JP2021511802A - 複製可能ウイルスの存在または非存在を評価するための方法および試薬 - Google Patents

複製可能ウイルスの存在または非存在を評価するための方法および試薬 Download PDF

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JP2021511802A
JP2021511802A JP2020541756A JP2020541756A JP2021511802A JP 2021511802 A JP2021511802 A JP 2021511802A JP 2020541756 A JP2020541756 A JP 2020541756A JP 2020541756 A JP2020541756 A JP 2020541756A JP 2021511802 A JP2021511802 A JP 2021511802A
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seq
gene
sequence
specific
oligonucleotide primer
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JPWO2019152747A5 (https=
JP2021511802A5 (https=
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ルース ベリー
ルース ベリー
エドウィン ウェブ
エドウィン ウェブ
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ジュノー セラピューティクス インコーポレイテッド
ジュノー セラピューティクス インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/161—HIV-1 ; HIV-2 gag-pol, e.g. p55, p24/25, p17/18, p7, p6, p66/68, p51/52, p31/34, p32, p40
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/162—HIV-1 ; HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, CD4-Binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Communicable Diseases (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2020541756A 2018-01-31 2019-01-31 複製可能ウイルスの存在または非存在を評価するための方法および試薬 Pending JP2021511802A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862624801P 2018-01-31 2018-01-31
US62/624,801 2018-01-31
PCT/US2019/016194 WO2019152747A1 (en) 2018-01-31 2019-01-31 Methods and reagents for assessing the presence or absence of replication competent virus

Publications (3)

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JP2021511802A true JP2021511802A (ja) 2021-05-13
JPWO2019152747A5 JPWO2019152747A5 (https=) 2022-02-02
JP2021511802A5 JP2021511802A5 (https=) 2022-02-02

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US (2) US11535903B2 (https=)
EP (1) EP3746569A1 (https=)
JP (1) JP2021511802A (https=)
CN (1) CN111918972A (https=)
WO (1) WO2019152747A1 (https=)

Families Citing this family (18)

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JP7483373B2 (ja) 2016-07-29 2024-05-15 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法
US11535903B2 (en) 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
CN111910015A (zh) * 2019-05-08 2020-11-10 深圳宾德生物技术有限公司 一种检测复制型慢病毒的探针、引物对、荧光定量pcr试剂盒及方法
KR20220080172A (ko) * 2019-10-11 2022-06-14 카탈로그 테크놀로지스, 인크. 핵산 보안 및 인증
GB202003412D0 (en) 2020-03-09 2020-04-22 Oxford Biomedica Ltd Replication competent virus assay
CN112505126B (zh) * 2020-12-16 2023-03-24 中国科学院重庆绿色智能技术研究院 检测金属离子影响诱导HIV-1的Tat蛋白水解的方法
CN112877475A (zh) * 2021-02-19 2021-06-01 北京鼎成肽源生物技术有限公司 一种检测rcl的引物探针组合、试剂盒和检测方法
CN113403343A (zh) * 2021-04-30 2021-09-17 吉林大学 一种h3n2与h9n2亚型禽流感二价嵌合型病毒样颗粒的制备
CN113528706A (zh) * 2021-07-13 2021-10-22 苏州博腾生物制药有限公司 三通道双淬灭探针检测可复制性慢病毒的试剂及其检测方法
CN113699278A (zh) * 2021-09-17 2021-11-26 深圳因诺免疫有限公司 一种基于q-PCR法检测复制型逆转录病毒的引物、试剂盒及检测方法
CN114958983B (zh) * 2021-11-04 2023-03-24 湖州申科生物技术股份有限公司 分析和检测慢病毒载体介导的目的基因的拷贝数的方法
CN114410844A (zh) * 2022-01-29 2022-04-29 宁波熙宁检测技术有限公司 多重qPCR检测GALV和ALB拷贝数判定CAR-T细胞产品中RCR阴阳性的方法
CN114369687A (zh) * 2022-01-29 2022-04-19 宁波熙宁检测技术有限公司 同时检测VSVG和ALB基因的多重qPCR反应体系的建立方法
CN117568446A (zh) * 2022-08-08 2024-02-20 台达电子国际（新加坡）私人有限公司 检测病毒感染性的样本前处理试剂盒及方法
KR102804750B1 (ko) * 2022-10-20 2025-05-12 성균관대학교산학협력단 바이러스 생산 세포 검출용 멀티플렉스 pcr 프라이머 및 프로브
CN116219069A (zh) * 2022-11-11 2023-06-06 湖州申科生物技术股份有限公司 一种检测可复制性慢病毒的探针、引物、试剂盒及其应用
CN116970737A (zh) * 2023-04-27 2023-10-31 北京艺升医疗科技有限公司 一种复制型慢病毒检测方法
CN117737303A (zh) * 2023-12-14 2024-03-22 浙江恒驭生物科技有限公司 细胞治疗产品中复制型逆转录病毒rcr-galv的检测组合物、方法及试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090028836A1 (en) * 2005-06-01 2009-01-29 Kalle Christof Von Stem and progenitor cell expansion by evi, evi-like genes and setbp1
WO2018023094A1 (en) * 2016-07-29 2018-02-01 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452773A (en) 1982-04-05 1984-06-05 Canadian Patents And Development Limited Magnetic iron-dextran microspheres
US5168062A (en) 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
ES2060765T3 (es) 1988-05-17 1994-12-01 Lubrizol Genetics Inc Sistema promotor de ubiquitina en plantas.
ES2067018T3 (es) 1988-12-28 1995-03-16 Stefan Miltenyi Procedimiento y materiales para la separacion en alto gradiente magnetico de materiales biologicos.
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5200084A (en) 1990-09-26 1993-04-06 Immunicon Corporation Apparatus and methods for magnetic separation
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
US5834256A (en) 1993-06-11 1998-11-10 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
WO1997034634A1 (en) 1996-03-20 1997-09-25 Sloan-Kettering Institute For Cancer Research Single chain fv constructs of anti-ganglioside gd2 antibodies
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
EP0961830A1 (en) 1997-01-29 1999-12-08 Neurosearch A/S EXPRESSION VECTORS AND METHODS FOR $i(IN VIVO) EXPRESSION OF THERAPEUTIC POLYPEPTIDES
US6207455B1 (en) 1997-05-01 2001-03-27 Lung-Ji Chang Lentiviral vectors
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
JP2002524081A (ja) 1998-09-04 2002-08-06 スローン − ケッタリング インスティチュート フォー キャンサー リサーチ 前立腺−特異的膜抗原に特異的な融合受容体およびその使用
WO2000023573A2 (en) 1998-10-20 2000-04-27 City Of Hope Cd20-specific redirected t cells and their use in cellular immunotherapy of cd20+ malignancies
US20020131960A1 (en) 2000-06-02 2002-09-19 Michel Sadelain Artificial antigen presenting cells and methods of use thereof
ATE332398T1 (de) 2000-08-11 2006-07-15 Univ Utah Res Found Einfach markierte oligonukleotidsonden
AU2001297703B2 (en) 2000-11-07 2006-10-19 City Of Hope CD19-specific redirected immune cells
US6712612B1 (en) 2000-12-12 2004-03-30 Genecure Llc Safe and stable retroviral helper cell line and related compositions and methods
US7070995B2 (en) 2001-04-11 2006-07-04 City Of Hope CE7-specific redirected immune cells
US20090257994A1 (en) 2001-04-30 2009-10-15 City Of Hope Chimeric immunoreceptor useful in treating human cancers
KR100440852B1 (ko) 2001-05-10 2004-07-19 주식회사 바이로메드 레트로바이러스에 특이적인 올리고뉴클레오티드 프라이머및 이를 이용한 레트로바이러스 농도 측정방법
US7446190B2 (en) 2002-05-28 2008-11-04 Sloan-Kettering Institute For Cancer Research Nucleic acids encoding chimeric T cell receptors
JP4992032B2 (ja) 2002-09-03 2012-08-08 オックスフォード バイオメディカ（ユーケー）リミテッド レトロウイルスベクター
US20050129671A1 (en) 2003-03-11 2005-06-16 City Of Hope Mammalian antigen-presenting T cells and bi-specific T cells
US7435596B2 (en) 2004-11-04 2008-10-14 St. Jude Children's Research Hospital, Inc. Modified cell line and method for expansion of NK cell
WO2007008309A2 (en) 2005-06-01 2007-01-18 Children's Hospital Medical Center Stem and progenitor cell expansion by evi, evi-like genes and setbp1
WO2007039301A2 (en) 2005-10-05 2007-04-12 Roche Diagnostics Gmbh Non-fluorescent energy transfer
CA2682527C (en) 2007-03-30 2017-07-11 Memorial Sloan-Kettering Cancer Center Constitutive expression of costimulatory ligands on adoptively transferred t lymphocytes
WO2009072003A2 (en) 2007-12-07 2009-06-11 Miltenyi Biotec Gmbh Sample processing system and methods
US8479118B2 (en) 2007-12-10 2013-07-02 Microsoft Corporation Switching search providers within a browser search box
RS56844B1 (sr) 2007-12-11 2018-04-30 Univ North Carolina Chapel Hill Retrovirusni vektori sa modifikovanim polipurinskim nizom
US20120164718A1 (en) 2008-05-06 2012-06-28 Innovative Micro Technology Removable/disposable apparatus for MEMS particle sorting device
JP5173594B2 (ja) 2008-05-27 2013-04-03 キヤノン株式会社 管理装置、画像形成装置及びそれらの処理方法
CN102154368B (zh) 2010-02-11 2015-01-21 上海比昂生物医药科技有限公司 一种组织特异性兼可调控性慢病毒基因表达载体
PH12013501201A1 (en) 2010-12-09 2013-07-29 Univ Pennsylvania Use of chimeric antigen receptor-modified t cells to treat cancer
CN110200997A (zh) 2011-03-23 2019-09-06 弗雷德哈钦森癌症研究中心 用于细胞免疫治疗的方法和组合物
US8398282B2 (en) 2011-05-12 2013-03-19 Delphi Technologies, Inc. Vehicle front lighting assembly and systems having a variable tint electrowetting element
KR102134932B1 (ko) 2011-11-11 2020-07-17 프레드 헛친슨 켄서 리서치 센터 암을 위한 사이클린 ａ１―표적화된 ｔ―세포 면역요법
JP6850528B2 (ja) 2012-02-13 2021-03-31 シアトル チルドレンズ ホスピタル ドゥーイング ビジネス アズ シアトル チルドレンズ リサーチ インスティテュート 二重特異性キメラ抗原受容体およびその治療的使用
WO2013126726A1 (en) 2012-02-22 2013-08-29 The Trustees Of The University Of Pennsylvania Double transgenic t cells comprising a car and a tcr and their methods of use
SG10201609210SA (en) 2012-05-03 2016-12-29 Hutchinson Fred Cancer Res Enhanced affinity t cell receptors and methods for making the same
AU2013289979A1 (en) * 2012-07-13 2015-01-22 The Trustees Of The University Of Pennsylvania Methods of assessing the suitability of transduced T cells for administration
CA2881981A1 (en) 2012-08-20 2014-02-27 Fred Hutchinson Cancer Research Center Method and compositions for cellular immunotherapy
AU2013327136A1 (en) 2012-10-02 2015-04-16 Memorial Sloan-Kettering Cancer Center Compositions and methods for immunotherapy
US10072273B2 (en) * 2014-01-10 2018-09-11 Helmholtz Zentrum München—Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Pseudotyped lentiviral vectors
US11535903B2 (en) * 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090028836A1 (en) * 2005-06-01 2009-01-29 Kalle Christof Von Stem and progenitor cell expansion by evi, evi-like genes and setbp1
WO2018023094A1 (en) * 2016-07-29 2018-02-01 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MOLECULAR THERAPY - METHODS & CLINICAL DEVELOPMENT, vol. 8, JPN5021006218, 2017, pages 1 - 7, ISSN: 0005162157 *
MOLECULAR THERAPY, vol. 19, JPN6022054873, 2010, pages 557 - 566, ISSN: 0005162160 *
MOLECULAR THERAPY, vol. 26, JPN5021006214, 2017, pages 269 - 279, ISSN: 0005162158 *
MOLECULAR THERAPY, vol. 8, JPN6022054874, 2003, pages 830 - 839, ISSN: 0005162159 *

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EP3746569A1 (en) 2020-12-09
CN111918972A (zh) 2020-11-10
US12264371B2 (en) 2025-04-01
WO2019152747A1 (en) 2019-08-08
US20210164062A1 (en) 2021-06-03
US20230193411A1 (en) 2023-06-22
US11535903B2 (en) 2022-12-27

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