CN207067546U - A kind of step multimode fibre optical tweezer based on skew ray annular light field - Google Patents

A kind of step multimode fibre optical tweezer based on skew ray annular light field Download PDF

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Publication number
CN207067546U
CN207067546U CN201721083580.0U CN201721083580U CN207067546U CN 207067546 U CN207067546 U CN 207067546U CN 201721083580 U CN201721083580 U CN 201721083580U CN 207067546 U CN207067546 U CN 207067546U
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China
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multimode fibre
step multimode
light field
oblique incidence
skew ray
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CN201721083580.0U
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Chinese (zh)
Inventor
张永慧
苏于东
魏勇
刘春兰
姜友嫦
许刚
岳西
刘奉
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Chongqing Three Gorges Medical College
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Chongqing Three Gorges Medical College
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Abstract

The utility model discloses a kind of step multimode fibre optical tweezer based on skew ray annular light field, including light source (1), single-mode fiber (2), step multimode fibre (4) and capture tweezer head (6);In light source optical coupling injection single-mode fiber, single-mode fiber notes light to the oblique incidence of step multimode fibre, light spiral onwards transmission and is formed annular light field in the form of skew ray in step multimode fibre, and circular cone structure of the ring light in step multimode fibre end face is totally reflected convergence, produces strong trapping stiffness.The device can capture a unicellular and grade microlayer model of ascending to heaven, and change note angular, position, spacing by oblique incidence adjusting means, realize that the accurate capture to cell, carrying, ejection, screening etc. operate.

Description

A kind of step multimode fibre optical tweezer based on skew ray annular light field
Technical field
The utility model belongs to accurate medical research experiment equipment field, and in particular to one kind is based on skew ray annular light field Step multimode fibre optical tweezer.
Background technology
In light field most strength, i.e., optical tweezer is using the gradient force that distribution of light intensity spatial variations are formed the stable capture of particulate The focal position of light beam.When laser beam moves, can drives particulate to move together, realizes and the precision of particulate is manipulated.Cell It is the base unit of life 26S Proteasome Structure and Function, optical tweezer is used to conform exactly to cell, Asia carefully the characteristics of manipulating particulate in micrometer range The research of born of the same parents' level.In the contention that 20 end of the centurys various new technologies are vied with each other for glamour, optical tweezer studies living cells with its dynamic Unique function, show credit human eye purpose brilliance radiance.Traditional optical tweezer is built based on optical microscope system, and it is logical Micro objective is crossed by laser beam focusing, ligh trap is formed using the gradient field of force near focusing center, realizes and particulate is caught Obtain and manipulate.Traditional optical tweezer is complicated, bulky, expensive, and operative skill requires high, and sample one-movement-freedom-degree is small, Limit it and be widely applied value.Compared with based on microscopical conventional optical optical tweezers system, the miniature probe formula light based on single fiber Tweezers system is simple in construction, compact, and it is big to catch sunken scope, manoeuvrable, is conveniently adjusted, is adapted to more biological cells and biology The light microoperation demand of molecule, application of the micro- manipulation technology of laser in life science is expanded, is had a wide range of applications Prospect.
Utility model content
In consideration of it, the utility model provides a kind of step multimode fibre optical tweezer based on skew ray annular light field.The device A unicellular and grade microlayer model of ascending to heaven can be captured, note angular, position, spacing are changed by oblique incidence adjusting means, realized to thin The operation such as the accurate capture of born of the same parents, carrying, ejection, screening.
The purpose of this utility model realized by such technical scheme, a kind of rank based on skew ray annular light field Jump multimode fibre optical tweezer, including light source 1, single-mode fiber 2 and step multimode fibre 4, and the end of step multimode fibre sets capture Tweezer first 6;In light source optical coupling injection single-mode fiber, single-mode fiber notes light, step multimode fibre to the oblique incidence of step multimode fibre Middle light spiral onwards transmission and is formed annular light field in the form of skew ray, and ring light converges in the first 6 end face total reflection of capture tweezer It is poly-, produce strong trapping stiffness;Also include oblique incidence adjusting means I3-1 and oblique incidence adjusting means II3-2, the oblique incidence regulation The horizontal clamping step multimode fibres of device II, the oblique orientation clamping single-mode fibers of oblique incidence adjusting means I.
Further, the X-axis that the oblique incidence adjusting means I includes being used to adjust single-mode fiber position on horizontal level is adjusted Save part I, adjust part I and for adjusting single-mode fiber emergent light for adjusting the Y-axis of single-mode fiber position on vertical position The rotating disk of shooting angle rotates regulation part I.
Further, the incident section that the oblique incidence adjusting means II includes being used to adjust step multimode fibre is in horizontal level The X-axis regulation part II of upper position, the Y-axis regulation part of incident section position on vertical position for adjusting step multimode fibre The II and rotating disk rotation regulation part II for adjusting step multimode fibre incidence section angle.
Further, the capture tweezer head is using bare fibre grinding technique, and system is processed to step multimode fibre tail end Into passing sequentially through that coat is divested, cut, grinding, step multimode fibre tail end is processed into the symmetrical frustum of a cone by polishing step Structure.
Further, the optical fiber optical tweezers also include micro- behaviour's hand 5, and micro- behaviour's hand is used for the position for adjusting multimode step optical fiber latter end Put.
By adopting the above-described technical solution, the utility model has the following advantages:
It is fine in multimode fibre the utility model proposes a kind of step multimode fibre optical tweezer based on skew ray annular light field Using the meridional ray in the method elimination fibre core of oblique incidence in core, the annular light field being made up of completely skew ray is constructed, is used After bare fibre grinding technique processes circular cone structure in multimode fibre end face, by the ring of the skew ray transmitted in multimode fibre composition After the convergence of shape light field, intensity gradient field is produced, is configured to strong trapping stiffness single fiber optical tweezers, can capture unicellular, can also captured Ascend to heaven a grade microlayer model;Note angular, position, spacing are changed by oblique incidence adjusting means, can effectively change optical tweezer emergent light The distribution of field and light intensity etc., so as to adjust capture point position and force trapping, and then utilize cheap step-index multimode Optical fiber, realize that the accurate capture to cell, carrying, ejection, screening etc. operate.The utility model proposes skew ray wire loop square Multimode fibre optical tweezer, force trapping is big, and catch position is adjustable, the screening of microcosmic particle, move, store and carrying in terms of there is pole High application value, had broad application prospects in accurate medical research experimental field.
Brief description of the drawings
In order that the purpose of this utility model, technical scheme and advantage are clearer, below in conjunction with accompanying drawing to this practicality It is new to be described in further detail, wherein:
Fig. 1 is overall structure composition schematic diagram of the present utility model;
Fig. 2 is that skew ray transmits schematic diagram in a fiber;
Fig. 3 is that skew ray is projected in fiber end face schematic diagram;
Fig. 4 is skew ray annular light field step multimode fibre optical tweezer light field mechanism figure.
Embodiment
Below with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail;It is it should be appreciated that it is preferred that real Example is applied only for illustrating the utility model, rather than in order to limit the scope of protection of the utility model.
A kind of step multimode fibre optical tweezer based on skew ray annular light field, including light source 1, single-mode fiber 2, oblique incidence tune Regulating device I3, oblique incidence adjusting means II3-2, step multimode fibre 4, for adjust step multimode fibre outgoing fragment position it is micro- Hand 5, microscope 7 are grasped, the end of step multimode fibre sets capture tweezer first 6;Light source optical coupling is by being installed in single module optical fiber In the optical coupling lens injection single-mode fiber of front end, make single-mode fiber oblique to step multimode fibre with oblique incidence adjusting means I Penetrate note light, light spiral onwards transmission and is formed annular light field in the form of skew ray in step multimode fibre, and ring light is being caught The first 6 end face total reflection convergence of tweezer is obtained, strong trapping stiffness is produced, a unicellular and grade microlayer model of ascending to heaven can be captured, step multimode fibre The position of incident section by oblique incidence adjusting means II control, by oblique incidence adjusting means I, II change note angular, position, Away from, it can effectively change optical tweezer and be emitted distribution and light intensity of light field etc., the regulation capture under the auxiliary of microscope and micro- behaviour's hand 5 Point position and force trapping, and then cheap step-index multimode fiber is utilized, realize the accurate capture to cell, remove The operations such as fortune, ejection, screening.
In the present embodiment, described light source 1 is 980nm lasers, and it uses butterfly semiconductor, is protected with reference to optical fiber FBG It is stable to demonstrate,prove spectral wavelength, power is continuously adjustable, and after 980nm single-mode fibers, peak power output is more than 400mW.
In the present embodiment, described single-mode fiber 2 is 980nm single-mode fibers, and numerical aperture 0.2,980nm corresponds to mould field 4.2 μm of diameter, 3.6 μm of core diameter, 125 μm of cladding diameter, front end are coupled by coupled lens with 980nm light sources 1.
In the present embodiment, described oblique incidence adjusting means I, II is that two accurate adjustment frames form, each adjustment frame It is respectively provided with X-axis, Y-axis displacement regulating platform and rotating disk and rotates regulation part, the capillary tubing that may be inserted into optical fiber is fixed with rotating disk And fiber stub, the horizontal clamping multimode fibres 4 of wherein oblique incidence adjusting means II, the oblique orientation clampings of oblique incidence adjusting means I are single Mode fiber 2, adjusted by the cooperation of both sides oblique incidence adjusting means, it is oblique to step multimode fibre can effectively to change single-mode fiber Note angular, the position and spacing of incidence note light, realize that the accurate capture to cell, carrying, screening etc. operate;When regulation single mode When optical fiber is with step multimode fibre end face face, step multimode fibre outgoing light field only has thrust, completes the ejection to cell.
In the present embodiment, described step multimode fibre 4 is glass-clad step-index multimode fiber, numerical aperture 0.22,105 μm of optical fiber core diameter, 125 μm of cladding diameter.
In the present embodiment, described capture tweezer first 6 is using bare fibre grinding technique, to the tail end of step multimode fibre 4 It is processed and is made, passes sequentially through coat and the step such as divest, cut, grinding, polishing and be processed into the tail end of multimode fibre 4 symmetrically Circular cone structure.
The making to unicellular precisely detection means building block is described further below.
1st, tweezer head manufacturing process is captured:As shown in figure 1, one section of length of interception is 2m, outer cladding diameter is 125 μm, fibre core is straight The step-index multimode fiber that 105 μm of footpath, coat 2cm is removed using Miller pincers at its both ends, alcohol is dipped with non-woven fabrics Wiped clean, and it is using optical fiber cutter that its ends cutting is smooth, the step-index multimode fiber cut flat with is installed in light On fine grinding system, optical fiber is pushed, it, into 37.5 ° of inclination angle, optical fiber rotation synchronization is carried out with 8000 mesh sand paper with abrasive disk Grinding.When grinding depth to 34.5 μm, upper lift optical fiber, change 12000 mesh sand paper polishing optical fiber and grind face 2 hours, ensure light Fine inclined-plane is substantially flat;980nm laser device lasers are injected into single-mode fiber, light is noted in single-mode fiber oblique incidence to multimode fibre, tiltedly Incident method eliminates the meridional ray in fibre core, as shown in Figure 2;Construct the annular light field being made up of completely skew ray, such as Fig. 3 Shown, the annular light field of the skew ray composition transmitted in multimode fibre produces light intensity after circular cone structure reflecting focal is ground Gradient fields, strong trapping stiffness single fiber optical tweezers are configured to, can capture unicellular, a grade microlayer model of ascending to heaven can be also captured, such as Fig. 4 institutes Show.
2nd, oblique incidence adjusting means operates:Long 2m 980nm single-mode fibers are intercepted, clamping peeling optical fibre both ends using Miller applies Each 2cm of coating, fibre cladding is cleaned up with alcohol, both ends of the surface are cut to smooth, tail end installation coupling with optical fiber cutter Lens couple with 980nm light sources 1, capillary tubing and fiber stub on other end insertion oblique incidence adjusting means I rotating disks;It will grind Capillary tubing and optical fiber on the non-abrasive tip insertion oblique incidence adjusting means II rotating disks of step-index multimode fiber of milled are inserted Core, and it is horizontal positioned;Oblique incidence adjusting means I single-mode fibers X-axis and Y-axis regulating platform, and rotary turnplate are adjusted, is allowed to oblique incidence Into step multimode fibre, adjusted by the cooperation of both sides adjustment frame, can effectively change single-mode fiber to step multimode fibre Note angular, the position and spacing of light are noted in oblique incidence, realize that the accurate capture to cell, carrying, screening etc. operate.
3rd, experimental provision is overall:In 980nm light sources optical coupling injection single-mode fiber, make list with oblique incidence adjusting means I, II Mode fiber gives step multimode fibre oblique incidence note light, and spiral onwards transmission is simultaneously in the form of skew ray for light in step multimode fibre Annular light field is formed, circular cone structure of the ring light in step multimode fibre end face is totally reflected convergence, produces strong trapping stiffness, can catch Obtain a unicellular and grade microlayer model of ascending to heaven;Note angular, position, spacing are changed by oblique incidence adjusting means I, II, can be effective Change distribution and light intensity of optical tweezer outgoing light field etc., so as to adjust capture point position and force trapping, and then utilize cheap Step-index multimode fiber, realize that the accurate capture to cell, carrying, ejection, screening etc. operate.It can observe under the microscope Operational circumstances of the optical tweezer to cell.
The utility model proposes a kind of step multimode fibre optical tweezer based on skew ray annular light field, given by single-mode fiber Light is noted in multimode fibre oblique incidence, light spiral onwards transmission in the form of skew ray in multimode fibre, and in multimode fibre fibre core End face forms annular light field, using bare fibre grinding technique in multimode fibre end surface grinding circular cone structure, multimode fibre fibre core In annular light field be totally reflected and converge in fiber end face circular cone structure, convergence focus area produces strong trapping stiffness, can capture winged Upgrade microlayer model and unicellular, for unicellular accurate control and unicellular micro-administration.Adjusted by the cooperation of both sides adjustment frame Section, it can effectively change note angular from light to multimode fibre oblique incidence, position and spacing that single-mode fiber notes, for cell sieve Choosing;When adjusting single-mode fiber with multimode fibre end face face, multimode fibre outgoing light field only has thrust, completes the bullet to cell Penetrate.
Preferred embodiment of the present utility model is the foregoing is only, is not limited to the utility model, it is clear that this area Technical staff can to the utility model carry out it is various change and modification without departing from spirit and scope of the present utility model.This Sample, if these modifications and variations of the present utility model belong to the utility model claims and its equivalent technologies scope it Interior, then the utility model is also intended to comprising including these changes and modification.

Claims (5)

  1. A kind of 1. step multimode fibre optical tweezer based on skew ray annular light field, it is characterised in that:Including light source (1), single-mode optics Fine (2) and step multimode fibre (4), the end of step multimode fibre set capture tweezer head (6);Light source optical coupling injects single-mode optics In fibre, single-mode fiber gives step multimode fibre oblique incidence note light, in step multimode fibre light in the form of skew ray spiral to Preceding to transmit and form annular light field, ring light converges in the total reflection of capture tweezer head (6) end face, produces strong trapping stiffness;Also include oblique Incident adjusting means I (3-1) and oblique incidence adjusting means II (3-2), the horizontal clamping steps of the oblique incidence adjusting means II are more Mode fiber, the oblique orientation clamping single-mode fibers of oblique incidence adjusting means I.
  2. A kind of 2. step multimode fibre optical tweezer based on skew ray annular light field according to claim 1, it is characterised in that: The X-axis that the oblique incidence adjusting means I includes being used to adjust single-mode fiber position on horizontal level adjusts part I, for adjusting The Y-axis regulation part I of whole single-mode fiber position on vertical position and the rotating disk for adjusting single-mode fiber emergent light shooting angle Rotate regulation part I.
  3. A kind of 3. step multimode fibre optical tweezer based on skew ray annular light field according to claim 2, it is characterised in that: The oblique incidence adjusting means II includes being used to adjust the X-axis regulation of incident section position on horizontal level of step multimode fibre Part II, the Y-axis regulation part II of incident section position on vertical position for adjusting step multimode fibre and for adjusting rank The rotating disk for the multimode fibre incidence section angle that jumps rotates regulation part II.
  4. A kind of 4. step multimode fibre optical tweezer based on skew ray annular light field according to claim 1, it is characterised in that: The capture tweezer head is using bare fibre grinding technique, and step multimode fibre tail end is processed and is made, passes sequentially through coating Layer is divested, cut, grinding, step multimode fibre tail end is processed into symmetrical circular cone structure by polishing step.
  5. A kind of 5. step multimode fibre optical tweezer based on skew ray annular light field according to claim 1, it is characterised in that: The optical fiber optical tweezers also include micro- behaviour's hand (5), and micro- behaviour's hand is used for the position for adjusting multimode step optical fiber latter end.
CN201721083580.0U 2017-08-28 2017-08-28 A kind of step multimode fibre optical tweezer based on skew ray annular light field Expired - Fee Related CN207067546U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107357044A (en) * 2017-08-28 2017-11-17 重庆三峡医药高等专科学校 A kind of step multimode fibre optical tweezer based on skew ray annular light field
CN110993140A (en) * 2019-11-15 2020-04-10 桂林电子科技大学 Single-core optical fiber-multi-core optical fiber combined optical motor system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107357044A (en) * 2017-08-28 2017-11-17 重庆三峡医药高等专科学校 A kind of step multimode fibre optical tweezer based on skew ray annular light field
CN110993140A (en) * 2019-11-15 2020-04-10 桂林电子科技大学 Single-core optical fiber-multi-core optical fiber combined optical motor system

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Granted publication date: 20180302