CN104782959A - Feed for promoting development of ovary and fallopian tube of laying hen and preparation method of feed - Google Patents
Feed for promoting development of ovary and fallopian tube of laying hen and preparation method of feed Download PDFInfo
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Abstract
The invention discloses feed for promoting the development of the ovary and the fallopian tube of a laying hen and a preparation method of the feed, and belongs to the technical field of feed composition. The feed provided by the invention is prepared by scientifically adding plant extracts, corn bee pollen extracts, Chinese herbal medicine extracts, modified dietary fiber, one or more prebiotic factors and the like into early laying period chicken feed. Feeding tests show that compared with laying hens fed with two types of peak-laying feed sold in market, the laying hen fed with the feed provided by the invention has the advantages that the length of the fallopian tube is improved by 6.82 cm and 4.62 cm respectively; the weight of the fallopian tube is improved by 11.49 g and 7.46 g respectively; the weight of the ovary is improved by 6.08 g and 4.17 g respectively; the egg laying rate is improved by 2.07% and 2.15% respectively; the egg laying quantity is improved by 2.73 g and 1.92 g respectively; the shank length is improved by 7 mm and 5 mm respectively; the body weight is improved by 0.07 kg and 0.05 kg respectively; the survival rate is improved by 2.4% and 3.2% respectively. Therefore, the laying hen fed with the feed provided by the invention has higher reproductive performance, production performance, anti-immunity, and disease-resistant performance.
Description
Technical field
The invention belongs to fodder compound technical field, be specifically related to a kind ofly promote feed of laying hen ovary and Oviduct Development and preparation method thereof.
Background technology
The climbing material feed that to be exactly laying hen 5% laying rate use to peak phase of laying eggs (i.e. 5% laying rate-32 week age), this stage laying rate rises rapidly, and laying rate curve tendency as the cliffy summit, from top to bottom, rises rapidly.Laying hen body weight increases sharply simultaneously, and reproductive system is also grown rapidly, and the ovarian follicle on ovary grows in a large number fast, and fallopian tubal is rapid thicker elongated, weight increase also, and reproductive organs ovary and fallopian tubal are progressively tending towards ripe, substantially complete growth to during 34 week age.Body weight increases and reproductive system maturation is carried out simultaneously.At this moment part chicken starts to lay eggs, and supermature chicken group laying rate reached 3--5% 18 week age, and 22 week age reached 78%, and 25 week age reached 92%.So this, chicken all required very strict to the various nutrient in feed and external environmental condition in period.
At present, commodity egg feed brood time high strength immunity frequently in process, medication, disconnected beak etc. stress negative effect larger, nutrition supply is not considered the stress nutrition needs of this phase, the nutrition of daily ration of making to brood can not meet its actual needs, causes brood time body type indicators (shin long, body weight) not up to standard; Incubation diets of different nutrition level is low, raw materials used quality is generally poor, brood time is replaced with incubation daily ration by all ages later at once, and not according to body type indicators developmental state adjustment diet nutrient, makes original hypogenetic build not obtain growing compensation at finishing period; The expected date of childbirth (being bred as the later stage) is the laying hen reproductive organs rapid puberty, needs the pre-product material of higher trophic level, still uses low nutrition daily ration, have impact on the growth of ovary, fallopian tubal etc., make Development of Reproductive System insufficient in production.Early stage (climbing peak phase) of laying eggs is laying hen reproductive development whether key period of normal, sizing and the amortization period, and target effectively ensures that organ of multiplication reaches full growth, and opens product initial stage egg size large, and that lays eggs on peak is fast; Maximally increase body weight, ensure body deposit, make peak remain lasting, maximum to lifelong egg production.This stage needs comprehensively, the peak material of climbing of efficient, high-plane of nutrition, and the peak material but existing feeding pattern is not climbed, just started 18 week age laying hen material of feeding, and rising 20 week age feeds, and peak of laying eggs is expected; Or the omnidistance one that uses of laying eggs is expected, have impact on the normal development of ovary, fallopian tubal etc., makes Development of Reproductive System delayed.Have a strong impact on the normal development of laying hen build and reproductive organs, the problems such as cause the slow of peak life of laying eggs, egg size is little, and lifelong egg production is low, and death rate is high.
Along with the development of scale, intensive culture industry, various New Type of Diseases and nonstandard feeding and management seriously govern the development of aviculture.Cause the improper growth of ovary, fallopian tubal not only relevant with the low nutrition of feed, also relevant with factors such as laying hen immunity of organisms and premunitions, such as due to such as non typical newcastle disease, influenza, egg drop syndrome, biography prop up, colibacillosis, white diarrhea, cholera fowl, duck oromeningitis infection etc. can cause oaritis, salpingitis, and then cause laying rate to decline, often there is the phenomenon that soft-shelled egg, shell egg, the egg that fades, arenaceous shell egg, blood cake egg, lopsided egg increase simultaneously; For another because of Egg Production of Laying Hens too much, chicken body immunity declines, anti-fatigue ability declines, and also can cause ovary, fallopian tubal can not normal development, causes performance in layers to decline.
Therefore, the many factors such as the digestic property of the normal development of laying hen reproductive system and the nutritive peculiarity of each feeding period egg feedstuff, disease resistance, immunity and laying hen are relevant, lay eggs early stage (climbing peak phase) particularly important, need jointly to set about from above-mentioned many-side, to promote the full development of laying hen reproductive system.Application number be 201210060338.7 Chinese patent disclose a kind of climbing material improving commodity egg reproductive performance, with corn, wheat, corn protein powder, dregs of beans, peanut meal, probiotics, vitamin E, Organic Selenium, Organic Chromium and organic zinc etc. are primary raw material, the medium-and-large-sized chicken house laying hen climbing that is applicable to of adequate nutrition level design uses novel fodder period, effectively can promote that organ of multiplication is grown rapidly and maturation, reduce various stress reaction, guarantee that body maturation and sexal maturity are synchronously grown, laying hen rapidly on to lay eggs peak, improve egg size, improve egg production, solid foundation has been established for maintaining peak persistence.Above-mentioned disclosed patent just solves raising laying hen reproductive performance problem from climbing the single trophism aspect of peak phase feed diet, and effect is undesirable, and nutrition is not comprehensive yet.Application number be 201310019618.8 Chinese patent disclose a kind of compound premix promoting laying hen build and the development of ovary, this invention has been screened and laying hen bone meticulously, reproductive system grows the vitamin A of substantial connection with resisting stress, vitamine D3, vitamin E, copper, manganese, zinc and Chinese herbal medicines, according to the collaborative of them and the antagonism relationship, develop specific complex vitamin, composite trace element and Chinese herbal formula, on the basis of adding mentioned component, with the addition of oil powder especially, Choline Chloride, calcium monohydrogen phosphate, stone flour, salt, probiotic, phytase etc., by the meticulous collocation to above various raw material, this laying hen premix compound special can promote that laying hen grows and Development of Reproductive System, improve laying hen build, strengthen disease-resistant anti-stress ability, for laying period, the raising of production performance is laid a good foundation.The Chinese herbal medicine that Chinese herbal formula in above-mentioned publication adopts is not comprehensive, can not solve that laying hen is disease-resistant fully and effectively, heat resistanceheat resistant, anti-cold, anti-oxidant, improve immunity to promote the problem that reproductive organs (fallopian tubal, ovary) is grown, the simultaneously also unexposed method effectively utilizing Chinese herbal medicine effective ingredients, and, this compound premix be only applicable to laying period before each stage, be not suitable for early-stage fodder of laying eggs.The open defect of this invention is that the while of promoting that organ of multiplication is grown by premix, body weight increases too much, and 16 week age, body weight reached 1685 grams, and now within laying hen SBW 1400 grams.Little fat chicken is cultivated in the harm that now laying hen body weight is large, and egg size is large, suppresses egg, hen prolapse of the anus, causes reproductive performance low, seriously reduce laying rate.Application number be 201310301767.3 Chinese patent disclose a kind of feed addictive improving chicken egg productivity and preparation method thereof, mainly shattered by Chinese herbal medicine and obtain probiotics with fermentable, improve intestinal health to improve laying hen reproductive performance, its fermentation period is long, Chinese herbal medicine utilization rate is low, solve emphatically and promote laying hen reproductive development problem, the interpolation of the early-stage fodder that can not be applicable to very well to lay eggs, application number be 201410516014.9 Chinese patent disclose and a kind of there is the health-care Chinese medicinal composition and preparation method thereof strengthening performance in layers, mainly Chinese herbal medicine is shattered the digestibility and utilization directly promoting nutriment, by the integrated regulation of laying hen body self to improve reproductive endocrine system, accelerate to repair reproductive organs pathology, promote ovary, Oviduct Development, accelerate follicle maturity, be beneficial to and lay eggs, ensure and improve body production performance, but, its Chinese herbal medicine utilization rate is low, cost of material is high, effect is undesirable, can not be applicable to lay eggs the interpolation of early-stage fodder equally very well.
To sum up, according to physiology, the growth characteristics of Egg Production of Laying Hens early stage (climbing peak period), develop that a kind of comprehensive nutrition, effect are high, performance entirely, effectively promotes that the early-stage fodder of laying eggs that reproductive organs (fallopian tubal, ovary) is grown is extremely urgent.
Summary of the invention
Technical problem solved by the invention be overcome existing laying hen feed to climb peak phase (laying eggs early stage) early-stage fodder of not laying eggs in process or lay eggs early-stage fodder not science, not comprehensively, the defect such as weak effect, according to climbing the special physiology of peak phase laying hen, growth characteristics, climb peak period physiological requirements and nutritional need for Major Nutrient design considerations with laying hen; Efficiently to promote that laying hen reproductive organs (fallopian tubal, ovary) is grown for main target, composite plant extracts, bee pollen of maize extract, Chinese herbal medicine extract, modified dietary fiber, the prebiotic factor significantly improving laying hen reproductive ability, immunity, disease resistance, trophism and digestibility of science, prepares the feed of a kind of effective promotion laying hen ovary and Oviduct Development.
In order to achieve the above object, the present invention is by the following technical solutions:
Promote a feed for laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight:
Cereal 53-60 part, dregs of beans 5-20 part, modified dietary fiber 13-15 part, fermented bean dregs 3-8 part, corn byproducts 2-6 part, plant extracts 2-5 part, Chinese herbal medicine extract 2-5 part, cotton dregs 2 parts, rice bran meal 1-8 part, rice bran 1-8 part, stone flour 8-9 part, calcium monohydrogen phosphate 0.8 part, prebiotic factor 2-4 part, rapeseed dregs 0-3 part, bee pollen of maize extract 4-6 part, vegetable oil 1-3 part, amino acid 0.35-3 part, compound premix 0.5 part, complex enzyme 0.05-0.1 part, salt 0.2 ~ 0.4 part, Choline Chloride 0.05-0.12 part, probiotic 0.05-0.1 part, zinc methionine 0.01-0.07 part, phytase 0.01-0.03 part, Organic Chromium 0.001 ~ 0.003 part, Organic Selenium 0.001-0.003 part.
Further, to be corn or corn mix with any one in rice, wheat, Chinese sorghum described cereal.
Further, described modified dietary fiber is that the Soluble Fiber cellulose content obtained through physics, chemistry or biological method process by dietary fiber is high, biologically active strong, has cellulose that is important, positive role to laying hen beneficial bacteria of intestinal tract group, compared with full diet fiber, its biological action is more powerful;
Preferably, described modified fibre is obtained through biology enzyme enzymolysis by one or more in inulin, apple fiber, Semen Tritici aestivi fiber;
More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 5-7:4-6:3-5 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;
Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 7-9:3-5:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.
Further, described corn byproducts is one or more mixing in DDGS, corn germ cake, corn bran.
Further, described plant extracts is that soybean higher for isoflavone content, clover science is composite, adopts the low temperature extractive techniques such as ultrasonic cleaning, freezing, pulverizing, high-pressure pulse electric extraction, ultrasonic wave added Microwave Extraction and obtains;
Preferably, the preparation method of described plant extracts, comprise the following steps: northeast soybean and red clover are put into respectively the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed and clean 5-10min in 200W, 40KHz, drain, 5-9:3-6 Homogeneous phase mixing in mass ratio, then in-18--23 DEG C of freezing 5-10min; Be crushed to particle diameter 0.5-1.5mm immediately; To put in container and to add the benzinum of 10-15 times of weight, putting into water bath chader 35-45 DEG C of concussion 1-3h, suction filtration, drying to obtain mixed powder; Add mixed powder quality 15-25 60% ethanol doubly, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, carries out high-pressure pulse electric process under pulse frequency 200-300Hz condition; Then in 30-40 DEG C of insulation 1-2h, be then warming up to 40-50 DEG C under power 150-300W condition, carry out microwave irradiation extraction 15-20min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Extracting liquid filtering, obtains filtrate, filter residue 1-3 times of weight 45-60 DEG C of water rinse 3 times, and rinsing liquid and filtrate merge, Homogeneous phase mixing, and being evaporated to solid content is more than 15%, and namely freeze drying obtains plant extracts;
More preferably, described microwave irradiation is extracted as batch (-type) and extracts, i.e. microwave irradiation 10s, interval 20s;
In the plant extracts filtrate adopting said method to prepare, the extracted amount of isoflavones is 21.68-24.13mg/g, and crude extract yield is 33-36%, and crude extract purity is 3.82%.
Further, described Chinese herbal medicine extract significantly improves laying hen to resist hot and cold irritability, disease resistance and immunity; Guarantee that body health is strong, effectively overcome laying hen physiology apocleisis and the large weakness liking morbidity of physiological stress of upper peak phase, effectively increase laying hen fallopian tubal and ovary weight, accelerate follicle maturity; The Chinese herbal medicine accelerating nutrients deposition is raw material, prepares, effectively can prevent the generation of salpingitis and oaritis through ultrasonic cleaning, low temperature enzymolysis, water extraction alcohol extracting and ultrasonic assistant Microwave Extraction, improves the reproductive performance of laying hen comprehensively;
Preferably, the preparation method of described Chinese herbal medicine extract, comprises the steps: to count by weight, takes Radix Astragali 15-20 part, motherwort 15-20 part, barrenwort 13-18 part, hawthorn 12-15 part, dried orange peel 12-15 part, Radix Glycyrrhizae 10-15 part, rhizoma zingiberis 10-15 part, Ligusticum wallichii 8-12 part, tuber of multiflower knotweed 6-10 part, Radix Angelicae Sinensis 6-10 part, Radix Codonopsis 6-10 part, bark of eucommia 5-8 part, seed of Chinese dodder 3-7 part, fruit of glossy privet 3-7 part, oyster 3-5 part, giant knotweed 2-5 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is less than 2 millimeters, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70 DEG C-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, Microwave Extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the mixed enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter, obtain the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85 DEG C-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, filter vacuum concentrates postlyophilization, pulverizes and obtains Chinese herbal medicine extract;
Described mixed enzyme is dextranase, zytase, pentosanase, pectase 2-4:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.
Further, the described prebiotic factor is at least one in oligoisomaltose, FOS, stachyose, oligomeric maltose;
Preferably, described prebiotic factor percentage by weight consists of oligoisomaltose 30-40%, FOS 20-30%, stachyose 20-30%, oligomeric maltose 15-25%.
Further, described bee pollen of maize extract is with bee pollen of maize, corn stigma for raw material, successively through ultrasonic cleaning, high-pressure pulse electric and ultrasonic process, biological enzymolysis and obtained;
Preferably, the preparation method of described bee pollen of maize extract, comprise the steps: that 20-40:3-5 takes bee pollen of maize and corn stigma respectively in mass ratio, put in the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed, in 200W under room temperature, 40KHz cleans 3-5min, drain, put into container, add former bee pollen of maize quality 3-5 water doubly, be 4-6 by lactic acid adjust ph, in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 10-15min, be warming up to 40-50 DEG C, add the compound enzyme of former bee pollen of maize quality 3-5%, stir, enzymolysis 15-20min, enzymolysis liquid liquid filters through 100-300 eye mesh screen, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, reduced pressure concentration, freeze drying,
The quality group of described compound enzyme becomes: protease: pectase: cellulase: lipase=11-13:5-7:4-6:1-3.
Further, zytase, mannonase acid protease, lipase, amylase, dextranase is contained in described complex enzyme.
Further, described amino acid is the mixing of in lysine, methionine, threonine or tryptophan two kinds or more;
Preferably, described amino acid is lysine, methionine, threonine and tryptophan 1:0.8-1.2:0.5-1.0:0.2-0.5 Homogeneous phase mixing in mass ratio.
The preparation method of the feed of above-mentioned promotion laying hen ovary and Oviduct Development, comprises the following steps:
Accurately take each component raw material according to formula, all conveniently laying hen material processing method is pulverized, and then arrogantly by weight adds surplus stock successively, Homogeneous phase mixing to little, and processing, must promote the feed of laying hen ovary and Oviduct Development.
Beneficial effect:
The present invention, according to climbing the special physiology of peak phase laying hen, growth characteristics, climbs peak period physiological requirements and nutritional need for Major Nutrient design considerations with laying hen; Efficiently to promote that laying hen reproductive organs (fallopian tubal, ovary) normal development is for main target, composite plant extracts, bee pollen of maize extract, Chinese herbal medicine extract, modified dietary fiber, the prebiotic factor significantly improving laying hen reproductive ability, immunity, disease resistance, trophism and digestibility of science, prepares the feed of a kind of effective promotion laying hen ovary and Oviduct Development.Show through feeding experiment: compare with control group (market expects 1 and 2), of the present invention group of laying hen fallopian tubal length long 6.82cm and 4.62cm respectively, fallopian tubal weight fraction you can well imagine high 11.49g and 7.46g, and ovary weight improves 6.08g and 4.17g respectively, significant difference.The quantity of large yellow ovarian follicle many 2.2 and 1.8 respectively, little ovarian follicle quantity many 3.6 and 3.1 respectively, as can be seen here, of the present invention group has significant facilitation to laying hen upper peak fallopian tubal in early stage and the development of ovary; Of the present invention group of laying rate improves 2.07% and 2.15% respectively, and egg production improves 2.73 grams and 1.92 grams respectively, has good economic benefit; Of the present invention group of long long 7mm and 5mm respectively of shin, body weight improves 0.07kg and 0.05kg respectively, and body weight and build are obviously better than market group, and field observation of the present invention group of build is delicate and pretty compact, solid.Survival rate improves 2.4% and 3.2% respectively, demonstrates the stronger anti-immunity of laying hen and disease resistance.
Concrete know-why is as follows:
1. plant extracts of the present invention is that soybean higher for isoflavone content, clover science is composite, adopt the low temperature extractive techniques such as ultrasonic cleaning, freezing, pulverizing, high-pressure pulse electric extraction, ultrasonic wave added Microwave Extraction and obtain, simultaneously containing isoflavones and clover isoflavones, gained isoflavones recovery rate is high, purity is high, physiologically active is strong, in extract filtrate, the extracted amount of isoflavones is 21.68-24.13mg/g, crude extract yield is 33-36%, and crude extract purity is 3.82%.Can significantly improve and balance laying hen serum estrogen level; Effective promotion laying hen fallopian tubal and ovary healthy development, accelerate follicle maturity; Promote the absorption to calcium, reduce calcium loss; Powerful abatement free radical, improves immunity of organisms; Reduce residues of pesticides, heavy metal ion and the worm's ovum etc. introduced because of raw material simultaneously, substantially increase the foodsafety of feed.
2. ultrasonic extraction, high-pressure pulse electric extract and biological enzymolysis combination of sciences by modified dietary fiber of the present invention, gained modified dietary fiber Soluble Fiber cellulose content is high, more easily utilized by probio, improve probio in the growth of laying hen enteron aisle and fertility, increase kind and the quantity of probio flora, reduce laying hen gut pH, improve laying hen intestine microenvironment; High adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress laying hen to their absorption; Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger, effectively prevent laying hen heavy metal poisoning; The stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property.Regulate and maintain the resident time of gut flora, strengthen the absorption and digestion ability of enteron aisle, improve immunity of organisms; Effective promotion gastrointestinal peristalsis, slows down and eliminates the bad reaction such as gasteremphraxis, abdominal distension; Powerful bury function can prevent environment (oxygen, temperature, illumination, water activity etc.) factor from, on the impact of product quality, stabilizing the biologically active of feed, extending the shelf-life of feed.
3. Chinese herbal medicine extract science of the present invention is composite significantly improves laying hen and resists hot and cold irritability, disease resistance and immunity; Effective increase laying hen fallopian tubal and ovary weight, accelerate follicle maturity; The Chinese herbal medicine accelerating nutrients deposition is raw material, prepare through ultrasonic cleaning, low temperature enzymolysis, water extraction alcohol extracting and ultrasonic assistant Microwave Extraction, extract active constituent content is high, and cost is low, effectively can prevent the generation of salpingitis and oaritis, improve the reproductive performance of laying hen comprehensively.
4. bee pollen of maize extract of the present invention is with bee pollen of maize, corn stigma for raw material, successively through ultrasonic cleaning, high-pressure pulse electric and ultrasonic process, biological enzymolysis and obtained; Achieve: 1) ultrasonic cleaning effectively can remove the residues of pesticides of bee pollen of maize, waxy corn palpus, improves the foodsafety of feed; 2) high-pressure pulse electric process, has effectively killed the worm's ovum in bee pollen of maize and waxy corn palpus and miscellaneous bacteria, for postorder enzymolysis process normally carry out provide good gnotobasis; 3) omnidistance broken wall, extraction (high-pressure pulse electric, ultrasonic, enzymolysis), improve material effective component recovery rate and nutrient composition content; 4) macro-nutrients (protein, starch, fat etc.) both can be converted into Small molecular and easily absorb nutriment by biological enzymolysis, can realize again the broken wall to bee pollen of maize further; 5) omnidistance employing low temperature extractive technique, effectively prevent the loss of reproducibility active ingredient and volatile fragrant components (in waxy corn palpus), through water extraction and alcohol extracting, extract contains the bee pollen of maize of high level and the active ingredient of waxy corn palpus, effectively can promote that laying hen is to the absorption of nutriment, improve laying hen overall immunity, also has strong natural corn fragrance simultaneously, significantly improve the corn fragrance of material effective component content and finished product, enhance laying hen appetite, improve the uptake ratio of nutritional labeling, digestibility and absorptivity.
5. the present invention promotes that the feed producing method technique of laying hen ovary and Oviduct Development is simple, easy to operate, low for equipment requirements, is produced on a large scale.It should be noted that the feeding effect of this feed is each component collaborative, interactional result mutually, the not superposition of simple raw material function, the science of each raw material components is composite and extract, the effect produced, considerably beyond the superposition of each single component function and effect, has advanced and practicality preferably.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of plant extracts:
The preparation method of described plant extracts, comprise the following steps: northeast soybean and red clover are put into respectively the supersonic wave cleaning machine that 0.4% sodium bicarbonate solution is housed and clean 8min in 200W, 40KHz, drain, in mass ratio 7:5 Homogeneous phase mixing, then in-20 DEG C of freezing 8min; Be crushed to particle diameter 1mm immediately; To put in container and to add the benzinum of 12 times of weight, putting into water bath chader 40 DEG C concussion 2h, suction filtration, drying to obtain mixed powder; Adding 60% ethanol of mixed powder quality 20 times, is 4.5 by lactic acid adjust ph, and at electric-field intensity 30kV/cm under room temperature, burst length 400 μ s, carries out high-pressure pulse electric process under pulse frequency 250Hz condition; Then in 35 DEG C of insulation 1.5h, be then warming up to 45 DEG C and under power 200W condition, carry out the extraction of intermittent type microwave irradiation 18min, wherein microwave irradiation 10s, interval 20s; Under power 300W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Extracting liquid filtering, obtains filtrate, filter residue 2 times of weight, 52 DEG C of water rinses 3 times, and rinsing liquid and filtrate merge, Homogeneous phase mixing, and being evaporated to solid content is more than 15%, and namely freeze drying obtains plant extracts;
In the plant extracts filtrate adopting said method to prepare, the extracted amount of isoflavones is 24.13mg/g, and crude extract yield is 36%, and crude extract purity is 3.82%.
2. the preparation of modified dietary fiber:
The preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 6:5:4 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 300W, 40KHz condition ultrasonic extraction 12min, then at electric-field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 300Hz condition; Be 5.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains modified dietary fiber;
Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 8:4:3:3:2 Homogeneous phase mixing in mass ratio.
3. the preparation of Chinese herbal medicine extract:
The preparation method of described Chinese herbal medicine extract, comprises the steps: to count by weight, takes the Radix Astragali 18 parts, motherwort 17 parts, barrenwort 15 parts, hawthorn 14 parts, dried orange peel 13 parts, 12 parts, Radix Glycyrrhizae, rhizoma zingiberis 12 parts, Ligusticum wallichii 10 parts, the tuber of multiflower knotweed 8 parts, Radix Angelicae Sinensis 8 parts, Radix Codonopsis 8 parts, the bark of eucommia 7 parts, the seed of Chinese dodder 5 parts, the fruit of glossy privet 5 parts, oyster 4 parts, giant knotweed 4 parts; Put in the supersonic wave cleaning machine filling 0.2% sodium bicarbonate solution and clean 12min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is less than 2 millimeters, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtaining mixed material, control temperature 80 DEG C keeps 3h, then being cooled to 45 DEG C, is 4 by lactic acid adjust ph, under power 200W condition, carry out Microwave Extraction 12min, under power 250W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the mixed enzyme adding mixed material gross weight 1.0% carries out enzymolysis, be 6.2 by lactic acid adjust ph, enzymolysis 3h, finally add the mixture of mixed material 2 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 3.5h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 3:2, filter vacuum concentrates postlyophilization, pulverizes and obtains Chinese herbal medicine extract;
Described mixed enzyme is dextranase, zytase, pentosanase, pectase 3:3:2:1.5 Homogeneous phase mixing in mass ratio.
4. the preparation of bee pollen of maize extract:
The preparation method of described bee pollen of maize extract, comprise the steps: that 30:4 takes bee pollen of maize and corn stigma respectively in mass ratio, put in the supersonic wave cleaning machine that 0.4% sodium bicarbonate solution is housed, 4min is cleaned in 200W, 40KHz under room temperature, drain, put into container, add the water of former bee pollen of maize quality 4 times, be 5 by lactic acid adjust ph, in electric-field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric process under pulse frequency 300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 12min; Be warming up to 45 DEG C, add the compound enzyme of former bee pollen of maize quality 4%, stir, enzymolysis 18min, enzymolysis liquid liquid filters through 200 eye mesh screens, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, reduced pressure concentration, freeze drying;
The quality group of described compound enzyme becomes: protease: pectase: cellulase: lipase=12:6:5:2.
The plant extracts that following embodiment 2-6 uses, modified dietary fiber, Chinese herbal medicine extract, bee pollen of maize extract are embodiment 1 to be prepared;
Compound premix is that Liaoning Hefeng Animal Husbandry Co., Ltd produces, and pre-word (2011) 003024 is raised by product authentication code--the Liao Dynasty, and product consists of B B-complex and composite trace element, and trade mark is that standing grain is rich;
Complex enzyme be Dongguan Pan Asia too Bioisystech Co., Ltd produce PK6005, its trade mark is Bao Laikang Pokazyme, and main component is zytase 3000U/g, mannase: 800U/g, acid protease 3000U/g, lipase 800U/g, amylase 300U/g, dextranase 400U/g.
Probiotic is that Shandong Baolai-leelai Bio-engineering Co., Ltd. produces, and main component is that high enzyme is lived the bacillus subtilis of characteristic, lactobacillus acidophilus, saccharomyces cerevisiae, filamentous fungi and metabolite protease, amylase, NSP enzyme etc.Registration mark is that Bora profit is come;
Choline Chloride: Zhong Xin bio tech ltd, Liaoning, main component choline, registration mark is sky wing;
Phytase: Beijing Smistyle Science & Technology Development Co., Ltd., main component phytase.Registration mark is sunrise ocean;
Organic Chromium: chromium comes U.S. 990, and a new Chemical Co., Ltd. of U.S. of Mianyang, Sichuan city, active ingredient is chromium picolinate.
Embodiment 2
A kind of feed promoting laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight: corn 53 parts, dregs of beans 13 parts, modified dietary fiber 14 parts, stone flour 8 parts, calcium monohydrogen phosphate 0.8 part, fermented bean dregs 3 parts, DDGS 3 parts, plant extracts 4 parts, Chinese herbal medicine extract 4, cotton dregs 2 parts, rice bran meal 5 parts, 5 parts, rice bran, the prebiotic factor 3 parts, rapeseed dregs 2 parts, bee pollen of maize extract 5 parts, vegetable oil 1.7 parts, amino acid/11 .5 part, compound premix 0.5 part, complex enzyme 0.07 part, salt 0.3 part, Choline Chloride 0.08 part, probiotic 0.07 part, zinc methionine 0.04 part, phytase 0.02 part, Organic Chromium 0.002 part, Organic Selenium 0.002 part,
Described prebiotic factor percentage by weight consists of oligoisomaltose 35%, FOS 25%, stachyose 25%, oligomeric maltose 15%;
Described amino acid is lysine, methionine, threonine and tryptophan 1:1:0.5:0.2 Homogeneous phase mixing in mass ratio;
The preparation method of the feed of described promotion laying hen ovary and Oviduct Development, comprise the following steps: accurately take each component raw material according to formula, all pulverize according to laying hen material Conventional processing methods, then arrogantly by weight surplus stock is added successively to little, Homogeneous phase mixing, processing, must promote the feed of laying hen ovary and Oviduct Development.
Embodiment 3
A kind of feed promoting laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight: corn 40 parts, wheat 15 parts, dregs of beans 10 parts, modified dietary fiber 13 parts, stone flour 9 parts, calcium monohydrogen phosphate 0.8 part, fermented bean dregs 5 parts, corn germ cake 2 parts, plant extracts 2 parts, Chinese herbal medicine extract 2 parts, cotton dregs 2 parts, rice bran meal 1 part, 1 part, rice bran, oligoisomaltose 2 parts, bee pollen of maize extract 4 parts, vegetable oil 1.0 parts, lysine 0.2 part, methionine 0.15 part, compound premix 0.5 part, complex enzyme 0.05 part, salt 0.2 part, Choline Chloride 0.1 part, probiotic 0.05 part, zinc methionine 0.01 part, phytase 0.01 part, Organic Chromium 0.001 part, Organic Selenium 0.001 part,
The preparation method of the feed of described promotion laying hen ovary and Oviduct Development is with embodiment 2.
Embodiment 4
A kind of feed promoting laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight: corn 55 parts, dregs of beans 10 parts, modified dietary fiber 15 parts, stone flour 8.5 parts, calcium monohydrogen phosphate 0.8 part, fermented bean dregs 5 parts, corn bran 3 parts, plant extracts 5 parts, Chinese herbal medicine extract 5 parts, cotton dregs 2 parts, rice bran meal 8 parts, 5 parts, rice bran, FOS 4 parts, rapeseed dregs 3 parts, bee pollen of maize extract 6 parts, vegetable oil 3 parts, lysine 1 part, methionine 0.9 part, threonine 0.8 part, tryptophan 0.3 part, compound premix 0.5 part, complex enzyme 0.1 part, salt 0.4 part, Choline Chloride 0.12 part, probiotic 0.1 part, zinc methionine 0.07 part, phytase 0.03 part, Organic Chromium 0.003 part, Organic Selenium 0.003 part,
The preparation method of the feed of described promotion laying hen ovary and Oviduct Development is with embodiment 2.
Embodiment 5
A kind of feed promoting laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight: corn 45 parts, Chinese sorghum 10 parts, dregs of beans 20 parts, modified dietary fiber 13 parts, stone flour 9 parts, calcium monohydrogen phosphate 0.8 part, fermented bean dregs 5 parts, DDGS3 part, corn germ cake 3 parts, plant extracts 2 parts, Chinese herbal medicine extract 5 parts, cotton dregs 2 parts, rice bran meal 3 parts, 1 part, rice bran, the prebiotic factor 4 parts, bee pollen of maize extract 6 parts, vegetable oil 1 part, amino acid/11 part, compound premix 0.5 part, complex enzyme 0.05 part, salt 0.3 part, Choline Chloride 0.12 part, probiotic 0.05 part, zinc methionine 0.07 part, phytase 0.01 part, Organic Chromium 0.003 part, Organic Selenium 0.001 part,
Described prebiotic factor percentage by weight consists of oligoisomaltose 30%, FOS 30%, stachyose 20%, oligomeric maltose 20%;
Described amino acid is lysine, methionine, threonine and tryptophan 1:1:0.5:0.2 Homogeneous phase mixing in mass ratio;
The preparation method of the feed of described promotion laying hen ovary and Oviduct Development is with embodiment 2.
Embodiment 6
A kind of feed promoting laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight: corn 40 parts, 20 parts, rice, dregs of beans 5 parts, modified dietary fiber 15 parts, stone flour 9 parts, calcium monohydrogen phosphate 0.8 part, fermented bean dregs 8 parts, DDGS2 part, plant extracts 5 parts, Chinese herbal medicine extract 2 parts, cotton dregs 2 parts, rice bran meal 1 part, 8 parts, rice bran, the prebiotic factor 2 parts, rapeseed dregs 3 parts, bee pollen of maize extract 4 parts, vegetable oil 1 part, 0.4 part, amino acid, compound premix 0.5 part, complex enzyme 0.07 part, salt 0.3 part, Choline Chloride 0.05 part, probiotic 0.1 part, zinc methionine 0.01 part, phytase 0.03 part, Organic Chromium 0.001 part, Organic Selenium 0.003 part,
Described prebiotic factor percentage by weight consists of oligoisomaltose 40%, FOS 20%, stachyose 20%, oligomeric maltose 20%;
Described amino acid is lysine, methionine, threonine and tryptophan 1:1.2:0.5:0.2 Homogeneous phase mixing in mass ratio;
The preparation method of the feed of described promotion laying hen ovary and Oviduct Development is with embodiment 2.
Feed of the present invention in 5% laying rate-32 week age grade section on the impact of laying hen reproductive performance and production performance
1. in test period: tested on September 10th, 2014---on January 15th, 2015, amount to 125 days, wherein 7 days preliminary trial periods, 105 days experimental periods, test prepares and terminates 3 days.
2. test site: test and implement at Huan Ren Xinhua chicken house field experiment.
3. test group design: test group is divided into control group and feed group of the present invention; Wherein control group is that market material 1 is laid eggs peak material, and testing material 2 is laid eggs peak material, and test group is promotion laying hen ovary and the feed of Oviduct Development prepared by the embodiment of the present invention 2 of feeding.Test adopts single factor experiment design, divides 10 times, and often criticize the blue brown 120 age in days laying hens in selection 1440 sea, be divided into 3 groups, often group establishes 5 repetitions, each repetition 96 chickens.
4. feeding method: manual material loading, every day 3 times, free choice feeding; Freely drink water, water is underground water, water temperature 15-21 DEG C.
5. hen house temperature: adopt longitudinal ventilation, hen house temperature controls at 20-28 DEG C.Strictly control according to the blue brown feeding and management in sea.
6. testing inspection index: 1) start body weight and terminate body weight, shin length, feed intake, survival rate.2) to lay eggs a piece number, egg size, broken egg and soft-preserved egg; 3) slaughter experiment: fallopian tubal length and weight, ovary weight, rheum officinale ovarian follicle number, little ovarian follicle quantity.
7. result of the test: by every batch experiment Indexs measure result statistics, calculate 10 batches of averages, statistics is as table 1,2 and 3:
Table 1: on the impact of laying hen organ of multiplication in 32 week age
Note: data alphabet of going together is completely different represents significant difference (P < 0.05), and alphabetical identical table differential is different not significantly (P > 0.05), and following table is same.
From upper table, compared with control group (market group 1,2), of the present invention group has remarkable facilitation to fallopian tubal during 32 week age of laying hen and the development of ovary, fallopian tubal length is long 6.82cm and 4.62cm respectively, fallopian tubal weight fraction you can well imagine high 11.49g and 7.46g, ovary weight improves 6.08g and 4.17g respectively, significant difference.The quantity of large yellow ovarian follicle many 2.2 and 1.8 respectively, little ovarian follicle quantity many 3.6 and 3.1 respectively.As can be seen here, of the present invention group has significant facilitation to laying hen upper peak fallopian tubal in early stage and the development of ovary.
Laying hen reproductive system comprises ovary and fallopian tubal, and ovary comprises base portion and a large amount of ovarian follicle, and follicular development is closely related with egg laying performance, and when large follicle quantity is many, continuous egg laying performance is strong, and laying rate is high.
Table 2: on the impact of Layer Production Performance
| Of the present invention group | Market material 1 | Market material 2 | |
| Feed intake (gram) | 110.30±0.29 | 109.98±0.12 | 110.50±0.10 |
| Laying rate (%) | 95.60±1.33 a | 93.53±1.99 b | 93.45±1.13 b |
| Egg size (gram) | 63.06±0.13 a | 60.33±0.44 b | 61.14±0.47 b |
| Feedstuff-egg ratio | 1.92±0.02 | 2.07±0.02 | 1.97±0.02 |
| Egg production (gram/only/day) | 58.16±0.91 a | 55.43±1.51 b | 56.24±1.09 b |
The organ of multiplication such as ovary, fallopian tubal is grown, and show that laying rate aspect is just obvious, as seen from the above table, compared with control group (market group 1,2), of the present invention group of laying rate improves 2.07% and 2.15% respectively.Egg production improves 2.73 grams and 1.92 grams respectively, has good economic benefit.
Table 3: on the impact of laying hen body weight and tibia length
| Of the present invention group | Market material 1 | Market material 2 | |
| Body weight (kilogram) | 1.95 | 1.88 | 1.90 |
| Shin long (millimeter) | 111 | 104 | 106 |
| Survival rate (%) | 99.85 | 97.45 | 96.65 |
Use of the present invention group of feed, body weight control is in SBW scope, high 40 grams compared with standard-required, is conducive to the performance of body health and egg laying performance.Compared with control group (market group 1,2), of the present invention group of long long 7mm and 5mm respectively of shin, body weight improves 0.07kg and 0.05kg respectively, and body weight and build are obviously better than market group, and field observation of the present invention group of build is delicate and pretty compact, solid.Survival rate improves 2.4% and 3.2% respectively, demonstrates the stronger anti-immunity of laying hen and disease resistance.
It should be noted that, feed prepared by embodiment of the present invention 3-6 has the feeding effect of above-mentioned table 1-3 equally, and between each embodiment, Indexs measure result difference is not remarkable, has good reproductive ability, productivity, anti-immune and disease resistance.
Claims (10)
1. one kind promotes the feed of laying hen ovary and Oviduct Development, prepared by the raw material of following parts by weight: cereal 53-60 part, dregs of beans 5-20 part, modified dietary fiber 13-15 part, fermented bean dregs 3-8 part, corn byproducts 2-6 part, plant extracts 2-5 part, Chinese herbal medicine extract 2-5 part, cotton dregs 2 parts, rice bran meal 1-8 part, rice bran 1-8 part, stone flour 8-9 part, calcium monohydrogen phosphate 0.8 part, prebiotic factor 2-4 part, rapeseed dregs 0-3 part, bee pollen of maize extract 4-6 part, vegetable oil 1-3 part, amino acid 0.35-3 part, compound premix 0.5 part, complex enzyme 0.05-0.1 part, salt 0.2 ~ 0.4 part, Choline Chloride 0.05-0.12 part, probiotic 0.05-0.1 part, zinc methionine 0.01-0.07 part, phytase 0.01-0.03 part, Organic Chromium 0.001 ~ 0.003 part, Organic Selenium 0.001-0.003 part,
To be corn or corn mix with any one in rice, wheat, Chinese sorghum described cereal;
Described corn byproducts is one or more mixing in DDGS, corn germ cake, corn bran;
The described prebiotic factor is at least one in oligoisomaltose, FOS, stachyose, oligomeric maltose;
Described plant extracts be soybean, clover are adopted ultrasonic cleaning, freezing, pulverize, high-pressure pulse electric extracts, ultrasonic wave added Microwave Extraction technology and obtaining.
2. the feed promoting laying hen ovary and Oviduct Development as claimed in claim 1, it is characterized in that, the preparation method of described plant extracts, comprise the following steps: northeast soybean and red clover are put into respectively the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed and clean 5-10min in 200W, 40KHz, drain, 5-9:3-6 Homogeneous phase mixing in mass ratio, then in-18--23 DEG C of freezing 5-10min; Be crushed to particle diameter 0.5-1.5mm immediately; To put in container and to add the benzinum of 10-15 times of weight, putting into water bath chader 35-45 DEG C of concussion 1-3h, suction filtration, drying to obtain mixed powder; Add mixed powder quality 15-25 60% ethanol doubly, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, carries out high-pressure pulse electric process under pulse frequency 200-300Hz condition; Then in 30-40 DEG C of insulation 1-2h, be then warming up to 40-50 DEG C under power 150-300W condition, carry out microwave irradiation extraction 15-20min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Extracting liquid filtering, obtains filtrate, and filter residue 1-3 times of weight 45-60 DEG C of water rinse 3 times, rinsing liquid and filtrate merge, Homogeneous phase mixing, and being evaporated to solid content is more than 15%, and namely freeze drying obtains plant extracts.
3. the feed promoting laying hen ovary and Oviduct Development as claimed in claim 2, is characterized in that, described microwave irradiation is extracted as batch (-type) and extracts, i.e. microwave irradiation 10s, interval 20s.
4. the feed promoting laying hen ovary and Oviduct Development as claimed in claim 1, it is characterized in that, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 5-7:4-6:3-5 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;
Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 7-9:3-5:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.
5. the feed promoting laying hen ovary and Oviduct Development as claimed in claim 1, it is characterized in that, the preparation method of described Chinese herbal medicine extract, comprise the steps: to count by weight, take Radix Astragali 15-20 part, motherwort 15-20 part, barrenwort 13-18 part, hawthorn 12-15 part, dried orange peel 12-15 part, Radix Glycyrrhizae 10-15 part, rhizoma zingiberis 10-15 part, Ligusticum wallichii 8-12 part, tuber of multiflower knotweed 6-10 part, Radix Angelicae Sinensis 6-10 part, Radix Codonopsis 6-10 part, bark of eucommia 5-8 part, seed of Chinese dodder 3-7 part, fruit of glossy privet 3-7 part, oyster 3-5 part, giant knotweed 2-5 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is less than 2 millimeters, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70 DEG C-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, Microwave Extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the mixed enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter, obtain the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85 DEG C-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, filter vacuum concentrates postlyophilization, pulverizes and obtains Chinese herbal medicine extract;
Described mixed enzyme is dextranase, zytase, pentosanase, pectase 2-4:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.
6. the feed promoting laying hen ovary and Oviduct Development as claimed in claim 1, it is characterized in that, the preparation method of described bee pollen of maize extract, comprise the steps: that 20-40:3-5 takes bee pollen of maize and corn stigma respectively in mass ratio, put in the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed, in 200W under room temperature, 40KHz cleans 3-5min, drain, put into container, add former bee pollen of maize quality 3-5 water doubly, be 4-6 by lactic acid adjust ph, in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 10-15min, be warming up to 40-50 DEG C, add the compound enzyme of former bee pollen of maize quality 3-5%, stir, enzymolysis 15-20min, enzymolysis liquid liquid filters through 100-300 eye mesh screen, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, reduced pressure concentration, freeze drying,
The quality group of described compound enzyme becomes: protease: pectase: cellulase: lipase=11-13:5-7:4-6:1-3.
7. the feed promoting laying hen ovary and Oviduct Development as claimed in claim 1, it is characterized in that, described prebiotic factor percentage by weight consists of oligoisomaltose 30-40%, FOS 20-30%, stachyose 20-30%, oligomeric maltose 15-25%.
8. the preparation method promoting the feed of laying hen ovary and Oviduct Development as claimed in claim 1, it is characterized in that, comprise the following steps: accurately take each component raw material according to formula, all conveniently laying hen material processing method is pulverized, then arrogantly by weight surplus stock is added successively to little, Homogeneous phase mixing, processing, must promote the feed of laying hen ovary and Oviduct Development.
9. promote the preparation method of the feed of laying hen ovary and Oviduct Development as claimed in claim 8, it is characterized in that, described amino acid is the mixing of in lysine, methionine, threonine or tryptophan two kinds or more.
10. promote the preparation method of the feed of laying hen ovary and Oviduct Development as claimed in claim 9, it is characterized in that, described amino acid is lysine, methionine, threonine and tryptophan 1:0.8-1.2:0.5-1.0:0.2-0.5 Homogeneous phase mixing in mass ratio.
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