CN112021173A - Rapid breeding method of pakchoi Ogura cytoplasmic male sterile line - Google Patents
Rapid breeding method of pakchoi Ogura cytoplasmic male sterile line Download PDFInfo
- Publication number
- CN112021173A CN112021173A CN201910482461.XA CN201910482461A CN112021173A CN 112021173 A CN112021173 A CN 112021173A CN 201910482461 A CN201910482461 A CN 201910482461A CN 112021173 A CN112021173 A CN 112021173A
- Authority
- CN
- China
- Prior art keywords
- seeds
- months
- line
- chinese cabbage
- pakchoi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a breeding method of an Ogrua cytoplasmic male sterile line of Brassica napobrassica, which comprises the following steps: 1) hybridizing by using Ogrua cytoplasmic male sterile P0523A as female parent and using Brassica rapa excellent inbred line WS-28-1 as male parent to obtain F1(ii) a 2) In the same year, the month of 5 is F1Using self-bred line WS-28-1 as female parent and recurrent male parent, vernalizing seed, sowing in greenhouse, and harvesting Chinese cabbage BC in the same year and 8 months1(ii) a 3) Harvesting Chinese cabbage BC in the same year in 8 months1Vernalizing with self-bred WS-28-1 seed to obtain Chinese cabbage seed BC in the same year and 12 months2(ii) a 4) Sowing in the same year in 12 months, and using Chinese cabbage seed BC2Backcrossing with selfing line WS-28-1 as male parent to obtain Chinese cabbage seed BC 5 months in the next year3(ii) a 5) In the next 5 months, Chinese cabbage seeds BC3Taking the 'WS-28-1' variety of the inbred line as a male parent, performing low-temperature vernalization on the male parent and the female parent, and repeating the step 2). 8 months in the second yearTo obtain the sterile line CMS _28-1A similar to the Chinese cabbage WS-28-1. The invention can be used for quickly creating the Ogura cytoplasmic male sterile line of the Brassica napobrassica.
Description
Technical Field
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method of an Ogrua cytoplasmic male sterile line of Brassica napobrassica, which is particularly suitable for utilizing heterosis of Brassica rapa.
Background
Yellow pakchoi (Brassica campestris L.ssp. chinensis-sis Makino var. communis Tsen et Lee cv.Aijiaohuang) is a local cabbage variety native to the suburb of Nanjing. The Chinese cabbage is called short foot yellow because of short stem, white stem and yellow heart. As an amphoteric cross-pollinated plant, the Chinese cabbage has remarkable heterosis. Because the floral organs are small, the single flower has few knots and the seeding amount per unit area is larger, in order to utilize the heterosis, the problem of hybrid seed production means must be solved firstly. The use of cytoplasmic male sterile line is an ideal way to prepare hybrid species.
Chinese cabbage is a biennial plant, low-temperature vernalization and long-day induction are required for the conversion of vegetative growth into reproductive growth, and sexual propagation of only one generation can be completed each year under natural conditions. Under normal conditions, only 1 generation can be propagated in 1 year, however, in the transformation process of the male sterile line, breeding links such as hybridization and backcross are needed, and 5-6 generations are generally needed to obtain homozygous breeding materials. Thereby influencing the progress of breeding work and needing generation-added propagation for accelerating the breeding speed of the Chinese cabbage variety.
Disclosure of Invention
The invention relates to a rapid breeding method of Ogura cytoplasmic male sterile line of Brassica napobrassica by using Ogrua cytoplasmic male sterile line as a sterile source and transferring cytoplasmic sterile gene into Brassica napobrassica to create Brassica napobrassica cytoplasmic gene male sterile line. The method is easy to implement and simple and convenient to operate, and the Ogura cytoplasmic male sterile line of the short-foot yellow pakchoi can be quickly created by using the method, and has the advantages of insensitivity to temperature, short-term response to illumination, no pollen and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
the technical conception is as follows: in order to better utilize the heterosis of the cabbage vegetable crops and create a cytoplasmic male sterile line to solve the problem of hybrid seed production, the invention uses the Ogrua cytoplasmic male sterile line as a sterile source to transfer a cytoplasmic sterile gene into the short-foot yellow pakchoi and simultaneously transfer for more generations in one year to create the short-foot yellow pakchoi cytoplasmic gene male sterile line. The main research results are as follows 1, the green-stem pakchoi Ogrua cytoplasmic male sterile line 'P0523A' is taken as a sterile source, the yellow pakchoi excellent inbred line 'WS-28-1' is taken as a transfer target line, a directional transfer method is adopted, and a saturated backcross transfer method is adopted; 2. in order to accelerate the transformation process of the sterile line, a method of combining low-temperature vernalization treatment with greenhouse generation-adding propagation is adopted, 2 sexual generations of transformation work is completed every year, a male sterile dual-purpose line and a maintainer line similar to the horticultural traits of a target line 'WS-28-1' are created through continuous 4-generation backcross, and a dwarf yellow cabbage cytoplasmic gene male sterile line 'CMS _ 28-1A' with 100% sterile plant rate and 100% sterility is prepared.
A breeding method of yellow pakchoi Ogrua cytoplasmic male sterile line comprises the following steps:
1) carrying out hybridization by using Ogrua cytoplasmic male sterile pakchoi P0523A (2 n-20 AA, the material is from Wuhan's college of agricultural science) as a female parent and using a Chinese lobster yellow cabbage excellent inbred line ' WS-28-1 ' (2 n-20 AA, the material is from Chinese college of agricultural science) as a male parent to obtain a seed F1(AA)；
2) Full seeds F of 1) are selected in 5 months (middle ten days) of the same year1Vernalization is carried out at low temperature (2-4 ℃), and the vernalized seeds are sown in nutrient soil and grow in a greenhouse environment. The environmental temperature of the greenhouse is controlled at 24-26 ℃; when the plant is openedAfter flowering, taking the excellent inbred line 'WS-28-1' of the short-footed yellow cabbage as a recurrent male parent, and obtaining backcrossed pakchoi seeds BC in 8 months1；
3) Respectively taking the Chinese cabbages BC obtained in the step 2) in the same year in 8 months (last ten days)1And performing vernalization treatment on the seeds of the excellent inbred line 'WS-28-1' of the Brassica campestris L, sowing the seeds in the field, and performing shading cultivation. Using the Chinese cabbage seeds BC of the step 2)1Backcrossing 1 time by taking the 'WS-28-1' variety of the excellent inbred line of the yellow pakchoi as a female parent. Screening the Chinese cabbage seeds BC after the plants grow to the mature period1The individual plants with the agronomic characters similar to 'WS-28-1' in the population are planted in the greenhouse together with the excellent self-bred line 'WS-28-1' of the male parent short-foot yellow cabbage. The environmental temperature of the greenhouse is controlled at 24-26 ℃; after the buds of the plants appear in 11 months, the peripheral old leaves are removed, all the rosette leaves are removed after bolting to inhibit the growth of the side bolts, and finally, only the main bolts of the plants bloom, so that the maturing speed of the seeds is accelerated, and the Chinese cabbage seeds BC are received in the same 12 months2；
4) Sowing in 12 months (last ten days) in the same year, and sowing the Chinese cabbage seeds BC obtained in the step 3)2Repeatedly backcrossing for 1 time by taking the 'WS-28-1' variety of the excellent inbred line of the yellow pakchoi as a female parent. Screening Chinese cabbage seeds BC after the plants grow to the mature period in the early 4 months of the next year2The individual plants with the agronomic characters similar to 'WS-28-1' in the population are planted in the greenhouse together with the excellent self-bred line 'WS-28-1' of the male parent short-foot yellow cabbage. The environmental temperature of the greenhouse is controlled at 24-26 ℃; the Chinese cabbage seeds BC are received in 5 months in the next year3；
5) Sowing seeds of Chinese cabbage BC in 5 months (middle ten days) in the next year3Repeatedly backcrossing for 1 time by taking the 'WS-28-1' variety of the excellent inbred line of the yellow pakchoi as a female parent. Repeating the step 3) for 1 time, and obtaining new germplasm resources of the Ogura cytoplasmic male sterile line 'CMS _ 28-1A' with completely similar agronomic characters as the 'WS-28-1' in 8 months in the next year.
6) A method for propagating a sterile line 'CMS _ 28-1A' comprises the following steps:
A. under the condition of artificial isolation or natural isolation to avoid cross pollination;
B. taking the yellow pakchoi Ogura cytoplasmic male sterile line 'CMS _ 28-1' and the excellent yellow pakchoi inbred line 'WS-28-1' as a maintainer line;
C. according to the following steps: 1. 3: 1 or 5: 2, and harvesting seeds in the sterile line row, namely the Ogura cytoplasmic male sterile line of the Brassica napobrassica.
Through the technical measures: mainly solves the problems that the Chinese cabbage is a biennial plant, the low-temperature vernalization and long-day induction are needed for the conversion of the vegetative growth into the reproductive growth, and only one generation of sexual propagation can be completed every year under the natural condition. Aiming at the problems of more generations and slow transformation process required by the transformation of the Chinese cabbage cytoplasmic male sterile line, the invention develops the research of accelerating the transformation process of the cytoplasmic male sterile line by using the generational propagation technology. The experimental material adopts the method of combining low-temperature vernalization treatment and greenhouse generation-adding propagation by taking the excellent inbred line 'WS-28-1' of the yellow pakchoi as a transformation target strain, and only two years are needed to create the cytoplasmic gene male sterile line 'CMS-28-1A' of the yellow pakchoi with 100% sterile plant rate and 100% sterility degree, so that the field breeding is combined with the greenhouse to realize the quick transformation of the pakchoi. Compared with the prior art, the invention has the following advantages and beneficial effects:
1) in the Wuhan city of Hubei province (short days in winter and spring), Gansu province, administrative county and Chang Yanghe Shaotai town of Hubei province (long days in summer) in 6 seasons of 3 years under different sunshine and temperature conditions, the Ogura cytoplasmic male sterile line of the short-foot yellow pakchoi can show pollen-free male sterility, and the sterility rate and the sterility degree are both 100%. Therefore, the risk that fertile pollen appears in the Ogura cytoplasmic male sterile line of the Brassica campestris under the influence of temperature and illumination conditions can be avoided, and the production of Brassica campestris hybrid seeds is safer;
TABLE 1 survey of sterility under different circumstances for CMS-28-1 CMS and comparison with nuclear male sterility (recessive monogene) (2015)
2) The creation and application of the Ogura cytoplasmic male sterile line of the Brassica napobrassica improve the defect that the conventional nuclear male sterility of the Brassica napobrassica cannot be completely free of powder in China at present, and bring great risk to seed production.
3) The method for breeding the Ogura cytoplasmic male sterile line of the Brassica campestris has the advantages of simplicity, convenience, short period, high efficiency, no fertile pollen of the sterile line, no influence of temperature and illumination on sterility, stable inheritance and capability of providing reference for rapid breeding of the cytoplasmic male sterile line of the Brassica campestris and Brassicaceae plants.
Drawings
FIG. 1 is a schematic diagram of the yellow period of dwarf yellow cabbage of the breeding method of the Ogrua cytoplasmic male sterile line of the dwarf yellow cabbage.
FIG. 2 is a diagram of the flower organ morphology of PolCMS Brassica napobrassica of Brassica napobrassica Ogura cytoplasmic male sterile line.
FIG. 3 is a schematic diagram of Ogura CMS Brassica napus of Ogura cytoplasmic male sterile line of Brassica napus.
FIG. 4 is a schematic diagram of indoor generation-adding of an Ogura cytoplasmic male sterile line of Brassica campestris.
FIG. 5 is a schematic diagram of a pakchoi bagging selfing of a pakchoi Ogura cytoplasmic male sterile line.
FIG. 6 is a schematic diagram of high mountain generation-adding of pakchoi of Ogura cytoplasmic male sterile line of pakchoi.
Detailed Description
Example 1: quickly (more than one generation) breeding Ogura cytoplasmic male sterile line with yellow dwarf genetic background
1) Carrying out hybridization by using Ogrua cytoplasmic male sterile pakchoi P0523A (2n is 20 AA, the material is from the institute of agricultural science of Wuhan city) as a female parent and using a Chinese lobster yellow cabbage excellent inbred line 'WS-28-1' (2n is 20 AA, the material is from the institute of agricultural science of China) as a male parent to obtain F1 seeds (AA);
2) and (3) selecting full F1 seeds of 1) as female parents in 5 months in the same year, and taking the excellent inbred line 'WS-28-1' of the yellow pakchoi as a recurrent male parent. 30 seeds of parents and parents are taken and put into a culture dish, water is added for germination, the environmental temperature is controlled to be about 25 ℃, the seeds are put into a refrigerator at about 2 ℃ for treatment for 15 days after germination, the vernalized seeds are sown into nutrient soil and put on a tissue culture frame, and the illumination condition is set as 12 hours of illumination/12 hours of darkness. After the seedlings grow out, the cotyledons turn green, the illumination condition is changed into 24 hours of continuous illumination, and the growth is promoted until the seeds are harvested. The environmental temperature of the greenhouse is controlled to be about 25 ℃; when the plants bloom, hybridization is carried out, and backcross Chinese cabbage seeds BC1 are obtained in 8 months;
3) sowing in middle and last ten days of the same year in 8 months, backcrossing 1 time by taking the Chinese cabbage seeds BC1 of the step 2) as female parents (200 plants) and taking the variety 'WS-28-1' of the excellent inbred line of the Chinese cabbage with short foot as recurrent parents. Taking about 300 maternal seeds and about 300 paternal seeds respectively, placing the maternal seeds and the paternal seeds into a culture dish, adding water for germination, controlling the environmental temperature to be about 25 ℃, placing the maternal seeds and the paternal seeds into a refrigerator at about 2 ℃ after the seeds germinate for treatment for 15 days for low-temperature vernalization, and then sowing the vernalized seeds into the field. And (3) screening single plants with the agronomic characters similar to WS-28-1 in the Chinese cabbage seed BC1 group and the excellent inbred line WS-28-1 of the Brassica napobrassica to plant in a greenhouse after the plants grow to the period of maturity of the vegetative mass at the beginning of 10 months in the same year. The environmental temperature of the greenhouse is controlled to be about 25 ℃. After the buds of the plants appear in 12 months, removing peripheral old leaves, removing all rosette leaves after bolting, inhibiting the growth of side bolts, and finally enabling the plants to only bloom in the main bolts, so that the maturing speed of seeds is accelerated, and the Chinese cabbage seeds BC2 are received in the same 12 months;
4) sowing seeds in a greenhouse in 12 months in the same year, backcrossing for 1 time by taking the Chinese cabbage seeds BC2 of the step 3) as female parents (more than 200 plants) and taking the variety 'WS-28-1' of the excellent inbred line of the short-foot yellow cabbage as recurrent parents. And (3) screening single plants with the agronomic characters similar to WS-28-1 in the Chinese cabbage seed BC2 group and the excellent Chinese cabbage selfing line WS-28-1 to plant in a greenhouse after the plants grow to the mature period of the macronutrients in the second year 2 months. The environmental temperature of the greenhouse is controlled to be about 25 ℃. After the plants bud in the next 4 months, removing the peripheral old leaves, removing all rosette leaves after bolting, inhibiting the growth of side bolts, and finally enabling the plants to only bloom in the main bolts, so that the maturing speed of seeds is accelerated, and the Chinese cabbage seeds BC3 are received in the next 5 months;
5) and (3) sowing in the field at the beginning of 5 months in the next year, and repeatedly backcrossing for 1 time by taking the Chinese cabbage seeds BC3 in the step 4) as female parents and taking the variety 'WS-28-1' of the excellent inbred line of the Chinese cabbage with short foot as a recurrent parent. Repeating the step 3) for 1 time, and finally obtaining the Ogura cytoplasmic male sterile line 'CMS _ 28-1' with completely similar agronomic characters as 'WS-28-1' in the next year for 8 months.
Example 2: the Ogura cytoplasmic male sterile line with the yellow dwarf genetic background is bred by a conventional method (1 generation in one year)
1) Carrying out hybridization by using Ogrua cytoplasmic male sterile pakchoi P0523A (2n is 20 AA, the material is from the institute of agricultural science of Wuhan city) as a female parent and using a Chinese lobster yellow cabbage excellent inbred line 'WS-28-1' (2n is 20 AA, the material is from the institute of agricultural science of China) as a male parent, and obtaining F1 seeds (AA) in the same year and month 5;
2) and (3) selecting full F1 seeds of 1) as female parents in the same year at 10 months, and taking the excellent inbred line 'WS-28-1' of the yellow pakchoi as a recurrent male parent. Sowing the seeds in the field, and receiving Chinese cabbage seeds BC1 in the beginning of 5 months in the next year.
3) And (3) selecting full 1) BC1 seeds as female parents in 10 months in the next year, and taking the excellent inbred line 'WS-28-1' of the Brassica napobrassica as a recurrent male parent. Sowing the seeds in the field, and receiving Chinese cabbage seeds BC2 in the early 5 months of the third year.
4) And (3) selecting full 1) BC2 seeds as female parents in 10 months in the third year, and taking the excellent inbred line 'WS-28-1' of the Brassica napobrassica as a recurrent male parent. Sowing the seeds in the field, and receiving Chinese cabbage seeds BC3 at the beginning of 5 months in the fourth year.
5) And (3) selecting full 1) BC3 seeds as female parents in 10 months in the fourth year, and taking the excellent inbred line 'WS-28-1' of the Brassica napobrassica as a recurrent male parent. Sowing the seeds into the field, and finally obtaining Ogura cytoplasmic male sterile line 'CMS _ 28-1' with completely similar agronomic characters as 'WS-28-1' in the fifth year and 5 months.
Other implementation steps and conditions were the same as in example 1.
Example 3: the Ogura cytoplasmic male sterile line with the dwarf yellow genetic background is bred by adding generations in different places (2 generations in one year)
1) Carrying out hybridization by using Ogrua cytoplasmic male sterile pakchoi P0523A (2n is 20 AA, the material is from the institute of agricultural science of Wuhan city) as a female parent and using a Chinese lobster yellow cabbage excellent inbred line 'WS-28-1' (2n is 20 AA, the material is from the institute of agricultural science of China) as a male parent to obtain F1 seeds (AA);
2) selecting full F1 seeds of 1) as female parent in the same year for 5 months, taking the excellent inbred line 'WS-28-1' of the short-foot yellow cabbage as recurrent male parent, sowing the seeds to a high mountain with lower temperature (0-11 ℃) and elevation of about 1000cm, performing backcross after the plants bloom, and receiving backcross pakchoi seeds BC1 in the same year for 9 months;
3) sowing in 10-month last ten days of the same year, backcrossing 1 time by taking the Chinese cabbage seeds BC1 of the step 2) as female parents (200 plants) and taking the variety 'WS-28-1' of the excellent inbred line of the Chinese cabbage with short foot as recurrent parents. And (3) sowing the female parent seeds and the male parent seeds in open field, screening single plants with agronomic characters similar to WS-28-1 in the pakchoi seed BC1 group and the excellent inbred line WS-28-1 of the pakchoi in the same year after the plants grow to the period of maturity of the nutritive bodies within 11 months, and planting the individual plants and the excellent inbred line WS-28-1' of the pakchoi in a net house for overwintering. Backcrossing is carried out after the plants bud in the next 2 months, and Chinese cabbage seeds BC2 are received in the next 5 months;
4) selecting full 3) BC2 seeds as female parents in 5 months in the next year, taking the excellent inbred line 'WS-28-1' of the short-foot yellow cabbage as a recurrent male parent, sowing the seeds to a mountain with lower temperature (0-11 ℃) and elevation of about 1000cm, performing backcross after the plants bloom, and receiving backcross pakchoi seeds BC3 in 9 months in the next year;
5) sowing in 10-month last ten days of the next year, backcrossing 1 time by taking the Chinese cabbage seeds BC3 of the step 4) as female parents (200 plants) and taking the variety 'WS-28-1' of the excellent inbred line of the Chinese cabbage with short foot as recurrent parents. And (3) sowing the female parent seeds and the male parent seeds in the open field, and screening the single plants with the agronomic characters similar to WS-28-1 in the pakchoi seed BC1 group and the excellent inbred line WS-28-1 of the pakchoi to be planted in a net room for overwintering after the plants grow to the period of maturity of the nutritive bodies within 11 months in the next year. Backcrossing is carried out when the plants bud in 2 months in the third year, and the Ogura cytoplasmic male sterile line CMS _28-1 ' completely similar to the ' WS-28-1 ' agronomic characters is finally obtained in 5 months in the third year;
TABLE 2 transformation pathway for Ogura cytoplasmic male sterility
Claims (2)
1. A breeding method of yellow pakchoi Ogrua cytoplasmic male sterile line comprises the following steps:
1) hybridizing by using Ogrua cytoplasmic male sterile pakchoi P0523A as female parent and using Brassica rapa excellent inbred line WS-28-1 as male parent to obtain seed F1；
2) Selecting plump seeds F of step 1) in 5 months of the same year1Performing low-temperature vernalization, sowing the vernalized seeds into nutrient soil, controlling the environment temperature of a greenhouse at 24-26 ℃, and taking the excellent inbred line 'WS-28-1' of the Brassica campestris L as a recurrent male parent after the plants bloom, and obtaining backcrossed Brassica campestris seeds BC in 8 months1；
3) Respectively taking the pakchoi BC obtained in the step 2) in the same year at 8 months1Vernalizing the seeds of the excellent inbred line WS-28-1 of the Brassica napobrassica and the Brassica campestris, sowing the seeds in the field, and performing shade cultivation on the seeds with the pakchoi BC obtained in the step 2)1Backcrossing for 1 time by using the excellent self-bred line WS-28-1 variety of the Brassica campestris as female parent, growing to mature stage, and screening Brassica campestris seed BC1Planting the excellent selfing line WS-28-1 of the male parent short foot yellow Chinese cabbage into a greenhouse together, controlling the environment temperature of the greenhouse at 24-26 ℃, removing peripheral old leaves after the plants bud in 11 months, removing all lotus throne leaves after bolting, and obtaining the Chinese cabbage seeds BC in 12 months in the same year2；
4) Sowing in the same year in 12 months, and sowing the seeds BC of the Chinese cabbage in the step 3)2Repeatedly backcrossing for 1 time by taking the excellent self-bred line WS-28-1 variety of the Brassica campestris L.var.nuda as a female parent and taking the Brassica campestris L.var.nuda as a recurrent parent, growing the plantlets to the mature period in the 4 months in the next year, and screening Brassica campestris seeds BC2The individual plants with the agronomic characters and WS-28-1 in the population are simultaneously planted in the greenhouse together with the excellent inbred line WS-28-1 of the male parent Aijiaohuang cabbage, and the environmental temperature of the greenhouse is controlled at 24-At 26 deg.C, the Chinese cabbage seeds BC are received in the next 5 months3；
5) Sowing seeds of the Chinese cabbage BC in the field in the next 5 months in the next year3Taking the excellent inbred line WS-28-1 variety of the yellow pakchoi as a female parent, repeatedly backcrossing for 1 time, repeating the step 3) for 1 time, and obtaining the new germplasm resource of the Ogura cytoplasmic male sterile line CMS _28-1A with completely similar agronomic characters to WS-28-1 in 8 months in the next year.
2. The breeding method of the yellow pakchoi oguua cytoplasmic male sterile line as claimed in claim 1, wherein the breeding method comprises the following steps: under the condition of artificial isolation or natural isolation to avoid cross pollination, the sterile line CMS _28-1A uses the Brassica campestris Ogura cytoplasmic male sterile line CMS _28-1 and the Brassica campestris excellent inbred line WS-28-1 as a maintainer line according to the ratio of 2: 1. 3: 1 or 5: 2, and harvesting seeds in the sterile line row, wherein the seeds are the Ogura cytoplasmic male sterile line of the Brassica napus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910482461.XA CN112021173A (en) | 2019-06-04 | 2019-06-04 | Rapid breeding method of pakchoi Ogura cytoplasmic male sterile line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910482461.XA CN112021173A (en) | 2019-06-04 | 2019-06-04 | Rapid breeding method of pakchoi Ogura cytoplasmic male sterile line |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112021173A true CN112021173A (en) | 2020-12-04 |
Family
ID=73576127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910482461.XA Pending CN112021173A (en) | 2019-06-04 | 2019-06-04 | Rapid breeding method of pakchoi Ogura cytoplasmic male sterile line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112021173A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997009873A1 (en) * | 1995-09-11 | 1997-03-20 | Seminis Vegetable Seeds, Inc. | Cytoplasmic male sterile brassica oleracea plants which contain the polima cms cytoplasm and are male sterile at high and low temperatures |
| CN104928204A (en) * | 2015-02-03 | 2015-09-23 | 浙江省农业科学院 | Pathogenic strain causing radix curcumae bacterial wilt disease and separation method |
| CN107090495A (en) * | 2017-03-24 | 2017-08-25 | 张家口市农业科学院 | The molecular labeling related to the long character of millet neck and its detection primer and application |
| CN109593877A (en) * | 2019-01-28 | 2019-04-09 | 武汉市农业科学院 | Molecular labeling and its application with Chinese cabbage recessive gms line fertile gene close linkage |
-
2019
- 2019-06-04 CN CN201910482461.XA patent/CN112021173A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997009873A1 (en) * | 1995-09-11 | 1997-03-20 | Seminis Vegetable Seeds, Inc. | Cytoplasmic male sterile brassica oleracea plants which contain the polima cms cytoplasm and are male sterile at high and low temperatures |
| CN104928204A (en) * | 2015-02-03 | 2015-09-23 | 浙江省农业科学院 | Pathogenic strain causing radix curcumae bacterial wilt disease and separation method |
| CN107090495A (en) * | 2017-03-24 | 2017-08-25 | 张家口市农业科学院 | The molecular labeling related to the long character of millet neck and its detection primer and application |
| CN109593877A (en) * | 2019-01-28 | 2019-04-09 | 武汉市农业科学院 | Molecular labeling and its application with Chinese cabbage recessive gms line fertile gene close linkage |
Non-Patent Citations (3)
| Title |
|---|
| WU ZENGXIANG等: "Mitochondrial genome and transcriptome analysis of five alloplasmic male-sterile lines in Brassicajuncea", 《BMC GENOMICS》 * |
| 上海市种子管理总站: "上海市2015年拟通过认定非主要农作物品种目录", 《上海市农业农村委员会》 * |
| 胡侦华等: "春小白菜品种的耐抽薹性评价", 《长江蔬菜》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110100723B (en) | Hybridization breeding method of fast-period cabbage type rape and application thereof | |
| CN106962195B (en) | Cabbage seed production method | |
| CN102405826B (en) | Method for breeding inbred line by using sweet potato special materials | |
| CN100337533C (en) | Transferring and seed producing method for head cabbage cytoplasm male sterile line | |
| CN103371099A (en) | Good-quality and high-yield seed production technique of muskmelon | |
| CN105379615A (en) | Wheat blue and abortive sterile germplasm and breeding method thereof | |
| CN101263783B (en) | Method for using herbicide barban to fast breed aloe all-male plant strain | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN108703064A (en) | A kind of Small-sized watermelon cross breeding method | |
| CN110100722B (en) | Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety | |
| CN101810136A (en) | Cauliflower cytoplasmic male sterile line breeding method and application of male sterile line | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN111480569A (en) | Seed breeding method for small-fruit watermelons | |
| CN108633660B (en) | A kind of Radix Salviae Miltiorrhizae new varieties production of hybrid seeds method for culturing seedlings | |
| CN101228841B (en) | Method of using anther culture rapidly selecting wheat photo-thermoperiod sensitive genic male sterile lines | |
| CN107593302B (en) | A method of accelerating New variety of leek and cultivates process | |
| CN105075854A (en) | Breeding method for early-maturing and disease-resistant broccoli | |
| CN108849500A (en) | The culture medium and method of a kind of rescue of lotus embryo and the development of offspring's fast-growth | |
| CN108419670A (en) | A kind of long fringe neck novel rice sterile line breeding method | |
| CN105284590B (en) | The breeding method of precocity shape sponge gourd with a tight waist | |
| CN112021173A (en) | Rapid breeding method of pakchoi Ogura cytoplasmic male sterile line | |
| CN112841019A (en) | Method for breeding new species of flowering Chinese cabbage | |
| CN108651268B (en) | Breeding method of bitter gourd all-female line | |
| CN112640776A (en) | Breeding of rice photo-thermo sensitive sterile line with high outcrossing rate and seed production method thereof | |
| CN105850718A (en) | Method for prolonging rapeseed flowering period and preparation method of sterile rapeseed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201204 |