CN111771628A - Method for rapidly screening vitex rotundifolia with high salt tolerance - Google Patents
Method for rapidly screening vitex rotundifolia with high salt tolerance Download PDFInfo
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- CN111771628A CN111771628A CN202010599751.5A CN202010599751A CN111771628A CN 111771628 A CN111771628 A CN 111771628A CN 202010599751 A CN202010599751 A CN 202010599751A CN 111771628 A CN111771628 A CN 111771628A
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- salt tolerance
- vitex rotundifolia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
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- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for rapidly screening vitex rotundifolia with high salt tolerance, which comprises the following steps: planting single-leaf vitex rotundifolia seeds in a single-leaf vitex rotundifolia test field; irrigating with water solutions with different salt concentrations and managing uniformly; cutting 2-year-old branches as cuttings, soaking the cuttings in a rooting solution for 1-2 hours, cutting the cuttings into plain sand, irrigating aqueous solutions with different salt concentrations, and finally judging the salt tolerance according to the survival rate of the cuttings with different planting resources in the plain sand with different salt concentrations; and comparing whether the potted plant salt tolerance test result is consistent with the cutting salt tolerance test result. The invention establishes a method for rapidly screening the single-leaf vitex rotundifolia planting resources with high salt tolerance degree by utilizing the asexual propagation capacity of the single-leaf vitex rotundifolia and comparing the two test results of a potted plant salt tolerance test and a cutting salt tolerance test, and has the advantages of simple screening technology, reduced workload, shortened screening period and accelerated screening process.
Description
Technical Field
The invention relates to the technical field of vitex rotundifolia salt tolerance degree identification, in particular to a method for rapidly screening vitex rotundifolia with high salt tolerance degree.
Background
The vitex rotundifolia is an excellent ground cover plant, has the characteristics of quick growth, strong stress resistance and easy propagation, has strong wind resistance, drought resistance and saline-alkali resistance, and is particularly suitable for growing in sandy soil and alkaline soil areas. The single-leaf vitex rotundifolia planted in the saline-alkali soil can increase the ground coverage, reduce the soil evaporation, absorb and take away the salt in the soil, thereby reducing the accumulation of the salt in the plough layer. In addition, the vitex rotundifolia also has the effect of improving saline-alkali soil, such as increasing the organic matter content of soil, improving the soil structure, reducing the soil salinity, increasing the porosity of the soil, improving the biological activity of the soil and the like.
By utilizing the characteristics of the vitex rotundifolia, the vitex rotundifolia can be planted on the coastal beach, so that the ornamental purpose can be achieved, the coastal saline-alkali soil can be improved, the wind prevention and sand fixation effects can be achieved, and the vitex rotundifolia can be planted on the saline-alkali soil to achieve the effects of reducing salt and improving soil. Therefore, the saline-alkali soil is scientifically and reasonably treated by using biological improvement measures and taking the saline-alkali soil as an available resource, and the saline-alkali soil is biologically repaired by using the halophyte vitex rotundifolia, so that the saline-alkali soil is optimized and improved by abundant halophyte resources on the earth, and the method has great significance.
The final aim of researching the salt tolerance physiology of plants is to clarify the salt tolerance mechanism of plants and culture resistant varieties. Although the salt tolerance of plants has been studied in a great deal and reaches a certain extent and depth, the salt tolerance of plants is a quantitative character controlled by multiple genes and influenced by the species, the genotype, the morphological character and the internal physiological and biochemical reactions of the plants, which determines the complexity of the salt tolerance of the plants. With the development of molecular biology research technology and genetic engineering technology, people begin to know the mechanism of plant salt tolerance at molecular level such as gene composition, expression regulation and signal conduction. After a lot of research, people have isolated some related salt-induced genes by using a genomics method in recent years, and have studied the functions of the genes in detail. By means of gene engineering, the salt-resistant exogenous target gene is introduced into plant by means of DNA recombination and transgenic technology, and developed into a new way of improving the salt stress resistance of plant. However, at present, the salt tolerance research on the vitex rotundifolia is still in a preliminary research stage, and the preliminary knowledge exploration for understanding the research on physiological and biochemical aspects of the vitex rotundifolia is not enough to quickly identify and screen the vitex rotundifolia with high salt tolerance.
Disclosure of Invention
In order to solve the technical problem, the invention discloses a method for rapidly screening vitex rotundifolia with high salt tolerance, which comprises the following steps:
(1) planting plants: planting the collected vitex rotundifolia seeds in a vitex rotundifolia test field after treatment;
(2) potted plant salt tolerance test: irrigating with water solutions with different salt concentrations and managing uniformly, and finally judging the salt tolerance degree through the growth amount of the vitex rotundifolia;
(3) salt resistance test of cutting slips: cutting 2-year-old branches as cuttings, soaking the cuttings in a rooting solution for 1-2 hours, cutting the cuttings into plain sand, irrigating aqueous solutions with different salt concentrations, and finally judging the salt tolerance according to the survival rate of the cuttings with different planting resources in the plain sand with different salt concentrations;
(4) the results of the two tests were analyzed together: comparing whether the potted plant salt tolerance test result is consistent with the cutting salt tolerance test result; if the salt tolerance is consistent, the salt tolerance test of the cuttings can be a method for judging the salt tolerance degree of the vitex rotundifolia.
Furthermore, 2 tons/mu of organic fertilizer is spread in the test field in the step (1), rotary tillage is carried out on the soil, the sowing depth is 3-5cm, the sowing distance is 1m, and watering is carried out in time after sowing.
Further, the aqueous solutions with different salt concentrations in the step (2) are as follows: the salt concentration of the aqueous solution prepared by sodium chloride is 0.3 to 1.2 percent.
Further, the soaked branches are taken out and dried in the step (3), and are inserted into the plain sand according to the plant row spacing of 7 x 10 cm.
The invention has the beneficial effects that:
1. the invention utilizes the asexual propagation capacity of the vitex rotundifolia, and establishes a method for rapidly screening the vitex rotundifolia planting resources with high salt tolerance degree by comparing two test results of a pot salt tolerance test and a cutting salt tolerance test;
2. the method for screening the high-salt-tolerance planting resources through the cutting salt-tolerance test has the advantages of simple screening technology, reduced workload, shortened screening period and accelerated screening process;
3. the salt concentration of the aqueous solution in the salt tolerance test of the cuttings can provide data support for screening planting resources with high salt tolerance of the vitex rotundifolia;
4. the high-salt-tolerance planting resource bred by the method has important significance for accelerating the screening of the salt and alkali-tolerant variety of the vitex rotundifolia, increasing the selection of green seedlings in the saline-alkali soil and the like.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The first embodiment is as follows:
1. collecting and planting single-leaf vitex rotundifolia planting resources
1.1 Collection of Simpleleaf Shrub Chastetree seeds
And collecting and screening mature and full single-leaf vitex rotundifolia seeds without diseases and insect pests in a single-leaf vitex rotundifolia planting area.
1.2 planting of Simpleleaf chastetree
1.2.1 land preparation
And (3) broadcasting 2 tons/mu of organic fertilizer on the saline-alkali soil test field, and carrying out rotary tillage on the soil.
1.2.2 sowing
Sowing at the bottom of 3 months, wherein the sowing depth is 3-5cm, the sowing distance is 1m, and watering is carried out in time after the sowing is finished.
1.2.3 daily management
Timely weeding, watering and other works are carried out according to the actual conditions in the field.
2. Detection of survival rate of cuttings of different single-leaf vitex planting resources
2.1 Single-leaf Vitex trifolia shoot treatment
The branches of 2-year-old single-leaf chaste tree branches are cut by scissors in month 5 to be about 20cm, the upper parts of the branches are cut to be flat, 2-3 leaves are left, and the lower parts of the branches are cut to be oblique.
2.2 rooting solution treatment
Placing the treated branches into a rooting solution for about 1-2h, taking out, airing, and inserting into plain sand according to the plant row spacing of 7 x 10 cm; wherein 100 single-leaf vitex rotundifolia cutting shoots are used for each variety.
2.3 survival Rate statistics
Inserting the cutting slips into plain sand, thoroughly watering with water, building a shade shed, and watering according to conditions; the statistics of the survival condition of the cutting in 10 months are as follows:
TABLE 1 statistics table for survival rate of different varieties of vitex rotundifolia
| Numbering | Survival rate | Numbering | Survival rate | Numbering | Survival rate |
| D-001 | 97％ | D-005 | 98％ | D-009 | 96％ |
| D-002 | 96％ | D-006 | 97％ | D-010 | 98％ |
| D-003 | 98％ | D-007 | 97％ | D-011 | 97％ |
| D-004 | 97％ | D-008 | 98％ | D-012 | 96％ |
The survival rate difference among different varieties can be seen through the survival rate of the cuttings of the germplasm resources of 12 different single-leaf vitex rotundifolia.
3. Salt tolerance test of potted plants
3.1 Single leaf Vitex rotundifolia transplantation
Transplanting the survival vitex rotundifolia into a test pot and managing.
3.2 preparation of aqueous solutions of different salt concentrations
Sodium chloride is used to prepare aqueous solution with salt concentration of 0.3%, 0.6%, 0.9% and 1.2% for standby.
3.3 salt tolerance test
After the potted single-leaf vitex rotundifolia is transplanted to survive, selecting single-leaf vitex rotundifolia with the same growth vigor, wherein each variety has 20 pots; the prepared aqueous sodium chloride solution was poured into each pot, and 600ml of water was added to the control, and the solution was added every 10 days.
3.4 statistics of results
After 4 months, the increase in weight of the vitex rotundifolia plants compared to the control was determined as follows:
4. salt tolerance test of cutting
4.1 Single-leaf Vitex trifolia shoot treatment
Using scissors to cut 2-year-old single-leaf shrub chastetree branches about 20cm, cutting the upper part of the branches to be flat and leaving 2-3 leaves, and cutting the lower part of the branches to be oblique.
4.2 rooting solution treatment
Placing the treated branches into a rooting solution for about 1 hour, taking out, airing, and inserting into plain sand according to the plant row spacing of 7 x 10 cm; wherein 100 single-leaf vitex rotundifolia cutting shoots are used for each variety.
4.3 preparation of aqueous solutions of different salt concentrations
Sodium chloride is used to prepare aqueous solution with salt concentration of 0.3%, 0.6%, 0.9% and 1.2% for standby.
4.4 salt tolerance test of cuttings
Cutting the cutting slips in plain sand and managing according to the same method, after the cutting slips root, adding 600ml of water into each pot, adding 600ml of water in contrast, adding the water solution once every 10 days, adding 3 times of sodium chloride water solution in total, and managing according to the same method.
4.5 statistics of results
The survival rate of the single-leaf vitex rotundifolia cutting shoots is calculated, and the result is as follows:
5. comprehensive analysis of two test results
As can be seen by comparing the data of the cuttings salt-tolerance test and the potted plant salt-tolerance test, the salt-tolerance results of different varieties of the vitex rotundifolia are basically consistent in the two different tests, wherein the salt tolerance degrees of the vitex rotundifolia are D-002, D-005, D-009 and D-0012 which are numbered as high as D-002, D-005, D-009 and D-0012, and the salt tolerance degrees of the D-003, D-004, D-007 and D-008 are low.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein. In addition, the technical solutions between the various embodiments can be combined with each other, but must be based on the realization of those skilled in the art; where combinations of features are mutually inconsistent or impractical, such combinations should not be considered as being absent and not within the scope of the claimed invention.
Claims (4)
1. A method for rapidly screening vitex rotundifolia with high salt tolerance is characterized by comprising the following steps:
(1) planting plants: planting the collected vitex rotundifolia seeds in a vitex rotundifolia test field after treatment;
(2) potted plant salt tolerance test: irrigating with water solutions with different salt concentrations and managing uniformly, and finally judging the salt tolerance degree through the growth amount of the vitex rotundifolia;
(3) salt resistance test of cutting slips: cutting 2-year-old branches as cuttings, soaking the cuttings in a rooting solution for 1-2 hours, cutting the cuttings into plain sand, irrigating aqueous solutions with different salt concentrations, and finally judging the salt tolerance according to the survival rate of the cuttings with different planting resources in the plain sand with different salt concentrations;
(4) the results of the two tests were analyzed together: comparing whether the potted plant salt tolerance test result is consistent with the cutting salt tolerance test result; if the salt tolerance is consistent, the salt tolerance test of the cuttings can be a method for judging the salt tolerance degree of the vitex rotundifolia.
2. The method for rapidly screening the vitex rotundifolia with high salt tolerance according to claim 1, which is characterized in that: in the step (1), 2 tons/mu of organic fertilizer is spread in the test field, rotary tillage is carried out on the test field, the sowing depth is 3-5cm, the sowing distance is 1m, and watering is carried out in time after the sowing is finished.
3. The method for rapidly screening the vitex rotundifolia with high salt tolerance according to claim 1, which is characterized in that: the water solutions with different salt concentrations in the step (2) are as follows: the salt concentration of the aqueous solution prepared by sodium chloride is 0.3 to 1.2 percent.
4. The method for rapidly screening the vitex rotundifolia with high salt tolerance according to claim 1, which is characterized in that: and (3) taking out the soaked branches, airing the branches, and inserting the branches into plain sand according to the plant row spacing of 7 x 10 cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114375751A (en) * | 2021-12-28 | 2022-04-22 | 广州华苑园林股份有限公司 | Coastal saline-alkali site restoration method and application thereof |
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2020
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114375751A (en) * | 2021-12-28 | 2022-04-22 | 广州华苑园林股份有限公司 | Coastal saline-alkali site restoration method and application thereof |
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Application publication date: 20201016 |