CN207096102U - A kind of unicellular precisely test experience device based on optical fiber - Google Patents
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Abstract
The utility model discloses a kind of unicellular precisely test experience device based on optical fiber, the device is made up of super continuous spectrums single-mode output light source and programmable optical spectrometer, detection probe is made up of micro-structural multi-core fiber, simultaneously optical fiber optical tweezers are made using micro-structural multi-core fiber, it is controlled to unicellular, under the microscope, unicellular on objective table is accurately laid on micro-structural multicore SPR probe circular cone structure detection platforms by optical tweezer.Micro-structural multi-core fiber diameter only has 125 μm, and spr sensor probe and optical tweezer is made into circular cone structure in abrading tip, suitable with unicellular tens micron dimension, can be well be used for precisely medical treatment in it is single celled precisely capture control with refractive index detect.
Description
Technical field
The utility model belongs to accurate medical research experiment equipment field, and in particular to a kind of unicellular essence based on optical fiber
Quasi- test experience device.
Background technology
With the completion of the Human Genome Project, individual human genome, Oncogenome, environmental genomics, gene sequencing
The development of technology, and biology and medical science, towards the progressively conversion of data-intensive science, " precisely medical treatment " is as biology and doctor
One novel concept in field is arisen at the historic moment, and positive guidance is provided for the more accurate and effective diagnosis of clinical disease, treatment
Effect.Precisely the implementation of medical treatment can promote medical science and technology and the further mixing together of big data and information science, so that
Medical model changes from extensive style to accurate.Therefore, domestic and international national governments and enterprise have had begun to " precisely medical treatment " back of the body
Strategy under scape is disposed with scientific research.The Chinese precisely strategic expert group of medical treatment had been set up already in Chinese Government, actively formulated " precisely
Medical treatment " strategical planning, and plan to include " 13 " major scientific and technological project.By taking malignant tumour as an example, current diagnosis mainly according to
Rely imageological examination, pathological examination and tumor markers etc., with the arrival in accurate medical epoch, unicellular control and detection
Technology is arisen at the historic moment, it has also become the study hotspot of tumour educational circles, detection of the mankind to malignant tumour is reached individual cell level.
Refractive index can be from the refractive index of environment in occupation of increasingly consequence, people as medical test parameter
Some characteristics of material are analyzed, and the refractive index of biological cell is even more then a weight of reflection light and cell interaction property
Physical quantity is wanted, is a basic parameter in many Photobiology ecsomatics experiments.Such as with spectroscopy of polarized light scattering carry out cancer,
Particularly the experimental study of early-stage cancer checkout and diagnosis when, it is necessary to which the relative index of refraction of given cell, carries out spectra inversion, ability
The shape information of cell is obtained from spectroscopic data accurate quantification.Because SPR resonance curves become to the refractive index of testing medium
Changing very sensitive, small variations in refractive index can make resonance condition that obvious change occur, can by the change for measuring resonance curve
To determine the variations in refractive index of testing medium, based on the Cleaning Principle, the technology can be applied in medical detection field.
Lung cancer is a kind of high malignant tumour of fatal rate, shows that lung cancer is sent out in Report of Chinese Cancer Control Strategy
Sick rate increases year by year, and serious threat the health of people.CT images inspection at present is to improve the Main Means of the survival rate of patient.
Show higher false positive rate and mistake be present by the result of Qualitative Imaging Diagnosis according to existing clinical imageology diagnostic result
Examine rate.The difference of scattering and absorption of the different material to light is the essence of CT images technology, and this species diversity is that light and material are mutual
Optical heterogeneity caused by effect.Macroscopic view and the biological tissue optical heterogeneity of micro-scale, corresponding main theory are different.
On molecular level and cell dimensions, electric susceptibility and refractive index are then the standard for weighing this inhomogeneities respectively.As can be seen here, it is right
The definite understanding of lung carcinoma cell refractive index is significant to the accuracy rate for improving CT images etiologic diagnosis.But now for lung
The data report of the unicellular refractive index of cancer is very few, thus it is particularly significant to carry out experimental study to its single celled refractive index.
Because most of biological tissue has stronger scattering and absorption, traditional survey based on the law of refraction to visible ray
The method for measuring transparent substance refractive index is no longer applicable.At present, mainly there is bare fibre spy to the method for Refractive Index Measurement of Bio-tissue
Pin method of testing, optical coherence tomography (OCT) method, total reflection method and refractometry is attributed to reflection using fresnel formula
Measuring method of rate etc.；Bare fibre probe method, it is the covering using sample as fiber core, by measuring " optical fiber "
Numerical aperture, so that it is determined that the refractive index of sample.Its shortcoming is, if fibre core and sample loose contact or sample size compared with
Small, this method will fail；OCT methods are to be combined together low coherence interferometer and confocal scanning imaging technology, more by filtering out
Secondary scattering light, so as to relatively accurately measure the refractive index of sample, but this method light path is complicated, is not easy to adjust.
Utility model content
In consideration of it, the utility model provides a kind of unicellular precisely test experience device based on optical fiber, on objective table
The unicellular accurate medical experiment for being captured and being detected is studied.
The purpose of this utility model realized by such technical scheme, a kind of unicellular precisely inspection based on optical fiber
Detection experiment apparatus, including super continuum source 1, coupled lens group 4, micro-structural multi-core fiber I5-1, micro-structural multi-core fiber
II5-3, spectrometer 6,980nm light sources 7, objective table 8 and electron microscope 9, the objective table are used to carry cell to be detected；Institute
State micro-structural multi-core fiber I tail ends and be provided with optical fiber pickup probe 5-2, the micro-structural multi-core fiber II tail ends are provided with
Optical fiber optical tweezers 5-4；The light injection single-mode fiber I2-1 that super continuum source 1 is sent, then the injection of coupled lens group 4 micro-structural are more
Core fibre I5-1, flashlight are redirected back into spectrum in optical fiber pickup probe 5-2 end face reflection by single-mode fiber II2-2
Instrument 6 carries out detection data processing；The light that 980nm light sources 7 are sent injects micro-structural multi-core fiber II5- by single-mode fiber III2-3
3, it is emitted and converges in optical fiber optical tweezers 5-4 end face, forms unicellular ligh trap capture field；Under the observation of electron microscope 9, optical fiber
Optical tweezer crawl be positioned over it is to be detected unicellular on objective table, give and optical fiber pickup probe detect.
Further, the device also includes being used to adjust the three-dimensional adjusting module I3-1 of single-mode fiber I positions, for adjusting list
The three-dimensional adjusting module II3-2 of the mode fiber II positions and three-dimensional adjusting module III3-3 for adjusting single-mode fiber III positions.
Further, the coupled lens group is fiber coupling object lens, and multiple is 4 times or 10 times.
Further, the coupled lens group is waveguide fan-out-type coupled apparatus, for by single-mode fiber I light beam and single mode
Optical fiber II beam separation is parallel to be compressed to the axial symmetry fibre core spacing of micro-structural multi-core fiber I two.
Further, the fibre core number of the micro-structural multi-core fiber I5-1 is even number, and fibre core is axisymmetricly distributed.
Further, the optical fiber pickup probe 5-2 is to carry out bare fibre grinding in micro-structural multi-core fiber 5-1 tail ends
Into circular cone structure and it is coated with golden film and is made.
Further, the fibre core number of the micro-structural multi-core fiber II5-3 is odd number, and fibre core is axisymmetricly distributed, single-mode optics
Fine III is by drawing cone method as micro-structural multi-core fiber II coupling note light.
Further, the optical fiber optical tweezers 5-4 is to utilize bare fibre grinding, oxyhydrogen flame in micro-structural multi-core fiber 5-3 tail ends
Cone or hydrofluoric acid corrosion are drawn into circular cone structure.
By adopting the above-described technical solution, the utility model has the following advantages：
The utility model proposes a kind of unicellular precisely test experience device based on optical fiber, in accurate medical field,
Cell control is set to reach individual cell level with highly sensitive refractive index detection.The utility model can real-time, undamaged measurement
With analyzing single celled variations in refractive index, studied so as to carry out the existence of cell with pathological condition；Its extraordinary micro-structural used
Multi-core fiber optical tweezer is less than 100 μm with unicellular SPR refractive indexes detection probe circular cone mesa diameter, exists with conventional unicellular size
Same magnitude, had broad application prospects in accurate medical research experimental field.
Brief description of the drawings
In order that the purpose of this utility model, technical scheme and advantage are clearer, below in conjunction with accompanying drawing to this practicality
It is new to be described in further detail, wherein：
Fig. 1 is overall structure composition schematic diagram of the present utility model；
Fig. 2 is the unicellular SPR refractive indexes light path schematic diagram of the utility model；
Fig. 3 is that the unicellular ligh trap of the utility model captures field light path schematic diagram.
Embodiment
Below with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail；It is it should be appreciated that it is preferred that real
Example is applied only for illustrating the utility model, rather than in order to limit the scope of protection of the utility model.
As shown in figure 1, a kind of unicellular precisely test experience device based on optical fiber, including super continuum source 1, coupling
Lens group 4, micro-structural multi-core fiber I5-1, micro-structural multi-core fiber II5-3, spectrometer 6,980nm light sources 7, objective table 8 and electricity
Sub- microscope 9, the objective table are used to carry cell to be detected；The micro-structural multi-core fiber I tail ends are provided with optical fiber biography
Feel probe 5-2, the micro-structural multi-core fiber II tail ends are provided with optical fiber optical tweezers 5-4；The light injection that super continuum source 1 is sent
Single-mode fiber I2-1, then coupled lens group 4 inject micro-structural multi-core fiber I5-1, and flashlight is in optical fiber pickup probe 5-2
End face reflection, spectrometer 6 is redirected back into by single-mode fiber II2-2 and carries out detection data processing；What 980nm light sources 7 were sent
Light injects micro-structural multi-core fiber II5-3 by single-mode fiber III2-3, is emitted and converges in optical fiber optical tweezers 5-4 end face, is formed
Unicellular ligh trap captures field；Under the observation of electron microscope 9, optical fiber optical tweezers crawl is positioned over to be detected slender on objective table
Born of the same parents, send and detected with optical fiber pickup probe.
In the present embodiment, described super continuum source 1 is NKT Photonics Superk Compact models,
Wave-length coverage 450-2400nm, general power are more than 100mW, and 0-100% is adjustable.
In the present embodiment, described single-mode fiber II is 532nm, 633nm, 980nm or 1310/1550nm single-mode optics
It is fine.
In the present embodiment, test experience device also includes being used for the three-dimensional adjusting module I3- for adjusting single-mode fiber I positions
1st, for adjusting the three-dimensional adjusting module II3-2 of single-mode fiber II positions and being adjusted for adjusting the three-dimensional of single-mode fiber III positions
Mould preparation block III3-3.Three-dimensional adjusting module I3-1 and three-dimensional adjusting module II3-2 is Thorlabs MBT602, and three-dimensional adjusts mould
Block I3-3 is Thorlabs MBT610D models.
In the present embodiment, described coupled lens group 4 can be fiber coupling object lens, and multiple is 4 times or 10 times；Also may be used
Think that waveguide is fanned out to shape coupled apparatus, for parallel to be compressed to micro-structural multi-core fiber two symmetrical by two single-mode fiber beam separations
Apart from size between core.
In the present embodiment, micro-structural multi-core fiber I5-1 cladding diameters are 125 μm, and fibre core number is even number, such as twin-core, four
Core or six core fibres, fibre core are axisymmetricly distributed, and optical fiber pickup probe 5-2 is to enter in micro-structural multi-core fiber I5-1 tail ends
Row bare fibre is ground into circular cone structure and is coated with 50nm golden films and is made；Micro-structural multi-core fiber II5-3 cladding diameters are 125 μ
M, fibre core number are odd number, and such as three cores, five cores or annular core fibre, fibre core are axisymmetricly distributed, and single-mode fiber III2-3 passes through drawing
Cone method is its coupling note light；Optical fiber optical tweezers 5-4 is to utilize bare fibre grinding, oxyhydrogen flame in micro-structural multi-core fiber II5-3 tail ends
Cone or hydrofluoric acid corrosion is drawn to make multi-beam converge to form unicellular ligh trap capture field into truncated cone shape.
The making to unicellular precisely test experience device building block is described further below.
1st, unicellular capture optical fiber optical tweezers are made：One section of length of interception is 2m, outer cladding diameter is 125 μm, and micro-structural is extraordinary
Annular core fibre, coat 2cm is removed using Miller pincers at its both ends, it is clean to dip alcohol wipe with non-woven fabrics, and uses light
Fine cutter are smooth by its ends cutting, and optical fiber one end is ground into the frustum of a cone at 37.5 ° of inclination angles using optical fiber end grinding technique,
It is 34.5 μm to grind depth (i.e. frustum of a cone height), the toroidal cores optical fiber optical tweezers structure to complete and its outgoing light field such as Fig. 3
It is shown.Optical fibre ring other end and single-mode fiber dislocation are welded, make single-mode fiber fibre core face toroidal cores fiber core
A bit, single-mode fiber is welded to connect firmly with capture light source tail optical fiber by electric discharge.
2nd, optical fiber pickup probe is made：It is 125 μm by outer cladding diameter, micro-structural special type twin-core fiber end face is cut flat with,
It is clamped in optical fiber lapping system, white light is passed through simultaneously in two cores, is easy to position two of optical fiber by the CCD of grinding system
Fibre core.Twin-core fiber is axially rotated, straight line where two fibre cores is pushed optical fiber perpendicular to abrasive disk, makes it with abrasive disk into α
=37.5 ° of angles, are ground.When being ground to projected depth, upper lift optical fiber, vertically by fiber spinning 180 degree, push again
Optical fiber is extremely ground with abrasive disk into 37.5 ° of angles.Second when grinding, and being passed through single-frequency in side fibre core has been ground swashs
Light, light intensity is monitored in opposite side fibre core, when light intensity reaches maximum, stop grinding, light twin-core fiber end surface grinding into
Cone angle structure at pass through triple reflection, from incident fibre core returned to reflected light collect fibre core.The twin-core fiber finished will be ground
Cone angle sound end is placed upwardly on below ion sputtering instrument gold target, is coated with 500nm thick gold membranes and is formed reflectance coating, ensures water-soluble
Light can reflex to outgoing fibre core by incident fibre core in liquid.Cone angle probe top surface golden film is carefully removed with optical fiber grinding system, and
50nm thick gold membranes are coated with cone angle probe top surface, as surface plasma body resonant vibration film.
3rd, experimental provision integral manufacturing：Two root longs about 1m single-mode fiber is taken, both ends remove coat using Miller pincers
2cm, it is clean and using optical fiber cutter that its ends cutting is smooth to dip alcohol wipe with non-woven fabrics；Optical fiber after handling well
After being clamped with naked fibre adapter, a connection light source, a connection spectrometer, by two single-mode fiber other ends and twin-core light
Fine planar end surface one end is placed in precision three-dimensional adjustment frame, by coupled lens group be fixed on single-mode fiber and twin-core fiber it
Between, adjusted by calibrating, realize paths effect as shown in Figure 2, after spectrometer is preferably received detection
Flashlight.
4th, device operates：The optical fiber pickup probe that twin-core fiber is made contains cell as on slide, taking on a small quantity
Nutrient solution be added dropwise on probe, submerge optical fiber pickup probe, then by toroidal cores optical fiber optical tweezers with precision three-dimensional adjust mould
The micro- behaviour of block is moved under environmental liquids liquid level, under electron microscope auxiliary, makes optical fiber optical tweezers confocal flat with optical fiber pickup probe
Face, and in the case where micro- behaviour manipulates, capture individual cells with optical fiber optical tweezers, reclined to optical fiber pickup probe end face sensing unit,
Light source and spectrometer are opened, after system is stable, sensing spectral line is obtained by spectrometer.
The utility model proposes a kind of unicellular precisely test experience device based on optical fiber, detection device is by super continuous spectrums
Single-mode output light source is formed with programmable optical spectrometer, and detection probe is made up of micro-structural multi-core fiber, while more using micro-structural
Core fibre makes optical fiber optical tweezers, is controlled to unicellular, under the microscope, will be unicellular accurate by optical tweezer on objective table
It is laid on micro-structural multicore SPR probe circular cone structure detection platforms.Micro-structural multi-core fiber diameter only has 125 μm, tip
It is ground into circular cone structure and spr sensor probe and optical tweezer is made, use that can good suitable with unicellular tens micron dimension
Single celled precisely capture control is detected with refractive index in accurate medical treatment.
Preferred embodiment of the present utility model is the foregoing is only, is not limited to the utility model, it is clear that this area
Technical staff can to the utility model carry out it is various change and modification without departing from spirit and scope of the present utility model.This
Sample, if these modifications and variations of the present utility model belong to the utility model claims and its equivalent technologies scope it
Interior, then the utility model is also intended to comprising including these changes and modification.
Claims (8)
- A kind of 1. unicellular precisely test experience device based on optical fiber, it is characterised in that：Including super continuum source (1), coupling Close lens group (4), micro-structural multi-core fiber I (5-1), micro-structural multi-core fiber II (5-3), spectrometer (6), 980nm light sources (7), objective table (8) and electron microscope (9), the objective table are used to carry cell to be detected；The micro-structural multi-core fiber I Tail end is provided with optical fiber pickup probe (5-2), and the micro-structural multi-core fiber II tail ends are provided with optical fiber optical tweezers (5-4)；It is super The light injection single-mode fiber I (2-1) that continuous spectrum light source (1) is sent, then coupled lens group (4) injection micro-structural multi-core fiber I (5-1), flashlight are redirected back into spectrum in optical fiber pickup probe (5-2) end face reflection by single-mode fiber II (2-2) Instrument (6) carries out detection data processing；The light that 980nm light sources (7) are sent injects micro-structural multi-core optical by single-mode fiber III (2-3) Fine II (5-3), it is emitted and converges in the end face of optical fiber optical tweezers (5-4), forms unicellular ligh trap capture field；In electron microscope (9) observe under, optical fiber optical tweezers crawl be positioned over it is to be detected unicellular on objective table, give and optical fiber pickup probe detect.
- A kind of 2. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：The dress Put also include be used for adjust single-mode fiber I positions three-dimensional adjusting module I (3-1), for adjust single-mode fiber II positions three Tie up adjusting module II (3-2) and the three-dimensional adjusting module III (3-3) for adjusting single-mode fiber III positions.
- A kind of 3. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：It is described Coupled lens group is fiber coupling object lens, and multiple is 4 times or 10 times.
- A kind of 4. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：It is described Coupled lens group is waveguide fan-out-type coupled apparatus, for the beam separation of single-mode fiber I light beam and single-mode fiber II to be put down Row is compressed to the axial symmetry fibre core spacing of micro-structural multi-core fiber I two.
- A kind of 5. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：It is described Micro-structural multi-core fiber I (5-1) fibre core number is even number, and fibre core is axisymmetricly distributed.
- A kind of 6. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：It is described Optical fiber pickup probe (5-2) is to carry out bare fibre in micro-structural multi-core fiber I (5-1) tail end to be ground into circular cone structure simultaneously Golden film is coated with to be made.
- A kind of 7. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：It is described Micro-structural multi-core fiber II (5-3) fibre core number is odd number, and fibre core is axisymmetricly distributed, and single-mode fiber III is by drawing cone method Micro-structural multi-core fiber II coupling note light.
- A kind of 8. unicellular precisely test experience device based on optical fiber according to claim 1, it is characterised in that：It is described Optical fiber optical tweezers (5-4) are to draw cone or hydrofluoric acid using bare fibre grinding, oxyhydrogen flame in micro-structural multi-core fiber II (5-3) tail end Corrode into circular cone structure.
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| CN201721085621.XU CN207096102U (en) | 2017-08-28 | 2017-08-28 | A kind of unicellular precisely test experience device based on optical fiber |
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| CN201721085621.XU CN207096102U (en) | 2017-08-28 | 2017-08-28 | A kind of unicellular precisely test experience device based on optical fiber |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107300538A (en) * | 2017-08-28 | 2017-10-27 | 重庆三峡医药高等专科学校 | A kind of unicellular accurate test experience device based on optical fiber |
| CN108703138A (en) * | 2018-07-08 | 2018-10-26 | 苏州美丽澄电子技术有限公司 | A kind of method and device freezed in optical tweezer cell and particle to liquid nitrogen |
| CN109540179A (en) * | 2018-12-21 | 2019-03-29 | 南京信息工程大学 | Optical fiber taper sensing probe based on surface plasma body resonant vibration and preparation method thereof |
| CN110071417A (en) * | 2019-05-14 | 2019-07-30 | 桂林电子科技大学 | Three core fibre cell laser of coaxial Crossed Circle with stretch function |
-
2017
- 2017-08-28 CN CN201721085621.XU patent/CN207096102U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107300538A (en) * | 2017-08-28 | 2017-10-27 | 重庆三峡医药高等专科学校 | A kind of unicellular accurate test experience device based on optical fiber |
| CN108703138A (en) * | 2018-07-08 | 2018-10-26 | 苏州美丽澄电子技术有限公司 | A kind of method and device freezed in optical tweezer cell and particle to liquid nitrogen |
| CN109540179A (en) * | 2018-12-21 | 2019-03-29 | 南京信息工程大学 | Optical fiber taper sensing probe based on surface plasma body resonant vibration and preparation method thereof |
| CN109540179B (en) * | 2018-12-21 | 2024-05-17 | 南京信息工程大学 | Optical fiber conical sensing probe based on surface plasma resonance and manufacturing method thereof |
| CN110071417A (en) * | 2019-05-14 | 2019-07-30 | 桂林电子科技大学 | Three core fibre cell laser of coaxial Crossed Circle with stretch function |
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