and single quality, and have important significance for development of animal husbandry.
The feed for improving egg laying performance and oviduct development of the laying hen provided by the invention has the advantages that the natural plant feed raw materials have no drug residues and no environmental pollution, and the problem of drug residues caused by abuse of antibiotics can be completely solved.
Detailed Description
A feed for improving egg laying performance and oviduct development of laying hens, wherein lactic acid bacteria and saccharomycetes are added into basic raw materials for mixed fermentation, and the fermented materials are mixed with hop crushed materials; the basic raw materials comprise, by weight, 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed.
The preparation method of the feed for improving egg laying performance and oviduct development of the laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed;
(2) Mixing lactobacillus and yeast fermentation broth or dissolving lactobacillus and yeast powder in water/nutrient solution for activation;
(3) Mixing the solution obtained in the step (2) with a basic raw material, and then fermenting;
(4) Mixing the fermented material with the hop powder to obtain the feed.
In the step (1), the rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed are crushed, sieved and fermented.
Pulverizing rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed to 400-500 mu m, and then fermenting.
The fermentation conditions are as follows: the temperature is 18-45 ℃, the moisture content is 20-50%, and the initial pH value is 5.5-8.0.
The total number of viable bacteria in the fermented material reaches more than 105CFU/g, and the pH value reaches between 3.0 and 6.8.
And (3) spraying the solution obtained in the step (2) onto the basic raw materials.
Inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
The fermented feed is mixed with the hop powder in a mixing ratio of 6:1-8:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product is 0.5-1%.
The beer pollen crushed material is obtained by crushing hops to 60-100 meshes.
Rhizoma atractylodis: xin Wenwei they are bitter in property and enter spleen, stomach and liver meridians. Has the functions of invigorating spleen, eliminating dampness, dispelling wind and dispelling cold. Clinically, the traditional Chinese medicine composition is used for treating dampness obstruction of spleen and stomach, inappetence, diarrhea, edema, beriberi, phlegm sac, rheumatism, arthralgia, common cold due to wind-cold, sparrow eye night blindness and the like. Modern pharmacological researches prove that rhizoma atractylodis has unique chemical components and remarkable biological activity, and is a relatively deep person in the aspects of resisting arrhythmia, protecting liver, promoting urination and the like.
Herba Hedyotidis Diffusae: bitter and sweet in taste, cold in nature; stomach, large intestine and small intestine channels; has the main effects of clearing away heat and toxic materials, relieving pain, resolving hard mass, promoting urination and removing dampness. The main indications are: 1. carbuncle, sore throat, snake bite: it is bitter and cold, has strong actions of clearing heat and removing toxicity, and can be used for all kinds of symptoms caused by heat toxin, both orally and externally. 2. Heat stranguria with pain: it is sweet and cold in nature and has the actions of clearing heat, promoting diuresis and treating stranguria. 3. It can clear heat and promote diuresis, and can be used for damp-heat jaundice.
Licorice root: sweet in nature and flat in flavor. It enters meridians and enters heart, lung, spleen and stomach meridians. The main functions are as follows: spleen invigorating, qi replenishing, heat and toxic materials clearing away, phlegm eliminating, cough relieving, pain relieving, and medicines regulating. Can be used for treating weakness of spleen and stomach, listlessness, debilitation, palpitation, short breath, cough with excessive phlegm, abdominal pain, limb spasm, carbuncle, swelling, sore and toxic materials, and relieving drug toxicity and intensity.
Safflower: pungent and warm in nature. Enter meridians and enter heart and liver meridians. Has the functions of promoting blood circulation, dredging channels, removing blood stasis and relieving pain. Can be used for treating amenorrhea, dysmenorrhea, lochia, abdominal mass, chest pain, abdominal pain due to stagnation, chest and hypochondrium stabbing pain, traumatic injury, and pyocutaneous disease swelling and pain. Has effects of promoting blood circulation, removing blood stasis, eliminating dampness, and removing swelling.
Semen Coicis: sweet and bland in flavor and cool. It enters spleen, stomach and lung meridians. Has effects of promoting water permeation, invigorating spleen, relieving diarrhea, removing arthralgia, expelling pus, removing toxic substances, and resolving hard mass. Is mainly used for treating symptoms such as edema, beriberi, difficult urination, spleen deficiency diarrhea, cancer and the like.
Hops: also called as famous hop, negligence cloth, wild hop, hops, yeast flower, tangcao flower, snake twist, etc., is a twined herb plant of hermaphrodite plants. Hops are one of the essential important raw materials in the beer industry, and are indispensable and irreplaceable in beer brewing. Bitter and slightly cool. Stomach invigorating, digestion promoting, tranquilizing, and urination promoting. Can be used for treating dyspepsia, abdominal distention, edema, urination, stranguria, tuberculosis, and insomnia.
The hop has higher development and application value, (1) antibacterial: the lupulone and humulone compounds in the hop have good antibacterial effect, and especially have obvious antibacterial effect on G+ and tubercle bacillus; (2) antioxidation: research shows that cancer, aging or other diseases are mostly related to the generation of excessive free radicals, and the main antioxidant substances in hops comprise hops polyphenols, flavonoids, alpha-acid, beta-acid and the like, so that the hops can effectively remove oxygen free radicals in a body; (3) improving the immunity of the organism: the hop beta acid can directly influence intestinal microorganisms, further influence the anti-inflammatory function of host intestinal cells and immune system, and the hop extract has good inhibition effect on acute gastric mucosal injury caused by absolute ethyl alcohol of rats, chronic gastric mucosal injury caused by glacial acetic acid and gastric mucosal injury of rats caused by indomethacin, and has auxiliary protection effect on gastric mucosal injury. (4) Improving the digestion and utilization rate of nutrient substances: the side chains and oxidation products of hops beta acids have similar structures and can activate the peroxisome proliferator-activated receptor (pparα), thereby affecting lipid metabolism.
Yeast is a single-cell fungus that can survive in both aerobic and anaerobic environments and belongs to a facultative anaerobe. Active ingredients in the natural plant feed raw materials can be released in the fermentation process. Lactic acid bacteria (lactic acid bacteria, LAB) are a general name of bacteria capable of producing a large amount of lactic acid by utilizing fermentable carbohydrate, and substances such as organic acid, special enzyme system, acidophilin and the like produced by fermentation of the lactic acid bacteria have special physiological functions, so that the intestinal flora structure of the laying hen is improved, and the oviduct state is improved.
The present invention will now be described in detail with reference to specific examples, which are given herein for further illustration only and are not to be construed as limiting the scope of the invention, since numerous insubstantial modifications and adaptations thereof will now occur to those skilled in the art in light of the foregoing disclosure.
The experimental methods in the following examples are conventional methods unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified. Such as hops from Qingdao big flowers; the initial viable bacteria concentration of lactobacillus and yeast is 10 10 CFU/g, lactic acid bacteria and yeast are all from Zhengzhou cloud beneficial biotechnology Co., ltd; the initial viable bacteria concentration of the lactobacillus bacteria liquid and the saccharomycete liquid is 10 10 CFU/g, lactobacillus solution and yeast solution are all from Zhengzhou cloud beneficial biotechnology Co.
Example 1: lactic acid bacteria and saccharomycete liquid mixed fermentation
1. Pretreatment of basic raw materials: 30Kg of rhizoma atractylodis, 25Kg of oldenlandia diffusa, 25Kg of liquorice, 12Kg of safflower and 8Kg of coix seed, crushing the rhizoma atractylodis, sieving the crushed rhizoma atractylodis, the crushed coix seed into 400um particle size, and uniformly mixing the crushed rhizoma atractylodis, the herba oldenlandia diffusae, the liquorice, the safflower and the coix seed to obtain a crushed basic raw material;
fermentation strain: mixing 25Kg lactobacillus solution and 18Kg yeast solution, spraying into the crushed basic raw material, and fermenting; lactic acid bacteria liquid and saccharomycete liquidThe initial viable bacteria concentration of (2) was 10 10 CFU/g, lactobacillus liquid and saccharomycete liquid are all from Zhengzhou cloud beneficial biotechnology Co., ltd; inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
Fermentation conditions: the temperature is 30 ℃, the moisture is 20%, the initial pH value is 6.5, and fermentation is started;
after fermentation for 6 days, the content of viable bacteria in the fermentation material reaches 2.5X10 6 CFU/g, the pH value of the fermentation material is 5.5, and the special aroma and lactic acid aroma of the yeast fermentation with relatively strong smell can be smelled. Namely the fermented material.
And adding 100-mesh beer pollen crushed material according to the weight ratio, wherein the mass ratio of the fermented material to the hop crushed material is 8:1, and the total content of beta-acid and alpha-acid in the mixed product reaches 0.5%, so as to obtain the feed end product.
Example 2: activated lactobacillus and saccharomycete powder and then mixed fermentation
Pretreatment of basic raw materials: 25Kg of rhizoma atractylodis, 20Kg of oldenlandia diffusa, 20Kg of liquorice, 10Kg of safflower and 5Kg of coix seed, pulverizing into 460um particle size, sieving, and mixing to obtain pulverized basic material;
fermentation strain: dissolving 150g of lactobacillus and 150g of saccharomycete in 30Kg of water, adding 3Kg of glucose, activating for 4 hours at 35 ℃, spraying the mixture into the crushed basic raw materials, and then fermenting; the initial viable bacteria concentration of lactobacillus and yeast is 10 10 CFU/g, lactic acid bacteria and yeast are all from Zhengzhou cloud beneficial biotechnology Co., ltd; inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
Fermentation conditions: the temperature is 35 ℃, the moisture is 26.5%, the initial pH value is 6.3, and fermentation is started;
after fermentation for 6 days, the content of viable bacteria in the fermentation material reaches 3.4X10 6 CFU/g, the pH value of the fermentation material is 5.8, and the special aroma and lactic acid aroma of the yeast fermentation with relatively strong smell can be smelled. Namely the fermented material.
Example 3:
pretreatment of basic raw materials: 25Kg of rhizoma atractylodis, 20Kg of oldenlandia diffusa, 20Kg of liquorice, 10Kg of safflower and 5Kg of coix seed, pulverizing into 460um particle size, sieving, and mixing to obtain pulverized basic material;
fermentation strain: dissolving 150g of lactobacillus and 150g of saccharomycete in 30Kg of water, adding 3Kg of glucose, activating for 4 hours at 35 ℃, spraying the mixture into the crushed basic raw materials, and then fermenting; the initial viable bacteria concentration of lactobacillus and yeast is 10 10 CFU/g, lactic acid bacteria and yeast are all from Zhengzhou cloud beneficial biotechnology Co., ltd; inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
Fermentation conditions: the temperature is 35 ℃, the moisture is 26.5%, the initial pH value is 6.3, and fermentation is started;
after fermentation for 6 days, the content of viable bacteria in the fermentation material reaches 3.4X10 6 CFU/g, the pH value of the fermentation material is 5.8, and the special aroma and lactic acid aroma of the yeast fermentation with relatively strong smell can be smelled. Namely the fermented material.
And adding 80-mesh beer pollen crushed material according to the weight ratio, wherein the mass ratio of the fermented material to the hop crushed material is 6:1, and the total content of beta-acid and alpha-acid in the mixed product reaches 0.98 percent to obtain a feed end product.
Cultivation effect experiment
1. Experiment on egg laying performance of laying hen
To demonstrate the effect of the present invention, the product of example 1 was added to the diet of laying hens at 5% addition, with the blank set as a control, as follows:
the test laying hens are in an egg laying peak period, the weight of the laying hens is (0.90+/-0.06 Kg), the laying hens are divided into 4 treatments, each treatment is repeated for 6 times, 18 chickens are repeated for each treatment, the total period of feeding is 432, normal daily ration is fed to a control group, a normal daily ration formula is fed to a test group, 3 groups are respectively fed with daily ration plus additive, the additive of the test group 1 is fermented material of the embodiment 2, the additive of the test group 2 is feed of the embodiment 3, the additive of the test group is beer powder crushed material, the specific feed formulas of the test group and the control group are shown in a table 1, and no obvious difference between nutrition indexes of the test group and a blank control group is ensured; and the control group and the test group have no obvious difference in other culture conditions and drinking water. And after the test is finished, calculating the production performance and the egg quality of the laying hens. The test site is in a certain farm in Henan Hetao city.
The test formula is shown in Table 1, the egg laying performance is shown in Table 2, the egg quality is shown in Table 3, and the blood index is shown in Table 4.
Each kilogram of premix contains Provided the following per kilogram of premix:VA 318 mg and VD 3 2.25 mg,VE 860 mg,VK 112.5 mg,VB 1 45 mg,VB 2 187.5 mg,VB 6 105 mg,VB 12 0.375 mg, nicotinic acid 900 mg, pantothenic acid 30 mg, folic acid 30 mg, biotin 2.25 mg, choline 88 g,Fe 2.25 g,Cu 0.63 g,Mn2.4 g,Zn 2.38g,17 mg,Se 15 mg.
The difference between the lower case letters of the same row of data shoulder marks is obviousP<0.05 Identical or no letter indicates that the difference is not significantP>0.05). The table below is the same.
The egg laying rate and average egg weight of test group 2 were significantly higher than those of control, test group 1 and test group 3 (P < 0.05); the feed egg ratio, egg breaking rate and soft egg rate of test group 2 were significantly lower than those of the other two groups (P < 0.05).
Test group 2 egg yolk brightness was significantly higher than control, test group 1 and test group 3 (P < 0.05); the redness and yellowness of test group 3 were significantly higher than those of the remaining three groups (P < 0.05), with no significant differences between the four treatment groups of eggshell strength, eggshell thickness and egg shape index (P > 0.05).
Gu Bingsuan aminotransferase, aspartate aminotransferase, total protein and albumin were not significantly different between the four treatment groups (P > 0.05).
In conclusion, compared with the groups which only feed the fermented feed and the hop crushing groups, the fermented feed and hop mixture for the laying hens have better egg laying performance, highest egg laying rate and highest average egg weight, and the feed-egg ratio is obviously lower than that of the other groups.
2. Experiment on improving effect of oviduct inflammation of laying hen
In order to prove the improvement effect of the invention on the salpingitis of the laying hens, the laying hens suffering from salpingitis are divided into two treatment groups, one group is fed with blank ration as a control group, and the other group is fed with ration containing 5% of the product of the additive example 3 as a test group, and the specific steps are as follows:
the test laying hens are in egg laying peak period and have salpingitis, the weight of the laying hens is (0.90+/-0.06 kg), the laying hens are divided into 2 treatments, each treatment is carried out for 3 times, each treatment is carried out for 5 chickens, 30 chickens are fed for 1 week, the control group is fed with normal daily ration, and the test group is fed with: daily ration + example 3 product, the diet formulas of the specific test group and the control group are shown in table 5, so that the nutrition index of the test group is ensured to have no obvious difference from that of the blank control group; and the control group and the test group have no obvious difference in other culture conditions and drinking water. After 1 week, the egg laying performance was measured, data set table 6, inflammatory factors in blood were measured, and the results are shown in table 7.
Each kilogram of premix contains Provided the following per kilogram of premix:VA 318 mg and VD 3 2.25 mg, VE 860 mg, VK 112.5 mg,VB 1 45 mg,VB 2 187.5 mg,VB 6 105 mg, VB 12 0.375 mg, nicotinic acid 900 mg, pantothenic acid 30 mg, folic acid 30 mg, biotin 2.25 mg, choline 88 g, fe 2.25 g, cu 0.63 g, mn2.4 g, zn 2.38g,17 mg, se 15 mg.
The difference between the lower case letters of the same row of data shoulder marks is obviousP<0.05 Identical or no letter indicates that the difference is not significantP>0.05). The table below is the same.
After one week, the control group of laying hens have inappetence, messy and matt feathers, have less blood and personal shape in egg production, have more soft shell eggs and the like, have engorged and swollen cloaca of the laying hens, are polluted by yellow-white secretion, and have blood stains or yellow-white secretion on eggshells. The test group of laying hens have better recovery of oviduct inflammation symptoms, the laying rate is in an ascending trend, the inferior egg number is in a descending trend, and blood stains or yellow-white secretion are less on eggshells. The egg yield test group was significantly higher than the control group (P < 0.05), and the average egg weight test group was significantly higher than the control group (P < 0.05).
The difference between the lower case letters of the same row of data shoulder marks is obviousP<0.05 Identical or no letter indicates that the difference is not significantP>0.05). The table below is the same.
The IL-2 test group was significantly lower than the control group (P < 0.05); the IL-6 test group is obviously lower than the control group (P < 0.05), which shows that the laying hens suffering from salpingitis can relieve the salpingitis symptoms by feeding the feed of the invention.
Embodiments of the present application may also be the following embodiments. The initial viable bacteria concentration of the lactic acid bacteria liquid and the yeast liquid in the examples was 10 10 CFU/g, lactobacillus liquid and saccharomycete liquid are all from Zhengzhou cloud beneficial biotechnology Co., ltd; the initial viable bacteria concentration of lactobacillus and yeast is 10 10 CFU/g, lactic acid bacteria and yeast are all from Zhengzhou cloud beneficial biotechnology Co., ltd; inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
Example 4
A preparation method of feed for improving egg laying performance and oviduct development of laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: rhizoma Atractylodis 20Kg, herba Hedyotidis Diffusae 20Kg, glycyrrhrizae radix 20Kg, carthami flos 10Kg, coicis semen 5 Kg; pulverizing rhizoma Atractylodis, herba Hedyotidis Diffusae, glycyrrhrizae radix, carthami flos, coicis semen, and sieving to obtain 400 μm granule base material;
(2) Uniformly mixing 26Kg of lactobacillus solution and 20Kg of saccharomycete solution;
(3) Mixing the solution obtained in the step (2) with the basic raw material obtained in the step (1) to ensure that the concentration of viable bacteria reaches 10 5 Above CFU/g, then ferment, ferment condition is: the temperature is 18 ℃, the moisture content is 26.5 percent, and the initial pH value is 5.5; the total number of viable bacteria in the fermentation material reaches 2.5 multiplied by 10 6 CFU/g, pH value reaches 4.8, stop fermenting, get fermented supplies.
(4) The fermented material is mixed with the hop powder in a mixing ratio of 6:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product reaches 1.00% to obtain the feed. The beer pollen crushed material is obtained by crushing hops to 60 meshes.
Example 5
A preparation method of feed for improving egg laying performance and oviduct development of laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: rhizoma Atractylodis 30Kg, herba Hedyotidis Diffusae 30Kg, glycyrrhrizae radix 30Kg, carthami flos 15 Kg, coicis semen 10 Kg; pulverizing rhizoma Atractylodis, herba Hedyotidis Diffusae, glycyrrhrizae radix, carthami flos, coicis semen, and sieving to obtain 500 μm granule base material;
(2) 180g of lactobacillus and 160g of saccharomycete are taken and dissolved in 30Kg of water, 3.5Kg of glucose is added, and the mixture is activated for 4 hours at 35 ℃;
(3) Mixing the solution obtained in the step (2) with the basic raw material obtained in the step (1) to ensure that the concentration of viable bacteria reaches 10 5 Above CFU/g, then ferment, ferment condition is: the temperature is 45 ℃, the moisture content is 20.2 percent, and the initial pH value is 8.0; the total number of viable bacteria in the fermentation material reaches 4.2 multiplied by 10 6 And (3) stopping fermenting when the pH value reaches 6.2 above CFU/g to obtain a fermented material.
(4) The fermented material is mixed with the hop powder in a mixing ratio of 8:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product reaches between 0.55% to obtain the feed. The beer pollen crushed material is obtained by crushing hops to 100 meshes.
Example 6
A preparation method of feed for improving egg laying performance and oviduct development of laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: 22Kg of rhizoma atractylodis, 28Kg of oldenlandia diffusa, 21Kg of liquorice, 14Kg of safflower and 9Kg of coix seed; pulverizing rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed, and sieving to obtain a basic raw material with the granularity of 400-500 mu m;
(2) 180g of lactobacillus and 150g of saccharomycete are taken and dissolved in 40Kg of water, 4.5Kg of glucose is added, and the mixture is activated for 4 hours at 35 ℃;
(3) Mixing the solution obtained in the step (2) with the basic raw material obtained in the step (1) to ensure that the concentration of viable bacteria reaches 10 5 Above CFU/g, then ferment, ferment condition is: the temperature is 22 ℃, the moisture content is 28.8 percent, and the initial pH value is 6.0; the total number of viable bacteria in the fermentation material reaches 3.8x10 6 And (3) stopping fermenting when the pH value reaches 4.8 and obtaining the fermented material.
(4) The fermented material is mixed with the hop powder in a mixing ratio of 7:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product reaches between 0.76% to obtain the feed. The beer pollen crushed material is obtained by crushing hops to 80 meshes.
Example 7
A preparation method of feed for improving egg laying performance and oviduct development of laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: 29Kg of rhizoma atractylodis, 22Kg of oldenlandia diffusa, 25Kg of liquorice, 11Kg of safflower and 6Kg of coix seed; pulverizing rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed, and sieving to obtain a basic raw material with the granularity of 400-500 mu m;
(2) 350g of lactic acid bacteria and 250g of saccharomycetes are taken and dissolved in 98.1Kg of water, 4.5Kg of glucose is added, and the mixture is activated for 4 hours at 35 ℃; the method comprises the steps of carrying out a first treatment on the surface of the
(3) Mixing the solution obtained in the step (2) with the solution obtained in the step (2)Mixing the basic raw materials obtained in the step (1) to ensure that the concentration of viable bacteria reaches 10 5 Above CFU/g, then ferment, ferment condition is: the temperature is 38 ℃, the moisture content is 50 percent, and the initial pH value is 7.0; the total number of viable bacteria in the fermentation material reaches 3.9x10 6 And (3) stopping fermenting when the pH value reaches 6.8 above CFU/g to obtain a fermented material.
(4) The fermented material is mixed with the hop powder in a mixing ratio of 6:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product reaches between 0.53% to obtain the feed. The beer pollen crushed material is obtained by crushing hops to 100 meshes.
Example 8
A preparation method of feed for improving egg laying performance and oviduct development of laying hens comprises the following steps:
(1) Weighing the following basic raw materials in weight: 28Kg of rhizoma atractylodis, 28Kg of oldenlandia diffusa, 21Kg of liquorice, 12Kg of safflower and 8Kg of coix seed; pulverizing rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed, and sieving to obtain a basic raw material with the granularity of 400-500 mu m;
(2) Uniformly mixing 42Kg of lactobacillus liquid and 30Kg of saccharomycete liquid;
(3) Mixing the solution obtained in the step (2) with the basic raw material obtained in the step (1) to ensure that the concentration of viable bacteria reaches 10 5 Above CFU/g, then ferment, ferment condition is: the temperature is 42 ℃, the moisture content is 26.4 percent, and the initial pH value is 7.0; the total number of viable bacteria in the fermentation material reaches 2.8x10 6 And (3) stopping fermenting when the pH value reaches 4.5 and obtaining the fermented material.
(4) The fermented material is mixed with the hop powder in a mixing ratio of 7:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product reaches between 0.78% to obtain the feed. The beer pollen crushed material is obtained by crushing hops to 60 meshes.
While only the preferred embodiments of the present invention have been described above, the scope of the present invention is not limited thereto, and it should be noted that equivalents and modifications, variations and improvements made according to the technical solution of the present invention and the inventive concept thereof, as well as those skilled in the art, should be considered as the scope of the present invention, without departing from the general inventive concept thereof.

Claims (8)

1. A feed for improving egg laying performance and oviduct development of laying hens is characterized in that: adding lactobacillus and saccharomycetes into the basic raw materials for mixed fermentation, wherein the total number of viable bacteria in the fermented materials reaches 10 5 The pH value reaches 3.0-6.8 above CFU/g, the fermented material is mixed with the hop crushed material, and the mixing ratio is 6:1-8:1 by weight, so that the total content of beta-acid and alpha-acid in the mixed product reaches 0.5-1%; the traditional Chinese medicine composition comprises, by weight, 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed.
2. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 1, wherein the method comprises the following steps of: the method comprises the following steps:
(1) Weighing the following basic raw materials in weight: 20-30 parts of rhizoma atractylodis, 20-30 parts of oldenlandia diffusa, 20-30 parts of liquorice, 10-15 parts of safflower and 5-10 parts of coix seed;
(2) Mixing lactobacillus and yeast fermentation broth or dissolving lactobacillus and yeast powder in water/nutrient solution for activation;
(3) Mixing the solution obtained in the step (2) with a basic raw material, and then fermenting;
(4) Mixing the fermented material with the hop powder to obtain the feed.
3. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 2, wherein the method comprises the following steps of: in the step (1), the rhizoma atractylodis, the oldenlandia diffusa, the liquorice, the safflower and the coix seed are crushed, sieved and fermented.
4. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 3, wherein the method comprises the following steps of: pulverizing rhizoma atractylodis, oldenlandia diffusa, liquorice, safflower and coix seed to 400-500 mu m, and then fermenting.
5. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 2, wherein the method comprises the following steps of: the fermentation conditions are as follows: the temperature is 18-45 ℃, the moisture content is 20-50%, and the initial pH value is 5.5-8.0.
6. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 2, wherein the method comprises the following steps of: and (3) spraying the solution obtained in the step (2) onto the basic raw materials.
7. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 2, wherein the method comprises the following steps of: inoculating, and ensuring that the concentration of viable bacteria reaches 10 5 CFU/g or more.
8. The method for preparing the feed for improving egg laying performance and oviduct development of the laying hens according to claim 2, wherein the method comprises the following steps of: the beer pollen crushed material is obtained by crushing hops to 60-100 meshes.
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