CN112105731B - 微小rna表达构建体及其用途 - Google Patents

微小rna表达构建体及其用途 Download PDF

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Publication number
CN112105731B
CN112105731B CN201980030607.5A CN201980030607A CN112105731B CN 112105731 B CN112105731 B CN 112105731B CN 201980030607 A CN201980030607 A CN 201980030607A CN 112105731 B CN112105731 B CN 112105731B
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sequence
expression construct
mirna
mirna expression
cells
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Chinese (zh)
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CN112105731A (zh
Inventor
K-H·克劳斯
F·罗塞特
P·萨蒙
M·埃雷杉德里尼
R·斯佩克
S·布雷德尔
T·梅兰波
R·麦博格
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Universite de Geneve
Zurich Universitaet Institut fuer Medizinische Virologie
Hopitaux Universitaires De Geneve
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Universite de Geneve
Zurich Universitaet Institut fuer Medizinische Virologie
Hopitaux Universitaires De Geneve
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/421—Immunoglobulin superfamily
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/4214—Receptors for cytokines
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201980030607.5A 2018-03-30 2019-04-01 微小rna表达构建体及其用途 Active CN112105731B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862650403P 2018-03-30 2018-03-30
US201862650387P 2018-03-30 2018-03-30
US62/650,403 2018-03-30
US62/650,387 2018-03-30
PCT/IB2019/000328 WO2019186274A2 (en) 2018-03-30 2019-04-01 Micro rna expression constructs and uses thereof

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CN112105731B true CN112105731B (zh) 2024-08-30

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EP (1) EP3775214A2 (https=)
JP (1) JP7607872B2 (https=)
CN (1) CN112105731B (https=)
AU (1) AU2019244479B2 (https=)
CA (1) CA3133899A1 (https=)
SG (1) SG11202009697RA (https=)
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ZA (1) ZA202006505B (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3105816A1 (en) 2018-07-10 2020-01-16 Precigen, Inc. Ror-1 specific chimeric antigen receptors and uses thereof
EP3963076A1 (en) * 2019-05-02 2022-03-09 Celyad Cells with multiplexed inhibitory rna
CN115715295A (zh) 2020-05-27 2023-02-24 安逊生物科学股份有限公司 将car t细胞重新定向到感兴趣的抗原的衔接分子
AU2022205683A1 (en) * 2021-01-11 2023-07-06 Precigen, Inc. Chimeric receptor therapy
GB202106354D0 (en) * 2021-05-04 2021-06-16 Celyad S A Improved chimeric and engineered scaffolds doe multiplexed ingibitory RNA
GB202201927D0 (en) * 2022-02-14 2022-03-30 Kings College Artificial microrna construct
CN114774364B (zh) * 2022-04-26 2024-04-26 深圳市体内生物医药科技有限公司 一种嵌合抗原受体t细胞及其制备方法和应用
GB202206507D0 (en) 2022-05-04 2022-06-15 Antion Biosciences Sa Expression construct
IL322949A (en) 2023-03-03 2025-10-01 Arsenal Biosciences Inc Systems targeting PSMA and CA9
GB202306619D0 (en) 2023-05-04 2023-06-21 Antion Biosciences Sa Cell
GB202402745D0 (en) 2024-02-27 2024-04-10 Antion Biosciences Sa Cell

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5610042A (en) 1991-10-07 1997-03-11 Ciba-Geigy Corporation Methods for stable transformation of wheat
ATE398679T1 (de) 1992-07-07 2008-07-15 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
JP2952041B2 (ja) 1992-07-27 1999-09-20 パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法
GB9222888D0 (en) 1992-10-30 1992-12-16 British Tech Group Tomography
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
CN101818145A (zh) 1998-03-20 2010-09-01 联邦科学和工业研究组织 控制基因表达
EP1071753A2 (en) 1998-04-20 2001-01-31 Ribozyme Pharmaceuticals, Inc. Nucleic acid molecules with novel chemical compositions capable of modulating gene expression
HK1047109A1 (zh) 1999-10-15 2003-02-07 University Of Massachusetts 作为指定基因干预工具的rna干预轨迹基因
US20040019001A1 (en) 2002-02-20 2004-01-29 Mcswiggen James A. RNA interference mediated inhibition of protein typrosine phosphatase-1B (PTP-1B) gene expression using short interfering RNA
DE10224242A1 (de) 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
EP1678315B1 (en) 2003-09-19 2011-08-03 Sangamo BioSciences, Inc. Engineered zinc finger proteins for regulation of gene expression
EP2500427B1 (en) 2007-11-22 2014-07-30 Japan Science and Technology Agency Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
CN101993992A (zh) * 2009-08-13 2011-03-30 薛晓舟 轮毂单元表面的热处理方法
CN101993892A (zh) * 2009-08-31 2011-03-30 长沙赢润生物技术有限公司 肿瘤细胞中靶向性表达shRNA的真核表达载体
US20140199279A1 (en) 2011-01-03 2014-07-17 Bluebird Bio, Inc. Methods for enhancing the delivery of gene-transduced cells
EP2875133B1 (en) * 2012-07-17 2018-01-10 Université de Genève Nucleic acids for down-regulation of gene expression
CN113684185A (zh) 2013-02-26 2021-11-23 纪念斯隆-凯特琳癌症中心 用于免疫疗法的组合物和方法
US20150051267A1 (en) * 2013-08-16 2015-02-19 Purdue Research Foundation BICISTRONIC GENE TRANSFER TOOLS FOR DELIVERY OF miRNAS AND PROTEIN CODING SEQUENCES
CN106061488B (zh) 2013-12-02 2021-04-09 菲奥医药公司 癌症的免疫治疗
US20170335331A1 (en) 2014-10-31 2017-11-23 The Trustees Of The University Of Pennsylvania Altering Gene Expression in CART Cells and Uses Thereof
CN105886473B (zh) * 2015-01-26 2021-05-07 温州医科大学 一种稳定沉默fgfr4基因表达的肝癌细胞株的制备方法
WO2016196388A1 (en) * 2015-05-29 2016-12-08 Juno Therapeutics, Inc. Composition and methods for regulating inhibitory interactions in genetically engineered cells
US12252700B2 (en) 2015-11-13 2025-03-18 Id Pharma Co., Ltd. Paramyxovirus vector
CN105861551B (zh) * 2016-04-21 2019-05-31 南京医科大学 联合表达microRNAs抑制乳腺癌细胞增殖的载体及其构建方法和应用
CN121102467A (zh) 2016-04-22 2025-12-12 克莱格医学有限公司 用于细胞免疫疗法的组合物和方法
EP3464591A4 (en) 2016-05-27 2020-02-19 Shi-Lung Lin A composition and method of using mir-302 precursors as anti-cancer drugs for treating human lung cancer
AU2017292582C1 (en) 2016-07-08 2021-11-11 American Gene Technologies International Inc. HIV pre-immunization and immunotherapy
CN106282212B (zh) * 2016-08-24 2020-03-10 南通大学附属医院 三联人工miRNA抑制VEGFRs制备及其应用
US10457940B2 (en) * 2016-09-22 2019-10-29 University Of Massachusetts AAV treatment of Huntington's disease
CN107312800B (zh) 2017-08-01 2020-02-07 北京舜雷科技有限公司 能敲低内源性pd-1表达的cik及其制备方法与应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Myburgh R等.Optimization of Critical Hairpin Features Allows miRNA-based Gene Knockdown Upon Single-copy Transduction.Mol Ther Nucleic Acids.2014,第3卷(第10期),第1页右栏第1段，第2页左栏第3段，第3页左栏第1段，图2注释，补充图3，补充材料. *
Optimization of Critical Hairpin Features Allows miRNA-based Gene Knockdown Upon Single-copy Transduction;Myburgh R等;Mol Ther Nucleic Acids;第3卷(第10期);第1页右栏第1段，第2页左栏第3段，第3页左栏第1段，图2注释，补充图3，补充材料 *

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ZA202006505B (en) 2024-02-28
EP3775214A2 (en) 2021-02-17
SG11202009697RA (en) 2020-10-29
AU2019244479A1 (en) 2020-11-12
AU2019244479B2 (en) 2024-12-19
JP7607872B2 (ja) 2025-01-06
WO2019186274A3 (en) 2019-12-05
CN112105731A (zh) 2020-12-18
US20210095278A1 (en) 2021-04-01
CA3133899A1 (en) 2019-10-03
JP2021519587A (ja) 2021-08-12
US11649455B2 (en) 2023-05-16
WO2019186274A2 (en) 2019-10-03

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