CN111876456A - 一种黑水虻抗菌肽的提取方法 - Google Patents

一种黑水虻抗菌肽的提取方法 Download PDF

Info

Publication number
CN111876456A
CN111876456A CN202010708205.0A CN202010708205A CN111876456A CN 111876456 A CN111876456 A CN 111876456A CN 202010708205 A CN202010708205 A CN 202010708205A CN 111876456 A CN111876456 A CN 111876456A
Authority
CN
China
Prior art keywords
antibacterial peptide
hermetia illucens
larvae
aspergillus oryzae
preparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010708205.0A
Other languages
English (en)
Inventor
刘德江
刘一
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN202010708205.0A priority Critical patent/CN111876456A/zh
Publication of CN111876456A publication Critical patent/CN111876456A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • C07K1/303Extraction; Separation; Purification by precipitation by salting out
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

本发明涉及一种黑水虻抗菌肽的提取方法。包括:用米曲霉种子液与乳酸杆菌制备发酵液;用有机废弃物制备黑水虻养殖料;制备成发酵养殖料;黑水虻幼虫在发酵养殖料中养殖到5龄幼虫;黑水虻5龄幼虫与发酵养殖料分离;幼虫及发酵液一起粉碎均质得到降解液;离心去除非蛋白杂质得到降解物的上清液;上清液以三氯乙酸沉淀大分子蛋白,再次离心得去大分子上清液;用盐酸溶液洗脱吸附在海藻酸上的抗菌肽;用氯化钠沉析,透析,脱盐干燥得抗菌肽粗品;用超滤膜过滤得超滤液,冰冻干燥得目标抗菌肽。本发明应用低成本黑水虻制备广谱抗菌肽,成本低,适于大规模生产;抗菌肽为粗品,可以作为抗菌肽原料药;利用垃圾养殖黑水虻,使垃圾变废为宝。

Description

一种黑水虻抗菌肽的提取方法
技术领域
本发明涉及一种黑水虻抗菌肽的提取方法,属于医药生物技术领域。
背景技术
黑水虻处理畜禽粪便、餐饮垃圾、蔬菜水果垃圾、作物秸秆等有机废弃物技术已得到广泛利用,而处理有机废弃物过程会生产大量黑水虻幼虫。
黑水虻不具备高等动物那样的免疫体系,以产生抗菌肽的方式构建免疫系统,抑制和杀灭入侵的微生物。
抗菌肽通常是由多个氨基酸残基组成的小分子多肽,具有较强的热稳定性、水溶性以及广谱高效的抗菌活性。抗菌肽的抗菌机理与抗生素不同,不易产生耐药性菌株。抗菌肽不仅对革兰阳性菌和革兰阴性菌有较强的抑菌作用,对某些真菌、病毒、癌细胞也具有杀灭作用。
利用黑水虻为原料提取抗菌肽是一条经济便捷的途径。
CN202010094406.6公开了一种黑水虻抗菌肽的提取方法及其在制备抗烟草病害的肥料中的应用,其利用蘸有烟草青枯病菌、烟草野火病菌和烟草角斑病菌诱导菌液的针刺破幼虫腹部后将其放入诱导菌液中浸泡产生抗菌肽。黑水虻虫体较小,针刺虫体在大规模的生产中难以实现。另外,诱导菌烟草青枯病菌、烟草野火病菌和烟草角斑病菌都是烟草有害菌,生产中很难防止有害菌的扩散,且防止措施成本相对较高。该申请方案的目的是生产防抗烟草青枯病菌、烟草野火病菌和烟草角斑病菌的肥料,应用领域较窄,限制了黑水虻抗菌肽的应用领域。
发明内容
本发明要解决的技术问题是提供一种黑水虻抗菌肽的提取方法,该方法以处理有机废弃物过程中生产的大量黑水虻幼虫为原料,经济,便捷。
本发明解决其技术问题采用的技术方案是:
一种黑水虻抗菌肽的提取方法,按下述步骤进行:
(1)按米曲霉制种方法制备一定量的米曲霉种子液,米曲霉种子液加入乳酸杆菌扩大培养24小时成为发酵液,发酵液中乳酸杆菌菌体密度2-4亿个/升;
(2)用有机废弃物制备黑水虻养殖料,有机废弃物包括畜禽粪便、餐饮垃圾、蔬菜水果垃圾、作物秸秆;
(3)接种米曲霉种子液于黑水虻养殖料中,米曲霉种子液与黑水虻养殖料的质量比为1:10-20,制备成发酵养殖料;
(4)将黑水虻幼虫投入步骤(3)制备的发酵养殖料中,控制养殖环境温度25-32℃,湿度65-75%,养殖幼虫达到5龄幼虫;
(5)将步骤(4)养殖的黑水虻5龄幼虫与发酵养殖料分离,将5龄幼虫置于步骤(1)制备的发酵液中再养殖24小时,米曲霉和乳酸杆菌诱导黑水虻产生更多抗菌肽;
(6)将步骤(5)养殖24小时的幼虫及发酵液一起置于胶体磨,粉碎均质10-20分钟后置于恒温反应器中,30℃下反应12-36小时得到降解液,米曲霉蛋白酶会将黑水虻部分大分子蛋白降解为小分子的类抗菌肽;
(7)将步骤(6)得到的降解液用10000转/分,离心去除非蛋白杂质,得到降解物的上清液;
(8)降解物上清液以0.5mol/L三氯乙酸沉淀大分子蛋白,静置30分钟后10000转/分再次离心,得到去大分子上清液;
(9)去大分子上清液用海藻酸过滤吸附,再用0.2mol/L盐酸溶液洗脱吸附在海藻酸上的抗菌肽;
(10)将洗脱液用氢氧化钠调解PH值为4.5后,用氯化钠沉析,透析,脱盐干燥得抗菌肽粗品;
(11)将抗菌肽粗品用超纯水溶解,经过0.22μm-0.45μm超滤膜过滤,得到超滤液,冰冻干燥得目标抗菌肽。
本发明中,刺激诱导黑水虻产生抗菌肽的菌体包括但不限于米曲霉、乳酸杆菌等非致病菌;刺激诱导方式包括但不限于菌剂饲喂、菌悬液浸泡。
采用上述技术方案的本发明,与现有技术相比,有益效果是:
本发明应用低成本黑水虻制备广谱抗菌肽,生产成本低廉,适用于大规模生产;本发明生产的抗菌肽为粗品,可以作为抗菌肽原料药。本发明利用垃圾养殖黑水虻,使垃圾变废为宝,产品价值得到提高。
具体实施方式
以下结合实施例详述本发明。
一种黑水虻抗菌肽的提取方法,按以下步骤进行:
(1)用畜禽粪便、餐饮垃圾、蔬菜水果垃圾、作物秸秆等有机废弃物制备黑水虻养殖料900公斤;
(2)制备米曲霉种子液100公斤,制备米曲霉乳酸杆菌发酵液200公斤;
(3)将米曲霉种子液100公斤加入到900公斤养殖料中制成发酵养殖料1000公斤;
(4)在1000公斤发酵养殖料中投放黑水虻虫卵100克,幼虫达到5龄期,分离虫体和虫砂,得到虫体180公斤;
(5)将180公斤幼虫虫体置于200公斤发酵液中再养殖24小时;
(6)将步骤(5)的180公斤幼虫虫体和200公斤发酵液置于胶体磨,粉碎均质10分钟,置于恒温反应器中,30℃下反应15小时得到降解液;
(7)10000转/分,离心步骤(6)的降解液得降解物的上清液180升;
(8)降解物上清液加入0.5mol/L三氯乙酸,静置30分钟后10000转/分再次离心沉淀大分子蛋白,得去大分子上清液170升;
(9)上清液用海藻酸吸附,过滤,再用0.2mol/L盐酸溶液洗脱吸附在海藻酸上的抗菌肽,得洗脱液40升;
(10)将洗脱液用氢氧化钠调解PH值为4.5后,用氯化钠沉析,透析,脱盐干燥得抗菌肽粗品90克;
(11)将抗菌肽粗品用超纯水溶解,经过0.22μm-0.45μm超滤膜过滤,得到超滤液,冰冻干燥得目标抗菌肽60克。
以上对本发明做了示例性的描述,应该说明的是,在不脱离本发明的核心的情况下,任何简单的变形、修改或者其他本领域技术人员能够不花费创造性劳动的等同替换均落入本发明的保护范围。

Claims (1)

1.一种黑水虻抗菌肽的提取方法,其特征在于,按下述步骤进行:
(1)按米曲霉制种方法制备一定量的米曲霉种子液,米曲霉种子液加入乳酸杆菌扩大培养24小时成为发酵液,发酵液中乳酸杆菌菌体密度2-4亿个/升;
(2)用有机废弃物制备黑水虻养殖料,有机废弃物包括畜禽粪便、餐饮垃圾、蔬菜水果垃圾、作物秸秆;
(3)接种米曲霉种子液于黑水虻养殖料中,米曲霉种子液与黑水虻养殖料的质量比为1:10-20,制备成发酵养殖料;
(4)将黑水虻幼虫投入步骤(3)制备的发酵养殖料中,控制养殖环境温度25-32℃,湿度65-75%,养殖幼虫达到5龄幼虫;
(5)将步骤(4)养殖的黑水虻5龄幼虫与发酵养殖料分离,将5龄幼虫置于步骤(1)制备的发酵液中再养殖24小时,米曲霉和乳酸杆菌诱导黑水虻产生更多抗菌肽;
(6)将步骤(5)养殖24小时的幼虫及发酵液一起置于胶体磨,粉碎均质10-20分钟后置于恒温反应器中,30℃下反应12-36小时得到降解液,米曲霉蛋白酶会将黑水虻部分大分子蛋白降解为小分子的类抗菌肽;
(7)将步骤(6)得到的降解液用10000转/分,离心去除非蛋白杂质,得到降解物的上清液;
(8)降解物上清液以0.5mol/L三氯乙酸沉淀大分子蛋白,静置30分钟后10000转/分再次离心,得到去大分子上清液;
(9)去大分子上清液用海藻酸过滤吸附,再用0.2mol/L盐酸溶液洗脱吸附在海藻酸上的抗菌肽;
(10)将洗脱液用氢氧化钠调解PH值为4.5后,用氯化钠沉析,透析,脱盐干燥得抗菌肽粗品;
(11)将抗菌肽粗品用超纯水溶解,经过0.22μm-0.45μm超滤膜过滤,得到超滤液,冰冻干燥得目标抗菌肽。
CN202010708205.0A 2020-07-22 2020-07-22 一种黑水虻抗菌肽的提取方法 Pending CN111876456A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010708205.0A CN111876456A (zh) 2020-07-22 2020-07-22 一种黑水虻抗菌肽的提取方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010708205.0A CN111876456A (zh) 2020-07-22 2020-07-22 一种黑水虻抗菌肽的提取方法

Publications (1)

Publication Number Publication Date
CN111876456A true CN111876456A (zh) 2020-11-03

Family

ID=73155986

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010708205.0A Pending CN111876456A (zh) 2020-07-22 2020-07-22 一种黑水虻抗菌肽的提取方法

Country Status (1)

Country Link
CN (1) CN111876456A (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112321697A (zh) * 2020-12-15 2021-02-05 天津市农业科学院 一种黑水虻抗菌肽Cecropin-α及其应用
CN113548703A (zh) * 2021-07-22 2021-10-26 浙江智飨科技有限公司 智慧绿色环境数字化产业管理平台

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0182278A2 (en) * 1984-11-19 1986-05-28 Wakunaga Seiyaku Kabushiki Kaisha Antibacterial polypeptide, preparation thereof, and use thereof
KR19990070300A (ko) * 1998-02-18 1999-09-15 변재형 아스퍼질러스 오리재 pf 및 이로부터 생산된단백질 분해효소
RU2552157C1 (ru) * 2013-12-26 2015-06-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Санкт-Петербургский государственный университет" (СПбГУ) Способ получения комплекса антимикробных пептидов насекомого
WO2015087919A1 (ja) * 2013-12-10 2015-06-18 株式会社明治 抗菌ペプチド誘導剤
CN104844288A (zh) * 2015-06-15 2015-08-19 重庆光耀农业科技发展有限公司 一种利用黑水虻处理牛粪制备有机肥的方法
CN105598121A (zh) * 2014-11-24 2016-05-25 深圳市腾浪再生资源发展有限公司 一种餐厨垃圾生物处理的工艺
KR20180035013A (ko) * 2016-09-28 2018-04-05 동국대학교 경주캠퍼스 산학협력단 단백질 가수분해효소 저항성을 가지는 동애등에 유충 항균 추출물을 포함하는 조성물의 제조방법
CN108372187A (zh) * 2018-05-07 2018-08-07 西安洁姆环保科技有限责任公司 餐厨废弃物资源化利用及无公害处理系统与方法
CN111334547A (zh) * 2020-02-15 2020-06-26 松颉环保科技(深圳)有限公司 一种黑水虻抗菌肽的提取方法及其在制备抗烟草病害的肥料中的应用

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0182278A2 (en) * 1984-11-19 1986-05-28 Wakunaga Seiyaku Kabushiki Kaisha Antibacterial polypeptide, preparation thereof, and use thereof
KR19990070300A (ko) * 1998-02-18 1999-09-15 변재형 아스퍼질러스 오리재 pf 및 이로부터 생산된단백질 분해효소
WO2015087919A1 (ja) * 2013-12-10 2015-06-18 株式会社明治 抗菌ペプチド誘導剤
RU2552157C1 (ru) * 2013-12-26 2015-06-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Санкт-Петербургский государственный университет" (СПбГУ) Способ получения комплекса антимикробных пептидов насекомого
CN105598121A (zh) * 2014-11-24 2016-05-25 深圳市腾浪再生资源发展有限公司 一种餐厨垃圾生物处理的工艺
CN104844288A (zh) * 2015-06-15 2015-08-19 重庆光耀农业科技发展有限公司 一种利用黑水虻处理牛粪制备有机肥的方法
KR20180035013A (ko) * 2016-09-28 2018-04-05 동국대학교 경주캠퍼스 산학협력단 단백질 가수분해효소 저항성을 가지는 동애등에 유충 항균 추출물을 포함하는 조성물의 제조방법
CN108372187A (zh) * 2018-05-07 2018-08-07 西安洁姆环保科技有限责任公司 餐厨废弃物资源化利用及无公害处理系统与方法
CN111334547A (zh) * 2020-02-15 2020-06-26 松颉环保科技(深圳)有限公司 一种黑水虻抗菌肽的提取方法及其在制备抗烟草病害的肥料中的应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HEIKO VOGEL: "Nutritional immunology: Diversification and diet-dependent expression of antimicrobial peptides in the black soldier fly Hermetia illucens", 《DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY》, vol. 78, pages 141 - 148 *
张俊哲: "黑水虻的养殖及其处理畜禽粪便的研究进展", 《山东畜牧兽医》, vol. 40, no. 1, pages 270 - 271 *
李冬宝: "胡文峰:利用微生物及黑水虻处理畜禽粪便可不断提高其利用利润", 《北方牧业》, vol. 20, pages 23 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112321697A (zh) * 2020-12-15 2021-02-05 天津市农业科学院 一种黑水虻抗菌肽Cecropin-α及其应用
CN112321697B (zh) * 2020-12-15 2022-01-21 天津市农业科学院 一种黑水虻抗菌肽Cecropin-α及其应用
CN113548703A (zh) * 2021-07-22 2021-10-26 浙江智飨科技有限公司 智慧绿色环境数字化产业管理平台
CN113548703B (zh) * 2021-07-22 2022-02-11 浙江智飨科技有限公司 智慧绿色环境数字化产业管理平台

Similar Documents

Publication Publication Date Title
CN107245457A (zh) 一种铁皮石斛内生真菌菌株及其产生的胞外多糖以及该胞外多糖的提取方法与应用
CN101717744B (zh) 一种基因重组解淀粉芽孢杆菌及菌剂制备方法
CN101974586A (zh) 一种枯草芽孢杆菌抗菌脂肽的生产方法及其在饲料中的应用
CN1840683A (zh) 利用淀粉液化芽孢杆菌生产抗菌肽的方法及产品
CN108048373B (zh) 一株枯草芽孢杆菌ah1005及其应用
CN111876456A (zh) 一种黑水虻抗菌肽的提取方法
CN102524518A (zh) 一种利用侧芽孢杆菌生产抗菌肽的方法
CN105175518A (zh) 凝结芽孢杆菌fm603产生的细菌素及其制备方法
CN104388341B (zh) 一种胶质芽孢杆菌菌株及其在海藻降解中的应用
KR101311192B1 (ko) 꿀벌의 전염병 예방 및 치료 조성물의 제조방법 및 이 방법에 의한 꿀벌용 영양조성물
CN111134231B (zh) 一株漳州芽孢杆菌及利用其发酵桑叶粉的方法
CN104560855B (zh) 缺失phoP的鸭疫里默氏杆菌CH1减毒菌株及构建方法
CN103266074B (zh) 一种枯草芽孢杆菌菌株及其应用
KR101986746B1 (ko) 라이조푸스 속 균주와 키토산을 포함하는 액상 비료 조성물 및 이의 제조 방법
CN114058661A (zh) 抗革兰氏阳性菌的生物活性肽制剂的产业化制备方法
CN105907674A (zh) 一种复合微生态制剂的制备方法及其应用
CN111996139B (zh) 一种提高肉犊牛生产效益的复合微生态制剂及应用
CN113046085A (zh) 一种用于盐碱地改良的动物酵素及其制备方法
CN101003789B (zh) 蜘蛛毒肽基因重组的工程菌菌剂
CN107937301A (zh) 一种解淀粉芽孢杆菌及其在水产养殖中的应用
CN111733117B (zh) 一株产抗菌肽的海洋芽孢杆菌及其发酵方法和应用
CN109943507B (zh) 一种兽用a型产气荚膜梭菌类毒素的制备方法及其应用
CN107502603A (zh) 一种大肠杆菌裂解酶及其制备方法与应用
CN106086120A (zh) 一种链霉菌发酵培养基
CN106086122A (zh) 一种发酵制备链霉素的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination