CN111771615A - Three-dimensional soilless organic cultivation method for lucid ganoderma - Google Patents
Three-dimensional soilless organic cultivation method for lucid ganoderma Download PDFInfo
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- CN111771615A CN111771615A CN202010828432.7A CN202010828432A CN111771615A CN 111771615 A CN111771615 A CN 111771615A CN 202010828432 A CN202010828432 A CN 202010828432A CN 111771615 A CN111771615 A CN 111771615A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a three-dimensional soilless organic cultivation method of lucid ganoderma, which comprises the following steps: mixing paper mulberry branches, sweet potato vines, rotten wood, sweet potato powder and bagasse, crushing, sieving, adding water in the stirring process, naturally fermenting, adding tea powder, gypsum powder, lime powder, calcium carbonate, glucose and magnesium sulfate, and uniformly mixing to obtain a cultivation material; putting the cultivation material into a fungus bag, sterilizing at high pressure, naturally cooling to room temperature, and taking out of the pot to obtain a ganoderma lucidum bag material; controlling the temperature to be 24-26 ℃ in the first 10-12 days after the ganoderma lucidum bag material is inoculated; then adjusting the temperature to 21-23 ℃, maintaining the temperature at 21-23 ℃ for 15-20 days, and then adjusting the temperature to 20-21 ℃; removing the fungus bag when the hypha grows to 14-16cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation fungus rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 80-88%, and harvesting after the pileus grows until the white point disappears.
Description
Technical Field
The invention relates to the technical field of lucid ganoderma, in particular to a three-dimensional soilless organic cultivation method of lucid ganoderma.
Background
The shape of the ganoderma lucidum is umbrella-shaped, the shape of the umbrella kidney, semicircle or nearly round, and is the fruiting body of the fungus ganoderma lucidum of the polyporaceae, the modern medicine proves that the ganoderma lucidum contains various physiological active substances, can adjust and enhance the immunity of human bodies, and has good synergistic treatment effect on neurasthenia, rheumatoid arthritis, coronary heart disease, hypertension, hepatitis, grape diabetes, tumors and the like, so the cultivation of the ganoderma lucidum becomes one of the main agricultural practices of farmers in mountainous areas of China.
At present, ganoderma lucidum in mountainous areas almost always adopts mushroom sticks to cultivate in a greenhouse built outdoors, needs rich and complete nutrient substances in the cultivation process, but still has the problems of poor water and fertilizer retention and difficulty in fixing plant roots.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a three-dimensional soilless organic cultivation method for lucid ganoderma.
A three-dimensional soilless organic cultivation method of lucid ganoderma comprises the following steps:
s1, mixing the paper mulberry branches, the sweet potato vines, the rotten wood, the sweet potato powder and the bagasse, crushing, sieving to obtain particles with the particle size of 2-3mm, adding water in the stirring process until the water content is 25-35 wt%, naturally fermenting, adding the tea powder, the gypsum powder, the lime powder, the calcium carbonate, the glucose and the magnesium sulfate, and uniformly mixing to obtain the cultivation material;
s2, filling the cultivation material into fungus bags, wherein no gap exists between the cultivation material and the fungus bags, tightly binding the mouths of the fungus bags, putting the fungus bags into a steamer, sterilizing the fungus bags with high-pressure steam, naturally cooling the fungus bags to room temperature, and taking the fungus bags out of the steamer to obtain ganoderma lucidum bag materials;
s3, after inoculating the lucid ganoderma bag material, controlling the temperature to be 24-26 ℃ in the first 10-12 days; then adjusting the temperature to 21-23 ℃, maintaining the temperature at 21-23 ℃ for 15-20 days, and then adjusting the temperature to 20-21 ℃;
removing the fungus bag when the hypha grows to 14-16cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation fungus rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 80-88%, and harvesting after the pileus grows until the white point disappears.
Preferably, in S1, the mass ratio of the branches of paper mulberry, the sweet potato vine, the rotten wood, the sweet potato powder, the bagasse, the tea powder, the gypsum powder, the lime powder, the calcium carbonate, the glucose and the magnesium sulfate is 100: 10-20: 5-12: 5-10: 1-5: 2-6: 0.5-1.2: 0.5-1.5: 1-2: 1-2: 0.1-0.6.
Preferably, in S1, the natural fermentation time is 10-16 days, the pile height in the fermentation process is 1.5-2m, the pile width is 2.5-3m, and the pile length is 3.2-3.6 m.
Preferably, in S1, the water content is maintained at 25 wt% or more during the fermentation, and the stack is turned when the temperature is higher than 55 ℃.
Preferably, in S2, a high-pressure polyethylene bag tube with a folding width of 12-14cm, a length of 10-13cm and a thickness of 0.002-0.005cm is used as the fungus bag.
Preferably, in S3, the nutrient medium coverage is 1.5-2.5cm thick.
Preferably, in the preparation process of the nutrient medium of S3, uniformly mixing the cottonseed hulls, the waste coal cinder and the dry pigeon manure, adding water until the water content is 30-40 wt%, sending into a high-pressure steam explosion device, wherein the explosion pressure is 7.2-8MPa, adding a fermentation microbial inoculum for fermentation until the pH value of the system is 7.1-7.4, adding bentonite particles, and uniformly stirring to obtain the nutrient medium.
Preferably, the mass ratio of the cottonseed hulls, the waste coal cinder, the dry pigeon manure, the fermentation bacteria agent and the bentonite particles is 30-40: 10-18: 2-6: 0.1-0.15: 30-70.
Preferably, the concentration of the bacillus subtilis in the fermentation inoculum is 1-1.5 × 109cfu/g, the concentration of the bacillus licheniformis is 1.3-2.2 × 109cfu/g。
The technical effects of the invention are as follows:
the invention adopts cottonseed hulls and waste coal cinder as main materials, and the cottonseed hulls and the waste coal cinder are matched with dry pigeon manure for steam explosion and then fermentation, the obtained nutrient medium not only contains rich organic matters, but also has rich nutrient substances and a certain slow release effect, and the nutrient medium is moved out of a sesame shed after hyphae are fully distributed, covered by the nutrient medium, has loose texture and proper weight, has good air permeability and can effectively fix the root system of the ganoderma lucidum;
the ganoderma lucidum bag material takes paper mulberry branches, sweet potato vines, rotten wood, sweet potato powder and bagasse as main materials, and is naturally fermented in a stacking mode, wherein lignin, cellulose, hemicellulose, pectin and other substances are decomposed to form simple substances beneficial to absorption of ganoderma lucidum, rapid growth of ganoderma lucidum mycelia is promoted, the ganoderma lucidum bag material is further matched with tea powder, gypsum powder, lime powder, calcium carbonate, glucose and magnesium sulfate to act, ingredients are reasonable, nutrition is uniform, and the ganoderma lucidum bag material is good in growth vigor and high in yield after mycelia are produced;
the invention adopts a three-dimensional soilless culture method for the lucid ganoderma, can realize standardized culture, effectively utilizes land resources, saves manpower and material resources, can change the unicity of the conventional culture material by the matching action of the lucid ganoderma bag material and the nutrient substrate, achieves the complementary action of nutrient substances, is more suitable for the rapid growth and development of the lucid ganoderma, and has the advantages that the lucid ganoderma growing time is advanced by more than 8 days compared with the conventional culture method when the bag is full of hyphae, the hyphae are thick and strong in growth performance, and the lucid ganoderma is covered with large and thick flesh.
Detailed Description
The technical solution of the present invention will be described in detail below with reference to specific examples.
Example 1
A three-dimensional soilless organic cultivation method of lucid ganoderma comprises the following steps:
s1, mixing 100kg of paper mulberry branches, 10kg of sweet potato vines, 12kg of rotten wood, 5kg of sweet potato powder and 5kg of bagasse, crushing and sieving to obtain particles with the particle size of 2mm, adding water in the stirring process until the water content is 35 wt%, naturally fermenting for 10 days, wherein the pile height is 2m, the pile width is 2.5m, and the pile length is 3.6m in the fermentation process, keeping the water content at 25 wt% in the fermentation process, turning over the pile when the temperature is higher than 55 ℃, adding 6kg of tea powder, 0.5kg of gypsum powder, 1.5kg of lime powder, 1kg of calcium carbonate, 2kg of glucose and 0.1kg of magnesium sulfate, and uniformly mixing to obtain a cultivation material;
s2, adopting a high-pressure polyethylene bag cylinder with a folding width of 14cm, a length of 10cm and a thickness of 0.005cm as a fungus bag, putting the cultivation material into the fungus bag, enabling no gap to be formed between the cultivation material and the wall of the fungus bag, tightening the opening of the fungus bag, then sterilizing for 1 hour by high-pressure steam, enabling the sterilization pressure to be 1.4MPa, naturally cooling to room temperature, taking out of the pot, and obtaining the ganoderma lucidum bag material;
s3, drying 30kg of cottonseed hulls, 18kg of waste coal cinder and 2kg of waste coal cinderUniformly mixing pigeon dung, adding water until the water content is 40 wt%, feeding into a high-pressure steam explosion device, wherein the explosion pressure is 7.2MPa, adding 0.15kg of fermentation inoculum, fermenting until the pH value of the system is 7.1-7.4, adding 30kg of bentonite particles, and uniformly stirring to obtain a nutrient medium, wherein the concentration of bacillus subtilis in the fermentation inoculum is 1.5 × 109cfu/g, the concentration of the bacillus licheniformis is 1.3 × 109cfu/g；
Controlling the temperature to be 24 ℃ in the first 10-12 days after the ganoderma lucidum bag material is inoculated; then adjusting the temperature to 23 ℃, maintaining the temperature at 23 ℃ for 15-20 days, and then adjusting the temperature to 21 ℃;
removing the fungus bag when the hyphae grow to 16cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation mushroom rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 80%, and harvesting after mushroom caps grow until white points disappear.
Example 2
A three-dimensional soilless organic cultivation method of lucid ganoderma comprises the following steps:
s1, mixing 100kg of paper mulberry branches, 20kg of sweet potato vines, 5kg of rotten wood, 10kg of sweet potato powder and 1kg of bagasse, crushing and sieving to obtain particles with the particle size of 3mm, adding water in the stirring process until the water content is 25 wt%, naturally fermenting for 16 days, wherein the pile height is 1.5m, the pile width is 3m, and the pile length is 3.2m in the fermentation process, keeping the water content at 35 wt% in the fermentation process, turning over the pile when the temperature is higher than 55 ℃, adding 1.2kg of gypsum powder, 0.5kg of lime powder, 2kg of calcium carbonate, 1kg of glucose and 0.6kg of magnesium sulfate, and uniformly mixing to obtain a cultivation material;
s2, adopting a high-pressure polyethylene bag cylinder with a folding width of 12cm, a length of 13cm and a thickness of 0.002cm as a fungus bag, putting the cultivation material into the fungus bag, enabling no gap to be formed between the cultivation material and the wall of the fungus bag, tightening the opening of the fungus bag, then sterilizing for 2 hours by high-pressure steam, enabling the sterilization pressure to be 1.1MPa, naturally cooling to room temperature, taking out of the pot, and obtaining the ganoderma lucidum bag material;
s3, uniformly mixing 40kg of cottonseed hulls, 10kg of waste coal cinder and 6kg of dry pigeon manure, adding water until the water content is 30 wt%, sending the mixture into a high-pressure steam explosion device, wherein the explosion pressure is 8MPa, adding 0.1kg of fermentation inoculant to ferment until the pH value of the system is 7.1-7.4, and adding 70kg of expansion inoculantThe soil moistening particles are evenly stirred to obtain a nutrient substrate, and the concentration of the bacillus subtilis in the fermentation inoculum is 1 × 109cfu/g, the concentration of the bacillus licheniformis is 2.2 × 109cfu/g；
Controlling the temperature to be 26 ℃ in the first 10-12 days after the ganoderma lucidum bag material is inoculated; then adjusting the temperature to 21 ℃, maintaining the temperature at 21 ℃ for 15-20 days, and then adjusting the temperature to 20 ℃;
removing the fungus bag when the hyphae grow to 14cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation mushroom rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 88%, and harvesting after mushroom caps grow until white points disappear.
Example 3
A three-dimensional soilless organic cultivation method of lucid ganoderma comprises the following steps:
s1, mixing 100kg of paper mulberry branches, 17kg of sweet potato vines, 8kg of rotten wood, 8kg of sweet potato powder and 2kg of bagasse, crushing and sieving to obtain particles with the particle size of 2.8mm, adding water in the stirring process until the water content is 28 wt%, naturally fermenting for 14 days, wherein the pile height is 1.6m, the pile width is 2.8m, and the pile length is 3.3m in the fermentation process, keeping the water content at 32 wt% in the fermentation process, turning over when the temperature is higher than 55 ℃, adding 3kg of tea powder, 1kg of gypsum powder, 0.8kg of lime powder, 1.7kg of calcium carbonate, 1.2kg of glucose and 0.5kg of magnesium sulfate, and uniformly mixing to obtain the cultivation material;
s2, using a high-pressure polyethylene bag cylinder with a folding width of 12.5cm, a length of 12cm and a thickness of 0.003cm as a fungus bag, putting the cultivation material into the fungus bag, wherein no gap exists between the cultivation material and the wall of the fungus bag, performing high-pressure steam sterilization for 1.7 hours after tightening the opening of the fungus bag, wherein the sterilization pressure is 1.2MPa, naturally cooling to room temperature, and taking out of the pot to obtain the ganoderma lucidum bag material;
s3, uniformly mixing 37kg of cottonseed hulls, 12kg of waste coal cinder and 5kg of dry pigeon manure, adding water until the water content is 33 wt%, sending the mixture into a high-pressure steam explosion device, wherein the explosion pressure is 7.8MPa, adding 0.12kg of fermentation inoculum, fermenting until the pH value of the system is 7.1-7.4, adding 60kg of bentonite particles, and uniformly stirring to obtain a nutrient substrate, wherein the concentration of bacillus subtilis in the fermentation inoculum is 1.2 × 109cfu/g, the concentration of the bacillus licheniformis is 2 × 109cfu/g；
Controlling the temperature to be 24.5 ℃ in the first 10-12 days after the ganoderma lucidum bag material is inoculated; then adjusting the temperature to 22.5 ℃, maintaining the temperature at 22.5 ℃ for 16-18 days, and then adjusting the temperature to 20.8 ℃;
removing the fungus bag when the hypha grows to 14.5cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation mushroom rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 86%, and harvesting after mushroom caps grow until white points disappear.
Example 4
A three-dimensional soilless organic cultivation method of lucid ganoderma comprises the following steps:
s1, mixing 100kg of paper mulberry branches, 13kg of sweet potato vines, 10kg of rotten wood, 6kg of sweet potato powder and 4kg of bagasse, crushing and sieving to obtain particles with the particle size of 2.2mm, adding water in the stirring process until the water content is 32 wt%, naturally fermenting for 12 days, wherein the pile height is 1.8m, the pile width is 2.6m, and the pile length is 3.5m in the fermentation process, keeping the water content at 28 wt% in the fermentation process, turning over when the temperature is higher than 55 ℃, adding 5kg of tea powder, 0.6kg of gypsum powder, 1.2kg of lime powder, 1.3kg of calcium carbonate, 1.8kg of glucose and 0.2kg of magnesium sulfate, and uniformly mixing to obtain the cultivation material;
s2, adopting a high-pressure polyethylene bag cylinder with a folding width of 13.5cm, a length of 11cm and a thickness of 0.004cm as a fungus bag, putting the cultivation material into the fungus bag, enabling no gap to be formed between the cultivation material and the wall of the fungus bag, performing high-pressure steam sterilization for 1.3 hours after tightening the opening of the fungus bag, enabling the sterilization pressure to be 1.3MPa, naturally cooling to room temperature, taking out of the pot, and obtaining the ganoderma lucidum bag material;
s3, uniformly mixing 33kg of cottonseed hulls, 16kg of waste coal cinder and 3kg of dry pigeon manure, adding water until the water content is 37 wt%, sending the mixture into a high-pressure steam explosion device, wherein the explosion pressure is 7.4MPa, adding 0.14kg of fermentation inoculum, fermenting until the pH value of the system is 7.1-7.4, adding 40kg of bentonite particles, and uniformly stirring to obtain a nutrient substrate, wherein the concentration of bacillus subtilis in the fermentation inoculum is 1.4 × 109cfu/g, the concentration of the bacillus licheniformis is 1.6 × 109cfu/g；
Controlling the temperature to be 25.5 ℃ in the first 10-12 days after the ganoderma lucidum bag material is inoculated; then adjusting the temperature to 21.5 ℃, maintaining the temperature at 21.5 ℃ for 16-18 days, and then adjusting the temperature to 20.2 ℃;
removing the fungus bag when the hypha grows to 15.5cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation fungus rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 82%, and harvesting after the pileus grows until the white point disappears.
Example 5
A three-dimensional soilless organic cultivation method of lucid ganoderma comprises the following steps:
s1, mixing 100kg of paper mulberry branches, 15kg of sweet potato vines, 9kg of rotten wood, 7kg of sweet potato powder and 3kg of bagasse, crushing and sieving to obtain particles with the particle size of 2.5mm, adding water in the stirring process until the water content is 30 wt%, naturally fermenting for 13 days, wherein the pile height is 1.7m, the pile width is 2.7m, the pile length is 3.4m in the fermentation process, keeping the water content at 30 wt% in the fermentation process, turning the pile when the temperature is higher than 55 ℃, adding 4kg of tea powder, 0.8kg of gypsum powder, 1kg of lime powder, 1.5kg of calcium carbonate, 1.5kg of glucose and 0.35kg of magnesium sulfate, and uniformly mixing to obtain the cultivation material;
s2, adopting a high-pressure polyethylene bag cylinder with a folding width of 13cm, a length of 11.5cm and a thickness of 0.0035cm as a fungus bag, putting the cultivation material into the fungus bag, wherein no gap exists between the cultivation material and the wall of the fungus bag, performing high-pressure steam sterilization for 1.5 hours after fastening the mouth of the fungus bag, and naturally cooling to room temperature, taking out of the pot, thus obtaining the ganoderma lucidum bag material;
s3, uniformly mixing 35kg of cottonseed hulls, 14kg of waste coal cinder and 4kg of dry pigeon manure, adding water until the water content is 35 wt%, sending the mixture into a high-pressure steam explosion device, wherein the explosion pressure is 7.6MPa, adding 0.13kg of fermentation inoculum, fermenting until the pH value of the system is 7.1-7.4, adding 50kg of bentonite particles, and uniformly stirring to obtain a nutrient substrate, wherein the concentration of bacillus subtilis in the fermentation inoculum is 1.3 × 109cfu/g, the concentration of the bacillus licheniformis is 1.8 × 109cfu/g；
Controlling the temperature to be 25 ℃ in the first 10-12 days after the ganoderma lucidum bag material is inoculated; then adjusting the temperature to 22 ℃, maintaining the temperature for 22 ℃ to 16-18 days, and then adjusting the temperature to 20.5 ℃;
removing the fungus bag when the hyphae grow to 15cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation mushroom rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 84%, and harvesting after mushroom caps grow until white points disappear.
Ganoderma lucidum was cultivated in the Liuan region of Anhui province by the cultivation method of examples 3-5, and the growth of mycelia was as follows:
| hypha growth rate, mm/d | Growth potential of hypha | Edge uniformity of colony | Roundness of bacterial colony | |
| Example 3 | 13.27 | ++++ | +++ | Round and tidy |
| Example 4 | 12.68 | +++ | ++ | Round and tidy |
| Example 5 | 13.83 | ++++ | +++ | Round and tidy |
Wherein: the hypha growth rate (mm/d) was (average colony diameter-6)/(hypha growth days × 2).
As shown in the above table: by adopting the cultivation method, the growth rate and the growth potential of hyphae are improved, and the edge uniformity and the roundness of bacterial colonies are good.
Extracting ganoderma triterpenes from the ganoderma lucidum obtained from each group, and measuring the content by an HPLC method (after the raw material is crushed, high-concentration ethanol liquid is heated and extracted, after an extracting solution is concentrated, a high performance liquid chromatograph is used for detecting, ganoderma acid is used as a standard substance, and the ganoderma triterpenes content in the raw material is obtained through corresponding absorption peak areas), wherein the following results are obtained:
| average ganoderma triterpene content% | |
| Example 3 | 1.42 |
| Example 4 | 1.55 |
| Example 5 | 1.61 |
As shown in the above table: the ganoderma lucidum obtained by the cultivation method has higher total triterpene content.
In the above experiments, the set-up repetition is used, and each experimental data is the average value of each group repetition.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. A three-dimensional soilless organic cultivation method of lucid ganoderma is characterized by comprising the following steps:
s1, mixing the paper mulberry branches, the sweet potato vines, the rotten wood, the sweet potato powder and the bagasse, crushing, sieving to obtain particles with the particle size of 2-3mm, adding water in the stirring process until the water content is 25-35 wt%, naturally fermenting, adding the tea powder, the gypsum powder, the lime powder, the calcium carbonate, the glucose and the magnesium sulfate, and uniformly mixing to obtain the cultivation material;
s2, filling the cultivation material into fungus bags, wherein no gap exists between the cultivation material and the fungus bags, tightly binding the mouths of the fungus bags, putting the fungus bags into a steamer, sterilizing the fungus bags with high-pressure steam, naturally cooling the fungus bags to room temperature, and taking the fungus bags out of the steamer to obtain ganoderma lucidum bag materials;
s3, after inoculating the lucid ganoderma bag material, controlling the temperature to be 24-26 ℃ in the first 10-12 days; then adjusting the temperature to 21-23 ℃, maintaining the temperature at 21-23 ℃ for 15-20 days, and then adjusting the temperature to 20-21 ℃;
removing the fungus bag when the hypha grows to 14-16cm in the hypha growth period; and (3) after the hyphae are fully distributed, moving the hyphae into a cheese-out shed, placing the cheese-out shed on a three-dimensional cultivation fungus rack, covering each layer with a nutrient medium after arrangement is finished, keeping the humidity in the cheese-out shed at 80-88%, and harvesting after the pileus grows until the white point disappears.
2. The method for three-dimensional soilless organic cultivation of ganoderma lucidum as claimed in claim 1, wherein in S1, the mass ratio of the branch of paper mulberry, the vine of sweet potato, the rotten wood, the powder of sweet potato, the bagasse, the powder of tea, the powder of gypsum, the powder of lime, the calcium carbonate, the glucose and the magnesium sulfate is 100: 10-20: 5-12: 5-10: 1-5: 2-6: 0.5-1.2: 0.5-1.5: 1-2: 1-2: 0.1-0.6.
3. A three-dimensional soilless organic cultivation method for ganoderma lucidum as claimed in claim 1, wherein in S1, the natural fermentation time is 10-16 days, the pile height is 1.5-2m, the pile width is 2.5-3m, and the pile length is 3.2-3.6m in the fermentation process.
4. A three-dimensional soilless organic cultivation method for ganoderma lucidum as claimed in claim 1, wherein in S1, the water content is kept at 25 wt% or more during the fermentation process, and when the temperature is higher than 55 ℃, the stack turning is performed.
5. A three-dimensional soilless organic cultivation method for ganoderma lucidum as claimed in claim 1, wherein in S2, a high pressure polyethylene bag tube with a folded width of 12-14cm, a length of 10-13cm and a thickness of 0.002-0.005cm is used as a fungus bag.
6. The method for the three-dimensional soilless organic cultivation of ganoderma lucidum as claimed in claim 1, wherein in S3, the covering thickness of the nutrient medium is 1.5-2.5 cm.
7. The method for the three-dimensional soilless organic cultivation of the ganoderma lucidum as claimed in claim 1, wherein in the preparation process of the nutrient medium of S3, the cottonseed hulls, the waste coal residues and the dry pigeon droppings are uniformly mixed, water is added until the water content is 30-40 wt%, the mixture is sent into a high-pressure steam explosion device, the explosion pressure is 7.2-8MPa, the fermentation microbial inoculum is added to ferment until the pH value of the system is 7.1-7.4, and the bentonite particles are added to be uniformly stirred to obtain the nutrient medium.
8. The method for the three-dimensional soilless organic cultivation of ganoderma lucidum as claimed in claim 7, wherein the mass ratio of the cotton seed hulls, the waste coal cinder, the dried pigeon manure, the fermentation bacteria agent and the bentonite particles is 30-40: 10-18: 2-6: 0.1-0.15: 30-70.
9. The method for three-dimensional soilless organic cultivation of ganoderma lucidum as claimed in claim 7, wherein the concentration of bacillus subtilis in the fermentation inoculum is 1-1.5 × 109cfu/g, the concentration of the bacillus licheniformis is 1.3-2.2 × 109cfu/g。
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| CN112740865A (en) * | 2020-12-25 | 2021-05-04 | 安徽理工大学 | Method and device for repairing mine post-explosion environment by adopting biological bacteria and moss |
| CN113455285A (en) * | 2021-08-18 | 2021-10-01 | 武夷山元生泰生物科技有限公司 | Tea tree culture medium-based ganoderma lucidum cultivation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112740865A (en) * | 2020-12-25 | 2021-05-04 | 安徽理工大学 | Method and device for repairing mine post-explosion environment by adopting biological bacteria and moss |
| CN113455285A (en) * | 2021-08-18 | 2021-10-01 | 武夷山元生泰生物科技有限公司 | Tea tree culture medium-based ganoderma lucidum cultivation method |
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