CN107183398A - A kind of feed for improving chicken egg productivity and preparation method thereof - Google Patents
A kind of feed for improving chicken egg productivity and preparation method thereof Download PDFInfo
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- CN107183398A CN107183398A CN201710291357.3A CN201710291357A CN107183398A CN 107183398 A CN107183398 A CN 107183398A CN 201710291357 A CN201710291357 A CN 201710291357A CN 107183398 A CN107183398 A CN 107183398A
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- powder
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
Abstract
The present invention relates to a kind of feed for improving chicken egg productivity and preparation method thereof, the feed is made up of the raw material of following parts by weight by boiling, crushing, mixing, fermentation：25~35 parts of monkshood, 25~35 parts of radix glycyrrhizae, 15~20 parts of rhizoma zingiberis, 3~5 parts of tealeaves, 25~30 parts of meat bone, 1~1.5 part of Bacillus acidi lactici bacterium solution, 0.5~1 part of kluyveromyces marxianus bacterium solution.The feed that the present invention is provided can not only effectively improve egg yield, improve the mouthfeel of egg, while improving the immunity of chicken, and with pure natural property, have no drug resistance, toxic side effect is small.
Description
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of feed of raising chicken egg productivity and its preparation side
Method.
Background technology
Nowadays, also there are many deficiencies during layer breeding, it is impossible to fully play the productive potentialities of laying hen,
Economic benefit is caused recessive drain occur.When particularly in the winter time, the egg production of chicken is decreased obviously.So, invention one kind promotes
Chicken is in cold winter it is also ensured that the feed of good egg production is imperative.
At present, in order to improve the laying rate of chicken and reduce chicken illness rate, various auxin, antibiosis are often added in feed
Element, hormone etc..Although improving the laying rate of laying hen, mouthfeel and nutritive value can not show a candle to the egg of family's poultry, and egg
Yellow partially white, egg white is thin, and storage time is short perishable.Meanwhile, the abuse of antibiotic easily causes livestock and poultry autogenous infection or two
Double infection contaminates, and long-term use is also easy to produce drug resistance.
In view of the above-mentioned problems, we have invented can not only effectively improve egg yield, improving the mouthfeel of egg, simultaneously
The immunity of chicken is improved, and with pure natural property, is had no drug resistance, the small feed of toxic side effect.
The content of the invention
In order to solve the mouthfeel for the reduction egg that existing feed is present, make the problem of chicken generates drug resistance, the present invention is carried
A kind of feed for improving chicken egg productivity and preparation method thereof has been supplied, egg yield can not only be effectively improved, has improved egg
Mouthfeel, while improving the immunity of chicken, and with pure natural property, has no drug resistance, the advantages of toxic side effect is small.The present invention will be solved
Technical problem certainly is achieved through the following technical solutions：
A kind of feed for improving chicken egg productivity, is prepared from the following raw materials in parts by weight：25~35 parts of monkshood, radix glycyrrhizae 25~
35 parts, 15~20 parts of rhizoma zingiberis, 3~5 parts of tealeaves, 25~30 parts of meat bone, 1~1.5 part of Bacillus acidi lactici bacterium solution, Marx's Crewe dimension ferment
Female 0.5~1 part of bacterium solution.
Further, above-mentioned monkshood is monkshood cauline leaf or fibrous root, and the radix glycyrrhizae is haulm of liquorice.
Further, the preparation method of above-mentioned Bacillus acidi lactici bacterium solution is：Bacillus acidi lactici is cultivated in MRS culture mediums, extremely
Exponential phase 0.6 × 108CFU/mL~1.0 × 108CFU/mL。
Further, the preparation method of above-mentioned kluyveromyces marxianus bacterium solution is：Kluyveromyces marxianus are existed
Cultivated in YPDA culture mediums, to exponential phase 0.6 × 108CFU/mL~1.0 × 108CFU/mL。
The preparation method of above-mentioned feed, comprises the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then 3~5h of boiling dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 80~120 mesh sieves, obtain rhizoma zingiberis
Powder, tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
25~35 parts of monkshood powder, 25~35 parts of radix glycyrrhizae powder, 15~20 parts of rhizoma zingiberis powder and 3~5 parts of tea are weighed respectively
Leaf powder, adds mixer, is stirred until homogeneous, obtains mixture A；
Step 4：Fermentation：
Mixture A is added to the mixing of 1~1.5 part of Bacillus acidi lactici bacterium solution and 0.5~1 part of kluyveromyces marxianus bacterium solution
In bacterium solution, the mass ratio for making Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, it is close
Fermentation is closed, tunning is obtained, tunning is freeze-dried, fermentate B is produced；
Step 5：Mix again：
Fermentate B and 25~30 parts of meat bone powder are well mixed, feed product is obtained.
Further, mixing speed is 1000~3000r/min in above-mentioned steps three, and mixing time is 10~15min.
Further, the temperature fermented in above-mentioned steps four is 37 DEG C~43 DEG C, and the time of fermentation is 15~20 days.
Compared with prior art, beneficial effects of the present invention：
1. monkshood, rhizoma zingiberis, radix glycyrrhizae can strengthen the immunity of chicken, chicken be improved to cold resistance, along with tealeaves
The mushroom of the meat meal tankage and high protein of tealeaves glycosides and high calcium and high nutrition, improves the egg production of chicken, improves the quality of egg.
2. by the fermentation of lactic acid bacteria and kluyveromyces marxianus, each material performance can be made to be harmonious, strengthen its effect,
Oxygen is taken by force using saccharomycete biology simultaneously, promotes lactic acid bacteria fast-growth to breed lactic acid producing and promotes the generation of fermentating metabolism product,
Suppress the growth of other miscellaneous bacterias, effectively improve the quality of feed, reduce the loss of active principle in feed, improve suitable
Mouth property and digestibility.
3. added in the present invention meat meal tankage and yeast be in order to cover its feed medium-height grass flavour of a drug so that mouthfeel more preferably,
Fragrance is more sufficient.
Brief description of the drawings
Fig. 1 and Fig. 2 are that the embodiment of the present invention 2 is fermented front and rear active ingredient comparative result.
Embodiment
Further detailed description is done to the present invention with reference to specific embodiment, but embodiments of the present invention are not limited to
This.
Embodiment 1：
A kind of feed for improving chicken egg productivity, is prepared from the following raw materials in parts by weight：It is 26 parts of monkshood, 35 parts of radix glycyrrhizae, dry
20 parts of ginger, 4 parts of tealeaves, 25 parts of meat bone, 1.5 parts of Bacillus acidi lactici bacterium solution, 0.5 part of kluyveromyces marxianus bacterium solution.
The preparation method of the feed, comprises the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then boiling 4h dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 120 mesh sieves, obtain rhizoma zingiberis powder,
Tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
26 parts of monkshood powder, 35 parts of radix glycyrrhizae powder, 20 parts of rhizoma zingiberis powder and 4 parts of tea-leaf powers are weighed respectively, add stirring
Machine, mixing speed is 3000r/min, and mixing time is 10min, obtains mixture A；
Step 4：Fermentation：
Mixture A is added to the mixed bacteria liquid of 1.5 parts of Bacillus acidi lactici bacterium solutions and 0.5 part of kluyveromyces marxianus bacterium solution
In, the mass ratio for making Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, at 43 DEG C
Sealed fermenting 15 days, obtains tunning, tunning is freeze-dried, and produces fermentate B；
Step 5：Mix again：
Fermentate B and 25 parts of meat bone powder are well mixed, feed product is obtained.
Embodiment 2：
A kind of feed for improving chicken egg productivity, is mainly prepared from the following raw materials in parts by weight：25 parts of monkshood, radix glycyrrhizae 25
Part, 15 parts of rhizoma zingiberis, 5 parts of tealeaves, 30 parts of meat bone, 1.2 parts of Bacillus acidi lactici bacterium solution, 0.8 part of kluyveromyces marxianus bacterium solution.
The preparation method of the feed, comprises the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then boiling 5h dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 120 mesh sieves, obtain rhizoma zingiberis powder,
Tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
25 parts of monkshood powder, 25 parts of radix glycyrrhizae powder, 15 parts of rhizoma zingiberis powder and 5 parts of tea-leaf powers are weighed respectively, add stirring
Machine, mixing speed is 1000r/min, and mixing time is 15min, obtains mixture A；
Step 4：Fermentation：
Mixture A is added to the mixed bacteria liquid of 1.2 parts of Bacillus acidi lactici bacterium solutions and 0.8 part of kluyveromyces marxianus bacterium solution
In, the mass ratio for making Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, at 40 DEG C
Sealed fermenting 20 days, obtains tunning, tunning is freeze-dried, and produces fermentate B；
Step 5：Mix again：
Fermentate B and 30 parts of meat bone powder are well mixed, feed product is obtained.
Embodiment 3：
A kind of feed for improving chicken egg productivity, is mainly prepared from the following raw materials in parts by weight：35 parts of monkshood, radix glycyrrhizae 30
Part, 18 parts of rhizoma zingiberis, 3 parts of tealeaves, 26 parts of meat bone, 1 part of Bacillus acidi lactici bacterium solution, 1 part of kluyveromyces marxianus bacterium solution.
The preparation method of the feed, comprises the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then boiling 3h dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 100 mesh sieves, obtain rhizoma zingiberis powder,
Tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
35 parts of monkshood powder, 30 parts of radix glycyrrhizae powder, 18 parts of rhizoma zingiberis powder and 3 parts of tea-leaf powers are weighed respectively, add stirring
Machine, mixing speed is 2000r/min, and mixing time is 12min, obtains mixture A；
Step 4：Fermentation：
In the mixed bacteria liquid that mixture A is added to 1 part of Bacillus acidi lactici bacterium solution and 1 part of kluyveromyces marxianus bacterium solution, make
The mass ratio of Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, in 37 DEG C of closed hairs
Ferment 20 days, obtains tunning, tunning is freeze-dried, and produces fermentate B；
Step 5：Mix again：
Fermentate B and 26 parts of meat bone powder are well mixed, feed product is obtained.
Embodiment 4：
A kind of feed for improving chicken egg productivity, is mainly prepared from the following raw materials in parts by weight：25 parts of monkshood, radix glycyrrhizae 25
Part, 15 parts of rhizoma zingiberis, 5 parts of tealeaves, 30 parts of meat bone, 2 parts of Bacillus acidi lactici bacterium solution.
The preparation method of the feed, comprises the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then boiling 5h dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 120 mesh sieves, obtain rhizoma zingiberis powder,
Tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
25 parts of monkshood powder, 25 parts of radix glycyrrhizae powder, 15 parts of rhizoma zingiberis powder and 5 parts of tea-leaf powers are weighed respectively, add stirring
Machine, mixing speed is 1000r/min, and mixing time is 15min, obtains mixture A；
Step 4：Fermentation：
Mixture A is added to the mixed bacteria liquid of 1.2 parts of Bacillus acidi lactici bacterium solutions and 0.8 part of kluyveromyces marxianus bacterium solution
In, the mass ratio for making Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, at 40 DEG C
Sealed fermenting 20 days, obtains tunning, tunning is freeze-dried, and produces fermentate B；
Step 5：Mix again：
Fermentate B and 30 parts of meat bone powder are well mixed, feed product is obtained.
Embodiment 4 is the contrast test of embodiment 2, and the difference with embodiment 2 is：Embodiment 4 uses 2 parts of Bacillus acidi lactici bacterium
Liquid, embodiment 2 uses 1.2 parts of Bacillus acidi lactici bacterium solution, 0.8 part of kluyveromyces marxianus bacterium solution, other conditions with reality
Apply example 2 identical.
Embodiment 5：
A kind of feed for improving chicken egg productivity, is mainly prepared from the following raw materials in parts by weight：25 parts of monkshood, radix glycyrrhizae 25
Part, 15 parts of rhizoma zingiberis, 5 parts of tealeaves, 30 parts of meat bone, 2 parts of kluyveromyces marxianus bacterium solution.
The preparation method of the feed, comprises the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then boiling 5h dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 120 mesh sieves, obtain rhizoma zingiberis powder,
Tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
25 parts of monkshood powder, 25 parts of radix glycyrrhizae powder, 15 parts of rhizoma zingiberis powder and 5 parts of tea-leaf powers are weighed respectively, add stirring
Machine, mixing speed is 1000r/min, and mixing time is 15min, obtains mixture A；
Step 4：Fermentation：
Mixture A is added to the mixed bacteria liquid of 1.2 parts of Bacillus acidi lactici bacterium solutions and 0.8 part of kluyveromyces marxianus bacterium solution
In, the mass ratio for making Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, at 40 DEG C
Sealed fermenting 20 days, obtains tunning, tunning is freeze-dried, and produces fermentate B；
Step 5：Mix again：
Fermentate B and 30 parts of meat bone powder are well mixed, feed product is obtained.
Embodiment 5 is another contrast test of embodiment 2, and the difference with embodiment 2 is：Embodiment 4 uses 2 parts of Marxs
Kluyveromyces bacterium solution, embodiment 2 uses 1.2 parts of Bacillus acidi lactici bacterium solution, 0.8 part of kluyveromyces marxianus bacterium solution, its
His condition is same as Example 2.
In embodiment 1-5, monkshood is monkshood cauline leaf or fibrous root, and radix glycyrrhizae is haulm of liquorice.The preparation of Bacillus acidi lactici bacterium solution
Method is：Bacillus acidi lactici is cultivated in MRS culture mediums, to exponential phase 0.6 × 108CFU/mL~1.0 × 108CFU/mL。
The preparation method of kluyveromyces marxianus bacterium solution is：Kluyveromyces marxianus are cultivated in YPDA culture mediums, to logarithm
Growth period 0.6 × 108CFU/mL~1.0 × 108CFU/mL。
Embodiment 1-5 performance study is as follows：
First, before and after fermenting, active ingredient compares：
1st, experimental method
(1) effective liquorice assay method
The preparation of reference substance solution：
Extracting liquorice glycosides reference substance, ammonium glycyrrhetate reference substance are appropriate, accurately weighed, plus 70% ethanol is respectively prepared every lmL and contained
Liquiritin 20mg, ammonium glycyrrhetate 0.2mg solution, produce reference substance solution.Glycyrrhizic acid weight=ammonium glycyrrhetate weight/
1.0207。
The preparation of need testing solution：
This product powder (crossing No. three sieves) about 0.2g is taken, it is accurately weighed, it is placed in the conical flask with plug, precision is added
70% ethanol 100mL, close plug, weighed weight, ultrasonically treated (power 250W, frequency 40kHz) 30 minutes is let cool, then weighed heavy
Amount, the weight of less loss is supplied with 70% ethanol, is shaken up, and is filtered, is taken filtrate, produce need testing solution.
Determination method：It is accurate respectively to draw reference substance solution and each l0 μ L of need testing solution, liquid chromatograph is injected, is determined,
The active ingredient of radix glycyrrhizae can be obtained.
(2) monkshood active ingredient and toxicity test method
The preparation of reference substance solution：
Take mesaconine reference substance, Hypaconitine reference substance, aconitine reference substance, benzoylmesaconine reference substance,
Benzoyl aconine reference substance, benzoyl time aconine reference substance are appropriate, accurately weighed, plus isopropanol-dichloromethane (1：
1) mixed solution is made, and 10 μ g mixed solution is respectively contained per lmL, reference substance solution is produced.
The preparation of need testing solution：
This product powder (crossing No. three sieves) about 2g is taken, it is accurately weighed, it is placed in the conical flask with plug, ammonification test solution 3mL,
Precision adds isopropanol-ethyl acetate (1：1) mixed solution 50mL, weighed weight, ultrasonically treated (power 300W, frequency
40kHz, water temperature is below 25 DEG C) 30 minutes, let cool, then weighed weight, with isopropanol-ethyl acetate (1：1) mixed solution is mended
The weight of sufficient less loss, shakes up, filtration.Precision measures filtrate 25mL, and less than 40 DEG C are recovered under reduced pressure solvent to dry, residue precision addition
Isopropanol-dichloromethane (1：1) mixed solution 3mL dissolves, filtration, takes filtrate, produces need testing solution.
Determination method：It is accurate respectively to draw reference substance solution and test solution each 10 μ L note people's liquid chromatographs, determine, can obtain
Monkshood active ingredient and toxicity.
(3) 6-gingerol content assaying method
The preparation of reference substance solution：
Take 6-gingerol reference substance appropriate, it is accurately weighed, plus solution of every lmL containing 0.lmg is made in methanol, produces reference substance
Solution.
The preparation of need testing solution：
This product powder about 0.25g is taken, it is accurately weighed, it is placed in the conical flask with plug, precision plus the methanol of people 75%
20mL, weighed weight, ultrasonically treated (power 100W, frequency 40kHz) 40 minutes is let cool, then weighed weight, is mended with 75% methanol
The weight of sufficient less loss, shakes up, filtration, takes filtrate, produces need testing solution.
Determination method：It is accurate respectively to draw reference substance solution and each 10 μ L of need testing solution, liquid chromatograph is injected, is determined,
6-gingerol content can be obtained.
(4) catechin, epicatechin content assaying method
The preparation of reference substance solution：
Catechin reference substance, epicatechin reference substance are taken, it is accurately weighed, plus methanol-water (1：1) mixed solution is respectively prepared
Per lmL 0.15mg containing catechin, epicatechin 0.lmg solution, reference substance solution is produced.
The preparation of need testing solution：
This product fine powder about 20mg is taken, it is accurately weighed, put in 50mL measuring bottles, plus methanol-water (1：1) mixed solution 40mL, surpasses
Sonication 20 minutes, and add methanol-water (1：1) mixed solution shakes up to scale, filtration, takes filtrate, produces need testing solution.
Determination method：It is accurate respectively to draw above two reference substance solution and each 5 μ L of need testing solution, inject liquid chromatogram
Instrument, determines, can obtain catechin, epicatechin content.
(5) measure of lactic acid content
The drafting of standard curve：
The μ gmL of lactate standard liquid 1,2,3,4,5,6 are respectively configured-1, be separately added into colorimetric cylinder lactate standard liquid 1mL,
4.0% copper-bath 0.05mL, adds concentrated sulfuric acid 6mL, reacts 5min, is cooled to after less than 20 DEG C, then respectively at each ratio
1% alkali solubility xenol solution 0.05mL is added in colour tube, is well mixed, 6-8h is placed at room temperature, overnight, in 570nm
Lower carry out colorimetric estimation, draws out standard curve.
Sample is determined：1g fermented feeds are dissolved in 100mL distilled water, 4 000rpm centrifugation 10min, take supernatant 1mL,
By lactic acid content (%) in standard curve determination sample.
(6) measure of oligomeric polysaccharide content
The preparation of reference substance solution：
Mannose, rhamnose, glucose, galactolipin, arabinose, xylose standard product 0.5g accurately are weighed, 50% second is used
Alcoholic solution dissolves and is settled to 10mL, is configured to the standard reserving solution that concentration is 50mg/mL, produces reference substance solution.
The preparation of need testing solution：
Feed Sample 1g is weighed in 100mL beaker, 25mL50% ethanol solutions are added, surpassed in ultrasonic oscillator
After sound extraction 20min, it is transferred in 50mL volumetric flasks, 50% ethanol solution is settled to scale, mixes, supernatant is taken, with 0.45 μ
M membrane filtrations, filtrate is for analysis.
Determination method：It is accurate respectively to draw reference substance solution and each 20 μ L of need testing solution, liquid chromatograph is injected, is determined,
Oligomeric polysaccharide content can be obtained.
(7) measure of protein content
Sample treatment：
1g samples are weighed, accurately to 0.0002g, are put into kjeldahl flask, copper sulphate 0.4g, anhydrous potassium sulfate 6g is added,
Selenium powder 0.004g, with sample mixed, then enriching sulfuric acid 12.5mL, carefully heats on stove disappearing to boil, treats sample coking, foam disappears
Lose, strengthen firepower, to solution clarification, reheat at least 1h.The solution after boiling that will disappear is cooled down, and 100mL appearances are transferred to without loss
In measuring bottle, constant volume is sample decomposed solution after cooling, takes the boric acid solutions of 20mL 2%, plus mixed indicator 2 to drip, distills semimicro
This solution is immersed in the end of device, accurately pipettes in sample decomposed solution 10mL injection reative cells, is stoppered entrance glass stopper, adds
10mL 40%NaOH solution, carefully lifts glass stopper and is allowed to flow into reative cell, is stoppered glass stopper, and the sealing that adds water in porch
It is good, gas leakage is prevented, is distilled, self-absorption liquid is changed into blueness and starts timing, after 4 minutes, condensation pipe end is left absorption liquid level, then
Distillation 1 minute, condensation pipe end is cleaned with distilled water, and washing lotion flows into absorbing liquid.
Titration：Absorbing liquid after absorbing ammonia is titrated with 0.02mol/L standard liquid immediately, and it is red that solution becomes ash by blueness
Color is terminal, and record consumes the volume of standard liquid, calculates protein content.
2nd, experimental result
Embodiment 1 is fermented front and rear active ingredient comparative result such as Fig. 1 and Fig. 2, in figure, and " * " is represented compared with control group
Experimental group has significant difference in P ＜ 0.05, and " * * " represent that experimental group has pole significant difference in P ＜ 0.01 compared with control group.
Fermentation can preserve feed and its nutriment for a long time, meanwhile, the activity of microorganism makes ensilage with fragrance
Sour-sweet taste, improves the palatability of domestic animal.Also, fermentation can make diester-type alkaloids (mesaconine, the secondary crow of monkshood
Head alkali, aconitine) monoester alkaloid (benzoyl mesaconine, benzoyl Hypaconitine, benzoyl aconite alkali) is converted into, from
And the toxicity of monkshood is reduced, and fermenting can make effect of monkshood and radix glycyrrhizae mutually merge auxiliary.What fermented tcm had
Advantage：(1) content of effective ingredient is fully improved；(2) attenuation synergistic is played a part of；(3) pharmaceutical molecular weight is reduced.
Active ingredient comparative result before and after fermentation：
As can be seen from Figure 1：Compared before and after fermentation, diester-type alkaloids (mesaconine, Hypaconitine, aconitine) content is bright
Aobvious reduction, monoester alkaloid (benzoylmesaconine, benzoyl aconine, benzoyl time aconine), xylose contain
Measure showed increased.
As can be seen from Figure 2：Compared before and after fermentation, lactic acid, protein, liquiritin, catechin, epicatechin content are all obvious
Increase, wherein, lactic acid, protein content have pole significant difference.
2nd, formula trial effect compares
1st, influence of the DIFFERENT FEED to laying rate
Choose 900 builds uniform, vitality is good, kind is identical, the laying hens of 160 ages in days is as experimental subjects, at random
It is divided into 6 groups, every group 150, respectively tests 1 group, test 2 groups, test 3 groups, test 4 groups, experiment 5 groups and control group.Experiment
Period, feed in 1 group of edible embodiment 1 is tested, test feed in 2 groups of edible embodiments 2, tested and raised in 3 groups of edible embodiments 3
Material, tests the feed in 4 groups of edible embodiments 4, tests feed in 5 groups of edible embodiments 5, and control group is bought using common market
Feed (main component is：1 part of vitamin a, 1 part of vitamin b, a vitamin e, 1 part of nicotinic acid, 1 part of pantothenic acid, 1 part of folic acid, 1
Part Choline Chloride, 2 parts of salt, 1 part of methionine, 22 parts of soya-bean cakes, 8 parts of stone flours, 60 parts of corncobs), the number of times that feeds intake daily, feed intake
The all sames such as amount, charging time, Continuous Observation 4 months (this experiment is to 2 months 2017 in November, 2016) calculates average day
Egg production, the results are shown in Table 1.
Table 1
| Laying rate % | Egg size (g/) | Feed intake [g/ (* days)] | Feedstuff-egg ratio | |
| Test 1 group | 94%Ab | 68.3±0.53a | 125.76±1.86a | 1.959b |
| Test 2 groups | 97%Aa | 68.2±0.49a | 126.12±1.72a | 1.906b |
| Test 3 groups | 93%Ab | 67.5±0.41a | 125.63±1.69a | 2.001b |
| Test 4 groups | 88%Bc | 67.3±0.48a | 124.56±1.98a | 2.103b |
| Test 5 groups | 84%Bc | 66.8±0.56a | 124.13±2.12a | 2.212b |
| Control group | 75%Cd | 64.9±0.51b | 123.91±2.12a | 2.546a |
In table 1, the different lowercase letter indication differences of colleague's data shoulder mark are significantly (P ＜ 0.05)；Shoulder mark difference capitalization
Represent difference extremely significantly (P ＜ 0.01)；Shoulder mark same letter represents difference not significantly (P ＞ 0.05) without letter.
2nd, influence of the different formulations feed to chicken immunity
In just examination morning the 120th day phase, collection test chicken venous blood 5mL, stands 30min, treats serum at room temperature on an empty stomach
After precipitation, it is stand-by that blood serum sample is made in low temperature 3000r/min centrifugation 15min.Using kit measurement total protein (BCA methods), in vain
Albumen (Bromocresol green), glutamic-pyruvic transaminase/glutamic-oxalacetic transaminease (reitman-frankel method)；RNA isolation kit determines immunoglobulin A, G, M
(IgA, IgG, IgM), TAC, total number born and concentration of malondialdehyde.All operations are in strict accordance with examination
Agent box operation instructions are carried out.Experimental result such as table 2 and table 3.
Influence of the different formulations feed of table 2 to chicken serum immunologic function parameter
In table, the different lowercase letter indication differences of colleague's data shoulder mark are significantly (P ＜ 0.05)；Shoulder mark difference capital letter matrix
Show difference extremely significantly (P ＜ 0.01)；Shoulder mark same letter represents difference not significantly (P ＞ 0.05) without letter.
Influence of the different formulations feed of table 3 to the anti-oxidant parameter of chicken serum
In table, the different lowercase letter indication differences of colleague's data shoulder mark are significantly (P ＜ 0.05)；Shoulder mark difference capital letter matrix
Show difference extremely significantly (P ＜ 0.01)；Shoulder mark same letter represents difference not significantly (P ＞ 0.05) without letter.
Total protein content has reacted Absorption of the body to albumen, and the relation with humoral immunity in serum.White egg
It is the transport agent of water-soluble relatively low material in the main body and blood for constitute plasma colloid osmotic pressure in vain, to liver in protein metabolism
In play an important role.Glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease are the important symbols for reflecting liver and cardiac function, if active
It is too high, illustrate that liver and heart are possible to be compromised.Immunoglobulin is that the mankind and higher mammal are produced in vivo by after antigenic stimulus
Raw class protein that can be with antigen generation specific effect, also known as antibody, play an important roll to immunity of organisms, are immunized
The height of level reflects resistivity of the body to disease indirectly.TAC is to weigh body Function of Antioxidant System
The composite target of situation, can represent and reflect body enzyme and the comprehensive effect and body free radical of non-enzyme Antioxidation Mechanism
Metabolism status, can be used to evaluate the height of oxidation resistance.
As can be known from Table 2：The chicken that embodiment 2 is formulated prepared feed is eaten, with eating embodiment 1, embodiment 3, embodiment
4th, embodiment 5, the chicken of feed prepared by control group of formula are compared, total protein, albumin, IgA, IgG, IgM content in serum
At most, glutamic-oxalacetic transaminease, glutamic-pyruvic transaminase content are minimum, illustrate that embodiment 2 is formulated matched somebody with somebody feed to improving chicken immunity effect
Fruit is optimal.
The chicken that embodiment 1, embodiment 3 are formulated prepared feed is eaten, with eating embodiment 4, embodiment 5, compareing group of formula
The chicken of prepared feed is compared, and total protein, albumin, IgA, IgG, IgM content are more in serum, glutamic-oxalacetic transaminease, Gu Bingzhuan
Ammonia enzyme content is low, illustrates that embodiment 1, embodiment 3 are formulated matched somebody with somebody feed ratio embodiment 4, embodiment 5, control group of formula made
Standby feed is good to improving chicken immunity effect.
Eat the chicken that embodiment 4, embodiment 5 are formulated prepared feed, the chicken phase with compareing the feed prepared by group of formula
Than total protein, albumin, IgA, IgG, IgM content are more in serum, and glutamic-oxalacetic transaminease, glutamic-pyruvic transaminase content are low, illustrate to implement
The feed that example 4, embodiment 5 are formulated prepared by matched somebody with somebody feed ratio control group of formula is good to improving chicken immunity effect.
As can be known from Table 3：The chicken that embodiment 2 is formulated prepared feed is eaten, with eating embodiment 1, embodiment 3, embodiment
4th, embodiment 5, the chicken of feed prepared by control group of formula are compared, and TAC, total Superoxide dismutase ability be most in serum
By force, mda content is minimum, illustrates that case study on implementation 2 is formulated matched somebody with somebody feed to improving chicken immunity best results.
The chicken that embodiment 1, embodiment 3 are formulated prepared feed is eaten, with eating embodiment 4, embodiment 5, compareing group of formula
The chicken of prepared feed is compared, and TAC, total Superoxide dismutase ability are strong in serum, and mda content is low, illustrates real
The matched somebody with somebody feed ratio embodiment 4 of example 1, the formula of embodiment 3, embodiment 5, the feed compareed prepared by group of formula is applied to improving chicken to exempt from
Epidemic disease power effect is good.
Eat the chicken that embodiment 4, embodiment 5 are formulated prepared feed, the chicken phase with compareing the feed prepared by group of formula
Than TAC, total Superoxide dismutase ability are strong in serum, and mda content is low, illustrate embodiment 4, the formula of embodiment 5 institute
The feed prepared by feed ratio control group of formula matched somebody with somebody is good to improving chicken immunity effect.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (7)
1. a kind of feed for improving chicken egg productivity, it is characterised in that：It is prepared from the following raw materials in parts by weight：Monkshood 25~35
Part, 25~35 parts of radix glycyrrhizae, 15~20 parts of rhizoma zingiberis, 3~5 parts of tealeaves, 25~30 parts of meat bone, 1~1.5 part of Bacillus acidi lactici bacterium solution, horse
Gram 0.5~1 part of this kluyveromyces bacterium solution.
2. feed as claimed in claim 1, it is characterised in that：The monkshood is monkshood cauline leaf or fibrous root, and the radix glycyrrhizae is
Haulm of liquorice.
3. feed as claimed in claim 1, it is characterised in that：The preparation method of the Bacillus acidi lactici bacterium solution is：By lactic acid bar
Bacterium is cultivated in MRS culture mediums, to exponential phase 0.6 × 108CFU/mL~1.0 × 108CFU/mL。
4. feed as claimed in claim 1, it is characterised in that：The preparation method of the kluyveromyces marxianus bacterium solution is：
Kluyveromyces marxianus are cultivated in YPDA culture mediums, to exponential phase 0.6 × 108CFU/mL~1.0 × 108CFU/
mL。
5. a kind of preparation method of the feed described in any one of Claims 1 to 4, it is characterised in that：Comprise the following steps：
Step one：Boiling：
Monkshood, radix glycyrrhizae are separately added into water, then 3~5h of boiling dries monkshood, radix glycyrrhizae respectively；
Step 2：Crush：
By rhizoma zingiberis, tealeaves, meat bone, dry after monkshood and radix glycyrrhizae crush respectively, cross 80~120 mesh sieves, obtain rhizoma zingiberis powder,
Tea-leaf power, bone and flesh powder, monkshood powder and radix glycyrrhizae powder；
Step 3：Mixing：
25~35 parts of monkshood powder, 25~35 parts of radix glycyrrhizae powder, 15~20 parts of rhizoma zingiberis powder and 3~5 parts of tea leaf powders are weighed respectively
End, adds mixer, is stirred until homogeneous, obtains mixture A；
Step 4：Fermentation：
Mixture A is added to the mixed bacteria liquid of 1~1.5 part of Bacillus acidi lactici bacterium solution and 0.5~1 part of kluyveromyces marxianus bacterium solution
In, the mass ratio for making Bacillus acidi lactici bacterium solution, the gross mass of kluyveromyces marxianus bacterium solution and mixture A is 1：49, closed hair
Ferment, obtains tunning, and tunning is freeze-dried, and produces fermentate B；
Step 5：Mix again：
Fermentate B and 25~30 parts of meat bone powder are well mixed, feed product is obtained.
6. preparation method as claimed in claim 5, it is characterised in that：Mixing speed is 1000~3000r/ in the step 3
Min, mixing time is 10~15min.
7. preparation method as claimed in claim 5, it is characterised in that：The temperature fermented in the step 4 is 37 DEG C~43
DEG C, the time of fermentation is 15~20 days.
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