CN111700038A - Anti-infection method for predatory mites - Google Patents
Anti-infection method for predatory mites Download PDFInfo
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- CN111700038A CN111700038A CN202010592043.9A CN202010592043A CN111700038A CN 111700038 A CN111700038 A CN 111700038A CN 202010592043 A CN202010592043 A CN 202010592043A CN 111700038 A CN111700038 A CN 111700038A
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 241000238876 Acari Species 0.000 title claims abstract description 25
- 230000002924 anti-infective effect Effects 0.000 title claims abstract description 16
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 claims abstract description 79
- 239000005660 Abamectin Substances 0.000 claims abstract description 79
- 229950008167 abamectin Drugs 0.000 claims abstract description 79
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 68
- 230000001954 sterilising effect Effects 0.000 claims abstract description 60
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 56
- 238000004519 manufacturing process Methods 0.000 claims abstract description 33
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000007789 sealing Methods 0.000 claims abstract description 27
- 239000008367 deionised water Substances 0.000 claims abstract description 16
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 16
- 239000012286 potassium permanganate Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000004887 air purification Methods 0.000 claims abstract description 6
- 238000005507 spraying Methods 0.000 claims abstract description 6
- 238000009423 ventilation Methods 0.000 claims abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 75
- 239000000523 sample Substances 0.000 claims description 40
- 239000005663 Pyridaben Substances 0.000 claims description 35
- DWFZBUWUXWZWKD-UHFFFAOYSA-N pyridaben Chemical compound C1=CC(C(C)(C)C)=CC=C1CSC1=C(Cl)C(=O)N(C(C)(C)C)N=C1 DWFZBUWUXWZWKD-UHFFFAOYSA-N 0.000 claims description 35
- 239000000243 solution Substances 0.000 claims description 28
- 238000007865 diluting Methods 0.000 claims description 15
- 238000002156 mixing Methods 0.000 claims description 15
- 239000012488 sample solution Substances 0.000 claims description 15
- 238000004140 cleaning Methods 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 5
- 229910052751 metal Inorganic materials 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000010025 steaming Methods 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 238000003958 fumigation Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract 1
- 239000007921 spray Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an anti-infection method for predatory mites, which comprises the steps of sealing doors and windows of a predatory mite production workshop, arranging a special air purification and ventilation system, installing an ultraviolet disinfection device, arranging a double-layer sealing door at the door opening to be isolated from the outside, arranging a wind showering room and a dressing room between the two layers of sealing doors, and putting on a disinfected white long jacket and a mask in the dressing room by a worker through the wind showering room before the worker enters the workshop; and (3) sterilizing a production workshop: before the production of the sterilized personnel in the step I, adding deionized water into the sterilized personnel at the dosage of 17.5-22 mL/mu to prepare abamectin diluted solution, and completely preparing the abamectin diluted solution in a production workshopSpraying in azimuth, sealing the workshop for 5-6 days, and spraying the medicine every 10m35mL of formaldehyde and 1g of potassium permanganate are used for fumigation, and the mixture is sealed for 24-28 h. The workshop is fumigated and disinfected, and the wheat bran foundation bed on which the predatory mites survive is subjected to double disinfection, so that the damage of the predatory mites by the avoided foreign matters or other mites is avoided, and the survival rate of the predatory mite cultivation is effectively improved.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to an anti-infection method for predatory mites.
Background
Predatory mites are biological control products for controlling agricultural and forestry pests and pest mites. The use of chemical pesticides in large quantities brings many problems which are difficult to solve to the agriculture and forestry production, such as the continuous enhancement of the drug resistance of pests (mites), the disappearance of natural enemies in fields, pesticide residues in agricultural products, ecological deterioration and the like, and one of the ways for solving the problems is to develop and use biological control products. Compared with other predatory natural enemies, the predatory mites have the obvious advantages of easy artificial mass feeding, higher development speed than that of harmful mites (insects), capability of establishing populations, preference for harmful mites and small insects in a short period, identical habitat with target mites (insects), capability of replacing food in fields and the like.
Disclosure of Invention
The invention aims to provide an anti-infection method for predatory mites, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an anti-infection method for predatory mites comprises the following specific materials: 85-88 parts of deionized water, 1-3 parts of abamectin, 2-3 parts of formaldehyde, 1-2 parts of potassium permanganate, 1-2 parts of pyridaben and 1-2 parts of methanol;
the specific anti-infection steps are as follows:
the method comprises the following steps: sealing doors and windows of a predatory mite production workshop, arranging a special air purification and ventilation system, installing an ultraviolet disinfection device, arranging a double-layer sealing door at a doorway to isolate the door from the outside, arranging a wind showering room and a dressing room between the two layers of sealing doors, and putting on a disinfected white long jacket and a mask in the dressing room before a worker enters the workshop through the wind showering room;
step two: sterilizing a production workshop: before production, the sterilized person in the first step is added with deionized water to prepare abamectin diluted solution in the amount of 17.5-22 mL/mu, and the abamectin diluted solution is sprayed in all directions in a production workshop, and after spraying, the workshop is sealed for 5-6 days, and then every 10m of abamectin diluted solution is sprayed on the workshop3Fumigating 5mL of formaldehyde with 1g of potassium permanganate, and sealing for 24-28 h;
step three: feeding toolAnd (3) disinfection: cleaning all feeding tools, sterilizing, and sealing every 10m in the feeding tool chamber3Fumigating and sterilizing with 5mL of formaldehyde and 1g of potassium permanganate for 12h, and feeding all tools for one batch, and then completely cleaning and sterilizing;
step four: sterilization of culture medium (wheat bran): firstly, packaging wheat bran in large sealed bags by a damp-heat sterilization method, then putting the packaged wheat bran in a steaming cabinet, and sterilizing the wheat bran with steam at 105 ℃ for 45min-1 h;
step five: after the fourth treatment, transferring the sterilized wheat bran into an infrared high-temperature sterilization cabinet by a dry heat sterilization method, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, wherein the thickness of the wheat bran on the conveyor belt is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is used for receiving materials by a large plastic barrel subjected to ultraviolet sterilization;
step six: and (3) detection: detecting before feeding, wherein before wheat bran enters a workshop for feeding, randomly extracting 5 parts of 1g of wheat bran, and inspecting under a viewing mirror;
step seven: during the feeding detection, 5 pots are randomly selected in the south, east and north of a workshop, wheat bran is uniformly mixed in each pot, and 3 parts of wheat bran and 1g of wheat bran are randomly extracted and detected under a viewing mirror.
Further, the air shower room of the first step is arranged in front of the dressing room.
Furthermore, the mite-catching workshop and the feed mite production workshop are required to be separately arranged, the distance between the two workshops is required to be more than 5-7 m, and the fact that the miscellaneous mites enter the production workshop and the predatory mites enter the feed mite workshop is avoided.
Furthermore, after the disinfection operation in the second step is finished, the ultraviolet device is opened for disinfection again one night before production is started.
Further, when the abamectin diluted solution in the second step is prepared, the abamectin and the deionized water are used according to the ratio of 1:1200 according to the workshop area.
Further, the abamectin in the second step is prepared by matching abamectin, methanol and pyridaben, and the preparation steps are as follows: weighing 0.05 part by weight of an abamectin standard sample, placing the abamectin standard sample in a capacity device, dissolving and diluting the abamectin standard sample by using methanol, uniformly mixing to obtain abamectin with the concentration of 1000ppm, then weighing 0.06 part by weight of a pyridaben standard sample, placing the pyridaben standard sample in the capacity device, sucking 4% of abamectin standard sample solution into a pyridaben standard sample bottle, dissolving and diluting the pyridaben standard sample solution to a scale by using methanol, uniformly mixing to obtain abamectin with the concentration of 40ppm, finally taking a sample containing 0.06 part by weight of pyridaben, placing the sample in the capacity device, dissolving and diluting the sample solution to the scale by using methanol, and uniformly mixing to obtain an abamectin solution.
Compared with the prior art, the invention has the beneficial effects that: fully carry out the full aspect disinfection to personnel, workshop and crowd and instrument before getting into the workshop, when keeping driven abamectin mode disinfection, adopt stifling disinfection to the workshop to carry out dual disinfection to the wheat bran foundation bed that the predatory mite survived, make the reproduction of predatory mite cultivate the space stable, high-efficient, the external foreign matter of avoidng or other kinds of mite effectively improve the survival rate that the predatory mite was bred to the infringement of predatory mite.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An anti-infection method for predatory mites comprises the following specific materials: 85 parts of deionized water, 1 part of abamectin, 2 parts of formaldehyde, 1 part of potassium permanganate, 1 part of pyridaben and 1 part of methanol;
the specific anti-infection steps are as follows:
the method comprises the following steps: sealing doors and windows of a predatory mite production workshop, arranging a special air purification and ventilation system, installing an ultraviolet disinfection device, arranging a double-layer sealing door at a doorway to isolate the door from the outside, arranging a wind showering room and a dressing room between the two layers of sealing doors, and putting on a disinfected white long jacket and a mask in the dressing room before a worker enters the workshop through the wind showering room;
step two: sterilizing a production workshop: before production, the disinfected personnel in the step one add deionized water into 17.5 mL/mu of the solution to prepare abamectin diluted solution, spray the abamectin diluted solution in all directions in a production workshop, seal the workshop for 5 days after spraying the abamectin diluted solution, and then spray the abamectin diluted solution to 10m35mL of formaldehyde and 1g of potassium permanganate are used for fumigation, and the mixture is sealed for 24 hours;
step three: sterilizing feeding tools: cleaning all feeding tools, sterilizing, and sealing every 10m in the feeding tool chamber3Fumigating and sterilizing with 5mL of formaldehyde and 1g of potassium permanganate for 12h, and feeding all tools for one batch, and then completely cleaning and sterilizing;
step four: sterilization of culture medium (wheat bran): firstly, packaging wheat bran in large sealed bags by a damp-heat sterilization method, then putting the packaged wheat bran in a steaming cabinet, and sterilizing the wheat bran with steam at 105 ℃ for 45 min;
step five: after the fourth treatment, transferring the sterilized wheat bran into an infrared high-temperature sterilization cabinet by a dry heat sterilization method, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, wherein the thickness of the wheat bran on the conveyor belt is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is used for receiving materials by a large plastic barrel subjected to ultraviolet sterilization;
step six: and (3) detection: detecting before feeding, wherein before wheat bran enters a workshop for feeding, randomly extracting 5 parts of 1g of wheat bran, and inspecting under a viewing mirror;
step seven: during the feeding detection, 5 pots are randomly selected in the south, east and north of a workshop, wheat bran is uniformly mixed in each pot, and 3 parts of wheat bran and 1g of wheat bran are randomly extracted and detected under a viewing mirror.
Wherein, the air shower room of the first step is arranged in front of the dressing room.
Wherein, the workshop of predatory mite needs to be set up separately with the workshop of fodder mite to two workshop distances should reach more than 5 meters, avoid miscellaneous mite to get into the workshop and predatory mite to get into the fodder mite workshop.
And after the disinfection operation in the second step is finished, opening the ultraviolet device for disinfection again one night before production is started.
And B, when preparing the abamectin diluted solution in the step two, using the abamectin and the deionized water according to the ratio of 1:1200 according to the workshop area.
The abamectin in the second step is prepared by matching abamectin, methanol and pyridaben, and the preparation steps are as follows: weighing 0.05 part by weight of an abamectin standard sample, placing the abamectin standard sample in a capacity device, dissolving and diluting the abamectin standard sample by using methanol, uniformly mixing to obtain abamectin with the concentration of 1000ppm, then weighing 0.06 part by weight of a pyridaben standard sample, placing the pyridaben standard sample in the capacity device, sucking 4% of abamectin standard sample solution into a pyridaben standard sample bottle, dissolving and diluting the pyridaben standard sample solution to a scale by using methanol, uniformly mixing to obtain abamectin with the concentration of 40ppm, finally taking a sample containing 0.06 part by weight of pyridaben, placing the sample in the capacity device, dissolving and diluting the sample solution to the scale by using methanol, and uniformly mixing to obtain an abamectin solution.
Example 2
An anti-infection method for predatory mites comprises the following specific materials: 86 parts of deionized water, 1.5 parts of abamectin, 2.5 parts of formaldehyde, 1.5 parts of potassium permanganate, 1.5 parts of pyridaben and 1.5 parts of methanol;
the specific anti-infection steps are as follows:
the method comprises the following steps: sealing doors and windows of a predatory mite production workshop, arranging a special air purification and ventilation system, installing an ultraviolet disinfection device, arranging a double-layer sealing door at a doorway to isolate the door from the outside, arranging a wind showering room and a dressing room between the two layers of sealing doors, and putting on a disinfected white long jacket and a mask in the dressing room before a worker enters the workshop through the wind showering room;
step two: sterilizing a production workshop: before the production of the sterilized personnel in the step I, adding deionized water into the sterilized personnel for preparing abamectin for dilution by using the dosage of 20 mL/muSpraying the solution in workshop, sealing the workshop for 5 days, and spraying the solution every 10m35mL of formaldehyde and 1g of potassium permanganate are used for fumigation, and the mixture is sealed for 27 hours;
step three: sterilizing feeding tools: cleaning all feeding tools, sterilizing, and sealing every 10m in the feeding tool chamber3Fumigating and sterilizing with 5mL of formaldehyde and 1g of potassium permanganate for 12h, and feeding all tools for one batch, and then completely cleaning and sterilizing;
step four: sterilization of culture medium (wheat bran): firstly, packaging wheat bran in large sealed bags by a damp-heat sterilization method, and then putting the packaged wheat bran in a steaming cabinet, and sterilizing the wheat bran with steam at 105 ℃ for 55 min;
step five: after the fourth treatment, transferring the sterilized wheat bran into an infrared high-temperature sterilization cabinet by a dry heat sterilization method, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, wherein the thickness of the wheat bran on the conveyor belt is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is used for receiving materials by a large plastic barrel subjected to ultraviolet sterilization;
step six: and (3) detection: detecting before feeding, wherein before wheat bran enters a workshop for feeding, randomly extracting 5 parts of 1g of wheat bran, and inspecting under a viewing mirror;
step seven: during the feeding detection, 5 pots are randomly selected in the south, east and north of a workshop, wheat bran is uniformly mixed in each pot, and 3 parts of wheat bran and 1g of wheat bran are randomly extracted and detected under a viewing mirror.
Wherein, the air shower room of the first step is arranged in front of the dressing room.
Wherein, the workshop of predatory mite needs to be set up separately with the workshop of fodder mite to two workshop distances should reach more than 6 meters, avoid miscellaneous mite to get into the workshop and predatory mite to get into the fodder mite workshop.
And after the disinfection operation in the second step is finished, opening the ultraviolet device for disinfection again one night before production is started.
And B, when preparing the abamectin diluted solution in the step two, using the abamectin and the deionized water according to the ratio of 1:1200 according to the workshop area.
The abamectin in the second step is prepared by matching abamectin, methanol and pyridaben, and the preparation steps are as follows: weighing 0.05 part by weight of an abamectin standard sample, placing the abamectin standard sample in a capacity device, dissolving and diluting the abamectin standard sample by using methanol, uniformly mixing to obtain abamectin with the concentration of 1000ppm, then weighing 0.06 part by weight of a pyridaben standard sample, placing the pyridaben standard sample in the capacity device, sucking 4% of abamectin standard sample solution into a pyridaben standard sample bottle, dissolving and diluting the pyridaben standard sample solution to a scale by using methanol, uniformly mixing to obtain abamectin with the concentration of 40ppm, finally taking a sample containing 0.06 part by weight of pyridaben, placing the sample in the capacity device, dissolving and diluting the sample solution to the scale by using methanol, and uniformly mixing to obtain an abamectin solution.
Example 3
An anti-infection method for predatory mites comprises the following specific materials: 88 parts of deionized water, 3 parts of abamectin, 3 parts of formaldehyde, 2 parts of potassium permanganate, 2 parts of pyridaben and 2 parts of methanol;
the specific anti-infection steps are as follows:
the method comprises the following steps: sealing doors and windows of a predatory mite production workshop, arranging a special air purification and ventilation system, installing an ultraviolet disinfection device, arranging a double-layer sealing door at a doorway to isolate the door from the outside, arranging a wind showering room and a dressing room between the two layers of sealing doors, and putting on a disinfected white long jacket and a mask in the dressing room before a worker enters the workshop through the wind showering room;
step two: sterilizing a production workshop: before production, the disinfected personnel in the step one add deionized water into the amount of 22 mL/mu to prepare abamectin diluted solution, spray the abamectin diluted solution in all directions in a production workshop, seal the workshop for 6 days after spraying the abamectin diluted solution, and then spray the abamectin diluted solution every 10m35mL of formaldehyde and 1g of potassium permanganate are used for fumigation, and the mixture is sealed for 28 hours;
step three: sterilizing feeding tools: cleaning all feeding tools, sterilizing, and sealing every 10m in the feeding tool chamber3With 5mL of formaldehydeFumigating and sterilizing for 12h by 1g of potassium permanganate, and completely cleaning and sterilizing after feeding all tools for a batch;
step four: sterilization of culture medium (wheat bran): firstly, packaging wheat bran in large sealed bags by a damp-heat sterilization method, then putting the packaged wheat bran in a steaming cabinet, and sterilizing the wheat bran with steam at 105 ℃ for 1 hour;
step five: after the fourth treatment, transferring the sterilized wheat bran into an infrared high-temperature sterilization cabinet by a dry heat sterilization method, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, wherein the thickness of the wheat bran on the conveyor belt is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is used for receiving materials by a large plastic barrel subjected to ultraviolet sterilization;
step six: and (3) detection: detecting before feeding, wherein before wheat bran enters a workshop for feeding, randomly extracting 5 parts of 1g of wheat bran, and inspecting under a viewing mirror;
step seven: during the feeding detection, 5 pots are randomly selected in the south, east and north of a workshop, wheat bran is uniformly mixed in each pot, and 3 parts of wheat bran and 1g of wheat bran are randomly extracted and detected under a viewing mirror.
Wherein, the air shower room of the first step is arranged in front of the dressing room.
Wherein, the workshop of predatory mite needs to be set up separately with the workshop of fodder mite to two workshop distances should reach more than 7 meters, avoid miscellaneous mite to get into the workshop and predatory mite to get into the fodder mite workshop.
And after the disinfection operation in the second step is finished, opening the ultraviolet device for disinfection again one night before production is started.
And B, when preparing the abamectin diluted solution in the step two, using the abamectin and the deionized water according to the ratio of 1:1200 according to the workshop area.
The abamectin in the second step is prepared by matching abamectin, methanol and pyridaben, and the preparation steps are as follows: weighing 0.05 part by weight of an abamectin standard sample, placing the abamectin standard sample in a capacity device, dissolving and diluting the abamectin standard sample by using methanol, uniformly mixing to obtain abamectin with the concentration of 1000ppm, then weighing 0.06 part by weight of a pyridaben standard sample, placing the pyridaben standard sample in the capacity device, sucking 4% of abamectin standard sample solution into a pyridaben standard sample bottle, dissolving and diluting the pyridaben standard sample solution to a scale by using methanol, uniformly mixing to obtain abamectin with the concentration of 40ppm, finally taking a sample containing 0.06 part by weight of pyridaben, placing the sample in the capacity device, dissolving and diluting the sample solution to the scale by using methanol, and uniformly mixing to obtain an abamectin solution.
The working principle of the invention is as follows: fully carry out the full aspect disinfection to personnel, workshop and crowd and instrument before getting into the workshop, when keeping driven abamectin mode disinfection, adopt stifling disinfection to the workshop to carry out dual disinfection to the wheat bran foundation bed that the predatory mite survived, make the reproduction of predatory mite cultivate the space stable, high-efficient, the external foreign matter of avoidng or other kinds of mite effectively improve the survival rate that the predatory mite was bred to the infringement of predatory mite.
The survival comparison table of predatory mites cultured by the anti-infection method and traditionally cultured is as follows:
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. An anti-infection method for predatory mites is characterized by comprising the following specific materials: 85-88 parts of deionized water, 1-3 parts of abamectin, 2-3 parts of formaldehyde, 1-2 parts of potassium permanganate, 1-2 parts of pyridaben and 1-2 parts of methanol;
the specific anti-infection steps are as follows:
the method comprises the following steps: sealing doors and windows of a predatory mite production workshop, arranging a special air purification and ventilation system, installing an ultraviolet disinfection device, arranging a double-layer sealing door at a doorway to isolate the door from the outside, arranging a wind showering room and a dressing room between the two layers of sealing doors, and putting on a disinfected white long jacket and a mask in the dressing room before a worker enters the workshop through the wind showering room;
step two: sterilizing a production workshop: before production, the sterilized person in the first step is added with deionized water to prepare abamectin diluted solution in the amount of 17.5-22 mL/mu, and the abamectin diluted solution is sprayed in all directions in a production workshop, and after spraying, the workshop is sealed for 5-6 days, and then every 10m of abamectin diluted solution is sprayed on the workshop3Fumigating 5mL of formaldehyde with 1g of potassium permanganate, and sealing for 24-28 h;
step three: sterilizing feeding tools: cleaning all feeding tools, sterilizing, and sealing every 10m in the feeding tool chamber3Fumigating and sterilizing with 5mL of formaldehyde and 1g of potassium permanganate for 12h, and feeding all tools for one batch, and then completely cleaning and sterilizing;
step four: sterilization of culture medium (wheat bran): firstly, packaging wheat bran in large sealed bags by a damp-heat sterilization method, then putting the packaged wheat bran in a steaming cabinet, and sterilizing the wheat bran with steam at 105 ℃ for 45min-1 h;
step five: after the fourth treatment, transferring the sterilized wheat bran into an infrared high-temperature sterilization cabinet by a dry heat sterilization method, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, wherein the thickness of the wheat bran on the conveyor belt is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is used for receiving materials by a large plastic barrel subjected to ultraviolet sterilization;
step six: and (3) detection: detecting before feeding, wherein before wheat bran enters a workshop for feeding, randomly extracting 5 parts of 1g of wheat bran, and inspecting under a viewing mirror;
step seven: during the feeding detection, 5 pots are randomly selected in the south, east and north of a workshop, wheat bran is uniformly mixed in each pot, and 3 parts of wheat bran and 1g of wheat bran are randomly extracted and detected under a viewing mirror.
2. The method of claim 1, wherein the method further comprises the step of: the air shower room in the first step is arranged in front of the dressing room.
3. The method of claim 1, wherein the method further comprises the step of: the mite-catching workshop and the feed mite production workshop are separately arranged, the distance between the two workshops reaches more than 5-7 m, and the fact that the mites enter the production workshops and the predatory mites enter the feed mite workshops is avoided.
4. The method of claim 1, wherein the method further comprises the step of: and after the disinfection operation in the second step is finished, opening the ultraviolet device for disinfection again one night before production is started.
5. The method of claim 1, wherein the method further comprises the step of: when the abamectin dilution solution in the second step is prepared, the abamectin and the deionized water are used according to the ratio of 1:1200 according to the area of a workshop.
6. The method of claim 1, wherein the method further comprises the step of: the abamectin in the second step is prepared by matching abamectin, methanol and pyridaben, and the preparation steps are as follows: weighing 0.05 part by weight of an abamectin standard sample, placing the abamectin standard sample in a capacity device, dissolving and diluting the abamectin standard sample by using methanol, uniformly mixing to obtain abamectin with the concentration of 1000ppm, then weighing 0.06 part by weight of a pyridaben standard sample, placing the pyridaben standard sample in the capacity device, sucking 4% of abamectin standard sample solution into a pyridaben standard sample bottle, dissolving and diluting the pyridaben standard sample solution to a scale by using methanol, uniformly mixing to obtain abamectin with the concentration of 40ppm, finally taking a sample containing 0.06 part by weight of pyridaben, placing the sample in the capacity device, dissolving and diluting the sample solution to the scale by using methanol, and uniformly mixing to obtain an abamectin solution.
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| CN112841091A (en) * | 2021-01-05 | 2021-05-28 | 赣南师范大学 | Method for researching induction of congenital heart disease of animal by pyridaben and application of pyridaben |
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