CN107300538A - A kind of unicellular accurate test experience device based on optical fiber - Google Patents
A kind of unicellular accurate test experience device based on optical fiber Download PDFInfo
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- 239000013307 optical fiber Substances 0.000 title claims abstract description 86
- 238000012360 testing method Methods 0.000 title claims abstract description 20
- 239000000835 fiber Substances 0.000 claims abstract description 157
- 239000000523 sample Substances 0.000 claims abstract description 37
- 238000012576 optical tweezer Methods 0.000 claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 17
- 230000003287 optical effect Effects 0.000 claims abstract description 5
- 238000001228 spectrum Methods 0.000 claims abstract description 5
- 238000000227 grinding Methods 0.000 claims description 21
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N2021/258—Surface plasmon spectroscopy, e.g. micro- or nanoparticles in suspension
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Abstract
The invention discloses a kind of unicellular accurate test experience device based on optical fiber, the device is made up of super continuous spectrums single-mode output light source with programmable optical spectrometer, detection probe is made up of micro-structural multi-core fiber, simultaneously optical fiber optical tweezers are made using micro-structural multi-core fiber, it is controlled to unicellular, under the microscope, unicellular on objective table is accurately laid on by optical tweezer on micro-structural multicore SPR probe circular cone structure detection platforms.Micro-structural multi-core fiber diameter only has 125 μm, and spr sensor probe and optical tweezer is made into circular cone structure in abrading tip, suitable with unicellular tens micron dimension, can be well be used for precisely medical treatment in it is single celled it is accurate capture control with refractive index detect.
Description
Technical field
The invention belongs to accurate medical research experiment equipment field, and in particular to a kind of unicellular accurate inspection based on optical fiber
Detection experiment apparatus.
Background technology
With the completion of the Human Genome Project, individual human genome, Oncogenome, environmental genomics, gene sequencing
The development of technology, and biological and medical science is towards the progressively conversion of data-intensive science, " precisely medical treatment " is used as biological and doctor
One novel concept in field is arisen at the historic moment, and positive guidance is provided for clinical disease more accurate and effective diagnosis, treatment
Effect.Precisely the implementation of medical treatment can promote medical science and technology and the further mixing together of big data and information science, so that
Medical model changes from extensive style to accurate.Therefore, domestic and international national governments and enterprise have had begun to " precisely medical treatment " back of the body
Strategy under scape is disposed with scientific research.The Chinese strategic expert group of precisely medical treatment had been set up already in Chinese Government, actively formulated " precisely
Medical treatment " strategical planning, and plan to include " 13 " major scientific and technological project.By taking malignant tumour as an example, current diagnosis it is main according to
Rely imageological examination, pathological examination and tumor markers etc., with the arrival in accurate medical epoch, unicellular control and detection
Technology is arisen at the historic moment, it has also become the study hotspot of tumour educational circles, detection of the mankind to malignant tumour is reached individual cell level.
Refractive index is as medical test parameter in occupation of increasingly consequence, and people can be from the refractive index of environment
Some characteristics of material are analyzed, and the refractive index of biological cell is even more then a weight of reflection light and cell interaction property
Physical quantity is wanted, is a basic parameter in many Photobiology ecsomatics experiments.Such as carried out with spectroscopy of polarized light scattering cancer,
Particularly the experimental study of early-stage cancer checkout and diagnosis when, it is necessary to which the relative index of refraction of given cell, carries out spectra inversion, ability
The shape information of cell is obtained from spectroscopic data accurate quantification.Because SPR resonance curves become to the refractive index of testing medium
Change is very sensitive, and small variations in refractive index can make resonance condition occur obvious change, can by the change for measuring resonance curve
To determine the variations in refractive index of testing medium, based on the Cleaning Principle, the technology can be applied in medical detection field.
Lung cancer is a kind of high malignant tumour of fatal rate, shows that lung cancer is sent out in Report of Chinese Cancer Control Strategy
Sick rate increases year by year, seriously threatens the health of people.Current CT image checks are the Main Means for improving the survival rate of patient.
Show there is higher false positive rate and mistake by the result of Qualitative Imaging Diagnosis according to existing clinical imageology diagnostic result
Examine rate.Different material is to the essence that the difference of scattering and the absorption of light is CT image technologies, and this species diversity is that light and material are mutual
Optical heterogeneity caused by effect.Macroscopic view and the biological tissue optical heterogeneity of micro-scale, correspondence main theory are different.
On molecular level and cell dimensions, electric susceptibility and refractive index are then the standard for weighing this inhomogeneities respectively.As can be seen here, it is right
The definite understanding of lung carcinoma cell refractive index is significant to the accuracy rate for improving CT image etiologic diagnosis.But now for lung
The data report of the unicellular refractive index of cancer is very few, thus it is particularly significant to carry out experimental study to its single celled refractive index.
Because most of biological tissue has stronger scattering and absorption, traditional survey based on the law of refraction to visible ray
The method for measuring transparent substance refractive index is no longer applicable.At present, the method to Refractive Index Measurement of Bio-tissue mainly has bare fibre spy
Pin method of testing, optical coherence tomography (OCT) method, total reflection method and refractometry is attributed to reflection using fresnel formula
Measuring method of rate etc.；Bare fibre probe method, is, as the covering of fiber core, to pass through measurement " optical fiber " using sample
Numerical aperture, so that it is determined that the refractive index of sample.It has the disadvantage, if fibre core and sample loose contact or sample size compared with
Small, this method will fail；OCT methods are to be combined together low coherence interferometer with confocal scanning imaging technology, many by filtering out
Secondary scattered light, so that the refractive index of sample is relatively accurately measured, but this method light path is complicated, is difficult regulation.
The content of the invention
In consideration of it, the present invention provides a kind of unicellular accurate test experience device based on optical fiber, to list on objective table
The accurate medical experiment research that cell is captured and detected.
The purpose of the present invention realizes that a kind of unicellular accurate detection based on optical fiber is real by such technical scheme
Experiment device, including super continuum source 1, coupled lens group 4, micro-structural multi-core fiber I5-1, micro-structural multi-core fiber II5-3,
Spectrometer 6,980nm light sources 7, objective table 8 and electron microscope 9, the objective table are used to carry cell to be detected；Micro- knot
Structure multi-core fiber I tail ends are provided with optical fiber pickup probe 5-2, and the micro-structural multi-core fiber II tail ends are provided with optical fiber light
Tweezer 5-4；The light injection single-mode fiber I2-1 that super continuum source 1 is sent, then the injection micro-structural multi-core fiber of coupled lens group 4
I5-1, flashlight is redirected back into spectrometer 6 by single-mode fiber II2-2 and entered in optical fiber pickup probe 5-2 end face reflection
Row detection data processing；The light that 980nm light sources 7 are sent injects micro-structural multi-core fiber II5-3 by single-mode fiber III2-3, in light
Fine optical tweezer 5-4 end face outgoing is simultaneously converged, and forms unicellular ligh trap capture field；Under the observation of electron microscope 9, optical fiber optical tweezers are grabbed
Pick and place be placed in it is to be detected unicellular on objective table, give and optical fiber pickup probe detect.
Further, the optical fiber optical tweezers are prepared by the following：One section of length of interception is that 2m, outer cladding diameter are 125 μm,
The extraordinary annular core fibre of micro-structural, removes coat 2cm using Miller pincers at annular core fibre two ends, alcohol is dipped with non-woven fabrics
Wiped clean, and it is using optical fiber cutter that its ends cutting is smooth, using optical fiber end grinding technique by annular core fibre one end
Grind to form the frustum of a cone at 37.5 ° of inclination angles, grinding depth is 34.5 μm, makes optical fibre ring, by annular core fibre other end with
Single-mode fiber dislocation welding, makes single-mode fiber fibre core just to toroidal cores fiber core certain point, single-mode fiber and capture light
Source tail optical fiber is welded to connect firmly by electric discharge.
Further, the optical fiber pickup probe is prepared by the following：It is 125 μm, micro-structural by outer cladding diameter
Extraordinary twin-core fiber end face is cut flat with, and is clamped in optical fiber lapping system, and white light is passed through simultaneously in two cores, is easy to by grinding system
The CCD of system positions two fibre cores of optical fiber；Twin-core fiber is axially rotated, makes two fibre cores place straight line perpendicular to abrasive disk, under
Press polish is fine, makes itself and abrasive disk angulation, is ground；When being ground to projected depth, upper lift optical fiber, vertically by fiber spinning
180 degree, pushes optical fiber and is extremely ground with abrasive disk into 37.5 ° of angles again；It is fine grinding side during second of grinding
Single-frequency laser is passed through in core, light intensity is monitored in opposite side fibre core, when light intensity reaches maximum, stops grinding, light is in twin-core
Pass through triple reflection at the cone angle structure that fiber end face is ground to form, having returned to reflected light from incident fibre core collects fibre core；It will grind
The twin-core fiber cone angle sound end finished is placed upwardly on below ion sputtering instrument gold target, is coated with 500nm thick gold membranes and is formed instead
Penetrate film, it is ensured that light can reflex to outgoing fibre core by incident fibre core in aqueous；Cone angle is carefully removed with optical fiber grinding system
Probe top surface golden film, and 50nm thick gold membranes are coated with cone angle probe top surface, it is used as surface plasma body resonant vibration film.
Further, the device also includes being used to adjust the three-dimensional adjusting module I3-1 of single-mode fiber I positions, for adjusting list
The three-dimensional adjusting module II3-2 of the mode fiber II positions and three-dimensional adjusting module III3-3 for adjusting single-mode fiber III positions.
Further, the single-mode fiber II is 532nm, 633nm, 980nm or 1310/1550nm single-mode fiber.
Further, the coupled lens group is fiber coupling object lens, and multiple is 4 times or 10 times.
Further, the coupled lens group is waveguide fan-out-type coupled apparatus, for by single-mode fiber I light beam and single mode
Optical fiber II beam separation is parallel to be compressed to the axial symmetry fibre core spacing of micro-structural multi-core fiber I two.
Further, the fibre core number of the micro-structural multi-core fiber I5-1 is even number, and fibre core is axisymmetricly distributed.
Further, the fibre core number of the micro-structural multi-core fiber II5-3 is odd number, and fibre core is axisymmetricly distributed, single-mode optics
Fine III is by drawing cone method to be micro-structural multi-core fiber II coupling note light.
By adopting the above-described technical solution, the present invention has the advantage that：
The present invention proposes a kind of unicellular accurate test experience device based on optical fiber, in accurate medical field, makes thin
Born of the same parents control to reach individual cell level with the detection of highly sensitive refractive index.The present invention can real-time, undamaged measurement and analysis list
The variations in refractive index of cell, so as to carry out the existence and pathological condition research of cell；Its extraordinary micro-structural multi-core fiber used
Optical tweezer is less than 100 μm with unicellular SPR refractive indexes detection probe circular cone mesa diameter, with conventional unicellular size in same amount
Level, has broad application prospects in accurate medical research experiment field.
Brief description of the drawings
In order that the object, technical solutions and advantages of the present invention are clearer, below in conjunction with accompanying drawing the present invention is made into
The detailed description of one step, wherein：
Fig. 1 is overall structure composition schematic diagram of the invention；
Fig. 2 is unicellular SPR refractive indexes detection light path schematic diagram of the invention；
Fig. 3 is unicellular ligh trap capture field light path schematic diagram of the invention.
Embodiment
Below with reference to accompanying drawing, the preferred embodiments of the present invention are described in detail；It should be appreciated that preferred embodiment
Only for the explanation present invention, the protection domain being not intended to be limiting of the invention.
As shown in figure 1, a kind of unicellular accurate test experience device based on optical fiber, including super continuum source 1, coupling
Lens group 4, micro-structural multi-core fiber I5-1, micro-structural multi-core fiber II5-3, spectrometer 6,980nm light sources 7, objective table 8 and electricity
Sub- microscope 9, the objective table is used to carry cell to be detected；The micro-structural multi-core fiber I tail ends are provided with optical fiber biography
Feel probe 5-2, the micro-structural multi-core fiber II tail ends are provided with optical fiber optical tweezers 5-4；The light injection that super continuum source 1 is sent
Single-mode fiber I2-1, then the injection micro-structural multi-core fiber I5-1 of coupled lens group 4, flashlight is in optical fiber pickup probe 5-2
End face reflection, by single-mode fiber II2-2 be redirected back into spectrometer 6 carry out detection data processing；What 980nm light sources 7 were sent
Light by single-mode fiber III2-3 inject micro-structural multi-core fiber II5-3, optical fiber optical tweezers 5-4 end face outgoing and converge, formed
Unicellular ligh trap captures field；Under the observation of electron microscope 9, optical fiber optical tweezers crawl is positioned over to be detected slender on objective table
Born of the same parents, send and are detected with optical fiber pickup probe.
In the present embodiment, described super continuum source 1 is NKT Photonics Superk Compact models,
Wave-length coverage 450-2400nm, general power is more than 100mW, and 0-100% is adjustable.
In the present embodiment, described single-mode fiber II is 532nm, 633nm, 980nm or 1310/1550nm single-mode optics
It is fine.
In the present embodiment, test experience device also includes the three-dimensional adjusting module I3- for being used to adjust single-mode fiber I positions
1st, for adjusting the three-dimensional adjusting module II3-2 of single-mode fiber II positions and being adjusted for adjusting the three-dimensional of single-mode fiber III positions
Mould preparation block III3-3.The MBT602 that three-dimensional adjusting module I3-1 and three-dimensional adjusting module II3-2 are Thorlabs, three-dimensional adjustment mould
Block I3-3 is Thorlabs MBT610D models.
In the present embodiment, described coupled lens group 4 can be fiber coupling object lens, and multiple is 4 times or 10 times；Also may be used
Think that waveguide is fanned out to shape coupled apparatus, for parallel to be compressed to micro-structural multi-core fiber two symmetrical by two single-mode fiber beam separations
Apart from size between core.
In the present embodiment, micro-structural multi-core fiber I5-1 cladding diameters are 125 μm, and fibre core number is even number, such as twin-core, four
Core or six core fibres, fibre core are axisymmetricly distributed, and optical fiber pickup probe 5-2 is to enter in micro-structural multi-core fiber I5-1 tail ends
Row bare fibre grinds to form circular cone structure and is coated with 50nm golden films and is made；Micro-structural multi-core fiber II5-3 cladding diameters are 125 μ
M, fibre core number is odd number, and such as three cores, five cores or annular core fibre, fibre core are axisymmetricly distributed, and single-mode fiber III2-3 is by drawing
Cone method is its coupling note light；Optical fiber optical tweezers 5-4 is to utilize bare fibre grinding, oxyhydrogen flame in micro-structural multi-core fiber II5-3 tail ends
Draw cone or hydrofluoric acid to corrode into truncated cone shape, make multi-beam converge to form unicellular ligh trap capture field.
The making to unicellular accurate test experience device building block is described further below.
1st, unicellular capture optical fiber optical tweezers are made：One section of length of interception is that 2m, outer cladding diameter are 125 μm, and micro-structural is extraordinary
Annular core fibre, removes coat 2cm using Miller pincers at its two ends, alcohol wipe is dipped with non-woven fabrics totally, and use light
Fine cutter are smooth by its ends cutting, and optical fiber one end is ground to form to the frustum of a cone at 37.5 ° of inclination angles using optical fiber end grinding technique,
It is 34.5 μm to grind depth (i.e. frustum of a cone height), the toroidal cores optical fiber optical tweezers structure completed and its outgoing light field such as Fig. 3
It is shown.Optical fibre ring other end and single-mode fiber are misplaced and welded, makes single-mode fiber fibre core just to toroidal cores fiber core
A bit, single-mode fiber is welded to connect firmly with capture light source tail optical fiber by electric discharge.
2nd, optical fiber pickup probe is made：It is 125 μm by outer cladding diameter, micro-structural special type twin-core fiber end face is cut flat with,
It is clamped in optical fiber lapping system, white light is passed through simultaneously in two cores, is easy to position two of optical fiber by the CCD of grinding system
Fibre core.Twin-core fiber is axially rotated, makes straight line where two fibre cores perpendicular to abrasive disk, pushes optical fiber, makes it with abrasive disk into α
=37.5 ° of angles, are ground.When being ground to projected depth, upper lift optical fiber, vertically by fiber spinning 180 degree, is pushed again
Optical fiber is extremely ground with abrasive disk into 37.5 ° of angles.During second of grinding, single-frequency is passed through in side fibre core has been ground and is swashed
Light, light intensity is monitored in opposite side fibre core, when light intensity reaches maximum, stop grinding, light twin-core fiber end surface grinding into
Cone angle structure at pass through triple reflection, from incident fibre core returned to reflected light collect fibre core.The twin-core fiber finished will be ground
Cone angle sound end is placed upwardly on below ion sputtering instrument gold target, is coated with 500nm thick gold membranes formation reflectance coating, it is ensured that water-soluble
Light can reflex to outgoing fibre core by incident fibre core in liquid.Cone angle probe top surface golden film is carefully removed with optical fiber grinding system, and
50nm thick gold membranes are coated with cone angle probe top surface, surface plasma body resonant vibration film is used as.
3rd, experimental provision integral manufacturing：Two single-mode fibers for being about 1m are taken, two ends remove coat using Miller pincers
2cm, alcohol wipe is dipped with non-woven fabrics clean and using optical fiber cutter that its ends cutting is smooth；Optical fiber after handling well
After being clamped with naked fibre adapter, a connection light source, a connection spectrometer, by two single-mode fiber other ends and twin-core light
Fine planar end surface one end is placed in precision three-dimensional adjustment frame, by coupled lens group be fixed on single-mode fiber and twin-core fiber it
Between, adjusted by calibrating, realize paths effect as shown in Figure 2, spectrometer is preferably received after detection
Flashlight.
4th, device is operated：The optical fiber pickup probe that twin-core fiber is made contains cell as on slide, taking on a small quantity
Nutrient solution be added dropwise on probe, submerge optical fiber pickup probe, then by toroidal cores optical fiber optical tweezers with precision three-dimensional adjust mould
The micro- behaviour of block is moved under environmental liquids liquid level, under electron microscope auxiliary, makes optical fiber optical tweezers confocal flat with optical fiber pickup probe
Face, and in the case where micro- behaviour manipulates, capture individual cells with optical fiber optical tweezers, reclined to optical fiber pickup probe end face sensing unit,
Light source and spectrometer are opened, after system is stable, sensing spectral line is obtained by spectrometer.
The present invention proposes a kind of unicellular accurate test experience device based on optical fiber, and detection device is by super continuous spectrums single mode
Output light source is constituted with programmable optical spectrometer, and detection probe is made up of micro-structural multi-core fiber, while using micro-structural multi-core optical
Fibre makes optical fiber optical tweezers, is controlled to unicellular, under the microscope, and unicellular on objective table is accurately laid by optical tweezer
In on micro-structural multicore SPR probe circular cone structure detection platforms.Micro-structural multi-core fiber diameter only has 125 μm, abrading tip
Spr sensor probe and optical tweezer is made into circular cone structure, suitable with unicellular tens micron dimension, can be well be used for is smart
Single celled accurate capture control is detected with refractive index in quasi- medical treatment.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, it is clear that those skilled in the art
Member can carry out various changes and modification to the present invention without departing from the spirit and scope of the present invention.So, if the present invention
These modifications and variations belong within the scope of the claims in the present invention and its equivalent technologies, then the present invention is also intended to include these
Including change and modification.
Claims (9)
1. a kind of unicellular accurate test experience device based on optical fiber, it is characterised in that：Including super continuum source (1), coupling
Close lens group (4), micro-structural multi-core fiber I (5-1), micro-structural multi-core fiber II (5-3), spectrometer (6), 980nm light sources
(7), objective table (8) and electron microscope (9), the objective table are used to carry cell to be detected；The micro-structural multi-core fiber I
Tail end is provided with optical fiber pickup probe (5-2), and the micro-structural multi-core fiber II tail ends are provided with optical fiber optical tweezers (5-4)；It is super
The light injection single-mode fiber I (2-1) that continuous spectrum light source (1) is sent, then coupled lens group (4) injection micro-structural multi-core fiber I
(5-1), flashlight is redirected back into spectrum in optical fiber pickup probe (5-2) end face reflection by single-mode fiber II (2-2)
Instrument (6) carries out detection data processing；The light that 980nm light sources (7) are sent injects micro-structural multi-core optical by single-mode fiber III (2-3)
Fine II (5-3), optical fiber optical tweezers (5-4) end face outgoing and converge, form unicellular ligh trap capture field；In electron microscope
(9) observe under, optical fiber optical tweezers crawl be positioned over it is to be detected unicellular on objective table, give and optical fiber pickup probe detect.
2. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Optical fiber optical tweezers are prepared by the following：One section of length of interception is that 2m, outer cladding diameter are 125 μm, micro-structural special type toroidal cores light
Fibre, removes coat 2cm using Miller pincers at annular core fibre two ends, alcohol wipe is dipped with non-woven fabrics totally, and use light
Fine cutter are smooth by its ends cutting, and annular core fibre one end is ground to form into 37.5 ° of inclination angles using optical fiber end grinding technique
The frustum of a cone, grinding depth is 34.5 μm, makes optical fibre ring, and annular core fibre other end and single-mode fiber are misplaced and welded,
Make single-mode fiber fibre core just to toroidal cores fiber core certain point, single-mode fiber is welded with capture light source tail optical fiber by discharging
It is connected firmly.
3. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Optical fiber pickup probe is prepared by the following：It it is 125 μm by outer cladding diameter, micro-structural special type twin-core fiber end face is cut
It is flat, it is clamped in optical fiber lapping system, white light is passed through simultaneously in two cores, is easy to position optical fiber by the CCD of grinding system
Two fibre cores；Twin-core fiber is axially rotated, straight line pushes optical fiber, make itself and grinding perpendicular to abrasive disk where making two fibre cores
Disk is ground into α=37.5 ° angle；When being ground to projected depth, upper lift optical fiber, vertically by fiber spinning 180 degree, then
The secondary optical fiber that pushes extremely is ground with abrasive disk into 37.5 ° of angles；During second of grinding, it is passed through in side fibre core has been ground
Single-frequency laser, light intensity is monitored in opposite side fibre core, when light intensity reaches maximum, stops grinding, light is in twin-core fiber end face
Pass through triple reflection at the cone angle structure ground to form, having returned to reflected light from incident fibre core collects fibre core；Pair finished will be ground
Core fibre cone angle sound end is placed upwardly on below ion sputtering instrument gold target, is coated with 500nm thick gold membranes formation reflectance coating, it is ensured that
Light can reflex to outgoing fibre core by incident fibre core in aqueous；Cone angle probe top surface gold is carefully removed with optical fiber grinding system
Film, and 50nm thick gold membranes are coated with cone angle probe top surface, it is used as surface plasma body resonant vibration film.
4. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：The dress
Put also include be used for adjust single-mode fiber I positions three-dimensional adjusting module I (3-1), for adjust single-mode fiber II positions three
Tie up adjusting module II (3-2) and the three-dimensional adjusting module III (3-3) for adjusting single-mode fiber III positions.
5. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Single-mode fiber II is 532nm, 633nm, 980nm or 1310/1550nm single-mode fiber.
6. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Coupled lens group is fiber coupling object lens, and multiple is 4 times or 10 times.
7. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Coupled lens group is waveguide fan-out-type coupled apparatus, for the beam separation of single-mode fiber I light beam and single-mode fiber II to be put down
Row is compressed to the axial symmetry fibre core spacing of micro-structural multi-core fiber I two.
8. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Micro-structural multi-core fiber I (5-1) fibre core number is even number, and fibre core is axisymmetricly distributed.
9. a kind of unicellular accurate test experience device based on optical fiber according to claim 1, it is characterised in that：It is described
Micro-structural multi-core fiber II (5-3) fibre core number is odd number, and fibre core is axisymmetricly distributed, and single-mode fiber III is by drawing cone method
Micro-structural multi-core fiber II coupling note light.
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