CN106386827A - Plant disease bactericide high in safety and efficiency, and preparation method thereof - Google Patents
Plant disease bactericide high in safety and efficiency, and preparation method thereof Download PDFInfo
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- CN106386827A CN106386827A CN201610793524.XA CN201610793524A CN106386827A CN 106386827 A CN106386827 A CN 106386827A CN 201610793524 A CN201610793524 A CN 201610793524A CN 106386827 A CN106386827 A CN 106386827A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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Abstract
The invention belongs to the field of agriculture, and especially relates to a plant disease bactericide high in safety and efficiency, and a preparation method thereof. The plant disease bactericide high in safety and efficiency is prepared from, by weight, 3 to 15% of a polyamino oligosaccharide, 0.3 to 1% of glycine betaine, 0.1 to 1% of compound sodium nitrophenolate, 0.4 to 1.5% of sodium salicylate, 5 to 12% of matrine, and the balance pure water. The ratio of the plant disease bactericide is reasonable; mutual complementation of the ingredients is achieved; synergistic effect is achieved and is obvious; plant diseases can be prevented effectively; when the plant disease bactericide is applied on crops, active factors capable of resisting plant diseases are generated under induction of crops, so that plant diseases can be prevented and controlled effectively; dosage is low; no harm effect on people and livestock is caused; no environment pollution is caused; and crop specific disease resistance is not caused via long term or a plurality times of induction.
Description
Technical field
The invention belongs to agriculture field, particularly to one kind safely and efficiently plant disease antibacterial and preparation method thereof.
Background technology
Plant disease is a big limiting factor of higher yield of crops, stable yields, high-quality.In order to prevent and treat various plant diseases, agriculture
The people have in a large number using chemical bactericide.Although chemical bactericide using effectively preventing disease, also give agricultural product
Hidden danger has been buried in safety and ecological environment, such as the enhancing of plant disease Drug resistance, drug residue, soil and water resources pollution aggravation etc..With
The continuous improvement of people's living standard, people are also increasingly serious to the worry of chemistry security and worry, and exploitation is efficient, low
Poison, low-residual, environment amenable pesticide new product substitute chemical pesticide has become the target of various countries pesticide worker pursuit.?
It has been found that the use of biological pesticide can solve above potential hazard in chemical pesticide succedaneum development process, biological
Pesticide refers to the class harmful organisms such as insect, pathogenic bacteria, weeds, nematicide prevented and treated using biological living or its metabolite
Pesticidal preparations.Compare traditional chemical bactericide, biological preparation has the characteristics that safety, noresidue.However, it is existing on the market at present
Some biological pesticide bactericidal effects do not meet the production needs of people, also do not reach the requirement replacing traditional chemical antibacterial.
Content of the invention
The present invention is in order to make up the deficiencies in the prior art, there is provided one kind prepare simply, reasonable recipe, effect substantially, peace
Entirely effectively, noresidue and eco-friendly safely and efficiently plant disease antibacterial and preparation method thereof.
The present invention is achieved through the following technical solutions：
One kind safely and efficiently plant disease antibacterial, is made up of following components in percentage by weight：Poly- amino oligosaccharide 3
～15%, glycine betaine 0.3～1%, compound sodium nitrophenolate 0.1～1%, sodium salicylate 0.4～1.5%, matrine 5～12%, purification
Water complements to 100%.
The preparation method of this safely and efficiently plant disease antibacterial, using following steps：
(1) prepare poly- amino oligosaccharide：
(1-1) purified water of 88～92 mass parts and the low-molecular weight oligo sugar of 8-12 weight portion, this purification of labelling are weighed
Water is purified water A, and purified water A and low-molecular weight oligo sugar are added in reactor A, stirs 20-30 minute, makes quality dense
Spend the oligomerization sugar juice for 8%-12%；
(1-2) sodium hydroxide and the 2-5 mass parts of 3-7 mass parts are added in the prepared oligomerization sugar juice of step (1-1)
Isopropanol, be heated to 70-80 DEG C, add the ammonium chloride of 10-15 mass parts, after keeping this temperature 4-6 hour, temperature is dropped
To room temperature, it is slowly added to acetic acid until solution ph is 7 and produces flocculent deposit；Washed with ethanol or water.
(1-3) precipitate prepared for step (1-2) is dried to constant weight, obtain final product poly- amino oligosaccharide；
(2) prepare this safely and efficiently plant disease antibacterial：
(2-1) weigh the prepared poly- amino oligosaccharide of the step (1-3) that mass fraction is 3～15%, and described quality
The glycine betaine 0.3～1% of fraction, compound sodium nitrophenolate 0.1～1%, sodium salicylate 0.4～1.5%, matrine 5～12%, surplus is used
Purified water polishing, and this purified water is labeled as purified water B；
(2-2) under room temperature, the 70% of purified water B is added in reactor B, then successively step (2-1) is weighed
The poly- amino oligosaccharide of described mass fraction, matrine, glycine betaine, compound sodium nitrophenolate, sodium salicylate add in reactor B, and
All stir 30 minutes after every kind of component adds reactor B, after making the solution mix homogeneously in reactor, add lower a kind of group
Point, finally remaining purified water B is added in reactor B, polishing to 100%, obtain final product described in safely and efficiently plant disease
Antibacterial.
Usage and dosage：Calculated with every mu of consumption of plant disease antibacterial finished product 50g/ of the present invention, be watered 700-900 times
Afterwards, in crops their early stage foliage-spray.
The performance of each component of the present invention is as follows：
Poly- amino oligosaccharide：Oligosaccharide refers to the compound being polymerized containing 2-10 glycosidic bond, and oligosaccharide can be made
For plant immune system activator, the fast reaction of coordinate plant growth growth and cell surface.This product is using low point
The oligosaccharide of son amount, under the conditions of specific reason, change, by ammonium ion chemical modification so as to each again on C2 position and C6 position gather
, there are three amino so on each sugared ring, become poly- amino oligosaccharide in unification amino.Because being polymerized more ammonia
Base, makes that the activity of oligosaccharide is higher, and effect is more preferable.
Glycine betaine：Glycine betaine is a kind of alkaloid, has strong moisture pick-up propertiess, biodegradation low to skin irritation
Property is good, has excellent stain removal bactericidal function.
Compound sodium nitrophenolate：Compound sodium nitrophenolate is to penetrate into rapidly plant after a kind of strength cell vitalizing agent, with plant contact
Interior, promote the protoplasm flowing of cell, improve cell viability, improve disease-resistant, the pest-resistant, drought resisting of crop, waterlogging-resistant, cold-resistant, salt resistance
Alkali, the anti-adversity ability such as resistant to lodging, have efficiently, low toxicity, noresidue, applied widely, have no side effect, concentration wide ranges etc.
Advantage.
Sodium salicylate：Sodium salicylate is white flakes or powder, is mainly used in analgesic and medicine for rheumatism, also serves as organic conjunction
Become the reagent of free acid in raw material, preservative, survey gastric juice, obtained with crystallizing by salicylic acid alkali.Its preparation method：By bigcatkin willow
Acid and sodium bicarbonate intersect in addition distilled water, and temperature keeps 60 DEG C, and keeps in acid state, is simultaneously introduced appropriate ethylenediamine
Tetraacethyl (EDTA) and sodium hydrosulfite.It is warming up to 85 DEG C, react half an hour.Qualified reactant liquor is sent into ebullated bed through filtering, in
85 DEG C of dry sodium salicylate.
Matrine：Matrine is through organic solvent extraction systems such as ethanol by the dry root of leguminous plant Radix Sophorae Flavescentiss, plant, fruit
Become, be alkaloid, be a kind of low toxicity, low-residual, environmental protection type agricultural chemical, there is insecticidal activity, bactericidal activity, coordinate plant growth
The several functions such as function, have good prevention effect.Matrine, as being a kind of botanical pesticide, can divide rapidly in the Nature
Solution, final product is carbon dioxide and water.And, matrine is not because multiple chemical substance collective effects are so as to be easily caused
Evil thing develops immunity to drugs, energy life-time service, corresponding insect directly will not be killed completely, but control insect biotic population number
Amount will not badly influence production and the procreation of this plant population.
The beneficial effect of the present invention safely and efficiently plant disease antibacterial：
1st, this safely and efficiently plant disease antibacterial reasonable mixture ratio, by being complementary to one another between each composition, combines association
Same onset, potentiation is notable, effectively the downy mildew of preventing and treating Fructus Cucumidis sativi, Fructus Lycopersici esculenti, Fructus Capsici, Fructus Solani melongenae, Citrullus vulgariss, Fructus Melo etc., powdery mildew,
The harm to crops for the mycosises such as gray mold anthrax.Act on and can directly kill pathogenic bacteria during crops and induce crops certainly
Body produces the activity factor resisting plant disease, thus effectively killing and controlling plant disease.Field test shows, the present invention pacifies
, as a kind of biological preparation, consumption is low for efficient plant disease antibacterial entirely, and prevention effect is better than traditional chemical bactericide, right
Person poultry harmless, free from environmental pollution, long-term or multiple induction will not make crops produce specificity disease resistance.
2nd, poly- amino oligosaccharide used in the present invention be using low-molecular-weight oligosaccharide under specific physico chemical factor, lead to
Cross chemical modification so as on C2 position and C6 position each one amino of repolymerization so that on each sugared ring exist three amino,
More amino can greatly improve the activity of oligosaccharide, and can be bonded the molecule of more materials, controls its rate of release, increasing
Plus its safety and lasting effect.Meanwhile, poly- amino oligosaccharide used in the present invention and matrine, glycine betaine, compound sodium nitrophenolate, water
Poplar acid sodium is all neutral or alkalescence material, and between each group is divided, the compatibility, good stability effect duration are long.Matrine, glycine betaine, multiple
Nitre phenol sodium, sodium salicylate all have sterilization disease-resistant effect, can improve the anti-adversity ability of crop, play benefit to poly- amino oligosaccharide
Fill, potentiation.And, glycine betaine is good wetting agent, and compound sodium nitrophenolate can increase the permeability of medicinal liquid, can be greatly improved and kill
The preventive effect of microbial inoculum.
Specific embodiment
With reference to embodiment, the invention will be further described, but the invention is not limited in this, the system in embodiment
Preparation Method is customary preparation methods, no longer describes in detail.
Embodiment 1：
This safely and efficiently plant disease antibacterial, is made up of following components in percentage by weight：Poly- amino oligosaccharide 3,000
Gram, 1 kilogram of glycine betaine, 0.1 kilogram of compound sodium nitrophenolate, 1.5 kilograms of sodium salicylate, 12 kilograms of matrine and 82.4 kilograms of purified water.
The preparation method of this safely and efficiently plant disease antibacterial, step is as follows：
(1) prepare poly- amino oligosaccharide：
(1-1) 90 kilograms of purified water and 10 kilograms of low-molecular-weight are weighed, this purified water of labelling is purified water A, will be pure
Change water A and low-molecular weight oligo sugar is added in reactor A, stir 25 minutes, make the oligosaccharide that mass concentration is 10% molten
Liquid；
(1-2) sodium hydroxide of 5 mass parts and the different of 4 mass parts are added in the prepared oligomerization sugar juice of step (1-1)
Propanol, is heated to 75 DEG C, adds the ammonium chloride of 13 mass parts, keeps this temperature after 5 hours, cools the temperature to room temperature, slowly
Add acetic acid until solution ph is 7 and produces flocculent deposit；Washed with ethanol or water；
(1-3) precipitate prepared for step (1-2) is dried to constant weight, obtain final product poly- amino oligosaccharide；
(2) prepare this safely and efficiently plant disease antibacterial：
(2-1) weigh 3 kilograms prepared of poly- amino oligosaccharide of step (1-3), and 1 kilogram of glycine betaine, compound sodium nitrophenolate 0.1
Kilogram, 1.5 kilograms of sodium salicylate, 12 kilograms of matrine, 82.4 kilograms of purified water, and this purified water is labeled as purified water B；
(2-2) under room temperature, the 70% of purified water B is added in reactor B, then successively step (2-1) is weighed
The poly- amino oligosaccharide of described mass fraction, matrine, glycine betaine, compound sodium nitrophenolate, sodium salicylate add in reactor B, and
All stir 30 minutes after every kind of component adds reactor B, after making the solution mix homogeneously in reactor, add lower a kind of group
Point, finally remaining purified water B is added in reactor B, polishing to 100%, obtain final product described in safely and efficiently plant disease
Antibacterial.
Usage and dosage：Calculated with every mu of consumption of plant disease antibacterial finished product 50g/ of the present invention, be watered 700-900 times
Afterwards, in crops their early stage foliage-spray.
Embodiment 2：
It is made up of following components in percentage by weight：9 kilograms of poly- amino oligosaccharide, 0.3 kilogram of glycine betaine, compound sodium nitrophenolate
0.77 kilogram, 0.95 kilogram of sodium salicylate, 8.5 kilograms of matrine, 80.48 kilograms of purified water.
Preparation method and usage and dosage are with embodiment 1.
Embodiment 3：
It is made up of following components in percentage by weight：15 kilograms of poly- amino oligosaccharide, 0.82 kilogram of glycine betaine, compound sodium nitrophenolate
0.32 kilogram, 0.67 kilogram of sodium salicylate, 5 kilograms of matrine, 78.19 kilograms of purified water.
Preparation method and usage and dosage are with embodiment 1.
Embodiment 4：
It is made up of following components in percentage by weight：12 kilograms of poly- amino oligosaccharide, 0.47 kilogram of glycine betaine, compound sodium nitrophenolate
0.55 kilogram, 0.4 kilogram of sodium salicylate, 6.7 kilograms of matrine, 79.88 kilograms of purified water.
Preparation method and usage and dosage are with embodiment 1.
Embodiment 5：
It is made up of following components in percentage by weight：6 kilograms of poly- amino oligosaccharide, 0.65 kilogram of glycine betaine, compound sodium nitrophenolate 1
Kilogram, 1.22 kilograms of sodium salicylate, 10.2 kilograms of matrine, 80.93 kilograms of purified water.
Preparation method and usage and dosage are with embodiment 1.
Test example：
In order to verify the effect of the present invention safely and efficiently plant disease antibacterial, present inventor has performed fungistatic effect is real
Test and field test.
1st, fungistatic effect test
Take gained of the present invention safely and efficiently plant disease antibacterial (embodiment 1-5), and have studied it to multiple common
Plant disease pathogen bacteriostasis.
1.1 strain material
Used by test, pathogen is respectively Fulvia fulva, is isolatable from leaf muld of tomato scab；Melon monofilament shell powdery mildew, separates
From powdery mildew of cucumber scab；Botrytis cinerea, is isolatable from Botrytis cinerea scab；Eggplant brown line Phomopsis, are isolatable from the brown stricture of vagina of Fructus Solani melongenae
Disease；Fungi Imperfecti melon thorn disk spore, is isolatable from Fructus Melo anthrax scab；Citrullus vulgariss phyllosticta, is isolatable from Citrullus vulgariss leaf spot scab；Herba Spinaciae frost
Mould pathogenic bacteria, is isolatable from downy mildew of spinach scab；F.graminearum schw, is isolatable from wheat scab.(by Agriculture in Shandong Province microorganism weight
Point laboratory separates and preserves).
1.2 experimental technique
With Fulvia fulva, melon monofilament shell powdery mildew, Botrytis cinerea, eggplant brown line Phomopsis, Fungi Imperfecti melon thorn disk spore, Citrullus vulgariss
Phyllosticta, downy mildew of spinach bacterium, F.graminearum schw are experimental strain, carry out activation culture respectively, after growing single bacterium colony, respectively with connecing
Plant a little thalline of ring picking in the test tube filling physiological saline solution, shaken well, it is prepared into suspension.In aseptic condition
Under, add appropriate culture medium in the culture dish of 90mm, after solidification, add 100 microlitres of bacterium solution, coating uniformly, stands 20-30 minute.
By gained of the present invention safely and efficiently plant disease antibacterial and other plant disease antibacterial (disease of such as going out prestige suspending agent, is purchased from
Shi Qi insecticide factory of Zhongshan city) make the circle of a diameter of 0.4cm respectively, be placed on solid medium, on same culture dish according to
Secondary placement other plant disease antibacterial and gained of the present invention safely and efficiently plant disease antibacterial, are repeated 10 times, antibacterial exists
24h is cultivated in 37 DEG C of constant incubator.Observe each sample and have or not bacterial growth with culture medium contact surface, and it is straight to measure inhibition zone
Footpath size.Using SPSS v11.5 software data processing, data represents, concrete condition is shown in Table 1 with mean ± standard deviation (x ± S)：
Table 1：The contact antibacterial antibacterial circle diameter (unit to strain for each sample：cm；Sample diameter 0.4cm)
As can be seen from Table 1, melon monofilament shell powdery mildew, Botrytis cinerea, Fungi Imperfecti melon thorn disk spore, watermelon leaf point are being suppressed
Mould this kind of pathogen aspect, the present invention safely and efficiently plant disease antibacterial and other plant disease antibacterial (disease of such as going out
Prestige suspending agent) it is respectively provided with preferable contact bacteriostasis, the contact surface of sample and culture medium all no bacterial growths, but institute of the present invention
The fungistatic effect of the safely and efficiently plant disease antibacterial obtaining is higher, such as this product safely and efficiently plant disease antibacterial
Melon monofilament shell powdery mildew is contacted with antibacterial antibacterial circle diameter is 0.749 ± 0.0098cm, and other plant disease antibacterial pair
It is 0.674 ± 0.0124m that melon monofilament shell powdery mildew contacts antibacterial antibacterial circle diameter.Importantly, brown to Fulvia fulva, eggplant
The bactericidal effect aspect of the pathogen such as stricture of vagina Phomopsis, downy mildew of spinach bacterium, F.graminearum schw, the present invention safely and efficiently phytopathy
The fungistatic effect of evil antibacterial is significantly larger than other plant disease antibacterial, such as this product safely and efficiently plant disease sterilization
It is 0.768 ± 0.0137cm that agent contacts antibacterial antibacterial circle diameter to F.graminearum schw, and other plant disease antibacterial is to cereal sickle
It is 0.379 ± 0.0135cm that spore contacts antibacterial antibacterial circle diameter.
To sum up, gained of the present invention safely and efficiently plant disease antibacterial and existing other plant disease antibacterial phase
Its fungistatic effect is more significantly, and all has significant fungistatic effect to various pathogen, has broad-spectrum, is worth for ratio
Use is widely popularized on market.
2nd, field test
Experimental field be arranged on Taian Taishan Academy of Forestry Sciences proving ground, respectively plantation Fructus Melo, Fructus Lycopersici esculenti, Fructus Cucumidis sativi,
Fructus Capsici, Fructus Solani melongenae, all plant in booth, and in mid-May, 2010 sowing, 10 the middle ten days in 2010 are colonized, and the area of trial zone is
50 square meters, are equally divided into 5 cells, respectively Muskmelon Planting cell, tomato planting cell, green cucumber cell, pepper planting
Cell and Fructus Solani melongenae plantation cell, the area of each plantation cell is 10 square meters, is respectively intended to plant Fructus Melo, Fructus Lycopersici esculenti, Fructus Cucumidis sativi, peppery
Using plastic sheeting isolation between green pepper, Fructus Solani melongenae, and each plantation cell.
Respectively with Fungi Imperfecti melon thorn disk spore, Fulvia fulva, melon monofilament shell powdery mildew, Botrytis cinerea, eggplant brown line Phomopsis
For experimental strain, carry out activation culture respectively, after growing single bacterium colony, respectively with a little thalline of inoculating loop picking in filling aseptic life
In the test tube of reason saline, shaken well, it is prepared into Fungi Imperfecti melon thorn disk spore suspension, Fulvia fulva suspension, melon monofilament shell white
Powder bacterium suspension, Botrytis cinerea suspension, eggplant brown line Phomopsis suspension.
Mid-May is sowed, and waters, and treats that seedling grows after 25 days, little in Muskmelon Planting cell, tomato planting respectively
Area, green cucumber cell, pepper planting cell and Fructus Solani melongenae are planted cell and are sprayed the Fungi Imperfecti melon thorn disk spore suspension having prepared
Liquid, Fulvia fulva suspension, melon monofilament shell white lead bacterium suspension, Botrytis cinerea suspension and eggplant brown line Phomopsis suspension,
Simultaneously by each plantation little be distinguished into two parts (respectively matched group and experimental group), isolated using plastic sheeting, often
The matched group part of individual cell is equally divided into 5 zonules again.Carry out first time dispenser (note during disease symptom to appear：Dispenser
Time might not be unanimously), the experimental group of each plantation cell uses gained plant disease antibacterial finished product 0.8g of the present invention, converts
After 700-900 times of water, spray in corps leaf surface；The quality such as is sprayed respectively in 5 zonules of each plantation cell matched group
75% Bravo wettable powder, polyoxin, 70% thiophanate-methyl wettable powder add 60% azoles ether Carbatene moisture
Shot agent, captan, virus must gram, after being equally watered 700-900 times, spray on crop plant.Each the first in the group time
10 days after dispenser, then carry out second dispenser (note：Spraying time is also not necessarily consistent), the experimental group of each plantation cell uses
Gained plant disease antibacterial finished product 1.2g of the present invention, after being watered 700-900 times, sprays on crop plant；Each plantation
75% Bravo wettable powder of quality, polyoxin, the 70% methyl sulfur such as are sprayed respectively in 5 zonules of cell matched group
Bacterium spirit wettable powder add 60% azoles ether Carbatene water dispersible granules, captan, virus must gram.
Disease mensure is carried out using five point sampling, selectes 5 plants at random in the experimental group of each cell and matched group respectively
Plant, investigates every plant of whole blades respectively.Fructus Melo anthrax, leaf muld of tomato, powdery mildew of cucumber, peppery is investigated respectively before dispenser
The morbidity radix of green pepper gray mold and Fructus Solani melongenae Phomopisis vexans, investigation statisticses prevention effect when 10 days after second dispenser, calculate suppression ratio,
Concrete condition such as table 2 below.
The field control effect of table 2 present invention safely and efficiently plant disease antibacterial and other antibacterial
As can be known from Table 2, on the one hand, safely and efficiently plant disease antibacterial all has relatively the present invention to plurality of plant diseases
Good fungistatic effect, has broad-spectrum, and mostly other traditional antibacterial are for certain plant disease, to other plant
Disease is almost without fungistatic effect.On the other hand, the fungistatic effect of the present invention safely and efficiently plant disease antibacterial is than its homicide
The fungistatic effect of microbial inoculum is more significantly, such as the bacteriostasis to Fructus Melo anthrax：Plant disease using gained of the present invention is killed
The bacteriostasis rate of microbial inoculum all reaches more than 75%, and also only reaches 66.9% using the bacteriostasis rate of 75% Bravo wettable powder,
And other antibacterial to the plant disease of Fructus Melo anthrax almost without fungistatic effect.
To sum up, safely and efficiently plant disease antibacterial is better than passing the present invention to the antibacterial prevention effect of plurality of plant diseases
The antibacterial of system, worth marketing uses.
Finally it should be noted that embodiment is the optimum specific embodiment of the present invention, it is not limited to this
Invention, although being described in detail to the present invention with reference to the foregoing embodiments, for a person skilled in the art, its according to
So the technical scheme described in foregoing embodiments can be modified, or wherein some technical characteristics are carried out with equivalent replacing
Change.All any modification, equivalent substitution and improvement within the spirit and principles in the present invention, made etc., should be included in the present invention
Protection domain within.
Claims (2)
1. one kind safely and efficiently plant disease antibacterial, is characterized in that：It is made up of following components in percentage by weight：Poly- amino
Oligosaccharide 3～15%, glycine betaine 0.3～1%, compound sodium nitrophenolate 0.1～1%, sodium salicylate 0.4～1.5%, matrine 5～
12%, purified water complements to 100%.
2. a kind of preparation method of safely and efficiently plant disease antibacterial as claimed in claim 1, is characterized in that：Including such as
Lower step：
(1) prepare poly- amino oligosaccharide：
(1-1) purified water of 88～92 mass parts and the low-molecular weight oligo sugar of 8-12 weight portion are weighed, this purified water of labelling is
Purified water A, purified water A and low-molecular weight oligo sugar are added in reactor A, stir 20-30 minute, making mass concentration is
The oligomerization sugar juice of 8%-12%；
(1-2) sodium hydroxide of 3-7 mass parts and the different of 2-5 mass parts are added in the prepared oligomerization sugar juice of step (1-1)
Propanol, is heated to 70-80 DEG C, adds the ammonium chloride of 10-15 mass parts, after keeping this temperature 4-6 hour, cools the temperature to room
Temperature, is slowly added to acetic acid until solution ph is 7 and produces flocculent deposit；Washed with ethanol or water；
(1-3) precipitate prepared for step (1-2) is dried to constant weight, obtain final product poly- amino oligosaccharide；
(2) prepare this safely and efficiently plant disease antibacterial：
(2-1) weigh the prepared poly- amino oligosaccharide of the step (1-3) that mass fraction is 3～15%, and described mass fraction
Glycine betaine 0.3～1%, compound sodium nitrophenolate 0.1～1%, sodium salicylate 0.4～1.5%, matrine 5～12%, surplus purification
Water polishing, and this purified water is labeled as purified water B；
(2-2), under room temperature, the 70% of purified water B is added in reactor B, then successively by step (2-1) weigh described
The poly- amino oligosaccharide of mass fraction, matrine, glycine betaine, compound sodium nitrophenolate, sodium salicylate add in reactor B, and every
Plant after component adds reactor B and all stir 30 minutes, add a kind of lower component after making the solution mix homogeneously in reactor,
Afterwards remaining purified water B is added in reactor B, polishing to 100%, obtain final product described in safely and efficiently plant disease sterilization
Agent.
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| TWI700034B (en) * | 2017-12-24 | 2020-08-01 | 日商日本歐愛特農業科技股份有限公司 | Synergistic bactericide composition containing nitrophenol compound and bactericidal active compound |
| CN112384068A (en) * | 2018-05-15 | 2021-02-19 | 丹斯塔发酵股份公司 | Use of a combination of a fungicide and glycine betaine for controlling fungal plant pathogens |
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| TWI700034B (en) * | 2017-12-24 | 2020-08-01 | 日商日本歐愛特農業科技股份有限公司 | Synergistic bactericide composition containing nitrophenol compound and bactericidal active compound |
| CN112384068A (en) * | 2018-05-15 | 2021-02-19 | 丹斯塔发酵股份公司 | Use of a combination of a fungicide and glycine betaine for controlling fungal plant pathogens |
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