CN112068249A - Fiber optical tweezers and system based on special optical fiber - Google Patents
Fiber optical tweezers and system based on special optical fiber Download PDFInfo
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- 239000000835 fiber Substances 0.000 title claims abstract description 104
- 239000013307 optical fiber Substances 0.000 title claims abstract description 57
- 238000012576 optical tweezer Methods 0.000 title claims abstract description 37
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- 238000000034 method Methods 0.000 claims abstract description 7
- 238000012545 processing Methods 0.000 claims description 5
- 238000005530 etching Methods 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 10
- 238000000651 laser trapping Methods 0.000 description 9
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- G—PHYSICS
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- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/24—Coupling light guides
- G02B6/26—Optical coupling means
- G02B6/262—Optical details of coupling light into, or out of, or between fibre ends, e.g. special fibre end shapes or associated optical elements
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/24—Coupling light guides
- G02B6/26—Optical coupling means
- G02B6/32—Optical coupling means having lens focusing means positioned between opposed fibre ends
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- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
- G21K1/00—Arrangements for handling particles or ionising radiation, e.g. focusing or moderating
- G21K1/006—Manipulation of neutral particles by using radiation pressure, e.g. optical levitation
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Abstract
The invention provides fiber optical tweezers based on a special optical fiber and a system. The method is characterized in that: the large-core-diameter coaxial double-waveguide fiber laser comprises a coaxial double-waveguide fiber 1, a large-core-diameter coaxial double-waveguide fiber 2, a frustum 3, a Fresnel diffraction lens 4, a coaxial double-wave optical fiber connector 5 and lasers (6 and 7). In the system, lasers (6, 7) are respectively connected with a middle core and an annular core of a coaxial double-waveguide fiber 1 through a coaxial double-wave optical fiber connector 5. The other end of the coaxial double-waveguide fiber 1 is welded with a section of large-core-diameter coaxial double-waveguide fiber 2, the other end of the large-core-diameter coaxial double-waveguide fiber 2 is processed into a frustum 3, and light is converged into a potential well capable of stably capturing particles through the frustum 3; the middle core of the large-core-diameter coaxial double-waveguide fiber 2 is processed with a Fresnel diffraction lens 4, and light is converged into another potential well capable of stably capturing particles through the Fresnel diffraction lens 4. The invention can be used for capturing and ejecting particles and can be widely used in the fields of photodynamic control and the like.
Description
(I) technical field
The invention relates to a special optical fiber-based optical tweezers and a system, which can be used for capturing and ejecting cells or particles and belong to the technical field of photodynamic manipulation.
(II) background of the invention
In 1986, the concept of "Optical tweezers" was first proposed by Askin and his colleagues [ Optical Letters, 18 (5): 288-. Currently, the optical tweezers technology becomes a general tool for scientific research in the exploration of the micro world.
Optical tweezers, i.e. using light to grab an object. In contrast, optical tweezers are designed to provide a "grab" effect by optically confining particles. The optical tweezers can enable the spatial resolution to reach the nanometer magnitude, enable the force resolution to reach the piconiumber magnitude and enable the time resolution to reach the millisecond magnitude. This allows one to study life forms from single cell levels to single molecule levels. Developments over the past decades have transformed these tools from precision instruments to highly versatile molecular biophysical instruments. Currently, optical tweezers have become the most widely used high-precision positioning technology and technology for measuring the strength of a cow in bioscience.
Although conventional optical tweezers have achieved significant results, their use is still limited in a considerable number of environments. For example, it is difficult to focus a light beam in a thick sample or turbid medium using conventional optical tweezers systems, which typically have a small working distance. In addition, cost and portability issues are obstacles to their widespread use.
In order to solve the above problems, 2016, suchenguang et al disclose an automatic microsphere capturing method (application number: 201610832084.4) in an optical tweezers system, which can automatically identify and capture particles of 800nm to 10 μm, effectively improve the efficiency of experiments, reduce the workload of experimenters, and improve the stability of experimental data. In the same year, Huangwei et al disclosed a four-core helical fiber-based fiber optical tweezers and a method for making the same (application number: 201610412841.2). And after the four-core optical fiber is twisted, a spiral optical fiber is formed, so that the optical tweezers of the optical fiber are formed. In 2017, Zhangyonghui et al disclose a step multimode fiber optical tweezers (application number: 201721083580.0) based on an oblique annular light field, wherein an annular light field completely composed of oblique light is constructed in a fiber core of a multimode fiber, and a light intensity gradient field is formed after a fiber head is ground, so that cells or micro-droplets in a flying level are captured.
In addition, the applicant discloses a throughput type optical fiber optical tweezers based on a coaxial dual-waveguide structure in 2010 and a preparation method (application number: 201010215424.1). The particles are controlled by the coaxial double waveguide fibers, and the throughput, emission and even suck-back of the particles can be stably captured by adjusting and changing the light power of the light source. Meanwhile, the particle capture is more flexible and accurate, the adjustability is realized, and the practicability of the optical fiber tweezers technology is greatly improved.
Although the above patents can perform operations such as capturing and handling on particles, most of them can only operate one particle at a time, and their functions are relatively single.
The invention discloses a special optical fiber-based optical tweezers. Can be used for capturing and ejecting cells or particles, and can be widely used in the fields of cell manipulation and the like. And a small section of large-core-diameter coaxial double-waveguide fiber is welded at one end of the small-core-diameter coaxial double-waveguide fiber. The large-core-diameter coaxial double-wave light guide fiber end is ground into a frustum, so that the annular cores can converge to form a three-dimensional stable capture potential well. The Fresnel lens is carved on the middle core of the large-core-diameter coaxial double-waveguide fiber by the femtosecond laser processing technology, so that the middle core can also form a stable focused light field. Compared with the prior art, the invention has the advantages that: on one hand, the annular core forms a focus through the frustum, the middle core forms a focus point through the diffraction lens, and two particles with a certain distance can be captured simultaneously; the intermediate core and the annular core may be optically transparent, respectively, so that only one particle is captured at a time. On the other hand, by designing the focal length of the intermediate core binary diffraction lens, the focal length of the lens is close to that of the annular core, so that the annular core can be used for capturing particles, and the intermediate core is used for ejecting the particles; the intermediate core is a light beam converged by the diffraction lens, the light beam is compressed, and the ejection acceleration area is longer than that of a directly emergent Gaussian light beam.
Disclosure of the invention
The invention aims to provide multifunctional composite optical fiber tweezers and a system, which have simple structure, capture single or multiple cells and have ejection function.
The purpose of the invention is realized as follows:
the large-core-diameter coaxial double-waveguide fiber laser comprises a coaxial double-waveguide fiber 1, a large-core-diameter coaxial double-waveguide fiber 2, a frustum 3, a fiber end Fresnel diffraction lens 4, a coaxial double-wave optical fiber connector 5 and lasers (6 and 7). The laser 6 is connected to the intermediate core 101 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5, and the laser 7 is connected to the annular core 102 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5. The other end of the coaxial double-waveguide fiber 1 is welded with a section of large-core-diameter coaxial double-waveguide fiber 2, the other end of the large-core-diameter coaxial double-waveguide fiber 2 is ground into a frustum, and light transmitted by the annular core 202 is converged to form a potential well capable of stably capturing particles; the Fresnel diffraction lens 4 is engraved on the end surface of the middle core of the large-core-diameter coaxial double-waveguide fiber 2 by the femtosecond laser processing system, so that light transmitted by the middle core 201 is converged into another potential well capable of stably capturing particles through the Fresnel diffraction lens 4.
The coaxial double-waveguide fiber 1 has an outer diameter D1Preferably, D1And 125 μm. It is composed of an intermediate waveguide 101 and an annular core waveguide 102, the diameter of the intermediate waveguide 101 is A1Preferably A 110 μm, and an inner diameter B of the annular core waveguide 1021Outer diameter of C1Preferably, B184 μm and an outer diameter of C1And 94 μm. The intermediate core waveguide 101 and the annular core waveguide 102 are coaxial.
The coaxial double waveguide fiber 2 has an outer diameter D2Preferably, D2And 125 μm. It is composed of an intermediate core waveguide 201 and a ring core waveguide 202, the diameter of the intermediate core waveguide 201 is A2Preferably A270 μm, and an inner diameter B of the annular core waveguide 22Outer diameter of C2Preferably, B2Is 84 μm, C2And 94 μm. The intermediate core waveguide 201 and the annular core waveguide 202 are coaxial. The annular core of the coaxial double-waveguide fiber 2 is equal to the annular core of the coaxial double-waveguide fiber 1 in size.
The length of the coaxial double waveguide fiber 2 is L, preferably, L is 300 μm.
Zhang et al (Zhang Y, Liu Z, Yang J, et al, an annular core single fibers tweezers [ J ]. Sensor Letters,2012,10(7):1374-1377.) analyzed the magnitude of the optical trapping force on the ring core fiber at different polishing angles by using the finite element method. Finally, it is concluded that when the included angle of the conical outgoing light beam from the annular core fiber optical tweezers is a right angle, the amplitudes of the axial and transverse light trapping forces are the largest, that is, when the included angle of the annular light beam approaches the right angle, the optical trapping capability of the fiber optical tweezers probe is the strongest on the premise that the light source power, the refractive index of the trapped particles and the size are the same.
The frustum 3 is formed by finely grinding the optical fiber end of the large-core diameter coaxial double-waveguide optical fiber 2, and the cone angle is beta, and preferably the beta is 17 degrees.
The end face diameter of the large core coaxial double waveguide fiber 2 after polishing is D, preferably, D is 80 μm.
The Fresnel diffraction lens 4 at the optical fiber end is directly processed on the middle core 201 of the large-core-diameter coaxial double-waveguide optical fiber 2 by using a femtosecond laser micromachining technology. The radius R of the wave band is determined by Fresnel equation, the optical path difference of the adjacent wave bands is lambda, and the radius of the nth wave band can be obtained by geometric optics knowledge as follows:
wherein f is0Is the principal focal length, λ, corresponding to the first diffraction order0Is the design wavelength.
When f is0＞＞λ0Then the radius of the nth band is approximately:
design wavelength lambda used in the system0980nm, focal length f0And was 45 μm.
Radius r of the Fresnel diffraction lens 4nWherein n is 12, r1To r12The values (unit: μm) of (d) are respectively: 6.6, 9.4, 11.5, 13.28, 14.85, 16.27, 17.57, 18.78, 19.92, 21, 22.02, 23.
Manufacturing the Fresnel diffraction lens 4: and etching a ring groove with the depth of d on the even half-wave band by using a femtosecond laser micro-processing system to generate pi phase difference with the odd half-wave band, wherein d is preferably 3.66 mu m.
Compared with the prior art, the invention has the outstanding advantages that:
(1) single particles and a plurality of particles are collected and captured, the ejection function is realized on one optical fiber, the integration level is high, the structure is simple, and the operation is flexible and convenient.
(2) The focal length of the binary diffraction lens with the middle core is designed to enable the focal length of the lens to be close to that of the annular core, the annular core is used for capturing particles, the middle core is used for ejecting the particles, the middle core is a light beam converged by the diffraction lens, and compared with a Gaussian light beam ejection particle which is directly emitted, an ejection acceleration area is longer.
(IV) description of the drawings
Fig. 1 is a system structure diagram of a fiber optical tweezers and system based on special optical fiber. The optical fiber laser comprises a coaxial double-waveguide optical fiber 1, a large-core-diameter coaxial double-waveguide optical fiber 2, a frustum 3, an optical fiber end Fresnel diffraction lens 4, a coaxial double-wave optical fiber connector 5 and lasers (6 and 7). 101 is the middle core of the coaxial double- waveguide fiber 1, and 102 is the annular core of the coaxial double-waveguide fiber 1; 201 is the intermediate core of the large-core coaxial double- waveguide fiber 2, and 202 is the annular core of the large-core coaxial double-waveguide fiber 2. 401 is where the femtosecond laser is processed on the fiber end face.
Fig. 2 is an end view of a coaxial double waveguide fiber 1 of a fiber optical tweezers and system based on special fiber.
Fig. 3 is an end view of a coaxial double waveguide fiber 2 of a fiber optical tweezers and system based on special fiber.
Fig. 4 is a schematic diagram of particles captured by the annular fiber core 202 of the fiber optical tweezers and system based on special optical fibers, wherein 5 is a convergent beam of an outgoing optical field of the annular core, 6 is the captured particles, and 8 is a schematic diagram of a transmission direction of light in the annular core.
Fig. 5 is a graph of the lateral optical trapping force experienced by particles trapped by the fiber optical tweezers and the ring core 202 of the system based on a special optical fiber.
Fig. 6 is a graph showing the axial optical trapping force applied to the particles captured by the fiber optical tweezers and the ring core 202 of the system based on the special optical fiber.
Fig. 7 is a schematic diagram of the special fiber-based optical tweezers and the system, in which light transmitted through the intermediate core 201 captures particles through the fresnel diffraction lens 4, where 7 is a converging beam, 6 is the captured particles, and 8 is a schematic diagram of the direction of light transmitted through the intermediate core.
Fig. 8 is a graph showing the lateral light trapping force exerted on the particles captured by the fresnel diffraction lens 4 by the optical fiber optical tweezers based on the special optical fiber and the system intermediate core 201.
Fig. 9 is a graph showing an axial optical trapping force applied to particles captured by the fresnel diffraction lens 4 by light transmitted through the fiber optical tweezers based on a special optical fiber and the system intermediate core 201.
FIG. 10 is a schematic diagram of a special fiber-based optical tweezers and system for capturing two particles.
Fig. 11 is a schematic diagram of a special fiber-based fiber optical tweezers and a system for realizing the ejection function.
Fig. 12 shows a light field propagation condition after light transmitted by the special fiber-based optical fiber tweezers and the system intermediate core 201 is converged by the fresnel diffraction lens 4.
(V) detailed description of the preferred embodiments
The invention is further illustrated below with reference to specific examples.
Example 1: a process for preparing optical tweezers based on special optical fibers.
Step 1: taking sections of the coaxial double-waveguide fiber 1 and the large-core-diameter coaxial double-waveguide fiber 2 respectively, removing the coating layer, cutting the end face of the fiber by a cutter, and wiping the fiber by alcohol.
Step 2: and putting the two sections of optical fibers into an optical fiber fusion splicer for fusion splicing.
And step 3: and cutting the large-core-diameter coaxial double-waveguide fiber 2 by using a fixed-length cutting system to enable the length of the large-core-diameter coaxial double-waveguide fiber 2 to be 300 mu m.
And 4, step 4: wiping the welded optical fiber with alcohol to remove dust, and then placing the optical fiber on a displacement table of a femtosecond micro-processing system;
and 5: setting the frequency to be 60kHz, setting the power to be 0.5mW, selecting an objective lens with the numerical aperture of 0.42 multiplied by 50, and focusing the femtosecond laser to the end surface of the optical fiber through a microscope objective lens;
step 6: drawing a graph on the upper computer software written by the user, generating an executable code, and executing the code. After the execution is finished, the optical fiber end has two parts, one part is an unmodified part, and the other part is a modified part;
and 7: and (3) placing the sample processed by the femtosecond laser scanning into a hydrofluoric acid solution with the concentration of 5%, and carrying out auxiliary corrosion on the sample for about 25min by using an ultrasonic cleaning machine.
And 8: and (3) putting the optical fiber sample obtained in the step (7) into an optical fiber grinding machine to grind a frustum 3, wherein the angle is 17 degrees.
Example 2: and (4) capturing single particles.
Fig. 4 is a schematic diagram of a particle captured after light transmitted in the toroidal core is converged by a frustum. And (3) turning on the laser 7, coupling the light in the laser 7 into the annular core 102 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5, transmitting the light into the annular core 202 of the large-core-diameter coaxial double-waveguide fiber 2, and finally converging the light through the frustum 3 to form a good three-dimensional capture potential well.
For the structure of the trapped particle of fig. 4, a force simulation was performed with a finite element method pellet, where the background index is 1.33, the pellet index is 1.41, the refractive indices of the toroidal cores 102, 202 are 1.46, and the frustum angle is 17 °. The results are shown in fig. 5 and 6. FIG. 5 is a graph of the lateral optical trapping force experienced by the pellet, which can be seen to range from-20 μm to 20 μm lateral trapping. FIG. 6 is a graph of axial optical trapping force experienced by a pellet with a lateral trapping range of 20 μm to 60 μm and a focal point at 50 μm.
Fig. 7 is a schematic diagram of the trapped particles after the intermediate core has been converged by the fresnel diffractive lens 4. And (3) turning on the laser 6, coupling the light in the laser 6 into the middle core 101 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5, transmitting the light into the middle core 201 of the large-core-diameter coaxial double-waveguide fiber 2, and finally converging the light through the Fresnel diffraction lens 4 to form a focus capable of capturing cells.
For the structure of the trapped particle of fig. 7, the force of the pellet was simulated by the finite element method, wherein the refractive index of the background was 1.33, the refractive index of the pellet was 1.41, the refractive index of the intermediate core was 1.45, and the focal length of the lens was 20 μm, and the results are shown in fig. 8 and 9. FIG. 8 is a graph of the lateral optical trapping force experienced by a pellet, which can be seen to range from-30 μm to 30 μm lateral trapping. FIG. 9 is a graph of the axial optical trapping force experienced by a pellet with a lateral trapping range of 0 μm to 40 μm.
Example 3: and (4) capturing double particles.
Fig. 10 is a schematic diagram of two particles captured simultaneously, and the focal length of the fresnel diffraction lens 4 is designed to be 20 μm. The laser 6 and the laser 7 are switched on simultaneously. The light in the laser 6 is coupled into the middle core 101 of the coaxial double waveguide fiber 1 through the coaxial double-wave optical fiber connector 5; and then transmitted to the middle core 201 of the large-core diameter coaxial double-waveguide fiber 2, and finally converged by the Fresnel diffraction lens 4 to form a three-dimensional potential well capable of stably capturing particles. The light in the laser 7 is coupled to the annular core 102 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5, then is transmitted to the annular core 202 of the large-core-diameter coaxial double-waveguide fiber 2, and then is converged through the frustum 3 to form a three-dimensional potential well capable of stably capturing particles.
Example 4: particles eject embodiments.
Fig. 11 is a schematic view of particle ejection. The focal length of the Fresnel diffraction lens 4 is designed to be 50 μm, so that the distance between the Fresnel diffraction lens and a potential well formed by the annular core light beam converged by the frustum 3 is equal. And (3) turning on the laser 7, coupling the light in the laser 7 into the annular core 102 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5, transmitting the light into the annular core 202 of the large-core-diameter coaxial double-waveguide fiber 2, and converging the light through the frustum 3 to form a three-dimensional potential well capable of stably capturing particles. After the particles are stably captured, the laser 6 is turned on, and the light in the laser 6 is coupled into the middle core 101 of the coaxial double-waveguide fiber 1 through the coaxial double-wave optical fiber connector 5; and then the optical fiber is transmitted into the middle core 201 of the large-core-diameter coaxial double-waveguide optical fiber 2, and finally the optical fiber is converged by the Fresnel diffraction lens 4 to form a long focus which acts on the small ball, so that the small ball is accelerated to be ejected out under stress. Fig. 12 shows the distribution of the light field after being converged by the fresnel diffraction lens 4, and it can be seen that after passing through the fresnel diffraction lens 4, an elongated focal point is formed, which has a length of about 10 μm and a width of about 2 μm.
Claims (6)
1. The utility model provides a fiber optic tweezers and system based on special optical fiber, it comprises coaxial double waveguide fiber (1), the coaxial double waveguide fiber (2) of big core footpath, frustum (3), fiber end fresnel lens (4), coaxial two ripples fiber connector (5), laser instrument (6, 7), in the system, laser instrument (6) link to each other with middle core (101) of coaxial double waveguide fiber (1) through coaxial two ripples fiber connector (5), and laser instrument (7) link to each other with annular core (102) of coaxial double waveguide fiber (1) through coaxial two ripples fiber connector (5). The other end of the coaxial double-waveguide fiber (1) is welded with a section of large-core-diameter coaxial double-waveguide fiber (2), the other end of the large-core-diameter coaxial double-waveguide fiber (2) is ground into a frustum (3), so that light transmitted by the annular core (202) is converged to form a potential well capable of stably capturing particles; the end face of the middle core of the large-core-diameter coaxial double-waveguide fiber (2) is provided with the Fresnel lens (4), so that light transmitted by the middle core (201) can be converged into another potential well capable of stably capturing particles through the Fresnel lens (4).
2. The fiber optical tweezers and the coaxial double waveguide fiber (1) in the system based on the special fiber according to claim 1, wherein: the size of the annular core (102) is the same as that of the annular core (202) of the large-core-diameter coaxial double-waveguide fiber (2).
3. The special optical fiber-based optical tweezers and the large-core-diameter coaxial double-waveguide optical fiber (2) in the system according to claim 1, wherein: the large-core diameter coaxial double-waveguide fiber (2) can also be a coreless fiber.
4. The special optical fiber-based optical tweezers and system frustum (3) according to claim 1, wherein: the frustum (3) is directly processed at one end of the coaxial double-waveguide fiber (2).
5. The special optical fiber-based optical tweezers and system frustum (3) according to claim 1, wherein: the angle interval of the cone angle is 0 DEG to 60 deg.
6. The special fiber-based fiber optical tweezers and the fiber end Fresnel diffraction lens (4) in the system according to claim 1. The method is characterized in that: the etching depth of the even half-wave band of the femtosecond laser processing is 3.66 mu m.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113917609A (en) * | 2021-10-12 | 2022-01-11 | 桂林电子科技大学 | Large-core-diameter coaxial double-waveguide optical fiber fan-in fan-out device for reflection spectrum measurement |
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Application publication date: 20201211 Assignee: Guilin Photon Power Technology Co.,Ltd. Assignor: GUILIN University OF ELECTRONIC TECHNOLOGY Contract record no.: X2023980045805 Denomination of invention: A Fiber Optic Tweezer and System Based on Special Fiber Granted publication date: 20220513 License type: Common License Record date: 20231105 |