CN111939211A

CN111939211A - Traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronary infection and preparation method thereof

Info

Publication number: CN111939211A
Application number: CN202010890415.6A
Authority: CN
Inventors: 吴正治; 梁少瑜; 曾永长; 贾丹; 李子雯; 汪梦霞; 刘洁人
Original assignee: Shenzhen Institute of Gerontology
Current assignee: Shenzhen Institute of Gerontology
Priority date: 2020-07-17
Filing date: 2020-08-29
Publication date: 2020-11-17

Abstract

The invention provides a traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronal infection, which is prepared from the following raw materials in parts by weight: 1-30 parts of kudzu root, 1-10 parts of cape jasmine fruit, 1-15 parts of honeysuckle flower, 2-30 parts of houttuynia cordata, 1-20 parts of sea buckthorn leaf, 1-15 parts of perillaseed, 1-12 parts of almond, 1-10 parts of peach kernel, 1-10 parts of platycodon grandiflorum and 1-10 parts of green tea. And a preparation method of the traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronary infection is provided. The research result of the formula component-target point-pharmacological action mechanism network of the traditional Chinese medicine composition for the auxiliary treatment of the middle stage of new coronary infection is consistent with the linkage of the main treatment symptoms, and the modern precise medical principle is met. Meanwhile, the product has low cost and meets the requirement of industrialized production.

Description

Traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronary infection and preparation method thereof

Technical Field

The invention belongs to the technical field of health care products, and particularly relates to a traditional Chinese medicine composition for adjuvant therapy of neocoronal infection in the middle stage and a preparation method thereof.

Background

The traditional Chinese medicine is considered to have definite curative effect on the new coronary pneumonia, and besides clinical formulas, the Chinese patent medicines are also recommended to be used for preventing and treating the new coronary pneumonia, such as wrinkled gianthyssop vital essence water, honeysuckle flower antipyretic capsules, golden flower cold-clearing granules, jade screen powder, double coptis, ephedra, gypsum and licorice decoction, honeysuckle flower and forsythia powder, kudzu-vine root and scutellaria and the like, but the medicines belong to medicine products, and the medical and edible homologous products have wide application prospects in the aspects of early prevention, medium treatment and later recovery of the new coronary pneumonia, and no related products exist in the market at present. Particularly aiming at the new coronary pneumonia virus infection, no related medicinal and edible health products except the traditional Chinese medicine prescription exist in the treatment period.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a traditional Chinese medicine composition for adjuvant therapy of new crown infection in the middle stage and a preparation method thereof.

The invention provides a traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronal infection, which is prepared from the following raw materials in parts by weight:

1 to 30 parts of kudzu-vine root,

1 to 10 parts of gardenia, and the like,

1-15 parts of honeysuckle flower,

2-30 parts of houttuynia cordata,

1-20 parts of sea buckthorn leaves,

1-15 parts of perillaseed,

1-12 parts of almond, namely almond,

1 to 10 parts of peach kernel,

1-10 parts of platycodon grandiflorum,

1-10 parts of green tea.

And a preparation method of the traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronal infection, which comprises the following steps:

weighing the raw materials according to the weight parts of the traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronal infection, mixing kudzu vine root, cape jasmine fruit, honeysuckle flower, houttuynia cordata, sea backthern, almond, peach seed, platycodon grandiflorum and green tea, adding 5-20 times of water by mass, soaking for 15-60 minutes, heating and refluxing for extraction for 0.5-2 hours, collecting first aromatic water, and filtering an extracting solution with 100 meshes to obtain a first extracting solution;

adding 5-20 times of water by mass into the medicine residues, performing reflux extraction for 0.5-2 hours, collecting second aromatic water, and filtering the extracting solution with 100 meshes to obtain a second extracting solution;

mixing and concentrating the first aromatic water and the second aromatic water, and/or mixing and concentrating the first extracting solution and the second extracting solution, and performing preparation processing to obtain the traditional Chinese medicine composition for adjuvant treatment of the middle stage of neocoronary infection.

The traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronary infection is safe and effective, and has the effects of clearing away heat and toxic materials, purging the lung and relieving asthma. The conditioning rule of the traditional Chinese medicine composition for adjuvant therapy of the middle-stage neocoronary infection is to clear away heat and toxic materials, purge the lung and relieve asthma, and aims at clinical phenotypes such as high fever, body heat retention, cough with little phlegm, yellow phlegm, abdominal distension and constipation. Chest oppression, shortness of breath, cough, dyspnea, and dyspnea with exertion. The effect is good for patients with red tongue, yellow greasy or yellow dry coating and smooth and rapid pulse. Solves the current situation that no related preventive products exist at present, is an accurate medicine-food homologous product with clear effective components and relatively clear action targets, and meets the clinical requirements and people needs of medium-term prevention and treatment.

Drawings

Fig. 1 is a network diagram of the association between traditional Chinese medicine raw materials-active ingredients-action targets of the traditional Chinese medicine composition for adjuvant therapy of metaphase neocoronary infection provided by the embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The embodiment of the invention provides a traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronal infection, which is prepared from the following raw materials in parts by weight:

1 to 30 parts of kudzu-vine root,

1 to 10 parts of gardenia, and the like,

1-15 parts of honeysuckle flower,

2-30 parts of houttuynia cordata,

1-20 parts of sea buckthorn leaves,

1-15 parts of perillaseed,

1-12 parts of almond, namely almond,

1 to 10 parts of peach kernel,

1-10 parts of platycodon grandiflorum,

1-10 parts of green tea.

The main effective chemical components of the traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronal infection comprise puerarin, geniposide, quercetin, apigenin, luteolin, kaempferol, eriodictyol (yellow), houttuynin, chlorogenic acid, corosolic acid, amygdalin, platycodin, tea polyphenol and the like. The kudzu root has obvious functions of relieving fever, improving human body microcirculation, resisting inflammation and the like, mainly comprises functional components of kudzu root isoflavone, and has prominent puerarin function, so that the kudzu root can dilate skin blood vessels, promote blood circulation and increase heat dissipation. Puerarin is mainly caused by blocking beta receptor of central related partcAMP production, inhibition of PGE2 and elevation of cAMP levels in the hypothalamus, and thus antipyretic effect. Can also inhibit body temperature rise by influencing the expression of heat shock protein messenger RNA in heat stress induced cells. Puerarin has anti-inflammatory effect by inhibiting the expression of related inflammatory factors in vivo, such as interleukin-1, interleukin-6, transforming growth factor-beta, etc., and inhibiting the action of inflammatory chemokines, such as MCP1 and TGF-beta. Researches show that puerarin can obviously reduce the mRNA level of a gene cox-2 at the upstream of inflammatory reaction, and that puerarin can inhibit the inflammatory reaction by influencing cox-2, further influencing the metabolism of arachidonic acid and prostaglandin. Geniposide is the most important active ingredient in iridoid of gardenia, and moreover, gardenia contains abundant organic acids, wherein chlorogenic acid and ursolic acid are more in content. Geniposide can inhibit early edema and exudation of inflammation, and inhibit tissue proliferation and granulation tissue generation at late stage of inflammation, genipin can effectively inhibit NO release induced by LPS in rat brain glioma cells, reduce tumor necrosis factor alpha, interleukin beta, prostaglandin E2, cell active oxygen content and NF-kB activity, and inhibit NO release caused by interferon gamma and beta amyloidosis. In addition, the geniposide obviously relieves the lung inflammatory infiltration of an influenza A H1N1 virus model mouse in vivo, and obviously reduces the virus titer in lung tissues, thereby effectively protecting the attacking effect of the virus on the mouse lung. The honeysuckle can inhibit inflammatory exudation and inflammatory hyperplasia, and the quercetin and the chlorogenic acid participate in regulating NF-kB, reducing IL-6, IL-10 and TNF-alpha inflammatory factors and inhibiting inflammatory reaction. Quercetin also inhibits viral infection and replication by inhibiting RdRp enzyme activity, while apigenin, luteolin, and quercetin inhibit 3CL pro activity, thereby inhibiting the binding of RNA to viral polymerase. The volatile oil is the main effective component of herba Houttuyniae, and decanoyl acetaldehyde (houttuynin) is the main effective component with antibacterial and antiinflammatory effects. The herba Houttuyniae volatile oil can inhibit LPS treated mouse macrophage from releasing PGE2, reduce mRNA transcription of cyclooxygenase-2 (COX-2) and COX-2 protein expression, regulate COX-2 enzyme to exert antiinflammatory effect, and remarkably inhibit COX-2 enzymeSARS coronavirus 3CL protease (3CLpro) is produced. Corosolic acid exerts an anti-inflammatory effect by inhibiting the expression of tumor necrosis factor-alpha (TNF-alpha), IL-6, Inducible Nitric Oxide Synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-KB inflammatory factors. The fatty oil extracted from fructus Perillae has obvious effects of relieving cough and asthma, and has the effect of 0.05 g/kg^-1Like azatheophylline, fatty acids in perilla oil have antiallergic and antiinflammatory effects, and the mechanism is related to inhibiting Platelet Activating Factor (PAF) and leukotriene production, and can significantly reduce the production of proinflammatory factors IL-lB and IL-6, thereby relieving inflammation. Amygdalin in semen Armeniacae amarum and semen Persicae is decomposed in human body to generate trace amount of hydrocyanic acid, which can inhibit respiratory center to make respiratory movement smooth, thereby relieving cough and asthma. Platycodin D is a main cough-relieving active ingredient, so that the amount of airway secretion liquid is increased, thick sputum attached to airway mucosa is thinned, and the sputum falls off from airway walls, thereby achieving the effect of eliminating phlegm. The green tea polyphenol is a special substance in green tea, can obviously inhibit the activity of 3CLPro protease of SARS virus, inhibits the virus from replicating in a host, and has stronger antiviral effect. In addition, tea polyphenols and oligomannose can remove active oxygen free radicals from the body, prevent diseases and reduce oxidative damage. Therefore, various main chemical components in the traditional Chinese medicine composition for the auxiliary treatment of the middle-stage new crown infection have definite functions of resisting virus, relieving fever and inflammation, relieving cough and asthma and the like, play a role through the functions of multiple components, multiple targets and multiple channels, and provide scientific basis for the clinical application and the product development of the traditional Chinese medicine composition. The formula is mainly suitable for early stages of pneumonia caused by epidemic toxin blocking lung, epidemic pathogen blocking and heat toxin blocking lung, and related modern pharmacological studies show that the formula has the biological activities of defervescence, cough relieving, asthma relieving, inflammation resisting, virus resisting and the like. As shown in figure 1, the systematic research result of the network pharmacology of the formula based on big data shows that many components can effectively resist new corolla pneumonitis virus and relieve the related symptoms caused by the new corolla pneumonitis virus, and the medicinal diet is an accurate medicinal diet product with clear main components and definite target spots and fills up the blank of related products.

Preferably, the green tea is Biluochun tea.

Preferably, the raw materials for preparing the traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronal infection also comprise 1-5 parts of oligomannose. The low-mannose can directly enter into large intestine to be utilized by Bacillus bifidus, and promote proliferation of Bacillus bifidus and simultaneously generate multiple organic acids. The pH value of the intestinal tract is reduced, the growth of harmful bacteria is inhibited, and the probiotics are greatly proliferated in the intestinal tract, so that the intestinal function is adjusted.

More preferably, the traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronary infection is prepared from the following raw materials in parts by weight: 14-16 parts of kudzu vine root, 9-10 parts of cape jasmine fruit, 9-11 parts of honeysuckle flower, 14-16 parts of houttuynia cordata, 8-11 parts of sea buckthorn leaf, 8-11 parts of perillaseed, 11-12 parts of almond, 9-10 parts of peach kernel, 5-7 parts of platycodon grandiflorum and 4-6 parts of green tea. More specifically, the traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronal infection is prepared from the following raw materials in parts by weight: 15 parts of kudzu root, 10 parts of cape jasmine fruit, 10 parts of honeysuckle flower, 15 parts of cordate houttuynia, 10 parts of sea buckthorn leaf, 10 parts of perillaseed, 12 parts of almond, 10 parts of peach kernel, 6 parts of platycodon root and 5 parts of green tea.

The specific functions of the components are shown in the table 1:

TABLE 1

The traditional Chinese medicine composition for adjuvant therapy of the middle-stage neocoronary infection has the characteristics of definite curative effect, convenience in use, clear action target and pharmacological effect, wide raw material source, low cost, simple preparation method, high patient compliance and the like, is a product researched by an inventor aiming at patients infected with the middle-stage neocoronary pneumonia (common type, epidemic toxin blocking lung-epidemic pathogen block and heat toxin blocking lung), and solves the problem that no related medicine and food homologous products exist at present. The nation summarizes good experience in the epidemic battle, the effect of the traditional Chinese medicine is recognized by people again, and great contribution is made to the guarantee of life and property safety of people; according to the guidance of a novel diagnosis and treatment scheme (trial seventh edition) for coronavirus pneumonia, during clinical treatment, lung-clearing and toxin-expelling decoction is recommended to be used, so that the decoction is suitable for light, ordinary and heavy patients, and no related oral Chinese patent medicines can be selected. The new coronavirus pneumonia is emergent in emergency and is a public health accident; at present, no traditional Chinese medicine health product or health product which is available for preventing and treating the new coronavirus pneumonia exists. Particularly, the medicine is lack of effective medicine and food homologous anti-new coronary pneumonia virus products for patients in middle period (common type, epidemic toxin blocking lung-epidemic pathogen blocking and heat toxin blocking lung). In order to fully exert the prevention and treatment advantages of the traditional Chinese medicine, a series of safe and effective accurate medicated diet with reliable quality, clear effective components and clear action targets for early prevention, middle-term treatment and later-term rehabilitation is developed. The traditional Chinese medicine composition for adjuvant therapy of the middle-stage neocoronary infection aims at common patients in the middle stage, and has the clinical manifestations of high fever, body heat retention, cough with little phlegm, or yellow phlegm, abdominal distension and constipation. Chest oppression, shortness of breath, cough, dyspnea, and dyspnea with exertion. The product has the advantages of red tongue, yellow and greasy or yellow and dry tongue coating and smooth and rapid pulse, so the development of the product for resisting the new coronary pneumonia virus has very important significance and large market demand.

The embodiment of the invention also provides a preparation method of the traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronal infection, which comprises the following steps:

weighing the raw materials according to the weight parts of the traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronal infection, mixing kudzu vine root, cape jasmine fruit, honeysuckle flower, houttuynia cordata, sea backthern, almond, peach seed, platycodon grandiflorum and green tea, adding 5-20 times of water by mass, soaking for 15-60 minutes, heating and refluxing for 0.5-2 hours, collecting first aromatic water, and filtering an extracting solution with a sieve of 80-200 meshes to obtain a first extracting solution;

adding 5-20 times of water by mass into the medicine residues, performing reflux extraction for 0.5-2 hours, collecting second aromatic water, and filtering the extracting solution by a sieve of 80-200 meshes to obtain a second extracting solution;

Preferably, radix puerariae, fructus gardeniae, honeysuckle, houttuynia cordata, sea buckthorn leaves, perillaseed, almonds, peach kernels, platycodon grandiflorum and green tea are mixed, water in an amount which is 8-15 times the mass of the mixture is added for soaking for 20-40 minutes, heating and reflux extraction is carried out for 0.5-2 hours, and 100-mesh filtration is carried out to obtain a first extracting solution;

adding 8-15 times of water by mass into the medicine residues, heating, refluxing and extracting for 0.5-2 hours, and filtering by a 100-mesh sieve to obtain a second extracting solution.

Specifically, the traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronal infection can be prepared into a liquid preparation, and specifically, the first extract and the second extract are concentrated under reduced pressure to a density of about 1.02 to obtain a concentrated solution for later use; adding aromatic water and Tween 80 into the concentrated solution, stirring, adding flavoring agent, centrifuging to obtain clear liquid, packaging, and making into oral liquid, beverage, etc.

Specifically, mixing the first extractive solution and the second extractive solution, concentrating at 70 deg.C under reduced pressure to relative density of about 1.08 to obtain concentrated solution, spray drying, and preparing spray dried powder; collecting aromatic water, adding BETA-cyclodextrin 5, cooling, refrigerating for 24 hr, filtering to obtain clathrate, and drying at 60 deg.C to obtain aromatic water clathrate. Adding the above spray-dried powder, and mixing with mannose oligomer; subpackaging with 10g per bag to obtain the solid beverage.

In addition, the spray dried powder can be mixed with appropriate food materials and adjuvants such as mannan oligosaccharide, granulating, drying, packaging, and making into granule at a weight of 10 g/bag.

Or spraying dry powder, adding appropriate food materials and adjuvants such as mannan oligosaccharide, granulating, drying, and tabletting; or adopting appropriate adjuvants such as maltodextrin, corn starch, wheat flour, and protein powder, performing dry granulation or directly tabletting to obtain appropriate tablet.

The final form of the product can be various, including powder, granule, tablet, honeyed pill, soft extract, oral liquid, syrup, etc. In the preparation process, the drying mode can be spray drying, vacuum drying, freeze drying and the like; the granulation method can be dry granulation, wet granulation, one-step granulation and the like. Preferably, the concentrated solution is dried under reduced pressure, dried by spraying, and quickly dried to obtain powder, so that damage to various components is reduced, and support is provided for effectiveness of the product.

By adopting the method acceptable by relevant laws and regulations of pharmacy, food, health care products and the like, the traditional Chinese medicine components of the traditional Chinese medicine composition for the auxiliary treatment of the middle-stage neocoronary infection can be extracted, concentrated, dried or crushed, further processed into products, the effective components of the compound are reserved, and the products are prepared into suitable administration forms (including dosage forms such as granules, powder, oral liquid, tablets and the like) or food forms such as tablet candies, meal powder, solid beverages and the like, so that the requirements of the market on relevant products are met, and the invention belongs to the protection scope.

The above-mentioned traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronal infection and the preparation method thereof are described below by way of specific formulation and preparation process examples.

Example 1:

selecting raw materials according to the weight part of a traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronal infection, mixing 7.5kg of kudzuvine root, 5kg of cape jasmine fruit, 5kg of honeysuckle flower, 7.5kg of heartleaf houttuynia herb, 5kg of sea buckthorn leaf, 5kg of perilla fruit, 6kg of almond, 5kg of peach seed, 3kg of platycodon root and 2.5kg of green tea, adding 515L of water for soaking for 30 minutes, heating and refluxing for extraction for 45 minutes, filtering by 100 meshes, collecting 49L of aromatic water, filtering the extracting solution to obtain a first extracting solution, adding 430L of water for refluxing for extraction for 45 minutes, filtering by 100 meshes, collecting 49L of aromatic water, and filtering the extracting solution to obtain a second extracting solution. Mixing the first extractive solution and the second extractive solution, concentrating under reduced pressure to density of about 1.02, adding 98L of aromatic water, adding Tween 80, stirring, adding flavoring agent, centrifuging to obtain clear liquid, packaging, and making into oral liquid.

Example 2:

10 patients infected with the middle-stage new coronary pneumonia are selected, the medicinal and edible formula oral liquid for adjuvant therapy of the middle-stage new coronary pneumonia is taken every day for two weeks, no adverse reaction occurs, and the illness state of 7 patients is obviously relieved.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. The traditional Chinese medicine composition for adjuvant therapy of the middle stage of neocoronal infection is characterized by being prepared from the following raw materials in parts by weight:

1 to 30 parts of kudzu-vine root,

1 to 10 parts of gardenia, and the like,

1-15 parts of honeysuckle flower,

2-30 parts of houttuynia cordata,

1-20 parts of sea buckthorn leaves,

1-15 parts of perillaseed,

1-12 parts of almond, namely almond,

1 to 10 parts of peach kernel,

1-10 parts of platycodon grandiflorum,

1-10 parts of green tea.

2. The traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronary infection according to claim 1, which is prepared from the following raw materials in parts by weight:

14-16 parts of kudzu-vine root,

9-10 parts of cape jasmine fruit,

9-11 parts of honeysuckle flower,

14-16 parts of houttuynia cordata,

8-11 parts of sea buckthorn leaves,

8-11 parts of perillaseed,

11-12 parts of almond, namely almond,

9-10 parts of peach kernel,

5-7 parts of platycodon grandiflorum,

4-6 parts of green tea.

3. The traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronary infection according to claim 2, which is prepared from the following raw materials in parts by weight:

15 parts of kudzu-vine root,

10 parts of cape jasmine fruit, wherein the jasmine fruit is,

10 parts of honeysuckle flower, including (by weight portion),

15 parts of houttuynia cordata, wherein,

10 parts of sea buckthorn leaves, namely 10 parts of,

10 parts of Perilla fruit, namely Perilla fruit,

12 parts of almond, namely 12 parts of almond,

10 parts of peach kernel, namely 10 parts of,

6 parts of platycodon grandiflorum, namely,

5 parts of green tea.

4. The traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronary infection according to claim 1, wherein the raw materials for preparing the traditional Chinese medicine composition further comprise 1-5 parts of mannose oligomer.

5. A preparation method of a traditional Chinese medicine composition for adjuvant therapy of middle-stage neocoronary infection is characterized by comprising the following steps:

weighing the raw materials according to the weight parts of the traditional Chinese medicine composition for adjuvant therapy of middle stage neocoronal infection according to any one of claims 1 to 3, mixing kudzu root, gardenia, honeysuckle, houttuynia cordata, sea buckthorn leaf, perilla fruit, almond, peach kernel, platycodon grandiflorum and green tea, adding 5-20 times of water by mass, soaking for 15-60 minutes, heating and refluxing for extraction for 0.5-2 hours, collecting first aromatic water, and filtering the extract with 80-200 meshes to obtain a first extract;

6. The method for preparing the traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronary infection according to claim 5, wherein the radix puerariae, the gardenia, the honeysuckle, the houttuynia cordata, the sea buckthorn leaves, the perilla seeds, the almonds, the peach kernels, the platycodon grandiflorum and the green tea are mixed, and the mixture is soaked in water in an amount of 8-15 times by mass for 20-40 minutes.

7. The preparation method of the traditional Chinese medicine composition for the adjuvant therapy of the middle stage of neocoronary infection according to claim 5, wherein 8-15 times of water by mass is added into the medicine residue.

8. The method for preparing the Chinese medicinal composition for the adjuvant treatment of the middle-stage neocoronary infection according to claim 5, wherein the mannose oligomer is added during the preparation process.