CN111918972A - 评估存在或不存在有复制能力的病毒的方法和试剂 - Google Patents

评估存在或不存在有复制能力的病毒的方法和试剂 Download PDF

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Publication number
CN111918972A
CN111918972A CN201980021932.5A CN201980021932A CN111918972A CN 111918972 A CN111918972 A CN 111918972A CN 201980021932 A CN201980021932 A CN 201980021932A CN 111918972 A CN111918972 A CN 111918972A
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seq
sequence
gene
oligonucleotide primer
viral
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Chinese (zh)
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R·贝瑞
E·韦伯
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Juno Therapeutics Inc
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Juno Therapeutics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/161—HIV-1 ; HIV-2 gag-pol, e.g. p55, p24/25, p17/18, p7, p6, p66/68, p51/52, p31/34, p32, p40
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/162—HIV-1 ; HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, CD4-Binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Communicable Diseases (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980021932.5A 2018-01-31 2019-01-31 评估存在或不存在有复制能力的病毒的方法和试剂 Pending CN111918972A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862624801P 2018-01-31 2018-01-31
US62/624,801 2018-01-31
PCT/US2019/016194 WO2019152747A1 (en) 2018-01-31 2019-01-31 Methods and reagents for assessing the presence or absence of replication competent virus

Publications (1)

Publication Number Publication Date
CN111918972A true CN111918972A (zh) 2020-11-10

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US (2) US11535903B2 (https=)
EP (1) EP3746569A1 (https=)
JP (1) JP2021511802A (https=)
CN (1) CN111918972A (https=)
WO (1) WO2019152747A1 (https=)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112505126A (zh) * 2020-12-16 2021-03-16 中国科学院重庆绿色智能技术研究院 金属离子在影响诱导HIV-1的Tat蛋白水解中的应用及其检测方法
CN112877475A (zh) * 2021-02-19 2021-06-01 北京鼎成肽源生物技术有限公司 一种检测rcl的引物探针组合、试剂盒和检测方法
CN113403343A (zh) * 2021-04-30 2021-09-17 吉林大学 一种h3n2与h9n2亚型禽流感二价嵌合型病毒样颗粒的制备
CN113699278A (zh) * 2021-09-17 2021-11-26 深圳因诺免疫有限公司 一种基于q-PCR法检测复制型逆转录病毒的引物、试剂盒及检测方法
CN114958983A (zh) * 2021-11-04 2022-08-30 湖州申科生物技术有限公司 分析和检测慢病毒载体介导的目的基因的拷贝数的方法
CN117737303A (zh) * 2023-12-14 2024-03-22 浙江恒驭生物科技有限公司 细胞治疗产品中复制型逆转录病毒rcr-galv的检测组合物、方法及试剂盒
TWI852072B (zh) * 2022-08-08 2024-08-11 新加坡商台達電子國際（新加坡）私人有限公司 檢測病毒感染性的樣本前處理試劑組合及方法

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JP7483373B2 (ja) 2016-07-29 2024-05-15 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法
US11535903B2 (en) 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
CN111910015A (zh) * 2019-05-08 2020-11-10 深圳宾德生物技术有限公司 一种检测复制型慢病毒的探针、引物对、荧光定量pcr试剂盒及方法
KR20220080172A (ko) * 2019-10-11 2022-06-14 카탈로그 테크놀로지스, 인크. 핵산 보안 및 인증
GB202003412D0 (en) 2020-03-09 2020-04-22 Oxford Biomedica Ltd Replication competent virus assay
CN113528706A (zh) * 2021-07-13 2021-10-22 苏州博腾生物制药有限公司 三通道双淬灭探针检测可复制性慢病毒的试剂及其检测方法
CN114410844A (zh) * 2022-01-29 2022-04-29 宁波熙宁检测技术有限公司 多重qPCR检测GALV和ALB拷贝数判定CAR-T细胞产品中RCR阴阳性的方法
CN114369687A (zh) * 2022-01-29 2022-04-19 宁波熙宁检测技术有限公司 同时检测VSVG和ALB基因的多重qPCR反应体系的建立方法
KR102804750B1 (ko) * 2022-10-20 2025-05-12 성균관대학교산학협력단 바이러스 생산 세포 검출용 멀티플렉스 pcr 프라이머 및 프로브
CN116219069A (zh) * 2022-11-11 2023-06-06 湖州申科生物技术股份有限公司 一种检测可复制性慢病毒的探针、引物、试剂盒及其应用
CN116970737A (zh) * 2023-04-27 2023-10-31 北京艺升医疗科技有限公司 一种复制型慢病毒检测方法

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112505126A (zh) * 2020-12-16 2021-03-16 中国科学院重庆绿色智能技术研究院 金属离子在影响诱导HIV-1的Tat蛋白水解中的应用及其检测方法
CN112505126B (zh) * 2020-12-16 2023-03-24 中国科学院重庆绿色智能技术研究院 检测金属离子影响诱导HIV-1的Tat蛋白水解的方法
CN112877475A (zh) * 2021-02-19 2021-06-01 北京鼎成肽源生物技术有限公司 一种检测rcl的引物探针组合、试剂盒和检测方法
CN113403343A (zh) * 2021-04-30 2021-09-17 吉林大学 一种h3n2与h9n2亚型禽流感二价嵌合型病毒样颗粒的制备
CN113699278A (zh) * 2021-09-17 2021-11-26 深圳因诺免疫有限公司 一种基于q-PCR法检测复制型逆转录病毒的引物、试剂盒及检测方法
CN114958983A (zh) * 2021-11-04 2022-08-30 湖州申科生物技术有限公司 分析和检测慢病毒载体介导的目的基因的拷贝数的方法
TWI852072B (zh) * 2022-08-08 2024-08-11 新加坡商台達電子國際（新加坡）私人有限公司 檢測病毒感染性的樣本前處理試劑組合及方法
CN117737303A (zh) * 2023-12-14 2024-03-22 浙江恒驭生物科技有限公司 细胞治疗产品中复制型逆转录病毒rcr-galv的检测组合物、方法及试剂盒

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EP3746569A1 (en) 2020-12-09
US12264371B2 (en) 2025-04-01
WO2019152747A1 (en) 2019-08-08
US20210164062A1 (en) 2021-06-03
US20230193411A1 (en) 2023-06-22
US11535903B2 (en) 2022-12-27
JP2021511802A (ja) 2021-05-13

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