CN111955621B - Application of composite polypeptide in preparation of feed for promoting egg laying of chickens - Google Patents
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Abstract
The invention provides application of a composite polypeptide in preparation of a feed for promoting egg laying of chickens, and belongs to the technical field of feed preparation. The raw materials for preparing the composite polypeptide provided by the invention are mussel, corbicula fluminea and razor clam. Wherein, according to the mass ratio, the mussel: white clam: the razor clam is 5. The composite polypeptide prepared by the invention can effectively promote the proliferation of the chicken ovarian granulosa cells and can effectively inhibit the apoptosis of the chicken ovarian granulosa cells. Therefore, the composite polypeptide prepared by the invention can be used for preparing feed for promoting egg laying of chickens.
Description
Technical Field
The invention belongs to the technical field of livestock feed, and particularly relates to application of a compound polypeptide in preparation of feed for promoting egg laying of chickens.
Background
The main products of the chicken industry are eggs and chicken. Among them, the egg is a low-cost and high-efficiency animal protein source, the egg contains rich nutrient substances, and the proportion of amino acids in the egg is suitable for the requirements of human bodies, so the egg is well received by consumers. Meanwhile, eggs are also important carriers for breeding the next generation of chickens. Therefore, in modern commercial production, the egg laying performance of eggs has a significant influence on both broiler and laying hens. Studies have shown that the egg laying performance of poultry is largely determined by the developmental status of the follicles.
Granulosa cells are important cells that make up follicles and are derived from endodermal epithelial cells. During the mature period of follicular development, the differentiation and growth of granulosa cells are closely related to the developmental process of follicles. Granulosa cells are rapidly brought into a differentiated state from undifferentiated cells before and after follicle selection, and the granulosa cells can regulate the development of follicles through synthesis of hormones and growth factors, so that the granulosa cells play an important role in regulating the recruitment of follicles, the selection of follicles, and the growth, development and maturation of follicles. Therefore, the effective promotion of the growth of the granulosa cells can effectively promote the growth of chicken follicles and the laying rate of chickens.
Disclosure of Invention
The invention aims to provide application of a composite polypeptide in preparation of an egg laying feed.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides an application of a composite polypeptide in preparing feed for promoting egg laying of chickens, wherein the composite polypeptide is prepared from the following raw materials: mussels, corbicula alba and razor clams.
Preferably, in the raw materials for preparation, the weight ratio of the mussels: white clam: the razor clam is 5.
Preferably, the preparation method of the composite polypeptide comprises the following steps:
(1) Cleaning mussel, corbicula fluminea and razor clam, drying, adding into a grinder according to the mass ratio of 5;
(2) Adjusting the pH to 5-6 in the composite homogenate at 50-60 ℃, adding 0.1-0.2 times of papain, and performing enzymolysis for 5-6 hours;
(3) Adjusting the temperature to 95-98 deg.C, inactivating enzyme for 15-25min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) And (4) carrying out vacuum freeze drying to obtain the composite polypeptide.
In addition, the invention provides an application of the composite polypeptide in preparing the accelerant for promoting the proliferation of the chicken ovarian granulosa cells, and the preparation method of the composite polypeptide comprises the following steps:
(1) Cleaning mussel, corbicula fluminea and razor clam, drying, adding into a grinder according to the mass ratio of 5;
(2) Adjusting the pH to 5-6 and the temperature to 50-60 ℃ in the composite homogenate, adding 0.1-0.2 times of papain, and carrying out enzymolysis for 5-6 hours;
(3) Adjusting the temperature to 95-98 deg.C, inactivating enzyme for 15-25min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) And (4) carrying out vacuum freeze drying to obtain the composite polypeptide.
In addition, the invention provides an application of the composite polypeptide in preparing an inhibitor for inhibiting apoptosis of chicken ovarian granular cells, and the preparation method of the composite polypeptide comprises the following steps:
(1) Cleaning mussel, corbicula fluminea and razor clam, drying, adding into a grinder according to the mass ratio of 5;
(2) Adjusting the pH to 5-6 and the temperature to 50-60 ℃ in the composite homogenate, adding 0.1-0.2 times of papain, and carrying out enzymolysis for 5-6 hours;
(3) Adjusting the temperature to 95-98 deg.C, inactivating enzyme for 15-25min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) And (4) carrying out vacuum freeze drying to obtain the composite polypeptide.
In addition, the invention provides a composite polypeptide, which is prepared from mussel, corbicula fluminea and razor clam as raw materials;
according to the mass ratio, mussels: white clam: the razor clam is 5;
the preparation method of the composite polypeptide comprises the following steps:
(1) Cleaning mussel, corbicula fluminea and razor clam, drying, adding into a grinder according to the mass ratio of 5;
(2) Adjusting the pH to 5-6 in the composite homogenate at 50-60 ℃, adding 0.1-0.2 times of papain, and performing enzymolysis for 5-6 hours;
(3) Adjusting the temperature to 95-98 deg.C, inactivating enzyme for 15-25min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) Vacuum freeze drying to obtain composite polypeptide;
the composite polypeptide is used for preparing feed for promoting chicken to lay eggs.
The invention has the beneficial effects that:
the prepared composite polypeptide can effectively promote the proliferation of chicken ovarian granular cells; inhibiting the apoptosis of chicken ovarian granulosa cells. As the granulosa cells can effectively promote the development of chicken follicles, the composite polypeptide prepared by the invention can be used for preparing feed for promoting the laying of chickens.
Drawings
FIG. 1 Effect of different concentrations of Complex polypeptide A on the proliferation of chicken ovarian granulosa cells.
FIG. 2 Effect of 100mg/ml Complex polypeptide A on chicken ovarian granulosa cells CDK-2 gene expression.
FIG. 3 shows the effect of 100mg/ml of composite polypeptide A on chicken ovarian granulosa cell Cyclin-E gene expression.
FIG. 4 is a graph showing the effect of 100mg/ml of composite polypeptide A on the apoptosis fluorescence intensity of chicken ovarian granulosa cells.
FIG. 5 shows that 100mg/ml of composite polypeptide A is used for treating chicken ovarian granulosa cell apoptosis-related protein Caspase
3 and Bcl-2.
Detailed Description
The present invention is further described with reference to the following examples, which are intended to be illustrative of the preferred embodiments of the invention only, and not to be limiting of the invention in any way. Any simple modification or equivalent changes made to the following embodiments according to the technical essence of the present invention, without departing from the technical spirit of the present invention, fall within the scope of the present invention.
Example 1
(1) Cleaning 500g of mussel, 200g of Corbicula fluminea and 300g of razor clam, drying, adding into a grinder, grinding into powder, adding 3000g of water, and making into composite homogenate;
(2) Adjusting the pH to 5 and the temperature to 50 ℃ in the composite homogenate, adding 0.1 time of papain, and performing enzymolysis for 6 hours;
(3) Adjusting the temperature to 95 ℃, inactivating enzyme for 25min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) And (4) carrying out vacuum freeze drying to obtain the compound polypeptide A.
Example 2
(1) Cleaning 500g of mussel, 200g of Corbicula fluminea and 300g of razor clam, drying, adding into a grinder, grinding into powder, adding 3000g of water, and making into composite homogenate;
(2) Adjusting the pH to 6 and the temperature to 60 ℃ in the composite homogenate, adding 0.1 time of papain, and performing enzymolysis for 6 hours;
(3) Adjusting the temperature to 98 ℃, inactivating the enzyme for 15min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) And (4) carrying out vacuum freeze drying to obtain the compound polypeptide B.
Example 3
(1) Cleaning 500g of mussel, 200g of Corbicula fluminea and 300g of razor clam, drying, adding into a grinder, grinding into powder, adding 3000g of water, and preparing into composite homogenate;
(2) Adjusting the pH to 5 and the temperature to 55 ℃ in the composite homogenate, adding 0.1 time of papain, and performing enzymolysis for 6 hours;
(3) Adjusting the temperature to 96 ℃, inactivating the enzyme for 15min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) And (5) carrying out vacuum freeze drying to obtain the compound polypeptide C.
Example 3
Detecting influence of composite polypeptide A on chicken ovarian granulosa cell proliferation
1. Preparation of Chicken ovarian granulosa cells
(1) Killing the hen, removing chicken feather on the belly of the hen, disinfecting, carefully taking out the follicle, placing the follicle in a sterile culture medium containing PBS buffer solution, removing bloodiness by using the PBS buffer solution added with double antibodies, and rinsing for 3 times;
(2) The follicle is placed in PBS buffer solution added with double antibody to be mixed, the outer membrane, connective tissue, vascular network and the like of the follicle are completely removed, and the membrane layer and the granular layer are separated according to the method of Gilbert et al 1977;
(3) The separated granular cell layer was washed clean with PBS added with double antibody and sheared to 1mm 2 Placing the tissue blocks with the sizes in a centrifuge tube;
(4) Adding 1mg/ml collagenase II, digesting at 37 deg.C for 5-10min until no obvious tissue mass exists in the centrifuge tube, and adding 5ml M199 culture medium pre-cooled at 4 deg.C to stop digestion;
(5) Filtering the cell suspension by using a 70 mu m cell filter screen into a new 50ml centrifuge tube, centrifuging at 1000rpm for 10min;
(6) Adding M199 culture medium again to resuspend the precipitate, and centrifuging at 1000rpm for 10min;
(7) Inoculating the cells into a culture dish, and putting the culture dish into a cell culture box for continuous culture to obtain the chicken ovarian granular cells.
2. Detecting influence of composite polypeptide on chicken ovarian granulosa cell proliferation
(1) Grouping cells: a control group, a 10mg/ml compound polypeptide group A, a 50mg/ml compound polypeptide group A, a 100mg/ml compound polypeptide group A and a 150mg/ml compound polypeptide group A;
(2) According to the groups, 100. Mu.l of 2X 10 in the logarithmic growth phase 4 Individual cells were seeded in 96-well plates, with 3 replicates per group;
(4) The cells were cultured in a cell incubator for 48 hours, and the absorbance was measured at 450nm using CCK-8.
The results are shown in FIG. 1, from which it can be seen that 10mg/ml of the composite polypeptide group A (0.931. + -. 0.033) had no significant effect on granulosa cell proliferation; 50mg/ml composite polypeptide group A (1.177 +/-0.027, P < -0.001) has small promoting effect on the proliferation of granulosa cells; the 100mg/ml composite polysaccharide group A (1.450 +/-0.068, P < -0.001) has a remarkable promoting effect on the proliferation of granular cells; 150mg/ml complex polysaccharide group A (1.485 + -0.034, P < -0.0001) also had significant enhancement of granulosa cell proliferation, but was not significantly upregulated relative to 100mg/ml, and therefore 100mg/ml complex polypeptide A was selected for subsequent experiments.
Example 4
Detecting the effect of the composite polypeptide on the chicken ovarian granulosa cell proliferation related gene
1. Cell grouping and processing
(1) Inoculating the chicken ovarian granulosa cells into a 6-well plate, after the cells are attached to the wall, adding 100mg/ml of the composite polypeptide A into the experimental group without treatment, and extracting RNA after 48 hours of treatment.
2.2. RNA extraction
(1) Removing the culture medium, adding 1ml of Triquick Reagent, blowing and beating the cells by using a liquid moving machine to fully crack the cells, standing at room temperature for 5min, and transferring the cells into a centrifuge tube;
(2) Adding 0.2ml of chloroform into a centrifuge tube, uniformly mixing, reacting for 5min at room temperature, and centrifuging for 10min at 12000g and 4 ℃;
(3) Absorbing the upper layer of water phase into a new centrifugal tube, adding 0.5ml of isopropanol, and reversing and uniformly mixing;
(4) Standing at room temperature for 10min, centrifuging at 12000g 4 deg.C for 10min;
(5) Adding 1ml 75% ethanol (DEPC water preparation) to dissolve precipitate, centrifuging at 12000g 4 deg.C for 5min, and removing supernatant;
(6) 50 μ L of DEPC was added to dissolve the RNA.
3. Reverse transcription reaction
(1) gDNA removal according to Tiangen reverse transcription kit
gDNA reaction system
| Composition of matter | Amount of the use |
| 5×gDNA Buffer | 2μL |
| TotaL RNA | 1μg |
| RNase-Free ddH2O | Make up to 10. Mu.L |
(2) Centrifuging with a small centrifuge, placing in a PCR instrument, incubating at 42 deg.C for 3min, and placing on ice;
(3) Configuring a reverse transcription reaction System
| Reagent | Amount of the use |
| FastKing RT Enzyme Mix | 2μL |
| Step 1 products | 1μL |
| 10×King RT Buffer | 2μL |
| FQ-RT Primer Mix | 2μL |
| RNase-Free ddH2O | 3μL |
(4) Placing the mixed solution in a common PCR instrument at 42 ℃ for 15min;95 ℃ for 3min.
4. Real-time fluorescent quantitative PCR
The primer sequences are as follows:
| gene | ID | Fluorescent quantitative primer sequence (5 '-3') | Size of product |
| β-actin | NM_205518 | AATGGCTCCGGTATGTGCAA | 112 |
| GGCCCATACCAACCATCACA | |||
| CDK2 | NM_001199857 | CTGTGGACATCTGGAGCCTG | 107 |
| CGTGCGGAAGATACGGAAGA | |||
| Cyclin-E | NM_001031358 | GAACACCGAAGAGTGCTCCA | 149 |
| AGTTGTCAACAGGGGACAGC |
The reaction system is as follows:
| reagent | Amount of the use |
| cDNA | 1μL |
| Upstream primer | 0.5μL |
| Downstream primer | 0.5μL |
| SYBR Mix | 5μL |
| ddH2O | 3μL |
A reaction system:
1min at 95 ℃;95 ℃ 15s,60 ℃ 30s,40 cycles.
The experimental results are shown in fig. 2 and 3:
the relative expression level of CDK2 is 1.446 +/-0.075, the relative expression level of Cyclin-E is 1.509 +/-0.045, and the differences have statistical significance, so that the composite polypeptide A prepared by the invention can effectively promote proliferation-related genes CDK2 and Cyclin-E.
Example 5
Detecting influence of composite polypeptide A on chicken ovarian granular cell apoptosis
Detection Using the Shanghai worker Caspase 3/7 Activity Apoptosis Assay Kit
(1) 2 x 10 of 4 Inoculating each ovarian granulosa cell in a 96-well plate, after overnight culture, not processing a control group, replacing an experimental group with a 100mg/ml compound polysaccharide A culture medium, and setting 3 repeats for each group;
(2) After 24h treatment, the cells were washed 3 times with PBS, 5min each time;
(3) Adding 50 mu L of Caspase 3/7 substrate (component A) into 10ml of Assay Buffer (component B) and uniformly mixing to prepare Caspase 3/7 detection Buffer solution;
(4) 100 mu L of Caspase 3/7 detection buffer is added into each detection well plate, the plates are incubated for 1h in the dark at room temperature after the buffer is added, the fluorescence intensity at 490/525nm is detected, the fluorescence intensity of a control group is set to be 1, and the result is shown in figure 4.
As can be seen from the figure, the relative fluorescence intensity of the 100mg/ml composite polypeptide A group is 0.642 +/-0.040, and the difference is statistically significant, so that the composite polypeptide A can obviously reduce the apoptosis of chicken ovarian granulosa cells.
Example 6
1. Experimental treatment
(1) Inoculating the chicken ovarian granular cells into a 6-well plate, after the chicken ovarian granular cells are attached to the wall, not processing a control group, and adding 100mg/ml of composite polypeptide A into an experimental group;
(2) And (5) putting the mixture into a cell culture box, culturing for 48 hours, extracting protein, and carrying out Western Blot detection.
2. Protein extraction
(1) Discarding the culture medium, adding 1ml PBS to wash the cells, adding 100 μ l, scraping the cells with a cell scraper, and transferring the cells to an EP tube with a pipette;
(2) Cracking on ice for 30min, placing in a centrifuge, centrifuging at 4 deg.C and 12000rpm/min for 15min;
(3) After the centrifugation is finished, carefully sucking the supernatant to obtain a protein sample;
(4) The protein concentration was measured using the BCA method, and the protein sample concentration was adjusted to 2. Mu.g/. Mu.l.
3. Protein electrophoresis
(1) Preparing 12% separation gel and 5% concentrated gel, and adding 10 mul of protein sample and protein Marker into each hole;
(2) The constant pressure of the concentration gel is 90V, the constant pressure of the separation gel is 120V, and electrophoresis is stopped when bromophenol blue runs to the bottom of the gel;
(3) Carefully taking out the gel, installing a transfer printing clamp according to the modes of the spongy cushion, the three layers of filter paper, the PAGE glue, the PVDF film, the three layers of filter paper and the spongy cushion, putting the transfer printing clamp into an electric rotating groove, and electrically rotating for 1.5 hours at room temperature;
(4) After the electrotransformation is finished, the PVDF membrane is taken out, put into 5% skimmed milk powder and sealed for 1h by a shaking table at room temperature.
(5) After the sealing is finished, washing the membrane for 3 times by using TBST, and each time for 10 minutes;
(6) Cutting a PVDF membrane according to the size of a corresponding protein band, incubating Caspase3, bcl-2 and beta-actin primary antibodies, and incubating overnight at 4 ℃;
(7) After the primary antibody incubation is finished, washing the membrane for 3 times by using TBST, incubating corresponding 2 antibodies for 10 minutes each time, and incubating for 1 hour at room temperature;
(8) After the secondary antibody incubation was completed, the membrane was washed 3 times with TBST and development exposure was performed.
The results are shown in FIG. 5, from which it can be seen that composite polypeptide A can effectively reduce the activity of the proapoptotic protein Caspase3 and promote the expression of the antiapoptotic protein Bcl-2.
In conclusion, the composite polypeptide prepared by the invention can effectively promote the proliferation of the chicken ovarian granulosa cells and can effectively inhibit the apoptosis of the chicken ovarian granulosa cells. Therefore, the compound polysaccharide prepared by the invention can be used for preparing feed for promoting chicken to lay eggs.
Claims (1)
1. The application of the composite polypeptide in preparing the accelerant for promoting the proliferation of the ovarian granulosa cells of the chickens is characterized in that the preparation method of the composite polypeptide comprises the following steps:
(1) Cleaning mussel, corbicula fluminea and razor clam, drying, adding into a grinder according to the mass ratio of 5;
(2) Adjusting the pH to 5-6 in the composite homogenate at 50-60 ℃, adding 0.1-0.2 times of papain, and performing enzymolysis for 5-6 hours;
(3) Adjusting the temperature to 95-98 deg.C, inactivating enzyme for 15-25min, and cooling at room temperature;
(4) Centrifuging, collecting supernatant, and filtering with 0.25 μm ultrafiltration membrane to obtain composite polypeptide solution;
(5) Vacuum freeze drying to obtain composite polypeptide;
the concentration of the composite polypeptide in the accelerant is 50-150mg/mL.
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| CN115843929A (en) | 2023-03-28 |
| CN115843929B (en) | 2024-08-02 |
| CN111955621A (en) | 2020-11-20 |
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