CN111955485A - Method for improving application effect of trichoderma in orchard - Google Patents
Method for improving application effect of trichoderma in orchard Download PDFInfo
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- CN111955485A CN111955485A CN202010972331.7A CN202010972331A CN111955485A CN 111955485 A CN111955485 A CN 111955485A CN 202010972331 A CN202010972331 A CN 202010972331A CN 111955485 A CN111955485 A CN 111955485A
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- trichoderma
- chitosan
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- polyglutamic acid
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
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Abstract
The invention provides a method for improving the application effect of trichoderma in an orchard, and belongs to the technical field of fruit tree maintenance. The invention comprises the following steps: (1) fully and uniformly mixing the trichoderma preparation and clear water, and adding gamma-polyglutamic acid and sugarcane waste molasses or molasses alcohol fermentation liquor to obtain suspension; (2) uniformly spraying the suspension prepared in the step (1) on the branches of the fruit trees; (3) and (3) immediately spraying chitosan borate solution when the suspension is not dried, so that the chitosan borate and the gamma-polyglutamic acid are polymerized, and an antibacterial film is formed on the outer layer. The method can improve the adhering and colonization capacity of the microbial inoculum on the surface of the tree branches and the trunk, improve the spore production and antagonistic capacity of the microbial inoculum, further improve the proliferation capacity of the microbial inoculum in an orchard and improve the effect of improving the microbial ecological condition; the decomposition capability of the microbial inoculum on the old skins of the grapevine body is improved, the old skins are promoted to fall off, and the hiding space of plant diseases and insect pests is reduced.
Description
Technical Field
The invention belongs to the technical field of fruit tree maintenance, and particularly relates to a method for improving the application effect of trichoderma in an orchard.
Background
With the development of society, people are increasingly aware that the use of chemical pesticides comes at the cost of sacrificing ecological environment and human health, and biological control aiming at agricultural sustainable development plays an increasingly important role in agricultural production. The biological control is a method for controlling harmful organisms by using beneficial organisms or metabolites thereof, and has the advantages of safety to human and livestock, no residual toxicity, no pollution to environment and fruits, no resistance to the harmful organisms, no damage to ecological balance, economy and effectiveness.
Trichoderma is reported to be one of the most commonly used biocontrol agents at present. Many attempts and intensive research into the use of biocontrol of trichoderma, the development of biopharmaceuticals, and biocontrol mechanisms have been conducted for many years. The antagonistic action of trichoderma on phytopathogenic fungi involves the following mechanisms: firstly, the trichoderma has a competitive effect, and the trichoderma weakens or even eliminates pathogenic bacteria in the same environment through rapid propagation and growth for competing for nutrients, occupying living space, consuming nutrients and the like, so that the aim of antagonizing the pathogenic bacteria is fulfilled. And secondly, antibacterial substances are generated, a plurality of trichoderma strains generate volatile or nonvolatile antibiotic substances, the antibiotic substances generated in the metabolic process of trichoderma comprise trichoderma, gliocladycin, chloromycetin, antibacterial peptide and the like, and the secondary metabolites show positive control effect on certain pathogenic fungi. And thirdly, the trichoderma hyphae grow in parallel and spirally winding along the host hyphae, generate attached cellular branches which are adsorbed on the host hyphae, further dissolve cell walls by secreting extracellular enzymes such as chitinase, glucanase, cellulase, protease and the like, penetrate the host hyphae, absorb nutrition and disintegrate pathogenic fungi hypha cells. Meanwhile, the different cell wall degrading enzymes of trichoderma have synergistic effects and different antagonistic modes. It can be said that the antagonistic bacteria of trichoderma with various biological control weapons is a more ideal biological control beneficial bacteria.
At present, most of the existing methods for applying the biocontrol trichoderma fungicide in orchards such as vineyards are simple full-garden spraying, and are influenced by adverse factors such as oligotrophism of the surface epiphytic environment of plants, unstable temperature and humidity conditions, high ultraviolet intensity and the like, so that the biocontrol fungicide is difficult to obtain the environmental conditions capable of stably colonizing and proliferating, and the problems of short drug effect period, large use amount, high use frequency, poor effect of improving the microbial ecological condition of the orchards and the like are caused when the biocontrol fungicide is used.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a novel application method of a biocontrol trichoderma fungicide, which can improve the attachment and colonization capacity of the fungicide on the epidermis of the trunk and branch of a tree and improve the spore production and antagonism capacity of the fungicide, thereby improving the proliferation capacity of the fungicide in an orchard and improving the effect of improving the ecological condition of microorganisms; the method can prolong the use drug effect of the biological control agent, and reduce the use amount and use frequency of the biological control agent; the invention can improve the decomposition capability of the microbial inoculum on the old bark of the fruit tree body, further promote the old bark to fall off and reduce the hiding space of plant diseases and insect pests.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method of increasing trichoderma application in orchards comprising the steps of:
(1) fully and uniformly mixing the trichoderma preparation with clear water according to the use concentration requirement of the trichoderma preparation, adding gamma-polyglutamic acid and sugarcane waste molasses or adding gamma-polyglutamic acid and molasses alcohol fermentation liquor, and uniformly mixing to obtain suspension;
(2) uniformly spraying the suspension prepared in the step (1) on the branches of the fruit trees;
(3) and (3) immediately spraying chitosan borate solution when the suspension is not dried, so that the chitosan borate and the gamma-polyglutamic acid are polymerized, and an antibacterial film is formed on the outer layer.
Further, the trichoderma is any one of trichoderma viride, trichoderma harzianum and trichoderma longibrachiatum.
Further, the orchard is a vineyard, and the fruit tree branches are grape branches.
Further, in the step (1), the final concentration of the added gamma-polyglutamic acid is 300-1000 mg/L; the final Baume value of the added sugarcane waste molasses or molasses alcohol fermentation liquor is 0.5-5 DEG Be.
Further, in the step (3), the concentration of chitosan in the chitosan boric acid solution is 1-10 g/L.
Further, the preparation method of the chitosan boric acid solution comprises the following steps: dissolving 1g of chitosan with deacetylation degree of more than 75% in 100mL of boric acid aqueous solution with concentration of 5-10g/L, and diluting twice according to actual requirements to obtain the chitosan boric acid solution.
Has the advantages that:
the method comprises the steps of mixing a trichoderma agent with gamma-polyglutamic acid, molasses alcohol fermentation liquor or cane molasses to obtain a suspension, spraying a chitosan borate solution on the branches and trunks of fruit trees, polymerizing chitosan borate with the gamma-polyglutamic acid, forming a selective bacteriostasis membrane on the outer layer, forming a microenvironment with relatively stable humidity and relatively sufficient nutrition and absolute advantage of trichoderma agent biomass on the inner side of the bacteriostasis membrane, continuously generating a large number of conidia, antagonistic substances and cellulase under the induction of the cane molasses or the molasses alcohol fermentation liquor, enabling the conidia to float and germinate again in an orchard environment, forming a microbial ecological advantage, and inhibiting the growth of harmful microorganisms; the antagonistic substance inhibits harmful microorganisms attached to the surface of the grape plant, induces the immune response of the plant and improves the disease resistance; the cellulase decomposes the old skin tissue, promotes the old skin tissue to decompose and fall off, and effectively reduces the hiding space of plant diseases and insect pests.
The compound of the gamma-polyglutamic acid and the sugarcane waste molasses or the molasses alcohol fermentation liquor can absorb water in the air, promote a microbial inoculum suspension membrane to keep humidity and improve the environment for the growth and proliferation of the biocontrol microbial inoculum; meanwhile, the chitosan borate and the gamma-polyglutamic acid are polymerized to form a selective antibacterial film on the outer layer, so that the growth advantage of harmful microorganisms which cannot utilize the chitosan is prevented. The selective antibacterial membrane structure formed by the method can improve the adhesion effect of the microbial inoculum suspension.
The method can improve the efficacy of the biocontrol microbial inoculum and prolong the action time of the biocontrol microbial inoculum.
Drawings
FIG. 1 is a comparison graph of a test for producing bacteriostatic substances by inducing trichoderma with molasses alcohol fermentation liquor; in the figure, A is a pathogenic bacterium (Botrytis cinerea) which normally grows, C is an inhibitory effect of Trichoderma against the pathogenic bacterium when molasses alcohol fermentation broth is not added, and B and D are inhibitory effects of Trichoderma against the pathogenic bacterium when molasses alcohol fermentation broth is added.
Detailed Description
The invention is further described with reference to the following drawings and specific embodiments.
Example 1
A method for improving trichoderma application effect of an orchard, particularly a vineyard, comprises the following steps:
(1) fully and uniformly mixing a 'Yingcaimeidi' brand trichoderma viride agent produced by Shandong Changtai biological science and technology limited company in the market with clear water according to the concentration and the dosage of a commodity instruction, adding gamma-polyglutamic acid (the final concentration is 300mg/L) and molasses alcohol fermentation liquor (the final Baume value is 2 DEG Be), and uniformly mixing to obtain suspension;
(2) uniformly spraying the suspension prepared in the step (1) on grape branches, particularly on the part covered by the old skin;
(3) immediately spraying chitosan borate solution (the chitosan borate solution is prepared by dissolving 1g of chitosan with deacetylation degree of 85% in 100mL of boric acid aqueous solution with concentration of 5 g/L) when the suspension is not dried; the chitosan borate and the gamma-polyglutamic acid are polymerized to form a selective antibacterial film on the outer layer, so that the growth advantage of harmful microorganisms which can not utilize the chitosan is prevented. A microenvironment with relatively stable humidity, relatively sufficient nutrition and absolute advantage of trichoderma agent biomass is formed on the inner side of the bacteriostatic membrane, and trichoderma continuously generates a large amount of conidia, antagonistic substances and cellulase under the induction of sugarcane waste molasses or molasses alcohol fermentation liquor, so that the biocontrol effect of the agent is continuously exerted.
Example 2
A method for improving trichoderma application effect of an orchard, particularly a vineyard, comprises the following steps:
(1) the trichoderma harzianum preparation of Baeyer company is mixed with clear water according to the concentration of commercial instruction, and then gamma-polyglutamic acid (final concentration 1000mg/L) and waste sugarcane molasses (final Baume value is 0.5 degree Be) are added.
(2) The suspension is uniformly sprayed on the branches of the grapes, especially the parts covered by the old skins.
(3) When the suspension had not dried, a chitosan borate solution (prepared by dissolving 1g of chitosan having a degree of deacetylation of 80% in 100mL of an aqueous boric acid solution having a concentration of 8g/L and diluting the resulting solution with water to 2 volumes) was sprayed immediately to polymerize chitosan borate with gamma-polyglutamic acid and form a selective antibacterial film on the outer layer.
Example 3
A method for improving trichoderma application effect of an orchard, particularly a vineyard, comprises the following steps:
(1) the trichoderma longibrachiatum preparation purchased from China agricultural microbial strain preservation center is fully and uniformly mixed with clear water according to the concentration and the dosage of commercial instructions, and gamma-polyglutamic acid (the final concentration is 500mg/L) and molasses alcohol fermentation liquor (the final Baume value is 5 DEG Be) are added.
(2) The suspension is uniformly sprayed on the branches of the grapes, especially the parts covered by the old skins.
(3) When the suspension had not dried, a chitosan borate solution (prepared by dissolving 1g of chitosan having a degree of deacetylation of 75% or more in 100mL of an aqueous solution of boric acid having a concentration of 10g/L and diluting the solution with water to 10 volumes) was sprayed immediately to polymerize the chitosan borate with γ -polyglutamic acid and form a selective antibacterial film on the outer layer.
In the existing research, molasses alcohol fermentation liquor or sugarcane waste molasses are mostly used as carbon sources for culturing microorganisms, and the main function is to provide nutrition for the microorganisms and enable cells of the microorganisms to divide and grow in large quantities; at present, no research exists for applying molasses alcohol fermentation liquor or sugarcane waste molasses to promoting growth and formation of trichoderma conidia; according to the invention, the research shows that both molasses alcohol fermentation liquor and sugarcane waste molasses can well promote the growth and formation of trichoderma conidium, and the specific test results are shown in tables 1 and 2.
TABLE 1 influence of molasses alcohol fermentation broth concentration on spore production by Trichoderma
TABLE 2 influence of sugarcane molasses concentration on spore production by Trichoderma
As can Be seen from the data in tables 1 and 2, both the molasses alcohol fermentation liquor and the cane molasses can promote the growth of the conidia of the trichoderma well, and the effect is the best when the concentration is 0.5-5.0 degrees Be.
In the method of the present invention, the mixing and compounding of the components and the application sequence are very important, which directly affects the final application effect of trichoderma, and in order to verify the above effects, the present invention makes the following comparative test I: preparing a commercially available biological pesticide-grade chitosan oligosaccharide product with monosaccharide unit polymerization degree of 2-20 into 10g/L aqueous solution, and spraying the aqueous solution on grape branches; and then, fully and uniformly mixing the commercially available trichoderma viride (same as the example 1) with clear water according to the concentration and the using amount of the commercial instructions, adding molasses alcohol fermentation liquor (the final Baume value is 2 degrees Be), adding chitosan borate solution (same as the example 1), mixing to obtain suspension, and spraying the suspension on the grape branches.
According to the method of the above comparative test I, a liquid film layer can be formed on the grape branches in a short time, but the liquid film is air-dried and hardened within 24 hours after each spraying, and the effect of keeping the liquid film moist for a long time (more than 7 days) cannot be realized as in the present invention. Therefore, the above comparative test method cannot achieve the effect of normally growing trichoderma in the liquid film on the surface of the branches for a long time to produce conidia.
In the method of the present invention, the chitosan borate solution plays an important role, and in order to verify the effect of the chitosan borate solution, the present invention also makes the following comparative test II: substantially the same as in example 1 (experiment No. 3 in table 3), except that: the aqueous solution of boric acid was replaced with an aqueous hydrochloric acid solution (experiment No. 1 in table 3) and an aqueous glacial acetic acid solution (experiment No. 2 in table 3) at the same volume and concentration; specific comparison results are shown in table 3.
TABLE 3
As can be seen from the comparison of the results in Table 3, the chitosan acid salt has poor polymerization ability with gamma-polyglutamic acid after different acids are replaced, and the surface inhibition effect and the growth of trichoderma are not ideal, so that not all the chitosan acid salts can achieve the technical effect of the invention.
In the method of the present invention, gamma-polyglutamic acid plays an important role, and in order to verify the effect of gamma-polyglutamic acid, the present invention also makes the following comparative test III: the same as example 1 except that: the gamma-polyglutamic acid is not added in the step (1). Tests show that a uniform protective film layer structure cannot be formed on grape branches without adding gamma-polyglutamic acid, sprayed liquid medicine is in a wall-hanging downflow form and is air-dried within 24 hours, trichoderma cannot grow on the surfaces of the branches, and conidia cannot be formed.
The invention also makes a test for producing bacteriostatic substances by inducing trichoderma with molasses alcohol fermentation liquor, and the result is shown in figure 1. In FIG. 1, A is a pathogenic bacterium (Botrytis cinerea) which normally grows, C is an inhibitory effect of Trichoderma against the pathogenic bacterium when molasses alcohol fermentation broth is not added, and B and D are inhibitory effects of Trichoderma against the pathogenic bacterium when molasses alcohol fermentation broth is added. The comparison and analysis of the groups in the figure show that the pathogenic bacteria colony of the test group added with the molasses alcohol fermentation liquor is obviously smaller than that of the control group not added with the molasses alcohol fermentation liquor, which shows that the molasses alcohol fermentation liquor can promote the growth of trichoderma and generate bacteriostatic compounds for inhibiting pathogenic bacteria, thereby improving the biocontrol effect of the trichoderma preparation.
The invention also prepares the compound of the gamma-polyglutamic acid and the molasses alcohol fermentation liquor in different proportions, sprays the compound on the grape trunks, and analyzes the surface moisturizing effect of the grape trunks. The air humidity in the vineyard during the test was 85% or more, and the specific results are shown in table 4.
TABLE 4
From table 4, it can be seen that when the air humidity in the vineyard field reaches 85% or more, the experimental groups 3 and 4 show strong moisture absorption performance, and can effectively protect the surface from being wet for 7 days or more, and in the grapeyard in the south, the air humidity reaches 85% in a long period, which is an approximate rate event. The experiments prove that the polyglutamic acid and the molasses alcohol fermentation liquor are combined and compounded for use, the moisturizing effect is obviously enhanced, and the coating is kept in a wet state for a long time after the moisturizing performance is enhanced, so that trichoderma is in a physiological active state, and conidia are continuously generated (if the coating is dry, the trichoderma can be directly killed).
Although the present invention has been described with respect to the preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. A method for improving trichoderma application effect of orchards is characterized by comprising the following steps:
(1) fully and uniformly mixing the trichoderma preparation and clear water, adding gamma-polyglutamic acid and sugarcane waste molasses or adding gamma-polyglutamic acid and molasses alcohol fermentation liquor, and uniformly mixing to obtain a suspension;
(2) uniformly spraying the suspension prepared in the step (1) on the branches of the fruit trees;
(3) and (3) immediately spraying chitosan borate solution when the suspension is not dried, so that the chitosan borate and the gamma-polyglutamic acid are polymerized, and an antibacterial film is formed on the outer layer.
2. The method of claim 1 for increasing trichoderma orchard effectiveness, wherein: the trichoderma is any one of trichoderma viride, trichoderma harzianum and trichoderma longibrachiatum.
3. The method of claim 1 for increasing trichoderma orchard effectiveness, wherein: the orchard is a vineyard, and the fruit tree branches are grape branches.
4. The method of claim 1 for increasing trichoderma orchard effectiveness, wherein: in the step (1), the final concentration of the added gamma-polyglutamic acid is 300-1000 mg/L; the final Baume value of the added sugarcane waste molasses or molasses alcohol fermentation liquor is 0.5-5 DEG Be.
5. The method of claim 1 for increasing trichoderma orchard effectiveness, wherein: in the step (3), the concentration of chitosan in the chitosan boric acid solution is 1-10 g/L.
6. The method of claim 5 for increasing trichoderma orchard effectiveness, wherein: the preparation method of the chitosan boric acid solution comprises the following steps: dissolving 1g of chitosan with deacetylation degree of more than 75% in 100mL of boric acid aqueous solution with concentration of 5-10g/L, and diluting twice according to requirements to obtain the chitosan boric acid solution.
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Application publication date: 20201120 Assignee: GUANGXI WANJIA GRAPE TECHNOLOGY Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046563 Denomination of invention: A Method for Improving the Application Effect of Trichoderma in Orchards Granted publication date: 20211012 License type: Common License Record date: 20231108 |