CN105459243B - A kind of processing method with white rot bacterial plaque China fir Furniture panel - Google Patents

A kind of processing method with white rot bacterial plaque China fir Furniture panel Download PDF

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CN105459243B
CN105459243B CN201610007430.5A CN201610007430A CN105459243B CN 105459243 B CN105459243 B CN 105459243B CN 201610007430 A CN201610007430 A CN 201610007430A CN 105459243 B CN105459243 B CN 105459243B
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china fir
whiterot fungi
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CN105459243A (en
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黄晓东
黄俊昆
刘雁
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Shenxian Lukai Comprehensive Service Co ltd
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Fujian Agriculture and Forestry University
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B27WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27MWORKING OF WOOD NOT PROVIDED FOR IN SUBCLASSES B27B - B27L; MANUFACTURE OF SPECIFIC WOODEN ARTICLES
    • B27M3/00Manufacture or reconditioning of specific semi-finished or finished articles
    • B27M3/18Manufacture or reconditioning of specific semi-finished or finished articles of furniture or of doors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B27WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27KPROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

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Abstract

A kind of processing method with white rot bacterial plaque China fir Furniture panel.The China fir ecological sheet material of outsourcing is sterilized using high-temperature steam first and humidification process, kill other miscellaneous bacterias that outsourcing fir wood plank may carry, prevent occurring the phenomenon that miscellaneous bacteria infects during whiterot fungi cultivate;Secondly, white rot bacteria culture fluid is prepared, is finally made whiterot fungi bacterium solution;Recycle sprayer that whiterot fungi bacterium solution is sprayed to the China fir ecological sheet material surface of brushing culture medium, be sent into the culture of progress whiterot fungi bacterial plaque between constant temperature and humidity;Finally, the China fir ecological sheet material for surface being covered to whiterot fungi bacterial plaque is sent into wood dryer drying, and the Furniture panel material for having unique decorative effect is made.This process technology is simple and easy, using cheap fir wood plank, handled through special process, produce unique China fir Furniture panel with white rot bacterial plaque, this technology disclosure satisfy that fitment material for family modelled after an antique makees old needs in furniture and house decoration, is substantially improved the economic value of fir wood plank.

Description

A kind of processing method with white rot bacterial plaque China fir Furniture panel
Technical field
The invention belongs to wood processing field, and in particular to a kind of processing method with white rot bacterial plaque China fir Furniture panel.
Background technology
It is a kind of feelings to miss old times or old friends, and when the life of people more tends to steady, more cherishes the memory of those time passed.People often touch Scape life feelings wake up some fine memories, and nature, the feelings of back to nature are yearned in release.As popular several years ago jeans event As meaning does old worn-off, nowadays furniture is also swept " doing old wind ".Simple and unsophisticated furniture, elegant moulding, one advocates ancient customs Gu rhythm " doing old " wind it is popular.Timeworn furniture meet modern trend, disappeared by white collar and middle production because the time is remote with more vicissitudes sense Expense person pursues, expensive although furniture surface is outmoded.
The furniture of American-European countries especially praises highly the pseudo-classic style missed old times or old friends.As the development of China's furniture industry, outlet are American-European Furniture account for furniture total amount most.Meet American-European customer demand antique imitation process have become furniture surface processing and The prevailing technology of application, this antique-imitation technique are also connect with the reinforcement of international exchange by domestic a part of consumer group By, while in the classic furniture of Chinese Style, with the covering with paint of antique traditional furniture, antique-imitation technique also has certain applications.
The cheap fir wood plank of this processing and utilization, is handled through special process, produces unique China fir with white rot bacterial plaque Wooden furniture sheet material, this technology disclosure satisfy that fitment material for family modelled after an antique makees old needs in furniture and house decoration, is substantially improved China fir The economic value of wood plank, therefore there is vast potential for future development.
The content of the invention
It is an object of the invention to provide a kind of processing method with white rot bacterial plaque China fir Furniture panel.This method exploitation value The cheap fir wood plank of lattice, is handled through special process, produces unique China fir Furniture panel with white rot bacterial plaque, this technology energy Enough meet that fitment material for family modelled after an antique makees old needs in furniture and house decoration, is substantially improved the economic value of fir wood plank.
The invention provides a kind of processing method with white rot bacterial plaque China fir Furniture panel, this method comprises the following steps:
(1)Outsourcing specification China fir ecological sheet material(2440mm×1220mm×18mm)(length × width x thickness), it is sent into timber and does Dry kiln carries out high-temp steam sterilizing and humidification process, standby;
(2)Take 24 g potato dextrose culture-mediums(PDb)Load 2000 milliliters of glass with 15~20 grams of agar (Agar) In bottle, 1000 milliliters of distilled water are added, after stirring, is put into high-temperature sterilization box and carries out sterilization treatment;Taken after 1~2 hour Go out, pour into blank, after cooling down 5~15 minutes, after nutrient solution is in solid-like, covers cover plate and be put into incubator, it is standby;
(3)The situation of solid medium in incubator blank is checked daily, and as found, the culture medium in blank has miscellaneous bacteria sense Dye, picks discarding immediately;
(4)Whiterot fungi strain is provided by the Chinese Academy of Forestry's life ring, using connecing acicula respectively to the culture in blank Base connects bacterium, and blank is put into incubator by completion after connecing bacterium work, takes out the growing state of observation whiterot fungi daily, and discovery has miscellaneous The blank of bacterium infection must pick discarding;
(5)After the mould spot area of whiterot fungi develops into account for whole blank culture medium area 60~80%, take out white Rotten bacterium blank, pour into the crushing of stainless steel pulverizer.By whiterot fungi:Distilled water=1:100(Mass ratio)Ratio add distilled water, Whiterot fungi bacterium solution is modulated, is fitted into after mixing well in the reagent bottle of manual pesticide sprayer, it is standby;
(6)By potato dextrose medium:Agar:Distilled water=24:18:1000 (mass ratioes) prepare nutrient solution, load It is sent into after container in high-temperature sterilization box and carries out sterilization treatment, is taken out after 1.5 hours, equably brushed by high temperature with hairbrush On steam sterilizing and the China fir ecological board of humidification process;
(7)The China fir ecological sheet material for completing step 6 is placed on table top, by China fir ecological sheet material area(m2):White rot Bacterium bacterium solution(g)=1:Whiterot fungi bacterium solution is uniformly sprayed on China fir ecological sheet material by 1~2 ratio with manual pesticide sprayer, complete By between China fir ecological sheet material feeding constant temperature and humidity after into sprinkling, lie in a horizontal plane in homemade metal layer frame(Sprinkling is face-up), The each interval of metal layer frame is 8cm, and every layer is placed a China fir ecological sheet material, height of the metal layer frame extreme lower position away from ground 10-15cm;
(8)The situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, such as finds China fir ecological sheet material Surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
(9)China fir ecological sheet material surface to be found whiterot fungi growth area exceed China fir ecological board surface area 80% with After upper, China fir ecological sheet material is taken out between constant temperature and humidity, lumber kiln is sent into and is dried processing, drying temperature 80~ 100 DEG C, band white rot bacterial plaque China fir furniture-plates are made 8~10% in 24~48 hours drying times, dried moisture control Material.
Outsourcing specification China fir ecological sheet material is sent into the mesh that lumber kiln carries out high-temp steam sterilizing processing in the step 1 Be the thorough other miscellaneous bacterias killed outsourcing fir wood plank and may carried, prevent miscellaneous bacteria sense occur during whiterot fungi is cultivated The phenomenon of dye.At 120 DEG C, processing time controlled at 20~30 minutes the temperature control of steam high-temperature sterilization.
The purpose that outsourcing specification China fir ecological sheet material is sent into lumber kiln progress humidification process in the step 1 is will be outer The moisture content of purchase China fir ecological sheet material brings up to 30~60% from 10%, is advantageous to the normal growth and development of later stage whiterot fungi.
After the preparation that nutrient solution is completed in the step 2, blank must be sent into incubator the culture carried out 3 days by a definite date Journey, at 25 ± 2 DEG C, relative humidity controls 85~90% the temperature control of incubator.
In the step 4 using connect acicula respectively give blank in culture medium inoculated whiterot fungi process, it is miscellaneous in order to prevent The infection of bacterium, whole seeded process must be completed in sterile operating room, it is necessary to open the exhaust fan ventilation of fume hood, it is necessary to 1% Alcohol liquid to workbench pasteurised completely, during bacterium is connect, connecing acicula must use alcolhol burner calcination to sterilize.
Whiterot fungi growing state is observed in the step 4 daily, in order to prevent the infection of miscellaneous bacteria, blank observation must not be opened Whiterot fungi growing state.
During whiterot fungi bacterium solution is prepared in the step 5, the stainless steel pulverizer crushed for whiterot fungi is using Before have to pass through alcohol disinfecting, prepare the distilled water used in whiterot fungi bacterium solution and must assure that definitely without mycotic infection, for spraying The pesticide sprayer of whiterot fungi bacterium solution is also having to pass through alcohol disinfecting using preceding.
In the step 6 during preparation and brushing nutrient solution, the dosage of nutrient solution, which must assure that, to be used while allocating, and is prevented Only brushed caused by preparing excessive nutrient solution not as good as gel is formed, produce unnecessary loss, fir wood plank one side nutrient solution applies The control of brush amount exists:260~300g/m2
The China fir ecological sheet material that one side sprinkling whiterot fungi bacterium solution is completed in the step 7 is sent between constant temperature and humidity, and constant temperature is permanent At 25 ± 2 DEG C, relative humidity controls 85~90% the temperature control of wet;Place the metal frame extreme lower position of China fir ecological board Height away from ground is more than 10cm, prevents other miscellaneous bacterias on ground from infecting.
Find that the whiterot fungi growth area on China fir ecological sheet material surface exceedes China fir ecological board surface area in the step 9 After more than 80%, processing is dried in China fir ecological board feeding lumber kiln, is formed for protection China fir ecology plate surface white The neat and integrality of rotten bacterial plaque, notice that China fir ecological board must individually be done in independent metallic spacer layer in drying process It is dry.
The advantage of the invention is that:A kind of processing method with white rot bacterial plaque China fir Furniture panel is provided.This method utilizes Cheap fir wood plank, is handled through special process, produces unique China fir Furniture panel with white rot bacterial plaque, this technology It disclosure satisfy that fitment material for family modelled after an antique makees old needs in furniture and house decoration, is substantially improved the economic value of fir wood plank.
Embodiment
Embodiment 1
A kind of processing method with white rot bacterial plaque China fir Furniture panel concretely comprises the following steps:
Step 1:Outsourcing specification China fir ecological sheet material(2440mm×1220mm×18mm)(length × width x thickness), it is sent into timber Dry kiln carries out high-temp steam sterilizing and humidification process, standby;
Step 2:Take 24 g potato dextrose culture-mediums(PDb)Load 2000 milliliters of vials with 15 grams of agar (Agar) In, 1000 milliliters of distilled water are added, after stirring, is put into high-temperature sterilization box and carries out sterilization treatment;Take out, fall after 1 hour Enter in blank, after cooling down 5 minutes, after nutrient solution is in solid-like, covers cover plate and be put into incubator, it is standby;
Step 3:The situation of solid medium in incubator blank is checked daily, and as found, the culture medium in blank has miscellaneous bacteria Infection, picks discarding immediately;
Step 4:Whiterot fungi strain is provided by the Chinese Academy of Forestry's life ring, using connecing acicula respectively to the training in blank Foster base connects bacterium, and blank is put into incubator, takes out the growing state of observation whiterot fungi daily, found by completion after connecing bacterium work The blank of miscellaneous bacteria infection must pick discarding;
Step 5:After the mould spot area of whiterot fungi develops into account for whole blank culture medium area 60%, take out white Rotten bacterium blank, pour into the crushing of stainless steel pulverizer.By whiterot fungi:Distilled water=1:100(Mass ratio)Ratio add distilled water, Whiterot fungi bacterium solution is modulated, is fitted into after mixing well in the reagent bottle of manual pesticide sprayer, it is standby;
Step 6:By potato dextrose medium:Agar:Distilled water=24:18:1000 (mass ratioes) prepare nutrient solution, It is fitted into be sent into high-temperature sterilization box after container and carries out sterilization treatment, is taken out after 1.5 hours, equably brushed and passed through with hairbrush On high-temp steam sterilizing and the China fir ecological board of humidification process;
Step 7:The China fir ecological sheet material for completing step 6 is placed on table top, by China fir ecological sheet material area(m2):In vain Rotten bacterium bacterium solution(g)=1:Whiterot fungi bacterium solution is uniformly sprayed on China fir ecological sheet material by 1 ratio with manual pesticide sprayer, complete By between China fir ecological sheet material feeding constant temperature and humidity after into sprinkling, lie in a horizontal plane in homemade metal layer frame(Sprinkling is face-up), The each interval of metal layer frame is 8cm, and every layer is placed a China fir ecological sheet material, height of the metal layer frame extreme lower position away from ground 10cm;
Step 8:The situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, such as finds China fir ecology Plate surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
Step 9:The whiterot fungi growth area on China fir ecological sheet material surface to be found exceedes the 80% of China fir ecological board surface area After above, China fir ecological sheet material is taken out between constant temperature and humidity, lumber kiln is sent into and processing, drying temperature 80 is dried DEG C, band white rot bacterial plaque China fir Furniture panel is made 8% in 48 hours drying times, dried moisture control.
Outsourcing specification China fir ecological sheet material is sent into the mesh that lumber kiln carries out high-temp steam sterilizing processing in the step 1 Be the thorough other miscellaneous bacterias killed outsourcing fir wood plank and may carried, prevent miscellaneous bacteria sense occur during whiterot fungi is cultivated The phenomenon of dye.At 120 DEG C, processing time controlled at 20 minutes the temperature control of steam high-temperature sterilization.
The purpose that outsourcing specification China fir ecological sheet material is sent into lumber kiln progress humidification process in the step 1 is will be outer The moisture content of purchase China fir ecological sheet material brings up to 30~60% from 10%, is advantageous to the normal growth and development of later stage whiterot fungi.
After the preparation that nutrient solution is completed in the step 2, blank must be sent into incubator the culture carried out 3 days by a definite date Journey, at 25 ± 2 DEG C, relative humidity controls 85% the temperature control of incubator.
In the step 4 using connect acicula respectively give blank in culture medium inoculated whiterot fungi process, it is miscellaneous in order to prevent The infection of bacterium, whole seeded process must be completed in sterile operating room, it is necessary to open the exhaust fan ventilation of fume hood, it is necessary to 1% Alcohol liquid to workbench pasteurised completely, during bacterium is connect, connecing acicula must use alcolhol burner calcination to sterilize.
Whiterot fungi growing state is observed in the step 4 daily, in order to prevent the infection of miscellaneous bacteria, blank observation must not be opened Whiterot fungi growing state.
During whiterot fungi bacterium solution is prepared in the step 5, the stainless steel pulverizer crushed for whiterot fungi is using Before have to pass through alcohol disinfecting, prepare the distilled water used in whiterot fungi bacterium solution and must assure that definitely without mycotic infection, for spraying The pesticide sprayer of whiterot fungi bacterium solution is also having to pass through alcohol disinfecting using preceding.
In the step 6 during preparation and brushing nutrient solution, the dosage of nutrient solution, which must assure that, to be used while allocating, and is prevented Only brushed caused by preparing excessive nutrient solution not as good as gel is formed, produce unnecessary loss, fir wood plank one side nutrient solution applies The control of brush amount exists:260g/m2
The China fir ecological sheet material that one side sprinkling whiterot fungi bacterium solution is completed in the step 7 is sent between constant temperature and humidity, and constant temperature is permanent At 25 ± 2 DEG C, relative humidity controls 85% the temperature control of wet;The metal frame extreme lower position of China fir ecological board is placed away from ground The height 10cm in face, prevent other miscellaneous bacterias on ground from infecting.
Find that the whiterot fungi growth area on China fir ecological sheet material surface exceedes China fir ecological board surface area in the step 9 After more than 80%, processing is dried in China fir ecological board feeding lumber kiln, is formed for protection China fir ecology plate surface white The neat and integrality of rotten bacterial plaque, notice that China fir ecological board must individually be done in independent metallic spacer layer in drying process It is dry.
Embodiment 2
A kind of processing method with white rot bacterial plaque China fir Furniture panel concretely comprises the following steps:
Step 1:Outsourcing specification China fir ecological sheet material(2440mm×1220mm×18mm)(length × width x thickness), it is sent into timber Dry kiln carries out high-temp steam sterilizing and humidification process, standby;
Step 2:Take 24 g potato dextrose culture-mediums(PDb)Load 2000 milliliters of vials with 20 grams of agar (Agar) In, 1000 milliliters of distilled water are added, after stirring, is put into high-temperature sterilization box and carries out sterilization treatment;Take out, fall after 2 hours Enter in blank, after cooling down 15 minutes, after nutrient solution is in solid-like, covers cover plate and be put into incubator, it is standby;
Step 3:The situation of solid medium in incubator blank is checked daily, and as found, the culture medium in blank has miscellaneous bacteria Infection, picks discarding immediately;
Step 4:Whiterot fungi strain is provided by the Chinese Academy of Forestry's life ring, using connecing acicula respectively to the training in blank Foster base connects bacterium, and blank is put into incubator, takes out the growing state of observation whiterot fungi daily, found by completion after connecing bacterium work The blank of miscellaneous bacteria infection must pick discarding;
Step 5:After the mould spot area of whiterot fungi develops into account for whole blank culture medium area 80%, take out white Rotten bacterium blank, pour into the crushing of stainless steel pulverizer.By whiterot fungi:Distilled water=1:100(Mass ratio)Ratio add distilled water, Whiterot fungi bacterium solution is modulated, is fitted into after mixing well in the reagent bottle of manual pesticide sprayer, it is standby;
Step 6:By potato dextrose medium:Agar:Distilled water=24:18:1000 (mass ratioes) prepare nutrient solution, It is fitted into be sent into high-temperature sterilization box after container and carries out sterilization treatment, is taken out after 1.5 hours, equably brushed and passed through with hairbrush On high-temp steam sterilizing and the China fir ecological board of humidification process;
Step 7:The China fir ecological sheet material for completing step 6 is placed on table top, by China fir ecological sheet material area(m2):In vain Rotten bacterium bacterium solution(g)=1:Whiterot fungi bacterium solution is uniformly sprayed on China fir ecological sheet material by 2 ratio with manual pesticide sprayer, complete By between China fir ecological sheet material feeding constant temperature and humidity after into sprinkling, lie in a horizontal plane in homemade metal layer frame(Sprinkling is face-up), The each interval of metal layer frame is 8cm, and every layer is placed a China fir ecological sheet material, height of the metal layer frame extreme lower position away from ground 15cm;
Step 8:The situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, such as finds China fir ecology Plate surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
Step 9:The whiterot fungi growth area on China fir ecological sheet material surface to be found exceedes the 80% of China fir ecological board surface area After above, China fir ecological sheet material is taken out between constant temperature and humidity, lumber kiln is sent into and processing, drying temperature 100 is dried DEG C, band white rot bacterial plaque China fir Furniture panel is made 10% in 24 hours drying times, dried moisture control.
Preferably, outsourcing specification China fir ecological sheet material is sent into lumber kiln and carries out high-temp steam sterilizing in the step 1 The purpose of processing is the thorough other miscellaneous bacterias killed outsourcing fir wood plank and may carried, and prevents from going out during whiterot fungi is cultivated The phenomenon of existing miscellaneous bacteria infection.At 120 DEG C, processing time controlled at 30 minutes the temperature control of steam high-temperature sterilization.
Preferably, outsourcing specification China fir ecological sheet material is sent into the mesh that lumber kiln carries out humidification process in the step 1 Be that the moisture content of outsourcing China fir ecological sheet material is brought up to 60% from 10%, be advantageous to the normal growth and development of later stage whiterot fungi.
Preferably, after the preparation that nutrient solution is completed in the step 2, blank, which must be sent into incubator, to be carried out 3 days by a definite date Incubation, at 25 ± 2 DEG C, relative humidity is controlled 90% for the temperature control of incubator.
Preferably, in the step 4 using connect acicula respectively give blank in culture medium inoculated whiterot fungi process, in order to The infection of miscellaneous bacteria is prevented, whole seeded process must be completed in sterile operating room, it is necessary to the exhaust fan ventilation of fume hood is opened, must Must be with 1% alcohol liquid to workbench pasteurised completely, during bacterium is connect, connecing acicula must use alcolhol burner calcination to sterilize.
Preferably, whiterot fungi growing state is observed in the step 4 daily, in order to prevent the infection of miscellaneous bacteria, must not be opened Blank observes whiterot fungi growing state.
Preferably, during preparing whiterot fungi bacterium solution in the step 5, the stainless steel pulverizer for whiterot fungi crushing It must assure that definitely without mycotic infection, use using alcohol disinfecting is preceding had to pass through, preparing the distilled water used in whiterot fungi bacterium solution In the pesticide sprayer of sprinkling whiterot fungi bacterium solution alcohol disinfecting is also being had to pass through using preceding.
Preferably, in the step 6 during preparation and brushing nutrient solution, the dosage of nutrient solution must assure that with With with preventing from brushing caused by preparing excessive nutrient solution not as good as gel is formed, produce unnecessary loss, the training of fir wood plank one side The control of nutrient solution brushing amount exists: 300g/m2
Preferably, the China fir ecological sheet material that one side sprinkling whiterot fungi bacterium solution is completed in the step 7 is sent between constant temperature and humidity, At 25 ± 2 DEG C, relative humidity controls 90% temperature control between constant temperature and humidity;Place the metal frame lowest order of China fir ecological board The height 15cm away from ground is put, prevents other miscellaneous bacterias on ground from infecting.
Preferably, find that the whiterot fungi growth area on China fir ecological sheet material surface exceedes China fir ecological board in the step 9 After more than the 80% of surface area, processing is dried in China fir ecological board feeding lumber kiln, to protect China fir ecology plate surface The neat and integrality of the white rot bacterial plaque of formation, notice that China fir ecological board must be mounted in independent metallic spacer layer in drying process Individually dry.
Embodiment 3
A kind of processing method with white rot bacterial plaque China fir Furniture panel concretely comprises the following steps:
Step 1:Outsourcing specification China fir ecological sheet material(2440mm×1220mm×18mm)(length × width x thickness), it is sent into timber Dry kiln carries out high-temp steam sterilizing and humidification process, standby;
Step 2:Take 24 g potato dextrose culture-mediums(PDb)Load 2000 milliliters of vials with 17 grams of agar (Agar) In, 1000 milliliters of distilled water are added, after stirring, is put into high-temperature sterilization box and carries out sterilization treatment;Taken out after 1.5 hours, Pour into blank, after cooling down 10 minutes, after nutrient solution is in solid-like, covers cover plate and be put into incubator, it is standby;
Step 3:The situation of solid medium in incubator blank is checked daily, and as found, the culture medium in blank has miscellaneous bacteria Infection, picks discarding immediately;
Step 4:Whiterot fungi strain is provided by the Chinese Academy of Forestry's life ring, using connecing acicula respectively to the training in blank Foster base connects bacterium, and blank is put into incubator, takes out the growing state of observation whiterot fungi daily, found by completion after connecing bacterium work The blank of miscellaneous bacteria infection must pick discarding;
Step 5:After the mould spot area of whiterot fungi develops into account for whole blank culture medium area 80%, take out white Rotten bacterium blank, pour into the crushing of stainless steel pulverizer.By whiterot fungi:Distilled water=1:100(Mass ratio)Ratio add distilled water, Whiterot fungi bacterium solution is modulated, is fitted into after mixing well in the reagent bottle of manual pesticide sprayer, it is standby;
Step 6:By potato dextrose medium:Agar:Distilled water=24:18:1000 (mass ratioes) prepare nutrient solution, It is fitted into be sent into high-temperature sterilization box after container and carries out sterilization treatment, is taken out after 1.5 hours, equably brushed and passed through with hairbrush On high-temp steam sterilizing and the China fir ecological board of humidification process;
Step 7:The China fir ecological sheet material for completing step 6 is placed on table top, by China fir ecological sheet material area(m2):In vain Rotten bacterium bacterium solution(g)=1:Whiterot fungi bacterium solution is uniformly sprayed on China fir ecological sheet material by 2 ratio with manual pesticide sprayer, complete By between China fir ecological sheet material feeding constant temperature and humidity after into sprinkling, lie in a horizontal plane in homemade metal layer frame(Sprinkling is face-up), The each interval of metal layer frame is 8cm, and every layer is placed a China fir ecological sheet material, height of the metal layer frame extreme lower position away from ground 10cm;
Step 8:The situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, such as finds China fir ecology Plate surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
Step 9:The whiterot fungi growth area on China fir ecological sheet material surface to be found exceedes the 90% of China fir ecological board surface area After above, China fir ecological sheet material is taken out between constant temperature and humidity, lumber kiln is sent into and processing, drying temperature 90 is dried DEG C, band white rot bacterial plaque China fir Furniture panel is made 9% in 36 hours drying times, dried moisture control.
Preferably, outsourcing specification China fir ecological sheet material is sent into lumber kiln and carries out high-temp steam sterilizing in the step 1 The purpose of processing is the thorough other miscellaneous bacterias killed outsourcing fir wood plank and may carried, and prevents from going out during whiterot fungi is cultivated The phenomenon of existing miscellaneous bacteria infection.At 120 DEG C, processing time controlled at 25 minutes the temperature control of steam high-temperature sterilization.
Preferably, outsourcing specification China fir ecological sheet material is sent into the mesh that lumber kiln carries out humidification process in the step 1 Be that the moisture content of outsourcing China fir ecological sheet material is brought up to 50% from 10%, be advantageous to the normal growth and development of later stage whiterot fungi.
Preferably, after the preparation that nutrient solution is completed in the step 2, blank, which must be sent into incubator, to be carried out 3 days by a definite date Incubation, at 25 ± 2 DEG C, relative humidity is controlled 90% for the temperature control of incubator.
Preferably, in the step 4 using connect acicula respectively give blank in culture medium inoculated whiterot fungi process, in order to The infection of miscellaneous bacteria is prevented, whole seeded process must be completed in sterile operating room, it is necessary to the exhaust fan ventilation of fume hood is opened, must Must be with 1% alcohol liquid to workbench pasteurised completely, during bacterium is connect, connecing acicula must use alcolhol burner calcination to sterilize.
Preferably, whiterot fungi growing state is observed in the step 4 daily, in order to prevent the infection of miscellaneous bacteria, must not be opened Blank observes whiterot fungi growing state.
Preferably, during preparing whiterot fungi bacterium solution in the step 5, the stainless steel pulverizer for whiterot fungi crushing It must assure that definitely without mycotic infection, use using alcohol disinfecting is preceding had to pass through, preparing the distilled water used in whiterot fungi bacterium solution In the pesticide sprayer of sprinkling whiterot fungi bacterium solution alcohol disinfecting is also being had to pass through using preceding.
Preferably, in the step 6 during preparation and brushing nutrient solution, the dosage of nutrient solution must assure that with With with preventing from brushing caused by preparing excessive nutrient solution not as good as gel is formed, produce unnecessary loss, the training of fir wood plank one side The control of nutrient solution brushing amount exists: 300g/m2
Preferably, the China fir ecological sheet material that one side sprinkling whiterot fungi bacterium solution is completed in the step 7 is sent between constant temperature and humidity, At 25 ± 2 DEG C, relative humidity controls 90% temperature control between constant temperature and humidity;Place the metal frame lowest order of China fir ecological board The height 10cm away from ground is put, prevents other miscellaneous bacterias on ground from infecting.
Preferably, find that the whiterot fungi growth area on China fir ecological sheet material surface exceedes China fir ecological board in the step 9 After more than the 80% of surface area, processing is dried in China fir ecological board feeding lumber kiln, to protect China fir ecology plate surface The neat and integrality of the white rot bacterial plaque of formation, notice that China fir ecological board must be mounted in independent metallic spacer layer in drying process Individually dry.
The band white rot bacterial plaque China fir Furniture panel prepared by the present invention, there is the characteristics of simple to operate, easy to learn, this method Cheap fir wood plank can be utilized, is handled through special process, produces band white rot bacterial plaque China fir Furniture panel, this skill Art disclosure satisfy that fitment material for family modelled after an antique makees old needs in furniture and house decoration, is substantially improved the economic value of fir wood plank, Therefore there is vast potential for future development.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, it should all belong to the covering scope of the present invention.

Claims (1)

1. a kind of processing method with white rot bacterial plaque China fir Furniture panel, it is characterised in that comprise the following steps:
(1)Outsourcing specification China fir ecological sheet material, length × width x thickness=2440mm × 1220mm × 18mm, it is sent into lumber kiln and enters Row high-temp steam sterilizing and humidification process, it is standby;
(2)Take 24 g potato dextrose culture-mediums and 15~20 grams of agar to be fitted into 2000 milliliters of vials, add 1000 millis Distilled water is risen, after stirring, is put into high-temperature sterilization box and carries out sterilization treatment;Take out, poured into blank after 1~2 hour, After cooling 5~15 minutes, after nutrient solution is in solid-like, covers cover plate and be put into incubator, it is standby;
(3)The situation of solid medium in incubator blank is checked daily, and as found, the culture medium in blank has miscellaneous bacteria infection, stands Pick discarding;
(4)Bacterium is connect to the culture medium in blank respectively using acicula is connect, for the bacterium used for whiterot fungi, completion will be white after connecing bacterium work Plate is put into incubator, takes out the growing state of observation whiterot fungi daily, and the blank for finding to have miscellaneous bacteria to infect must pick discarding;
(5)After the mould spot area of whiterot fungi develops into and accounts for the 60~80% of whole blank culture medium area, it is white to take out whiterot fungi Plate, the crushing of stainless steel pulverizer is poured into, by whiterot fungi:Distilled water mass ratio=1:100 ratio adds distilled water, modulates white rot Bacterium bacterium solution, it is fitted into after mixing well in the reagent bottle of manual pesticide sprayer, it is standby;
(6)By potato dextrose medium:Agar:Distilled water mass ratio=24:18:1000 prepare nutrient solution, after loading container It is sent into high-temperature sterilization box and carries out sterilization treatment, takes out after 1.5 hours, equably brushed by high-temp steam sterilizing with hairbrush And on the China fir ecological board of humidification process;
(7)The China fir ecological sheet material for completing step 6 is placed on table top, by China fir ecological sheet material area:Whiterot fungi bacterium solution matter Amount=1:Whiterot fungi bacterium solution is uniformly sprayed on China fir ecological sheet material by 1~2 ratio with manual pesticide sprayer, completes sprinkling Afterwards by between China fir ecological sheet material feeding constant temperature and humidity, lie in a horizontal plane in homemade metal layer frame, sprinkling is face-up, metal layer frame Each interval be 8cm, one China fir ecological sheet material of every layer of placement, height 10-15cm of the metal layer frame extreme lower position away from ground;
(8)The situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, such as finds China fir ecological sheet material surface Moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
(9)After the whiterot fungi growth area on China fir ecological sheet material surface to be found exceedes more than the 80% of China fir ecological board surface area, China fir ecological sheet material is taken out between constant temperature and humidity, lumber kiln is sent into and is dried processing, 80~100 DEG C of drying temperature, Band white rot bacterial plaque China fir Furniture panel is made 8~10% in 24~48 hours drying times, dried moisture control;
At 120 DEG C, processing time controlled at 20~30 minutes the temperature control of step 1 high temperature steam sterilizing;
The purpose that outsourcing specification China fir ecological sheet material is sent into that lumber kiln carries out humidification process in step 1 is to give birth to outsourcing China fir The moisture content of state sheet material brings up to 30~60% from 10%, is advantageous to the normal growth and development of later stage whiterot fungi;
After the preparation that nutrient solution is completed in step 2, blank must be sent into incubator the incubation carried out 3 days by a definite date, culture At 25 ± 2 DEG C, relative humidity controls 85~90% the temperature control of case;
Using process of the acicula respectively to the culture medium inoculated whiterot fungi in blank is connect in step 4, in order to prevent the infection of miscellaneous bacteria, Whole seeded process must be completed in sterile operating room, it is necessary to open the exhaust fan ventilation of fume hood, it is necessary to 1% alcohol liquid To workbench pasteurised completely, during bacterium is connect, connecing acicula must use alcolhol burner calcination to sterilize;
Whiterot fungi growing state is observed in step 4 daily, in order to prevent the infection of miscellaneous bacteria, blank observation whiterot fungi life must not be opened Long situation;
During whiterot fungi bacterium solution is prepared in step 5, the stainless steel pulverizer crushed for whiterot fungi must be through before use Alcohol disinfecting is crossed, the distilled water used in whiterot fungi bacterium solution is prepared and must assure that definitely without mycotic infection, for spraying whiterot fungi bacterium The pesticide sprayer of liquid is also having to pass through alcohol disinfecting using preceding;
In step 6 during preparation and brushing nutrient solution, the dosage of nutrient solution, which must assure that, to be used while allocating, and is prevented because preparing Excessive nutrient solution causes to brush not as good as gel is formed, and produces unnecessary loss, the control of fir wood plank one side nutrient solution brushing amount :260~300g/m2
The China fir ecological sheet material that one side sprinkling whiterot fungi bacterium solution is completed in step 7 is sent between constant temperature and humidity, the temperature between constant temperature and humidity At 25 ± 2 DEG C, relative humidity is controlled 85~90% for degree control;The metal frame extreme lower position of China fir ecological board is placed away from ground Height 10-15cm, prevent other miscellaneous bacterias on ground from infecting;
Find that the whiterot fungi growth area on China fir ecological sheet material surface exceedes more than the 80% of China fir ecological board surface area in step 9 Afterwards, processing is dried in China fir ecological board feeding lumber kiln, the white rot bacterial plaque formed for protection China fir ecology plate surface Neat and integrality, notice in drying process that China fir ecological board must be mounted in independent metallic spacer layer and individually dry.
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CN106003345B (en) * 2016-06-01 2017-11-21 福建农林大学 Old processing method is made in a kind of Chinese wax timber floor sootiness
CN107457880A (en) * 2017-09-15 2017-12-12 淮阴师范学院 Utilize the method for white rot mycelium production non-glue fibre board
CN109015995A (en) * 2018-07-23 2018-12-18 南通市紫石古典园林建筑工程有限公司 A kind of building timber antique-imitation processing method

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CN103497897B (en) * 2013-04-19 2016-02-03 西南林业大学 One strain Phomopsis fungi and the application in preparation bacterium line wood thereof
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CN103525707B (en) * 2013-04-19 2017-04-12 西南林业大学 Nectria rigidiuscula and application thereof in preparation of bacterium grain wood
CN103756910B (en) * 2013-04-19 2017-04-12 西南林业大学 Xylaria venosula fungi and application of the same in preparation of fungi pattern wood
CN103963122B (en) * 2014-05-09 2016-03-16 阜南县方柳工艺品有限公司 A kind of preparation method of wooden boat modelled after an antique
CN104070571B (en) * 2014-06-17 2016-04-06 福建农林大学 A kind ofly utilize Aspergillus flavus to the method preventing bamboo wood from going mouldy
CN104015238B (en) * 2014-06-23 2015-11-18 福建农林大学 A kind of mildew-proof treatment method again contaminating mould bamboo chip
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