CN112089637A - Skin care composition with synergistic anti-aging effect and preparation method and application thereof - Google Patents
Skin care composition with synergistic anti-aging effect and preparation method and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61Q19/00—Preparations for care of the skin
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to a skin care composition with a synergistic anti-aging effect, which comprises the following components in parts by weight: about 3 to about 5 parts by weight of gamma-aminobutyric acid; about 1 to 5 parts by weight of Pterocarpus marsupium bark extract; and about 1-20 parts by weight of tomato/yeast fermentation product filtrate. The skin care composition provided by the invention consists of gamma-aminobutyric acid, a Pterocarpus marsupium bark extract and a tomato/yeast fermentation product filtrate, and tests on the anti-aging effect of the single action and the combined use of each component show that the combined use of the three components has stronger effects on reducing the number, depth, length and area of wrinkles and improving the skin elasticity, and the remarkable synergistic effect of the three components on the anti-aging aspect is proved; secondly, tests show that the skin irritation of the group consisting of gamma-aminobutyric acid, Pterocarpus marsupium bark extract and tomato/yeast fermentation product filtrate is low-sensitive, and the combination of the gamma-aminobutyric acid, the Pterocarpus marsupium bark extract and the tomato/yeast fermentation product filtrate overcomes the problem of high irritation caused by the single use of 5% of gamma-aminobutyric acid.
Description
Technical Field
The invention belongs to the technical field of cosmetics. More particularly, relates to a skin care composition with synergistic anti-aging effect, a preparation method and application thereof.
Background
External factors such as ultraviolet rays and expression squeezing easily stimulate the dermis of the skin to excessively secrete neutrophil elastase, so that collagen, elastin and the like in the skin are excessively decomposed to cause deep wrinkles. At present, retinol, vitronectin and the like are mainly adopted in the market to achieve the wrinkle removing effect, but most of the retinol and vitronectin are only effective on light wrinkles of the skin, but the retinol and vitronectin have little effect on improving deep wrinkles. Like the essence of the purple iron of the Eriya group, the effects of promoting the generation of collagen and accelerating the skin regeneration are realized by using vitreous color as a main wrinkle-removing and anti-aging component and compounding high-concentration hyaluronic acid, but the effect on deep wrinkles is not obvious according to the reflection of most subjects.
At present, invasive methods are adopted for achieving the purpose of improving deep wrinkles, for example, injection of hyaluronic acid is used for filling wrinkles, and injection of botulinum toxin is used for paralysis and relaxation of facial expression muscles to achieve the effect of rapid wrinkle removal, but the invasive anti-aging methods are all accompanied by great side effects and risks of infection, and the quality of the effect depends on the level of an injector.
The gamma-aminobutyric acid is a natural amino acid widely distributed in animals and plants, has small molecular weight, can quickly penetrate through skin and directly reach the bottom of the skin, and has the effects of reducing fine wrinkles, smoothing the skin and enabling the skin to be young; meanwhile, the skin barrier can be repaired, and the rough skin can be improved. However, when it is used in an appropriate or recommended amount (5.0%), it tends to cause a large irritation to the skin, and there is a possibility that the skin may suffer from uncomfortable symptoms such as stinging, burning, redness and swelling, and it is confirmed that it has no significant positive effect of reducing the depth of deep wrinkles when it is used alone.
Pterocarpus marsupium bark extract, which is rich in pterostilbene, has excellent antioxidant, whitening, anti-inflammatory and ultraviolet ray resisting effects, is commonly used as a whitening active ingredient, and has no application in anti-aging and wrinkle-removing.
The tomato is rich in vitamins, organic acids, alkaloids and flavonoids, wherein the vitamins comprise carotene, vitamin B1, vitamin A, vitamin C, vitamin D, lycopene, etc. The tomato extract has proliferation effect on epidermal keratinocyte, has activating effect on cathepsin, can enhance skin metabolism, and can be used for preparing antiaging cosmetic in combination with antioxidant effect.
Disclosure of Invention
The invention aims to overcome the defects of the existing anti-aging product and provide an anti-aging preparation which effectively aims at deep wrinkles and has small irritation to skin.
The invention aims to provide a skin care composition with synergistic anti-aging effect, which comprises the following components in percentage by weight:
about 3 to about 5 parts by weight of gamma-aminobutyric acid;
about 1 to 5 parts by weight of Pterocarpus marsupium bark extract; and
about 1-20 parts by weight of tomato/yeast fermentation product filtrate.
Preferably, the skin care composition with synergistic anti-aging effect comprises:
about 5 parts by weight of gamma-aminobutyric acid;
about 5 parts by weight of Pterocarpus marsupium bark extract; and
about 12 parts by weight of tomato/yeast fermentation product filtrate.
Preferably, the skin care composition with synergistic anti-aging effect comprises:
about 5 parts by weight of gamma-aminobutyric acid;
about 5 parts by weight of Pterocarpus marsupium bark extract; and
about 20 parts by weight of tomato/yeast fermentation product filtrate.
Preferably, the specific preparation method of the tomato/yeast fermentation product filtrate comprises the following steps:
s1, taking fresh tomatoes, crushing tissues, filtering out juice, concentrating under reduced pressure to dryness, adding 50-70% acetone solution, leaching for 1-3 h, filtering, and concentrating under reduced pressure to obtain a tomato extract;
s2, adding the tomato extract into the yeast fermentation culture medium, and sterilizing; inoculating saccharomycetes after seed culture according to the inoculation amount of 2-5%, culturing for 2-5 days, filtering, taking fermentation liquor, centrifuging, removing supernatant, and grinding a centrifugate for 0.1-0.5 h;
s3, adding deionized water with the weight 2-5 times that of the ground centrifugate, uniformly mixing, leaching for 1-3 h, and filtering to obtain a tomato/yeast fermentation product filtrate.
Preferably, in the step S2, the yeast is candida utilis.
Preferably, in step S2, the composition of the yeast fermentation medium is: 12-20 g/L glucose, 8-10 g/L peptone and 5-8 g/L, KH ammonium sulfate2P04 2.5g/L、MgSO4.7H2O0.1-0.5 g/L, and pH 5.2-5.6. More preferably, the composition of the yeast fermentation medium is: glucose 20g/L, peptone 8g/L, ammonium sulfate 8g/L, KH2P04 2.0g/L、MgSO4.7H2O0.3 g/L and pH 5.5.
Preferably, in step S2, the fermentation parameters are: the fermentation temperature is 28-32 ℃; the rotating speed is 120-180 r/min; the ventilation amount is 1 to 4 VVM. More preferably, the fermentation temperature is 28 ℃ or 30 ℃; the rotating speed is 160r/min, 150r/min or 120 r/min.
Preferably, in the step S2, 12-23% by volume of the tomato extract is added to the yeast fermentation medium. More preferably, 16% by volume of the tomato extract is added to the yeast fermentation medium.
The invention also aims to provide the application of the skin care composition in preparing cosmetics with anti-aging effect. The anti-aging effect is mainly reflected in the improvement of wrinkles, particularly the improvement of deep wrinkles, wherein the deep wrinkles refer to wide furrows with the depth of between 20 and 100 mu m, and belong to 4-5 grades according to the Lemperle facial wrinkle grading standard. The improvement in deep wrinkles described herein is manifested in a lightening of the wrinkle depth or a reduction in the number of wrinkles.
From the anti-aging test results, the gamma-aminobutyric acid, the Pterocarpus marsupium bark extract and the tomato/yeast fermentation product filtrate have a certain synergistic effect on anti-aging, have a remarkable improvement effect on deep wrinkles with the depth of more than 70 mu m, remarkably reduce the number and depth of wrinkles after use, remarkably increase the skin elasticity, and have an effect far superior to that of the three single-use products.
Another object of the present invention is to provide a cosmetic with synergistic anti-aging effect, which comprises the skin care composition. Preferably, the cosmetic with the synergistic anti-aging effect is used, and the weight of the skin care composition in the cosmetic is 1-99%.
The cosmetic may also include adjuvants common in the cosmetic arts, including, but not limited to: solvent, thickener, emulsifier, humectant, antiseptic, pH regulator, chelating agent and essence.
In some embodiments, the cosmetic of the present invention is a essence, which is composed of the following components in parts by mass: the skin care composition comprises 1-55% of a skin care composition, 3-6% of a humectant, 0.2-0.5% of a thickener, 3-6% of an emulsifier, 0.01-0.02% of a chelating agent, 0.02-0.05% of a preservative and the balance of water.
In some embodiments, the essence consists of the following components in percentage by mass:
the skin care composition comprises, by weight, 1-55% of a skin care composition, 1-3% of glycerin, 1-3% of butanediol, 0.2-0.5% of a polyacrylate cross-linked polymer, 1-3% of sorbitan laurate, 201-3% of polysorbate-1, 0.1-0.3% of hydroxyethyl cellulose, 0.01-0.02% of EDTA-2Na, 0.02-0.05% of phenoxyethanol and the balance of water.
In some embodiments, the essence consists of the following components in percentage by mass:
the skin care composition comprises 22% of skin care composition, 3% of glycerin, 3% of butanediol, 0.35% of polyacrylate cross-linked polymer, 3% of sorbitan laurate, polysorbate-202%, 0.15% of hydroxyethyl cellulose, 0.01% of EDTA-2Na, 0.03% of phenoxyethanol and the balance of water.
The invention has the following beneficial effects:
1) the skin care composition provided by the invention consists of gamma-aminobutyric acid, a Pterocarpus marsupium bark extract and a tomato/yeast fermentation product filtrate, and tests on the anti-aging effect of the single action and the combined use of each component show that the combined use of the three components shows stronger action in the aspects of reducing the number and depth of wrinkles and improving the skin elasticity, so that the three components are proved to have obvious synergistic action in the aspect of anti-aging; tests prove that the skin elasticity is obviously improved after 1 week of use of the skin care composition, and the obvious wrinkle lightening effect can be seen after 2 weeks of use.
2) Tests show that the skin irritation of the gamma-aminobutyric acid, the Pterocarpus marsupium bark extract and the tomato/yeast fermentation product filtrate group is low-sensitive, and the combination of the gamma-aminobutyric acid, the Pterocarpus marsupium bark extract and the tomato/yeast fermentation product filtrate group overcomes the problem of high irritation caused by the single use of 5% of gamma-aminobutyric acid.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
The yeast fermentation medium comprises the following components: glucose 20g/L, peptone 8g/L, ammonium sulfate 8g/L, KH2P04 2.0g/L、MgSO4.7H2O0.3 g/L and pH 5.5.
Example 1 skin care composition
Preparation of tomato/yeast fermentation product filtrate
S1, taking fresh tomatoes, crushing tissues, filtering juice, concentrating under reduced pressure to dryness, adding 70% acetone solution, leaching for 3 hours, filtering, and concentrating under reduced pressure to obtain a tomato extract;
s2, adding tomato extract with the volume of 16% into the yeast fermentation medium, and sterilizing; inoculating 5% of the strain into Candida utilis cultured by seeds, culturing for 4 days under the conditions that the fermentation temperature is 28 ℃, the rotating speed is 160r/min and the ventilation volume is 1-4 VVM, filtering, taking fermentation liquor, centrifuging at 4000r/min, removing supernatant, and grinding a centrifugate for 0.5 h;
s3, adding deionized water with the weight 3 times of that of the ground centrifugate, uniformly mixing, leaching for 3 hours, and filtering to obtain the tomato/yeast fermentation product filtrate.
The skin care composition of this example 1 consisted of 5 parts by weight of gamma-aminobutyric acid, 5 parts by weight of Pterocarpus marsupium bark extract and 12 parts by weight of tomato/yeast fermentation product filtrate.
Example 2 skin care composition
Preparation of tomato/yeast fermentation product filtrate
S1, taking fresh tomatoes, crushing tissues, filtering juice, concentrating under reduced pressure to dryness, adding 50% acetone solution, leaching for 2h, filtering, and concentrating under reduced pressure to obtain a tomato extract;
s2, adding tomato extract with the volume of 20% into the yeast fermentation medium, and sterilizing; inoculating 5% of the strain into Candida utilis cultured by seeds, culturing for 5 days under the conditions that the fermentation temperature is 28 ℃, the rotating speed is 180r/min and the ventilation volume is 1-4 VVM, filtering, taking fermentation liquor, centrifuging at 4000r/min, removing supernatant, and grinding a centrifugate for 0.5 h;
s3, adding deionized water with the weight 5 times of that of the ground centrifugate, uniformly mixing, leaching for 2 hours, and filtering to obtain the tomato/yeast fermentation product filtrate.
The skin care composition described in this example 2 consisted of 5 parts by weight of gamma-aminobutyric acid, 5 parts by weight of Pterocarpus marsupium bark extract and 20 parts by weight of tomato/yeast fermentation product filtrate.
Example 3 skin care composition
Preparation of tomato/yeast fermentation product filtrate
S1, taking fresh tomatoes, crushing tissues, filtering juice, concentrating under reduced pressure to dryness, adding 60% acetone solution, leaching for 3 hours, filtering, and concentrating under reduced pressure to obtain a tomato extract;
s2, adding tomato extract with the volume of 12% into the yeast fermentation culture medium, and sterilizing; inoculating 5% of the strain into Candida utilis cultured by seeds, culturing for 3 days under the conditions that the fermentation temperature is 28 ℃, the rotating speed is 150r/min and the ventilation volume is 1-4 VVM, filtering, taking fermentation liquor, centrifuging at 4000r/min, removing supernatant, and grinding a centrifugate for 0.5 h;
s3, adding deionized water with the weight 5 times of that of the ground centrifugate, uniformly mixing, leaching for 3 hours, and filtering to obtain the tomato/yeast fermentation product filtrate.
The skin care composition of this example 3 consisted of 5 parts by weight of gamma-aminobutyric acid, 3 parts by weight of Pterocarpus marsupium bark extract and 15 parts by weight of tomato/yeast fermentation product filtrate.
Example 4-6 essence with significant anti-aging effect
Note: examples 4-6 skin care compositions the skin care compositions of examples 1-3 were used, respectively.
The preparation method comprises the following steps:
adding deionized water into a water phase pot according to the formula amount, slowly adding polyacrylate cross-linked polymer while stirring, uniformly dispersing, adding butanediol, glycerol and EDTA-2Na, heating to 80 ℃, uniformly stirring, and keeping the temperature for 3min for later use; adding sorbitan laurate, polysorbate-20 and hydroxyethyl cellulose into an oil phase pot according to the formula ratio, heating to 80 ℃, uniformly stirring, and keeping the temperature for 5 min; filtering the materials in the water phase and oil phase, sequentially pumping into a vacuum emulsifying pot, homogenizing for 8min, slowly cooling to 40 deg.C, adding skin care composition and phenoxyethanol, stirring, discharging after detection, and bottling.
Comparative example 1 differs from example 4 in that the skin care composition is gamma aminobutyric acid only, the difference is filled with water, and the remaining parameters are the same as in example 4.
Comparative example 2, which differs from example 4 in that the skin care composition is only a rosewood bark extract, except for the portion filled with water, the remaining parameters are the same as in example 4.
Comparative example 3 differs from example 4 in that the skin care composition is only tomato/yeast fermentation product filtrate, the difference is filled with water, and the remaining parameters are the same as in example 4.
Comparative example 4, which is different from example 4 in that the skin care composition was 5 parts by weight of gamma-aminobutyric acid and 5 parts by weight of Pterocarpus marsupium bark extract, except that the difference was filled with water, and the remaining parameters were the same as example 4.
Comparative example 5 differs from example 4 in that instead of the tomato/yeast fermentation product filtrate, tomato extract was used, with the remaining parameters being the same as in example 4.
The preparation method of the tomato extract comprises the following steps:
pulverizing fresh fructus Lycopersici Esculenti, filtering to obtain juice, concentrating under reduced pressure to dry, adding 70% acetone solution, leaching for 3 hr, filtering, and concentrating under reduced pressure to obtain fructus Lycopersici Esculenti extract.
Test example one, patch test
240 volunteers of 22-50 years old who meet the test requirements are selected as test subjects, the test subjects are randomly divided into 8 groups, 30 individuals in each group are tested, according to skin-enclosed patch tests of human skin patch tests in technical Specification for cosmetic safety, whether the products of examples 4-6 and comparative examples 1-5 have potential possibility of causing adverse reaction to human bodies or not, the results are interpreted according to the interpretation standards of the following Table 1, and the statistical interpretation results are shown in the following Table 2.
TABLE 1 interpretation criteria
TABLE 2 Patch test results
| Sample (I) | Number of negative cases | Number of suspected reaction cases | Number of weak yang cases | Number of strong yang | Number of extreme positive cases |
| Example 4 | 30 | 0 | 0 | 0 | 0 |
| Example 5 | 30 | 0 | 0 | 0 | 0 |
| Example 6 | 30 | 0 | 0 | 0 | 0 |
| Comparative example 1 | 2 | 12 | 14 | 2 | 0 |
| Comparative example 2 | 24 | 6 | 0 | 0 | 0 |
| Comparative example 3 | 28 | 2 | 0 | 0 | 0 |
| Comparative example 4 | 16 | 10 | 4 | 0 | 0 |
| Comparative example 5 | 26 | 4 | 0 | 0 | 0 |
The patch test results show that the skin care composition of examples 4-6 of the invention has low sensitivity, and analysis of test results of other comparative examples shows that when the skin care composition is 5% of gamma-aminobutyric acid (comparative example 1), the skin is greatly stimulated, suspicious reactions occur in 12 cases, and positive reactions occur in 16 cases; when the skin care composition was 5% gamma aminobutyric acid + 5% Pterocarpus marsupium bark extract (comparative example 4), the irritation to the skin was reduced compared to comparative example 1, showing that compounding Pterocarpus marsupium bark extract with gamma aminobutyric acid reduces the irritation; from the results of comparative example 5, it can be seen that the skin care composition obtained by combining the tomato/yeast fermentation product filtrate with γ -aminobutyric acid and Pterocarpus marsupium bark extract has lower irritation than the tomato extract, indicating that the tomato/yeast fermentation product filtrate also has a certain effect of reducing irritation of γ -aminobutyric acid.
Test example two, efficacy test
1.1 subject inclusion criteria: selecting healthy Chinese females with the age of 30-60 years and without serious chronic consumptive diseases; no skin injury, inflammation, etc.; the face has obvious wrinkles with the depth of 70-100 mu m, and the skin is rough and lacks elasticity; facial skin texture was apparent and loose; no participation in other clinical trials during the trial; subjects were able to follow the trial and sign informed consent.
1.2 test methods: 160 subjects were selected according to the selection criteria, and were randomly divided into 8 groups, 20 groups of examples 4 to 6 and comparative examples 1 to 5, respectively, and each group of subjects was cleaned every morning and evening, and then the soybean-sized product was emulsified in the palm, and then absorbed by pressing the face for 28 days.
1.3 index determination:
1.3.1 skin elasticity analysis: the skin elasticity of each group of subjects before and after use on the 7 th, 14 th and 28 th days is tested by a Cutomer (MPA580), the parameter R2 (the ratio of the rebound quantity without negative pressure to the maximum stretching quantity with negative pressure) is taken as the judgment basis of the skin elasticity, the closer the numerical value is to 1, the better the skin elasticity is shown, the same part is measured for 3 times, the average value is taken, and the result is shown in the following table 3;
TABLE 3 results of skin elasticity analysis
| Sample (I) | D0 | D7 | D14 | D28 |
| Example 4 | 0.581±0.023 | 0.645±0.032* | 0.677±0.037** | 0.686±0.042** |
| Example 5 | 0.534±0.018 | 0.582±0.024* | 0.602±0.029** | 0.611±0.030** |
| Example 6 | 0.602±0.035 | 0.659±0.027* | 0.674±0.033** | 0.682±0.017** |
| Comparative example 1 | 0.553±0.012 | 0.576±0.033 | 0.605±0.041* | 0.615±0.038** |
| Comparative example 2 | 0.586±0.025 | 0.589±0.015 | 0.592±0.022 | 0.604±0.030 |
| Comparative example 3 | 0.579±0.043 | 0.583±0.029 | 0.602±0.034 | 0.615±0.026* |
| Comparative example 4 | 0.535±0.011 | 0.552±0.028 | 0.567±0.021* | 0.573±0.036* |
| Comparative example 5 | 0.598±0.028 | 0.612±0.032 | 0.637±0.043* | 0.666±0.032** |
Note: in contrast to the D0, the film,*P＜0.05，**P＜0.01。
the above table shows that the elasticity of the skin can be remarkably improved after 14 days and 28 days of use of the gamma-aminobutyric acid and the tomato/yeast fermentation product filtrate respectively, wherein the elasticity of the gamma-aminobutyric acid at 28 days is very remarkably different (P is less than 0.01) compared with that before use, but the combination of the gamma-aminobutyric acid and the tomato/yeast fermentation product filtrate does not show a synergistic effect; the Pterocarpus marsupium bark extract has no obvious promotion effect on skin elasticity, but good synergistic effect is obtained after the three extracts are used in combination, the skin elasticity is obviously different from that before the extract is used on the 7 th day, and the skin elasticity is very obviously different (P < 0.01) on the 14 th day.
1.3.2 skin wrinkle improvement assay: 3D wrinkle images of the skin before and after use were obtained using Primos lite and its analysis software, wrinkle depth and number were analyzed, the lower the number of wrinkles, the lighter the skin wrinkles, i.e., the better the skin surface condition, the smoother the skin, and the results of the differential analysis were performed using statistical software, as shown in Table 4 below.
Table 4 wrinkle improvement analysis results
Note: in contrast to the D0, the film,*P＜0.05，**P＜0.01。
as can be seen from the above table, after 14 days of using the essence of example 4 of the present invention, the depth and the number of wrinkles are significantly different from those before use, and after 28 days of use, the depth and the number of wrinkles are significantly different from those before use (P < 0.01), which shows that γ -aminobutyric acid, the Pterocarpus marsupium bark extract and the tomato/yeast fermentation product filtrate have synergistic effects in reducing the depth of deep wrinkles and reducing the number of wrinkles, and combined with the patch test results, the Pterocarpus marsupium bark extract and the tomato/yeast fermentation product filtrate can inhibit the allergic reaction caused by γ -aminobutyric acid, and significantly improve the anti-aging and anti-wrinkle effects of γ -aminobutyric acid.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A skin care composition having synergistic anti-aging properties, comprising:
about 3 to about 5 parts by weight of gamma-aminobutyric acid;
about 1 to 5 parts by weight of Pterocarpus marsupium bark extract; and
about 1-20 parts by weight of tomato/yeast fermentation product filtrate.
2. The skin care composition of claim 1, comprising:
about 5 parts by weight of gamma-aminobutyric acid;
about 5 parts by weight of Pterocarpus marsupium bark extract; and
about 12 parts by weight of tomato/yeast fermentation product filtrate.
3. The skin care composition of claim 1, comprising:
about 5 parts by weight of gamma-aminobutyric acid;
about 5 parts by weight of Pterocarpus marsupium bark extract; and
about 20 parts by weight of tomato/yeast fermentation product filtrate.
4. The skin care composition of claim 1, wherein the tomato/yeast fermentation product filtrate is prepared by a method comprising:
s1, taking fresh tomatoes, crushing tissues, filtering out juice, concentrating under reduced pressure to dryness, adding 50-70% acetone solution, leaching for 1-3 h, filtering, and concentrating under reduced pressure to obtain a tomato extract;
s2, adding the tomato extract into the yeast fermentation culture medium, and sterilizing; inoculating saccharomycetes after seed culture according to the inoculation amount of 2-5%, culturing for 2-5 days, filtering, taking fermentation liquor, centrifuging, removing supernatant, and grinding a centrifugate for 0.1-0.5 h;
s3, adding deionized water with the weight 2-5 times that of the ground centrifugate, uniformly mixing, leaching for 1-3 h, and filtering to obtain a tomato/yeast fermentation product filtrate.
5. The skin care composition of claim 4, wherein in step S2, the yeast is Candida utilis.
6. The skin care composition of claim 5, wherein in step S2, the yeast fermentation medium comprises: 12-20 g/L glucose, 8-10 g/L peptone and 5-8 g/L, KH ammonium sulfate2P04 2.5g/L、MgSO4.7H2O0.1-0.5 g/L, and pH 5.2-5.6.
7. The skin care composition according to any one of claims 4 to 6, wherein in step S2, the fermentation parameters are: the fermentation temperature is 28-32 ℃; the rotating speed is 120-180 r/min; the ventilation amount is 1 to 4 VVM.
8. The skin care composition of claim 4, wherein in step S2, 12-23% by volume of the tomato extract is added to the yeast fermentation medium.
9. Use of the skin care composition according to any one of claims 1 to 8 for the preparation of a cosmetic having anti-aging effect.
10. A cosmetic with synergistic anti-aging effect, characterized in that it comprises a skin care composition according to any one of claims 1 to 8.
Priority Applications (1)
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| CN114317628A (en) * | 2021-12-28 | 2022-04-12 | 华熙生物科技股份有限公司 | Low-irritation gamma-aminobutyric acid product, preparation method thereof and application of reducing irritation |
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| WO2009046116A1 (en) * | 2007-10-01 | 2009-04-09 | Dennis Gross | Skin care products containing multiple enhancers |
| CN103127045A (en) * | 2013-02-05 | 2013-06-05 | 新疆优康来生物科技开发有限公司 | Composition containing gamma-aminobutyric acid and lycopene and preparation method thereof |
| CN110731924A (en) * | 2019-07-30 | 2020-01-31 | 浙江养生堂天然药物研究所有限公司 | Enhanced anti-aging cosmetic compositions |
| CN110917109A (en) * | 2018-09-20 | 2020-03-27 | 天津贝罗尼生物科技有限公司 | Anti-aging mask |
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| WO2009046116A1 (en) * | 2007-10-01 | 2009-04-09 | Dennis Gross | Skin care products containing multiple enhancers |
| CN103127045A (en) * | 2013-02-05 | 2013-06-05 | 新疆优康来生物科技开发有限公司 | Composition containing gamma-aminobutyric acid and lycopene and preparation method thereof |
| CN110917109A (en) * | 2018-09-20 | 2020-03-27 | 天津贝罗尼生物科技有限公司 | Anti-aging mask |
| CN110731924A (en) * | 2019-07-30 | 2020-01-31 | 浙江养生堂天然药物研究所有限公司 | Enhanced anti-aging cosmetic compositions |
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| CN114317628A (en) * | 2021-12-28 | 2022-04-12 | 华熙生物科技股份有限公司 | Low-irritation gamma-aminobutyric acid product, preparation method thereof and application of reducing irritation |
| CN114317628B (en) * | 2021-12-28 | 2024-03-08 | 华熙生物科技股份有限公司 | Gamma-aminobutyric acid product with low irritation, preparation method thereof and application of gamma-aminobutyric acid product with low irritation |
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