CN112106653A - Intelligent pleurotus eryngii breeding system and method - Google Patents
Intelligent pleurotus eryngii breeding system and method Download PDFInfo
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- CN112106653A CN112106653A CN202010910416.2A CN202010910416A CN112106653A CN 112106653 A CN112106653 A CN 112106653A CN 202010910416 A CN202010910416 A CN 202010910416A CN 112106653 A CN112106653 A CN 112106653A
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention provides an intelligent pleurotus eryngii breeding system, which comprises the following steps: (1) preparing a culture dish: preparing a culture medium; (2) and (3) breeding: uniformly smearing the strain spores prepared by preselection in corresponding culture dishes, and numbering A, B and C for each culture dish; (3) performing antagonistic selection; (4) cultivation: the dominant species are inoculated in a cultivation main body filled with a culture material. (5) Picking: and picking the mature pleurotus eryngii. According to the invention, the opening of the cultivation bottle is divided into areas, so that the growth and the harvesting of pleurotus eryngii are facilitated, the separation device and the cultivation bottle are conveniently and quickly matched, the working efficiency is improved, the picking device is used for harvesting the mature pleurotus eryngii, the labor force is saved, the working efficiency is improved, and the labor cost is reduced. The invention also provides an intelligent pleurotus eryngii breeding method.
Description
Technical Field
The invention relates to the technical field of pleurotus eryngii cultivation, in particular to an intelligent pleurotus eryngii breeding system and method.
Background
The pleurotus eryngii is a new rare edible fungus variety integrating edible, medicinal and dietary therapy. The pleurotus eryngii has fleshy flesh, crisp and tender texture, particularly has compact, solid and milky stem tissues, can be completely eaten, has more crisp and smooth stem than pileus and is tasty and refreshing, is called as oyster mushroom king and dried oyster mushroom, has pleasant almond fragrance and taste like abalone, and is suitable for fresh keeping and processing.
The traditional cultivation method for pleurotus eryngii is characterized in that the pleurotus eryngii is cultivated through a cultivation bag, an independent area is not provided for the growth of the pleurotus eryngii, the pleurotus eryngii is easy to squeeze together when growing, the growth of the pleurotus eryngii and the collection of subsequent workers are not facilitated, manual collection is relied on, the working efficiency is unstable, if the pleurotus eryngii is not thoroughly sterilized before entering a pleurotus eryngii discharging room, the pleurotus eryngii is easily infected, waste mushrooms are caused, and waste is caused; in addition, the growth of the pleurotus eryngii is greatly influenced by the environment, the pleurotus eryngii with poor quality is difficult to grow normally, and the yield is not high.
Accordingly, the present inventors have made extensive studies to solve the above problems and have made the present invention.
Disclosure of Invention
The invention aims to provide an intelligent pleurotus eryngii breeding system to solve the problems that manual harvesting and harvesting efficiency is low, a cultivation bag does not have an independent area for pleurotus eryngii to grow, and the pleurotus eryngii is easy to be gradually crowded together in the growth process of the pleurotus eryngii in the background art.
The invention also aims to provide an intelligent pleurotus eryngii breeding method to solve the problem of poor quality of pleurotus eryngii in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
an intelligent pleurotus eryngii breeding system comprises the following steps:
s1, preparing culture dishes, preparing a culture medium, pouring the prepared culture medium into a sealed bottle, sealing the mouth of the sealed bottle, sterilizing the sealed bottle, cooling the sealed bottle, and pouring the culture medium in the sealed bottle into a plurality of culture dishes which are sterilized in advance;
s2, breeding, namely uniformly smearing the strain spores prepared by preselection on corresponding culture dishes, and numbering A, B and C on each culture dish;
a: blow-drying in an aseptic state to form a bacterial membrane on the surface, injecting nitrogen ion beams, washing the bacterial membrane subjected to mutagenesis treatment with sterile water, coating the diluted bacterial membrane on a culture dish, selecting a single strain with good growth to inoculate the single strain in the culture dish, and selecting a high-yield strain with stable genetic character after culture;
no. B: under the aseptic condition, a new strain of edible fungi is bred by a monospore cross breeding technology, the well-grown new strain is inoculated in a culture dish, and a high-yield strain with stable hereditary character is selected after culture;
no. C: under the aseptic condition, mutation of spore genes of the strains is realized by adopting an ultraviolet irradiation mutagenesis method, the mutated spores of the strains are coated on a culture dish, a single strain with good growth is selected and inoculated in the culture dish, and a high-yield strain with stable hereditary character is selected after culture;
s3, antagonistic selection, namely, mixing and inoculating the three dominant strains selected by the three methods on a culture dish, culturing at a proper temperature for a preset time, taking out the culture dish, and observing the dominant strains;
s4, inoculating dominant strains into a cultivation container filled with compost, conveying the cultivation container into a fungus cultivation chamber, dividing an opening of the cultivation container into a plurality of areas after fungus emergence, and transferring the cultivation container to a fruiting room to fruiting;
s5, picking, namely picking the mature pleurotus eryngii.
Further, in S4, the opening of the cultivation container is partitioned into a plurality of areas by a partition device.
Further, the separating means comprises a first separating element separating regions of the mouth in a first direction and a second separating element separating regions of the mouth in a second direction.
Further, the first separating component comprises a first applicator roll for applying glue in a first direction of the opening, a first lifting driving device for driving the first applicator roll to move up and down, and a first reciprocating driving device for driving the first applicator roll to reciprocate towards the first direction; a first adhesive tape stretched along a first direction is arranged on the first applicator roll; the second separating component comprises a second applicator roll for applying glue to the second direction of the opening, a second lifting driving device for driving the second applicator roll to move up and down, and a second reciprocating driving device for driving the second applicator roll to reciprocate towards the second direction; and a second adhesive tape stretched along a second direction is arranged on the second applicator roll.
Further, the separating device also comprises a cutting device for cutting the first adhesive tape and the second adhesive tape.
Further, the cutting device comprises a first cutting assembly for cutting the first adhesive tape on the first applicator roll and a second cutting assembly for cutting the second adhesive tape on the second applicator roll.
Further, the first shearing assembly comprises a first cutter, a second cutter and a first opening and closing driving device for driving the first cutter and the second cutter to open and close; the second shearing assembly comprises a third cutter, a fourth cutter and a second opening and closing driving device for driving the third cutter and the fourth cutter to open and close.
Further, the cultivation container includes a cultivation bottle.
Furthermore, a first separation groove for the first adhesive tape to pass through and a second separation groove for the second adhesive tape to pass through are formed on the bottleneck of the cultivation bottle.
Further, in S4, the cultivation bottles are placed in order on a cultivation rack, and the cultivation bottles are transported to a fruiting room through the cultivation rack for fruiting.
Further, the cultivation frame comprises a containing part for containing the cultivation bottles.
Furthermore, a plurality of accommodating holes for accommodating the cultivation bottles and fixing the cultivation bottles are formed in the upper surface of the accommodating part, and the accommodating holes are uniformly distributed in the accommodating part.
Further, the upper surface of the containing part forms a containing cavity for containing the cultivation bottle.
Further, the upper surface of the accommodating cavity is provided with a fixing part for fixing the cultivation bottle.
Further, the fixing member includes a fixing ring.
Further, the fixing ring is fixedly connected to the upper surface of the accommodating cavity.
Furthermore, the fixing rings are uniformly arranged on the accommodating cavity.
Furthermore, the edge of the containing part is also provided with a sticking part which is in one-to-one correspondence with the first adhesive tape and the second adhesive tape.
Further, the pasting part comprises a pasting block.
Further, the cultivation frame also comprises a frame body for bearing and containing the parts.
Furthermore, an accommodating groove for accommodating the accommodating part is formed on the side surface of the frame body; the holding part is mounted on the holding groove.
Further, hold part and holding tank pull formula sliding connection.
Further, the holding tank of support body is equipped with a plurality of parts that hold from last equidistant down.
Further, the bottom of the frame body is provided with universal wheels.
Further, in S4, the compost is wood chip compost.
Further, the wood chip compost comprises a main material and an auxiliary material.
Further, the main material comprises wood dust.
Further, the weight percentage of the miscellaneous wood chips in the wood chip compost is 70-80%.
Further, the wood dust accounts for 75% of the wood dust culture material in percentage by weight.
Further, the auxiliary materials comprise bran, sugar, lime and calcium carbonate.
Further, the auxiliary materials account for 20-30% of the wood chip compost in percentage by weight.
Further, the wood dust, the bran, the sugar, the lime and the calcium carbonate are mixed according to the weight percentage of 75%, 22%, 1% and 1%.
Further, the water content of the wood chip compost is 63%.
Further, in S4, the inoculated culture bottle is transferred to a culture room having a temperature of 24 ℃ and a relative air humidity of not more than 70%, and the culture room is kept in a dark state.
Further, in S5, the mature pleurotus eryngii is subjected to a picking process by a picking device.
Furthermore, the picking device comprises harvesting parts which correspond to the cultivation frames one by one, and a third opening and closing driving device for driving the harvesting parts to open and close.
Further, the harvesting part comprises a first harvesting knife, a second harvesting knife, a third harvesting knife and a mounting head, wherein the first harvesting knife, the second harvesting knife, the third harvesting knife and the mounting head are used for harvesting mature pleurotus eryngii; the first harvesting knife, the second harvesting knife and the third harvesting knife are arranged around the mounting head.
Further, the picking device also comprises a moving driving device for driving the picking part to move, and a third lifting driving device for driving the picking part to lift.
An intelligent pleurotus eryngii breeding method comprises the following steps:
(1) preparing culture dishes, preparing a culture medium, pouring the prepared culture medium into a sealed bottle, sealing the mouth of the sealed bottle, sterilizing the sealed bottle, cooling the sealed bottle, and pouring the culture medium in the sealed bottle into a plurality of culture dishes which are sterilized in advance;
(2) selecting and breeding, namely uniformly smearing the strain spores prepared by preselection in corresponding culture dishes, and numbering A, B and C for each culture dish;
a: blow-drying in an aseptic state to form a bacterial membrane on the surface, injecting nitrogen ion beams, washing the bacterial membrane subjected to mutagenesis treatment with sterile water, coating the diluted bacterial membrane on a culture dish, selecting a single strain with good growth to inoculate the single strain in the culture dish, and selecting a high-yield strain with stable genetic character after culture;
no. B: under the aseptic condition, a new strain of edible fungi is bred by a monospore cross breeding technology, the well-grown new strain is inoculated in a culture dish, and a high-yield strain with stable hereditary character is selected after culture;
no. C: under the aseptic condition, mutation of spore genes of the strains is realized by adopting an ultraviolet irradiation mutagenesis method, the mutated spores of the strains are coated on a culture dish, a single strain with good growth is selected and inoculated in the culture dish, and a high-yield strain with stable hereditary character is selected after culture;
(3) and (3) antagonistic selection, namely, mixing and inoculating the three dominant strains selected by the three methods on a culture dish, culturing at a proper temperature for a preset time, taking out the culture dish, and observing to obtain the dominant strains.
Further, in the step (1), the medium is a wheat seed medium.
Further, the wheat seed culture medium comprises a main material and an auxiliary material.
Further, the main material comprises wheat grains.
Further, the wheat grains account for 85-90% of the wheat seed culture medium by weight.
Further, the wheat grains account for 88 percent of the weight of the wheat grain culture medium.
Further, the auxiliary materials comprise rice powder and gypsum.
Further, the auxiliary materials account for 10-15% of the wheat seed culture medium by weight.
Further, the wheat grains, the rice powder and the gypsum are mixed according to the weight percentage of 88 percent, 10 percent and 2 percent, and the PH value is 7.5-8.
After adopting the structure, the intelligent pleurotus eryngii breeding system has the following beneficial effects:
the cultivation bottles are placed on the cultivation frame in order, workers can conveniently transport the cultivation bottles to a fruiting room, the use is convenient and fast, meanwhile, the separation device can simultaneously carry out separation area treatment on the cultivation bottles on the cultivation frame, the first adhesive tape can separate areas of a plurality of cultivation bottles at the same transverse horizontal position through the first separation part, the second separation part can separate areas of a plurality of cultivation bottles at the same longitudinal horizontal position through the second adhesive tape, and the first separation part and the second separation part can simultaneously carry out separation area operation on the plurality of cultivation bottles at one time, so that the bottleneck separation area of the cultivation bottles is high in speed and efficiency, the trouble of manually pasting the adhesive tape is avoided, and the labor cost is reduced; meanwhile, the pleurotus eryngii grows in the corresponding areas, the pleurotus eryngii is prevented from being squeezed together when growing, the follow-up harvesting work of the mature pleurotus eryngii is facilitated, the mature pleurotus eryngii is harvested through the picking device, the labor force is saved, the harvesting efficiency is improved, and the labor cost is reduced.
The invention also provides an intelligent pleurotus eryngii breeding method, which is characterized in that strain spores are bred in three breeding modes of A, B and C, antagonistic selection is adopted, dominant strains with stable genetic characters are selected, the quality of pleurotus eryngii is improved, and the survival rate of pleurotus eryngii is higher.
Drawings
FIG. 1 is a schematic perspective view of an intelligent Pleurotus eryngii breeding system and method according to the present invention;
FIG. 2 is a schematic diagram of the position structure of a cultivation frame, a separation device, a picking device and a cultivation bottle of the intelligent pleurotus eryngii breeding system and method;
FIG. 3 is a schematic structural diagram of a separation device for separating cultivation bottles into separate areas according to the intelligent Pleurotus eryngii breeding system and method of the present invention;
FIG. 4 is a schematic perspective view of a cultivation bottle of the intelligent Pleurotus eryngii breeding system and method of the present invention;
FIG. 5 is a schematic perspective view of a picking device of the intelligent Pleurotus eryngii breeding system and method of the present invention.
In the figure: 1-a separation device, 11-a first separation part, 12-a second separation part, 111-a first applicator roll, 112-a first adhesive tape, 13-a cutting device, 131-a first shearing component, 132-a second shearing component, 1311-a first cutter, 1312-a second cutter, 1313-a first opening and closing driving device, 2-a cultivation bottle, 21-a bottle neck, 211-a first separation groove, 212-a second separation groove, 3-a cultivation frame, 31-a containing part, 311-a containing cavity, 312-a pasting part, 32-a frame body, 321-a containing groove, 322-a universal wheel, 4-a picking device, 41-a harvesting part, 42-a third opening and closing driving device, 411-a first harvesting knife, 412-a second harvesting knife, 413-a third harvesting knife, 414-mounting head, 5-mushroom house.
Detailed Description
In order to further explain the technical solution of the present invention, the following detailed description is given by way of specific examples.
As shown in fig. 1 to 5, the intelligent pleurotus eryngii breeding system of the present invention comprises the following steps:
s1, preparing culture dishes, preparing a culture medium, pouring the prepared culture medium into a sealed bottle, sealing the mouth of the sealed bottle, sterilizing the sealed bottle, cooling the sealed bottle, and pouring the culture medium in the sealed bottle into a plurality of culture dishes which are sterilized in advance;
s2, breeding, namely uniformly smearing the strain spores prepared by preselection on corresponding culture dishes, and numbering A, B and C on each culture dish;
a: blow-drying in an aseptic state to form a bacterial membrane on the surface, injecting nitrogen ion beams, washing the bacterial membrane subjected to mutagenesis treatment with sterile water, coating the diluted bacterial membrane on a culture dish, selecting a single strain with good growth to inoculate the single strain in the culture dish, and selecting a high-yield strain with stable genetic character after culture;
no. B: under the aseptic condition, a new strain of edible fungi is bred by a monospore cross breeding technology, the well-grown new strain is inoculated in a culture dish, and a high-yield strain with stable hereditary character is selected after culture;
no. C: under the aseptic condition, mutation of spore genes of the strains is realized by adopting an ultraviolet irradiation mutagenesis method, the mutated spores of the strains are coated on a culture dish, a single strain with good growth is selected and inoculated in the culture dish, and a high-yield strain with stable hereditary character is selected after culture;
s3, antagonistic selection, namely, mixing and inoculating the three dominant strains selected by the three methods on a culture dish, culturing at a proper temperature for a preset time, taking out the culture dish, and observing the dominant strains;
s4, inoculating dominant strains into a cultivation container filled with compost, conveying the cultivation container into a fungus cultivation chamber, dividing an opening of the cultivation container into a plurality of areas after fungus emergence, and conveying the cultivation container to a fruiting room 5 to fruiting;
s5, picking, namely picking the mature pleurotus eryngii.
In this way, the cultivation bottles 2 are placed on the cultivation frame 3 in order, workers can conveniently transport the cultivation bottles 2 to the fruiting room 5, the use is convenient and fast, meanwhile, the separation device 1 simultaneously carries out separation area treatment on the cultivation bottles 2 on the cultivation frame 3, the first adhesive tape 112 is used for separating the areas of a plurality of cultivation bottles 2 at the same transverse horizontal position through the first separation part 11, the second adhesive tape is used for separating the areas of a plurality of cultivation bottles 2 at the same longitudinal horizontal position through the second separation part 12, and the first separation part 11 and the second separation part 12 are used for simultaneously carrying out separation area operation on the plurality of cultivation bottles 2 at one time, so that the bottleneck separation area of the cultivation bottles 2 is high in speed and high in efficiency, the trouble of manually sticking adhesive tapes is avoided, and the labor cost is reduced; meanwhile, the pleurotus eryngii grows in the corresponding areas, the pleurotus eryngii is prevented from being squeezed together when growing, the follow-up harvesting work of the mature pleurotus eryngii is facilitated, the mature pleurotus eryngii is harvested through the picking device 4, the labor force is saved, the harvesting efficiency is improved, and the labor cost is reduced.
Preferably, in S4, the opening of the cultivation container is partitioned into a plurality of areas by the partitioning device 1. The opening of the cultivation container is divided into a plurality of areas at one time through the separating device 1, the efficiency of dividing the opening of the cultivation container into the plurality of areas is improved, the pleurotus eryngii grows in the corresponding areas, the growth of the pleurotus eryngii is facilitated, and convenience is brought to the subsequent picking process.
Preferably, the partition 1 comprises a first partition element 11, which partitions a first direction of the mouth of the bottle, and a second partition element 12, which partitions a second direction of the mouth of the bottle. The first partition member 11 partitions the plurality of cultivation containers in the horizontal direction, and the second partition member 12 partitions the plurality of cultivation containers in the vertical direction, so that the mouths of the cultivation containers form areas in which pleurotus eryngii grows independently.
Preferably, the first separating member 11 includes a first applicator roller 111 applying glue in a first direction of the opening, a first elevation driving device driving the first applicator roller 111 to move up and down, and a first reciprocation driving device driving the first applicator roller 111 to reciprocate in the first direction; a first adhesive tape 112 stretched along a first direction is arranged on the first applicator roll 111; the second partition member 12 includes a second applicator roll for applying glue in a second direction of the opening, a second lifting drive device for driving the second applicator roll to move up and down, and a second reciprocating drive device for driving the second applicator roll to reciprocate in the second direction; and a second adhesive tape stretched along a second direction is arranged on the second applicator roll. After the first lifting driving device is driven to downwards reach a preset position of the cultivation container, the first adhesive tape 112 is stuck on the opening by downwards pressing, the first reciprocating driving device drives the first applicator roll 111 to transversely move, and the opening of the cultivation container which is transversely and orderly placed is divided into an upper area and a lower area by the first adhesive tape 112; after the second lifting driving device is driven to downwards reach a preset position of the cultivation container, the second lifting driving device presses downwards to enable the second adhesive tape to be adhered to the opening, and the second reciprocating driving device drives the second applicator roll to move longitudinally, so that the opening of the cultivation container which is placed orderly longitudinally is divided into a left area and a right area by the second adhesive tape; after the first and second partition members 11 and 12 partition the opening of the cultivation container into regions, respectively, the opening of the cultivation container becomes a region in which pleurotus eryngii grows independently.
Preferably, in order to make the work of dividing the area smoother, the dividing device 1 further includes a cutting device 13 for cutting the first tape 112 and the second tape.
Preferably, the severing device 13 comprises a first cutting assembly 131 to cut the first tape 112 on the first applicator roll 111, and a second cutting assembly 132 to cut the second tape on the second applicator roll. The first adhesive tape 112 is cut by the first cutting assembly 131, and the second adhesive tape is cut by the second cutting assembly 132, thereby completing the whole process of the tape separating operation.
Preferably, the first cutting assembly 131 comprises a first cutter 1311, a second cutter 1312, and a first opening and closing driving device 1313 for driving the first cutter 1311 and the second cutter 1312 to open and close; the second cutting assembly 132 includes a third cutter, a fourth cutter, and a second opening/closing driving device for driving the third cutter and the fourth cutter to open/close. The first opening and closing driving device 1313 drives the first cutter 1311 and the second cutter 1312 to close, and cuts off the first adhesive tape 112; the second opening and closing driving device drives the third cutter and the fourth cutter to be closed, and the second adhesive tape is cut off.
Preferably, the cultivation container comprises a cultivation bottle 2. The growing environment of the pleurotus eryngii is more stable through the cultivation bottle 2.
Preferably, the neck 21 of the cultivation bottle 2 is formed with a first partition groove 211 through which the first tape 112 passes and a second partition groove 212 through which the second tape passes. The first adhesive tape 112 is fixed by the first separating groove 211, and the second adhesive tape is fixed by the second separating groove 212, so that the structure is more stable when the first adhesive tape 112 and the second adhesive tape are adhered on the bottle mouth.
Preferably, in S4, the cultivation bottles 2 are placed in order on the cultivation shelves 3, and the cultivation bottles 2 are transported to the fruiting room 5 through the cultivation shelves 3 for fruiting. Through putting cultivation bottle 2 neatly on cultivation frame 3, improve space utilization to and be convenient for carry cultivation bottle 2 to go out mushroom 5 and go out the mushroom.
Preferably, the cultivation shelf 3 includes a holding part 31 for holding the cultivation bottles 2. The cultivation bottle 2 is placed through the placing part 31, so that the cultivation bottle 2 is not easy to be knocked off.
Preferably, a plurality of receiving holes for receiving and fixing the cultivation bottles 2 are formed on the upper surface of the receiving part 31, and the receiving holes are uniformly arranged on the receiving part 31. Fix cultivation bottle 2 through the holding pore pair, make cultivation bottle 2 be difficult for taking place the slope and overturn, the separator 1 of being convenient for simultaneously separates regional operation to cultivation bottle 2.
Preferably, the upper surface of the accommodating part 31 forms an accommodating cavity 311 for accommodating the cultivation bottle 2. The cultivation bottle 2 is placed in the containing cavity 311, so that the cultivation bottle 2 is not easy to incline and overturn.
Preferably, the upper surface of the receiving cavity 311 is provided with a fixing member for fixing the cultivation bottle 2. The cultivation bottle 2 is fixed in the containing cavity 311 through the fixing component, and meanwhile, the separation device 1 is convenient for separating the cultivation bottle 2 into areas.
Preferably, the fixing member includes a fixing ring for facilitating the attachment and detachment of the cultivation bottle 2.
Preferably, the fixing ring is fixedly connected to the upper surface of the accommodating cavity 311 for facilitating the assembly and disassembly of the cultivation bottle 2.
Preferably, in order to facilitate the operation of the partition device 1 for partitioning the bottle mouth of the cultivation bottle 2 into areas, the fixing rings are uniformly arranged on the accommodating cavity 311.
Preferably, in order to facilitate the operation of dividing the mouth of the cultivation bottle 2 by the first adhesive tape 112 and the second adhesive tape, the edge of the containing part 31 is further provided with an adhering part 312 corresponding to the first adhesive tape 112 and the second adhesive tape one by one.
Preferably, the attachment portion 312 comprises an attachment patch. The first adhesive tape 112 and the second adhesive tape are adhered to the adhesive blocks and then drag the adhesive tapes towards the corresponding directions, and the first adhesive tape 112 and the second adhesive tape are used as force points through the adhesive blocks, so that the first adhesive tape 112 and the second adhesive tape are smoother when the areas of the bottle mouth are separated.
Preferably, the cultivation shelf 3 further includes a shelf body 32 carrying the containing part 31. The containing parts 31 are placed on the frame body 32, so that the containing parts 31 can be conveniently put in and taken out, and the space utilization rate is improved.
Preferably, a receiving groove 321 for receiving the accommodating part 31 is formed on a side surface of the frame body 32; the containing part 31 is mounted on the receiving groove 321. The containing part 31 is mounted on the receiving groove 321. The containing part 31 is convenient to put in and take out, and the space utilization rate is improved.
Preferably, the holding part 31 and the receiving groove 321 are slidably connected in a drawing manner. The holding part 31 can be conveniently, quickly, stably and smoothly placed in and taken out of the frame body 32.
Preferably, the accommodating groove 321 of the frame body 32 is provided with a plurality of accommodating parts 31 at equal intervals from top to bottom. Through a plurality of parts 31 that hold, improve space utilization, make the room of fruiting 5 hold more cultivation bottles 2 that supply the growth of apricot bao mushroom.
Preferably, universal wheels 322 are provided at the bottom of the frame body 32. Through the setting of universal wheel 322, make cultivation frame 3 more easily removed the room of fruiting 5 in, improve work efficiency, alleviate staff's burden.
Preferably, in S4, the compost is wood chip compost. The wood chip culture material has short culture time, low cost and easily obtained raw materials.
Preferably, in order to make the culture effect of the culture material better, the wood chip culture material comprises a main material and an auxiliary material.
Preferably, the main material comprises wood shavings. The culture of the miscellaneous wood chips requires short time, and has low cost and easily obtained raw materials.
Preferably, in order to ensure that the culture effect of the culture material is better, the wood dust accounts for 70-80% of the wood dust culture material by weight.
Preferably, in order to improve the culture effect of the culture material, the wood dust accounts for 75% of the wood dust culture material.
Preferably, in order to make the culture effect of the culture material better, the auxiliary materials comprise bran, sugar, lime and calcium carbonate.
Preferably, in order to ensure that the culture effect of the culture material is better, the auxiliary materials account for 20-30% of the wood chip culture material by weight.
Preferably, the sawdust, the bran, the sugar, the lime and the calcium carbonate are mixed according to the weight percentage of 75%, 22%, 1% and 1% in order to make the culture effect of the culture material better.
Preferably, in order to make the culture effect of the culture material better, the water content of the wood chip culture material is 63%.
Preferably, in order to improve the effect of cultivating bacteria in the cultivating chamber, the inoculated cultivation bottle 2 is transferred to the cultivating chamber with a temperature of 24 ℃ and a relative humidity of air of not more than 70% in S4, and the cultivating chamber is kept in a dark state.
Preferably, in S5, the mature pleurotus eryngii is subjected to a picking process by the picking means 4. Through picking device 4, improve and pick efficiency.
Preferably, the picking device 4 includes a pair of harvesting parts 41 corresponding to the cultivation shelves 3 one by one, and a third opening and closing driving device 42 that drives the harvesting parts 41 to open and close. The third opening and closing driving device 42 drives the harvesting part 41 to open and close, and mature pleurotus eryngii is harvested one by one.
Preferably, the harvesting part 41 includes a first harvesting knife 411, a second harvesting knife 412, a third harvesting knife 413 and a mounting head 414 for harvesting the mature pleurotus eryngii; the first collecting blade 411, the second collecting blade 412, and the third collecting blade 413 are provided around the mounting head 414. The pleurotus eryngii is cut off and harvested through the first harvesting knife 411, the second harvesting knife 412 and the third harvesting knife 413, and the knife edges are smooth, so that the pleurotus eryngii can be conveniently trimmed subsequently.
Preferably, the picking device 4 further comprises a moving driving device for driving the picking part 41 to move, and a third lifting driving device for driving the picking part 41 to lift. The harvesting part 41 is driven to be above each cultivation bottle 2 through the moving driving device, then the cultivation bottles 2 descend to harvest mature pleurotus eryngii in each cultivation bottle 2, the harvesting part 41 is driven to ascend and descend to the position of each containing part 31 through the third lifting driving device, and the pleurotus eryngii in each containing part 31 is harvested.
An intelligent pleurotus eryngii breeding method comprises the following steps:
(1) preparing culture dishes, preparing a culture medium, pouring the prepared culture medium into a sealed bottle, sealing the mouth of the sealed bottle, sterilizing the sealed bottle, cooling the sealed bottle, and pouring the culture medium in the sealed bottle into a plurality of culture dishes which are sterilized in advance;
(2) selecting and breeding, namely uniformly smearing the strain spores prepared by preselection in corresponding culture dishes, and numbering A, B and C for each culture dish;
a: blow-drying in an aseptic state to form a bacterial membrane on the surface, injecting nitrogen ion beams, washing the bacterial membrane subjected to mutagenesis treatment with sterile water, coating the diluted bacterial membrane on a culture dish, selecting a single strain with good growth to inoculate the single strain in the culture dish, and selecting a high-yield strain with stable genetic character after culture;
no. B: under the aseptic condition, a new strain of edible fungi is bred by a monospore cross breeding technology, the well-grown new strain is inoculated in a culture dish, and a high-yield strain with stable hereditary character is selected after culture;
no. C: under the aseptic condition, mutation of spore genes of the strains is realized by adopting an ultraviolet irradiation mutagenesis method, the mutated spores of the strains are coated on a culture dish, a single strain with good growth is selected and inoculated in the culture dish, and a high-yield strain with stable hereditary character is selected after culture;
(3) and (3) antagonistic selection, namely, mixing and inoculating the three dominant strains selected by the three methods on a culture dish, culturing at a proper temperature for a preset time, taking out the culture dish, and observing to obtain the dominant strains.
Therefore, the strain spores are bred through three breeding modes of A, B and C, and then antagonistic selection is adopted to select dominant strains with stable genetic characters, so that the quality of pleurotus eryngii is improved, and the survival rate of the pleurotus eryngii is higher.
Preferably, in step (1), the medium is a wheat seed medium. The wheat seed culture medium needs short time, and has low cost and easily obtained raw materials.
Preferably, the wheat seed culture medium comprises a main material and an auxiliary material in order to make the culture effect of the culture medium better.
Preferably, the main material comprises wheat grains for better culture effect of the culture medium.
Preferably, the wheat grains account for 85-90% of the wheat seed culture medium by weight in order to make the culture effect of the culture medium better.
Preferably, in order to make the culture effect of the culture medium better, the wheat grains account for 88% of the wheat seed culture medium by weight.
Preferably, in order to make the culture effect of the culture medium better, the auxiliary materials comprise rice powder and gypsum.
Preferably, in order to make the culture effect of the culture medium better, the auxiliary materials account for 10-15% of the weight of the wheat seed culture medium.
Preferably, in order to make the culture effect of the culture medium better, the wheat grains, the rice flour and the gypsum are mixed according to the weight percentage of 88 percent, 10 percent and 2 percent, and the pH value is 7.5-8.
The product form of the present invention is not limited to the embodiments and examples shown in the present application, and any suitable changes or modifications of the similar ideas should be made without departing from the patent scope of the present invention.
Claims (10)
1. An intelligent pleurotus eryngii breeding system is characterized by comprising the following steps:
s1, preparing culture dishes, preparing a culture medium, pouring the prepared culture medium into a sealed bottle, sealing the mouth of the sealed bottle, sterilizing the sealed bottle, cooling the sealed bottle, and pouring the culture medium in the sealed bottle into a plurality of culture dishes which are sterilized in advance;
s2, breeding, namely uniformly smearing the strain spores prepared by preselection on corresponding culture dishes, and numbering A, B and C on each culture dish;
a: blow-drying in an aseptic state to form a bacterial membrane on the surface, injecting nitrogen ion beams, washing the bacterial membrane subjected to mutagenesis treatment with sterile water, coating the diluted bacterial membrane on a culture dish, selecting a single strain with good growth to inoculate the single strain in the culture dish, and selecting a high-yield strain with stable genetic character after culture;
no. B: under the aseptic condition, a new strain of edible fungi is bred by a monospore cross breeding technology, the well-grown new strain is inoculated in a culture dish, and a high-yield strain with stable hereditary character is selected after culture;
no. C: under the aseptic condition, mutation of spore genes of the strains is realized by adopting an ultraviolet irradiation mutagenesis method, the mutated spores of the strains are coated on a culture dish, a single strain with good growth is selected and inoculated in the culture dish, and a high-yield strain with stable hereditary character is selected after culture;
s3, antagonistic selection, namely, mixing and inoculating the three dominant strains selected by the three methods on a culture dish, culturing at a proper temperature for a preset time, taking out the culture dish, and observing the dominant strains;
s4, inoculating dominant strains into a cultivation container filled with compost, conveying the cultivation container into a fungus cultivation chamber, dividing an opening of the cultivation container into a plurality of areas after fungus emergence, and transferring the cultivation container to a fruiting room to fruiting;
s5, picking, namely picking the mature pleurotus eryngii.
2. The intelligent pleurotus eryngii breeding system according to claim 1, wherein: in S4, the opening of the cultivation container is partitioned into a plurality of areas by a partition device.
3. The intelligent pleurotus eryngii breeding system according to claim 2, wherein: the partition device includes a first partition member partitioning a region in a first direction of the opening, and a second partition member partitioning a region in a second direction of the opening.
4. The intelligent pleurotus eryngii breeding system according to claim 3, wherein: the first separating component comprises a first applicator roll for applying glue in a first direction of the opening, a first lifting driving device for driving the first applicator roll to move up and down, and a first reciprocating driving device for driving the first applicator roll to reciprocate towards the first direction; a first adhesive tape stretched along a first direction is arranged on the first applicator roll; the second separating component comprises a second applicator roll for applying glue to the second direction of the opening, a second lifting driving device for driving the second applicator roll to move up and down, and a second reciprocating driving device for driving the second applicator roll to reciprocate towards the second direction; and a second adhesive tape stretched along a second direction is arranged on the second applicator roll.
5. The intelligent pleurotus eryngii breeding system according to claim 4, wherein: the separating device further comprises a cutting device for cutting the first adhesive tape and the second adhesive tape.
6. The intelligent pleurotus eryngii breeding system according to claim 5, wherein: the cutting device comprises a first cutting assembly for cutting the first adhesive tape on the first applicator roll and a second cutting assembly for cutting the second adhesive tape on the second applicator roll.
7. The intelligent pleurotus eryngii breeding system according to claim 6, wherein: the first shearing assembly comprises a first cutter, a second cutter and a first opening and closing driving device for driving the first cutter and the second cutter to open and close; the second shearing assembly comprises a third cutter, a fourth cutter and a second opening and closing driving device for driving the third cutter and the fourth cutter to open and close.
8. The intelligent pleurotus eryngii breeding system according to claim 7, wherein: the cultivation container comprises a cultivation bottle.
9. The intelligent pleurotus eryngii breeding system according to claim 8, wherein: a first separation groove for the first adhesive tape to pass through and a second separation groove for the second adhesive tape to pass through are formed on the bottleneck of the cultivation bottle.
10. The intelligent pleurotus eryngii breeding system according to claim 9, wherein: in S4, the cultivation bottles are placed in order on a cultivation rack, and the cultivation bottles are transported to a fruiting room through the cultivation rack for fruiting.
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| CN202010910416.2A CN112106653A (en) | 2020-09-02 | 2020-09-02 | Intelligent pleurotus eryngii breeding system and method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201138954Y (en) * | 2007-11-01 | 2008-10-29 | 华富生物科技(上海)有限公司 | Artificial cultivation container for mushroom products |
| CN205794182U (en) * | 2016-07-15 | 2016-12-14 | 甘肃民族师范学院 | Device for culturing pleurotus eryngii |
| CN110946037A (en) * | 2019-12-05 | 2020-04-03 | 江苏香如生物科技股份有限公司 | Pleurotus eryngii factory cultivation and breeding method |
-
2020
- 2020-09-02 CN CN202010910416.2A patent/CN112106653A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201138954Y (en) * | 2007-11-01 | 2008-10-29 | 华富生物科技(上海)有限公司 | Artificial cultivation container for mushroom products |
| CN205794182U (en) * | 2016-07-15 | 2016-12-14 | 甘肃民族师范学院 | Device for culturing pleurotus eryngii |
| CN110946037A (en) * | 2019-12-05 | 2020-04-03 | 江苏香如生物科技股份有限公司 | Pleurotus eryngii factory cultivation and breeding method |
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Application publication date: 20201222 |