CN111972566B - Puffed sinking feed for promoting health of liver and pancreas of procambarus clarkii and preparation method thereof - Google Patents

Puffed sinking feed for promoting health of liver and pancreas of procambarus clarkii and preparation method thereof Download PDF

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CN111972566B
CN111972566B CN202010859425.3A CN202010859425A CN111972566B CN 111972566 B CN111972566 B CN 111972566B CN 202010859425 A CN202010859425 A CN 202010859425A CN 111972566 B CN111972566 B CN 111972566B
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liver
feed
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CN111972566A (en
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孔圣超
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Guan Universal Biochemical Technology Co ltd
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Guan Universal Biochemical Technology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/28Silicates, e.g. perlites, zeolites or bentonites
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/25Shaping or working-up of animal feeding-stuffs by extrusion
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The embodiment of the invention provides a puffed sinking feed for promoting the liver and pancreas health of procambarus clarkii and a preparation method thereof. The expanded sinking feed for promoting the health of the liver and the pancreas of the procambarus clarkii comprises the following components in parts by weight: 2-5 parts of fish meal, 3-8 parts of meat and bone meal, 12-15 parts of flour, 10-30 parts of soybean meal, 10-30 parts of rapeseed meal, 2-8 parts of white spirit dregs, 3-8 parts of rice bran, 3-6 parts of squid liver paste, 1-2 parts of phospholipid oil, 2-3 parts of soybean oil, 1.5-3 parts of calcium dihydrogen phosphate, 1.5-3 parts of bentonite, 1 part of shrimp premix, 0.05-0.15 part of VC ester, 0.02-0.04 part of L-carnitine, 0.02-0.04 part of taurine, 0.02-0.04 part of bile acid and 2-5 parts of sodium alginate. The expanded sinking feed provided by the embodiment of the invention can promote the growth of the procambarus clarkia and protect the liver and pancreas of the procambarus clarkia.

Description

Puffed sinking feed for promoting liver and pancreas health of procambarus clarkii and preparation method thereof
Technical Field
The invention relates to a feed for shrimps, in particular to a puffed sinking feed for promoting the health of liver and pancreas of procambarus clarkii and a preparation method thereof.
Background
Procambarus clarkii (Procambarus clarkii) named as crayfish and red crayfish belonging to the family of crayfish. It is a crustacean that grows in freshwater. With the gradual increase of the breeding density and the feed feeding amount of the procambarus clarkia, various problems begin to occur in the breeding water body environment and the shrimp body constitution, so that breeding diseases frequently occur.
The disease problem which is most concerned at present is that the liver and pancreas of the procambarus clarkii frequently have pathological changes similar to fatty liver: for example, the liver and the pancreas turn white, turn green and turn black, and even the liver tissue becomes necrotic and degenerates, which leads to the reduction and even the loss of the normal functions of the liver and the pancreas of the procambarus clarkia. The liver and pancreas, the largest and most important functional organs in the body of procambarus clarkia, has the functions of digestion, detoxification, immunity, energy storage and the like. Therefore, once the liver and pancreas are damaged, the functions of the whole organs of the procambarus clarkia are subject to collapse, which seriously affects the health and growth of the procambarus clarkia and brings great risk to the cultivation.
The current use of various antibiotics and chemical synthesis type water environment modifying agents can reduce the disease problem of Procambrus clarkii to a certain extent, but can cause a plurality of problems of drug resistance, drug residue and the like; the use of the immune vaccine is an important means for improving the disease resistance of the cultured animal body, but the practical development and development of the procambarus clarkia vaccine is not provided at present.
Disclosure of Invention
The embodiment of the invention provides a puffed sinking feed for promoting the health of the liver and the pancreas of procambarus clarkia, which can promote the growth of the procambarus clarkia and protect the liver and the pancreas of the procambarus clarkia.
A puffed sinking feed for promoting the health of liver and pancreas of procambarus clarkii comprises the following components in parts by weight: 2-5 parts of fish meal, 3-8 parts of meat and bone meal, 12-15 parts of flour, 10-30 parts of soybean meal, 10-30 parts of rapeseed meal, 2-8 parts of white spirit dregs, 3-8 parts of rice bran, 3-6 parts of squid liver paste, 1-2 parts of phospholipid oil, 2-3 parts of soybean oil, 1.5-3 parts of calcium dihydrogen phosphate, 1.5-3 parts of bentonite, 1 part of shrimp premix, 0.05-0.15 part of VC ester, 0.02-0.04 part of L-carnitine, 0.02-0.04 part of taurine, 0.02-0.04 part of bile acid and 2-5 parts of sodium alginate.
In some embodiments, the expanded settleable feed for promoting the liver and pancreas health of the procambarus clarkii comprises the following components in a weight ratio of L-carnitine, bile acid and taurine of 4.
The inventor researches and discovers that the effective combination of the L-carnitine, the bile acid and the taurine can synergistically improve the catabolism of body fat and promote the digestion, absorption, transportation and beta-oxidation of the fat, thereby ensuring the health of the liver and pancreas of the procambarus clarkia and improving the nonspecific immunity, the growth performance and the oxidation resistance of the liver and pancreas of the body. Particularly, the weight ratio of the L-carnitine, the bile acid and the taurine in the expanded settleable feed is controlled to be the proportion, so that the effect of protecting the liver and the pancreas of the procambarus clarkii can be remarkably improved, and a better synergistic effect can be exerted.
In some embodiments, in the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii, the weight ratio of the squid liver paste to the taurine is 3-6.
In some embodiments, in the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii, the weight ratio of the squid liver paste to the taurine is 4.
The research of the inventor finds that the squid liver paste and the taurine both have strong food calling performance and can improve the ingestion intensity of the procambarus clarkii, thereby improving the utilization rate of the feed and reducing the waste of the feed and the damage of the residual feed to the culture water environment. Further research shows that the weight ratio of the squid liver paste to the taurine in the puffed sinking feed is controlled to be the proportion, so that the synergistic effect can be better improved.
In some embodiments, the expanded settleable feed for promoting the health of the liver and the pancreas of the procambarus clarkia contains 2 to 5 weight percent of sodium alginate.
In some embodiments, the expanded settleable feed for promoting the health of the liver and the pancreas of the procambarus clarkia contains 3wt% of sodium alginate.
Sodium alginate is a natural polysaccharide. The inventor researches and discovers that the water resistance of the expanded sinking feed can be enhanced by adding sodium alginate into the expanded sinking feed, and the water resistance time of the expanded sinking feed can be generally prolonged to be more than 4 hours. In addition, the proportion of the starch raw materials in the formula can be reduced by adding a certain proportion of sodium alginate into the expanded sinking feed, so that the load of excessive starch on the liver and pancreas of the procambarus clarkii is reduced, the incidence of fatty liver is reduced, and the formula cost is optimized. The content of starch raw materials in the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii in the embodiment of the invention is generally 12-15wt%. If sodium alginate is not added, the content of the starch raw materials needs to be increased to 17-20wt% to achieve the original water-resistant effect of the procambarus clarkia feed in the embodiment of the invention.
In some embodiments, the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii comprises the following components in parts by weight: 2 to 3 parts of fish meal, 5 to 5.1 parts of meat and bone meal, 13 to 14 parts of flour, 29.5 to 30 parts of bean pulp, 22.5 to 25 parts of rapeseed meal, 5 to 5.5 parts of white spirit residue, 3.8 to 4 parts of rice bran, 4 parts of squid extract, 1.5 to 2 parts of phosphatide oil, 2 to 2.5 parts of soybean oil, 2 to 2.2 parts of monocalcium phosphate, 2 parts of bentonite, 1 part of shrimp premix, 0.1 to 0.11 part of VC ester, 0.04 part of L-carnitine, 0.03 part of taurine, 0.03 part of bile acid and 3 parts of sodium alginate.
Herein, the VC ester refers to vitamin C phosphate.
In some embodiments, the vitamin C content of the VC ester is equal to or greater than 35wt%. Commercially available.
In the embodiment of the invention, the high-content VC phosphate is selected as the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkia, so that the anti-stress capability and the autoimmunity of the procambarus clarkia to the external environment can be improved, and the metabolism of fat and lipoid, particularly cholesterol, can be improved.
In some embodiments, the expanded heavy feed for promoting the liver and pancreas health of the procambarus clarkii has a crude protein content of more than or equal to 30wt% and a crude fat content of more than or equal to 6.5wt%.
In some embodiments, the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii has a crude protein content of 30.4wt% and a crude fat content of 6.9wt%.
In the embodiment of the invention, the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkia has sufficient crude fat content, rich fat is beneficial to absorption and transportation of fat-soluble vitamins in vivo, essential fatty acid can be provided for the procambarus clarkia, protein can be saved, and the utilization rate of the feed protein can be improved.
In some embodiments, the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii is composed of or prepared from the components.
In some embodiments, the total composition of the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii is 100 parts.
In some embodiments, the expanded settleable feed for promoting the liver and pancreas health of the procambarus clarkii contains 0.04wt%, 0.03wt% and 0.03wt% of L-carnitine, bile acid and taurine respectively.
In some embodiments, the premix for shrimp is a conventional raw material in the art and is commercially available. For example, the model 2116 of crayfish compound premix provided by Beijing Sangpu Biochemical technology Limited can be selected.
In some embodiments, the premix for shrimp comprises per kilogram: 45 ten thousand IU/kg of vitamin A, 15-30 ten thousand IU/kg of vitamin D, more than or equal to 3300mg/kg of vitamin E, more than or equal to 550mg/kg of vitamin K3, more than or equal to 850mg/kg of vitamin B1, more than or equal to 850mg/kg of vitamin B2, more than or equal to 1100mg/kg of vitamin B6, more than or equal to 2mg/kg of vitamin B12, more than or equal to 2800mg/kg of nicotinamide, more than or equal to 2800mg/kg of D-calcium pantothenate, more than or equal to 500mg/kg of folic acid, more than or equal to 2mg/kg of biotin, more than or equal to 7000mg/kg of inositol, more than or equal to 24000mg/kg of 35 VC phosphate, 12-75 g/kg of iron, 1.8-5 g/kg of copper, 1-3 g/kg of manganese, 3-6 g/kg of zinc, more than or equal to 20g/kg of magnesium, 200-1000 mg/kg of iodine, 40-50 mg/kg of selenium and 20-50 mg/kg of cobalt.
In some embodiments, the expanded sinking feed for promoting the liver and pancreas health of procambarus clarkii is a pellet feed.
In some embodiments, the expanded settleable feed for promoting the health of the liver and the pancreas of the procambarus clarkii has a particle size of 1.0-3.0mm so as to meet the requirement of the procambarus clarkii on the particle size of the feed in different growth periods, thereby being more beneficial to the ingestion of the feed by the procambarus clarkii.
The invention also provides a preparation method of the expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii, which comprises the steps of mixing the components according to the proportion, carrying out superfine crushing, modifying, extruding, expanding, granulating and drying.
The embodiment of the invention also provides application of the expanded sinking feed for promoting the health of the liver and the pancreas of the procambarus clarkia in preparation of the procambarus clarkia feed. The application specifically comprises the functions of promoting the health of the liver and the pancreas of the procambarus clarkii, and improving the growth performance, the non-specific immunity and the oxidation resistance of the liver and the pancreas of the procambarus clarkii.
The expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii provided by the embodiment of the invention can meet the nutritional requirements of the procambarus clarkii in different growth stages.
The research shows that the expanded sinking feed for promoting the health of the liver and the pancreas of the procambarus clarkia provided by the embodiment of the invention contains rich high-quality fat, and simultaneously the oxidation resistance of the liver and the pancreas of the procambarus clarkia is enhanced, so that the astaxanthin with fat solubility in the body is beneficial to absorption, storage and transportation in the procambarus clarkia body, thereby better improving the body color of the procambarus clarkia.
Compared with the prior art, the invention has the following advantages:
1) According to the embodiment of the invention, the squid liver paste and the taurine in the expanded settleable feed both have strong food calling performance, and the ingestion strength of procambarus clarkii can be improved, so that the utilization rate of the feed is improved, and the waste of the feed and the damage of residual feed of the feed to the culture water environment are reduced.
2) The sodium alginate in the expanded sinking feed of the embodiment of the invention is a natural polysaccharide, has strong viscosity, stability, solubility and safety, can enhance the water resistance of the feed in water, and generally can prolong the water resistance time of the procambarus clarkia feed to more than 4 hours. The proportion of starch raw materials in the formula can be reduced by adding a certain proportion of sodium alginate into the feed, so that the load of excessive starch on the liver and pancreas of the procambarus clarkii is reduced, the incidence rate of fatty liver is reduced, and the formula cost is optimized.
3) The high-content VC phosphate ester in the expanded settleable feed provided by the embodiment of the invention can improve the anti-stress capability and the autoimmunity of the procambarus clarkia to the external environment, and can improve the metabolism of fat and lipoid, especially cholesterol.
4) The effective combination of the L-carnitine, the bile acid and the taurine in the expanded sinking feed can synergistically improve the catabolism of body fat and promote the digestion, absorption, transportation and beta-oxidation of the fat, thereby ensuring the health of the liver and the pancreas of the procambarus clarkii, and improving the nonspecific immunity, the growth performance and the oxidation resistance of the liver and the pancreas of the body; when the mass percentages of L-carnitine, bile acid and taurine added in the feed are respectively 0.04wt%, 0.03wt% and 0.03wt% (namely the ratio is 4.
5) The expanded sinking feed has sufficient crude fat content, rich fat is beneficial to absorption of fat-soluble vitamins and transportation in vivo, and meanwhile, the expanded sinking feed can provide essential fatty acid for the procambarus clarkia, can save protein and improve the utilization rate of feed protein.
Detailed Description
The present invention will be further described with reference to the following examples.
The premix used below is a crawfish compound premix 2116 of Beijing Sangpu Biochemical technology Limited.
The following VC esters (35% type) were provided as 35% type VC esters by Beijing Sangpu Biochemical technology, inc.
Example 1
The expanded sinking feed for promoting the health of the liver and the pancreas of the procambarus clarkii comprises the following components: 2kg of fish meal, 5kg of meat and bone meal, 13kg of flour, 30kg of soybean meal, 25kg of rapeseed meal, 5kg of white spirit residue, 3.8kg of rice bran, 4kg of squid extract, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 2kg of bentonite, 1kg of shrimp premix, 0.11kg of VC ester (35 percent type), 0.04kg of L-carnitine, 0.03kg of taurine, 0.03kg of bile acid and 3kg of sodium alginate.
The preparation method of the expanded sinking feed comprises the following steps: mixing the raw material components of the feed, performing superfine crushing, conditioning, extruding and puffing, granulating and drying to prepare the puffed granular material with the size of 1.0-3.0 mm.
Example 2
The embodiment of the expanded settleable feed for promoting the liver and pancreas health of the procambarus clarkii comprises 2.2kg of fish meal, 7kg of meat and bone meal, 13.5kg of flour, 28.5kg of soybean meal, 23.5kg of rapeseed meal, 5kg of white spirit residue, 4.8kg of rice bran, 3.8kg of squid extract, 2kg of phospholipid oil, 2kg of soybean oil, 2.2kg of monocalcium phosphate, 1.8kg of bentonite, 1kg of a shrimp premix, 0.1kg of VC ester (35 percent type), 0.04kg of L-carnitine, 0.03kg of taurine, 0.03kg of bile acid and 2.5kg of sodium alginate.
The preparation method of the expanded sinking feed is basically the same as that of the expanded sinking feed in the embodiment 1.
Example 3
The expanded sinking feed for promoting the liver and pancreas health of the procambarus clarkii comprises the following components: 3kg of fish meal, 5.1kg of meat and bone meal, 14kg of flour, 29.5kg of soybean meal, 22.5kg of rapeseed meal, 5.5kg of white spirit residues, 4.0kg of rice bran, 4.0kg of squid paste, 1.5kg of phospholipid oil, 2.5kg of soybean oil, 2.2kg of monocalcium phosphate, 2kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type), 0.04kg of L-carnitine, 0.03kg of taurine, 0.03kg of bile acid and 3kg of sodium alginate.
The preparation method of the expanded sinking feed is basically the same as that of the expanded sinking feed in the embodiment 1.
Example 4
The embodiment of the expanded settleable feed for promoting the health of the liver and the pancreas of the procambarus clarkia comprises 2.8kg of fish meal, 5kg of meat and bone meal, 12.5kg of flour, 28kg of bean pulp, 26kg of rapeseed meal, 5kg of white spirit residue, 4kg of rice bran, 4.0kg of squid extract, 1.5kg of phospholipid oil, 2kg of soybean oil, 2.5kg of monocalcium phosphate, 2kg of bentonite, 1kg of a premix for the procambarus clarkia, 0.1kg of VC ester (35 percent type), 0.04kg of L-carnitine, 0.03kg of taurine, 0.03kg of bile acid and 3.5kg of sodium alginate.
The preparation method of the expanded sinking feed is basically the same as that of the expanded sinking feed in the embodiment 1.
Example 5
The embodiment of the expanded settleable feed for promoting the health of the liver and the pancreas of the procambarus clarkii comprises the following components
The following raw materials are respectively weighed: 2.5kg of fish meal, 4kg of meat and bone meal, 14kg of flour, 26.5kg of soybean meal, 29.3kg of rapeseed meal, 3.95kg of white spirit residues, 4kg of rice bran, 4.5kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2.5kg of monocalcium phosphate, 1.5kg of bentonite, 1kg of shrimp premix, 0.15kg of VC ester (35 percent type), 0.04kg of L-carnitine, 0.03kg of taurine, 0.03kg of bile acid and 2kg of sodium alginate.
The preparation method of the expanded sinking feed is basically the same as that of the expanded sinking feed in the embodiment 1.
Example 6
The embodiment of the expanded settleable feed for promoting the liver and pancreas health of procambarus clarkii comprises 2.8kg of fish meal, 4kg of meat and bone meal, 13.5kg of flour, 28kg of soybean meal, 27kg of rapeseed meal, 5kg of white spirit residue, 4kg of rice bran, 4kg of squid extract, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 2kg of bentonite, 1kg of a shrimp premix, 0.1kg of VC ester (35% type), 0.04kg of L-carnitine, 0.03kg of taurine, 0.03kg of bile acid and 2.5kg of sodium alginate.
The preparation method of the expanded sinking feed is basically the same as that of the embodiment 1.
Comparative example 1
The expanded settleable feed for procambarus clarkia in the comparative example consists of the following components: 2.5kg of fish meal, 3.5kg of meat and bone meal, 12kg of flour, 27kg of soybean meal, 28kg of rapeseed meal, 6kg of white spirit residue, 4.9kg of rice bran, 4kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 2kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type) and 3kg of sodium alginate.
The preparation method of the comparative example expanded sinking feed comprises the following steps: mixing the raw material components of the feed, performing superfine crushing, conditioning, extruding and puffing, granulating and drying to prepare the puffed granular material with the size of 1.0-3.0 mm.
Comparative example 2
The expanded settleable feed for procambarus clarkia in the comparative example consists of the following components: 2.5kg of fish meal, 3.5kg of meat and bone meal, 12kg of flour, 27kg of soybean meal, 28kg of rapeseed meal, 6kg of white spirit residue, 4.9kg of rice bran, 4kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 1.96kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type), 0.04kg of taurine and 3kg of sodium alginate.
The preparation method of the comparative example expanded settleable feed comprises the following steps: mixing the raw materials, micronizing, modifying, extruding, puffing, granulating, and oven drying to obtain 1.0-3.0mm puffed granule
Comparative example 3
The expanded settleable feed for procambarus clarkia in the comparative example consists of the following components: 2.5kg of fish meal, 3.5kg of meat and bone meal, 12kg of flour, 27kg of soybean meal, 28kg of rapeseed meal, 6kg of white spirit residue, 4.9kg of rice bran, 4kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 1.98kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type), 0.02kg of taurine and 3kg of sodium alginate.
The preparation method of the comparative example expanded settleable feed comprises the following steps: mixing the raw materials, micronizing, modifying, extruding, puffing, granulating, and oven drying to obtain 1.0-3.0mm puffed granule
Comparative example 4
The expanded settleable feed for procambarus clarkia in the comparative example consists of the following components: 2.5kg of fish meal, 3.5kg of meat and bone meal, 12kg of flour, 27kg of soybean meal, 28kg of rapeseed meal, 6kg of white spirit residue, 4.9kg of rice bran, 4kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 1.97kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type), 0.03kg of taurine and 3kg of sodium alginate.
The preparation method of the comparative example puffed sinking feed comprises the following steps: mixing the raw materials, micronizing, modifying, extruding, puffing, granulating, and oven drying to obtain 1.0-3.0mm puffed granule
Comparative example 5
The expanded settleable feed for procambarus clarkia in the comparative example consists of the following components: 2.5kg of fish meal, 3.5kg of meat and bone meal, 12kg of flour, 27kg of soybean meal, 28kg of rapeseed meal, 6kg of white spirit residue, 4.9kg of rice bran, 4kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 1.9kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type), 0.03kg of L-carnitine, 0.03kg of taurine, 0.04kg of bile acid and 3kg of sodium alginate.
The preparation method of the comparative example puffed sinking feed comprises the following steps: mixing the raw materials, micronizing, modifying, extruding, puffing, granulating, and oven drying to obtain 1.0-3.0mm puffed granule
Comparative example 6
The expanded settleable feed for procambarus clarkia in the comparative example comprises the following components: 2.5kg of fish meal, 3.5kg of meat and bone meal, 12kg of flour, 27kg of soybean meal, 28kg of rapeseed meal, 6kg of white spirit residue, 4.9kg of rice bran, 4kg of squid paste, 2kg of phospholipid oil, 2kg of soybean oil, 2kg of monocalcium phosphate, 1.9kg of bentonite, 1kg of shrimp premix, 0.1kg of VC ester (35 percent type), 0.05kg of L-carnitine, 0.03kg of taurine, 0.02kg of bile acid and 3kg of sodium alginate.
The preparation method of the comparative example puffed sinking feed comprises the following steps: mixing the raw materials, micronizing, modifying, extruding, puffing, granulating, and oven drying to obtain 1.0-3.0mm puffed granule
Examples of the experiments
A comparative breeding test was conducted using the expanded settleable feeds for procambarus clarkia prepared in examples 1 and 3 and comparative examples 1 to 6.
After the same batch of procambarus clarkii fries are selected for temporary culture and domestication for 7 days, the procambarus clarkii fries with strong physique and basically consistent size are selected as experimental objects, and the average weight is (4.5 +/-0.5) g.
The experiment is provided with 8 groups, each group is provided with 3 parallel net cages (the specification is 3m multiplied by 2 m), the total number of the net cages is 24, 10 procambarus clarkii are put in each net cage, and the net cages are evenly placed in the total area of 6670m 2 In a pond. 8 groups of feeds of the expanded sinking feeds of the procambarus clarkii in the examples 1 and 3 and the comparative examples 1 to 6 are respectively fed. After the breeding experiment begins, each group of experimental feed is fed to the shrimp seedlings in the net cage, the number of daily fed meals is one meal, the time point is set at 17 percent, a feeder is used for uniformly feeding, the daily feeding rate is 2 to 5 percent, and the specific feeding rate can be correspondingly adjusted along with the conditions of water quality, weather, shrimp body health and the like. In the experimental period, the water quality and the substrate are regulated once every 5 to 7 days, the water transparency of about 40cm is ensured, and the concentrations of harmful substances such as sub-salt, ammonia nitrogen, hydrogen sulfide and the like in the water are well controlled; the bottom of the pond is provided with the microporous tube oxygen increasing equipment, so that the dissolved oxygen content of the water body is ensured to be sufficient, and the normal feeding and growth of the procambarus clarkii in the experimental net cage are facilitated.
And (4) continuously culturing for 4 weeks in the experiment, stopping feeding 24 hours before detection, respectively counting the number and the weight of the procambarus clarkia in each net cage, recording the total feed feeding amount of each net cage, and calculating the survival rate, the weight gain rate and the bait coefficient. After the experiment is finished, the blood biochemical indexes are measured: randomly selecting 6 procambarus clarkii from each net cage, collecting blood in the heart surrounding cavity of the cephalothorax by using a disposable medical syringe, storing the collected blood in a sterile centrifuge tube, standing for 1-2h in a refrigerator at 4 ℃, and centrifuging for 10min at 3000r/min at 4 ℃ to prepare serum for measuring Total Protein (TP), lysozyme (LSZ), glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT). The determination of the plasma TP concentration adopts a Coomassie brilliant blue staining method and then uses Nanjing to establish a kit of a bioengineering institute to detect other indexes; and then, measuring an antioxidant index: cutting open the head and chest beetle immediately after the blood collection of the shrimp body, stripping off the liver and pancreas, washing the liver and pancreas tissues with normal saline, then sucking off the surface water with filter paper, putting a proper amount of liver and pancreas samples into a freezing tube, placing the samples into liquid nitrogen for quick freezing, and then storing the samples in a refrigerator at the temperature of 20 ℃ below zero. Thawing a liver and pancreas sample, washing, weighing, homogenizing the liver and pancreas and homogenate liquid in an ice bath according to the proportion of 1.
The experimental data are expressed as the mean value plus or minus standard deviation, and SPSS22.0 statistical software is adopted to carry out single-factor variance analysis and Duncan's multiple comparison on the experimental data so as to test the difference among the groups; when P <0.05, the statistical results are statistically significant. The results are shown in tables 1, 2 and 3.
TABLE 1 Effect of different experimental feeds on the growth Performance of Procambrus clarkii
Figure BDA0002647559620000091
Note: different lower case letters indicate that the data differences between groups are statistically significant (P < 0.05).
The quality of the terminal body, the weight gain rate, the survival rate and the bait coefficient are important indexes for evaluating the growth performance of the animals, and the growth condition of the cultured animals can be intuitively reflected. As can be seen from table 1, the growth performance of the groups of example 1 and 3 of the present invention was significantly improved compared to the groups of the comparative examples 1 to 6 of the procambarus clarkii. In terms of terminal body mass, groups 1 and 3 of examples 1 and 3 of the present invention were significantly larger than groups 1 to 6 of comparative examples, wherein groups 5 to 6 of comparative examples were significantly larger than groups 1 to 4 of comparative examples, and group 4 of comparative examples was significantly larger than groups 1 to 3 of comparative examples; the inventive examples 1 and 3 groups were significantly higher in weight gain than the comparative examples 1-6 groups, with comparative examples 5-6 groups being significantly higher than the comparative examples 1-4 groups; in terms of survival rate, the survival rate is not significant among groups; the inventive example 1 and 3 groups were significantly smaller than the comparative examples 1-6 groups in terms of bait coefficient, with comparative examples 5-6 groups being significantly smaller than comparative examples 1-4 groups and comparative example 4 group being significantly smaller than comparative examples 1-3 groups.
TABLE 2 influence of different experimental feeds on the biochemical indexes of the blood serum of Procambrus clarkii
Figure BDA0002647559620000101
Note: different lower case letters indicate that the data differences between groups are statistically significant (P < 0.05).
Lysozyme is an important nonspecific immune factor, the immunity of organisms is enhanced along with the rise of the lysozyme activity, and the total protein concentration is correspondingly improved. Normally, serum glutamate pyruvate transaminase and glutamate oxaloacetate transaminase are at low levels of activity, but when liver cells are damaged and their cell membrane permeability becomes high, the amino acid transaminases penetrate a large amount into the blood. As can be seen from Table 2, the levels of lysozyme in the groups of examples 1 and 3 of the present invention were significantly higher than those in the groups of comparative examples 1 to 6; the total protein concentration of the groups 1 and 3 of the invention is only obviously higher than that of the groups 1 to 3 of the comparative examples; the levels of glutamate pyruvate transaminase were significantly lower in the groups of examples 1 and 3 of the present invention than in the groups of comparative examples 1-6, where the groups of comparative examples 5-6 were significantly lower than in the groups of comparative examples 1-4; the levels of glutamic-oxaloacetic transaminase in the inventive groups 1 and 3 were significantly lower than in the comparative groups 1-6.
TABLE 3 influence of different experimental feeds on the antioxidant capacity of the liver and pancreas of procambarus clarkii
Figure BDA0002647559620000102
Figure BDA0002647559620000111
Note: different lower case letters indicate that the difference in data between groups was statistically significant (P < 0.05).
The antioxidase system established by superoxide dismutase, catalase, glutathione and the like can decompose excessive free radicals, so that the peroxidation of body lipid is slowed down, and the tissue cells are prevented from being damaged by more peroxidation products, so that the activities of the 3 biochemical indexes can intuitively reflect the antioxidant capacity of the animal body. As can be seen from Table 3, the activity of the superoxide dismutase of the groups 1 and 3 of the invention is significantly higher than that of the groups 1 to 6 of the comparative examples; the catalase activity of the groups 1 and 3 of the examples of the invention is significantly higher than that of the groups 1 to 6 of the comparative examples, wherein the groups 5 to 6 of the comparative examples are significantly higher than that of the groups 1 to 4 of the comparative examples, the group 4 of the comparative examples is significantly higher than that of the groups 1 to 3 of the comparative examples, and the groups 2 to 3 of the comparative examples are significantly higher than that of the group 1 of the comparative examples; the glutathione activities of the groups 1 and 3 of the example are significantly higher than those of the groups 1 to 6 of the comparative example, wherein the groups 5 to 6 of the comparative example are significantly higher than those of the groups 1 to 4 of the comparative example.
In conclusion, the expanded sinking feed can promote the health of the liver and the pancreas of the procambarus clarkii, and can improve the growth performance, the non-specific immunity and the oxidation resistance of the liver and the pancreas of the procambarus clarkii.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (9)

1. A puffed sinking feed for promoting the health of liver and pancreas of procambarus clarkii comprises the following components in parts by weight: 2-5 parts of fish meal, 3-8 parts of meat and bone meal, 12-15 parts of flour, 10-30 parts of soybean meal, 10-30 parts of rapeseed meal, 2-8 parts of white spirit dregs, 3-8 parts of rice bran, 3-6 parts of squid liver paste, 1-2 parts of phospholipid oil, 2-3 parts of soybean oil, 1.5-3 parts of calcium dihydrogen phosphate, 1.5-3 parts of bentonite, 1 part of shrimp premix, 0.05-0.15 part of VC ester, 0.02-0.04 part of L-carnitine, 0.02-0.04 part of taurine, 0.02-0.04 part of bile acid and 2-5 parts of sodium alginate;
wherein the weight ratio of L-carnitine, bile acid and taurine in the expanded sedimentary feed is 4.
2. The expanded sinking feed for promoting the liver and pancreas health of procambarus clarkii according to claim 1, wherein the weight ratio of squid liver paste to taurine in the expanded sinking feed is (3-6) to (0.02-0.04).
3. The expanded sinking feed for promoting the liver and pancreas health of procambarus clarkii according to claim 1, wherein the weight ratio of squid liver paste to taurine in the expanded sinking feed is 4.
4. The expanded sinking feed for promoting the liver and pancreas health of procambarus clarkii according to any one of claims 1 to 3, wherein the content of sodium alginate in the expanded sinking feed is 2 to 5wt%.
5. The expanded sinking feed for promoting the liver and pancreas health of procambarus clarkii according to claim 4, wherein the content of sodium alginate in the expanded sinking feed is 3wt%.
6. The expanded settleable feed for promoting the health of the liver and the pancreas of procambarus clarkii according to claim 1, which comprises the following components in parts by weight: 2-3 parts of fish meal, 5-5.1 parts of meat and bone meal, 13-14 parts of flour, 29.5-30 parts of soybean meal, 22.5-25 parts of rapeseed meal, 5-5.5 parts of white spirit residue, 3.8-4 parts of rice bran, 4 parts of squid paste, 1.5-2 parts of phospholipid oil, 2-2.5 parts of soybean oil, 2-2.2 parts of monocalcium phosphate, 2 parts of bentonite, 1 part of shrimp premix, 0.1-0.11 part of VC ester, 0.04 part of L-carnitine, 0.03 part of taurine, 0.03 part of bile acid and 3 parts of sodium alginate.
7. The expanded settleable feed for promoting the health of the liver and the pancreas of procambarus clarkii according to any one of claims 1 to 3, 5 or 6, wherein the content of vitamin C in the VC ester is equal to or more than 35wt%.
8. The method for preparing an expanded sinking feed for promoting the liver and pancreas health of procambarus clarkii according to any one of claims 1 to 7, which comprises the steps of mixing the components according to a certain proportion, carrying out superfine crushing, modifying, extruding, expanding, granulating and drying.
9. The use of the expanded settleable feed of any one of claims 1 to 7 for promoting the health of the liver and pancreas of procambarus clarkia in the preparation of feed for procambarus clarkia.
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