CN112043740A - 一种太子参须提取物在制备抑制鸭h9n2亚型禽流感病毒药物中的应用 - Google Patents
一种太子参须提取物在制备抑制鸭h9n2亚型禽流感病毒药物中的应用 Download PDFInfo
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Abstract
本发明属于中药应用技术领域，具体公开一种太子参须提取物在制备抑制鸭H9N2亚型禽流感病毒药物中的应用。本发明首次发现太子参须提取物对鸭H9N2亚型禽流感病毒在MDCK细胞上的体外增殖具有一定抑制作用，且呈相应的剂量关系，为探究太子参须在AIV疫苗佐剂的开发提供一定的基础。
Description
技术领域
本发明属于中药应用技术领域，具体涉及一种太子参须提取物在制备抑制鸭H9N2亚型禽流感病毒药物中的应用。
背景技术
H9N2亚型禽流感病毒（AIV）起源于野鸟与水禽，自1966年首次分离后便开启全球化的蔓延与爆发，禽流感属禽类接触性传染病，随着现代养禽集约化与规模化的推进，H9N2亚型 AIV已成制约养禽业健康可持续发展的重要因素之一。目前，H9N2亚型 AIV在国内家禽中分布广泛，且在新型禽流感的演化中角色重要，而当前疫苗接种是公认的控制AIV流行的最有效方法。
随着国内养殖业 “减抗”、“无抗”模式的推行，中草药及其提取物已引起行业专家和学者的足够重视。于潼等人研究发现黄芪多糖纯品与灭活H9N2亚型禽流感病毒混合时具有协同作用，可提高小鼠外周血B淋巴细胞和CD3+CD4+T淋巴细胞亚群比例，并对小鼠外周血T淋巴细胞亚群有调节作用；黄劲等人研究发现野菊花、穿心莲、柴胡等10味中药提取的中药制剂对H9N2亚型 AIV攻毒鸡具有有效抵抗效力，减少发病率和死亡率，但截止目前，经文献检索未见有针对植物根须部位组分具有抑制鸭H9N2亚型禽流感病毒的作用报道，更未见太子参植株体及其根须具有类似或相似的功能研究结果。
发明内容
为克服现有技术中存在的不足之处，本发明的目的旨在提供一种太子参须提取物在制备抑制鸭H9N2亚型禽流感病毒药物中的应用。
为实现上述目的，本发明采取的技术方案如下：
一种太子参须提取物在制备抑制鸭H9N2亚型禽流感病毒药物中的应用。
所述太子参须提取物可按现有技术制备获得或通过市购获得，较好地，所述太子参须提取物优选按照中国专利CN 201610758305.8中公开的制备方法获得。
有益效果：本发明首次发现太子参须提取物对鸭H9N2亚型禽流感病毒在MDCK细胞上的体外增殖具有一定抑制作用，且呈相应的剂量关系，为探究太子参须在AIV疫苗佐剂的开发提供一定的基础。
具体实施方式
以下结合具体实施例，对本发明做进一步说明。应理解，以下实施例仅用于说明本发明而非用于限制本发明的范围。
实施例1
太子参须提取物在对抑制鸭H9N2亚型禽流感病毒的抑制实验：
1 材料和方法：
1.1 材料
太子参须提取物干粉：按照中国专利CN 201610758305.8中实施例1公开的制备方法获得太子参须提取液，以g/mL计，向子参须提取液中加入6%的可溶性淀粉，搅拌混匀，然后经喷雾干燥获得太子参须提取物干粉。
鸭H9N2亚型禽流感病毒和MDCK细胞（犬肾传代细胞）均保藏于福建省农业科学院畜牧兽医研究所。
实验方法
实验中，鸭H9N2亚型禽流感病毒的扩增与MDCK细胞的培养均参考文献1：张彬. 鸭H9N2亚型禽流感病毒在鹧鸪和鸡体内复制能力的比较[D]. 南宁：广西医科大学, 2016.；鸭H9N2亚型禽流感病毒毒力检测方法采用文献1公开的TCID50实验法。
实验分为太子参须提取物高、低剂量组和空白对照组，不同组别条件下使得103 TCID50 鸭H9N2亚型禽流感病毒进行感染MDCK细胞，具体用药剂量如下：A、太子参须提取物低剂量组每孔0.1g/3mL（每孔添加3mL MDCK细胞培养液+0.1g的太子参须提取物干粉）；B、太子参须提取物高剂量组每孔0.25g/3mL（每孔添加3mL MDCK细胞培养液+0.25g的太子参须提取物干粉）；C、空白对照组采用每孔3mL生理盐水；每组3个复孔，48 h后测定后感染孔TCID50值，结果用平均值±标准差值（M±S）表示，数据均采用SPSS统计处理软件进行单因素方差分析。
实验结果
实验结果见表1。由表1可知，较空白对照组，太子参须提取物对鸭H9N2亚型禽流感病毒在MDCK细胞上的体外增殖具有一定抑制作用，且太子参须提取物高剂量组的抑制作用最优，统计学差异极显著（P＜0.01）；同时对比太子参须提取物高低剂量组的H9N2亚型禽流感病毒Log10 TCID50值，可知：太子参须提取物对鸭H9N2亚型禽流感病毒体外生长的抑制作用呈一定的剂量关系。
结论与讨论
实验中采用TCID50实验法，TCID50可检测病毒毒力大小，比较空白对照组和药物处理组的TCID50值可利于直观判断鸭H9N2亚型禽流感病毒在MDCK细胞上的体外增殖情况和药物抑制效果，结果发现：太子参须提取物对鸭H9N2亚型禽流感病毒在MDCK细胞上的体外增殖具有一定抑制作用，且呈相应的剂量关系。
Claims (2)
1.一种太子参须提取物在制备抑制鸭H9N2亚型禽流感病毒药物中的应用。
2.如权利要求1所述的太子参须提取物在制备抑制鸭H9N2亚型禽流感病毒药物中的应用，其特征在于：所述太子参须提取物按照中国专利CN 201610758305.8中公开的制备方法获得。
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| CN114699520A (zh) * | 2022-04-14 | 2022-07-05 | 福建贝迪药业有限公司 | 一种太子参须提取物作为禽1型副黏病毒疫苗佐剂的应用 |
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| US20110195135A1 (en) * | 2008-10-08 | 2011-08-11 | Pokka Corporation | Anti-avian influenza virus agent, and product containing anti-avian influenza virus agent |
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