CN102783502A - Preparation and application of special mildew preventing agent for bamboo wood and bamboo products - Google Patents
Preparation and application of special mildew preventing agent for bamboo wood and bamboo products Download PDFInfo
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- CN102783502A CN102783502A CN2012103020474A CN201210302047A CN102783502A CN 102783502 A CN102783502 A CN 102783502A CN 2012103020474 A CN2012103020474 A CN 2012103020474A CN 201210302047 A CN201210302047 A CN 201210302047A CN 102783502 A CN102783502 A CN 102783502A
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- bamboo wood
- enzyme
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- Chemical And Physical Treatments For Wood And The Like (AREA)
Abstract
The invention relates to a preparation and an application of a special mildew preventing agent for bamboo wood and bamboo products. The preparation process comprises the following steps of: using a slant culture medium to activate and culture a lysobacter enzymogenes C3 bacterial strain; transferring the bacterial strain to seed culture liquid; carrying out culturing for 48h at 24-30 DEG C and 230rpm; transferring the bacterial strain to fermentation culture liquid in 10% proportion; and culturing to obtain the mildew preventing agent. The mildew preventing agent can resist a heat treatment for 1h at 70 DEG C or can be stored for more than 180 days without a remarkable activity influence; after the mildew preventing agent treats the bamboo wood in three methods, namely soaking (for more than 4h), uniformly spraying or daubing, a mildew preventing effect is very remarkable; the bamboo wood keeps an original color andperformance. The invention provides a high-efficient biological mildew preventing agent and the application of mildew prevention of the bamboo wood; production cost is low; the conventional microbial fermentation equipment and a technology can be utilized for mass production; and source is very convenient. Mildew-preventing and preservative effects of the bamboo wood is very remarkable by different treatment methods, therefore, the mildew preventing agent has a wide application prospect in preventing mildew and corrosion of the bamboo products.
Description
Technical field
The present invention relates to biological mildew-resistant technical field, be specifically related to the preparation and the application thereof of a kind of bamboo wood and bamboo product special microorganism anti-mildew agent.
Background technology
China is a forest tree resource country in short supply, and seeking new timber replacement resources is urgent tasks.The chemical composition of bamboo wood is similar to timber, mainly is made up of alternative timber cellulose, hemicellulose and lignin.And the bamboo resource of China is also very abundant, has 39 genus, more than 500 kind.The bamboo class also has advantages such as growth is rapid, output is high, become a useful person early, purposes is wide, enlarges bamboo development and utilizes dynamics, and is significant to local economic development and ecological construction.Yet bamboo wood is compared with timber and is contained more starch, polysaccharide and water soluble nutrients.Wherein protein content 1.5%～6.0%, soluble saccharide 2%, starch based 2%～5%, fat and wax class are 2.0%～3.5%.These materials make bamboo wood and bamboo product under the environmental condition of warm moist, preserve and be prone to when using go mouldy, rotten and damage by worms.Gently then cause its surface to lose original color and luster, exterior quality descends, and causes material rotten when serious, and hardness, intensity and serviceability reduce even completely lose.According to estimates, annual because of 10 % rotten, that the bamboo wood that loses of damaging by worms, break accounts for whole world bamboo wood output (Zhao He opens and builds Li Qin. bamboo wood fungus-proof antisepsis present Research and development trend [J]. Shanxi architecture. 2010,36 (29): 137-139).Therefore, research bamboo wood aseptic technic is carried out the fungus-proof antisepsis processing to bamboo wood and bamboo product, is the necessary guarantee that makes full use of bamboo timber resource.
Mould harm bamboo wood realizes that through its growth and breeding process their spore gives the seed that is equivalent to higher plant, is monokaryon or apocyte, through propagation such as wind, water and insects, also can be propagated by the bamboo wood transportation of being injured.Under suitable temperature and humidity, spore germination forms mycelia, and invade the bamboo wood cell and absorb sugar growth and breedings such as starch, and then degradation of cell wall cellulose and hemicellulose skeleton, urge and ruin bamboo wood.Usually also can produce a large amount of coloured spores or secretion pigment behind the fungus growth, pollute the bamboo wood surface, make it to be brown or black, reduce the exterior quality of bamboo wood and bamboo product.For the fungi that causes that bamboo wood goes mouldy; Forefathers have done a large amount of basic research: 1994; Nanjing Forestry University Zhao Gui China etc. is separated to the bamboo wood mould (Zhao Guihua of 11 genus; He Wenlong, Jin Chongwei. the research of bamboo wood fungi [J]. Nanjing Forestry University's journal. 1994,18 (3): 87-90); The China Wu of forest-science academy opens the nearly 20 kinds of moulds of report such as cloud and causes that the mao bamboon material goes mouldy, and wherein 10 kinds is the important mould that causes, as: chain lattice spore mould (
Alternaria alternate), aspergillus flavus (
Aspergillus flavus), penicillium chrysogenum (
Penicillium chrysogenum), Penicillium citrinum (
P.citrinum) and penicillium funiculosum (
P.funiculosum) wait (Wu Kaiyun, Weng Yuexia. the mao bamboon material causes mould and causes mould characteristic [J]. forestry scientific research, 1990,3 (4): 303 ~ 309); 1994, more than 20 kind of moulds such as mould, aspergillus, Mucor, head mold, sickle-like bacteria and the trichothecium roseum on the mashed bamboo be mould were identified in descriptions such as the Wu Guang of Zhongnan Forestry Inst. gold, and wherein 12 kinds is the common mouldy bacterium of bamboo wood: penicillium chrysogenum (
P.chrysogenum), Penicillium corylophilum (
P.corylophilum), green mold (
P.digitatum), bread mold (
Phizopus nigricans) and aspergillus niger (
Aspergillus niger) wait (Wu Guangjin, Lin Xuejian, Ran Longxian. evaluation that bamboo wood is mould and the screening of mould inhibitor [J]. economic forest research. 1994,12 (2): 50-55); 2000, Xi'nan College of Forestry Wang Wen wait so long systematically confirm and reported kind more than 50 cause bamboo wood go mouldy rotten fungi (Wang Wenjiu, brightness be towards luxuriant, Chen Yuhui etc. Study for Mold and Rot Fungi [J]. bamboo research transactions. 2000,19 (4): 26-35).
The mildew-resistant of bamboo wood and bamboo product, anticorrosion and anti-variable color will kill or suppress the growth of its mould in fact exactly, and processing method can be divided into different measure such as physics, chemistry and biology.
Physical method comprises: high temperature sterilization, water seaoning and fumigation etc.High temperature sterilization through toast, tan by the sun, decatize and method such as boil and kill mould; Water seaoning is bamboo wood and bamboo product to be placed in flowing water or the running water soak a period of time, its top layer solubility sugar and other nutriments is leached, and cause anaerobic environment, reduces or prevents fungus growth; Fumigation is that bamboo wood is used the firewood sootiness, becomes brown getting final product to the surface.Still be prone to mildew after the bamboo wood moisture absorption that preceding two kinds of methods are handled, the bamboo wood that back one method is handled, mouldy if the untimely drying or the moisture absorption also are prone to.In addition, far infrared, microwave, ultraviolet ray and x-ray etc. also can destroy bioactivator in the thalline, kill mould; Regulate the gas composition of bamboo wood storage environment, reduce oxygen content, cause anaerobic condition, mould can not be grown, moth is choked to death, and these physical mildewproof technology are also appeared in the newspapers.
Chemical method comprises: (1) organic medicament has halohydrocarbon, like glycol dibromide and Celfume etc.; Phenol and derivatives class thereof, like pentachlorophenol, penta sodium pentachlorophenate, 2,5-two chloro-3-bromophenols, 2,2, 4-dinitrophenol etc.; Organic phosphates is like letter sulphur phosphorus, phoxim, malathion, acephatemet etc.; Carbamates is like Bassa, the spirit of many poison, unden etc.; Cinerins is like Permanone etc.; Quaternary ammonium salt is like bromination dodecyl dimethyl benzyl ammonium (bromogeramine) etc.; Nitrile is like tpn etc.; The organo-metallic compound class is like two tributyl tin; Methyllanthionine ester class is like methylene dithiocyanogen acid esters; Carboxylic acid and its esters are like acetic acid, lead acetate, copper naphthenate (or zinc) etc.(2) inorganic medicament has copper sulphate, sodium dichromate, chromium trioxide, arsenic acid hydrogen sodium, pentachloro-ization two arsenic, boric acid, Boratex, four hydrations, eight Boratexes, mercury oxide, diboron trioxide, sodium fluoride, prodan, zinc chloride, zinc sulphate, mercury chloride, ammoniacal liquor, sulphur etc.
Current most of manufacturing enterprise adopts oven dry to handle reduction bamboo product moisture and controls mould, yet bamboo wood has water imbibition, and mould still can grow after the moisture absorption again, poor effect; Or adopt medicaments such as industrial soda, hydrogen peroxide solution, entomophthora spirit to soak.Industrial soda and hydrogen peroxide solution are the short-term mould inhibitor, and effect is general, and are inappropriate for the product that needs to keep the bamboo wood true qualities; The entomophthora spirit has insect protected and mildew-proof function concurrently, develops immunity to drugs but induce behind the long-term prescription, and anti-mold effect falls sharply.The mould happy mould inhibitor (TMO) of dispelling that other chemical anti mildew agents of developing both at home and abroad in recent years mainly contain Chinese forest-science academy Tropical Forest Research Institute has anti-mold effect (to execute the seat that shakes to the mao bamboon flooring board; Luo Tushou; Li Yun. eight kinds of mould inhibitors are to the toxicity test [J] of rubber wood timber and bamboo wood mould and blue stain bacterium. timber industry. 1994,8 (3): 34-38); The ACB mould inhibitor of Nanjing Forestry University development can prevent going mouldy and damage by worms (Song Zhen, You Jixue, He Wenlong. the ACB preservative is used for the test [J] of bamboo wood mildew-resistant. the forestry science and technology exploitation. 1994,3:29-30.); The Wood protecting agent ammonia of U.S. CSI company (Chemical Specialties Inc) development dissolves alkylamine copper (ACQ; Alkaline Copper Quaternary); At (the Tascioglu C that comes into operation of countries such as the U.S., Japan and Southeast Asia; Cooper P, Ung T. Rate and extent of adsorption of ACQ preservative components in wood [J]. Holzforschung. 2005,59 (5): 574-580); Organic iodide 3-iodo-2-propynyl first propylhomoserin butyl ester (IPBC; Iodopropynyl Butyl Carbamate) (Badreshia S respond well in the Hawaii, America wood preservation; Marks JG. Iodopropynyl butylcarbamate [J]. American Journal of Contact Dermatitis. 2002,13 (2): 77-79).Also have other water miscible alkylammonium compounds (AAC), ammonia to dissolve quaternary ammonium copper (ACQ), boride, two cupric dimethyldithio carbamate (CDDC) and oil-soluble copper naphthenate/zinc, tpn (CTL; Chlorothalonil), organic iodide, pyrethroid etc. (Jiang Mingliang. the research recent developments [J] of novel wood preservative-tpn. timber industry. 1997,11 (004): 21-22).
Usually, above-mentioned physical mildewproof becomes technological simple to operate, Environmental Safety property height, but effect is shorter.Chemical agent handles that but longer-term is anti-to go mouldy, but processing cost is high and Environmental Safety more not.Development of new is efficient, low toxic and environment-friendly, fungusproof anti-corrosion agent with low cost are still focus and trend.
Biological mould inhibitor is because of to people, animal safety, and can be the mildew-resistant technology of environmental protection through approach safe disposal such as photodissociation, hydrolysis and enzymolysis, and the non-toxic, environmental friendly biology mould inhibitor that development cost is cheap will be to research and develop emphasis and new direction.Carried out of the correlative study of natural polymer products such as chitin, linseed oil, plant extract both at home and abroad to the bamboo and wood protection at present.Reason such as undesirable because of antibacterial effect, that production cost is high, raw material sources are limited and yield poorly all fails to realize applying.
Summary of the invention
Project of the present invention is announced a kind of microorganism anti-mildew agent, can directly adopt existing microbial fermentation equipment and technological large-scale mass production, produces raw material and can use wide material sources, carbohydrate or agricultural byproducts offcuts with low cost.The molten bacillus of production bacterium product enzyme of this microbial inoculum (
Lysobacter enzymogenes) the C3 bacterial strain obtained the agricultural safety approval of U.S. FDA, safe in utilization having no side effect to environment also green safety, used back mildew-resistant antiseptic effect highly significant, low production cost, convenient sources, has the production sustainability.
This project with produce enzyme dissolve bacillus (
Lysobacter enzymogenes) (American type culture collection, strain number: ATCC29487) anti-mildew agent is processed in fermentation to the C3 bacterial strain, adopts distinct methods to handle bamboo wood, all obtains good anticorrosion mildew-resistant allergic effect and uses effect.This microbial inoculum is safety and environmental protection, advantage such as with low cost not only, produces and uses also very simple and easy to do.
For realizing that the technical scheme that the object of the invention adopts is:
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
On aseptic workbench, the molten bacillus C3 inoculation of the product enzyme of-80 ℃ of preservations is carried out the bacterial strain activation in the test tube slant medium for preparing in advance, the product enzyme that obtains activation dissolves bacillus C3 bacterial strain.Activation temperature in the described bacterial strain activation is 24～30 ℃, and soak time is 36～52h.
Described slant culture based formulas is peptone: 10g, yeast extract: 5g, and sodium chloride: 10g, agar: 20g, running water: 1L configuration forms; Medium pH 7.0～7.2 is after 121 ℃ of sterilization 20min.
2, the preparation of shake-flask seed liquid
From the inclined-plane picking the product enzyme of activation dissolve bacillus C3 bacterial strain, be transferred in several erlenmeyer flasks that 100ml culture fluid is housed, 24～30 ℃, 230rpm cultivates 36～52h and is prepared into shake-flask seed liquid.Switching one encircles the molten bacillus C3 bacterial strain of product enzyme of activation in each erlenmeyer flask.
Described culture fluid prescription is: sucrose 1 g, peptone 1g, yeast extract 0.5g, sodium chloride 1g, running water 100ml; Culture fluid pH 7.0～7.2 is after 121 ℃ of sterilization 20min.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
Adopt fermentation tank; Inject 60% volume left and right sides culture fluid; With the shake-flask seed liquid that a last step of the inoculum concentration inoculation of culture fluid volume 10% prepares, the agitator rotating speed is 230r/min, continues the speed feeding filtrated air with the air of half volume of per minute zymotic fluid; Cultivate 68～82h, process instant and produce direct use of the molten bacillus C3 anti-mildew agent confession of enzyme.
Instant is produced enzyme dissolve bacillus C3 anti-mildew agent according to different use specification sealing bottlings or envelope jar packing; The packing back is unified to be heated to 70 ℃; And keep the processing of 1h constant temperature, and kill the viable bacteria in the microbial inoculum, be prepared into the molten bacillus C3 anti-mildew agent of storage type product enzyme and supply the use that lags behind.70 ℃ and the purpose that keeps 1h constant temperature to handle are to be beneficial to store and transportation.Used culture fluid in the fermentation tank, its prescription is identical with the culture fluid that shake-flask seed prepares the process use.
4, the special mildewproof microbial inoculum is in the application of bamboo wood or bamboo product
Fresh bamboo wood is cut into little bamboo chip, adopt immersion, sprinkling or brushing method, utilize the molten bacillus C3 anti-mildew agent of product enzyme of step 3 or step 4 preparation to handle, little bamboo chip anti-mold effect after treatment is good.
When adopting soaking method is handled, little bamboo chip or bamboo product be soaked in produce enzyme and dissolved bacillus C3 anti-mildew agent 4～12 hours, take out behind little bamboo chip or the bamboo product room temperature and dry and supply further to use.
When adopting spray method to handle, utilize sprayer, the product enzyme of step 3 or step 4 preparation is dissolved the surface that bacillus C3 anti-mildew agent evenly is sprayed on little bamboo chip or bamboo product, room temperature is dried and is supplied further to use.
When adopting brushing method to handle, utilize hairbrush, the product enzyme of step 3 or step 4 preparation is dissolved the surface that bacillus C3 anti-mildew agent evenly is painted on little bamboo chip or bamboo product, make that little bamboo chip or bamboo product surface is moist fully get final product, room temperature is dried and is supplied further use.
The anti-mildew agent of the present invention's preparation; Can tolerate more than 70 ℃ of heat treated 1h and the room temperature storage 180d, and activity do not made significant difference, adopt and three kinds of modes such as soak, evenly spray or smear and handle bamboo wood; The equal highly significant of anti-mold effect, bamboo wood keeps original color and luster and performance fully.The present invention is that a kind of biological efficiently anti-mildew agent and bamboo wood mildew-resistant thereof are used, and produces bacterium and produces the safety approval that the molten bacillus C3 bacterial strain of enzyme has obtained U.S. FDA, and the complete green safety of environment is had no side effect.Low production cost, existing microbial fermentation equipment capable of using and technology are a large amount of produces, and it is very convenient to originate.The different disposal mode has broad prospect of application to the fungus-proof antisepsis effect highly significant of bamboo wood on bamboo and wood products's fungus-proof antisepsis.
Description of drawings
Fig. 1 be on the patent working of the present invention example 1 described control treatment bamboo wood the isolated 9 kinds of moulds of institute (strain number is: zhengmei 1-9).
Fig. 2 is that the molten bacillus C3 anti-mildew agent of the embodiment of the invention 1 described employing instant product enzyme is placed 180 days anti-mold effect figure naturally through little bamboo chip after the immersion treatment.
Fig. 3 is that 6 kinds of mouldy bacterial strains that separated on the patent working instance 2 said control treatment group bamboo woods of the present invention (are numbered: zmei a-f)
Fig. 4 is that the molten bacillus C3 anti-mildew agent of the embodiment of the invention 2 described employing storage types product enzymes is placed 60 days anti-mold effect figure naturally through little bamboo chip after the immersion treatment.
Fig. 5 is that the embodiment of the invention 3 described employings little bamboo chip after the storage type of storing 180 days produces enzyme to dissolve the processing of bacillus C3 anti-mildew agent is placed 60 days anti-mold effect figure naturally.
Fig. 6 is that little bamboo chip was placed 15 days anti-mold effect figure naturally after the embodiment of the invention 4 described employing spray methods were handled.
Fig. 7 is that bamboo chip was placed 15 days anti-mold effect figure naturally after the embodiment of the invention 5 described employing smearing methods were handled.
Fig. 8 is the embodiment of the invention 6 described employing instants, storage type and places the storage type anti-mildew agent after 180 days that the mouldy bacterial strain of 6 strain bamboo woods (zmei a-f) that is separated with embodiment 2 serves as to supply the examination mould, investigates its antibacterial activity figure as a result.
Fig. 9 is that the molten bacillus C3 anti-mildew agent of the embodiment of the invention 6 described employing instants product enzymes is placed 180 days anti-mold effect figure naturally through large stretch of bamboo clappers after the immersion treatment.
Among Fig. 2, control group adopts sterile water to soak, and processed group adopts the molten bacillus C3 anti-mildew agent of instant product enzyme to soak, and soak time is 12h.
Among Fig. 4, control group adopts sterile water to soak 4h, and processed group adopts the storage type product enzyme of the preparation of 70 ℃ of heating 1h to dissolve bacillus C3 anti-mildew agent soak time and is 4h.
Among Fig. 5, processed group is to adopt the storage type to produce enzyme to dissolve bacillus C3 anti-mildew agent through 70 ℃ of heat treated 1h, and stores the design sketch that uses after 180 days.
Among Fig. 6, processed group adopts instant to produce the molten bacillus C3 anti-mildew agent of enzyme, and control group is used sterile water, and is moistening with sprayer surface spray to bamboo chip.
Among Fig. 7, processed group adopts the storage type to produce the molten bacillus C3 anti-mildew agent of enzyme, and control group is used sterile water, and it is moistening that the hairbrush surface smears to bamboo chip.
Among Fig. 9, control group adopts sterile water to soak, and processed group adopts the molten bacillus C3 anti-mildew agent of instant product enzyme to soak, and soak time is 12h.
Embodiment
Embodiment 1
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
On aseptic workbench with the product enzyme of-80 ℃ of preservations dissolve bacillus (
Lysobacter enzymogenes) the C3 inoculation in the test tube slant medium for preparing in advance, cultivate 52h for 24 ℃.
Described slant culture based formulas is peptone: 10g, yeast extract: 5g, and sodium chloride: 10g, agar: 20g, running water: 1L configuration forms; Medium pH 7.0～7.2 is after 121 ℃ of sterilization 20min.
Described product enzyme dissolves bacillus C3 bacterial strain from American type culture collection, strain number: ATCC29487.
2, the preparation of shake-flask seed liquid
The product enzyme of picking activation dissolves bacillus C3 bacterial strain from a last step inclined-plane, is transferred in 4 erlenmeyer flasks that the 100ml culture fluid is housed, and 28 ℃, 230rpm cultivates 40h and is prepared into shake-flask seed liquid.Switching one encircles the molten bacillus C3 bacterial strain of product enzyme of activation in each erlenmeyer flask.
Described culture fluid prescription is: sucrose 1g, peptone 1g, yeast extract 0.5g, sodium chloride 1g, running water 100ml; Culture fluid pH 7.0～7.2 is after 121 ℃ of sterilization 20min.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
Adopt 5 L fermentation tanks; Inject 3 L culture fluids, the 300ml shake-flask seed liquid that a last step is prepared is inoculated in the shake-flask seed fermentation tank culture liquid, starts agitator; The rotating speed of agitator is 230r/min; Flow velocity with 1.5L/min continues to feed filtrated air simultaneously, cultivates 75h, processes instant product enzyme and dissolves bacillus C 3 anti-mildew agents.Used culture fluid, its prescription is identical with the culture fluid that shake-flask seed prepares the process use.
4, the mildew-resistant of bamboo wood infusion method is handled
With the little bamboo chip of fresh bamboo wood cutting written treaty 7 * 0.5cm, be divided into two groups of control group and processed group at random and carry out 12h immersion control treatment.Control group is with the sterile water immersion of spending the night, and processed group is dissolved the immersion of spending the night in the bacillus C3 anti-mildew agent producing enzyme.
Soak after 12 hours, respectively control group and processed group bamboo chip are taken out, be positioned in the sterile petri dish of oven dry, room temperature is placed, and makes regular check on the fungus growth situation.
The result shows that control group is mildew after week, and has therefrom separated 9 kinds of moulds (zhengmei 1-9 number, see accompanying drawing 1).Do not observe fungus growth and produce the molten bacillus C3 anti-mildew agent processed group of enzyme, the surface still keeps original fresh colour, and anti-mildew agent mould fungus inhibition growth result is remarkable, fungus-proof antisepsis.Place after two months, control group mould raised growth, rotten basically rotten, and the processed group virtual standstill does not all have marked change more than half a year.As shown in Figure 2.
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
Product enzyme with-80 ℃ of preservations on aseptic workbench dissolves bacillus C3 inoculation in the test tube slant medium for preparing in advance, cultivates 36h for 30 ℃.
Described slant culture based formulas is identical with embodiment 1.
2, the preparation of shake-flask seed liquid
From the inclined-plane picking the product enzyme of activation dissolve bacillus C3 bacterial strain, be transferred in 7 erlenmeyer flasks that the 100ml culture fluid is housed, 24 ℃, 230rpm cultivates 48h and is prepared into shake-flask seed liquid.Switching one encircles the molten bacillus C3 bacterial strain of product enzyme of activation in each erlenmeyer flask.
Described culture fluid prescription is identical with embodiment 1 with sterilization method.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
Adopt 10 L fermentation tanks, inject the 6L culture fluid, the 600ml shake-flask seed liquid is inoculated in the fermentation tank culture liquid; Start agitator, the agitator rotating speed is 230r/min, continues to feed filtrated air with the 3.0L/min flow velocity simultaneously; Cultivate 68h, process instant and produce the molten bacillus C3 anti-mildew agent of enzyme.Used culture fluid, its prescription is identical with the culture fluid that shake-flask seed prepares the process use.
4, the anti-mildew agent pasteurization is handled
The instant that fermentation is processed produces enzyme and dissolves bacillus C3 anti-mildew agent and pack in vial according to the use specification of 5kg; The packing back is unified to be positioned in the disinfect box; Be heated to 70 ℃, and keep 1h constant temperature to handle, kill the viable bacteria in the anti-mildew agent; The molten bacillus C3 anti-mildew agent of handling through pasteurization of instant product enzyme promptly is converted into the molten bacillus C3 anti-mildew agent of storage type product enzyme, and is subsequent use.
5, the mildew-resistant of bamboo wood infusion method is handled
Little bamboo chip with fresh bamboo wood cutting written treaty 7 * 0.5cm is divided into control group and processed group at random and carries out the 4h immersion treatment for two groups.Control group soaks with sterile water, and the storage type after processed group directly adopted a last step through 70 ℃ of heating 1h produces enzyme and dissolves the immersion of bacillus C3 anti-mildew agent.
After the immersion, respectively control group and processed group bamboo chip are taken out, be positioned in the sterile petri dish of oven dry, room temperature is placed, and makes regular check on the fungus growth situation.
The result shows, one week of control group the back mouldy, and therefrom isolate 6 kinds of moulds (be numbered: zmei a-f, referring to accompanying drawing 2), and processed group is not observed fungus growth, the bamboo chip surface still keeps original fresh colour, shows that the mould fungus inhibition growth result is remarkable.Place after two months, control group mould raised growth, rotten basically rotten, and the processed group virtual standstill does not have any variation.The result is as shown in Figure 4.
Embodiment 3
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
Identical with embodiment 1.
2, the preparation of shake-flask seed liquid
Identical with embodiment 1.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
Identical with embodiment 2.
4, the anti-mildew agent pasteurization is handled
The preparation process is identical with heat treated embodiment 2, and uses depositing under the prepared anti-mildew agent room temperature after 180 days.
5, the mildew-resistant of bamboo wood infusion method is handled
Little bamboo chip with fresh bamboo wood cutting written treaty 7 * 0.5cm; And be divided into two groups at random, and control group soaks 4h with sterile water, and processed group is produced enzyme with the storage type of storing 180 days and is dissolved bacillus C3 anti-mildew agent immersion 4 hours; Take out respectively after the immersion and be put in the sterile petri dish of oven dry the room temperature placement.The result is as shown in Figure 5, the little bamboo chip mildew in control group one week back, and the little bamboo chip of processed group does not still have tangible mildew phenomenon.
Embodiment 4
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
Activation is identical with embodiment 1 with sterilization method.
2, the preparation of shake-flask seed liquid
The preparation of shake-flask seed liquid is identical with embodiment 1.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
It is identical with embodiment 1 to produce the molten bacillus C3 anti-mildew agent preparation of enzyme.
4, bamboo wood directly sprays the mildew-resistant processing
Bamboo wood is cut the little bamboo chip of written treaty 7 * 0.5cm, and be divided into two groups, control group evenly sprays with sterile water, makes the bamboo chip surface moist fully, and the instant anti-mildew agent that processed group is used step preparation corresponding to control group sprays.Two groups of bamboo chips are placed on respectively in the corresponding culture dish of the bacterium oven dry of going out, are positioned over room temperature, control group grows mould after placing a week, and the processed group anti-mold effect is obvious, and is as shown in Figure 6.
Embodiment 5
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
Activation is identical with embodiment 2 with sterilization method.
2, the preparation of shake-flask seed liquid
The preparation of shake-flask seed liquid is identical with embodiment 2.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
It is identical with embodiment 2 to produce the molten bacillus C3 anti-mildew agent preparation of enzyme.
4, the mildew-resistant of bamboo wood direct brushing is handled
Bamboo wood is cut the little bamboo chip of written treaty 7 * 0.5cm, and be divided into two groups, control group is evenly brushed with sterile water, makes the bamboo chip surface moist fully, and the storage type product enzyme that processed group is used step preparation corresponding to control group dissolves the brushing of bacillus C3 anti-mildew agent.Two groups of bamboo chips are placed on respectively in the corresponding culture dish of the bacterium oven dry of going out, are positioned over room temperature, control group grows mould after placing a week, and the processed group anti-mold effect is obvious equally, and is as shown in Figure 7.
Embodiment 6
1, the molten bacillus C3 bacterial strain of enzyme is produced in activation.
Identical with embodiment 1.
2, the preparation of shake-flask seed liquid
Identical with embodiment 1.
3, produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
Identical with embodiment 2, process the instant anti-mildew agent.
4, the anti-mildew agent pasteurization is handled
The preparation process is identical with heat treated embodiment 2, and the instant anti-mildew agent changes the storage type anti-mildew agent of at room temperature depositing 180 days into.
5, the mouldy bacterial strain of 6 strain bamboo woods that is separated to of activation embodiment 2 is got central authorities' (being labeled as A, B, C, D, E, F respectively with corresponding zmei a-f bacterial strain) that a fritter is inoculated into corresponding PDA plating medium respectively.Around each dull and stereotyped bacterial strain, put the Oxford cup of 4 bacterium of going out then respectively, every glass adds the 200ul sample respectively: No. 1 is sterile water (contrast); No. 2 is the instant anti-mildew agent; Be storage type anti-mildew agent No. 3; No. 4 for depositing the storage type anti-mildew agent after 180 days.Place 28 ℃ of cultivations, until visible clear inhibition zone, as shown in Figure 8.
6, the mildew-resistant of bamboo board infusion method is handled
Get two of large stretch of bamboo boards of 60 * 20cm, be divided into control group and processed group at random, two groups are carried out 12h contrast immersion treatment.Control group soaks with sterile water, and processed group is produced enzyme with instant and dissolved the immersion of bacillus C3 anti-mildew agent.
Soak after 12 hours, respectively control group and processed group bamboo clappers are taken out, room temperature is placed, and checks mouldy situation.
Place after 60 days, control group bamboo clappers surfaces externally and internally all has the mould raised growth, begins mould rotten variable color, and the processed group virtual standstill, prolongation does not all have marked change more than placing half a year, keeps original color and luster, and is as shown in Figure 9.
Claims (10)
1. the preparation of bamboo wood and bamboo product special mildewproof microbial inoculum is characterized in that:
1) the molten bacillus C3 bacterial strain of enzyme is produced in activation
On aseptic workbench, the molten bacillus C3 inoculation of the product enzyme of-80 ℃ of preservations is carried out the bacterial strain activation in the test tube slant medium for preparing in advance;
2) preparation of shake-flask seed liquid
The picking C3 bacterial strain of activation from the inclined-plane is transferred to and carries out shake-flask culture in the erlenmeyer flask that culture fluid is housed and be prepared into shake-flask seed liquid;
3) produce enzyme and dissolve the preparation of bacillus C3 anti-mildew agent
Adopt fermentation tank, inject 60% volume left and right sides culture fluid, inoculate the shake-flask seed liquid of last step preparation with the inoculum concentration of culture fluid volume 10% and cultivate, process instant and produce the molten bacillus C3 anti-mildew agent of enzyme;
Instant is produced the molten bacillus C3 anti-mildew agent of enzyme pack according to different use specification sealing bottlings or envelope jar, the packing back is unified to be heated to 70 ℃, and maintenance 1h constant temperature sterilization processing, is prepared into storage type product enzyme and dissolves bacillus C3 anti-mildew agent.
2. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum is characterized in that described bacterial strain activation, and activation temperature is 24～30 ℃, and soak time is 36～52h.
3. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum is characterized in that described slant culture based formulas is peptone: 10g, yeast extract: 5g, and sodium chloride: 10g, agar: 20g, running water: 1L configuration forms; Seed liquor and zymotic fluid culture medium prescription are: sucrose 1g, peptone 1g, yeast extract 0.5g, sodium chloride 1g, running water 100ml; Medium pH 7.0～7.2 is after 121 ℃ of sterilization 20min.
4. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum is characterized in that described shake-flask culture, and cultivation temperature is 24～30 ℃, and bottle speed of shaking is 230rpm, and incubation time is 36～52h.
5. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum; It is characterized in that the described picking C3 bacterial strain of activation; Be transferred to when carrying out shake-flask culture in the erlenmeyer flask, switching one encircles the C3 bacterial strain of activation in each erlenmeyer flask.
6. the preparation of a kind of bamboo wood according to claim 1 and bamboo product special mildewproof microbial inoculum; When it is characterized in that described employing fermentation tank culture; The agitator rotating speed is 230r/min; Continue speed feeding filtrated air simultaneously, incubation time 68～82h with the air of half volume of per minute zymotic fluid.
7. one kind is utilized the bamboo wood of preparation method's preparation of the present invention and the application of bamboo product special mildewproof microbial inoculum; It is characterized in that fresh bamboo wood is cut into little bamboo chip; Adopt immersion, sprinkling or brushing method, the product enzyme that utilizes the present invention to prepare dissolves bacillus C3 anti-mildew agent and carries out the mildew-resistant processing.
8. a kind of the utilize bamboo wood of preparation method's preparation of the present invention and the application of bamboo product special mildewproof microbial inoculum according to claim 7; It is characterized in that described immersion process; Be little bamboo chip or bamboo product to be soaked in produce enzyme and dissolved bacillus C3 anti-mildew agent 4～1 2 hours, take out behind little bamboo chip or the bamboo product room temperature and dry and supply further to use.
9. a kind of the utilize bamboo wood of preparation method's preparation of the present invention and the application of bamboo product special mildewproof microbial inoculum according to claim 7; It is characterized in that described spray method; Be to utilize sprayer; Dissolve produce enzyme in the surface that bacillus C3 anti-mildew agent evenly is sprayed on little bamboo chip or bamboo product, make complete moist the getting final product in little bamboo chip or bamboo product surface, room temperature is dried and is supplied further to use.
10. a kind of the utilize bamboo wood of preparation method's preparation of the present invention and the application of bamboo product special mildewproof microbial inoculum according to claim 7; It is characterized in that described brushing method; Be to utilize hairbrush; Dissolve produce enzyme in the surface that bacillus C3 anti-mildew agent evenly is painted on little bamboo chip or bamboo product, make complete moist the getting final product in little bamboo chip or bamboo product surface, room temperature is dried and is supplied further to use.
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| CN104070573A (en) * | 2014-06-17 | 2014-10-01 | 福建农林大学 | Efficient and environment-friendly method for preventing bamboo from mildewing |
| CN104388351A (en) * | 2014-11-24 | 2015-03-04 | 福建师范大学 | Preparation method of natural colorless biological mildew-proof anticorrosive bactericide |
| CN104441182A (en) * | 2014-12-12 | 2015-03-25 | 如皋市东陈镇爱红工艺品厂 | Novel antibacterial anti-mildew bamboo carving handicraft manufacturing method |
| CN105685105A (en) * | 2016-03-25 | 2016-06-22 | 福建师范大学 | Method for preparing high-protein bacterial powder by utilizing antibiotic fungus residues |
| CN106434439A (en) * | 2016-09-21 | 2017-02-22 | 云南农业大学 | Lysobacter enzymogenes 1-T-1-4 and application thereof |
| CN106493826A (en) * | 2016-10-07 | 2017-03-15 | 常州创索新材料科技有限公司 | A kind of preparation method of anti-bacterial fibre plate |
| CN108356944A (en) * | 2018-02-10 | 2018-08-03 | 张芸 | A kind of preparation method of wood-based plate mould inhibitor |
| CN109049251A (en) * | 2018-08-22 | 2018-12-21 | 浙江恋家生活家居有限公司 | A kind of processing technology of deformation-preventing wooden door |
| CN110436456A (en) * | 2019-07-08 | 2019-11-12 | 黄山市黟县宏联竹制工艺有限公司 | A kind of production technology of shuttle shape environmental protection bamboo stick |
| CN111671777A (en) * | 2020-06-30 | 2020-09-18 | 福州科力恩生物科技有限公司 | Microbial disinfectant, and preparation method and application thereof |
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|---|---|---|---|---|
| CN104070573A (en) * | 2014-06-17 | 2014-10-01 | 福建农林大学 | Efficient and environment-friendly method for preventing bamboo from mildewing |
| CN104070573B (en) * | 2014-06-17 | 2016-01-27 | 福建农林大学 | A kind of anti-mildew method of bamboo wood of high-efficiency environment friendly |
| CN104388351A (en) * | 2014-11-24 | 2015-03-04 | 福建师范大学 | Preparation method of natural colorless biological mildew-proof anticorrosive bactericide |
| CN104388351B (en) * | 2014-11-24 | 2018-02-16 | 福州科力恩生物科技有限公司 | A kind of natural preparation method without colour biological fungus-proof antisepsis microbial inoculum |
| CN104441182A (en) * | 2014-12-12 | 2015-03-25 | 如皋市东陈镇爱红工艺品厂 | Novel antibacterial anti-mildew bamboo carving handicraft manufacturing method |
| CN105685105A (en) * | 2016-03-25 | 2016-06-22 | 福建师范大学 | Method for preparing high-protein bacterial powder by utilizing antibiotic fungus residues |
| CN106434439A (en) * | 2016-09-21 | 2017-02-22 | 云南农业大学 | Lysobacter enzymogenes 1-T-1-4 and application thereof |
| CN106493826A (en) * | 2016-10-07 | 2017-03-15 | 常州创索新材料科技有限公司 | A kind of preparation method of anti-bacterial fibre plate |
| CN108356944A (en) * | 2018-02-10 | 2018-08-03 | 张芸 | A kind of preparation method of wood-based plate mould inhibitor |
| CN109049251A (en) * | 2018-08-22 | 2018-12-21 | 浙江恋家生活家居有限公司 | A kind of processing technology of deformation-preventing wooden door |
| CN110436456A (en) * | 2019-07-08 | 2019-11-12 | 黄山市黟县宏联竹制工艺有限公司 | A kind of production technology of shuttle shape environmental protection bamboo stick |
| CN111671777A (en) * | 2020-06-30 | 2020-09-18 | 福州科力恩生物科技有限公司 | Microbial disinfectant, and preparation method and application thereof |
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