CN111699240A - 用于细胞培养缩放的系统和方法 - Google Patents

用于细胞培养缩放的系统和方法 Download PDF

Info

Publication number
CN111699240A
CN111699240A CN201980009086.5A CN201980009086A CN111699240A CN 111699240 A CN111699240 A CN 111699240A CN 201980009086 A CN201980009086 A CN 201980009086A CN 111699240 A CN111699240 A CN 111699240A
Authority
CN
China
Prior art keywords
diameter
impeller
bioprocessing
container
bioreactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980009086.5A
Other languages
English (en)
Chinese (zh)
Inventor
C·布劳
N·琼斯
M·史密斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of CN111699240A publication Critical patent/CN111699240A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/113—Propeller-shaped stirrers for producing an axial flow, e.g. shaped like a ship or aircraft propeller
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/051—Stirrers characterised by their elements, materials or mechanical properties
    • B01F27/054—Deformable stirrers, e.g. deformed by a centrifugal force applied during operation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/07—Stirrers characterised by their mounting on the shaft
    • B01F27/071—Fixing of the stirrer to the shaft
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/114—Helically shaped stirrers, i.e. stirrers comprising a helically shaped band or helically shaped band sections
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/21—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by their rotating shafts
    • B01F27/211—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by their rotating shafts characterised by the material of the shaft
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/21—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by their rotating shafts
    • B01F27/212—Construction of the shaft
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/21—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by their rotating shafts
    • B01F27/213—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by their rotating shafts characterised by the connection with the drive
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/23—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by the orientation or disposition of the rotor axis
    • B01F27/232—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by the orientation or disposition of the rotor axis with two or more rotation axes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/91—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with propellers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/92—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with helices or screws
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
    • B01F35/32—Driving arrangements
    • B01F35/32005—Type of drive
    • B01F35/3204—Motor driven, i.e. by means of an electric or IC motor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/40—Mounting or supporting mixing devices or receptacles; Clamping or holding arrangements therefor
    • B01F35/41—Mounting or supporting stirrer shafts or stirrer units on receptacles
    • B01F35/412—Mounting or supporting stirrer shafts or stirrer units on receptacles by supporting both extremities of the shaft
    • B01F35/4121—Mounting or supporting stirrer shafts or stirrer units on receptacles by supporting both extremities of the shaft at the top and at the bottom of the receptacle, e.g. for performing a conical orbital movement about a vertical axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/40—Mounting or supporting mixing devices or receptacles; Clamping or holding arrangements therefor
    • B01F35/43—Supporting receptacles on frames or stands
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/513—Flexible receptacles, e.g. bags supported by rigid containers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
    • B01F2035/35—Use of other general mechanical engineering elements in mixing devices
    • B01F2035/352—Bearings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B01F23/2336—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the location of the place of introduction of the gas relative to the stirrer
    • B01F23/23362—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the location of the place of introduction of the gas relative to the stirrer the gas being introduced under the stirrer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms 
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
    • B01F35/32—Driving arrangements
    • B01F35/321—Disposition of the drive
    • B01F35/3214—Disposition of the drive at the upper side of the axis, e.g. driving the stirrer from the top of a receptacle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Mechanical Engineering (AREA)
  • Aviation & Aerospace Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)
  • Accessories For Mixers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980009086.5A 2018-01-17 2019-01-03 用于细胞培养缩放的系统和方法 Pending CN111699240A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201862618215P 2018-01-17 2018-01-17
US62/618,215 2018-01-17
US201862670934P 2018-05-14 2018-05-14
US62/670,934 2018-05-14
US201862712343P 2018-07-31 2018-07-31
US62/712,343 2018-07-31
PCT/US2019/012120 WO2019143476A1 (en) 2018-01-17 2019-01-03 System and method for cell culture scaling

Publications (1)

Publication Number Publication Date
CN111699240A true CN111699240A (zh) 2020-09-22

Family

ID=65199609

Family Applications (4)

Application Number Title Priority Date Filing Date
CN201980009126.6A Active CN111615554B (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009084.6A Pending CN111615553A (zh) 2018-01-17 2019-01-03 可配置流体混合系统壳体和支撑硬件
CN202411081171.1A Pending CN118767728A (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009086.5A Pending CN111699240A (zh) 2018-01-17 2019-01-03 用于细胞培养缩放的系统和方法

Family Applications Before (3)

Application Number Title Priority Date Filing Date
CN201980009126.6A Active CN111615554B (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009084.6A Pending CN111615553A (zh) 2018-01-17 2019-01-03 可配置流体混合系统壳体和支撑硬件
CN202411081171.1A Pending CN118767728A (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法

Country Status (5)

Country Link
US (7) US11293003B2 (enExample)
EP (3) EP3740560A1 (enExample)
JP (7) JP7241761B2 (enExample)
CN (4) CN111615554B (enExample)
WO (3) WO2019143476A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112246173A (zh) * 2020-09-29 2021-01-22 广州市爱百伊生物技术有限公司 一种配料罐及生产线
TWI822173B (zh) * 2021-08-17 2023-11-11 日商日揮股份有限公司 運算裝置、控制裝置、培養系統以及培養系統的設計方法

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7241761B2 (ja) * 2018-01-17 2023-03-17 ライフ テクノロジーズ コーポレーション 構成可能な流体混合システムのハウジングおよび支援ハードウェア
USD894377S1 (en) * 2019-01-03 2020-08-25 Life Technologies Corporation Tube management assembly
US11535421B2 (en) * 2019-12-30 2022-12-27 Global Life Sciences Solutions Usa Llc System and method for packaging a bioprocessing bag and associated components, and packaging for a bioprocessing bag
BR112022013998A2 (pt) 2020-02-03 2022-10-11 Life Technologies Corp Sistemas de mistura de fluidos com rotores modulares e métodos relacionados
US12005403B2 (en) * 2020-08-06 2024-06-11 Signature Science, Llc Mixing chamber apparatus for high-volume sampling
CN116348584A (zh) * 2020-10-08 2023-06-27 环球生命科技咨询美国有限责任公司 生物处理系统和用于减小生物处理系统中细胞切变的设备
US11649654B2 (en) * 2020-10-12 2023-05-16 Cytiva Us Llc Tank with door locking mechanism
CN117241876A (zh) * 2021-04-16 2023-12-15 Emd密理博公司 一次性柔性喷射器
WO2023278137A1 (en) * 2021-06-30 2023-01-05 Corning Incorporated Macro-sparger for benchtop bioreactors
CN114210520B (zh) * 2021-12-08 2023-03-14 佛山市亨力豪机械有限公司 一种淋胶机
CN114917784A (zh) * 2022-04-15 2022-08-19 哈尔滨工业大学 一种用于熔融石英半球谐振子的抗腐蚀搅拌装置
CN116983871B (zh) * 2023-09-20 2023-12-01 常州登达适医疗科技有限公司 一种气液混合搅拌装置及其搅拌方法
CN117298909A (zh) * 2023-10-30 2023-12-29 吉安市中香天然植物有限公司 一种负载植物精油的水凝胶的制备方法
CN119457018B (zh) * 2025-01-14 2025-04-29 贵州大学 一种用于建筑爬架链条的金属棒铸造装置

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102112595A (zh) * 2008-08-06 2011-06-29 普莱克斯技术有限公司 用于控制哺乳动物细胞培养过程的系统和方法
CN102307984A (zh) * 2009-02-09 2012-01-04 英国龙沙生物医药股份有限公司 用于培养哺乳动物细胞的生物反应器
US20130288346A1 (en) * 2011-01-11 2013-10-31 Xcellerex Inc. Linearly scalable single use bioreactor system
US20150118753A1 (en) * 2012-04-18 2015-04-30 Life Technologies Corporation Methods and apparatus for gas stream mass transfer with a liquid
US20170183617A1 (en) * 2015-12-29 2017-06-29 Life Technologies Corporation Fluid mixing system with laterally displaced flexible drive lines and methods of use
US20170349874A1 (en) * 2016-06-03 2017-12-07 Lonza Ltd Single Use Bioreactor
US20180010082A1 (en) * 2016-06-03 2018-01-11 Lonza Ltd Bioreactor With Higher Agitation Rates

Family Cites Families (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US94614A (en) 1869-09-07 Improvement in turbine water-wheels
US1752833A (en) 1930-04-01 Thexla u
US659345A (en) 1900-04-21 1900-10-09 Clifford E Ivins Beater.
US1227671A (en) 1915-12-21 1917-05-29 Borden S Condensed Milk Company Food and beverage mixer.
US1436172A (en) 1922-04-27 1922-11-21 Holmgren Gottfrid Mixing device
US1778188A (en) 1927-07-15 1930-10-14 Guy Walter De Motte Ice-cream attachment for mechanical refrigerators
US1711114A (en) 1928-04-21 1929-04-30 Joseph W Hunt Agitator
US1954093A (en) 1931-09-11 1934-04-10 Mark W Nelson Flexible shaft paint mixing apparatus or device
US1898724A (en) 1931-09-23 1933-02-21 Albert R Gifford Means for making ice cream in an electric refrigerator
FR782935A (fr) 1934-09-29 1935-07-05 Pompe, compresseur ou machines similaires centrifuges à pales orientables
US2295098A (en) * 1939-11-02 1942-09-08 Jr Fritz G Cornell Seal for liquid treating apparatus
US2552057A (en) 1948-12-11 1951-05-08 Abbott Lab Mixing apparatus
US2896926A (en) 1956-01-10 1959-07-28 Chapman Harold Eric Device for mixing or treating fluids or free-flowing powders
NL272530A (enExample) * 1960-12-15
LU39997A1 (enExample) 1961-04-10
NL300358A (enExample) 1963-07-02
GB1096701A (en) * 1963-11-28 1967-12-29 Cerebos Foods Ltd Improvements in apparatus for whipping and foaming materials
US3455540A (en) 1968-01-04 1969-07-15 Emil G Marcmann Stirring device
US3559962A (en) 1968-06-10 1971-02-02 Binks Res & Dev Stirring device
US3692427A (en) 1970-07-13 1972-09-19 Colortex Sa High speed mixing impeller
US4083653A (en) 1975-11-07 1978-04-11 Stiffler Hugh A Stirring device
US4355906A (en) 1981-04-03 1982-10-26 Bellco Glass Inc. Stirring apparatus for cell culture
USD273709S (en) 1981-06-15 1984-05-01 Hollister Incorporated Urinary drainage bag
US4722608A (en) 1985-07-30 1988-02-02 General Signal Corp. Mixing apparatus
US4712922A (en) * 1986-01-03 1987-12-15 Core Industries Inc. Material mixing apparatus
USD336034S (en) 1990-11-05 1993-06-01 Rebilas Richard R Condiment bottle refilling container
JP3177551B2 (ja) 1993-04-06 2001-06-18 カワセインダストリィー株式会社 混合機及びその攪拌装置
US5411331A (en) 1993-06-01 1995-05-02 Westinghouse Electric Corporation Device for promoting gravity flow of non-free-flowing solids
US5411311A (en) 1994-07-05 1995-05-02 Ford Motor Company Attachment apparatus for automotive structure brace
JP3584344B2 (ja) 1994-07-26 2004-11-04 信越化学工業株式会社 多糖類の製造装置および製造方法
EP0698662B1 (en) 1994-07-26 2001-06-06 Shin-Etsu Chemical Co., Ltd. Apparatus and method for the production of xanthan gum
US5454797A (en) 1994-08-12 1995-10-03 Haswell; John N. Combined pelvic tray, workstation and fluid collection device
USD373709S (en) 1995-08-11 1996-09-17 Shyh-Shyang Leu Saucer
EP1716885A3 (en) 1997-05-09 2006-11-15 Pall Corporation Connector assemblies, fluid systems, and methods for making a connection
US5885001A (en) 1997-06-13 1999-03-23 Cadence Technologies, Inc. Agitator assembly with a retractable blade assembly
FR2766091A1 (fr) 1997-07-18 1999-01-22 Transgene Sa Composition antitumorale a base de polypeptide immunogene de localisation cellulaire modifiee
US6083587A (en) 1997-09-22 2000-07-04 Baxter International Inc. Multilayered polymer structure for medical products
US5896989A (en) 1998-02-20 1999-04-27 Bracco Research Usa Flexible medical container packaging
US5941636A (en) 1998-03-19 1999-08-24 Lu; Chen-Yi Mixer having mixing blades capable of expanding automatically
USD439328S1 (en) 1999-03-03 2001-03-20 Plm Ab Container for infusion and other liquids
US20030053838A1 (en) 1999-03-22 2003-03-20 Mike Whitney Geometric and perforated paint mixer and paint roller cleaner
JP4369121B2 (ja) 2001-02-06 2009-11-18 リーブテック，インコーポレイテッド 容器内に密封された材料を滅菌状態下で混合するための装置及び方法
JP2002234023A (ja) 2001-02-08 2002-08-20 Iwamoto Hiroyoshi ミキサ
US7025318B2 (en) 2001-03-19 2006-04-11 Baxter International Inc. Container support
US6659132B2 (en) 2001-03-19 2003-12-09 Baxter International Inc. Gas permeable sterile closure
US20020131654A1 (en) 2001-03-19 2002-09-19 Smith Sidney T. Large volume flexible container
US6670171B2 (en) 2001-07-09 2003-12-30 Wheaton Usa, Inc. Disposable vessel
US20030077466A1 (en) 2001-10-19 2003-04-24 Smith Sidney T. Multilayered polymer structure
US6884186B2 (en) 2002-10-25 2005-04-26 Playhard Sports, Llc Game projectile dispensing device
WO2004087320A2 (en) 2003-03-28 2004-10-14 Hyclone Laboratories, Inc. Fluid dispensing bins and related methods
US20040245124A1 (en) * 2003-06-03 2004-12-09 Hurst William E. Preformed mixing bag for dry powder, apparatus, method and system for using same
US20040245144A1 (en) * 2003-06-03 2004-12-09 Hurst William E. Preformed mixing bag for dry powder, apparatus, method and system for using same
US7441940B2 (en) 2003-10-23 2008-10-28 Sport Usa, Llc Collapsible mixing wand
KR20070015178A (ko) 2004-04-27 2007-02-01 백스터 인터내셔널 인코포레이티드 교반-탱크 반응기 시스템
USD679023S1 (en) 2004-07-19 2013-03-26 Thermotek, Inc. Foot wrap
EP1656866A1 (fr) * 2004-11-12 2006-05-17 Nestec S.A. Appareil et méthode pour la préparation de mousse à partir d'un liquide alimentaire à base de lait
CN2764472Y (zh) * 2005-01-19 2006-03-15 南京德朔实业有限公司 搅拌头
US20060196501A1 (en) 2005-03-02 2006-09-07 Hynetics Llc Systems and methods for mixing and sparging solutions and/or suspensions
US8603805B2 (en) 2005-04-22 2013-12-10 Hyclone Laboratories, Inc. Gas spargers and related container systems
US7879599B2 (en) 2005-04-22 2011-02-01 Hyclone Laboratories, Inc. Tube ports and related container systems
US7682067B2 (en) 2005-04-22 2010-03-23 Hyclone Laboratories, Inc. Mixing systems and related mixers
WO2007011745A2 (en) 2005-07-14 2007-01-25 Randy Kaas Whisk for attachment to electric mixer
WO2008040567A1 (en) 2006-10-03 2008-04-10 Artelis Flexible mixing bag, mixing device and mixing system
ATE401951T1 (de) 2005-10-21 2008-08-15 Abb Research Ltd Mischer
KR20080074896A (ko) 2005-10-26 2008-08-13 레브테크 인크. 혼합기와 분사기를 구비한 생물 반응기
US7487688B2 (en) 2006-03-20 2009-02-10 Hyclone Laboratories, Inc. Sampling ports and related container systems
CN100496687C (zh) 2006-04-19 2009-06-10 京东方科技集团股份有限公司 颜料料浆的搅拌设备
EP2010310A4 (en) * 2006-04-21 2010-03-31 Advanced Tech Materials SUBSTANCE MIXING SYSTEMS AND DEVICES AND METHODS FOR THEIR MANUFACTURE
DE102006022306B4 (de) 2006-05-11 2009-06-25 Sartorius Stedim Biotech Gmbh Vibrationsmischer
DE202006009493U1 (de) 2006-06-14 2007-07-26 Monolith GmbH Bürosysteme Rollenschneidgerät zum Beschneiden von Blattgut
US8439554B2 (en) 2006-07-13 2013-05-14 Randy Kaas Whisk attachment
US8008065B2 (en) 2006-08-02 2011-08-30 Finesse Solutions, Llc. Disposable bioreactor vessel port
US20080116012A1 (en) * 2006-11-09 2008-05-22 Ferguson Brock E Platform Lift for a Vehicle
US20080151686A1 (en) 2006-11-14 2008-06-26 Charles Meadows Mixing bag for use with nonabrasive stir bar
USD662212S1 (en) 2007-04-10 2012-06-19 Thermotek, Inc. Butterfly wrap
CN101715363B (zh) 2007-06-04 2014-07-09 通用电气医疗集团生物科学生物方法公司 用于混合容器内盛物的装置
DE102007061664B4 (de) 2007-12-18 2009-11-19 Sartorius Stedim Biotech Gmbh Behälter mit flexibler Wandung
WO2009115926A2 (en) * 2008-03-19 2009-09-24 Sartorius Stedim Biotech Gmbh Method of mixing
WO2009143925A2 (de) 2008-04-17 2009-12-03 Sartorius Stedim Biotech Gmbh Flexibler beutel mit einer mischvorrichtung
US8381780B2 (en) * 2008-05-22 2013-02-26 Xcellerex, Inc. Lift and support assemblies and methods for collapsible bag containers of vessels and bioreactors
DE102008025507A1 (de) 2008-05-28 2009-12-03 Sartorius Stedim Biotech Gmbh Mischsystem
DE102008025508A1 (de) 2008-05-28 2009-12-03 Sartorius Stedim Biotech Gmbh Mischsystem
US8192071B2 (en) 2008-07-16 2012-06-05 Sartorius Stedim Biotech Gmbh Agitator apparatus with collapsible impeller
US20100105125A1 (en) 2008-10-24 2010-04-29 Bioprocessh20 Llc Systems, apparatuses and methods for cultivating microorganisms and mitigation of gases
DE102008058338B4 (de) 2008-11-20 2010-11-11 Sartorius Stedim Biotech Gmbh Rührer für Bioreaktor
EP2229853A1 (en) * 2009-03-19 2010-09-22 Green Lane Products Ltd. Milk frothing device
US8348737B2 (en) 2009-05-21 2013-01-08 Everett Page Method for conducting an online contest
JP2011023084A (ja) 2009-07-17 2011-02-03 Toshiba Corp 半導体記憶装置
TW201611639A (zh) 2009-10-01 2016-03-16 內數位專利控股公司 功綠控制方法及裝置
US8506198B2 (en) 2010-02-01 2013-08-13 Hyclone Laboratories, Inc. Self aligning coupling for mixing system
US8641314B2 (en) 2010-02-01 2014-02-04 Hyclone Laboratories, Inc. Quick coupling for drive shaft
US8455242B2 (en) 2010-02-22 2013-06-04 Hyclone Laboratories, Inc. Mixing system with condenser
SE534819C2 (sv) 2010-05-06 2012-01-10 Itt Mfg Enterprises Inc Omröraraggregat för rötkammare
US8960486B2 (en) 2010-06-16 2015-02-24 Life Technologies Corporation Fluid mixing system with hangers
JP5758009B2 (ja) 2010-11-30 2015-08-05 ミテコ アクチエンゲゼルシャフトＭｉｔｅｃｏ ＡＧ 攪拌設備及び攪拌方法
JP5686345B2 (ja) 2011-03-29 2015-03-18 日本電産シンポ株式会社 土練装置
CA2848652C (en) 2011-09-16 2020-04-14 Thomas Erdenberger Single-use mixing and bioreactor systems
CN106635740B (zh) 2011-09-30 2019-03-15 生命科技股份有限公司 具有膜喷洒器的容器
WO2013063129A1 (en) 2011-10-25 2013-05-02 Hyclone Laboratories, Inc. Fluid mixing systems with adjustable mixing element
US20140370588A1 (en) 2011-12-09 2014-12-18 Atmi Packaging, Inc. Filtration apparatus for continuous perfusion
JP6094478B2 (ja) 2012-02-29 2017-03-15 東レ株式会社 色調に優れたポリアミド樹脂組成物
US9463133B2 (en) 2012-04-02 2016-10-11 Maverick Sports Medicine, Inc. Deep tissue massage roller
EP2836295B1 (en) 2012-04-06 2019-08-28 Life Technologies Corporation Fluid mixing system with flexible drive line
CN202606066U (zh) 2012-04-25 2012-12-19 林美复合材料(苏州)有限公司 一种搅拌机
KR102103338B1 (ko) 2013-04-19 2020-05-29 이엠디 밀리포어 코포레이션 일회용 바이오리액터 내의 신축성 필름 배플
SG10201910334WA (en) 2013-09-16 2020-01-30 Genentech Inc Bioreactors with multiple or adjustable -position agitator designs
CN106999796B (zh) * 2014-10-24 2020-09-25 生命科技股份有限公司 以声学方式沉淀的液-液样品纯化系统
JP2018508234A (ja) 2015-02-17 2018-03-29 ジェネア アイピー ホールディングス ピーティーワイ リミテッド 生物学的試料を動的に培養するための方法および装置
US9714405B2 (en) * 2015-04-14 2017-07-25 Pall Corporation Bioprocessing container tube system and method of use
CN107438478B (zh) * 2015-04-16 2021-11-16 环球生命科技咨询美国有限责任公司 生物处理混合器
KR102485147B1 (ko) 2015-07-06 2023-01-06 엘지전자 주식회사 변형 가능한 디스플레이 장치 및 그의 동작 방법
US9683208B2 (en) 2015-07-31 2017-06-20 Ernest Louis Stadler Horizontal single use pressurizable modular multi-agitator microbial fermentator
US10836989B2 (en) 2015-10-16 2020-11-17 Global Life Sciences Solutions Usa Llc Disposable container, mixing system and packaging
USD830544S1 (en) 2016-05-17 2018-10-09 Detectachem Llc Receptacle
USD857188S1 (en) 2016-09-29 2019-08-20 Mine Survival, Inc. Rebreather device
USD824042S1 (en) 2016-11-10 2018-07-24 Viacyte, Inc. Perforated cell encapsulation device
USD828653S1 (en) 2016-12-14 2018-09-11 Brandon Penland Treatment applicator
US11788048B2 (en) * 2017-09-19 2023-10-17 Life Technologies Corporation Systems and methods for a collapsible chamber with foldable mixing element
USD870315S1 (en) 2017-11-09 2019-12-17 Hemocue Ab Cuvette
JP7241761B2 (ja) * 2018-01-17 2023-03-17 ライフ テクノロジーズ コーポレーション 構成可能な流体混合システムのハウジングおよび支援ハードウェア
US20230150756A1 (en) * 2021-11-17 2023-05-18 Coldsnap, Corp. Rapidly Cooling Food and Drinks

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102112595A (zh) * 2008-08-06 2011-06-29 普莱克斯技术有限公司 用于控制哺乳动物细胞培养过程的系统和方法
CN102307984A (zh) * 2009-02-09 2012-01-04 英国龙沙生物医药股份有限公司 用于培养哺乳动物细胞的生物反应器
US20130288346A1 (en) * 2011-01-11 2013-10-31 Xcellerex Inc. Linearly scalable single use bioreactor system
US20150118753A1 (en) * 2012-04-18 2015-04-30 Life Technologies Corporation Methods and apparatus for gas stream mass transfer with a liquid
US20170183617A1 (en) * 2015-12-29 2017-06-29 Life Technologies Corporation Fluid mixing system with laterally displaced flexible drive lines and methods of use
US20170349874A1 (en) * 2016-06-03 2017-12-07 Lonza Ltd Single Use Bioreactor
US20180010082A1 (en) * 2016-06-03 2018-01-11 Lonza Ltd Bioreactor With Higher Agitation Rates

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112246173A (zh) * 2020-09-29 2021-01-22 广州市爱百伊生物技术有限公司 一种配料罐及生产线
TWI822173B (zh) * 2021-08-17 2023-11-11 日商日揮股份有限公司 運算裝置、控制裝置、培養系統以及培養系統的設計方法

Also Published As

Publication number Publication date
CN118767728A (zh) 2024-10-15
CN111615554A (zh) 2020-09-01
US11584910B2 (en) 2023-02-21
JP7241761B2 (ja) 2023-03-17
US11293003B2 (en) 2022-04-05
JP7465331B2 (ja) 2024-04-10
EP3740561A1 (en) 2020-11-25
JP2023107983A (ja) 2023-08-03
US12227732B2 (en) 2025-02-18
JP7566955B2 (ja) 2024-10-15
EP3740559A1 (en) 2020-11-25
JP2021510524A (ja) 2021-04-30
CN111615554B (zh) 2024-08-16
EP3740560A1 (en) 2020-11-25
US20220243165A1 (en) 2022-08-04
JP2024071715A (ja) 2024-05-24
JP2021511036A (ja) 2021-05-06
WO2019143477A1 (en) 2019-07-25
US20190218496A1 (en) 2019-07-18
US20230091993A1 (en) 2023-03-23
US20190218494A1 (en) 2019-07-18
WO2019143478A1 (en) 2019-07-25
US20220145231A1 (en) 2022-05-12
US12467030B2 (en) 2025-11-11
US20250154445A1 (en) 2025-05-15
WO2019143476A1 (en) 2019-07-25
US11352598B2 (en) 2022-06-07
JP2023026438A (ja) 2023-02-24
CN111615553A (zh) 2020-09-01
JP7223012B2 (ja) 2023-02-15
US20190217261A1 (en) 2019-07-18
US12460171B2 (en) 2025-11-04
JP2023067984A (ja) 2023-05-16
JP2021511033A (ja) 2021-05-06

Similar Documents

Publication Publication Date Title
CN111699240A (zh) 用于细胞培养缩放的系统和方法
US12331273B2 (en) Fluid mixing systems with adjustable mixing element
US11162062B2 (en) Methods and apparatus for gas stream mass transfer with a liquid
US8980623B2 (en) Cell culture and mixing vessel
EP3062919B1 (en) Modular aeration device and method
US9499290B2 (en) Stationary bubble reactors
EP1687394B1 (en) Cell culture system
JP2015515270A (ja) 加圧された単回使用バイオリアクタを旋回させること
CN107438478A (zh) 生物处理混合器
US20120295248A1 (en) Systems and methods for dynamic gas control in a disposable vessel
JP2022513217A (ja) バイオプロセスシステムのための羽根車およびスパージャ組立体
US20240093137A1 (en) Sparger assemblies for a bioprocessing system
CN113727775A (zh) 用于生物处理系统的叶轮组件
JP2024523555A (ja) ベンチトップ型バイオリアクター用マクロスパージャー

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination