CN111699240A - 用于细胞培养缩放的系统和方法 - Google Patents

用于细胞培养缩放的系统和方法 Download PDF

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Publication number
CN111699240A
CN111699240A CN201980009086.5A CN201980009086A CN111699240A CN 111699240 A CN111699240 A CN 111699240A CN 201980009086 A CN201980009086 A CN 201980009086A CN 111699240 A CN111699240 A CN 111699240A
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China
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diameter
impeller
bioprocessing
container
bioreactor
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CN201980009086.5A
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English (en)
Chinese (zh)
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C·布劳
N·琼斯
M·史密斯
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Life Technologies Corp
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Life Technologies Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Mechanical Engineering (AREA)
  • Aviation & Aerospace Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)
  • Accessories For Mixers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980009086.5A 2018-01-17 2019-01-03 用于细胞培养缩放的系统和方法 Pending CN111699240A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201862618215P 2018-01-17 2018-01-17
US62/618,215 2018-01-17
US201862670934P 2018-05-14 2018-05-14
US62/670,934 2018-05-14
US201862712343P 2018-07-31 2018-07-31
US62/712,343 2018-07-31
PCT/US2019/012120 WO2019143476A1 (en) 2018-01-17 2019-01-03 System and method for cell culture scaling

Publications (1)

Publication Number Publication Date
CN111699240A true CN111699240A (zh) 2020-09-22

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Family Applications (4)

Application Number Title Priority Date Filing Date
CN202411081171.1A Pending CN118767728A (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009126.6A Active CN111615554B (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009084.6A Pending CN111615553A (zh) 2018-01-17 2019-01-03 可配置流体混合系统壳体和支撑硬件
CN201980009086.5A Pending CN111699240A (zh) 2018-01-17 2019-01-03 用于细胞培养缩放的系统和方法

Family Applications Before (3)

Application Number Title Priority Date Filing Date
CN202411081171.1A Pending CN118767728A (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009126.6A Active CN111615554B (zh) 2018-01-17 2019-01-03 包括具有叶轮附件的螺旋混合组件的流体混合系统和使用方法
CN201980009084.6A Pending CN111615553A (zh) 2018-01-17 2019-01-03 可配置流体混合系统壳体和支撑硬件

Country Status (5)

Country Link
US (7) US11293003B2 (enExample)
EP (3) EP3740559A1 (enExample)
JP (7) JP7223012B2 (enExample)
CN (4) CN118767728A (enExample)
WO (3) WO2019143478A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
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CN112246173A (zh) * 2020-09-29 2021-01-22 广州市爱百伊生物技术有限公司 一种配料罐及生产线
TWI822173B (zh) * 2021-08-17 2023-11-11 日商日揮股份有限公司 運算裝置、控制裝置、培養系統以及培養系統的設計方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3740559A1 (en) 2018-01-17 2020-11-25 Life Technologies Corporation System and method for cell culture scaling
USD894377S1 (en) * 2019-01-03 2020-08-25 Life Technologies Corporation Tube management assembly
US11535421B2 (en) * 2019-12-30 2022-12-27 Global Life Sciences Solutions Usa Llc System and method for packaging a bioprocessing bag and associated components, and packaging for a bioprocessing bag
BR112022013998A2 (pt) * 2020-02-03 2022-10-11 Life Technologies Corp Sistemas de mistura de fluidos com rotores modulares e métodos relacionados
US12005403B2 (en) * 2020-08-06 2024-06-11 Signature Science, Llc Mixing chamber apparatus for high-volume sampling
US20230295554A1 (en) * 2020-10-08 2023-09-21 Global Life Sciences Solutions Usa Llc Bioprocessing system and apparatus for reducing cell shear in a bioprocessing system
US11649654B2 (en) * 2020-10-12 2023-05-16 Cytiva Us Llc Tank with door locking mechanism
CA3216823A1 (en) * 2021-04-16 2022-10-20 Emd Millipore Corporation Single use flexible sparger
JP2024523555A (ja) * 2021-06-30 2024-06-28 コーニング インコーポレイテッド ベンチトップ型バイオリアクター用マクロスパージャー
CN114210520B (zh) * 2021-12-08 2023-03-14 佛山市亨力豪机械有限公司 一种淋胶机
CN114917784A (zh) * 2022-04-15 2022-08-19 哈尔滨工业大学 一种用于熔融石英半球谐振子的抗腐蚀搅拌装置
CN116983871B (zh) * 2023-09-20 2023-12-01 常州登达适医疗科技有限公司 一种气液混合搅拌装置及其搅拌方法
CN117298909A (zh) * 2023-10-30 2023-12-29 吉安市中香天然植物有限公司 一种负载植物精油的水凝胶的制备方法
CN119457018B (zh) * 2025-01-14 2025-04-29 贵州大学 一种用于建筑爬架链条的金属棒铸造装置

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102112595A (zh) * 2008-08-06 2011-06-29 普莱克斯技术有限公司 用于控制哺乳动物细胞培养过程的系统和方法
CN102307984A (zh) * 2009-02-09 2012-01-04 英国龙沙生物医药股份有限公司 用于培养哺乳动物细胞的生物反应器
US20130288346A1 (en) * 2011-01-11 2013-10-31 Xcellerex Inc. Linearly scalable single use bioreactor system
US20150118753A1 (en) * 2012-04-18 2015-04-30 Life Technologies Corporation Methods and apparatus for gas stream mass transfer with a liquid
US20170183617A1 (en) * 2015-12-29 2017-06-29 Life Technologies Corporation Fluid mixing system with laterally displaced flexible drive lines and methods of use
US20170349874A1 (en) * 2016-06-03 2017-12-07 Lonza Ltd Single Use Bioreactor
US20180010082A1 (en) * 2016-06-03 2018-01-11 Lonza Ltd Bioreactor With Higher Agitation Rates

Family Cites Families (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1752833A (en) 1930-04-01 Thexla u
US94614A (en) 1869-09-07 Improvement in turbine water-wheels
US659345A (en) 1900-04-21 1900-10-09 Clifford E Ivins Beater.
US1227671A (en) 1915-12-21 1917-05-29 Borden S Condensed Milk Company Food and beverage mixer.
US1436172A (en) 1922-04-27 1922-11-21 Holmgren Gottfrid Mixing device
US1778188A (en) 1927-07-15 1930-10-14 Guy Walter De Motte Ice-cream attachment for mechanical refrigerators
US1711114A (en) 1928-04-21 1929-04-30 Joseph W Hunt Agitator
US1954093A (en) 1931-09-11 1934-04-10 Mark W Nelson Flexible shaft paint mixing apparatus or device
US1898724A (en) 1931-09-23 1933-02-21 Albert R Gifford Means for making ice cream in an electric refrigerator
FR782935A (fr) 1934-09-29 1935-07-05 Pompe, compresseur ou machines similaires centrifuges à pales orientables
US2295098A (en) * 1939-11-02 1942-09-08 Jr Fritz G Cornell Seal for liquid treating apparatus
US2552057A (en) 1948-12-11 1951-05-08 Abbott Lab Mixing apparatus
US2896926A (en) 1956-01-10 1959-07-28 Chapman Harold Eric Device for mixing or treating fluids or free-flowing powders
NL272530A (enExample) 1960-12-15
DE1287562B (enExample) 1961-04-10 1969-01-23
NL300358A (enExample) 1963-07-02
GB1096701A (en) * 1963-11-28 1967-12-29 Cerebos Foods Ltd Improvements in apparatus for whipping and foaming materials
US3455540A (en) 1968-01-04 1969-07-15 Emil G Marcmann Stirring device
US3559962A (en) 1968-06-10 1971-02-02 Binks Res & Dev Stirring device
US3692427A (en) 1970-07-13 1972-09-19 Colortex Sa High speed mixing impeller
US4083653A (en) 1975-11-07 1978-04-11 Stiffler Hugh A Stirring device
US4355906A (en) 1981-04-03 1982-10-26 Bellco Glass Inc. Stirring apparatus for cell culture
USD273709S (en) 1981-06-15 1984-05-01 Hollister Incorporated Urinary drainage bag
US4722608A (en) 1985-07-30 1988-02-02 General Signal Corp. Mixing apparatus
US4712922A (en) * 1986-01-03 1987-12-15 Core Industries Inc. Material mixing apparatus
USD336034S (en) 1990-11-05 1993-06-01 Rebilas Richard R Condiment bottle refilling container
JP3177551B2 (ja) 1993-04-06 2001-06-18 カワセインダストリィー株式会社 混合機及びその攪拌装置
US5411331A (en) 1993-06-01 1995-05-02 Westinghouse Electric Corporation Device for promoting gravity flow of non-free-flowing solids
US5411311A (en) 1994-07-05 1995-05-02 Ford Motor Company Attachment apparatus for automotive structure brace
DE69521175T2 (de) 1994-07-26 2001-11-08 Shin-Etsu Bio Inc., San Diego Verfahren zur Herstellung von Xanthangummi
JP3584344B2 (ja) 1994-07-26 2004-11-04 信越化学工業株式会社 多糖類の製造装置および製造方法
US5454797A (en) 1994-08-12 1995-10-03 Haswell; John N. Combined pelvic tray, workstation and fluid collection device
USD373709S (en) 1995-08-11 1996-09-17 Shyh-Shyang Leu Saucer
EP1716885A3 (en) 1997-05-09 2006-11-15 Pall Corporation Connector assemblies, fluid systems, and methods for making a connection
US5885001A (en) 1997-06-13 1999-03-23 Cadence Technologies, Inc. Agitator assembly with a retractable blade assembly
FR2766091A1 (fr) 1997-07-18 1999-01-22 Transgene Sa Composition antitumorale a base de polypeptide immunogene de localisation cellulaire modifiee
US6083587A (en) 1997-09-22 2000-07-04 Baxter International Inc. Multilayered polymer structure for medical products
US5896989A (en) 1998-02-20 1999-04-27 Bracco Research Usa Flexible medical container packaging
US5941636A (en) 1998-03-19 1999-08-24 Lu; Chen-Yi Mixer having mixing blades capable of expanding automatically
USD439328S1 (en) 1999-03-03 2001-03-20 Plm Ab Container for infusion and other liquids
US20030053838A1 (en) 1999-03-22 2003-03-20 Mike Whitney Geometric and perforated paint mixer and paint roller cleaner
WO2002062458A1 (en) 2001-02-06 2002-08-15 Levtech, Inc. Apparatus and method for mixing materials sealed in a container under sterile conditions
JP2002234023A (ja) 2001-02-08 2002-08-20 Iwamoto Hiroyoshi ミキサ
US6659132B2 (en) 2001-03-19 2003-12-09 Baxter International Inc. Gas permeable sterile closure
US7025318B2 (en) * 2001-03-19 2006-04-11 Baxter International Inc. Container support
US20020131654A1 (en) 2001-03-19 2002-09-19 Smith Sidney T. Large volume flexible container
US6670171B2 (en) 2001-07-09 2003-12-30 Wheaton Usa, Inc. Disposable vessel
US20030077466A1 (en) 2001-10-19 2003-04-24 Smith Sidney T. Multilayered polymer structure
US6884186B2 (en) 2002-10-25 2005-04-26 Playhard Sports, Llc Game projectile dispensing device
WO2004087320A2 (en) 2003-03-28 2004-10-14 Hyclone Laboratories, Inc. Fluid dispensing bins and related methods
US20040245124A1 (en) * 2003-06-03 2004-12-09 Hurst William E. Preformed mixing bag for dry powder, apparatus, method and system for using same
US20040245144A1 (en) * 2003-06-03 2004-12-09 Hurst William E. Preformed mixing bag for dry powder, apparatus, method and system for using same
US7441940B2 (en) 2003-10-23 2008-10-28 Sport Usa, Llc Collapsible mixing wand
KR20070015178A (ko) 2004-04-27 2007-02-01 백스터 인터내셔널 인코포레이티드 교반-탱크 반응기 시스템
USD679023S1 (en) 2004-07-19 2013-03-26 Thermotek, Inc. Foot wrap
EP1656866A1 (fr) * 2004-11-12 2006-05-17 Nestec S.A. Appareil et méthode pour la préparation de mousse à partir d'un liquide alimentaire à base de lait
CN2764472Y (zh) * 2005-01-19 2006-03-15 南京德朔实业有限公司 搅拌头
US20060196501A1 (en) 2005-03-02 2006-09-07 Hynetics Llc Systems and methods for mixing and sparging solutions and/or suspensions
US7879599B2 (en) 2005-04-22 2011-02-01 Hyclone Laboratories, Inc. Tube ports and related container systems
US7682067B2 (en) 2005-04-22 2010-03-23 Hyclone Laboratories, Inc. Mixing systems and related mixers
US8603805B2 (en) 2005-04-22 2013-12-10 Hyclone Laboratories, Inc. Gas spargers and related container systems
US20070014187A1 (en) 2005-07-14 2007-01-18 Randy Kaas Whisk-a-bowl
WO2008040567A1 (en) 2006-10-03 2008-04-10 Artelis Flexible mixing bag, mixing device and mixing system
EP1776998B1 (en) 2005-10-21 2008-07-23 Abb Research Ltd. Mixing device
JP2009513140A (ja) 2005-10-26 2009-04-02 リーブテック，インコーポレイテッド ミキサーとスパージャーを備えたバイオリアクター
US7487688B2 (en) 2006-03-20 2009-02-10 Hyclone Laboratories, Inc. Sampling ports and related container systems
CN100496687C (zh) 2006-04-19 2009-06-10 京东方科技集团股份有限公司 颜料料浆的搅拌设备
EP2010310A4 (en) * 2006-04-21 2010-03-31 Advanced Tech Materials SUBSTANCE MIXING SYSTEMS AND DEVICES AND METHODS FOR THEIR MANUFACTURE
DE102006022306B4 (de) 2006-05-11 2009-06-25 Sartorius Stedim Biotech Gmbh Vibrationsmischer
DE202006009493U1 (de) 2006-06-14 2007-07-26 Monolith GmbH Bürosysteme Rollenschneidgerät zum Beschneiden von Blattgut
US8439554B2 (en) 2006-07-13 2013-05-14 Randy Kaas Whisk attachment
US8008065B2 (en) 2006-08-02 2011-08-30 Finesse Solutions, Llc. Disposable bioreactor vessel port
US20080116012A1 (en) * 2006-11-09 2008-05-22 Ferguson Brock E Platform Lift for a Vehicle
US20080151686A1 (en) 2006-11-14 2008-06-26 Charles Meadows Mixing bag for use with nonabrasive stir bar
USD662212S1 (en) 2007-04-10 2012-06-19 Thermotek, Inc. Butterfly wrap
WO2008151105A1 (en) 2007-06-04 2008-12-11 Ge Healthcare Bioscience Bioprocess Corp. An apparatus for mixing the contents of a container
DE102007061664B4 (de) 2007-12-18 2009-11-19 Sartorius Stedim Biotech Gmbh Behälter mit flexibler Wandung
US9044718B2 (en) 2008-03-19 2015-06-02 Sartorius Stedim Biotech Gmbh Mixing vessel
EP2279039A2 (de) 2008-04-17 2011-02-02 Sartorius Stedim Biotech GmbH Flexibler beutel mit einer mischvorrichtung
US8381780B2 (en) 2008-05-22 2013-02-26 Xcellerex, Inc. Lift and support assemblies and methods for collapsible bag containers of vessels and bioreactors
DE102008025508A1 (de) 2008-05-28 2009-12-03 Sartorius Stedim Biotech Gmbh Mischsystem
DE102008025507A1 (de) 2008-05-28 2009-12-03 Sartorius Stedim Biotech Gmbh Mischsystem
US8192071B2 (en) 2008-07-16 2012-06-05 Sartorius Stedim Biotech Gmbh Agitator apparatus with collapsible impeller
US20100105125A1 (en) 2008-10-24 2010-04-29 Bioprocessh20 Llc Systems, apparatuses and methods for cultivating microorganisms and mitigation of gases
DE102008058338B4 (de) 2008-11-20 2010-11-11 Sartorius Stedim Biotech Gmbh Rührer für Bioreaktor
EP2229853A1 (en) * 2009-03-19 2010-09-22 Green Lane Products Ltd. Milk frothing device
US8348737B2 (en) 2009-05-21 2013-01-08 Everett Page Method for conducting an online contest
JP2011023084A (ja) 2009-07-17 2011-02-03 Toshiba Corp 半導体記憶装置
KR101811114B1 (ko) 2009-10-01 2017-12-20 인터디지탈 패튼 홀딩스, 인크 전력 제어 방법 및 장치
US8506198B2 (en) 2010-02-01 2013-08-13 Hyclone Laboratories, Inc. Self aligning coupling for mixing system
US8641314B2 (en) 2010-02-01 2014-02-04 Hyclone Laboratories, Inc. Quick coupling for drive shaft
US8455242B2 (en) 2010-02-22 2013-06-04 Hyclone Laboratories, Inc. Mixing system with condenser
SE534819C2 (sv) 2010-05-06 2012-01-10 Itt Mfg Enterprises Inc Omröraraggregat för rötkammare
US8960486B2 (en) 2010-06-16 2015-02-24 Life Technologies Corporation Fluid mixing system with hangers
ES2547084T3 (es) 2010-11-30 2015-10-01 Miteco Ag Instalación y método de agitación
JP5686345B2 (ja) 2011-03-29 2015-03-18 日本電産シンポ株式会社 土練装置
AU2012308611B2 (en) 2011-09-16 2016-12-22 Global Life Sciences Solutions Usa Llc Single-use mixing and bioreactor systems
JP6101698B2 (ja) 2011-09-30 2017-03-22 ライフ テクノロジーズ コーポレイション フィルムスパージャを有する容器
EP3922712A3 (en) 2011-10-25 2022-03-09 Life Technologies Corporation Fluid mixing systems with adjustable mixing element
EP2788468A4 (en) 2011-12-09 2015-09-16 Pall Technology Uk Ltd FILTRATION DEVICE FOR PERMANENT PERFUSION
KR101949184B1 (ko) 2012-02-29 2019-02-18 도레이 카부시키가이샤 색조가 우수한 폴리아미드 수지 조성물
US9463133B2 (en) 2012-04-02 2016-10-11 Maverick Sports Medicine, Inc. Deep tissue massage roller
WO2013151733A1 (en) 2012-04-06 2013-10-10 Hyclone Laboratories, Inc. Fluid mixing system with flexible drive line and foldable impeller
CN202606066U (zh) 2012-04-25 2012-12-19 林美复合材料(苏州)有限公司 一种搅拌机
CA2993564C (en) 2013-04-19 2019-10-22 Emd Millipore Corporation Flexible film baffle in single use bioreactor
CA2922967C (en) 2013-09-16 2022-05-03 Genentech, Inc. Bioreactors with multiple or adjustable-position agitator designs
WO2016065249A1 (en) * 2014-10-24 2016-04-28 Life Technologies Corporation Acoustically settled liquid-liquid sample purification system
EP3259342A4 (en) 2015-02-17 2018-10-17 Genea IP Holdings Pty Limited Method and apparatus for dynamically culturing a biological sample
US9714405B2 (en) * 2015-04-14 2017-07-25 Pall Corporation Bioprocessing container tube system and method of use
EP3283207B1 (en) * 2015-04-16 2024-01-31 Global Life Sciences Solutions USA LLC Bioprocess mixer
KR102485147B1 (ko) 2015-07-06 2023-01-06 엘지전자 주식회사 변형 가능한 디스플레이 장치 및 그의 동작 방법
US9683208B2 (en) * 2015-07-31 2017-06-20 Ernest Louis Stadler Horizontal single use pressurizable modular multi-agitator microbial fermentator
US10836989B2 (en) * 2015-10-16 2020-11-17 Global Life Sciences Solutions Usa Llc Disposable container, mixing system and packaging
USD830544S1 (en) 2016-05-17 2018-10-09 Detectachem Llc Receptacle
USD857188S1 (en) 2016-09-29 2019-08-20 Mine Survival, Inc. Rebreather device
USD824042S1 (en) 2016-11-10 2018-07-24 Viacyte, Inc. Perforated cell encapsulation device
USD828653S1 (en) 2016-12-14 2018-09-11 Brandon Penland Treatment applicator
US11788048B2 (en) * 2017-09-19 2023-10-17 Life Technologies Corporation Systems and methods for a collapsible chamber with foldable mixing element
USD870315S1 (en) 2017-11-09 2019-12-17 Hemocue Ab Cuvette
EP3740559A1 (en) * 2018-01-17 2020-11-25 Life Technologies Corporation System and method for cell culture scaling
US20230150756A1 (en) * 2021-11-17 2023-05-18 Coldsnap, Corp. Rapidly Cooling Food and Drinks

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102112595A (zh) * 2008-08-06 2011-06-29 普莱克斯技术有限公司 用于控制哺乳动物细胞培养过程的系统和方法
CN102307984A (zh) * 2009-02-09 2012-01-04 英国龙沙生物医药股份有限公司 用于培养哺乳动物细胞的生物反应器
US20130288346A1 (en) * 2011-01-11 2013-10-31 Xcellerex Inc. Linearly scalable single use bioreactor system
US20150118753A1 (en) * 2012-04-18 2015-04-30 Life Technologies Corporation Methods and apparatus for gas stream mass transfer with a liquid
US20170183617A1 (en) * 2015-12-29 2017-06-29 Life Technologies Corporation Fluid mixing system with laterally displaced flexible drive lines and methods of use
US20170349874A1 (en) * 2016-06-03 2017-12-07 Lonza Ltd Single Use Bioreactor
US20180010082A1 (en) * 2016-06-03 2018-01-11 Lonza Ltd Bioreactor With Higher Agitation Rates

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112246173A (zh) * 2020-09-29 2021-01-22 广州市爱百伊生物技术有限公司 一种配料罐及生产线
TWI822173B (zh) * 2021-08-17 2023-11-11 日商日揮股份有限公司 運算裝置、控制裝置、培養系統以及培養系統的設計方法

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