CN102783502B - Preparation and application of special mildew preventing agent for bamboo wood and bamboo products - Google Patents
Preparation and application of special mildew preventing agent for bamboo wood and bamboo products Download PDFInfo
- Publication number
- CN102783502B CN102783502B CN201210302047.4A CN201210302047A CN102783502B CN 102783502 B CN102783502 B CN 102783502B CN 201210302047 A CN201210302047 A CN 201210302047A CN 102783502 B CN102783502 B CN 102783502B
- Authority
- CN
- China
- Prior art keywords
- bamboo
- mildew
- preparation
- bamboo wood
- molten bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention relates to a preparation and an application of a special mildew preventing agent for bamboo wood and bamboo products. The preparation process comprises the following steps of: using a slant culture medium to activate and culture a lysobacter enzymogenes C3 bacterial strain; transferring the bacterial strain to seed culture liquid; carrying out culturing for 48h at 24-30 DEG C and 230rpm; transferring the bacterial strain to fermentation culture liquid in 10% proportion; and culturing to obtain the mildew preventing agent. The mildew preventing agent can resist a heat treatment for 1h at 70 DEG C or can be stored for more than 180 days without a remarkable activity influence; after the mildew preventing agent treats the bamboo wood in three methods, namely soaking (for more than 4h), uniformly spraying or daubing, a mildew preventing effect is very remarkable; the bamboo wood keeps an original color andperformance. The invention provides a high-efficient biological mildew preventing agent and the application of mildew prevention of the bamboo wood; production cost is low; the conventional microbial fermentation equipment and a technology can be utilized for mass production; and source is very convenient. Mildew-preventing and preservative effects of the bamboo wood is very remarkable by different treatment methods, therefore, the mildew preventing agent has a wide application prospect in preventing mildew and corrosion of the bamboo products.
Description
Technical field
The present invention relates to biological Prevention of Mould Development field, be specifically related to preparation and the application thereof of a kind of bamboo wood and bamboo product special microorganism anti-mildew agent.
Background technology
China is a forest tree resource country in short supply, and finding new timber replacement resources is urgent tasks.The chemical composition of bamboo wood is similar to timber, and three major polymers: cellulose, hemicellulose and lignin form, alternative timber.And the bamboo resource of China is also very abundant, have 39 genus, more than 500 kinds.Bamboo class also has the advantages such as growth is rapid, output is high, become a useful person early, purposes is wide, expands bamboo development and utilizes dynamics, significant to local economic development and ecological construction.Yet bamboo wood is compared with timber containing more starch, polysaccharide and water soluble nutrients.Wherein protein content 1.5%～6.0%, soluble saccharide 2%, starch based 2%～5%, fat and wax class are 2.0%～3.5%.These materials easily go mouldy while making bamboo wood and bamboo product preserve under the environmental condition of warm moist and use, rotten and damage by worms.Gently cause its surface to lose original color and luster, exterior quality declines, and when serious, causes material rotten, and hardness, intensity and serviceability reduction even completely lose.According to estimates, every year because of 10 % (Zhao He rotten, that the bamboo wood that loses of damaging by worms, break accounts for whole world bamboo wood output, open and build, Li Qin. bamboo wood fungus-proof antisepsis present Research and development trend [J]. Shanxi architecture. 2010,36 (29): 137-139).Therefore, research bamboo wood aseptic technic, carries out fungus-proof antisepsis processing to bamboo wood and bamboo product, is the necessary guarantee that makes full use of bamboo timber resource.
Mould harm bamboo wood is by its growth and breeding process implementation, and their spore gives the seed that is equivalent to higher plant, is monokaryon or apocyte, by propagation such as wind, water and insects, also can be propagated by the bamboo wood transportation of being injured.Under suitable temperature and humidity, spore germination forms mycelia, and invade bamboo culm and absorb the sugar growth and breedings such as starch, and then degradation of cell wall cellulose and hemicellulose skeleton, urge and ruin bamboo wood.After fungus growth, conventionally also can produce a large amount of coloured spores or secretion pigment, pollute bamboo material surface, make it to be brown or black, reduce the exterior quality of bamboo wood and bamboo product.For the fungi that causes that bamboo wood goes mouldy, forefathers have done a large amount of basic research: 1994, the Zhao Gui of Nanjing Forestry University China waits the bamboo wood mould (Zhao Guihua that is separated to 11 genus, He Wenlong, Jin Chongwei. the research of Fungi On The Bamboo Wood [J]. Nanjing Forestry University's journal. 1994,18 (3): 87-90); The China Wu of forest-science academy opens the nearly 20 kinds of moulds of the reports such as cloud and causes that mao bamboon material goes mouldy, and wherein 10 kinds is the important mould that causes, as mould in: chain lattice spore (
alternaria alternate), aspergillus flavus (
aspergillus flavus), penicillium chrysogenum (
penicillium chrysogenum), Penicillium citrinum (
p.citrinum) and penicillium funiculosum (
p.funiculosum) etc. (Wu Kaiyun, Weng Yuexia. mao bamboon material causes mould and moulding characteristic [J]. forestry scientific research, 1990,3 (4): 303 ~ 309); More than the 20 kind of moulds such as mould, aspergillus, Mucor, head mold, sickle-like bacteria and the trichothecium roseum on rotten bamboo be mould have been identified in the descriptions such as 1994 Wu Guang of Nian， Zhongnan Forestry Inst. gold, and wherein 12 kinds is the common mouldy bacterium of bamboo wood: penicillium chrysogenum (
p.chrysogenum), Penicillium corylophilum (
p.corylophilum), green mold (
p.digitatum), bread mold (
phizopus nigricans) and aspergillus niger (
aspergillus niger) etc. (Wu Guangjin, Lin Xuejian, Ran Longxian. the evaluation that bamboo wood is mould and the screening of mould inhibitor [J]. economic forest research. 1994,12 (2): 50-55); 2000, Xi'nan College of Forestry Wang Wen waits so long and systematically confirms and reported that kind more than 50 causes the fungi (Wang Wenjiu that bamboo wood goes mouldy rotten, brightness is towards luxuriant, Chen Yuhui etc. Study for Mold and Rot Fungi [J]. and Bamboo research transactions. 2000,19 (4): 26-35).
The growth of its mould will be killed or suppress to the mildew-resistant of bamboo wood and bamboo product, anticorrosion and anti-variable color in fact exactly, and processing method can be divided into physics, chemistry and the different measure such as biological.
Physical method comprises: high temperature sterilization, water seaoning and fumigation etc.High temperature sterilization by toasting, tan by the sun, decatize and the method such as boil and kill mould; Water seaoning is that bamboo wood and bamboo product are placed in flowing water or running water and soak a period of time, its top layer solubility sugar and other nutriments is leached, and cause anaerobic environment, reduces or prevents fungus growth; Fumigation is by bamboo wood firewood sootiness, becomes brown to surface.Easy mildew still after the bamboo wood moisture absorption that first two method is processed, the bamboo wood that a rear method is processed, if dry or the moisture absorption is also easily mouldy not in time.In addition, far infrared, microwave, ultraviolet ray and x-ray etc. also can destroy bioactivator in thalline, kill mould; Regulate the gas composition of bamboo wood storage environment, reduce oxygen content, cause anaerobic condition, mould can not be grown, moth is choked to death, and these physical mildewproof technology are also appeared in the newspapers.
Chemical method comprises: (1) organic medicament has halohydrocarbon, as 1,2-ethylene dibromide and Celfume etc.; Phenol and derivative class thereof, as pentachlorophenol, penta sodium pentachlorophenate, 2, the chloro-3-bromophenol of 5-bis-, 2,4-DNP etc.; Organic phosphates, as letter sulphur phosphorus, phoxim, malathion, acephatemet etc.; Carbamates, as Bassa, the spirit of many poison, unden etc.; Cinerins, as Permanone etc.; Quaternary ammonium salt, as Dodecyl dimethyl benzyl ammonium bromide (bromogeramine) etc.; Nitrile, as tpn etc.; Organo-metallic compound class, as two tributyl tin; Methyllanthionine ester class, as methylene dithiocyanogen acid esters; Carboxylic acid and its esters, as acetic acid, lead acetate, copper naphthenate (or zinc) etc.(2) inorganic medicament has copper sulphate, sodium dichromate, chromium trioxide, arsenic acid hydrogen sodium, pentachloro-ization two arsenic, boric acid, Boratex, four hydration eight Boratexes, mercury oxide, diboron trioxide, sodium fluoride, prodan, zinc chloride, zinc sulphate, mercury chloride, ammoniacal liquor, sulphur etc.
Current most of manufacturing enterprise adopts drying and processing to reduce bamboo product moisture and controls mould, yet bamboo wood has water imbibition, and after the moisture absorption, mould still can grow again, poor effect; Or adopt the medicaments such as industrial soda, hydrogen peroxide, entomophthora spirit to soak.Industrial soda and hydrogen peroxide are short-term mould inhibitor, and effect is general, and are unsuitable for keeping the product of bamboo wood true qualities; Entomophthora spirit has insect protected and mildew-proof function concurrently, but after long-term prescription, induction is developed immunity to drugs, and anti-mold effect falls sharply.The mould happy mould inhibitor (TMO) of dispelling that other chemical anti mildew agents of recent domestic development mainly contain Chinese forest-science academy Tropical Forest Research Institute has anti-mold effect (to execute the seat that shakes to mao bamboon flooring board, Luo Tushou, Li Yun. eight kinds of mould inhibitors are to rubber wood timber and bamboo wood mould and the blue toxicity test [J] that becomes bacterium. timber industry. 1994,8 (3): 34-38); The ACB mould inhibitor of Nanjing Forestry University development can prevent going mouldy and damage by worms (Song Zhen, You Jixue, He Wenlong. ACB preservative is for the test [J] of bamboo wood mildew-resistant. forestry science and technology exploitation. 1994,3:29-30.); The molten alkylamine copper of the Wood protecting agent ammonia (ACQ of U.S. CSI company (Chemical Specialties Inc) development, Alkaline Copper Quaternary), (the Tascioglu C that comes into operation of the countries such as the Yi U.S., Japan and Southeast Asia, Cooper P, Ung T. Rate and extent of adsorption of ACQ preservative components in wood[J]. Holzforschung. 2005,59 (5): 574-580); Organic iodide 3-iodo-2-propynyl first propylhomoserin butyl ester (IPBC, Iodopropynyl Butyl Carbamate) (Badreshia S respond well in Hawaii, America wood preservation, Marks JG. Iodopropynyl butylcarbamate[J]. American Journal of Contact Dermatitis. 2002,13 (2): 77-79).Also have other water miscible alkylammonium compounds (AAC), the molten quaternary ammonium copper of ammonia (ACQ), boride, two cupric dimethyldithio carbamate (CDDC) and oil-soluble copper naphthenate/zinc, tpn (CTL, Chlorothalonil), organic iodide, pyrethroid etc. (Jiang Mingliang. the Recent Research of novel wood preservative-tpn [J]. timber industry. 1997,11 (004): 21-22).
Conventionally, above-mentioned physical mildewproof change technical operation is simple, Environmental Safety is high, but effect is shorter.Chemical treatment is can longer-term anti-to go mouldy, but processing cost is high and Environmental Safety more not.Development of new is efficient, low toxic and environment-friendly, fungusproof anti-corrosion agent with low cost are still focus and trend.
Biological mould inhibitor is because of to people, animal safety, and can be the Prevention of Mould Development of environmental protection by approach safe disposal such as photodissociation, hydrolysis and enzymolysis, and the non-toxic, environmental friendly biology mould inhibitor that development cost is cheap will be Research Emphasis and new direction.Now carried out the correlative study of the natural polymer products such as chitin, linseed oil, plant extract to bamboo and wood protection both at home and abroad.Because of reasons such as antibacterial effect are undesirable, production cost is high, raw material sources are limited and yield poorly, all fail to realize and apply.
Summary of the invention
Project of the present invention is announced a kind of microorganism anti-mildew agent, can directly adopt existing microorganism fermentation equipment and technology large-scale mass production, produces raw material and can use wide material sources, carbohydrate or agricultural byproducts offcuts with low cost.The molten bacillus of production bacterium product enzyme of this microbial inoculum (
lysobacter enzymogenes) C3 bacterial strain obtained the agricultural safety approval of U.S. FDA, uses safe without toxic side effect, to environment also green safety, mildew-resistant antiseptic effect highly significant after using, low production cost, convenient sources, has production sustainability.
This project with produce the molten bacillus of enzyme (
lysobacter enzymogenes) (American type culture collection, strain number: ATCC29487) anti-mildew agent is made in fermentation, adopts distinct methods to process bamboo wood to C3 bacterial strain, all obtains good anticorrosive mildewproof and becomes effect.This microbial inoculum is safety and environmental protection, the advantage such as with low cost not only, produces and uses also very simple and easy to do.
For realizing the technical scheme of object employing of the present invention, be:
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
On aseptic working platform, the molten bacillus C3 of the product enzyme inoculation of-80 ℃ of preservations is carried out to bacterial strain activation in previously prepared good test tube slant medium, obtain the molten bacillus C3 of the product enzyme bacterial strain of activation.Activation temperature in described bacterial strain activation is 24～30 ℃, and soak time is 36～52h.
Described slant medium formula is peptone: 10g, yeast extract: 5g, and sodium chloride: 10g, agar: 20g, running water: 1L configuration forms; Medium pH 7.0～7.2, by 121 ℃ of sterilizing 20min.
2, the preparation of shake-flask seed liquid
The molten bacillus C3 of the product enzyme bacterial strain that picking has activated from inclined-plane, is transferred in several erlenmeyer flasks that 100ml culture fluid is housed, and 24～30 ℃, 230rpm cultivates 36～52h and is prepared into shake-flask seed liquid.The molten bacillus C3 of the product enzyme bacterial strain that the ring of transferring in each erlenmeyer flask has activated.
Described culture fluid formula is: sucrose 1 g, peptone 1g, yeast extract 0.5g, sodium chloride 1g, running water 100ml; Culture fluid pH 7.0～7.2, by 121 ℃ of sterilizing 20min.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Adopt fermentation tank, inject 60% volume left and right culture fluid, the shake-flask seed liquid of preparing with the inoculum concentration inoculation previous step of culture fluid volume 10%, agitator rotating speed is 230r/min, the speed of the lasting air with half volume of zymotic fluid per minute passes into filtrated air, cultivate 68～82h, make instant and produce the molten bacillus C3 of enzyme anti-mildew agent for direct.
Instant is produced to the molten bacillus C3 of enzyme anti-mildew agent according to different use specification sealing bottlings or sealed cans packing, after packing, unification is heated to 70 ℃, and keep 1h constant temperature to process, and kill the viable bacteria in microbial inoculum, be prepared into storage type and produce the molten bacillus C3 of enzyme anti-mildew agent for lagging behind.70 ℃ and to keep the object that 1h constant temperature is processed be to be beneficial to store and transportation.Culture fluid used in fermentation tank, its formula is identical with culture fluid of shake-flask seed preparation process use.
4, special mildewproof microbial inoculum is in the application of bamboo wood or bamboo product
Fresh bamboo wood is cut into little bamboo chip, adopt immersion, sprinkling or brushing method, utilize the molten bacillus C3 of the product enzyme anti-mildew agent of step 3 or step 4 preparation to process, little bamboo chip anti-mold effect is after treatment good.
When adopting soaking method is processed, little bamboo chip or bamboo product are soaked in and produce the molten bacillus C3 of enzyme anti-mildew agent 4～12 hours, take out little bamboo chip or bamboo product after room temperature dry for further.
While adopting spray method to process, utilize sprayer, the molten bacillus C3 of the product enzyme anti-mildew agent of step 3 or step 4 preparation is evenly sprayed on to the surface of little bamboo chip or bamboo product, room temperature is dried for further.
While adopting brushing method processing, utilize hairbrush, the molten bacillus C3 of the product enzyme anti-mildew agent of step 3 or step 4 preparation is evenly painted on to the surface of little bamboo chip or bamboo product, make little bamboo chip or bamboo product surface completely moist, room temperature is dried for further.
Anti-mildew agent prepared by the present invention, more than can tolerating 70 ℃ of heat treated 1h and room temperature storage 180d, and activity is not made significant difference, adopt and three kinds of modes such as soak, evenly spray or smear and process bamboo wood, the equal highly significant of anti-mold effect, bamboo wood keeps original color and luster and performance completely.The present invention is a kind of efficient biological anti-mildew agent and bamboo wood mildew-resistant application thereof, produces bacterium and produces the safety approval that the molten bacillus C3 of enzyme bacterial strain has obtained U.S. FDA, and the complete green safety of environment is had no side effect.Low production cost, can utilize existing microorganism fermentation equipment and technology to produce in a large number, originate very convenient.Different treatments is to having broad prospect of application on the fungus-proof antisepsis effect highly significant ， bamboo and wood products fungus-proof antisepsis of bamboo wood.
Accompanying drawing explanation
Fig. 1 be on the control treatment bamboo wood described in patent working example 1 of the present invention institute isolated 9 kinds of moulds (strain number is: zhengmei 1-9).
Fig. 2 is that the molten bacillus C3 of the employing instant product enzyme anti-mildew agent described in the embodiment of the present invention 1 is placed the anti-mold effect figure of 180 days naturally by little bamboo chip after immersion treatment.
Fig. 3 be described in patent working example 2 of the present invention on control treatment group bamboo wood 6 kinds of separated mouldy bacterial strains (be numbered: zmei a-f)
Fig. 4 is that the molten bacillus C3 of the employing storage type product enzyme anti-mildew agent described in the embodiment of the present invention 2 is placed the anti-mold effect figure of 60 days naturally by little bamboo chip after immersion treatment.
Fig. 5 is that the employing little bamboo chip after the molten bacillus C3 of the storage type product enzyme anti-mildew agent of storing 180 days is processed described in the embodiment of the present invention 3 is placed the anti-mold effect figure of 60 days naturally.
Fig. 6 is that after the employing spray method described in the embodiment of the present invention 4 is processed, little bamboo chip is placed the anti-mold effect figure of 15 days naturally.
Fig. 7 is that after the employing smearing method described in the embodiment of the present invention 5 is processed, bamboo chip is placed the anti-mold effect figure of 15 days naturally.
Fig. 8 is employing instant, the storage type described in the embodiment of the present invention 6 and places the storage type anti-mildew agent after 180 days, and the separated mouldy bacterial strain of 6 strain bamboo wood (zmei a-f) of the embodiment 2 of take, as for examination mould, is investigated its antibacterial activity result figure.
Fig. 9 is that the molten bacillus C3 of the employing instant product enzyme anti-mildew agent described in the embodiment of the present invention 6 is placed the anti-mold effect figure of 180 days naturally by large stretch of bamboo clappers after immersion treatment.
In Fig. 2, control group adopts sterile water to soak, and processed group adopts instant to produce the immersion of the molten bacillus C3 of enzyme anti-mildew agent, and soak time is 12h.
In Fig. 4, control group adopts sterile water to soak 4h, and processed group adopts the storage type of the preparation of 70 ℃ of heating 1h to produce the molten bacillus C3 of enzyme anti-mildew agent soak time and is 4h.
In Fig. 5, processed group is to adopt storage type to produce the molten bacillus C3 of enzyme anti-mildew agent through 70 ℃ of heat treated 1h, and stores the design sketch using afterwards for 180 days.
In Fig. 6, processed group adopts instant to produce the molten bacillus C3 of enzyme anti-mildew agent, and control group sterile water is moistening to bamboo chip with sprayer surface spray.
In Fig. 7, processed group adopts storage type to produce the molten bacillus C3 of enzyme anti-mildew agent, control group sterile water, and it is moistening that hairbrush surface smears to bamboo chip.
In Fig. 9, control group adopts sterile water to soak, and processed group adopts instant to produce the immersion of the molten bacillus C3 of enzyme anti-mildew agent, and soak time is 12h.
Embodiment
embodiment 1
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
On aseptic working platform by the molten bacillus of product enzyme of-80 ℃ of preservations (
lysobacter enzymogenes) C3 inoculation in previously prepared good test tube slant medium, cultivate 52h for 24 ℃.
Described slant medium formula is peptone: 10g, yeast extract: 5g, and sodium chloride: 10g, agar: 20g, running water: 1L configuration forms; Medium pH 7.0～7.2, by 121 ℃ of sterilizing 20min.
The described molten bacillus C3 of product enzyme bacterial strain is from American type culture collection, strain number: ATCC29487.
2, the preparation of shake-flask seed liquid
The molten bacillus C3 of the product enzyme bacterial strain of picking activation from previous step inclined-plane, is transferred in 4 erlenmeyer flasks that 100ml culture fluid is housed, and 28 ℃, 230rpm cultivates 40h and is prepared into shake-flask seed liquid.The molten bacillus C3 of the product enzyme bacterial strain that the ring of transferring in each erlenmeyer flask has activated.
Described culture fluid formula is: sucrose 1g, peptone 1g, yeast extract 0.5g, sodium chloride 1g, running water 100ml; Culture fluid pH 7.0～7.2, by 121 ℃ of sterilizing 20min.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Adopt 5 L fermentation tanks, inject 3 L culture fluids, 300ml shake-flask seed liquid prepared by previous step is inoculated in shake-flask seed fermentation tank culture liquid, start agitator, the rotating speed of agitator is 230r/min, flow velocity with 1.5L/min continues to pass into filtrated air simultaneously, cultivates 75h, makes instant and produces molten bacillus C 3 anti-mildew agents of enzyme.Culture fluid used, the culture fluid that its formula is used with shake-flask seed preparation process is identical.
4, the mildew-resistant of bamboo wood infusion method is processed
Little bamboo chip by fresh bamboo wood cutting into about 7 * 0.5cm, is divided at random two groups of control group and processed group and carries out 12h immersion control treatment.The sterile water immersion of spending the night for control group, the processed group immersion of spending the night in producing the molten bacillus C3 of enzyme anti-mildew agent.
Soak after 12 hours, respectively control group and processed group bamboo chip are taken out, be positioned in the sterile petri dish of oven dry, room temperature is placed, and makes regular check on fungus growth situation.
Result shows, control group after one week with regard to mildew, and therefrom separated 9 kinds of moulds (No. 1-9, zhengmei, is shown in accompanying drawing 1).And produce the molten bacillus C3 of enzyme anti-mildew agent processed group, do not observe fungus growth, surface still keeps original fresh colour, and anti-mildew agent mould fungus inhibition growth result is remarkable, fungus-proof antisepsis.Place after two months, control group mould raised growth, substantially rotten rotten, and processed group virtual standstill, half a year is above all without marked change.As shown in Figure 2.
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
On aseptic working platform by the molten bacillus C3 of the product enzyme inoculation of-80 ℃ of preservations in previously prepared good test tube slant medium, cultivate 36h for 30 ℃.
Described slant medium formula is identical with embodiment 1.
2, the preparation of shake-flask seed liquid
The molten bacillus C3 of the product enzyme bacterial strain that picking has activated from inclined-plane, is transferred in 7 erlenmeyer flasks that 100ml culture fluid is housed, and 24 ℃, 230rpm cultivates 48h and is prepared into shake-flask seed liquid.The molten bacillus C3 of the product enzyme bacterial strain that the ring of transferring in each erlenmeyer flask has activated.
Described culture fluid formula and sterilization method are identical with embodiment 1.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Adopt 10 L fermentation tanks, inject 6L culture fluid, 600ml shake-flask seed liquid is inoculated in fermentation tank culture liquid, start agitator, agitator rotating speed is 230r/min, continues to pass into filtrated air with 3.0L/min flow velocity simultaneously, cultivate 68h, make instant and produce the molten bacillus C3 of enzyme anti-mildew agent.Culture fluid used, the culture fluid that its formula is used with shake-flask seed preparation process is identical.
4, anti-mildew agent pasteurization is processed
The instant that fermentation is made produces the molten bacillus C3 of enzyme anti-mildew agent and packs in vial according to the use specification of 5kg, unified being positioned in disinfect box after packing, be heated to 70 ℃, and keep 1h constant temperature to process, kill the viable bacteria in anti-mildew agent, the instant of processing through pasteurization produces the molten bacillus C3 of enzyme anti-mildew agent and is converted into the molten bacillus C3 of storage type product enzyme anti-mildew agent, standby.
5, the mildew-resistant of bamboo wood infusion method is processed
Little bamboo chip by fresh bamboo wood cutting into about 7 * 0.5cm, is divided at random two groups of control group and processed group and carries out 4h immersion treatment.Control group soaks with sterile water, and processed group directly adopts the storage type of previous step after 70 ℃ of heating 1h to produce the immersion of the molten bacillus C3 of enzyme anti-mildew agent.
After immersion, respectively control group and processed group bamboo chip are taken out, be positioned in the sterile petri dish of oven dry, room temperature is placed, and makes regular check on fungus growth situation.
Result shows, control group one week is mouldy afterwards, and therefrom isolates 6 kinds of moulds (be numbered: zmei a-f, referring to accompanying drawing 2), and processed group is not observed fungus growth, and bamboo chip surface still keeps original fresh colour, shows that mould fungus inhibition growth result is remarkable.Place after two months, control group mould raised growth, substantially rotten rotten, and processed group virtual standstill is not any change.Result as shown in Figure 4.
embodiment 3
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
Identical with embodiment 1.
2, the preparation of shake-flask seed liquid
Identical with embodiment 1.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Identical with embodiment 2.
4, anti-mildew agent pasteurization is processed
Preparation process is identical with heat treated embodiment 2, and uses afterwards depositing 180 days under prepared anti-mildew agent room temperature.
5, the mildew-resistant of bamboo wood infusion method is processed
Little bamboo chip by fresh bamboo wood cutting into about 7 * 0.5cm, and be divided at random two groups, and control group soaks 4h with sterile water, and processed group is produced the molten bacillus C3 of enzyme anti-mildew agent by the storage type of storing 180 days and is soaked 4 hours, after immersion, take out and be put in the sterile petri dish of oven dry respectively, room temperature is placed.As shown in Figure 5, control group is little bamboo chip mildew after one week for result, and the little bamboo chip of processed group is still without obvious mildew phenomenon.
embodiment 4
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
Activation is identical with embodiment 1 with sterilization method.
2, the preparation of shake-flask seed liquid
The preparation of shake-flask seed liquid is identical with embodiment 1.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent identical with embodiment 1.
4, bamboo wood directly sprays mildew-resistant processing
Little bamboo chip by bamboo wood cutting into about 7 * 0.5cm, and be divided into two groups, control group evenly sprays with sterile water, makes bamboo chip surface completely moist, and the instant anti-mildew agent that processed group is prepared by previous step corresponding to control group sprays.Two groups of bamboo chips are placed on respectively in the corresponding culture dish of sterilized oven dry, are positioned over room temperature, control group is placed after one week and is grown mould, and processed group anti-mold effect is obvious, as shown in Figure 6.
embodiment 5
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
Activation is identical with embodiment 2 with sterilization method.
2, the preparation of shake-flask seed liquid
The preparation of shake-flask seed liquid is identical with embodiment 2.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent identical with embodiment 2.
4, the direct brushing mildew-resistant of bamboo wood is processed
Little bamboo chip by bamboo wood cutting into about 7 * 0.5cm, and be divided into two groups, control group is evenly brushed with sterile water, makes bamboo chip surface completely moist, and the storage type that processed group is prepared by previous step corresponding to control group produces the brushing of the molten bacillus C3 of enzyme anti-mildew agent.Two groups of bamboo chips are placed on respectively in the corresponding culture dish of sterilized oven dry, are positioned over room temperature, control group is placed after one week and is grown mould, and processed group anti-mold effect is obvious equally, as shown in Figure 7.
Embodiment 6
1, the molten bacillus C3 of enzyme bacterial strain is produced in activation.
Identical with embodiment 1.
2, the preparation of shake-flask seed liquid
Identical with embodiment 1.
3, produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Identical with embodiment 2, make instant anti-mildew agent.
4, anti-mildew agent pasteurization is processed
Preparation process is identical with heat treated embodiment 2, and instant anti-mildew agent changes the storage type anti-mildew agent of at room temperature depositing 180 days into.
5, the mouldy bacterial strain of 6 strain bamboo wood that activation embodiment 2 is separated to, gets respectively central authorities' (being labeled as respectively A, B, C, D, E, F with corresponding zmei a-f bacterial strain) that a fritter is inoculated into corresponding PDA plating medium.Then in each dull and stereotyped bacterial strain surrounding, put 4 sterilized Oxford cups respectively, every glass adds respectively 200ul sample: be for No. 1 sterile water (contrast); It is for No. 2 instant anti-mildew agent; It is for No. 3 storage type anti-mildew agent; No. 4 for depositing the storage type anti-mildew agent after 180 days.Be placed in 28 ℃ of cultivations, until visible clear inhibition zone, as shown in Figure 8.
6, the mildew-resistant of bamboo board infusion method is processed
Two of large stretch of bamboo boards getting 60 * 20cm, are divided into control group and processed group at random, and two groups are carried out 12h contrast immersion treatment.Control group soaks with sterile water, and processed group is produced the molten bacillus C3 of enzyme anti-mildew agent with instant and soaked.
Soak after 12 hours, respectively control group and processed group bamboo clappers are taken out, room temperature is placed, and checks mouldy situation.
Place after 60 days, control group bamboo clappers surfaces externally and internally all has mould raised growth, starts mould rotten variable color, and processed group virtual standstill extends and places half a year above all without marked change, keeps original color and luster, as shown in Figure 9.
Claims (10)
1. a preparation for bamboo wood and bamboo product special mildewproof microbial inoculum, is characterized in that:
1) the molten bacillus C3 of enzyme bacterial strain is produced in activation
On aseptic working platform, the molten bacillus C3 of the product enzyme inoculation of-80 ℃ of preservations is carried out to bacterial strain activation in previously prepared good test tube slant medium;
2) preparation of shake-flask seed liquid
The C3 bacterial strain that picking has activated from inclined-plane, is transferred to and in the erlenmeyer flask that culture fluid is housed, carries out shaking flask and cultivate and be prepared into shake-flask seed liquid;
3) produce the preparation of the molten bacillus C3 of enzyme anti-mildew agent
Adopt fermentation tank, inject 60% volume left and right culture fluid, the shake-flask seed liquid of preparing with the inoculum concentration inoculation previous step of culture fluid volume 10% is cultivated, and makes instant and produces the molten bacillus C3 of enzyme anti-mildew agent;
Instant is produced to the molten bacillus C3 of enzyme anti-mildew agent and according to different use specifications, seal bottling or sealed cans packing, after packing, unification is heated to 70 ℃, and keeps 1h constant temperature sterilization processing, is prepared into storage type and produces the molten bacillus C3 of enzyme anti-mildew agent;
Described seed liquor and zymotic fluid culture medium prescription are: sucrose 1g, peptone 1g, yeast extract 0.5g, sodium chloride 1g, running water 100ml; Medium pH 7.0～7.2, by 121 ℃ of sterilizing 20min.
2. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum, is characterized in that described bacterial strain activation, and activation temperature is 24～30 ℃, and soak time is 36～52h.
3. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum, is characterized in that described slant medium formula is peptone: 10g, yeast extract: 5g, and sodium chloride: 10g, agar: 20g, running water: 1L configuration forms.
4. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum, is characterized in that described shaking flask cultivation, and cultivation temperature is 24～30 ℃, and shaking flask speed is 230rpm, and incubation time is 36～52h.
5. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum, it is characterized in that the C3 bacterial strain that described picking has activated, be transferred to and in erlenmeyer flask, carry out shaking flask while cultivating, the C3 bacterial strain that the ring of transferring in each erlenmeyer flask has activated.
6. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum, while it is characterized in that described employing fermentation tank culture, agitator rotating speed is 230r/min, the speed of the lasting air with half volume of zymotic fluid per minute passes into filtrated air, incubation time 68～82h simultaneously.
7. the bamboo wood that prepared by a preparation method as claimed in claim 1 and the application of bamboo product special mildewproof microbial inoculum, it is characterized in that fresh bamboo wood to cut into little bamboo chip, adopt immersion, sprinkling or brushing method, utilize the molten bacillus C3 of the product enzyme anti-mildew agent of claim 1 preparation to carry out mildew-resistant processing.
8. the application of the bamboo wood that prepared by preparation method as claimed in claim 7 and bamboo product special mildewproof microbial inoculum, it is characterized in that described immersion process, that little bamboo chip or bamboo product are soaked in and produce the molten bacillus C3 of enzyme anti-mildew agent 4～1 2 hours, take out little bamboo chip or bamboo product after room temperature dry for further.
9. the application of the bamboo wood that prepared by preparation method as claimed in claim 7 and bamboo product special mildewproof microbial inoculum, it is characterized in that described spray method, to utilize sprayer, the molten bacillus C3 of product enzyme anti-mildew agent is evenly sprayed on to the surface of little bamboo chip or bamboo product, make little bamboo chip or bamboo product surface completely moist, room temperature is dried for further.
10. the application of the bamboo wood that prepared by preparation method as claimed in claim 7 and bamboo product special mildewproof microbial inoculum, it is characterized in that described brushing method, to utilize hairbrush, the molten bacillus C3 of product enzyme anti-mildew agent is evenly painted on to the surface of little bamboo chip or bamboo product, make little bamboo chip or bamboo product surface completely moist, room temperature is dried for further.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210302047.4A CN102783502B (en) | 2012-08-22 | 2012-08-22 | Preparation and application of special mildew preventing agent for bamboo wood and bamboo products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210302047.4A CN102783502B (en) | 2012-08-22 | 2012-08-22 | Preparation and application of special mildew preventing agent for bamboo wood and bamboo products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102783502A CN102783502A (en) | 2012-11-21 |
| CN102783502B true CN102783502B (en) | 2014-02-19 |
Family
ID=47149265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210302047.4A Active CN102783502B (en) | 2012-08-22 | 2012-08-22 | Preparation and application of special mildew preventing agent for bamboo wood and bamboo products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102783502B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104070573B (en) * | 2014-06-17 | 2016-01-27 | 福建农林大学 | A kind of anti-mildew method of bamboo wood of high-efficiency environment friendly |
| CN104388351B (en) * | 2014-11-24 | 2018-02-16 | 福州科力恩生物科技有限公司 | A kind of natural preparation method without colour biological fungus-proof antisepsis microbial inoculum |
| CN104441182A (en) * | 2014-12-12 | 2015-03-25 | 如皋市东陈镇爱红工艺品厂 | Novel antibacterial anti-mildew bamboo carving handicraft manufacturing method |
| CN105685105A (en) * | 2016-03-25 | 2016-06-22 | 福建师范大学 | Method for preparing high-protein bacterial powder by utilizing antibiotic fungus residues |
| CN106434439A (en) * | 2016-09-21 | 2017-02-22 | 云南农业大学 | Lysobacter enzymogenes 1-T-1-4 and application thereof |
| CN106493826B (en) * | 2016-10-07 | 2019-05-10 | 梁志全 | A kind of preparation method of anti-bacterial fibre plate |
| CN108356944A (en) * | 2018-02-10 | 2018-08-03 | 张芸 | A kind of preparation method of wood-based plate mould inhibitor |
| CN109049251A (en) * | 2018-08-22 | 2018-12-21 | 浙江恋家生活家居有限公司 | A kind of processing technology of deformation-preventing wooden door |
| CN110436456A (en) * | 2019-07-08 | 2019-11-12 | 黄山市黟县宏联竹制工艺有限公司 | A kind of production technology of shuttle shape environmental protection bamboo stick |
| CN111671777A (en) * | 2020-06-30 | 2020-09-18 | 福州科力恩生物科技有限公司 | Microbial disinfectant, and preparation method and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MY137123A (en) * | 1993-08-24 | 2008-12-31 | Uniroyal Chem Co Inc | Wood preservative oxathiazines |
| KR100757298B1 (en) * | 2006-01-09 | 2007-09-11 | 순천대학교 산학협력단 | Antagonistic microorganisms having activity against plant pathogens and plant diseases controlling agent comprising the same |
-
2012
- 2012-08-22 CN CN201210302047.4A patent/CN102783502B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102783502A (en) | 2012-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102783502B (en) | Preparation and application of special mildew preventing agent for bamboo wood and bamboo products | |
| CN101932245B (en) | The protective layer of plant and trees, their generation and their purposes | |
| CN102050673B (en) | Preparation method of edible mushroom wood plant mother culture medium and product thereof | |
| CN102726454B (en) | Biological control agent for fusarium wilt and preparation and use method of biological control agent | |
| CN109699682A (en) | A kind of tomato biological and ecological methods to prevent plant disease, pests, and erosion seed coat agent and its application | |
| CN106497831A (en) | A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation method and application | |
| CN103952329A (en) | Bacillus vallismortis and application thereof | |
| CN108148794A (en) | A kind of the bacillus subtilis DYr3.3 and preparation method and application of broad-spectrum antibacterial activity | |
| CN101919441A (en) | Biological antiseptic preservative for controlling fruit penicilliosis | |
| CN108559719B (en) | Liquid microbial fertilizer | |
| CN102630534B (en) | Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes | |
| CN103667079B (en) | A kind of Yi Shi kills line fungus production technology and preparation thereof and process units | |
| CN103086801B (en) | Specific liquid fertilizer for beet planting and manufacture method thereof | |
| CN110713934A (en) | Edible fungus production strain preservation method and activation method thereof | |
| Schoeman et al. | The development of ideas in biological control applied to forest products | |
| CN106615071B (en) | Application of pterostilbene in prevention and treatment of grape gray mold and downy mildew of litchi | |
| CN102160572B (en) | Tangerine biological antistaling agent based on activity of rhodosporidium and fruit elicitor | |
| CN104351259A (en) | Biological control method of grape cankers | |
| CN102648713B (en) | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein | |
| CN107916237B (en) | Photobacterium Hb1029 and application thereof | |
| CN102586136A (en) | Microbial inoculant for improving disease resistance and promoting growth of plants | |
| Liu | Bamboo timber mildew and anti-mold technology | |
| WO2023272996A1 (en) | Nano-silver composite bacillus microbial agent for preventing and treating pestalotiopsis fruit rot and anthracnose of wax apple, and preparation method therefor | |
| CN104742208A (en) | Antiseptic and mildew-proof agent for wooden and/or bamboo product and application thereof | |
| CN105360284B (en) | A kind of biology source fruit and vegetable fresh-keeping agent and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160603 Address after: Minhou County of Fuzhou City, Fujian province 350001 Street Town Road No. 10 middle Technology Research Center 11 floor room 1104 Patentee after: Fuzhou Keli en Biological Technology Co. Ltd. Address before: 350007 Fuzhou Road, Cangshan District, Fujian,, China Patentee before: Fujian Normal University |