CN112076141A

CN112076141A - Plant combined enzyme for removing mites and acnes as well as preparation method and application thereof

Info

Publication number: CN112076141A
Application number: CN202011088874.9A
Authority: CN
Inventors: 单承莺
Original assignee: Dongping Jiuxin Biochemical Co ltd; NANJING INSTITUTE FOR COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
Current assignee: Dongping Jiuxin Biochemical Co ltd; NANJING INSTITUTE FOR COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
Priority date: 2020-10-12
Filing date: 2020-10-12
Publication date: 2020-12-15
Also published as: CN113143835A; CN113143835B

Abstract

The invention discloses a plant combined enzyme for removing mites and acnes, which is prepared by carrying out water extraction and fermentation on at least one of schizonepeta, bamboo, celery seeds and orange peels. The preparation method is simple and easy to operate, and can completely extract the effective parts of the raw materials. The result of an in-vitro mite removing experiment on the plant combined enzyme disclosed by the invention shows that the plant combined enzyme has an excellent mite removing effect; and the product has no irritation to human skin, can be directly used, can also be applied to a daily chemical product formula, and can effectively remove mites and acnes.

Description

Plant combined enzyme for removing mites and acnes as well as preparation method and application thereof

Technical Field

The invention relates to a plant combined enzyme for removing mites and acnes, and a preparation method and application thereof.

Background

Acne, commonly known as "whelk" or "comedo", is a chronic inflammatory disease of the sebaceous glands of the hair follicle, which occurs in young people of 15-25 years old, and is called as "whelk". It is clinically characterized by the appearance of acne, papules, pustules, etc., often accompanied by seborrhea; acne removal is a process of treating and eliminating acne by applying medical technology or method.

Recent studies have shown that mites are closely related to acne. The mites parasitic on human bodies are generally divided into two types, namely hair follicle mites and sebaceous gland mites, generally, the probability of the mites infecting the head and face skin is high, the excrement of the mites can cause pore blockage to form black heads, and the skin becomes large in pores and even inflamed after a long time, so that a series of pathological changes such as acne, acne rosacea, folliculitis and the like are caused, and the health of human bodies and the beauty of the face are seriously affected. Therefore, in the process of removing acne, the mite removal is particularly important.

In the current commonly used ocular acarus killing agents, tea tree essential oil, nitazoxanol cream, benzyl benzoate emulsion, permethrin cream, sulfur ointment and the like are externally used; the oral administration medicines comprise metronidazole, ivermectin and the like, but the treatment medicines have various problems, such as strong irritation, easy allergy (such as tea tree essential oil), easy generation of drug resistance (such as ivermectin) of antibiotic medicines and high price, so that the development of a novel mite removing agent has important significance.

Ferment is a generic term for all enzymes involved in various physiological and biochemical activities in the body. At present, ferment is also a general name of products obtained by fermenting various raw materials. The ferment product is prepared from food materials such as fruits and vegetables by adding sugar, sealing and storing, and is fermented completely to obtain fermented liquid. In the process of preparing the ferment, the microorganism carried on the surface of the raw material can be relied on, and the zymophyte can be added artificially; these microbial strains are propagated to form a certain colony structure in the preparation process, thereby completing the whole fermentation process. Since the fermentation process is a complex physiological and metabolic process of microorganisms, the types of substances produced by the fermentation process are also varied. One very important aspect for determining the functions of the ferment is whether the ferment has scientific main material selection and fermentation strain formula, and the fermented product can play a plurality of benefits of beautifying, regulating body functions and the like on a human body under certain conditions.

Disclosure of Invention

The invention aims to solve the problem that an innovative scheme is provided aiming at the defects in the prior art, in particular to a scheme capable of effectively removing mites and acnes.

In order to solve the problems, the invention adopts the following scheme: the mite and acne removing plant combined enzyme is characterized by being prepared by carrying out water extraction and fermentation on at least one of schizonepeta, bamboo, celery seeds and orange peels.

Further, according to the above design scheme, the mite-removing and acne-removing plant combined enzyme is characterized in that the mass component ratio of the schizonepeta tenuifolia, the bamboo, the celery seed and the orange peel is 30-60 parts of the schizonepeta tenuifolia: 20-40 parts of bamboo: 10-30 parts of celery seeds: 10-30 parts of orange peel; the herba schizonepetae is at least one of stems, leaves and flowers of the herba schizonepetae; the bamboo is at least one of green bamboo, tabasheer and bamboo leaf.

Further, the mite-removing and acne-removing plant combined enzyme is characterized in that the mass component ratio of the nepeta cataria, the bamboos, the celery seeds and the orange peels is 40 parts: 30 parts of: 15 parts of: 15 parts.

Further, the mite-removing and acne-removing plant combined enzyme is characterized in that the mass component ratio of the nepeta cataria, the bamboos, the celery seeds and the orange peels is 60 parts: 20 parts of: 10 parts of: 10 parts.

Further, the mite-removing and acne-removing plant combined enzyme is characterized in that the mass component ratio of the nepeta cataria, the bamboos, the celery seeds and the orange peels is 30 parts: 30 parts of: 20 parts of: and 20 parts.

Further, the preparation method of the mite and acne removing plant combined enzyme according to the design scheme is characterized by comprising the following steps: a. mixing herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris, and adding 60% ethanol solution deionized water 30 times of the total mass of the mixture; b. performing ultrasonic oscillation with ultrasonic power of 200W, temperature of the mixed solution of 50 ℃ and ultrasonic oscillation time of 120 min; c. after ultrasonic oscillation is finished, transferring all materials into a water bath heating device, and heating in a water bath until the system keeps a micro-boiling state for 4 hours for continuous extraction; d. after extraction, standing, cooling to 25 ℃, adding sucrose accounting for 5% of the mass of the extracting solution, and uniformly mixing; e. then adding biological compound strains with the mass proportion of 5 percent of the extract to start fermentation, maintaining the temperature at 25 ℃ in a dark condition, sealing and fermenting for 15d, centrifuging the mixed fermentation liquor after the fermentation process is finished, and taking supernatant to be stored at 4 ℃; the plant combined enzyme for removing mites and acnes is prepared.

Further, according to the above design scheme, the preparation method of the plant combined enzyme for removing mites and acnes is characterized in that the bio-compatible strain is a combination of any four to six of nocardia actinomyces (Nocard's Bacillus), Bacillus mucilaginosus (Bacillus mucosus), Bacillus subtilis (Bacillus subtilis), Beauveria bassiana (Beauveria bassiana), Trichoderma reesei (Trichoderma reesei), and Candida albicans (Candida famata).

Further, the mite and acne removing plant combined enzyme is applied to preparation of daily chemical products according to the design scheme.

Further, the mite-removing and acne-removing plant combined enzyme is applied to preparation of daily chemical products, and is characterized in that the daily chemical products comprise water aqua, essence, emulsion, cream, gel, powder, facial mask, handmade soap, spray and washing and protecting products.

Further, the application of the plant combination enzyme for removing mites and removing acnes in the preparation of daily chemical products is characterized in that the addition amount of the plant combination enzyme for removing mites and removing acnes in the daily chemical products is 0.5-50% by mass

The invention has the following technical effects: in the plant combined enzyme extraction raw materials provided by the invention, the Nepeta cataria (Nepeta cataria) is an annual herbaceous plant, is also called catnip and is also called Chenopodium ambrosioides, is mainly medicinal, can be eaten as vegetables when being young and tender, and is widely distributed in China. Volatile essential oil can be extracted from fresh plants of the nepeta cataria and has been reported to have stronger insect repelling effect, and ethanol extracts of the nepeta cataria contain nepetalactone, nepetaic acid and the like; however, no other activity of the extract of nepeta cataria was reported.

Bamboo, also known as Bamboo, the academic name Bambusoideae (bambusacea or bambooo); perennial gramineae, bamboo subfamily, are various in variety, including moso bamboo, dendrocalamus latiflorus, arrowed bamboo, etc. The bamboo skin of the outward bamboo is green when fresh and is called as the green bamboo, and the side of the bamboo cavity with yellow color is called as the tabasheer; the parts of the bamboo applied by the invention are fresh bamboo leaves, green bamboos and yellow bamboos. Bamboo extract has been reported to be applied in cosmetics because of containing flavonoid, phenolic acid, various amino acids and other active ingredients, but no report on the application of the cosmetics for removing mites and pox is found.

Celery belongs to an umbelliferae plant, has a scientific name of Apium grauens L.var.dulctedc, is various, is one of the vegetables which are usually eaten by Chinese people, and also has the effects of calming the liver, clearing heat, dispelling wind, promoting diuresis, relieving restlessness, reducing swelling and the like. Celery seeds, which are mature seeds of celery, have small body forms, are oval, are mostly brown or dark brown, and contain eucalyptol, umbelliferone, apigenin, linolenic acid, volatile oil and various minerals. Celery seeds are considered to have the effects of dispersing qi, reducing swelling, promoting urination, clearing retardation, reducing blood pressure and the like in Chinese traditional medicine.

Orange peel, the fruit peel of Citrus sinensis (L.) Osbeck of Rutaceae; the pericarpium Citri Junoris has effects of regulating qi-flowing, eliminating phlegm, invigorating stomach, and removing food stagnation, and the pericarpium Citri Junoris extract contains hesperidin, limonene etc. as active ingredients, can balance pH of skin, and has certain effect on skin growth and repair.

The invention utilizes the principle of 'monarch, minister, assistant and guide' of Chinese traditional medicine to carry out composition, wherein the herba schizonepetae is monarch drug and has excellent mite-removing and antibacterial effects, and the bamboo is minister drug and has good anti-inflammatory and antibacterial effects; the celery seeds are adjuvant drugs and have the effects of clearing heat, dissipating qi and eliminating stagnation; the pericarpium Citri Junoris is used as a messenger drug, and has effects of harmonizing the drugs, moisturizing, whitening, resisting allergy, etc., improving skin pH value of acne part, and promoting skin repair. After the 4 plant raw materials with various effects are compounded, the biological fermentation technology is utilized to enable the plant raw materials to exert synergistic effect; the fermentation product has special effects of removing acarid, inhibiting bacteria, clearing heat, resisting inflammation, relieving swelling and itching, etc.; the preparation method is simple and convenient, and the obtained product has high content of effective substances. The plant combined enzyme is detected for physiological activity, and is found to have remarkable effects of removing mites and acnes.

The invention discloses a plant combined enzyme with mite and acne removing effects and a preparation method and application thereof. The plant composite enzyme is prepared from the following plant components in parts by weight: 30-60 parts of herba schizonepetae (including stems, leaves and flowers), 20-40 parts of bamboos (including green bamboos, tabasheers and bamboo leaves), 10-30 parts of celery seeds and 10-30 parts of orange peels; the invention takes the 'monarch, minister, assistant and guide' theory in the traditional Chinese medicine as guidance to carry out composition, and utilizes the advanced biological fermentation technology to achieve the aim of removing mites and acnes through the synergistic action of various natural plants. The preparation method comprises two processes of water extraction and fermentation, wherein the water extraction process adopts an ultrasonic-assisted extraction process, and comprises the following steps: crushing the plant components into particles, mixing the particles according to a ratio, adding deionized water, wherein the material-liquid ratio is 1: 10-30, the ultrasonic power is 200-300W, the temperature is 50-70 ℃, and the ultrasonic oscillation extraction is carried out for 60-120 min; then transferring the mixture into a water bath heating device, heating the mixture in a water bath until the system keeps a slightly boiling state, and extracting for 2-4 h; standing after the water extraction process is finished, and adding cane sugar which accounts for 5-15% of the mass of the extracting solution to be uniformly mixed when the extracting solution is cooled to 25-35 ℃; and then adding biological compound strains with the mass ratio of 5% of the extract to start fermentation, and maintaining the temperature at 25-35 ℃ under the condition of keeping out of the sun, and sealing and fermenting for 7-15 days. After the fermentation process is finished, centrifuging the mixed fermentation liquor, and taking the supernatant to store at 4 ℃; namely the plant composite ferment of the invention. The preparation method is simple and easy to operate, and can completely extract the effective parts of the raw materials. The result of an in-vitro mite removing experiment on the plant combined enzyme disclosed by the invention shows that the plant combined enzyme has an excellent mite removing effect; and the product has no irritation to human skin, can be directly used, can also be applied to a daily chemical product formula, and can effectively remove mites and acnes.

Drawings

FIG. 1 is a bar graph of the mortality rate of aqueous extracts of plant combination ferments and plant combinations (not fermented) to mites.

FIG. 2 is a bar graph of mite mortality for different concentrations of plant combination enzymes.

Detailed Description

The process of the present invention is illustrated below by way of specific examples, but the present invention is not limited thereto. The test methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials used, unless otherwise specified, were all commercially available from conventional sources.

Example 1 preparation of plant combination enzyme

Mixing herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris, wherein the mixture accounts for 40g, 30g, 15g, and 15g of herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris in 100g of the mixture respectively; 2kg of deionized water was added; then ultrasonic oscillation is carried out, the ultrasonic power is 250W, the temperature is 60 ℃, and the ultrasonic oscillation time is 90 min; after ultrasonic oscillation is finished, transferring all the materials into a water bath heating device, heating in a water bath until the system keeps a slightly boiling state, continuously extracting for 3 hours, standing after extraction is finished, and cooling to 30 ℃; adding cane sugar with the mass ratio of 10% of the extracting solution and uniformly mixing; then adding biological compound strains with the mass ratio of 5 percent of the extract to start fermentation, maintaining the temperature at 30 ℃ under the condition of keeping out of the sun, and sealing and fermenting for 10 days. After the fermentation process is finished, centrifuging the mixed fermentation liquor, and taking the supernatant to store at 4 ℃; namely the plant composite ferment of the invention. The color of the plant composite enzyme is yellow and semitransparent, and the dry matter content of the plant composite enzyme is determined to be 0.52%.

Example 2 preparation of plant combination enzymes

Mixing herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris, wherein the mixture accounts for 60g, 20g, 10g, and 10g of 100g of herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris; 1kg of deionized water was added; then ultrasonic oscillation is carried out, the ultrasonic power is 300W, the temperature is 70 ℃, and the ultrasonic oscillation time is 60 min; after ultrasonic oscillation is finished, transferring all materials into a water bath heating device, heating in a water bath until the system keeps a slightly boiling state, continuously extracting for 2 hours, standing after extraction is finished, and cooling to 35 ℃; adding sucrose accounting for 15 percent of the mass of the extracting solution and mixing evenly; then adding biological compound strains with the mass ratio of 5 percent of the extract to start fermentation, and maintaining the temperature at 35 ℃ under the condition of keeping out of the sun and carrying out sealed fermentation for 7 days. After the fermentation process is finished, centrifuging the mixed fermentation liquor, and taking the supernatant to store at 4 ℃; namely the plant composite ferment of the invention. The color of the plant composite enzyme is yellow and semitransparent, and the dry matter content of the plant composite enzyme is determined to be 0.94%.

Example 3 preparation of plant combination enzymes

Mixing herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris, wherein the mixture accounts for 30g, 20g, and 20g of herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris in 100g of the mixture respectively; 3kg of deionized water was added; then ultrasonic oscillation is carried out, the ultrasonic power is 200W, the temperature is 50 ℃, and the ultrasonic oscillation time is 120 min; after ultrasonic oscillation is finished, transferring all the materials into a water bath heating device, heating in a water bath until the system keeps a slightly boiling state, continuously extracting for 4 hours, standing after extraction is finished, and cooling to 25 ℃; adding sucrose accounting for 5 percent of the mass of the extracting solution and mixing evenly; then adding biological compound strains with the mass ratio of 5 percent of the extract to start fermentation, maintaining the temperature at 25 ℃ under the condition of keeping out of the sun, and sealing and fermenting for 15 days. After the fermentation process is finished, centrifuging the mixed fermentation liquor, and taking the supernatant to store at 4 ℃; namely the plant composite ferment of the invention. The color of the plant composite enzyme is yellow and transparent, and the dry matter content is measured to be 0.36 percent.

Comparative examples 1 to 3 preparation of plant Combined Water extract

According to the preparation method described in examples 1-3, after the water extraction process, the biofermentation is not performed, the centrifugation is performed, and the extract is collected by filtration, thus obtaining the plant combined water extract corresponding to comparative examples 1-3 respectively.

Example 4 external mite-killing experiment of plant combination enzyme and plant combination aqueous extract liquid

1 materials and methods

1.1 Experimental materials

Equipment: a climatic chamber (Sanyo, MLR-350H, Japan), a stereomicroscope (Olympus, SZ2, Japan), a transparent adhesive tape, and the like.

Experimental mites: the method for obtaining the human demodex by adopting a transparent adhesive tape pasting overnight method comprises the following specific steps: the examinee is ordered to clean the face thoroughly before sleeping, and then the transparent adhesive tapes with the length of 6cm and the width of 1.5cm are respectively stuck to the forehead, the cheek, the nose, the chin and the like of the examinee. And taking off the film the next day, adhering the film on a glass slide, and performing microscopic examination.

Preparing a sample to be tested: distilling the plant combination enzyme and the plant combination water extract prepared in the example 1 and the comparative example 1 under reduced pressure and concentrating to 1/20 of original volume for later use, wherein the samples are No. 1 and No. 2; distilling the plant combination enzyme and the plant combination water extract prepared in the example 2 and the comparative example 2 under reduced pressure and concentrating to 1/10 of the original volume for later use, samples No. 3 and 4; the plant combination enzyme and the plant combination water extract prepared in example 3 and comparative example 3 were distilled under reduced pressure and concentrated to 1/30, the original volume for use, samples No. 5 and No. 6.

1.2 Experimental methods

1.2.1 experimental groups: the number of the liquid to be detected is 1-6, and meanwhile, a 2% metronidazole solution is used as a positive control group, and liquid paraffin is used as a blank control group. Each group of 10 demodex mites tested. The experiment was repeated 3 times for each concentration of the drug solution and the results averaged.

1.2.2 methods of observation: sucking 200 μ L of the liquid medicine with a micropipette, dropping onto a carrier, spreading the liquid medicine with a push sheet, and sticking the adhesive tape for detecting Demodex mites on the glass slide to ensure that the liquid medicine is fully contacted with the worm body. Then the glass slide is placed in an artificial climate box (the temperature is 30 ℃, the humidity is 75 percent), and the death condition of the demodex is observed under a microscope after 4 hours.

1.2.3 criteria for mortality: continuously observing under 400 times microscope for 1min, determining death of mite with proclarm or immobile leg or paw as initial judgment, and determining death of mite with continuous observation after 30 min.

Corrected mortality rate/% (number of live mites in control group-number of live mites in treatment group)/number of live mites in control group × 100

2 results

TABLE 1 corrected mortality of mites by plant combination enzymes and plant single essential oils

Numbering	Corrected mortality (%) + -SE
		No. 1: combined enzyme	68.32±4.15
No. 2: combined water extract	37.16±5.26
		No. 3: combined enzyme	61.08±3.35
No. 4: combined water extract	31.48±3.66
		No. 5: combined enzyme	63.22±5.24
No. 6: combined water extract	35.23±4.065
		Metronidazole group (2%)	56.73±4.07

As can be seen from the results of Table 1 and FIG. 1, the plant combined enzyme has a remarkable killing effect on human demodex, the lethality is 60-70%, and is remarkably higher than that of a positive control group by 2% metronidazole; the plant combined water extract without biological fermentation also has a certain killing effect on human demodex, and the fatality rate is 30-40% and is lower than that of a positive control group. Therefore, the biological fermentation process promotes further dissolution and transformation of effective substances in the plant formula, and the acaricidal effect is obviously improved.

Example 5 inhibition of tyrosinase Activity by plant combination enzyme and plant combination aqueous extract

Tyrosinase (Tyrosinase, TYR), also known as polyphenol oxidase, participates in the first two steps of melanin synthesis, and the activity of Tyrosinase is related to the amount of melanin synthesis. In human body, tyrosinase is mainly present in melanocytes of epidermal cells of skin, and has an important relationship with the occurrence of some common skin diseases such as dyschromatosis and malignant melanoma. Therefore, the tyrosinase inhibitor is also used as an additive with the effects of whitening and lightening spots and added into cosmetics to improve the tyrosinase metabolism of pigment cells in skin and prevent pigmentation, so that the problem of the acne spots after healing of the acne spots can be improved, and lightening of the acne spots is promoted.

1 materials and methods

1.1 Experimental materials

Preparation of 1.1.1L-tyrosine solution (7.5mmol/L)

0.2721g of L-tyrosine is accurately weighed, a plurality of drops of concentrated hydrochloric acid are firstly added, about 50mL of deionized water is added, after the concentrated hydrochloric acid and the deionized water are completely dissolved by slight heating, the pH value is adjusted to 7.0 by using sodium hydroxide solution, and the deionized water is added to a constant volume of 200 mL.

1.1.2 preparation of tyrosinase liquid

Preparing tyrosinase liquid from fresh and intact potatoes, which comprises the following specific operations: cleaning potato, and precooling at 4 deg.C for about 4 h. Peeled, cut into pieces of about 1X 1cm3, and frozen at-20 ℃ overnight. Weighing, adding 4 deg.C pre-cooled sodium phosphate buffer solution at a ratio of 1: 1(W: V), homogenizing with tissue triturator, filtering with 3 layers of gauze, centrifuging the filtrate at 4000r/min for 10min to obtain supernatant as obtained tyrosinase crude enzyme solution, storing at 4 deg.C, and using up in 2 hr.

1.1.3 preparation of test solutions

Taking 1mL of the plant combination enzyme and the plant combination water extract prepared in the example 1 and the comparative example 1 respectively, and diluting to 3mL for later use, wherein the samples are No. 1 and No. 2; taking 1mL of the plant combination enzyme and the plant combination water extract prepared in the example 2 and the comparative example 2 respectively, and diluting to 6mL for later use, wherein the samples are No. 3 and No. 4; the plant combination enzyme and the plant combination water extract prepared in example 3 and comparative example 3 were each 1mL diluted to 2mL for use, samples nos. 5 and 6. The positive control is a 1% arbutin solution.

1.2 Experimental methods

The total reaction system was 5 mL. The specific design is shown in table 2:

TABLE 15 mL test System design

Note: when the absorbance is measured by a spectrophotometer, the test solution, the standard control and the positive control are respectively zeroed by the negative control 1, the negative control 2 and the negative control 3.

In the experiment, phosphate buffer solution, test solution (including positive control) and enzyme solution are sequentially added into a test tube, and water bath is carried out at 30 ℃ for 10 min. The substrate L-tyrosine was then added and the timer was started immediately. The absorbance at a wavelength of 475nm at 20min of the reaction was determined. The inhibition rate of the test solution (including the positive control) on the tyrosinase is calculated by using the following formula with the corresponding negative control as a reference during the determination.

Wherein "A" is the absorbance of the standard control and "B" is the absorbance of the test solution (or positive control).

Each experiment was done in 3 replicates. The high inhibition rate indicates that the inhibition intensity of the compound on tyrosinase activity is high.

2 results

See table 2.

TABLE 2 inhibition ratio of plant enzyme and plant water extract to tyrosinase

Numbering	Inhibition ratio (%)
		No. 1: combined enzyme	86.18±3.17
No. 2: combined water extract	44.13±2.51
		No. 3: combined enzyme	72.36±4.65
No. 4: combined water extract	39.08±3.24
		No. 5: combined enzyme	77.33±3.48
No. 6: combined water extract	41.82±2.90
		Arbutin (0.5%)	46.33±3.76

The results in table 2 and fig. 2 show that: the fermented liquid obtained by fermenting the plant combined water extract, namely the plant combined enzyme prepared in the embodiments 1-3, has the inhibition capability on tyrosinase, which is greatly improved compared with that of the unfermented water extract, and is also greatly superior to that of arbutin with the concentration of 1% of a positive control (in whitening daily chemical products, the common addition concentration of arbutin is 1%). The biological fermentation process promotes further dissolution and transformation of effective substances in the plant formula, and the application value of the plant combined enzyme can be greatly improved.

Example 6 preparation of a mite and acne removing skin care lotion containing phytolacca de-acne phytolacca composition enzyme as a main functional component a formula of the mite and acne removing skin care lotion containing the phytolacca de-acne phytolacca composition enzyme is shown in table 3.

TABLE 3 formula of skin care lotion for removing mites and acne

The preparation process comprises the following steps: heating cetostearyl alcohol, squalane, caprylic capric triglyceride, white oil, and stearyl alcohol polyoxyethylene 2 ether, stirring to 75 deg.C to dissolve completely to obtain phase A; adding appropriate amount of deionized water into plant composite enzyme, Hamamelis mollis extractive solution and carbomer U20, heating and stirring to 75 deg.C for dissolving completely, and keeping the temperature for 20-30 min; then adding glycerol, propylene glycol, butanediol, vitamin E and essence, stirring and dissolving uniformly under the condition of heat preservation, and complementing the balance of deionized water to obtain phase B; adding phase A into phase B, stirring under continuously maintaining temperature, dissolving, cooling to about 45 deg.C, adding TEA and phenoxyethanol, stirring, standing, and cooling to obtain the final product.

The mite-removing acne-removing skin-care emulsion is represented by an embodiment of the plant combined enzyme prepared by the invention in a mite-removing acne-removing daily chemical product, and can also be prepared into daily chemical products with mite-removing and acne-removing effects, such as water aqua, gel, cream, powder, facial mask, manual soap, spray, washing and care products and the like according to a common formula in the daily chemical industry.

Example 7 evaluation of Effect of mite-removing, acne-removing and skin-caring emulsion

Evaluation target: 44 young people with acne on face and 15-30 years of age were selected, 24 male and 20 female.

The evaluation method comprises the following steps: the mite-removing and acne-removing skin care lotion prepared in example 6 is smeared three times in the morning, noon and evening by a double-blind method, and 28 days are a treatment course.

Evaluation criteria: the effect is remarkable: acne is faded by more than 70 percent; ② has certain effect: acne is faded by more than 30 percent; ③ invalid: the acne is faded by less than 30 percent.

Evaluation results were as follows: 29 cases of obvious effect account for 65.9 percent; 10 effective cases account for 22.7 percent; and 5 invalid cases account for 11.4 percent. The total effective rate reaches 88.6 percent, and no adverse reaction such as skin allergy, red swelling and the like is reported. The mite and acne removing plant combined enzyme has a good acne removing effect.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The mite and acne removing plant combined enzyme is characterized by being prepared by carrying out water extraction and fermentation on at least one of schizonepeta, bamboo, celery seeds and orange peels.

2. The mite-removing and acne-removing plant combined enzyme according to claim 1, wherein 30-60 parts of herba pseudoschizonepetae, bamboo, celery seeds and orange peels are taken according to the mass component ratio: 20-40 parts of bamboo: 10-30 parts of celery seeds: 10-30 parts of orange peel; the herba schizonepetae is at least one of stems, leaves and flowers of the herba schizonepetae; the bamboo is at least one of green bamboo, tabasheer and bamboo leaf.

3. The mite-removing and acne-removing plant combined enzyme according to claim 2, wherein the mass component ratio of the nepeta cataria, the bamboo, the celery seeds and the orange peels is 40 parts: 30 parts of: 15 parts of: 15 parts.

4. The mite-removing and acne-removing plant combined enzyme according to claim 2, wherein the mass component ratio of the nepeta cataria, the bamboo, the celery seeds and the orange peels is 60 parts: 20 parts of: 10 parts of: 10 parts.

5. The mite-removing and acne-removing plant combined enzyme according to claim 2, wherein the mass component ratio of the nepeta cataria, the bamboo, the celery seeds and the orange peels is 30 parts: 30 parts of: 20 parts of: and 20 parts.

6. The preparation method of the plant combined ferment for removing mites and acnes as claimed in claim 1, which comprises the following steps: a. mixing herba Schizonepetae, bamboo, semen Apii Graveolentis, and pericarpium Citri Junoris, and adding 60% ethanol solution deionized water 30 times of the total mass of the mixture; b. performing ultrasonic oscillation with ultrasonic power of 200W, temperature of the mixed solution of 50 ℃ and ultrasonic oscillation time of 120 min; c. after ultrasonic oscillation is finished, transferring all materials into a water bath heating device, and heating in a water bath until the system keeps a micro-boiling state for 4 hours for continuous extraction; d. after extraction, standing, cooling to 25 ℃, adding sucrose accounting for 5% of the mass of the extracting solution, and uniformly mixing; e. then adding biological compound strains with the mass proportion of 5 percent of the extract to start fermentation, maintaining the temperature at 25 ℃ in a dark condition, sealing and fermenting for 15d, centrifuging the mixed fermentation liquor after the fermentation process is finished, and taking supernatant to be stored at 4 ℃; the plant combined enzyme for removing mites and acnes is prepared.

7. The plant-combined ferment for removing mites and acnes as claimed in claim 1, wherein the bio-coincidence bacterial species is a combination of any four to six species selected from the group consisting of Nocardia actinomycetes, Bacillus mucilaginosus, Bacillus subtilis, Beauveria bassiana, Trichoderma reesei and Candida.

8. The application of the mite and acne removing plant combined enzyme according to claim 1 in preparation of daily chemical products.

9. The application of the mite and acne removing plant combined enzyme in preparing daily chemical products according to claim 8, wherein the daily chemical products comprise water aqua, essence, emulsion, cream, gel, powder, facial mask, handmade soap, spray and wash care products.

10. The application of the plant combination enzyme for removing mites and removing acnes in the preparation of daily chemical products according to claim 8, wherein the addition amount of the plant combination enzyme for removing mites and removing acnes in the daily chemical products is 0.5-50% by mass.