CN1022538C - Method for preparing feed with aqueous soluble protein - Google Patents
Method for preparing feed with aqueous soluble protein Download PDFInfo
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- CN1022538C CN1022538C CN89105308A CN89105308A CN1022538C CN 1022538 C CN1022538 C CN 1022538C CN 89105308 A CN89105308 A CN 89105308A CN 89105308 A CN89105308 A CN 89105308A CN 1022538 C CN1022538 C CN 1022538C
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- soluble protein
- feed
- scallops
- aqueous soluble
- soft body
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to a method using scallops to process waste enzymolysis to manufacture water-soluble protein feed. The method is characterized in that additional proteolytic enzyme is used for changing the organization structures and the protein structures of the soft parts of scallops; the proportion of amino acid is adjusted by adding auxiliary materials to make the scallops adapt to the requirements of animal organisms; various ingredient in the soft parts of the scallops are efficiently used. The water-soluble protein feed manufactured by the method has the advantages of powdery shape, easy animal digestive absorption and convenient storage and transportation, and the water-soluble protein feed can replace import fish meal to feed chickens, pigs, martens, fishes, shrimps, etc.
Description
The invention relates to the method for making feed with aqueous soluble protein with the scallop processing waste enzymolysis.Say it promptly is to utilize to add protease and will be difficult for the scallop soft body that animal digestion absorbs and decompose in detail,, make it to become the manufacture method of powdery feed with aqueous soluble protein again through auxiliary material adsorption dry and adjust the amino acid ligand ratio.
Scallop is a kind of precious marine product of nutritious, unique flavor.Long ago, the coastal fisherman of China just knows and utilizes its closed shell flesh to make senior precious marine product-dried scallop, but the remaining software part in processing back is not fully utilized, a part of human consumption, and a part is used as the direct nutrition purposes, especially for feeding animals of feed, and major part is wasted.Though the scallop software partly is a kind of high-protein nutrient source, because its outer rim tentacle partly is to be made of hard ligament, so be difficult for being digested and assimilated by animal.Such as the fishes and shrimps etc. of feeding with the bright product of scallop soft body, the visceral mass that discovery only accounts for soft body 50% partly is ingested: with the scallop body dry powder small white mouse etc. of feeding, its absorptivity also is starkly lower than other similar product (seeing accompanying drawing).Ton surplus present domestic every year, fully the scallop soft body of processing and utilization did not reach 10,000, so many high-quality proteins are wasted, and are very unfortunate.
Purpose of the present invention just is to provide a kind of and can makes full use of high protein and the various physiology that the scallop soft body contained and regulate material, growth promoting substance, by adding protease hydrolytic, destroy its institutional framework, and adjust amino acid ligand through adsorption dry, auxiliary material and reasonable process such as compare, make powdery, be easy to the animal digestion absorption, be convenient to preserve the feed with aqueous soluble protein manufacture method of transportation.This feed with aqueous soluble protein can replace high-quality fish meal to feed chicken, pig, ermine, fish, shrimp etc.
In order to achieve the above object, manufacture method of the present invention has adopted following technology and process conditions.
One, technological process
New fresh scallops soft body → rubbing → preservative treatment → go into pond storage → neutralization → temperature control enzymolysis → auxiliary material absorption → Exposure to Sunlight → dry sterilization → pulverizing packing → product inspection → finished product
Two, technical process and condition stub
Select new fresh scallops soft body for use, rub, add 36N food grade concentrated hydrochloric acid, regulate acidity, stir, deposit in the cement pit that posts fiberglass standby to pH2.5 with meat grinder or pulverizer.If in the harvest season, can directly process without preservative treatment.Get by the scallop soft body of above method acid storage with CaO or Ca(OH) regulate pH value to 7, add in the fishmeal machine, be warming up to 60 ℃, ratio adding unit of activity in its amount 0.2% is the AS1 of 50000u/g, 398 neutral proteinases, enzymolysis is 20 minutes under the situation of stirring and 60 ℃ of temperature controls, wait it to be water-soluble state, adding is by feather albumen powder, wheat bran (rice bran), the auxiliary material absorption that the soya-bean cake powder is formed, the accessory formula percentage by weight is pressed the fresh scallops soft body: feather albumen powder: wheat bran or rice bran: soya-bean cake powder=60%: 7.5%: 7.5%: 25%, mix, fully adsorb the sticky material of enzymolysis Deng auxiliary material after, take out in cement threshing ground Exposure to Sunlight 24 hours, utilize ultraviolet ray in the sunlight to make a part in the auxiliary material in conjunction with albumen (lipoprotein, glycoprotein) decompose, further improve protein utilization and with big portion water evaporates, further be dried to moisture below 10% with fishmeal machine then, simultaneously wherein microorganism and insect are killed, pulverize in the moistureproof Polywoven Bag of packing into standard test warehousing after passing in accordance with regulations again with the pulverizer of Φ 2mm screen cloth.
With the fish is the manufacturing of the feed with aqueous soluble protein of raw material, generally is the enzyme that utilizes fish self, impels its protein structure to transform.And the shellfish in the marine organisms, because the vigor of oneself protease is very weak, only rely on the enzyme of self to be difficult to carry out the protein conversion, characteristics of the present invention, utilize exactly and add proteolytic enzyme, the institutional framework of scallop soft body and protein structure are changed, and adjust the amino acid ligand ratio by auxiliary material, make it adapt to the animal body needs, be beneficial to animal digesting and assimilating various compositions.Be convenient to transportation, store with the feed with aqueous soluble protein that this method is made, add sour preservative treatment owing to adopt, it is strong to have solved the scallop harvest season, scallop soft body amount has little time processing more or measures little inconvenient processing problems, and the contained various nutriments of scallop soft body are fully used and bring into play, be to solve the effective ways that the scallop soft body utilizes problem.
The feed with aqueous soluble protein of listing this manufactured in the accompanying drawing with feed with the scallop soft body dry powder of bright product convection drying pulverizing and fish meal small white mouse its separately protein digestibility absorb situation.
For advantage of the present invention and application in the art thereof are described preferably, describe below the better enforcement of selection of optimal fermentation condition of the present invention and product in detail.
1, the addition of hydrochloric acid in the preservative treatment process
New fresh scallops soft body adds appropriate hydrochloric acid and carries out preservative treatment, to be adapted to long-term processing after rubbing. The quality of antiseptic effect directly has influence on the quality of goods, and table 1 has been listed the antiseptic effect of regulating each control group after processing by different pH values.
Each control group antiseptic effect in the different pH value of table 1 situation
According to result of the test, under the prerequisite that guarantees storage effect, select higher pH value more reasonable for regulating the acid adding index, can use less like this hydrochloric acid can reach again the corrosion-resistant purpose, thereby more suitable as the index of control acid adding amount with pH value 2.5.
2, the control of enzyme concentration and hydrolysis temperature
The AS1 that the present invention selects, the peak enzymolysis-ability condition of 398 neutral proteinases is pH7,40 ℃, with the raw material of acid storage with CaO or Ca(OH)2After being adjusted to pH7, add this enzyme and under the temperature control condition, carry out enzymolysis, though the optimum temperature of this enzyme is 40 ℃, and this condition temperature reckons without time factor, carries out enzyme digestion reaction in this lower long period of temperature, certainly will cause the microorganism amount reproduction, for this reason need be by improving temperature, accelerate enzyme digestion reaction, suppress the effect of microorganism with high temperature, in order to carry out preferably the conversion of institutional framework and protein. Be cost reduction in addition, should reduce enzyme concentration as far as possible, take full advantage of and bring into play enzymatic activity. Table 2 has been listed scallop soft body institutional framework under the enzymatic hydrolysis conditions such as enzymolysis time, enzyme concentration, temperature control and the conversion situation of protein. As can be seen from Table 2, be the 0.2%-0.5% of raw material weight at enzyme concentration, hydrolysis temperature is 40 ℃-70 ℃, and enzymolysis time is in 10-90 minute, and three conditions of corresponding adjustment all can make the scallop soft body substantially finish the conversion of institutional framework and protein.
According to the control experiment result, under 40 ℃ or 50 ℃ of temperature control conditions, enzymolysis speed is not only slow, is prone to corruption simultaneously.And under the situation of 60 ℃ of enzyme concentrations 0.2%, just can finish the conversion process of tissue morphology and protein structure in 20 minutes, simultaneously because under 60 ℃ situation, most of putrefactive microorganisms is suppressed, therefore corruption can not appear in the whole process.
Embodiment:
1 ton of new fresh scallops soft body is rubbed, adds 36N food grade concentrated hydrochloric acid, be adjusted to pH2.5, stir store standby.Acid store before the enzymolysis raw material with CaO or Ca(OH)
2Regulate pH value to 7, be warmed up to 60 ℃ in the adding fishmeal machine, with the AS1 of raw material weight 0.2%, 398 neutral proteinases add with 50 ℃ warm water dissolving back, and constantly stirring, temperature control enzymolysis 20 minutes.Then with auxiliary material: 0.42 ton of 0.125 ton of soya-bean cake of wheat bran or rice bran, 0.125 ton of adding of feather albumen powder, mix and stir, make it fully to adsorb the material behind the enzymolysis, in cement threshing ground Exposure to Sunlight 24 hours, evaporate etc. most of moisture, then import in the fishmeal machine, its sterilizing and drying to moisture content of material below 10%, is taken out cooling, and with the pulverizer pulverizing of Φ 2mm screen cloth, be sub-packed in the 50kg composite plastic woven bag, and carry out product inspection, by the feed with aqueous soluble protein that said method obtains, its various nutritional labelings and content see Table 3, table 4
Can find out that from table 3, table 4 with the feed with aqueous soluble protein that this method is made, protein content height, necessary amino acid A wide selection of colours and designs is a kind of novel high-quality animal protein source.Because aqueous soluble protein contained in this feed need be through complicated digestion process, animal can more easily digest and absorb, and its actual feed support effect etc. and high-quality fish meal quite (seeing Table 5) table 5 listed the result of the test that the feed with aqueous soluble protein by this manufactured replaces the imported fish meal of crude protein content 62% to feed breeding egg chicken.
To storage performance and the security by the feed with aqueous soluble protein of this manufactured, testing result shows, can preserve more than 1 year under less than 10% situation at the goods water content and never degenerate, and the every sanitary index of these goods all conforms with hygienic requirements in addition.Is safe with the also no abnormal phenomenon event of its feeding animals feed with aqueous soluble protein as the animal protein nutrient source.
The present invention has good social benefit and economic benefit, is a kind of Perfected process that makes full use of this nutrient protein source of scallop soft body.
Each control group antiseptic effect under the different pH value of table 1 situation
Number 12345
PH value 2.0 2.5 2.5 2.5 2.8
After VBN change VBN change VBN becomes five days summers
Changing the little VBN rising of littleization of littleization pH value rises
Antiseptic effect
The anticorrosion effect of the anticorrosion effect of anticorrosion effect aerogenesis VBN occurs and changes greatly
The good denaturalization phenomenon denaturalization phenomenon of the good fruit of the good fruit of fruit is serious
Enzymolysis transforming degree contrast under each time of the different enzyme concentrations of table 2 and the temperature
40 ℃ 50 ℃ 60 ℃ 70 ℃ of temperature
Time (minute) 30 60 90 20 40 60 10 20 30 10 20 30
Enzyme concentration
0 / / / / / / - - - - - -
0.1% / / / / / / + + ++ ++ ++ ++
0.2%+ + + ++ + ++ +++ ++ +++ +++ ++ +++ +++
0.5% + ++ +++ + ++ +++ ++ +++ +++ +++ +++ +++
Annotate: "/" expression is surveyed "-" expression and is changed less "+" expression " ++ " expression that changes slightly and have and one transform " +++" expression to finish conversion substantially
The general composition unit of table 3 feed with aqueous soluble protein: percentage (%)
Project moisture crude protein crude fat ash content crude fibre+NFE
Content 9.7 44.1 6.4 7.4 32.4
Table 5 feed with aqueous soluble protein import substitution fish meal (crude protein 62%)
The Chinese mugwort of feeding is dialled breeding laying hen experimental result increasingly
Group test group control group
(add 8% aqueous soluble protein (adding 8% imported fish meal)
The project feed)
Have a try and test (only) 250+25 1600+175 just
Test hen cock hen cock
Phase
Dead test end (only) 265 1707
Die
Feelings death toll (only) 10 68
The condition death rate (%) 3.6 3.8
Try total feed consumption (jin) 8652.3 54890
Test
Phase
Average day feed consumption (gram) 170 169.5 of consumption
Material
Feelings
Every total feed consumption of condition (jin) 31.5 31.2
Examination
Always test and lay eggs (piece) 14,950 91264
Phase
Produce and on average only to lay eggs (piece) 60 57
Egg
Feelings
Condition laying rate (%) 65 62
Examination
Test every and always lay eggs (piece) 60 57
Phase
Raise every total feed consumption (jin) 31.5 31.2
Material
Imitate
Rate egg material is than (piece/jin) 1.90 1.82
Claims (3)
1, a kind of method of making feed with aqueous soluble protein with the scallop soft body, the AS1,398 neutral proteinases that it is characterized in that will accounting for earlier raw material weight 0.2%-0.5% add in the scallop soft body of rubbing, carrying out temperature control decomposes, temperature when it decomposes is controlled at 40 ℃-70 ℃, time is 10-90 minute, and then carry out adsorption dry, and adjust the amino acid ligand ratio with auxiliary material.
2,, it is characterized in that said auxiliary material is a feather albumen powder, wheat bran or rice bran and soya-bean cake powder according to the method for the said manufacturing feed with aqueous soluble protein of claim 1.
3,, it is characterized in that the accessory formula percentage by weight is by the fresh scallops soft body: feather albumen powder: wheat bran (rice bran): soya-bean cake powder=60%: 7.5%: 7.5%: 25% according to the said auxiliary material of claim 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN89105308A CN1022538C (en) | 1989-06-05 | 1989-06-05 | Method for preparing feed with aqueous soluble protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN89105308A CN1022538C (en) | 1989-06-05 | 1989-06-05 | Method for preparing feed with aqueous soluble protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1037823A CN1037823A (en) | 1989-12-13 |
| CN1022538C true CN1022538C (en) | 1993-10-27 |
Family
ID=4856073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN89105308A Expired - Fee Related CN1022538C (en) | 1989-06-05 | 1989-06-05 | Method for preparing feed with aqueous soluble protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1022538C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05336896A (en) * | 1992-04-06 | 1993-12-21 | Ajinomoto Co Inc | Feed composition for pig dam |
| CN105767002B (en) * | 2016-05-16 | 2018-07-27 | 济宁市海洋农化厂 | Aluminum phosphate expelling mouse agent packaging structure and administration device convenient for Slag recovering |
| CN115843929B (en) * | 2020-09-02 | 2024-08-02 | 山东普兴生物科技有限公司 | Application of compound polypeptide feed in regulation and control of chicken ovary granulosa cells |
-
1989
- 1989-06-05 CN CN89105308A patent/CN1022538C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1037823A (en) | 1989-12-13 |
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