CN111678238A

CN111678238A - Dual-purpose PCR laboratory system and operation method thereof

Info

Publication number: CN111678238A
Application number: CN202010696435.XA
Authority: CN
Inventors: 黄清; 李蓉; 曾黎峰; 徐勇飞; 夏晓健; 万本愿
Original assignee: Jiangxi Provincial Peoples Hospital
Current assignee: Jiangxi Provincial Peoples Hospital
Priority date: 2020-07-20
Filing date: 2020-07-20
Publication date: 2020-09-18

Abstract

The invention discloses a dual-purpose PCR laboratory system and an operation method thereof, belonging to the field of PCR laboratories, wherein the dual-purpose PCR laboratory system comprises an entry area, a special PCR passage, a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area, wherein the reagent preparation area comprises a reagent preparation area independent exhaust device and a reagent preparation area independent air supply device, the sample preparation area comprises a sample preparation area independent exhaust device and a sample preparation area independent air supply device, the gene amplification area comprises a gene amplification chamber airtight window, a gene amplification area independent exhaust device and a gene amplification area independent air supply device, and the product analysis area comprises a product analysis chamber airtight window, a product analysis area independent exhaust device and a product analysis area independent air supply device. The dual-purpose PCR laboratory system disclosed by the invention realizes relatively independent ventilation among all the zones, does not have the condition of cross contamination, is applicable to both a normal state and an epidemic situation state, and has extremely high safety performance.

Description

Dual-purpose PCR laboratory system and operation method thereof

Technical Field

The invention relates to the field of PCR laboratories, in particular to a dual-purpose PCR laboratory system and an operation method thereof.

Background

The PCR laboratory is also called a gene amplification laboratory, and the PCR laboratory generally comprises a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area. PCR is a short term for Polymerase Chain Reaction (Polymerase Chain Reaction), a molecular biology technique used to amplify specific DNA fragments and can be used as a special DNA copy in vitro. The virus content in the body of a patient can be rapidly mastered by a DNA gene tracking system, and the accuracy of the system reaches the nanometer level. For example, the number of hepatitis B viruses in a patient body, whether the hepatitis B viruses are duplicated or not, whether the hepatitis B viruses are infected or not, how strong the infection exists, whether the hepatitis B viruses need to be taken or not, whether the liver functions are abnormally changed or not can be accurately detected, which antiviral drugs are most suitable for the patient to use can be judged in time, and the curative effect of the drugs can be judged, so that a reliable inspection basis is provided for clinical treatment.

In the process of implementing the embodiment of the invention, the inventor finds that at least the following defects exist in the background art:

each district of PCR laboratory all need ventilate, and in the current PCR laboratory, it all adopts the mode of circulation ventilation, and adopt the huge conventional purification unit of size to send out and arrange, be about to in the air discharge back in each district gets into each district once more through purification unit etc. with reuse air temperature and energy-conservation, however, circulation ventilation leads to the air current can't absolutely keep apart, easily make air cross contamination, not only lead to the air of pollution and circulate in each interval of PCR laboratory, seriously threaten medical science research and inspector's health, the security performance is low, and can pollute test material such as reagent, sample, lead to the testing result to be out of alignment. Especially, in the period of resisting epidemic situations such as Severe Acute Respiratory Syndrome (SARS), avian influenza infection of people, Middle East Respiratory Syndrome (MERS), novel coronavirus pneumonia (COVID-19) and the like, the problems are aggravated due to strong infectivity, and the common PCR laboratory is disabled.

Disclosure of Invention

The embodiment of the application provides a dual-purpose PCR laboratory system and an operation method thereof, solves the technical problems that in the prior art, the health of medical research and inspection personnel is easily threatened and the detection result is inaccurate because air cannot be absolutely isolated among all the areas of a PCR laboratory, and the PCR laboratory fails when encountering an epidemic situation, realizes relatively independent ventilation among all the areas of the PCR laboratory, does not have the condition of cross contamination, is applicable to both a normal state and an epidemic situation, and has high safety performance.

In one aspect, embodiments of the present application provide a dual-purpose PCR laboratory system, including:

an entry zone, the entry zone comprising: a clean room; the preparation room is communicated with the clean room through a door body; the dressing room is communicated with the preparation room through a door body;

the special PCR walkway is communicated with the dressing room through a door body;

a reagent preparation zone comprising: the reagent preparation buffer room is communicated with the PCR special passage through a door body; the reagent preparation room is communicated with the reagent preparation buffer room through a door body and is configured to prepare a stored reagent, split-package the reagent and prepare a main reaction mixed solution; the reagent preparation chamber air-tight window is arranged on one side wall of the reagent preparation chamber far away from the reagent preparation buffer chamber in an opening and closing manner; a reagent preparation zone independent air exhaust device configured to exhaust air in the reagent preparation compartment to an outside of the reagent preparation compartment; a reagent preparation zone independent air supply device configured to deliver air into the reagent preparation compartment;

a sample preparation zone, the sample preparation zone comprising: the sample preparation buffer room is communicated with the PCR special passage through a door body; the sample preparation room is communicated with the sample preparation buffer room through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into the amplification reaction tube and synthesize cDNA; a sample preparation room airtight window which is openably and closably arranged on one side wall of the sample preparation room far away from the sample preparation buffer room; a sample preparation zone independent exhaust device configured to exhaust air in the sample preparation compartment to an exterior of the sample preparation compartment; a sample preparation zone independent air supply device configured to deliver air into the sample preparation compartment;

a gene amplification region, said gene amplification region comprising: the gene amplification buffer room is communicated with the PCR special walkway through a door body; a gene amplification chamber, wherein the gene amplification chamber is communicated with the gene amplification buffer chamber through a door body, and the gene amplification chamber is configured to amplify DNA or cDNA; the gene amplification chamber airtight window is arranged on one side wall of the gene amplification chamber far away from the gene amplification buffer chamber in an opening and closing manner; a gene amplification region independent air exhaust device configured to exhaust air in the gene amplification chamber to the outside of the gene amplification chamber; a gene amplification region independent air supply device configured to supply air into the gene amplification chamber; and

a product analysis zone, the product analysis zone comprising: a product analysis buffer room communicated with the PCR special passage through a door body; a product analysis room, wherein the product analysis room is communicated with the product analysis buffer room through a door body, and the product analysis room is configured to measure the amplified fragments; a product analysis chamber gas seal, the product analysis chamber gas seal being openably and closably disposed on a sidewall of the product analysis chamber remote from the product analysis buffer chamber; a product analysis zone independent exhaust configured to exhaust air in the product analysis compartment to an exterior of the product analysis compartment; a product analysis zone independent air supply device configured to supply air into the product analysis compartment;

wherein the independent exhaust device of the reagent preparation area, the independent exhaust device of the sample preparation area, the independent exhaust device of the gene amplification area and the independent exhaust device of the product analysis area are independent and not communicated with each other, and the independent exhaust device of the reagent preparation area and the independent air supply device of the reagent preparation area are independent and not communicated with each other, the independent exhaust device of the sample preparation area and the independent air supply device of the sample preparation area are independent and not communicated with each other, the gene amplification area independent air exhaust device and the gene amplification area independent air supply device are independent and not communicated with each other, the independent exhaust device of the product analysis area and the independent air supply device of the product analysis area are independent and not communicated with each other, to prevent contamination between the reagent preparation chambers, the sample preparation chambers, the gene amplification chambers, and the product analysis chambers;

the dual-purpose PCR laboratory system comprises a first state and a second state, wherein in the first state, the reagent preparation room airtight window, the sample preparation room airtight window, the gene amplification room airtight window and the product analysis room airtight window are all opened so as to enable the reagent preparation room, the sample preparation room, the gene amplification room and the product analysis room to be respectively communicated with the outside, and at least one of the reagent preparation area independent air supply device, the sample preparation area independent air supply device, the gene amplification area independent air supply device and the product analysis area independent air supply device is in a working state or a stop state; in the second state, the reagent preparation room airtight window, the sample preparation room airtight window, the gene amplification room airtight window and the product analysis room airtight window are all closed, so that the reagent preparation room, the sample preparation room, the gene amplification room and the product analysis room are respectively in a closed state, and the reagent preparation area independent air exhaust device, the sample preparation area independent air exhaust device, the gene amplification area independent air exhaust device, the product analysis area independent air exhaust device, the reagent preparation area independent air supply device, the sample preparation area independent air supply device, the gene amplification area independent air supply device and the product analysis area independent air supply device are all in a working state.

In another aspect, the present application provides a method for operating a dual-purpose PCR laboratory system, which is suitable for the dual-purpose PCR laboratory system, and the method includes:

when a conventional PCR project needs to be carried out, the dual-purpose PCR laboratory system is operated in a first state, so that the airtight window of the reagent preparation room, the airtight window of the sample preparation room, the airtight window of the gene amplification room and the airtight window of the product analysis room are all opened, the reagent preparation room, the sample preparation room, the gene amplification room and the product analysis room are respectively communicated with the outside to naturally ventilate, and the independent air supply device of the reagent preparation area, the independent air supply device of the sample preparation area, the independent air supply device of the gene amplification area and the independent air supply device of the product analysis area are all in a stop state, and the independent air exhaust device of the reagent preparation area, the independent air exhaust device of the sample preparation area, the independent air exhaust device of the gene amplification area and the independent air exhaust device of the product analysis area are all in a working state;

when an epidemic situation PCR project needs to be carried out, the dual-purpose PCR laboratory system is operated in the second state, the airtight window of the reagent preparation room, the airtight window of the sample preparation room, the airtight window of the gene amplification room and the airtight window of the product analysis room are all closed, so that the reagent preparation room, the sample preparation room, the gene amplification room and the product analysis room are respectively in the airtight state, the independent air supply device of the reagent preparation area, the independent air supply device of the sample preparation area, the independent air supply device of the gene amplification area and the independent air supply device of the product analysis area are all in the working state, and the independent air exhaust device of the reagent preparation area, the independent air exhaust device of the sample preparation area, the independent air exhaust device of the gene amplification area and the independent air exhaust device of the product analysis area are all in the working state.

One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

the dual-purpose PCR laboratory system and the operation method thereof provided by the embodiment of the application are characterized in that an entry area, a special PCR channel, a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area are arranged, the reagent preparation area comprises a reagent preparation buffer room, a reagent preparation room, an airtight window of the reagent preparation room, an independent exhaust device of the reagent preparation area and an independent air supply device of the reagent preparation area, the sample preparation area comprises a sample preparation buffer room, a sample preparation room, an airtight window of the sample preparation room, an independent exhaust device of the sample preparation area and an independent air supply device of the sample preparation area, the gene amplification area comprises a gene amplification buffer room, a gene amplification room, an airtight window of the gene amplification room, an independent exhaust device of the gene amplification area and an independent air supply device of the gene amplification area, the product analysis area comprises a product analysis buffer room, a product analysis room and an airtight window of the product analysis room, The independent air exhaust device of the reagent preparation area, the independent air exhaust device of the sample preparation area, the independent air exhaust device of the gene amplification area and the independent air exhaust device of the product analysis area are independent of each other and are not communicated with each other, the independent air exhaust device of the reagent preparation area and the independent air supply device of the reagent preparation area are independent of each other and are not communicated with each other, the independent air exhaust device of the sample preparation area and the independent air supply device of the sample preparation area are independent of each other and are not communicated with each other, the independent air exhaust device of the gene amplification area and the independent air supply device of the gene amplification area are independent of each other and are not communicated with each other, the independent air supply device of the reagent preparation area supplies air into the reagent preparation room independently, and the independent air in the reagent preparation room is discharged independently through the independent air exhaust device of the reagent preparation area, independently supplying air into the sample preparation room through the independent air supply device of the sample preparation area, independently discharging air in the sample preparation room through the independent air exhaust device of the sample preparation area, independently supplying air into the gene amplification room through the independent air supply device of the gene amplification area, independently discharging air in the gene amplification room through the independent air exhaust device of the gene amplification area, independently supplying air into the product analysis room through the independent air supply device of the product analysis area, and independently discharging air in the product analysis room through the independent air exhaust device of the product analysis area, so that the reagent preparation area, the sample preparation area, the gene amplification area and the product analysis area can independently supply air and independently discharge air, effectively prevent air cross contamination, ensure the ventilation of each area to be independent, effectively prevent air cross or instantaneously generated positive pressure backflow in emergency, the health of medical research and inspection personnel is guaranteed, the safety performance is effectively improved, test substances such as reagents and specimens can be effectively prevented from being polluted, and the accuracy of a detection result is guaranteed.

Meanwhile, because the dual-purpose PCR laboratory system is arranged to have a first state and a second state, when a conventional PCR project is carried out, the dual-purpose PCR laboratory system can be operated under the first state, so that the airtight window of the reagent preparation room, the airtight window of the sample preparation room, the airtight window of the gene amplification room and the airtight window of the product analysis room are all opened, so that the reagent preparation room, the sample preparation room, the gene amplification room and the product analysis room are respectively communicated with the outside and naturally ventilate, the independent air supply device of the reagent preparation area, the independent air supply device of the sample preparation area, the independent air supply device of the gene amplification area and the independent air supply device of the product analysis area are all closed and in a stop state, the independent air exhaust device of the reagent preparation area, the independent air exhaust device of the sample preparation area, the independent air exhaust device of the gene amplification area and the independent air exhaust device of the product analysis area are all in an open state, good independent ventilation can be ensured, the air exhaust device is opened, energy is saved, and the purification air conditioning unit is arranged without wasting space. When encountering Severe Acute Respiratory Syndrome (SARS), human infection avian influenza, Middle East Respiratory Syndrome (MERS), novel coronavirus pneumonia (COVID-19) and other epidemic situations, the device can operate in a second state, so that the airtight window of the reagent preparation room, the airtight window of the sample preparation room, the airtight window of the gene amplification room and the airtight window of the product analysis room are all closed, so that the reagent preparation room, the sample preparation room, the gene amplification room and the product analysis room are respectively sealed and isolated, and simultaneously, the independent air supply device of the reagent preparation area, the independent air supply device of the sample preparation area, the independent air supply device of the gene amplification area, the independent air supply device of the product analysis area, the independent air exhaust device of the reagent preparation area, the independent air exhaust device of the sample preparation area, the independent air supply device of the gene amplification area and the independent air exhaust device of the product analysis area are simultaneously opened, so that the air supply and the air exhaust of each area are respectively and independently carried out, thereby, is extremely suitable for epidemic PCR projects.

From the above, the dual-purpose PCR laboratory system and the operation method thereof provided in the embodiments of the present application effectively solve the technical problems that the medical research and the health of the inspector are easily threatened and the detection result is inaccurate due to the fact that air cannot be absolutely isolated between the regions of the PCR laboratory in the prior art, and the PCR laboratory fails when encountering an epidemic situation, realize relatively independent ventilation between the regions of the PCR laboratory, do not have the condition of cross contamination, have high safety performance, and are applicable both in a normal state and an epidemic situation, and do not need to temporarily build or modify the PCR laboratory when encountering an epidemic situation, thereby effectively ensuring the instant use under the special conditions such as an epidemic situation, realizing the universality between a normal condition and a special condition by once building, and greatly saving the cost and time of new building or modifying.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.

In the drawings:

FIG. 1 is a plan view of a dual-purpose PCR laboratory system provided in an embodiment of the present application;

FIG. 2 is a schematic diagram of a dual-purpose PCR laboratory system according to an embodiment of the present disclosure;

FIG. 3 is a schematic diagram of a reagent preparation area provided in an embodiment of the present application;

FIG. 4 is a schematic structural diagram of a sample preparation area provided in an embodiment of the present application;

FIG. 5 is a schematic diagram showing the structure of a gene amplification region provided in the examples of the present application;

FIG. 6 is a schematic diagram of the structure of a product analysis region provided in an embodiment of the present application;

FIG. 7 is a schematic structural view of a gas-tight window of a reagent preparation room provided in an embodiment of the present application;

FIG. 8 is a schematic diagram of the dual-purpose PCR laboratory system provided in example 2 of the present application;

FIG. 9 is a schematic diagram of the dual-purpose PCR laboratory system provided in example 3 of the present application.

In the figure:

100. a dual-purpose PCR laboratory system; 10. entering a zone; 11. a clean room; 12. a preparation room; 13. a dressing room; 20. a PCR dedicated walkway; 30. a reagent preparation zone; 31. a reagent preparation buffer chamber; 32. a reagent preparation room; 33. a reagent preparation chamber airtight window; 34. the reagent preparation area is provided with an independent air exhaust device; 35. the reagent preparation area is provided with an independent air supply device; 40. a sample preparation zone; 41. a sample preparation buffer room; 42. a sample preparation room; 43. a sample preparation room airtight window; 44. the sample preparation area is provided with an independent air exhaust device; 45. the sample preparation area independent air supply device; 50. a gene amplification region; 51. a gene amplification buffer; 52. during gene amplification; 53. a gene amplification chamber airtight window; 54. an independent exhaust device in the gene amplification area; 55. the gene amplification area independent air supply device; 60. a product analysis zone; 61. a product analysis buffer; 62. a product analysis room; 63. a product analysis chamber gas tight window; 64. an independent exhaust device in the product analysis area; 65. the product analysis area is provided with an independent air supply device; 341. the reagent preparation area is provided with an independent air exhaust main pipeline; 342. the reagent preparation area is provided with an independent exhaust fan; 343. the reagent preparation area air exhaust high-efficiency filter; 344. preparing an exhaust branch pipe of the buffer room by using the reagent; 345. an exhaust branch pipe of the reagent preparation room; 346. an air exhaust regulating valve of the reagent preparation buffer room; 347. an air exhaust regulating valve of the reagent preparation room; 348. a main air exhaust regulating valve in the reagent preparation area; 349. the reagent preparation area air exhaust check valve; 441. the sample preparation area is provided with an independent air exhaust main pipeline; 442. the sample preparation area is provided with an independent exhaust fan; 443. a sample preparation area air exhaust high-efficiency filter; 444. an exhaust branch pipe of the sample preparation buffer room; 445. an exhaust branch pipe of the sample preparation room; 446. an air exhaust regulating valve of the sample preparation buffer room; 447. an air exhaust adjusting valve of the sample preparation room; 448. a sample preparation area air exhaust main regulating valve; 449. a sample preparation area air exhaust check valve; 541. the gene amplification area is an independent air exhaust main pipeline; 542. a gene amplification region independent exhaust fan; 543. a high-efficiency exhaust filter in the gene amplification area; 544. an exhaust branch pipe of the gene amplification buffer room; 545. exhaust branch pipes between gene amplification; 546. an exhaust regulating valve of the gene amplification buffer room; 547. an air exhaust regulating valve between gene amplification; 548. a main air exhaust regulating valve in the gene amplification region; 549. an air exhaust check valve in the gene amplification area; 641. the product analysis area is provided with an independent air exhaust main pipeline; 642. an independent exhaust fan in the product analysis area; 643. a high-efficiency exhaust filter in the product analysis area; 644. product analysis buffer room exhaust branch pipe; 645. exhaust branch pipes of the product analysis room; 646. an exhaust regulating valve of the product analysis buffer room; 647. an exhaust regulating valve of the product analysis room; 648. a main exhaust regulating valve in the product analysis area; 649. an exhaust check valve in the product analysis area; 351. the reagent preparation area is provided with an independent air supply main pipeline; 352. reagent preparation buffers the air supply branch pipe of room; 353. the reagent preparation area is provided with an independent blower; 354. a reagent preparation area air supply filter; 355. the reagent preparation buffer room air supply regulating valve; 356. an air supply adjusting valve of the reagent preparation room; 357. a first main regulating valve for air supply of the reagent preparation area; 358. a second main regulating valve for air supply of the reagent preparation area; 451. the sample preparation area is provided with an independent air supply main pipeline; 452. a sample preparation buffer room air supply branch pipe; 453. a sample preparation area independent blower; 454. a sample preparation area air supply filter; 455. an air supply adjusting valve of the sample preparation buffer room; 456. an air supply adjusting valve of the sample preparation room; 457. a first main adjusting valve is supplied to the sample preparation area; 458. a second main adjusting valve is supplied to the sample preparation area; 551. the gene amplification area independently supplies air to the main pipeline; 552. a gene amplification buffer room air supply branch pipe; 553. the gene amplification region is provided with an independent blower; 554. a gene amplification region air supply filter; 555. an air supply regulating valve between the gene amplification buffers; 556. an air supply regulating valve between gene amplification; 557. a first main regulating valve is blown in the gene amplification area; 558. a second main regulating valve is blown in the gene amplification area; 651. a main pipeline for independently supplying air to the product analysis area; 652. a product analysis buffer room air supply branch pipe; 653. a product analysis zone independent blower; 654. a product analysis area air supply filter; 655. a product analysis buffer room air supply regulating valve; 656. an air supply regulating valve of the product analysis room; 657. a first main regulating valve is supplied to the product analysis area; 658. a second main regulating valve is supplied to the product analysis area; 131. a first dressing room; 132. a wind showering room; 133. a second dressing room; 134. a first sealing door; 135. a second sealing door; 136. a third sealing door; 137. a fourth sealing door; 138. a fifth sealing door; 359. a PCR special walkway air supply branch pipe; 350. a PCR special walkway air supply regulating valve; 4401. a PCR special walkway exhaust branch pipe; 4402. the PCR special walkway air exhaust regulating valve; 401. the first sample preparation area is provided with an independent air exhaust main pipeline; 402. the second sample preparation area is provided with an independent air exhaust main pipeline; 403. the first sample preparation area is provided with an independent exhaust fan; 404. a second sample preparation area independent exhaust fan; 405. a second sample preparation buffer room exhaust branch pipe; 406. a second PCR special walkway exhaust branch pipe; 407. a first sample preparation area air exhaust high-efficiency filter; 408. a second sample preparation area air exhaust high-efficiency filter; 4501. the first sample preparation area is provided with an independent air supply main pipeline; 4502. the second sample preparation area is provided with an independent air supply main pipeline; 4503. the first sample preparation area is provided with an independent blower; 4504. a second sample preparation area independent blower; 4505. and a second sample preparation buffer room air supply branch pipe.

Detailed Description

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

It is noted that, unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

In the present invention, unless stated to the contrary, use of the directional terms "upper and lower" are generally directed to the orientation shown in the drawings, or to the vertical, or gravitational direction; likewise, for ease of understanding and description, "left and right" are generally to the left and right as shown in the drawings; "inner and outer" refer to the inner and outer relative to the profile of the respective member itself, but the above directional terms are not intended to limit the present invention.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In order to solve the technical problems that the health of medical research and inspection personnel is threatened and the detection result is inaccurate because the areas of the PCR laboratory cannot be mutually independent and absolutely isolate air in the prior art, and the technical problem that the PCR laboratory fails when encountering epidemic situations, the embodiment of the application provides a dual-purpose PCR laboratory system and an operation method thereof.

The invention is further described below with reference to the accompanying drawings.

Example 1

As shown in fig. 1 to 7, the present embodiment provides a dual-purpose PCR laboratory system 100, and the dual-purpose PCR laboratory system 100 includes an entry area 10, a PCR-dedicated lane 20, a reagent preparation area 30, a sample preparation area 40, a gene amplification area 50, and a product analysis area 60.

The access area 10 includes a clean room 11, a preparation room 12, and a dressing room 13. The preparation room 12 is communicated with the clean room 11 through a door body, and the dressing room 13 is communicated with the preparation room 12 through a door body. The clean room 11 is provided with an entrance door for medical research and inspection personnel to enter, and then sequentially passes through the clean room 11, the preparation room 12 and the dressing room 13 to enter the PCR passage 20.

The PCR dedicated passage 20 is communicated with the dressing room 13 through a door body, and the PCR dedicated passage 20 is a common corridor which enters the reagent preparation area 30, the sample preparation area 40, the gene amplification area 50 and the product analysis area 60. The air pressure of the PCR exclusive path 20 may be +10 PA.

The reagent preparation area 30 comprises a reagent preparation buffer room 31, a reagent preparation room 32, a reagent preparation room airtight window 33, a reagent preparation area independent air exhaust device 34 and a reagent preparation area independent air supply device 35.

The reagent preparation buffer room 31 is communicated with the special PCR passage 20 through a door body, and the reagent preparation buffer room 31 is a buffer space entering the reagent preparation room 32 from the special PCR passage 20. The air pressure of the reagent preparation buffer compartment 31 may be 0 PA.

The reagent preparation room 32 is communicated with the reagent preparation buffer room 31 through a door body, and the reagent preparation room 32 is configured to prepare a stored reagent, split the reagent and prepare a main reaction mixed solution. The reagents and materials for specimen preparation are directly transported to the reagent preparing room 32, and the reagent raw materials are stored in the reagent preparing room 32. The reagent preparation room 32 can be equipped with a 2-8 ℃ refrigerator and a-80 ℃ refrigerator. The area of the room may be 15m²～20m². The pressure gradient of the reagent preparation chamber 32 may be in a relatively positive pressure state (may be +10PA) to prevent the outside air containing nucleic acid aerosol from entering and causing pollution. A reagent preparation room air conditioner is provided in the reagent preparation room 32, and is configured to adjust the temperature and humidity inside the reagent preparation room.

The reagent preparation room air-tight window 33 is openably and closably disposed on a side wall of the reagent preparation room 32 away from the reagent preparation buffer room 31, the reagent preparation room 32 can naturally ventilate with the outside when the reagent preparation room air-tight window 33 is opened, and the reagent preparation room 32 can be in a closed state when the reagent preparation room air-tight window 33 is closed, thereby preventing the outside air from entering.

The reagent preparing zone independent air discharging device 34 is configured to discharge air in the reagent preparing compartment 32 to the outside of the reagent preparing compartment 32.

The reagent preparation area independent air blowing device 35 is configured to blow air into the reagent preparation compartment 32.

The sample preparation area 40 includes a sample preparation buffer room 41, a sample preparation room 42, a sample preparation room airtight window 43, a sample preparation area independent air exhaust device 44, and a sample preparation area independent air supply device 45.

The sample preparation buffer room 41 is communicated with the PCR special passage 20 through a door body. The sample preparation buffer room 41 is a buffer space that enters the sample preparation room 42 from the PCR-dedicated lane 20. The air pressure of the sample preparation buffer 41 may be 0 PA.

The sample preparation room 42 is communicated with the sample preparation buffer room 41 through a door body, and the sample preparation room 42 is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA. The sample preparation room 42 may be equipped with a 2-8 ℃ refrigerator and a-20 ℃ refrigerator. The sample preparation room 42 is equipped with a biosafety cabinet for performing operations for extracting nucleic acids. To avoid repeated cycling of the extracted nucleic acids within the cabinet, resulting in cross-contamination between samples, and false positive results, the sample preparation booth 42 must be equipped with a biosafety cabinet of type B2. The vertical air flow of the working area of the biological safety cabinet comes from a laboratory, and the exhausted air is directly exhausted to the outside after being filtered by the high-efficiency filter and is not allowed to return to the safety cabinet and the laboratory. The area of the sample preparation compartment 42 may be 25m²～30m²In the meantime. The pressure gradient in the sample preparation bay 42 is desirably positive with respect to the adjacent area (which may be-10 PA) to avoid aerosol contamination from the adjacent area into the local area. A sample preparation room air conditioner is disposed in the sample preparation room 42 and configured to adjust the temperature and humidity inside the sample preparation room.

The sample preparation chamber air-tight window 43 is openably and closably disposed on a side wall of the sample preparation chamber 42 away from the sample preparation buffer chamber 41. When the air-tight window 43 of the sample preparation room is opened, the sample preparation room 42 can naturally ventilate with the outside, and when the air-tight window 43 of the sample preparation room is closed, the sample preparation room 42 can be in a closed state, so that the outside air is prevented from entering.

The sample preparation zone independent exhaust device 44 is configured to exhaust air in the sample preparation compartment 42 to the outside of the sample preparation compartment 42.

The sample preparation section independent air blowing device 45 is configured to blow air into the sample preparation compartment 42.

The gene amplification region 50 includes a gene amplification buffer room 51, a gene amplification room 52, a gene amplification room airtight window 53, a gene amplification region independent air exhaust device 54, and a gene amplification region independent air supply device 55.

The gene amplification buffer room 51 is communicated with the PCR special passage 20 through a door body. The gene amplification buffer room 51 is a buffer space which enters the gene amplification room 52 from the PCR dedicated path 20. The gas pressure in the gene amplification buffer 51 may be 0 PA.

The gene amplification room 52 communicates with the gene amplification buffer room 51 through a gate body, and the gene amplification room 52 is configured to amplify DNA or cDNA. In addition, addition of the prepared DNA template and synthesized cDNA (from the sample preparation room 42), preparation of the main reaction mixture (from the reagent preparation room 32) into a reaction mixture, and the like may be performed in the gene amplification room 52. In nested PCR assays, the reaction tube must usually be opened after the first round of amplification, so nested amplification has a high risk of contamination, and the second sample addition must be performed in the gene amplification chamber 52. The gene amplification room 52 is equipped with the core instrument of the PCR laboratory: as the PCR instrument, for example, two types of PCR instruments, ABI7500 and ABI9700, can be used. The area of the gene amplification chamber 52 can be controlled to be 15m²～20m². The pressure gradient of the gene amplification chamber 52 is required to be negative pressure with respect to the adjacent region (the gene amplification chamber 52 may be-15 PA) to avoid the escape of aerosol from the region. To avoid contamination by aerosols, unnecessary movement within the region should be minimized. The gene amplification room 52 is provided with a gene amplification room air conditioner configured to regulate the temperature and humidity inside the gene amplification room 52.

The airtight window 53 is openably and closably provided on a side wall of the gene amplification chamber 52 remote from the gene amplification buffer chamber 51. When the airtight window 53 of the gene amplification chamber is opened, the gene amplification chamber 52 can naturally ventilate with the outside, and when the airtight window 53 of the gene amplification chamber is closed, the gene amplification chamber 52 can be in a closed state, so that the outside air is prevented from entering.

The gene amplification region independent air exhausting device 54 is configured to exhaust the air in the gene amplification chamber 52 to the outside of the gene amplification chamber 52.

The gene amplification region independent air blowing device 55 is arranged to blow air into the gene amplification chamber 52.

Product analysis area 60 includes product analysis buffer room 61, product analysis room 62, product analysis room air-tight window 63, product analysis area independent air exhaust device 64, and product analysis area independent air supply device 65.

The product analysis buffer room 61 is communicated with the PCR special passage 20 through a door body. The product analysis buffer room 61 is a buffer channel from the PCR dedicated walkway 20 into the product analysis room 62. The pressure in product analysis buffer 61 may be 0 PA.

The product analysis room 62 communicates with the product analysis buffer room 61 through a door, and the product analysis room 62 is configured to measure the amplified fragment. The area of the product analysis chamber 62 can be controlled to be 15m²～20m². The product analysis chamber 62 is the most important source of contamination of the amplification product, and therefore the pressure gradient in this region is required to be negative with respect to the adjacent region (the product analysis chamber 62 may be-20 PA) to prevent diffusion of the amplification product from this region to other regions. A product analysis room air conditioner is provided in the product analysis room 62, and is configured to adjust the temperature and humidity inside the product analysis room 62.

The product analysis chamber gas-tight window 63 is openably and closably disposed on a sidewall of the product analysis chamber 62 away from the product analysis buffer chamber 61. When the product analysis chamber airtight window 63 is opened, the product analysis chamber 62 can naturally ventilate with the outside, and when the product analysis chamber airtight window 63 is closed, the product analysis chamber 62 can be in a closed state, so that the outside air is prevented from entering.

Product analysis zone independent exhaust 64 is configured to exhaust air in product analysis compartment 62 to the exterior of product analysis compartment 62.

The product analysis zone independent air blowing device 65 is configured to blow air into the product analysis compartment 62.

The reagent preparation area independent exhaust device 34, the sample preparation area independent exhaust device 44, the gene amplification area independent exhaust device 54 and the product analysis area independent exhaust device 64 are independent of each other and are not communicated with each other, the reagent preparation area independent exhaust device 34 and the reagent preparation area independent air supply device 35 are independent of each other and are not communicated with each other, the sample preparation area independent exhaust device 44 and the sample preparation area independent air supply device 45 are independent of each other and are not communicated with each other, the gene amplification area independent exhaust device 54 and the gene amplification area independent air supply device 55 are independent of each other and are not communicated with each other, the product analysis area independent exhaust device 64 and the product analysis area independent air supply device 65 are independent of each other and are not communicated with each other, so that the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52 and the product analysis room 62 are prevented from being polluted.

The dual-purpose PCR laboratory system comprises a first state and a second state, wherein in the first state, the reagent preparation room airtight window 33, the sample preparation room airtight window 43, the gene amplification room airtight window 53 and the product analysis room airtight window 63 are all opened so as to enable the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52 and the product analysis room 62 to be respectively communicated with the outside, and at least one of the reagent preparation area independent air supply device 35, the sample preparation area independent air supply device 45, the gene amplification area independent air supply device 55 and the product analysis area independent air supply device 65 is in a working state or a stop state; in the second state, the reagent preparation room air-tight window 33, the sample preparation room air-tight window 43, the gene amplification room air-tight window 53, and the product analysis room air-tight window 63 are all closed, so that the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52, and the product analysis room 62 are respectively in a closed state, and the reagent preparation area independent air exhaust device 34, the sample preparation area independent air exhaust device 44, the gene amplification area independent air exhaust device 54, the product analysis area independent air exhaust device 64, the reagent preparation area independent air supply device 35, the sample preparation area independent air supply device 45, the gene amplification area independent air supply device 55, and the product analysis area independent air supply device 65 are all in a working state.

In the dual-purpose PCR laboratory system according to the embodiment of the present application, the independent reagent preparation area air supply device 35 independently supplies air into the reagent preparation chamber 32, the independent reagent preparation area air exhaust device 34 independently exhausts air from the reagent preparation chamber 32, the independent sample preparation area air supply device 45 independently supplies air into the sample preparation chamber 42, the independent sample preparation area air exhaust device 44 independently exhausts air from the sample preparation chamber 42, the independent gene amplification area air supply device 55 independently supplies air into the gene amplification chamber 52, the independent gene amplification area air exhaust device 54 independently exhausts air from the gene amplification chamber 52, the independent product analysis area air supply device 65 independently supplies air into the product analysis chamber 62, and the independent product analysis area air exhaust device 64 independently exhausts air from the product analysis chamber 62, so that the reagent preparation area 30, and the sample preparation area 42 are independently controlled by the independent reagent preparation area air supply device 35, and the air in the reagent preparation chamber 32 is independently exhausted by the independent gene amplification area air exhaust device 64, The sample preparation area 40, the gene amplification area 50 and the product analysis area 60 can independently intake air and independently exhaust air, so that cross air pollution can be effectively prevented, each area can be independently ventilated under the condition of ensuring smooth ventilation of each area, the health of medical research and inspection personnel is ensured, the safety performance is effectively improved, test substances such as reagents and specimens can be effectively prevented from being polluted, and the accuracy of a detection result is ensured.

Meanwhile, since the dual-purpose PCR laboratory system is provided with a first state and a second state, when a conventional PCR project is carried out, the system can be operated in the first state, so that the reagent preparation chamber airtight window 33, the sample preparation chamber airtight window 43, the gene amplification chamber airtight window 53 and the product analysis chamber airtight window 63 are all opened, so that the reagent preparation chamber 32, the sample preparation chamber 42, the gene amplification chamber 52 and the product analysis chamber 62 are respectively communicated with the outside and naturally ventilate, the reagent preparation region independent air supply device 35, the sample preparation region independent air supply device 45, the gene amplification region independent air supply device 55 and the product analysis region independent air supply device 65 are all closed and are in a stop state, and the reagent preparation region independent air exhaust device 34, the sample preparation region independent air exhaust device 44, the gene amplification region independent air exhaust device 54 and the product analysis region independent air exhaust device 64 are all in an open state, not only can guarantee good independent ventilation, and can need not to set up and purify air conditioning unit, open exhaust device can, the energy can be saved and need not extravagant space and settle and purify air conditioning unit. When encountering Severe Acute Respiratory Syndrome (SARS), human infection avian influenza, Middle East Respiratory Syndrome (MERS), novel coronavirus pneumonia (COVID-19) and other epidemic situations, the device can be operated in the second state, so that the reagent preparation chamber airtight window 33, the sample preparation chamber airtight window 43, the gene amplification chamber airtight window 53 and the product analysis chamber airtight window 63 are all closed, so that the reagent preparation chamber 32, the sample preparation chamber 42, the gene amplification chamber 52 and the product analysis chamber 62 are respectively sealed and isolated, and simultaneously the reagent preparation area independent air supply device 35, the sample preparation area independent air supply device 45, the gene amplification area independent air supply device 55, the product analysis area independent air supply device 65, the reagent preparation area independent air exhaust device 34, the sample preparation area independent air exhaust device 44, the gene amplification area independent air exhaust device 54 and the product analysis area independent air exhaust device 64 are opened, so that air supply and exhaust of each area are respectively and independently performed, effectively ensures the safety performance of each region and is extremely suitable for epidemic PCR projects.

From the above, the dual-purpose PCR laboratory system provided in the embodiment of the present application effectively solves the technical problems that in the prior art, each area of a PCR laboratory cannot absolutely isolate air from each other, which easily causes health threat of medical research and inspection personnel and inaccurate detection result, and the PCR laboratory fails when encountering an epidemic situation, realizes relatively independent ventilation between each area of the PCR laboratory, does not have cross contamination, has high safety performance, is applicable both in a normal state and in an epidemic situation, does not need to temporarily build or modify the PCR laboratory when encountering an epidemic situation, effectively ensures immediate use under special situations such as an epidemic situation, can realize general use under normal situations and special situations by one-time building, and greatly saves cost and time for new building or modification.

In the present embodiment, the reagent preparing section independent air exhausting device 34 is configured to simultaneously exhaust the air in the reagent preparing compartment 32 and the air in the reagent preparing buffer compartment 31 to the outside.

In this embodiment, the independent exhaust device 34 in the reagent preparation area includes a main independent exhaust pipe 341 in the reagent preparation area, an independent exhaust fan 342 in the reagent preparation area, an efficient exhaust filter 343 in the reagent preparation area, an exhaust branch pipe 344 in the reagent preparation buffer room, and an exhaust branch pipe 345 in the reagent preparation room.

The main independent exhaust pipe 341 of the reagent preparation area is disposed in the reagent preparation room 32, and one end of the main independent exhaust pipe 341 of the reagent preparation area is communicated with the outside, which is an exhaust end.

The reagent preparing area independent exhaust fan 342 is disposed on the reagent preparing area independent exhaust main pipe 341 and configured to provide exhaust power.

The reagent preparing zone exhaust high efficiency filter 343 is provided on the reagent preparing zone independent exhaust main pipe 341 and configured to filter the exhausted air to prevent harmful substances and germs from being exhausted. The reagent preparation area exhaust high-efficiency filter 343 may be disposed on the exhaust side of the reagent preparation area independent exhaust fan 342 on the reagent preparation area independent exhaust main pipe 341.

One end of the reagent preparation buffering room exhaust branch pipe 344 is communicated with the reagent preparation area independent exhaust main pipe 341, and the other end of the reagent preparation buffering room exhaust branch pipe 344 is communicated with the reagent preparation buffering room 31.

One end of the exhaust branch pipe 345 of the reagent preparation room is communicated with the independent exhaust main pipe 341 of the reagent preparation area, and the other end of the exhaust branch pipe 345 of the reagent preparation room is communicated with the reagent preparation room 32.

Through the operation of the independent exhaust blower 342 in the reagent preparation area, the air in the reagent preparation buffer room 31 enters the main independent exhaust duct 341 in the reagent preparation area through the exhaust branch pipe 344 in the reagent preparation buffer room, the air in the reagent preparation room 32 enters the main independent exhaust duct 341 in the reagent preparation area through the exhaust branch pipe 345 in the reagent preparation room, and the independent exhaust blower 342 in the reagent preparation area exhausts the air to the high-efficiency exhaust filter 343 in the reagent preparation area, and the air is filtered by the high-efficiency exhaust filter 343 in the reagent preparation area and then exhausted to the outside. The simultaneous discharge of the air in the reagent preparing compartment 32 and the air in the reagent preparing buffer compartment 31 is achieved.

Preferably, the reagent preparing area independent exhaust device 34 further includes a reagent preparing buffer room exhaust adjusting valve 346 and a reagent preparing room exhaust adjusting valve 347.

The reagent preparing buffer room exhaust regulating valve 346 is arranged on the reagent preparing buffer room exhaust branch pipe 344. The exhaust regulating valve 347 of the reagent preparation room is arranged on the exhaust branch pipe 345 of the reagent preparation room. By adopting the arrangement, the air exhaust speed and the air exhaust amount of the reagent preparation buffer room 31 and the air exhaust speed and the air exhaust amount of the reagent preparation room 32 can be conveniently and respectively adjusted, so that the air pressure can be conveniently adjusted.

Preferably, the independent exhaust device 34 of the reagent preparation area further includes a main exhaust adjusting valve 348 of the reagent preparation area, and the main exhaust adjusting valve 348 of the reagent preparation area is disposed on the main independent exhaust pipe 341 of the reagent preparation area and is located on the air intake side of the independent exhaust fan 342 of the reagent preparation area. With the above arrangement, the total exhaust flow of the main independent exhaust pipe 341 in the reagent preparation area can be conveniently adjusted. The reagent preparation buffer room air exhaust adjusting valve 346 and the reagent preparation room air exhaust adjusting valve 347 are conveniently matched to perform integral accurate adjustment.

Preferably, the reagent preparation area independent exhaust device 34 further includes a reagent preparation area exhaust check valve 349, and the reagent preparation area exhaust check valve 349 is disposed on the reagent preparation area independent exhaust main pipe 341 and is located on the air outlet side of the reagent preparation area independent exhaust fan 342. With the above arrangement, the backflow of air into the reagent preparing compartment 32 and the reagent preparing buffer compartment 31 can be prevented.

Preferably, the end of the reagent preparation buffer compartment exhaust branch pipe 344 communicating with the reagent preparation buffer compartment 31 is located at the entrance of the reagent preparation buffer compartment 31 away from the reagent preparation buffer compartment 31 and at the entrance of the reagent preparation compartment 32.

Preferably, the end of the reagent preparation room exhaust manifold 345 that communicates with the reagent preparation room 32 is located at an entrance door of the reagent preparation room 32 that is remote from the reagent preparation room 32. By adopting the arrangement, the airflow flows from the low pollution risk area to the high pollution risk area.

Preferably, the end of the reagent preparing buffer room exhaust branch pipe 344 communicated with the reagent preparing buffer room 31 may be located at the lower part of the reagent preparing buffer room 31, and the end of the reagent preparing room exhaust branch pipe 345 communicated with the reagent preparing room 32 may be located at the lower part of the reagent preparing room 32, so that the air flow is not exhausted through the upper part of the room, and the pollution to the reagent preparing room 32 and the infection risk of personnel are reduced.

Preferably, one end of the reagent preparation buffer room exhaust branch pipe 344 communicated with the reagent preparation buffer room 31 may be communicated with the reagent preparation buffer room 31 through an aluminum alloy single-layer vertical barrier exhaust louver. One end of the exhaust branch pipe 345 of the reagent preparation room communicated with the reagent preparation room 32 can be communicated with the reagent preparation room 32 through an aluminum alloy single-layer vertical grid exhaust shutter.

In the present embodiment, the sample-preparation-area independent air-discharge device 44 is configured to discharge the air in the sample preparation compartment 42 and the air in the sample preparation buffer compartment 41 to the outside at the same time.

In this embodiment, the sample preparation area independent exhaust device 44 includes a sample preparation area independent exhaust main pipe 441, a sample preparation area independent exhaust fan 442, a sample preparation area exhaust high-efficiency filter 443, a sample preparation buffer chamber exhaust branch pipe 444, and a sample preparation chamber exhaust branch pipe 445.

The sample preparation area independent air exhaust main pipe 441 is arranged in the sample preparation room 42, and one end of the sample preparation area independent air exhaust main pipe 441 is communicated with the outside.

The sample preparation area independent exhaust fan 442 is disposed on the sample preparation area independent exhaust main pipe 441 and configured to provide exhaust power.

The sample preparation area exhaust high-efficiency filter 443 is disposed on the sample preparation area independent exhaust main pipe 441, and is configured to filter the exhausted air and prevent harmful substances and germs from being exhausted. The sample preparation area exhaust high-efficiency filter 443 may be disposed on the exhaust side of the sample preparation area independent exhaust fan 442 on the sample preparation area independent exhaust main pipe 441.

One end of the sample preparation buffer room exhaust branch pipe 444 is communicated with the sample preparation area independent exhaust main pipe 441, and the other end of the sample preparation buffer room exhaust branch pipe 444 is communicated with the sample preparation buffer room 41.

One end of the exhaust branch pipe 445 of the sample preparation room is communicated with the independent exhaust main pipe 441 of the sample preparation area, and the other end of the exhaust branch pipe 445 of the sample preparation room is communicated with the sample preparation room 42.

The sample preparation area independent exhaust fan 442 operates, air in the sample preparation buffer room is exhausted to the sample preparation area independent exhaust main pipeline 441 through the sample preparation buffer room exhaust branch pipe 444, air in the sample preparation room is exhausted to the sample preparation area independent exhaust main pipeline 441 through the sample preparation room exhaust branch pipe 445, the sample preparation area independent exhaust fan 442 exhausts the air to the sample preparation area exhaust high-efficiency filter 443, and the air is exhausted to the outside through the sample preparation area exhaust high-efficiency filter 443.

Preferably, the sample preparation area independent exhaust device 44 further comprises a sample preparation buffer exhaust adjustment valve 446 and a sample preparation compartment exhaust adjustment valve 447.

The sample preparation buffer room exhaust regulating valve 446 is arranged on the sample preparation buffer room exhaust branch pipe 444; the sample preparation room exhaust regulating valve 447 is arranged on the sample preparation room exhaust branch pipe 445.

Preferably, the sample preparation area independent exhaust device 44 further includes a sample preparation area exhaust main adjusting valve 448, and the sample preparation area exhaust main adjusting valve 448 is disposed on the sample preparation area independent exhaust main pipe 441 and is located on the air intake side of the sample preparation area independent exhaust fan 442.

Preferably, the sample preparation area independent exhaust device 44 further includes a sample preparation area exhaust check valve 449, and the sample preparation area exhaust check valve 449 is disposed on the sample preparation area independent exhaust main pipe 441 and is located on the air outlet side of the sample preparation area independent exhaust fan 442.

Preferably, the end of the sample preparation buffer compartment exhaust branch 444 communicating with the sample preparation buffer compartment 41 is located at the entrance of the sample preparation buffer compartment 41 away from the sample preparation buffer compartment 41 and at the entrance of the sample preparation compartment 42.

Preferably, the end of the sample preparation room exhaust branch 445 communicated with the sample preparation room 42 is located at the entrance door of the sample preparation room 42 away from the sample preparation room 42. By adopting the arrangement, the airflow flows from the low pollution risk area to the high pollution risk area.

In this embodiment, the gene amplification region independent air exhausting device 54 is configured to simultaneously exhaust the air in the gene amplification chamber 52 and the air in the gene amplification buffer chamber 51 to the outside.

Preferably, the gene amplification region independent exhaust device 54 includes a gene amplification region independent exhaust main pipe 541, a gene amplification region independent exhaust fan 542, a gene amplification region exhaust high-efficiency filter 543, a gene amplification buffer chamber exhaust branch pipe 544, and a gene amplification chamber exhaust branch pipe 545.

The main independent air exhaust pipe 541 of the gene amplification area is arranged in the gene amplification room 52, and one end of the main independent air exhaust pipe 541 of the gene amplification area is communicated with the outside.

The gene amplification region independent exhaust fan 542 is disposed on the gene amplification region independent exhaust main pipe 541, and is configured to provide exhaust power.

The gene amplification region exhaust high-efficiency filter 543 is disposed on the gene amplification region independent exhaust main pipe 541, and is configured to filter the exhaust air.

One end of the exhaust branch pipe 544 of the gene amplification buffer room is communicated with the main independent exhaust pipe 541 of the gene amplification region, and the other end of the exhaust branch pipe 544 of the gene amplification buffer room is communicated with the gene amplification buffer room 51.

One end of the exhaust branch pipe 545 between the gene amplifications is communicated with the main independent exhaust pipe 541 of the gene amplification region, and the other end of the exhaust branch pipe 545 between the gene amplifications is communicated with the gene amplification chamber 52.

The independent exhaust fan 542 in the gene amplification region works to provide exhaust power, so that the air in the gene amplification buffer chamber enters the independent exhaust main pipeline 541 in the gene amplification region through the gene amplification buffer chamber, the air in the gene amplification chamber enters the independent exhaust main pipeline 541 in the gene amplification region through the exhaust branch pipe 545 in the gene amplification chamber, and is filtered by the efficient exhaust filter 543 in the gene amplification region and then is exhausted outdoors.

Preferably, the gene amplification region independent air exhaust device 54 further comprises a gene amplification buffer room air exhaust regulating valve 546 and a gene amplification room air exhaust regulating valve 547.

The gene amplification buffer room exhaust regulating valve 546 is arranged on the gene amplification buffer room exhaust branch pipe 544; the exhaust regulating valve 547 is installed on the exhaust branch pipe 545 in the gene amplification chamber.

Preferably, the gene amplification region independent exhaust device 54 further comprises a gene amplification region main exhaust air regulating valve 548, wherein the gene amplification region main exhaust air regulating valve 548 is arranged on the gene amplification region independent exhaust main pipe 541 and is positioned on the air inlet side of the gene amplification region independent exhaust fan 542.

Preferably, the gene amplification region independent exhaust device 54 further comprises a gene amplification region exhaust check valve 549, and the gene amplification region exhaust check valve 549 is arranged on the gene amplification region independent exhaust main pipe 541 and is located on the air outlet side of the gene amplification region independent exhaust fan 542.

Preferably, the end of the exhaust branch 544 communicating with the gene amplification buffer room 51 is located at the entrance of the gene amplification buffer room 51 far from the gene amplification buffer room 51 and at the entrance far from the gene amplification buffer room 52.

Preferably, the end of the exhaust branch 545 of the gene amplification chamber which communicates with the gene amplification chamber 52 is located at the entrance of the gene amplification chamber 52 which is far from the gene amplification chamber 52.

In the present embodiment, the product analysis section independent air discharge device 64 is configured to discharge the air in the product analysis compartment 62 and the air in the product analysis buffer compartment 61 to the outside at the same time.

In this embodiment, the product analysis area independent exhaust device 64 includes a product analysis area independent exhaust main pipe 641, a product analysis area independent exhaust fan 642, a product analysis area exhaust high-efficiency filter 643, a product analysis buffer compartment exhaust branch pipe 644, and a product analysis compartment exhaust branch pipe 645.

The main independent exhaust pipe 641 in the product analysis area is disposed in the product analysis room 62, and one end of the main independent exhaust pipe 641 in the product analysis area is communicated with the outside.

The product analysis area independent exhaust fan 642 is disposed on the product analysis area independent exhaust main pipe 641 and configured to provide exhaust power.

A product analysis zone vent high efficiency filter 643 is disposed on the product analysis zone independent vent main duct 641 and configured to filter the exhausted air.

One end of the exhaust branch pipe 644 of the product analysis buffer room is communicated with the independent exhaust main pipe 641 of the product analysis area, and the other end of the exhaust branch pipe 644 of the product analysis buffer room is communicated with the product analysis buffer room 61.

One end of the exhaust branch pipe 645 of the product analysis room is communicated with the independent exhaust main pipe 641 of the product analysis area, and the other end of the exhaust branch pipe 645 of the product analysis room is communicated with the product analysis room 62.

The independent exhaust fan 642 of the product analysis area works to provide exhaust power, so that the air in the product analysis buffer room is exhausted to the independent exhaust main pipe 641 of the product analysis area through the product analysis room, the air in the product analysis room is exhausted to the independent exhaust main pipe 641 of the product analysis area through the exhaust branch pipe 645 of the product analysis room, and is exhausted to the outside after being filtered by the exhaust high-efficiency filter 643 of the product analysis area.

Preferably, the product analysis zone independent exhaust device 64 further includes a product analysis buffer exhaust regulating valve 646 and a product analysis room exhaust regulating valve 647.

The product analysis buffer room exhaust regulating valve 646 is arranged on the product analysis buffer room exhaust branch pipe 644; the product analysis room exhaust regulating valve 647 is arranged on the product analysis room exhaust branch pipe 645.

Preferably, the product analysis area independent exhaust device 64 further comprises a product analysis area main exhaust air regulating valve 648, which is disposed on the product analysis area independent exhaust main pipe 641 and is located on the air intake side of the product analysis area independent exhaust fan 642.

Preferably, the product analysis area independent exhaust device 64 further includes a product analysis area exhaust check valve 649 disposed on the product analysis area independent exhaust main pipe 641 and located on the air outlet side of the product analysis area independent exhaust fan 642.

Preferably, product analysis buffer exhaust branch 644 communicates with product analysis buffer compartment 61 at an end of product analysis buffer compartment 61 remote from the entrance of product analysis buffer compartment 61 and remote from the entrance of product analysis compartment 62.

Preferably, the end of product analysis compartment exhaust manifold 645 in communication with product analysis compartment 62 is located at an entrance to product analysis compartment 62 remote from product analysis compartment 62.

In this embodiment, the independent air supply device 35 for the reagent preparation area, the independent air supply device 45 for the sample preparation area, the independent air supply device 55 for the gene amplification area, and the independent air supply device 65 for the product analysis area are independent of each other and are not communicated with each other, so that independent air intake and independent air exhaust are realized among the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52, and the product analysis room 62, which are not interfered with each other and do not affect each other, thereby preventing cross infection and effectively ensuring the safety performance of single rooms.

In this embodiment, the reagent preparing section independent air blowing device 35 is configured to simultaneously blow air into the reagent preparing compartment 32 and the reagent preparing buffer compartment 31.

Preferably, the reagent preparing zone independent air blowing device 35 includes a reagent preparing zone independent air blowing main pipe 351, a reagent preparing buffer room air blowing branch pipe 352, a reagent preparing zone independent air blower 353, and a reagent preparing zone air blowing filter 354.

The main independent air supply pipe 351 of the reagent preparation area penetrates through the reagent preparation buffer room 31 and the reagent preparation room 32, one end of the main independent air supply pipe 351 of the reagent preparation area is communicated with the outside, and the other end of the main independent air supply pipe is communicated with the reagent preparation room 32.

One end of the reagent preparation buffer compartment air supply branch pipe 352 is communicated with the reagent preparation area independent air supply main pipe 351, and the other end thereof is communicated with the reagent preparation buffer compartment 31.

The reagent preparing area independent blower 353 is provided on the reagent preparing area independent blowing main pipe 351 and configured to supply blowing power.

The reagent preparation area air supply filter 354 is provided on the reagent preparation area independent air supply main pipe 351 and is configured to filter air supplied to the reagent preparation room 32 and the reagent preparation buffer room 31. Reagent preparation zone feed filter 354 may be a primary filter or a high efficiency filter.

The reagent preparation area independent air feeder 353 provides power to lead air to the reagent preparation area air feeding filter 354, the air is filtered by the reagent preparation area air feeding filter 354 and then enters the reagent preparation area independent air feeding main pipeline 351, the air is discharged into the reagent preparation buffer room through the reagent preparation buffer room air feeding branch pipe 352, and meanwhile, one end of the reagent preparation area independent air feeding main pipeline 351, which is positioned in the reagent preparation room 32, feeds air into the reagent preparation room 32. Thereby realizing the introduction of fresh air.

Preferably, the reagent preparation area independent air supply device 35 further includes a reagent preparation buffer room air supply adjusting valve 355 and a reagent preparation room air supply adjusting valve 356.

The reagent preparation buffer room air supply adjusting valve 355 is disposed on the reagent preparation buffer room air supply branch pipe 352. The reagent preparation room air supply adjusting valve 356 is disposed on the reagent preparation area independent air supply main pipe 351 and is located in the reagent preparation room 32. With the arrangement, the air quantity sent into the reagent preparation buffering room can be adjusted through the reagent preparation buffering room air supply adjusting valve 355, and the air quantity sent into the reagent preparation room can be adjusted through the reagent preparation room air supply adjusting valve 356, so that the reagent preparation buffering room air exhaust adjusting valve 346 and the reagent preparation room air exhaust adjusting valve 347 are conveniently matched to adjust the air exhaust quantity and the air supply quantity of the reagent preparation buffering room and the air exhaust quantity and the air supply quantity of the reagent preparation room, and the air pressure of the reagent preparation room and the air pressure of the reagent preparation buffering room are adjusted. Alternatively, the air exhaust amount of the reagent preparation buffer room is 180CMH, the air supply amount is 120CMH, the air exhaust amount of the reagent preparation buffer room is 300CMH, the air supply amount is 340CMH, and the infiltration air amount is 40CMH, so that the air pressure of the reagent preparation buffer room is 0PA and the air pressure of the reagent preparation room is +10PA, through the matching adjustment of the reagent preparation buffer room air supply adjusting valve 355, the reagent preparation buffer room air supply adjusting valve 356, the reagent preparation buffer room air exhaust adjusting valve 346, and the reagent preparation room air exhaust adjusting valve 347.

Preferably, reagent preparation zone independent air supply arrangement 35 further includes a reagent preparation zone air supply first main regulator valve 357 and a reagent preparation zone air supply second main regulator valve 358.

The reagent preparation area air supply first main adjustment valve 357 is disposed on the reagent preparation area independent air supply main pipe 351 and located on the air intake side of the reagent preparation area independent air supply blower 353.

The reagent preparation area air supply second main adjustment valve 358 is disposed on the reagent preparation area independent air supply main pipe 351 and is located on the air outlet side of the reagent preparation area independent air supply unit 353.

With the above arrangement, the air supply amount can be conveniently and integrally regulated and controlled by arranging the first main regulating valve 357 for air supply in the reagent preparation area and the second main regulating valve 358 for air supply in the reagent preparation area.

Preferably, one end of the main independent exhaust pipe 341 of the reagent preparation area, which is communicated with the outside, penetrates through the reagent preparation room 32 along one side far away from the reagent preparation buffer room 31; one end of the main independent air supply pipe 351 of the reagent preparation area, which is communicated with the outside, penetrates through the reagent preparation room 32, the reagent preparation buffer room 31 and the special PCR walkway 20 along one side close to the reagent preparation buffer room 31. Set up more than adopting for reagent preparation district independently airs exhaust main pipe 341's air exit and reagent preparation district independently air supply main pipe 351's air intake separates to both ends respectively, prevents that reagent preparation district independently airs exhaust main pipe 341's exhaust air from getting into reagent preparation district independently air supply main pipe 351 again.

In the present embodiment, the sample preparation section independent air blowing device 45 is configured to simultaneously blow air into the sample preparation compartment 42 and the sample preparation buffer compartment 41.

Preferably, the specimen preparation area independent air blowing device 45 includes a main specimen preparation area independent air blowing duct 451, a specimen preparation buffer compartment air blowing branch duct 452, a specimen preparation area independent air blowing machine 453, and a specimen preparation area air blowing filter 454.

The sample preparation area independent air supply main pipeline 451 penetrates through the sample preparation buffer room 41 and the sample preparation room 42; one end of the main independent air supply duct 451 of the sample preparation area communicates with the outside, and the other end communicates with the sample preparation room 42.

One end of the sample preparation buffer compartment air supply branch pipe 452 is communicated with the sample preparation area independent air supply main pipe 451, and the other end thereof is communicated with the sample preparation buffer compartment 41.

The sample preparation area independent blower 453 is provided on the sample preparation area independent blowing main pipe 451 and configured to supply blowing power.

The sample preparation area air supply filter 454 is provided on the sample preparation area independent air supply main pipe 451, and is configured to filter air supplied to the sample preparation compartment 42 and the sample preparation buffer compartment 41. The sample preparation area supply air filter 454 may be a primary filter or a high efficiency filter.

The sample preparation area independent air supply device 453 supplies power to guide air to the sample preparation area air supply filter 454, the air is filtered by the sample preparation area air supply filter 454, enters the sample preparation area independent air supply main pipeline 451, and is discharged to the sample preparation buffer room through the sample preparation buffer room air supply branch pipe 452, and meanwhile, one end of the sample preparation area independent air supply main pipeline 451, which is positioned in the sample preparation room 42, supplies air to the sample preparation room 42. Thereby realizing the introduction of fresh air.

Preferably, the sample preparation area independent air supply device 45 further includes a sample preparation buffer compartment air supply adjusting valve 455 and a sample preparation compartment air supply adjusting valve 456.

The sample preparation buffer compartment air supply adjusting valve 455 is provided on the sample preparation buffer compartment air supply branch pipe 452. The sample preparation room air supply adjusting valve 456 is provided on the sample preparation area independent air supply main pipe 451, and is located in the sample preparation room 42. With the arrangement, the air volume sent into the sample preparation buffer room can be adjusted through the sample preparation buffer room air supply adjusting valve 455, and the air volume sent into the sample preparation room can be adjusted through the sample preparation room air supply adjusting valve 456, so that the sample preparation buffer room air exhaust adjusting valve 446 and the sample preparation room air exhaust adjusting valve 447 can be conveniently matched to adjust the air exhaust volume and the air supply volume of the sample preparation buffer room and the air exhaust volume and the air supply volume of the sample preparation room, and the air pressure of the sample preparation buffer room and the air pressure of the sample preparation room can be adjusted. Alternatively, the air exhaust amount of the sample preparation buffer room is 110CMH and the air supply amount of the sample preparation buffer room is 120CMH, the air exhaust amount of the sample preparation buffer room is 1530CMH and the air supply amount of the sample preparation room is 1500CMH when one biosafety cabinet is opened, the air exhaust amount of the sample preparation room is 3030CMH and the air supply amount of the sample preparation room is 3000CMH when two biosafety cabinets are opened, so that the air pressure of the sample preparation buffer room is 0PA and the air pressure of the sample preparation room is-10 PA by matching and adjusting the sample preparation buffer room air supply adjusting valve 455, the sample preparation buffer room air exhaust adjusting valve 446 and the sample preparation room air supply adjusting valve 447.

Preferably, sample preparation zone independent air supply arrangement 45 also includes a sample preparation zone air supply first main adjustment valve 457 and a sample preparation zone air supply second main adjustment valve 458.

The sample preparation area independent air supply main pipe 451 is provided with a first main adjustment valve 457, which is located on the air inlet side of the sample preparation area independent air supply machine 453.

The second main sample preparation area air supply adjustment valve 458 is disposed on the main sample preparation area independent air supply pipe 451 and is located on the air outlet side of the sample preparation area independent air supply blower 453.

Preferably, one end of the main independent exhaust duct 441 in the sample preparation area, which is communicated with the outside, penetrates through the sample preparation room 42 along one side far away from the sample preparation buffer room 41; one end of the main independent air supply pipe 451 of the sample preparation area, which is communicated with the outside, penetrates through the sample preparation room 42, the sample preparation buffer room 41 and the PCR lane 20 along one side close to the sample preparation buffer room 41. With the arrangement, the air outlet of the main pipe 441 for independently exhausting air in the sample preparation area and the air inlet of the main pipe 451 for independently supplying air in the sample preparation area are separated from each other towards two ends, so that the air exhausted from the main pipe 441 for independently exhausting air in the sample preparation area is prevented from reentering the main pipe 451 for independently supplying air in the sample preparation area.

In this embodiment, the gene amplification region independent air blowing device 55 is configured to blow air into the gene amplification chamber 52 and the gene amplification buffer chamber 51 at the same time.

Preferably, the gene amplification region independent air blowing means 55 comprises a main gene amplification region independent air blowing pipe 551, a gene amplification buffer compartment air blowing branch pipe 552, a gene amplification region independent air blowing fan 553, and a gene amplification region air blowing filter 554.

The main independent air supply pipeline 551 of the gene amplification area penetrates through the gene amplification buffer room 51 and the gene amplification room 52; one end of the main independent air supply pipeline 551 of the gene amplification region is communicated with the outside, and the other end is communicated with the gene amplification chamber 52.

One end of the gene amplification buffer room air supply branch pipe 552 is communicated with the gene amplification region independent air supply main pipe 551, and the other end thereof is communicated with the gene amplification buffer room 51.

The gene amplification region independent air blower 553 is provided on the gene amplification region independent air blowing main pipe 551 and is configured to supply air blowing power.

The gene amplification region air supply filter 554 is provided on the main gene amplification region independent air supply line 551, and is configured to filter air supplied to the gene amplification chamber 52 and the gene amplification buffer chamber 51. The gene amplification region air supply filter 554 may be a primary filter or a high efficiency filter.

The air is introduced to the gene amplification region air supply filter 554 by the independent air supply blower 553 of the gene amplification region, filtered by the gene amplification region air supply filter 554, introduced into the main independent air supply pipe 551 of the gene amplification region, and supplied into the gene amplification buffer compartment through the main independent air supply pipe 552 of the gene amplification buffer compartment, while the end of the main independent air supply pipe 551 of the gene amplification region located in the gene amplification compartment supplies air into the gene amplification compartment.

Preferably, the gene amplification region independent air supply device 55 further includes a gene amplification buffer compartment air supply regulation valve 555 and a gene amplification compartment air supply regulation valve 556.

The gene amplification buffer room air supply regulating valve 555 is provided on the gene amplification buffer room air supply branch pipe 552. The inter-gene amplification air supply regulating valve 556 is provided on the main gene amplification region independent air supply pipe 551 and is located in the gene amplification chamber 52. With the arrangement, the air quantity entering the gene amplification buffer room can be adjusted through the gene amplification buffer room air supply adjusting valve 555, and the air quantity entering the gene amplification room can be adjusted through the gene amplification room air supply adjusting valve 556, so that the air quantity and the air quantity of the gene amplification buffer room and the air quantity of the gene amplification room can be adjusted conveniently by matching with the gene amplification buffer room air exhaust adjusting valve and the gene amplification room air exhaust adjusting valve, and the air pressure of the gene amplification buffer room and the air pressure of the gene amplification room can be adjusted. Alternatively, the air discharge amount of the gene amplification buffer room is set to 110CMH, the air supply amount is set to 120CMH, the permeation air amount is set to 30CMH, the air discharge amount of the gene amplification buffer room is set to 370CMH, the air supply amount is set to 340CMH, the air pressure of the gene amplification buffer room is set to 0PA, and the air pressure of the gene amplification room is set to-15 PA by the cooperative adjustment of the gene amplification buffer room air supply adjusting valve 555, the gene amplification buffer room air supply adjusting valve 556, the gene amplification buffer room air discharge adjusting valve, and the gene amplification room air discharge adjusting valve.

Preferably, the gene amplification region independent air blowing means 55 further comprises a gene amplification region air blowing first main adjustment valve 557 and a gene amplification region air blowing second main adjustment valve 558.

The first main control valve 557 for gene amplification region blowing is disposed on the main duct 551 for gene amplification region independent blowing, and is located on the air intake side of the gene amplification region independent blowing machine 553.

The second main regulation valve 558 is disposed on the main independent blowing pipe 551 for gene amplification region and located on the air outlet side of the independent blowing fan 553 for gene amplification region.

Preferably, one end of the main independent exhaust duct 541 in the gene amplification area, which is communicated with the outside, penetrates through the gene amplification chamber 52 along one side far away from the gene amplification buffer chamber 51; one end of the main independent air supply pipeline 551 of the gene amplification area, which is communicated with the outside, penetrates through the gene amplification room 52, the gene amplification buffer room 51 and the special PCR walkway 20 along one side close to the gene amplification buffer room 51. With the arrangement, the air outlet of the main independent air exhaust pipe 541 in the gene amplification area and the air inlet of the main independent air supply pipe 551 in the gene amplification area are separated towards two ends respectively, so that the air exhausted from the main independent air exhaust pipe 541 in the gene amplification area is prevented from reentering the main independent air supply pipe 551 in the gene amplification area.

In the present embodiment, the product analysis area independent air blowing device 65 is configured to simultaneously blow air into the product analysis compartment 62 and the product analysis buffer compartment 61.

Preferably, the product analysis zone independent air blowing device 65 includes a product analysis zone independent air blowing main pipe 651, a product analysis buffer compartment air blowing branch pipe 652, a product analysis zone independent air blowing machine 653, and a product analysis zone air blowing filter 654.

The main independent air supply pipe 651 in the product analysis area penetrates through the product analysis buffer room 61 and the product analysis room 62; one end of the main independent air supply pipe 651 of the product analysis region is communicated with the outside, and the other end thereof is communicated with the product analysis room 62.

One end of the product analysis buffer room air supply branch pipe 652 is communicated with the product analysis area independent air supply main pipe 651, and the other end thereof is communicated with the product analysis buffer room 61.

The product analysis area independent blower 653 is provided on the product analysis area independent blower main pipe 651, and is configured to supply blower power.

The product analysis zone air supply filter 654 is provided on the product analysis zone independent air supply main pipe 651, and is configured to filter the air supplied to the product analysis compartment 62 and the product analysis buffer compartment 61. The product analysis area independent blower 653 may be a primary filter or a high efficiency filter.

The independent blower 653 in the product analysis area supplies air to the product analysis area air supply filter 654, and the air is filtered by the product analysis area air supply filter 654, enters the main independent air supply pipe 651 in the product analysis area, and is sent to the product analysis buffer room through the branch air supply pipe 652 in the product analysis buffer room, and meanwhile, the main independent air supply pipe 651 in the product analysis area supplies air to the product analysis room from one end of the product analysis room.

Preferably, product analysis zone independent air supply 65 further includes a product analysis buffer room air supply adjustment valve 655 and a product analysis room air supply adjustment valve 656.

Product analysis buffer room air supply adjustment valve 655 is disposed on product analysis buffer room air supply branch 652. And a product analysis room air supply regulating valve 656 which is arranged on the main independent air supply pipe 651 of the product analysis area and is positioned in the product analysis room 62. With the above arrangement, the air volume entering the product analysis buffer room can be adjusted by the product analysis buffer room air supply adjusting valve 655, and the air volume entering the product analysis room can be adjusted by the product analysis room air supply adjusting valve 656, so that the product analysis buffer room air exhaust adjusting valve 646 and the product analysis room air exhaust adjusting valve 647 can be conveniently matched to adjust the air exhaust volume and the air supply volume of the product analysis buffer room and the air exhaust volume and the air supply volume of the product analysis room, and the air pressure of the product analysis buffer room and the air pressure of the product analysis room can be adjusted. Alternatively, the air pressure in the product analysis buffer room is set to 0PA and the air pressure in the product analysis room is set to-20 PA by adjusting the product analysis buffer room air supply adjusting valve 655, the product analysis buffer room air supply adjusting valve 656, the product analysis buffer room air discharge adjusting valve 646, and the product analysis room air discharge adjusting valve 647 in a matching manner, so that the air discharge amount in the product analysis buffer room is set to 100CMH and the air supply amount in the product analysis buffer room is set to 120CMH, and the air discharge amount in the product analysis room is set to 380CMH and the air supply amount in the product analysis room is set to 340 cm.

Preferably, product analysis zone independent air supply means 65 further comprises a product analysis zone air supply first primary adjustment valve 657 and a product analysis zone air supply second primary adjustment valve 658.

The product analysis zone independent air supply main pipe 651 is provided with a first main adjusting valve 657 for product analysis zone air supply, which is located on the air inlet side of the product analysis zone independent air supply 653.

The second main product analysis area air supply regulating valve 658 is disposed on the main product analysis area independent air supply pipe 651 on the air outlet side of the product analysis area independent air supply fan 653.

Preferably, one end of the main independent exhaust pipe 641 of the product analysis area, which is communicated with the outside, penetrates through the product analysis room 62 along one side far away from the product analysis buffer room 61; one end of the main independent air supply pipe 651 of the product analysis region, which is communicated with the outside, penetrates through the product analysis compartment 62, the product analysis buffer compartment 61 and the PCR dedicated passage 20 along one side close to the product analysis buffer compartment 61. With the above arrangement, the air outlet of the main duct 641 for independent air exhaust in the product analysis area and the air inlet of the main duct 651 for independent air supply in the product analysis area are separated from each other at two ends, so that the air exhausted from the main duct 641 for independent air exhaust in the product analysis area is prevented from reentering the main duct 651 for independent air supply in the product analysis area.

Preferably, each of the ducts of the reagent preparation zone independent air supply device 35, each of the ducts of the sample preparation zone independent air supply device 45, each of the ducts of the gene amplification zone independent air supply device 55, and each of the ducts of the product analysis zone independent air supply device 65 may be all disposed at the top of each zone to ensure the air supply effect.

Preferably, the above-mentioned regulating valves may be electric regulating valves or manual regulating valves.

Preferably, the changing room 13 includes a first changing room 131, a shower room 132 and a second changing room 133.

The first dressing room 131 is communicated with the preparation room 12 through a first sealing door 134, the air shower room 132 is communicated with the first dressing room 131 through a second sealing door 135, the second dressing room 133 is communicated with the air shower room 132 through a third sealing door 136, the second dressing room 133 is communicated with the PCR dedicated aisle 20 through a fourth sealing door 137, and the first dressing room 131 is communicated with the PCR dedicated aisle 20 through a fifth sealing door 138.

With the arrangement, when a conventional PCR project needs to be carried out, the second sealing door and the fourth sealing door are closed, the first sealing door and the fifth sealing door can be opened and closed, and personnel can enter the first dressing room through the preparation room 12 and enter the special PCR walkway 20 through the fifth sealing door. During the epidemic situation, when needing to carry out epidemic situation PCR project, close the fifth sealing door, first sealing door, second sealing door, third sealing door and fourth sealing door all can open and close, and personnel can get into first changing room through 12 between preparing to get into by the second sealing door wind and drench the room and carry out wind and drench the edulcoration degerming, get into the second changing room through the third sealing door again, can get into special pavement 20 of PCR by the fourth sealing door at last. The system realizes the separate and separate processing of the special conditions in the ordinary condition and the epidemic situation period, realizes dual purposes, and can effectively improve the safety performance of the special conditions in the epidemic situation period.

The embodiment of the present application further provides a method for operating a dual-purpose PCR laboratory system, which is suitable for the dual-purpose PCR laboratory system of any of the above embodiments, and the method for operating a dual-purpose PCR laboratory system includes:

when a conventional PCR project needs to be carried out, the dual-purpose PCR laboratory system is operated in a first state, the airtight window 33 of the reagent preparation room, the airtight window 43 of the sample preparation room, the airtight window 53 of the gene amplification room and the airtight window 63 of the product analysis room are all opened, so that the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52 and the product analysis room 62 are respectively communicated with the outside for natural ventilation, the independent air supply device 35 of the reagent preparation area, the independent air supply device 45 of the sample preparation area, the independent air supply device 55 of the gene amplification area and the independent air supply device 65 of the product analysis area are all in a stop state, and the independent air exhaust device 34 of the reagent preparation area, the independent air exhaust device 44 of the sample preparation area, the independent air exhaust device 54 of the gene amplification area and the independent air exhaust device 64 of the product analysis area are all in a working state; meanwhile, the second sealing door and the fourth sealing door are closed, the first sealing door and the fifth sealing door can be opened and closed, and personnel can enter the first dressing room through the preparation room 12 and enter the PCR special walkway 20 through the fifth sealing door, so that the personnel can enter each area.

When an epidemic situation PCR project is required, the dual-purpose PCR laboratory system is operated in the second state, the reagent preparation room airtight window 33, the sample preparation room airtight window 43, the gene amplification room airtight window 53 and the product analysis room airtight window 63 are all closed, so that the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52 and the product analysis room 62 are respectively in a closed state, the reagent preparation area independent air supply device 35, the sample preparation area independent air supply device 45, the gene amplification area independent air supply device 55 and the product analysis area independent air supply device 65 are all in a working state, and the reagent preparation area independent air exhaust device 34, the sample preparation area independent air exhaust device 44, the gene amplification area independent air exhaust device 54 and the product analysis area independent air exhaust device 64 are all in a working state. Meanwhile, the fifth sealing door is closed, the first sealing door, the second sealing door, the third sealing door and the fourth sealing door can be opened and closed, personnel can enter the first dressing room through the preparation room 12, enter the air shower room through the second sealing door to carry out air shower impurity removal and sterilization, enter the second dressing room through the third sealing door, and finally enter the PCR special walkway 20 through the fourth sealing door, so that the personnel can enter each area.

Example 2

This example differs from example 1 in that:

as shown in fig. 8. The area of the sample preparation room is larger than that of the reagent preparation room.

The reagent preparation area independent air blowing device 35 is configured to simultaneously blow air into the reagent preparation compartment 32, the reagent preparation buffer compartment 31, and the PCR exclusive path 20.

The reagent preparation area independent air supply device 35 further comprises a PCR special-purpose air supply branch pipe 359 and a PCR special-purpose air supply regulating valve 350 arranged on the PCR special-purpose air supply branch pipe 359, one end of the PCR special-purpose air supply branch pipe is communicated with the reagent preparation area independent air supply main pipe 351, the other end of the PCR special-purpose air supply branch pipe is communicated with the PCR special-purpose air supply branch pipe, and fresh air in the reagent preparation area independent air supply main pipe 351 can enter the PCR special-purpose air supply branch pipe through the PCR special-purpose air supply branch pipe under the action of the reagent preparation area independent air supply 353, so that the fresh air is provided for the PCR special-purpose air supply branch pipe. Meanwhile, the air volume sent into the PCR special walkway can be adjusted through the PCR special walkway air supply adjusting valve.

The sample preparation section independent air discharge device 44 is configured to discharge air in the sample preparation compartment 42, air in the sample preparation buffer compartment 41, and air in the PCR exclusive passage 20 to the outside at the same time.

The sample preparation area independent exhaust device 44 further comprises a PCR dedicated aisle exhaust branch pipe 4401 and a PCR dedicated aisle exhaust adjusting valve 4402 arranged on the PCR dedicated aisle exhaust branch pipe 4401, one end of the PCR dedicated aisle exhaust branch pipe is communicated with a sample preparation area independent exhaust main pipe 441, the other end of the PCR dedicated aisle exhaust branch pipe is communicated with the PCR dedicated aisle, and under the action of the sample preparation area independent exhaust fan 442, air in the PCR dedicated aisle is exhausted into the sample preparation area independent exhaust main pipe 441 through the PCR dedicated aisle exhaust branch pipe, and is finally exhausted outdoors through the sample preparation area independent exhaust main pipe 441. The air exhaust regulating valve of the PCR special walkway can regulate the air exhaust amount of the air in the PCR special walkway. The air exhaust volume and the air supply volume of the PCR special walkway can be adjusted through the matching of the PCR special walkway air exhaust adjusting valve and the PCR special walkway air exhaust adjusting valve, so that the air pressure is adjusted, optionally, the air exhaust volume in the PCR special walkway is 260CMH, the air supply volume in the PCR special walkway is 400CMH, and the air pressure is +10 PA.

By adopting the arrangement, the air supply and the air exhaust of the PCR special walkway and the adjustment of the air exhaust amount and the air supply amount are realized, and the air supply and exhaust of other areas are not influenced.

Example 3

This example differs from example 1 in that:

as shown in fig. 9. The area of the sample preparation room is larger than that of the reagent preparation room.

The reagent preparation area independent air supply device 35 further comprises a PCR special-purpose air supply branch pipe 359 and a PCR special-purpose air supply regulating valve 350 arranged on the PCR special-purpose air supply branch pipe, one end of the PCR special-purpose air supply branch pipe is communicated with the reagent preparation area independent air supply main pipe 351, the other end of the PCR special-purpose air supply branch pipe is communicated with the PCR special-purpose air supply branch pipe, and fresh air in the reagent preparation area independent air supply main pipe 351 can enter the PCR special-purpose air supply branch pipe through the PCR special-purpose air supply branch pipe under the action of the reagent preparation area independent air supply machine 353 so as to provide fresh air for the PCR special-purpose air supply branch pipe. Meanwhile, the air volume sent into the PCR special walkway can be adjusted through the PCR special walkway air supply adjusting valve.

The sample preparation area independent air exhaust device 44 comprises a first sample preparation area independent air exhaust main pipeline 401, a second sample preparation area independent air exhaust main pipeline 402, a first sample preparation area independent air exhaust fan 403, a second sample preparation area independent air exhaust fan 404, a second sample preparation buffer room air exhaust branch pipe 405, a second PCR special aisle air exhaust branch pipe 406, a first sample preparation area air exhaust efficient filter 407 and a second sample preparation area air exhaust efficient filter 408.

The main pipeline of independently airing exhaust in the first sample preparation area and the main pipeline of independently airing exhaust in the second sample preparation area are both arranged in the sample preparation room. One end of the main independent air exhaust pipeline of the first sample preparation area penetrates through the sample preparation room and is communicated with the outside, and the other end of the main independent air exhaust pipeline of the first sample preparation area is communicated with the sample preparation room. One end of the second sample preparation area independent air exhaust main pipeline penetrates through the sample preparation room and is communicated with the outside, and the other end of the second sample preparation area independent air exhaust main pipeline is communicated with the sample preparation room.

The first sample preparation area independent exhaust fan is arranged on the first sample preparation area independent exhaust main pipeline and configured to provide exhaust power so as to exhaust air in the sample preparation room to the outside through the first sample preparation area independent exhaust main pipeline. The first sample preparation area air exhaust high-efficiency filter is arranged on the first sample preparation area independent air exhaust main pipeline and is configured to filter air to be exhausted, so that the air is exhausted after being filtered.

One end of the exhaust branch pipe of the second sample preparation buffer room is communicated with the independent exhaust main pipeline of the second sample preparation area, and the other end of the exhaust branch pipe of the second sample preparation buffer room is communicated with the sample preparation buffer room so as to exhaust air in the sample preparation buffer room. One end of the second PCR special air exhaust branch pipe is communicated with the second sample preparation area independent air exhaust main pipeline, and the other end of the second PCR special air exhaust branch pipe is communicated with the PCR special air passage to exhaust air in the PCR special air passage. The second sample preparation area air exhaust high-efficiency filter is arranged on the second sample preparation area independent air exhaust main pipeline so as to filter air to be exhausted in the second sample preparation area independent air exhaust main pipeline.

The second sample preparation area independent exhaust fan is arranged on the second sample preparation area independent exhaust main pipeline and is configured to provide exhaust power, so that air in the sample preparation buffer room is exhausted to the second sample preparation area independent exhaust main pipeline through the second sample preparation buffer room exhaust branch pipe, air in the PCR special aisle is exhausted to the second sample preparation area independent exhaust main pipeline through the second PCR special aisle exhaust branch pipe, and finally the air is exhausted outdoors after being filtered by the second sample preparation area exhaust high-efficiency filter.

By adopting the arrangement, two sets of independent exhaust pipelines are arranged in the sample preparation room with a large area, air in the sample preparation room is exhausted independently so as to prevent the air from streaming to the sample preparation buffer room and the PCR special passage, and the air in the sample preparation buffer room and the air in the sample preparation room are exhausted independently through the other pipeline, so that smooth ventilation is ensured.

The sample preparation area independent air supply device 45 includes a first sample preparation area independent air supply main pipe 4501, a second sample preparation area independent air supply main pipe 4502, a first sample preparation area independent air supply machine 4503, a second sample preparation area independent air supply machine 4504, and a second sample preparation buffer room air supply branch pipe 4505.

One end of the main independent air supply pipeline of the first sample preparation area is communicated with the outside, the other end of the main independent air supply pipeline of the first sample preparation area is communicated with the sample preparation room, and air is only supplied to the sample preparation room. The independent blower in the first sample preparation area is arranged on the main independent air supply pipeline in the first sample preparation area to provide air supply power.

One end of the main pipe for independent air supply of the second sample preparation area is communicated with the outside, the other end of the main pipe for independent air supply of the second sample preparation area is communicated with the sample preparation room, one end of the air supply branch pipe between the buffer of the second sample preparation is communicated with the main pipe for independent air supply of the second sample preparation area, and the other end of the main pipe for independent air supply of the second sample preparation area is communicated with the buffer of the sample preparation room. The second sample preparation area independent blower is arranged on the second sample preparation area independent air supply main pipeline and provides air supply power.

By adopting the arrangement, two sets of independent air supply pipelines are adopted, wherein one set independently supplies air to the sample preparation room, and the other set can respectively supply air to the sample preparation room and the sample preparation buffer room. The air quantity of the air supply and the stability of the air supply are ensured.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.

Claims

1. A dual-purpose PCR laboratory system, comprising:

an entry zone, the entry zone comprising:

a clean room;

the preparation room is communicated with the clean room through a door body;

the dressing room is communicated with the preparation room through a door body;

a reagent preparation zone comprising:

the reagent preparation buffer room is communicated with the PCR special passage through a door body;

the reagent preparation room is communicated with the reagent preparation buffer room through a door body and is configured to prepare a stored reagent, split-package the reagent and prepare a main reaction mixed solution;

the reagent preparation chamber air-tight window is arranged on one side wall of the reagent preparation chamber far away from the reagent preparation buffer chamber in an opening and closing manner;

a reagent preparation zone independent air exhaust device configured to exhaust air in the reagent preparation compartment to an outside of the reagent preparation compartment;

a reagent preparation zone independent air supply device configured to deliver air into the reagent preparation compartment;

a sample preparation zone, the sample preparation zone comprising:

the sample preparation buffer room is communicated with the PCR special passage through a door body;

the sample preparation room is communicated with the sample preparation buffer room through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into the amplification reaction tube and synthesize cDNA;

a sample preparation room airtight window which is openably and closably arranged on one side wall of the sample preparation room far away from the sample preparation buffer room;

a sample preparation zone independent exhaust device configured to exhaust air in the sample preparation compartment to an exterior of the sample preparation compartment;

a sample preparation zone independent air supply device configured to deliver air into the sample preparation compartment;

a gene amplification region, said gene amplification region comprising:

the gene amplification buffer room is communicated with the PCR special walkway through a door body;

a gene amplification chamber, wherein the gene amplification chamber is communicated with the gene amplification buffer chamber through a door body, and the gene amplification chamber is configured to amplify DNA or cDNA;

the gene amplification chamber airtight window is arranged on one side wall of the gene amplification chamber far away from the gene amplification buffer chamber in an opening and closing manner;

a gene amplification region independent air exhaust device configured to exhaust air in the gene amplification chamber to the outside of the gene amplification chamber;

a gene amplification region independent air supply device configured to supply air into the gene amplification chamber; and

a product analysis zone, the product analysis zone comprising:

a product analysis buffer room communicated with the PCR special passage through a door body;

a product analysis room, wherein the product analysis room is communicated with the product analysis buffer room through a door body, and the product analysis room is configured to measure the amplified fragments;

a product analysis chamber gas seal, the product analysis chamber gas seal being openably and closably disposed on a sidewall of the product analysis chamber remote from the product analysis buffer chamber;

a product analysis zone independent exhaust configured to exhaust air in the product analysis compartment to an exterior of the product analysis compartment;

a product analysis zone independent air supply device configured to supply air into the product analysis compartment;

2. The dual-use PCR laboratory system according to claim 1, characterized in that:

the reagent preparation area independent exhaust device is configured to simultaneously exhaust air in the reagent preparation room and air in the reagent preparation buffer room to the outside;

preferably, the reagent preparation area independent air exhaust device comprises:

the reagent preparation area independent air exhaust main pipeline is arranged in the reagent preparation room, and one end of the reagent preparation area independent air exhaust main pipeline is communicated with the outside;

the reagent preparation area independent exhaust fan is arranged on the reagent preparation area independent exhaust main pipeline and is configured to provide exhaust power;

the reagent preparation area air exhaust high-efficiency filter is arranged on the reagent preparation area independent air exhaust main pipeline and is configured to filter exhausted air;

one end of the reagent preparation buffering chamber air exhaust branch pipe is communicated with the reagent preparation area independent air exhaust main pipeline, and the other end of the reagent preparation buffering chamber air exhaust branch pipe is communicated with the reagent preparation buffering chamber;

one end of the exhaust branch pipe of the reagent preparation room is communicated with the main independent exhaust pipeline of the reagent preparation area, and the other end of the exhaust branch pipe of the reagent preparation room is communicated with the reagent preparation room;

preferably, the reagent preparation area independent air exhaust device further comprises:

the reagent preparation buffer room air exhaust regulating valve is arranged on the reagent preparation buffer room air exhaust branch pipe;

the air exhaust regulating valve of the reagent preparation room is arranged on the air exhaust branch pipe of the reagent preparation room;

the reagent preparation area air exhaust main regulating valve is arranged on the reagent preparation area independent air exhaust main pipeline and is positioned on the air inlet side of the reagent preparation area independent air exhaust fan;

the reagent preparation area air exhaust check valve is arranged on the reagent preparation area independent air exhaust main pipeline and is positioned on the air outlet side of the reagent preparation area independent exhaust fan;

preferably, one end of the exhaust branch pipe of the reagent preparation buffering room, which is communicated with the reagent preparation buffering room, is positioned at a door inlet of the reagent preparation buffering room, which is far away from the reagent preparation buffering room, and a door inlet of the reagent preparation buffering room, which is far away from the reagent preparation buffering room;

preferably, one end of the exhaust branch pipe of the reagent preparation room, which is communicated with the reagent preparation room, is positioned at the door inlet of the reagent preparation room, which is far away from the reagent preparation room.

3. The dual-use PCR laboratory system according to claim 1, characterized in that:

the sample preparation area independent exhaust device is configured to simultaneously exhaust air in the sample preparation room and air in the sample preparation buffer room to the outside;

preferably, the sample preparation area independent air exhaust device comprises:

the system comprises a sample preparation area independent air exhaust main pipeline, a sample preparation area independent air exhaust main pipeline and a sample preparation room, wherein the sample preparation area independent air exhaust main pipeline is arranged in a sample preparation room, and one end of the sample preparation area independent air exhaust main pipeline is communicated with the outside;

the sample preparation area independent exhaust fan is arranged on the sample preparation area independent exhaust main pipeline and is configured to provide exhaust power;

the sample preparation area air exhaust high-efficiency filter is arranged on the sample preparation area independent air exhaust main pipeline and is configured to filter exhausted air;

one end of the sample preparation buffer room exhaust branch pipe is communicated with the sample preparation area independent exhaust main pipeline, and the other end of the sample preparation buffer room exhaust branch pipe is communicated with the sample preparation buffer room;

one end of the sample preparation room exhaust branch pipe is communicated with the sample preparation area independent exhaust main pipeline, and the other end of the sample preparation room exhaust branch pipe is communicated with the sample preparation room;

preferably, the sample preparation zone independent air exhaust device further comprises:

the sample preparation buffer room air exhaust regulating valve is arranged on the sample preparation buffer room air exhaust branch pipe;

the sample preparation room air exhaust regulating valve is arranged on the sample preparation room air exhaust branch pipe;

the sample preparation area air exhaust main regulating valve is arranged on the sample preparation area independent air exhaust main pipeline and is positioned on the air inlet side of the sample preparation area independent air exhaust fan;

the sample preparation area air exhaust check valve is arranged on the sample preparation area independent air exhaust main pipeline and is positioned on the air outlet side of the sample preparation area independent air exhaust fan;

preferably, one end of the exhaust branch pipe of the sample preparation buffer room, which is communicated with the sample preparation buffer room, is positioned at the door entrance of the sample preparation buffer room, which is far away from the sample preparation buffer room, and the door entrance of the sample preparation room;

preferably, one end of the exhaust branch pipe of the sample preparation room, which is communicated with the sample preparation room, is positioned at the door inlet of the sample preparation room, which is far away from the sample preparation room.

4. The dual-use PCR laboratory system according to claim 1, characterized in that:

the independent exhaust device of the gene amplification area is configured to simultaneously exhaust the air in the gene amplification chamber and the air in the gene amplification buffer chamber to the outside;

preferably, the gene amplification region independent air exhausting device comprises:

the gene amplification area independent air exhaust main pipeline is arranged in the gene amplification room, and one end of the gene amplification area independent air exhaust main pipeline is communicated with the outside;

the gene amplification area independent exhaust fan is arranged on the gene amplification area independent exhaust main pipeline and is configured to provide exhaust power;

the gene amplification area air exhaust high-efficiency filter is arranged on the gene amplification area independent air exhaust main pipeline and is configured to filter exhausted air;

one end of the gene amplification buffer room exhaust branch pipe is communicated with the gene amplification area independent exhaust main pipeline, and the other end of the gene amplification buffer room exhaust branch pipe is communicated with the gene amplification buffer room;

one end of the exhaust branch pipe between gene amplifications is communicated with the main independent exhaust pipeline of the gene amplification region, and the other end of the exhaust branch pipe between gene amplifications is communicated with the gene amplifications;

preferably, the gene amplification region independent exhaust device further comprises:

the gene amplification buffer room air exhaust regulating valve is arranged on the gene amplification buffer room air exhaust branch pipe;

the exhaust regulating valve between the gene amplifications is arranged on the exhaust branch pipe between the gene amplifications;

the main air exhaust regulating valve of the gene amplification area is arranged on the main independent air exhaust pipeline of the gene amplification area and is positioned on the air inlet side of the independent air exhaust fan of the gene amplification area;

the gene amplification area air exhaust check valve is arranged on the gene amplification area independent air exhaust main pipeline and is positioned on the air outlet side of the gene amplification area independent air exhaust fan;

preferably, one end of the exhaust branch pipe of the gene amplification buffer room communicated with the gene amplification buffer room is positioned at the door inlet of the gene amplification buffer room far away from the gene amplification buffer room and the door inlet of the gene amplification room far away from the gene amplification buffer room;

preferably, one end of the exhaust branch pipe of the gene amplification room communicated with the gene amplification room is positioned at the door inlet of the gene amplification room far away from the gene amplification room.

5. The dual-use PCR laboratory system according to claim 1, characterized in that:

the product analysis area independent exhaust device is configured to simultaneously exhaust air in the product analysis room and air in the product analysis buffer room to the outside;

preferably, the product analysis zone independent exhaust device comprises:

the product analysis area independent air exhaust main pipeline is arranged in the product analysis room, and one end of the product analysis area independent air exhaust main pipeline is communicated with the outside;

the product analysis area independent exhaust fan is arranged on the product analysis area independent exhaust main pipeline and is configured to provide exhaust power;

the product analysis area air exhaust high-efficiency filter is arranged on the product analysis area independent air exhaust main pipeline and is configured to filter exhausted air;

one end of the product analysis buffer room exhaust branch pipe is communicated with the product analysis area independent exhaust main pipeline, and the other end of the product analysis buffer room exhaust branch pipe is communicated with the product analysis buffer room;

one end of the exhaust branch pipe of the product analysis room is communicated with the independent exhaust main pipeline of the product analysis area, and the other end of the exhaust branch pipe of the product analysis room is communicated with the product analysis room;

preferably, the product analysis area independent exhaust device further comprises:

the product analysis buffer room air exhaust regulating valve is arranged on the product analysis buffer room air exhaust branch pipe;

the product analysis room exhaust regulating valve is arranged on the product analysis room exhaust branch pipe;

the product analysis area main exhaust adjusting valve is arranged on the product analysis area main independent exhaust pipeline and is positioned on the air inlet side of the product analysis area independent exhaust fan;

the product analysis area air exhaust check valve is arranged on the product analysis area independent air exhaust main pipeline and is positioned on the air outlet side of the product analysis area independent exhaust fan;

preferably, one end of the exhaust branch pipe of the product analysis buffer room, which is communicated with the product analysis buffer room, is positioned at the door inlet of the product analysis buffer room, which is far away from the product analysis buffer room, and the door inlet of the product analysis buffer room, which is far away from the product analysis buffer room;

preferably, one end of the exhaust branch pipe of the product analysis room, which is communicated with the product analysis room, is positioned at the door inlet of the product analysis room, which is far away from the product analysis room.

6. The dual-use PCR laboratory system according to claim 2, characterized in that:

the reagent preparation area independent air supply device is configured to simultaneously supply air to the reagent preparation room and the reagent preparation buffer room;

preferably, the reagent preparation area independent air supply device comprises:

the reagent preparation area independent air supply main pipeline penetrates through the reagent preparation buffer room and the reagent preparation room; one end of the main independent air supply pipeline of the reagent preparation area is communicated with the outside, and the other end of the main independent air supply pipeline of the reagent preparation area is communicated with the reagent preparation room;

one end of the reagent preparation buffering room air supply branch pipe is communicated with the reagent preparation area independent air supply main pipeline, and the other end of the reagent preparation buffering room air supply branch pipe is communicated with the reagent preparation buffering room;

the reagent preparation area independent air feeder is arranged on the reagent preparation area independent air feeding main pipeline and is configured to provide air feeding power;

the reagent preparation area air supply filter is arranged on the reagent preparation area independent air supply main pipeline and is configured to filter air sent into the reagent preparation room and the reagent preparation buffer room;

preferably, the reagent preparation area independent air supply device further comprises:

the reagent preparation buffer room air supply regulating valve is arranged on the reagent preparation buffer room air supply branch pipe;

the reagent preparation room air supply regulating valve is arranged on the reagent preparation area independent air supply main pipeline and is positioned in the reagent preparation room;

the reagent preparation area air supply first main regulating valve is arranged on the reagent preparation area independent air supply main pipeline and is positioned on the air inlet side of the reagent preparation area independent air supply machine;

the reagent preparation area air supply second main regulating valve is arranged on the reagent preparation area independent air supply main pipeline and is positioned on the air outlet side of the reagent preparation area independent air supply machine;

preferably, one end of the main independent exhaust pipeline of the reagent preparation area, which is communicated with the outside, penetrates through the reagent preparation room along one side far away from the reagent preparation buffer room; one end of the main independent air supply pipeline of the reagent preparation area, which is communicated with the outside, penetrates through the reagent preparation room, the reagent preparation buffer room and the PCR special walkway along one side close to the reagent preparation buffer room.

7. The dual-use PCR laboratory system according to claim 3, characterized in that:

the sample preparation area independent air supply device is configured to simultaneously supply air to the sample preparation room and the sample preparation buffer room;

preferably, the sample preparation area independent air supply device comprises:

the sample preparation area independent air supply main pipeline penetrates through the sample preparation buffer room and the sample preparation room; one end of the main independent air supply pipeline of the sample preparation area is communicated with the outside, and the other end of the main independent air supply pipeline of the sample preparation area is communicated with the sample preparation room;

one end of the sample preparation buffering room air supply branch pipe is communicated with the sample preparation area independent air supply main pipeline, and the other end of the sample preparation buffering room air supply branch pipe is communicated with the sample preparation buffering room;

the sample preparation area independent blower is arranged on the sample preparation area independent air supply main pipeline and is configured to provide air supply power;

the sample preparation area air supply filter is arranged on the sample preparation area independent air supply main pipeline and is configured to filter air sent into the sample preparation room and the sample preparation buffer room;

preferably, the sample preparation area independent air supply device further comprises:

the sample preparation buffer room air supply regulating valve is arranged on the sample preparation buffer room air supply branch pipe;

the sample preparation room air supply regulating valve is arranged on the sample preparation area independent air supply main pipeline and is positioned in the sample preparation room;

the sample preparation area air supply first main adjusting valve is arranged on the sample preparation area independent air supply main pipeline and is positioned on the air inlet side of the sample preparation area independent air supply machine;

the sample preparation area air supply second main adjusting valve is arranged on the sample preparation area independent air supply main pipeline and is positioned on the air outlet side of the sample preparation area independent air supply machine;

preferably, one end of the main independent exhaust duct of the sample preparation area, which is communicated with the outside, penetrates through the sample preparation room along one side far away from the sample preparation buffer room; one end of the sample preparation area, which is communicated with the outside, of the main independent air supply pipeline penetrates through the sample preparation room, the sample preparation buffer room and the PCR special channel along one side close to the sample preparation buffer room.

8. The dual-use PCR laboratory system according to claim 4, characterized in that:

the gene amplification region independent air supply device is configured to simultaneously supply air to the gene amplification chamber and the gene amplification buffer chamber;

preferably, the gene amplification region independent air supply device comprises:

the main independent air supply pipeline of the gene amplification area penetrates through the gene amplification buffer room and the gene amplification room; one end of the main independent air supply pipeline of the gene amplification area is communicated with the outside, and the other end of the main independent air supply pipeline of the gene amplification area is communicated with the gene amplification room;

one end of the gene amplification buffer air supply branch pipe is communicated with the gene amplification area independent air supply main pipeline, and the other end of the gene amplification buffer air supply branch pipe is communicated with the gene amplification buffer room;

the gene amplification area independent blower is arranged on the gene amplification area independent air supply main pipeline and is configured to provide air supply power;

a gene amplification region air supply filter which is arranged on the main independent air supply pipeline of the gene amplification region and is configured to filter air sent into the gene amplification chamber and the gene amplification buffer chamber;

preferably, the gene amplification region independent air supply device further comprises:

the gene amplification buffer room air supply regulating valve is arranged on the gene amplification buffer room air supply branch pipe;

the gene amplification chamber air supply regulating valve is arranged on the main independent air supply pipeline of the gene amplification area and is positioned in the gene amplification chamber;

the first main air supply regulating valve of the gene amplification area is arranged on the main independent air supply pipeline of the gene amplification area and is positioned on the air inlet side of the independent air supply machine of the gene amplification area;

the second main air supply regulating valve of the gene amplification area is arranged on the main independent air supply pipeline of the gene amplification area and is positioned on the air outlet side of the independent air supply machine of the gene amplification area;

preferably, one end of the independent air exhaust main pipeline of the gene amplification area, which is communicated with the outside, penetrates through the gene amplification chamber along one side far away from the gene amplification buffer chamber; one end of the main independent air supply pipeline of the gene amplification area, which is communicated with the outside, penetrates through the gene amplification chamber, the gene amplification buffer chamber and the PCR special walkway along one side close to the gene amplification buffer chamber.

9. The dual-use PCR laboratory system according to claim 5, characterized in that:

the product analysis zone independent air supply device is configured to simultaneously supply air to the product analysis room and the product analysis buffer room;

preferably, the product analysis zone independent air supply device comprises:

the product analysis area independent air supply main pipeline penetrates through the product analysis buffer room and the product analysis room; one end of the main independent air supply pipeline of the product analysis area is communicated with the outside, and the other end of the main independent air supply pipeline of the product analysis area is communicated with the product analysis room;

one end of the product analysis buffer room air supply branch pipe is communicated with the product analysis area independent air supply main pipeline, and the other end of the product analysis buffer room air supply branch pipe is communicated with the product analysis buffer room;

the product analysis area independent blower is arranged on the product analysis area independent air supply main pipeline and is configured to provide air supply power;

a product analysis area air supply filter arranged on the product analysis area independent air supply main pipeline and configured to filter air sent into the product analysis room and the product analysis buffer room;

preferably, the product analysis zone independent air supply device further comprises:

the product analysis buffer room air supply regulating valve is arranged on the product analysis buffer room air supply branch pipe;

the product analysis room air supply regulating valve is arranged on the main independent air supply pipeline of the product analysis area and is positioned in the product analysis room;

the product analysis area air supply first main adjusting valve is arranged on the product analysis area independent air supply main pipeline and is positioned on the air inlet side of the product analysis area independent air supply machine;

the product analysis area air supply second main adjusting valve is arranged on the product analysis area independent air supply main pipeline and is positioned on the air outlet side of the product analysis area independent air supply machine;

preferably, one end of the main independent exhaust pipe of the product analysis area, which is communicated with the outside, penetrates through the product analysis room along one side far away from the product analysis buffer room; one end of the main independent air supply pipeline of the product analysis area, which is communicated with the outside, penetrates through the product analysis room, the product analysis buffer room and the PCR special walkway along one side close to the product analysis buffer room.

10. A method of operating a dual-purpose PCR laboratory system, suitable for use in the dual-purpose PCR laboratory system according to any one of claims 1 to 9, the method comprising: