CN212743517U - PCR laboratory with dual-purpose airtight window - Google Patents
PCR laboratory with dual-purpose airtight window Download PDFInfo
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- CN212743517U CN212743517U CN202021435957.6U CN202021435957U CN212743517U CN 212743517 U CN212743517 U CN 212743517U CN 202021435957 U CN202021435957 U CN 202021435957U CN 212743517 U CN212743517 U CN 212743517U
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Abstract
The utility model discloses a PCR laboratory with dual-purpose type airtight window belongs to PCR laboratory field, the PCR laboratory with dual-purpose type airtight window is including getting into district, the special pavement of PCR, reagent preparation district, sample preparation district, gene amplification district, product analysis district, reagent preparation district airtight window, reagent preparation district screen window, sample preparation district airtight window, sample preparation district screen window, gene amplification district airtight window, product analysis district screen window. The utility model discloses a PCR laboratory with dual-purpose type airtight window has realized that each district of PCR laboratory can with external natural draft when carrying out the ordinary PCR project that need not to maintain the negative pressure, has greatly reduced energy resource consumption.
Description
Technical Field
The utility model relates to a PCR laboratory field especially relates to a PCR laboratory with airtight window of dual-purpose type.
Background
The PCR laboratory is also called a gene amplification laboratory, and the PCR laboratory generally comprises a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area. PCR is a short term for Polymerase Chain Reaction (Polymerase Chain Reaction), a molecular biology technique used to amplify specific DNA fragments and can be used as a special DNA copy in vitro. The virus content in the body of a patient can be rapidly mastered by a DNA gene tracking system, and the accuracy of the system reaches the nanometer level. For example, the number of hepatitis B viruses in a patient body, whether the hepatitis B viruses are duplicated or not, whether the hepatitis B viruses are infected or not, how strong the infection exists, whether the hepatitis B viruses need to be taken or not, whether the liver functions are abnormally changed or not can be accurately detected, which antiviral drugs are most suitable for the patient to use can be judged in time, and the curative effect of the drugs can be judged, so that a reliable inspection basis is provided for clinical treatment.
In the process of implementing the embodiments of the present invention, the inventor finds that at least the following defects exist in the background art:
because each area of the PCR laboratory needs to maintain a negative pressure state when a part of PCR projects are carried out, each area is in a closed state, fresh air is sent to each area by a conventional purification unit generally, even if a common conventional PCR project which does not need to maintain the negative pressure state is carried out, the air is still sent by the conventional purification unit, the energy consumption is high, and people are easily in a closed state for a long time to feel stuffy and dry, so that the test is influenced.
SUMMERY OF THE UTILITY MODEL
The embodiment of the application provides a PCR laboratory with a dual-purpose airtight window, solves the technical problem that in the prior art, each area of the PCR laboratory is in a closed state for a long time and the energy consumption is large due to the fact that air supply of a purifying unit is adopted, achieves that each area of the PCR laboratory can naturally ventilate with the outside when a common PCR project without maintaining negative pressure is carried out, and greatly reduces energy consumption.
The embodiment of the present application provides a PCR laboratory with a dual-purpose airtight window, which includes:
an entry zone, the entry zone comprising:
a clean room;
the preparation room is communicated with the clean room through a door body;
the dressing room is communicated with the preparation room through a door body;
the special PCR walkway is communicated with the dressing room through a door body;
the reagent preparation area is communicated with the PCR special passage through a door body and is configured to prepare a storage reagent, split charging the reagent and prepare a main reaction mixed solution;
the reagent preparation area airtight window is arranged on one side wall of the reagent preparation area in an opening and closing mode, and when the reagent preparation area airtight window is opened, the reagent preparation area can naturally ventilate with the outside through the opening position of the reagent preparation area airtight window;
a reagent preparation area screen window openably disposed on the reagent preparation area at or on the reagent preparation area airtight window, the reagent preparation area screen window configured to shield an opening of the reagent preparation area airtight window when the reagent preparation area airtight window is opened;
the sample preparation area is communicated with the PCR special passage through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into an amplification reaction tube and synthesize cDNA;
the sample preparation area airtight window is arranged on one side wall of the sample preparation area in an opening and closing mode, and when the sample preparation area airtight window is opened, the sample preparation area can naturally ventilate with the outside through the opening position of the sample preparation area airtight window;
a sample preparation area screen openably disposed on the sample preparation area at or on the sample preparation area gas tight window, the sample preparation area screen configured to shield the sample preparation area gas tight window from opening when the sample preparation area gas tight window is opened;
a gene amplification region, wherein the gene amplification region is communicated with the PCR dedicated walkway through a gate body and is configured to amplify DNA or cDNA;
the gene amplification area airtight window can be arranged on one side wall of the gene amplification area in an opening and closing mode, and when the gene amplification area airtight window is opened, the gene amplification area can naturally ventilate with the outside through the opening position of the gene amplification area airtight window;
a gene amplification region screen window openably disposed on the gene amplification region and located at or openably disposed on the gene amplification region airtight window, the gene amplification region screen window configured to shield the gene amplification region airtight window from an opening position when the gene amplification region airtight window is opened;
a product analysis region, wherein the product analysis region is communicated with the PCR dedicated walkway through a gate body, and the product analysis region is configured to measure an amplified fragment;
the product analysis area airtight window can be arranged on one side wall of the product analysis area in an opening and closing mode, and when the product analysis area airtight window is opened, the product analysis area can naturally ventilate with the outside through the opening position of the product analysis area airtight window;
a product analysis region screen openably disposed on the product analysis region at or on the product analysis region gas seal, the product analysis region screen configured to shield the product analysis region gas seal from opening when the product analysis region gas seal is opened.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:
the PCR laboratory with the dual-purpose airtight window provided by the embodiment of the application can ensure the safety of natural ventilation by arranging the entrance area, the special passage for PCR, the reagent preparation area, the airtight window for the reagent preparation area, the screen window for the reagent preparation area, the sample preparation area, the airtight window for the sample preparation area, the screen window for the sample preparation area, the gene amplification area, the airtight window for the gene amplification area, the screen window for the gene amplification area, the product analysis area, the airtight window for the product analysis area and the screen window for the product analysis area, and by matching the airtight window for the reagent preparation area and the screen window for the reagent preparation area, the reagent preparation area can be in an airtight state after the airtight window for the reagent preparation area is closed, and by matching the airtight window for the sample preparation area and the screen window for the sample preparation area, after the airtight window of the sample preparation area is opened, the sample preparation area can naturally ventilate with the outside, and the safety of natural ventilation is ensured by shielding the airtight window of the sample preparation area, the sample preparation area can be in a closed state after the airtight window of the sample preparation area is closed, the airtight window of the gene amplification area and the screen window of the gene amplification area are matched, after the airtight window of the gene amplification area is opened, the gene amplification area can naturally ventilate with the outside, and the airtight window of the gene amplification area is shielded, so that the safety of natural ventilation is ensured, after the airtight window of the gene amplification area is closed, the gene amplification area can be in a closed state, through the matching of the airtight window of the product analysis area and the screen window of the product analysis area, after the airtight window of the product analysis area is opened, the product analysis area can naturally ventilate with the outside, and the screen window of the product analysis area is shielded, so that the safety of natural ventilation is ensured, after closing the product analysis area airtight window, can make the product analysis area in airtight state. Therefore, when a PCR project without maintaining negative pressure is carried out, the air-tight windows and the screen windows of all the zones can be enabled to naturally ventilate with the outside, air supply devices such as a purification unit and the like for supplying air can be omitted, energy consumption is effectively reduced, energy is saved, and the phenomenon that a tester is easily smoldered due to the fact that the tester is in a closed state for a long time can be avoided.
Drawings
The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
In the drawings:
FIG. 1 is a plan view of a PCR laboratory with a dual-purpose airtight window according to an embodiment of the present application;
FIG. 2 is a schematic view of the configuration of the reagent preparing area airtight window and the reagent preparing area screen window in cooperation according to an embodiment of the present disclosure;
FIG. 3 is a schematic structural view of a reagent preparation section airtight window provided in an embodiment of the present application in a closed state;
FIG. 4 is a schematic structural view of a reagent preparation area airtight window provided in an embodiment of the present application in an open state;
fig. 5 is a first schematic cross-sectional structural diagram of an outer frame of a first frame and a first window provided in an embodiment of the present application;
fig. 6 is a second schematic cross-sectional structural diagram of an outer frame of the first frame and the first window provided in the embodiment of the present application;
FIG. 7 is a schematic structural diagram of a sample preparation area hermetic window provided in an embodiment of the present application;
FIG. 8 is a schematic diagram showing the structure of a hermetic window of a gene amplification region provided in the examples of the present application;
fig. 9 is a schematic structural diagram of a product analysis area hermetic window provided in the embodiment of the present application.
In the figure:
100. a PCR laboratory with a dual-purpose airtight window; 10. entering a zone; 11. a clean room; 12. a preparation room; 13. a dressing room; 20. a PCR dedicated walkway; 30. a reagent preparation zone; 31. a reagent preparation buffer chamber; 32. a reagent preparation room; 33. a reagent preparation area airtight window; 34. a reagent preparation area screen window; 40. a sample preparation zone; 41. a sample preparation buffer room; 42. a sample preparation room; 43. a sample preparation area gas tight window; 44. a sample preparation area screen window; 50. a gene amplification region; 51. a gene amplification buffer; 52. during gene amplification; 53. a gene amplification region airtight window; 54. a gene amplification region screen window; 60. a product analysis zone; 61. a product analysis buffer; 62. a product analysis room; 63. a product analysis zone gas tight window; 64. a product analysis zone screen window; 331. a first frame body; 3310. the first frame body seals the stepped groove; 332. a first window; 3320. the first window body seals the stepped groove; 333. a first frame body seal strip; 334. a first window seal strip; 431. a second frame body; 432. a second window; 531. a third frame body; 532. a third window; 631. a fourth frame body; 632. and a fourth window.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
It is noted that, unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In the present invention, unless otherwise specified, the use of directional words such as "upper and lower" is generally in reference to the orientation shown in the drawings, or to the vertical, perpendicular or gravitational orientation; likewise, for ease of understanding and description, "left and right" are generally to the left and right as shown in the drawings; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself, but the above directional terms are not intended to limit the present invention.
In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In order to solve the technical problem that energy consumption is large due to the fact that in the prior art, all areas of a PCR laboratory are in a sealed state for a long time and air is supplied by a purifying unit, the embodiment of the application provides the PCR laboratory with a dual-purpose airtight window.
The present invention will be further explained with reference to the accompanying drawings.
As shown in fig. 1 to 9, the present embodiment provides a PCR laboratory with a dual-purpose airtight window, and a PCR laboratory 100 with a dual-purpose airtight window includes an entrance area 10, a PCR-dedicated walkway 20, a reagent preparation area 30, a reagent preparation area airtight window 33, a reagent preparation area screen 34, a sample preparation area 40, a sample preparation area airtight window 43, a sample preparation area screen 44, a gene amplification area 50, a gene amplification area airtight window 53, a gene amplification area screen 54, a product analysis area 60, a product analysis area airtight window 63, and a product analysis area screen 64.
The access area 10 includes a clean room 11, a preparation room 12, and a dressing room 13. The preparation room 12 is communicated with the clean room 11 through a door body, and the dressing room 13 is communicated with the preparation room 12 through a door body. The clean room 11 is provided with an entrance door for medical research and inspection personnel to enter, and then sequentially passes through the clean room 11, the preparation room 12 and the dressing room 13 to enter the PCR passage 20.
The PCR dedicated passage 20 is communicated with the dressing room 13 through a door body, and the PCR dedicated passage 20 is a common corridor which enters the reagent preparation area 30, the sample preparation area 40, the gene amplification area 50 and the product analysis area 60. The air pressure of the PCR exclusive path 20 may be +10 PA.
The reagent preparation area 30 is communicated with the PCR dedicated passage 20 through a door body, and the reagent preparation area 30 is configured to prepare a stored reagent, split-package the reagent, and prepare a main reaction mixed solution.
The reagent preparing area airtight window 33 is openably and closably disposed on a side wall of the reagent preparing area 30, and when the reagent preparing area airtight window 33 is opened, the reagent preparing area 30 can naturally ventilate with the outside through the opened position of the reagent preparing area airtight window 33.
A reagent preparation area screen 34 is openably disposed on the reagent preparation area 30 at the reagent preparation area airtight window 33 or openably disposed on the reagent preparation area airtight window 33, the reagent preparation area screen 34 being configured to shield the opening of the reagent preparation area airtight window 33 when the latter is opened.
The sample preparation area 40 is communicated with the PCR dedicated passage 20 through a door body, and the sample preparation area 40 is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA.
The sample preparation area air-tight window 43 is openably and closably disposed on one side wall of the sample preparation area 40, and when the sample preparation area air-tight window 43 is opened, the sample preparation area 40 can naturally ventilate with the outside through the opened portion of the sample preparation area air-tight window 43.
A sample preparation area screen 44 is openably disposed on the sample preparation area 40 at the sample preparation area air-tight window 43 or openably disposed on the sample preparation area air-tight window 43, the sample preparation area screen 44 being configured to shield the sample preparation area air-tight window 43 from opening when the window is opened.
The gene amplification region 50 is communicated with the PCR dedicated walkway 20 through a gate body, and the gene amplification region 50 is configured to amplify DNA or cDNA.
The gene amplification region airtight window 53 is openably and closably provided on one side wall of the gene amplification region 50, and when the gene amplification region airtight window 53 is opened, the gene amplification region 50 can naturally ventilate with the outside through the opened portion of the gene amplification region airtight window 53.
The gene amplification region screen 54 is openably disposed on the gene amplification region 50 at the gene amplification region airtight window 53 or openably disposed on the gene amplification region airtight window 53, and the gene amplification region screen 54 is configured to shield the opening of the gene amplification region airtight window 53 when the same is opened.
The product analysis region 60 is communicated with the PCR dedicated passage 20 through a gate body, and the product analysis region 60 is configured to measure an amplified fragment.
The product analysis region air-tight window 63 is openably and closably disposed on a sidewall of the product analysis region 60, and when the product analysis region air-tight window 63 is opened, the product analysis region 60 can naturally ventilate with the outside through the opened portion of the product analysis region air-tight window 63.
A product analysis zone screen 64 is openably disposed on the product analysis zone 60 at the product analysis zone gas seal 63 or openably disposed on the product analysis zone gas seal 63, the product analysis zone screen 64 being configured to block the opening of the product analysis zone gas seal 63 when the latter is opened.
According to the PCR laboratory with the dual-purpose airtight window provided by the embodiment of the application, the entrance area 10, the special PCR walkway 20, the reagent preparation area 30, the reagent preparation area airtight window 33, the reagent preparation area screen window 34, the sample preparation area 40, the sample preparation area airtight window 43, the sample preparation area screen window 44, the gene amplification area 50, the gene amplification area airtight window 53, the gene amplification area screen window 54, the product analysis area 60, the product analysis area airtight window 63 and the product analysis area screen window 64 are arranged, through the cooperation of the reagent preparation area airtight window 33 and the reagent preparation area screen window 34, after the reagent preparation area airtight window 33 is opened, the reagent preparation area 30 can naturally ventilate with the outside, and the reagent preparation area 34 shields the reagent preparation area airtight window, so that the entrance of external arthropods, mice and the like can be prevented, the safety of natural ventilation can be ensured, and the reagent preparation area 30 can be in an airtight state after the reagent preparation area airtight window 33 is closed, by the cooperation of the sample preparation area airtight window 43 and the sample preparation area screen window 44, the sample preparation area 40 can be naturally ventilated with the outside after the sample preparation area airtight window 43 is opened, and the safety of natural ventilation is ensured by shielding the sample preparation area screen window 44, the sample preparation area 40 can be in a closed state after the sample preparation area airtight window 43 is closed, the gene amplification area 50 can be naturally ventilated with the outside by the cooperation of the gene amplification area airtight window 53 and the gene amplification area screen window 54 after the gene amplification area airtight window 53 is opened, and the safety of natural ventilation can be ensured by shielding the gene amplification area screen window 54, the gene amplification area 50 can be in a closed state after the gene amplification area airtight window 53 is closed, the product analysis area airtight window 63 and the product analysis area screen window 64 can be engaged, after the product analysis area airtight window 63 is opened, the product analysis area 60 can naturally ventilate with the outside, and the product analysis area is shielded by the product analysis area screen 64, so that the safety of the natural ventilation is ensured, and the product analysis area 60 can be in a sealed state after the product analysis area air-tight window 63 is closed. Therefore, when a PCR project without maintaining negative pressure is carried out, the air-tight windows and the screen window of each region can be naturally ventilated with the outside through the air-tight windows and the screen window, an air supply device such as a purification unit for supplying air can be omitted, energy consumption is effectively reduced, energy is saved, when the PCR project is carried out in a negative pressure state, the air-tight windows of each region are closed, dual purposes are realized, and the method is suitable for carrying out PCR projects in special periods such as common PCR projects and epidemic situations.
In this embodiment, the reagent preparing section 30 includes a reagent preparing buffer compartment 31 and a reagent preparing compartment 32.
The reagent preparation buffer room 31 is communicated with the special PCR passage 20 through a door body, and the reagent preparation buffer room 31 is a buffer space entering the reagent preparation room 32 from the special PCR passage 20. The air pressure of the reagent preparation buffer compartment 31 may be 0 PA.
The reagent preparation room 32 is communicated with the reagent preparation buffer room 31 through a door body, and the reagent preparation room 32 is configured to prepare a stored reagent, split the reagent and prepare a main reaction mixed solution. The reagents and materials for specimen preparation are directly transported to the reagent preparing room 32, and the reagent raw materials are stored in the reagent preparing room 32. The reagent preparation room 32 can be equipped with a 2-8 ℃ refrigerator and a-80 ℃ refrigerator. The area of the room may be 15m2～20m2. The pressure gradient of the reagent preparation chamber 32 may be in a relatively positive pressure state (may be +10PA) to prevent the outside air containing nucleic acid aerosol from entering and causing pollution. A reagent preparation room air conditioner is provided in the reagent preparation room 32, and is configured to adjust the temperature and humidity inside the reagent preparation room.
Wherein, the reagent preparing area airtight window 33 is openably and closably disposed on a sidewall of the reagent preparing room 32 away from the reagent preparing buffer room 31, and the reagent preparing area screen 34 is openably disposed on the reagent preparing room 32 at the reagent preparing area airtight window 33 or openably disposed on the reagent preparing area airtight window 33. When the air-tight window 33 of the reagent preparation area is opened, the reagent preparation room can be communicated with the outside and naturally ventilate, and when the air-tight window is closed, the reagent preparation room is in a closed state.
In this embodiment, the sample preparation area 40 includes a sample preparation buffer compartment 41 and a sample preparation compartment 42.
The sample preparation buffer room 41 is communicated with the PCR special passage 20 through a door body. The sample preparation buffer room 41 is a buffer space that enters the sample preparation room 42 from the PCR-dedicated lane 20. The air pressure of the sample preparation buffer 41 may be 0 PA.
The sample preparation room 42 is communicated with the sample preparation buffer room 41 through a door body, and the sample preparation room 42 is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA. The sample preparation room 42 may be equipped with a 2-8 ℃ refrigerator and a-20 ℃ refrigerator. The sample preparation room 42 is equipped with a biosafety cabinet for performing operations for extracting nucleic acids. To avoid repeated cycling of the extracted nucleic acids within the cabinet, resulting in cross-contamination between samples, and false positive results, the sample preparation booth 42 must be equipped with a biosafety cabinet of type B2. The vertical air flow of the working area of the biological safety cabinet comes from a laboratory, and the exhausted air is directly exhausted to the outside after being filtered by the high-efficiency filter and is not allowed to return to the safety cabinet and the laboratory. The area of the sample preparation compartment 42 may be 25m2～30m2In the meantime. The pressure gradient in the sample preparation bay 42 is desirably positive with respect to the adjacent area (which may be-10 PA) to avoid aerosol contamination from the adjacent area into the local area. A sample preparation room air conditioner is disposed in the sample preparation room 42 and configured to adjust the temperature and humidity inside the sample preparation room.
Wherein, the sample preparation area airtight window 43 is openably and closably disposed on a sidewall of the sample preparation room 42 away from the sample preparation buffer room 41, and the sample preparation area screen 44 is openably disposed on the sample preparation room 42 at the sample preparation area airtight window 43 or openably disposed on the sample preparation area airtight window 43. The sample preparation section air-tight window 43 allows the sample preparation compartment to communicate with the outside when opened, and allows the sample preparation compartment to be closed when closed.
In this embodiment, the gene amplification region 50 includes a gene amplification buffer room 51 and a gene amplification room 52.
The gene amplification buffer room 51 is communicated with the PCR special passage 20 through a door body. The gene amplification buffer room 51 is a buffer space which enters the gene amplification room 52 from the PCR dedicated path 20. The gas pressure in the gene amplification buffer 51 may be 0 PA.
The gene amplification room 52 communicates with the gene amplification buffer room 51 through a gate body, and the gene amplification room 52 is configured to amplify DNA or cDNA. In addition, D has been preparedThe addition of the NA template and synthesized cDNA (from the sample preparation room 42) and the preparation of the main reaction mixture (from the reagent preparation room 32) into a reaction mixture, etc. may also be performed in the gene amplification room 52. In nested PCR assays, the reaction tube must usually be opened after the first round of amplification, so nested amplification has a high risk of contamination, and the second sample addition must be performed in the gene amplification chamber 52. The gene amplification room 52 is equipped with the core instrument of the PCR laboratory: as the PCR instrument, for example, two types of PCR instruments, ABI7500 and ABI9700, can be used. The area of the gene amplification chamber 52 can be controlled to be 15m2～20m2. The pressure gradient of the gene amplification chamber 52 is required to be negative pressure with respect to the adjacent region (the gene amplification chamber 52 may be-15 PA) to avoid the escape of aerosol from the region. To avoid contamination by aerosols, unnecessary movement within the region should be minimized. The gene amplification room 52 is provided with a gene amplification room air conditioner configured to regulate the temperature and humidity inside the gene amplification room 52.
Wherein, the gene amplification region airtight window 53 is openably and closably disposed on a side wall of the gene amplification chamber 52 away from the gene amplification buffer chamber 51, and the gene amplification region screen 54 is openably disposed on the gene amplification chamber 52 at the gene amplification region airtight window 53 or openably disposed on the gene amplification region airtight window 53. When the airtight window 53 of the gene amplification region is opened, the gene amplification chamber 52 can communicate with the outside to ventilate naturally, and when closed, the gene amplification chamber 52 is in a closed state.
In this embodiment, product analysis zone 60 includes a product analysis buffer compartment 61 and a product analysis compartment 62.
The product analysis buffer room 61 is communicated with the PCR special passage 20 through a door body. The product analysis buffer room 61 is a buffer channel from the PCR dedicated walkway 20 into the product analysis room 62. The pressure in product analysis buffer 61 may be 0 PA.
The product analysis room 62 communicates with the product analysis buffer room 61 through a door, and the product analysis room 62 is configured to measure the amplified fragment. The area of the product analysis chamber 62 can be controlled to be 15m2～20m2. The product analysis chamber 62 is the most important source of contamination of the amplification product, and therefore the pressure gradient in this region is required to be negative with respect to the adjacent region(the product analysis compartment 62 may be-20 PA) to avoid diffusion of amplification products from this region to other regions. A product analysis room air conditioner is provided in the product analysis room 62, and is configured to adjust the temperature and humidity inside the product analysis room 62.
Wherein, the product analysis region air-tight window 63 can be openably and closably disposed on a sidewall of the product analysis chamber 62 away from the product analysis buffer chamber 61, and the product analysis region screen 64 can be openably disposed on the product analysis chamber 62 and located at the product analysis region air-tight window 63 or openably disposed on the product analysis region air-tight window 63. When the product analysis area airtight window 63 is opened, the product analysis room 62 can be communicated with the outside and naturally ventilate, and when the product analysis room 62 is closed, the product analysis room is in a closed state.
In this embodiment, the reagent preparation area airtight window 33 includes a first frame 331, a first window 332, a first frame sealing strip 333, and a first window sealing strip 334.
The first frame body 331 is disposed on one side wall of the reagent preparing section 30, and a first frame body sealing stepped groove 3310 is circumferentially provided on an inner side of the first frame body 331, and the first frame body sealing stepped groove is stepped.
The first window 332 is hinged to the first frame 331 to close the first frame 331, a first window sealing stepped groove 3320 is formed in the outer periphery of the first window 332, and the first window sealing stepped groove is matched with the first frame sealing stepped groove.
The first frame sealing strip 333 is disposed in the first frame sealing stepped groove, and the first frame sealing strip 333 may be made of rubber.
First window body sealing strip 334 sets up in the sealed ladder groove of first window body, and first window body sealing strip 334 can be the rubber material.
Here, when the first window 332 closes the first frame 331, the first frame seal 333 and the first window seal 334 are in press contact to seal a gap between the first window 332 and the first frame 331.
With the above arrangement, the airtightness when the reagent preparing section airtight window 33 is closed can be improved.
In this embodiment, the sample preparation area airtight window 43 includes a second frame 431, a second window 432, a second frame seal, and a second window seal.
The second frame body 431 is disposed on one sidewall of the sample preparation area 40, and a second frame body sealing stepped groove is circumferentially formed on the inner side of the second frame body 431, and the second frame body sealing stepped groove is stepped.
The second window body 432 is hinged to the second frame body 431 to be used for closing the second frame body 431, a second window body sealing stepped groove is formed in the outer edge of the second window body 432 in the circumferential direction, and the second window body sealing stepped groove is matched with the second frame body sealing stepped groove.
The second frame body sealing strip is arranged in the second frame body sealing stepped groove.
And the second window body sealing strip is arranged in the second window body sealing stepped groove.
When the second window 432 closes the second frame 431, the second frame seal and the second window seal are in pressing contact to seal a gap between the second window 432 and the second frame 431.
With the above arrangement, the airtightness when the sample preparation area airtight window 43 is closed can be improved.
In this embodiment, the gene amplification region airtight window 53 includes a third frame 531, a third window 532, a third frame seal, and a third window seal.
The third frame body 531 is disposed on one side wall of the gene amplification region 50, and a third frame body sealing stepped groove is formed in the inner circumferential direction of the third frame body 531.
The third window body 532 is hinged to the third frame body 531 to be used for closing the third frame body 531, a third window body sealing stepped groove is formed in the outer edge of the third window body 532 in the circumferential direction, and the third window body sealing stepped groove is matched with the third frame body sealing stepped groove.
The third frame body sealing strip is arranged in the third frame body sealing stepped groove.
The third window body sealing strip is arranged in the third window body sealing stepped groove.
When the third window 532 closes the third frame 531, the third frame weatherstrip and the third window weatherstrip are in pressing contact to seal a gap between the third window 532 and the third frame 531.
With the above arrangement, the airtightness of the airtight window 53 for gene amplification region when closed can be improved.
In this embodiment, the product analysis area airtight window 63 includes a fourth frame 631, a fourth window 632, a fourth frame sealing strip, and a fourth window sealing strip.
The fourth frame 631 is disposed on a sidewall of the product analysis region 60, and a fourth frame sealing stepped groove is formed in an inner circumferential direction of the fourth frame 631.
The fourth window body 632 is hinged to the fourth frame body 631 to close the fourth frame body 631, a fourth window body sealing stepped groove is formed in the circumferential direction of the outer edge of the fourth window body 632, and the fourth window body sealing stepped groove is matched with the fourth frame body sealing stepped groove.
The fourth frame body sealing strip is arranged in the fourth frame body sealing stepped groove.
The fourth window sealing strip is arranged in the fourth window sealing stepped groove.
When the fourth window 632 closes the fourth frame 631, the fourth frame seal and the fourth window seal are in pressing contact to seal a gap between the fourth window 632 and the fourth frame 631.
With the above arrangement, the airtightness when the product analysis region airtight window 63 is closed can be improved.
The reagent preparation area air-tight window 33, the sample preparation area air-tight window 43, the gene amplification area air-tight window 53, and the product analysis area air-tight window 63 may have the same structure. The structure is not limited to the above structure, and other airtight window structures in the prior art may be adopted.
In this embodiment, the reagent preparation area screen 34, the sample preparation area screen 44, the gene amplification area screen 54, and the product analysis area screen 64 may be all retractable roll screens, and may include a screen frame, a screen mesh disposed on the top of the screen frame and automatically retractable into the roll, and a screen mesh (which may be a glass fiber screen) slidably disposed on the inner side of the screen frame, where the screen frame may be fixed on the side wall of each area or directly fixed on the frame of the corresponding airtight window.
It should be noted that the configurations of the reagent preparation area screen 34, the sample preparation area screen 44, the gene amplification area screen 54, and the product analysis area screen 64 are not limited thereto, and alternatively, they may be screens with adhesion, such as magnetic adhesion.
In this embodiment, the PCR laboratory with the dual-purpose airtight window may further include a reagent preparation area independent air exhaust device and a reagent preparation area independent air supply device.
The reagent preparation area independent exhaust device is configured to exhaust air in the reagent preparation room to the outside of the reagent preparation room.
The reagent preparation area independent air supply device is configured to supply air into the reagent preparation room.
In this embodiment, the PCR laboratory with the dual-purpose airtight window may further include a sample preparation area independent air exhaust device and a sample preparation area independent air supply device.
The sample preparation zone independent exhaust device is configured to exhaust air in the sample preparation compartment to an exterior of the sample preparation compartment.
The sample preparation area independent air supply device is configured to supply air into the sample preparation room.
In this embodiment, the PCR laboratory with the dual-purpose airtight window may further include a gene amplification region independent air exhaust device and a gene amplification region independent air supply device.
The gene amplification region independent air discharge device is configured to discharge air in the gene amplification chamber to the outside of the gene amplification chamber.
The gene amplification region independent air blowing means is configured to blow air into the gene amplification chamber.
In this embodiment, the PCR laboratory with the dual-purpose airtight window may further include a product analysis area independent air exhaust device and a product analysis area independent air supply device.
The product analysis zone independent exhaust is configured to exhaust air in the product analysis compartment to the exterior of the product analysis compartment.
The product analysis zone independent air supply device is configured to supply air into the product analysis chamber.
The independent exhaust device of the reagent preparation area, the independent exhaust device of the sample preparation area, the independent exhaust device of the gene amplification area and the independent exhaust device of the product analysis area are independent of each other and are not communicated with each other, the independent exhaust device of the reagent preparation area and the independent air supply device of the reagent preparation area are independent of each other and are not communicated with each other, the independent exhaust device of the sample preparation area and the independent air supply device of the sample preparation area are independent of each other and are not communicated with each other, the independent exhaust device of the product analysis area and the independent air supply device of the product analysis area are independent of each other and are not communicated with each other, and therefore pollution is prevented among reagent preparation, sample preparation, gene amplification and product analysis.
The PCR laboratory with the dual-purpose airtight window comprises a first state and a second state, wherein in the first state, the reagent preparation area airtight window 33, the sample preparation area airtight window 43, the gene amplification area airtight window 53 and the product analysis area airtight window 63 are all opened, so that the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52 and the product analysis room 62 are respectively communicated with the outside, and the reagent preparation area independent air supply device, the sample preparation area independent air supply device, the gene amplification area independent air supply device and the product analysis area independent air supply device are all in a stop state; not only can guarantee good independent ventilation, and can need not to set up and purify air conditioning unit, open exhaust device can, the energy can be saved and need not extravagant space and settle and purify air conditioning unit. In the second state, the reagent preparation area airtight window 33, the sample preparation area airtight window 43, the gene amplification area airtight window 53, and the product analysis area airtight window 63 are all closed, so that the reagent preparation room 32, the sample preparation room 42, the gene amplification room 52, and the product analysis room 62 are respectively in an airtight state, and the reagent preparation area independent air exhaust device, the sample preparation area independent air exhaust device, the gene amplification area independent air exhaust device, the product analysis area independent air exhaust device, the reagent preparation area independent air supply device, the sample preparation area independent air supply device, the gene amplification area independent air supply device, and the product analysis area independent air supply device are all in a working state. Air supply and air exhaust of each area are respectively and independently carried out, the safety performance of each area is effectively guaranteed, and the method is extremely suitable for epidemic situation PCR projects.
In the present embodiment, the reagent preparing section independent air exhausting device is configured to simultaneously exhaust the air in the reagent preparing compartment 32 and the air in the reagent preparing buffer compartment 31 to the outside.
The independent exhaust device of the reagent preparation area can comprise a main independent exhaust pipeline of the reagent preparation area, an independent exhaust fan of the reagent preparation area, an efficient exhaust filter of the reagent preparation area, an exhaust branch pipe of the reagent preparation buffer room and an exhaust branch pipe of the reagent preparation room.
The main independent air exhaust pipeline of the reagent preparation area is arranged in the reagent preparation room 32, and one end of the main independent air exhaust pipeline of the reagent preparation area is communicated with the outside, namely the air exhaust end.
The reagent preparation area independent exhaust fan is arranged on the reagent preparation area independent exhaust main pipeline and configured to provide exhaust power.
The reagent preparation area air exhaust high-efficiency filter is arranged on the reagent preparation area independent air exhaust main pipeline and is configured to filter exhausted air and prevent harmful substances and germs from being exhausted. The reagent preparation area air exhaust high-efficiency filter can be arranged on the air exhaust side of the reagent preparation area independent exhaust fan on the reagent preparation area independent air exhaust main pipeline.
One end of the air exhaust branch pipe of the reagent preparation buffering room is communicated with the main independent air exhaust pipe of the reagent preparation area, and the other end of the air exhaust branch pipe of the reagent preparation buffering room is communicated with the reagent preparation buffering room 31.
One end of the exhaust branch pipe of the reagent preparation room is communicated with the independent exhaust main pipeline of the reagent preparation area, and the other end of the exhaust branch pipe of the reagent preparation room is communicated with the reagent preparation room 32.
Through the work of the independent exhaust fan in the reagent preparation area, the air in the reagent preparation buffer room 31 enters the main independent exhaust pipe in the reagent preparation area through the exhaust branch pipe in the reagent preparation buffer room, the air in the reagent preparation room 32 enters the main independent exhaust pipe in the reagent preparation area through the exhaust branch pipe in the reagent preparation room, the independent exhaust fan in the reagent preparation area exhausts the air to the efficient exhaust filter in the reagent preparation area, and the air is exhausted to the outside after being filtered by the efficient exhaust filter in the reagent preparation area. The simultaneous discharge of the air in the reagent preparing compartment 32 and the air in the reagent preparing buffer compartment 31 is achieved.
The structures of the independent air exhaust device in the sample preparation area, the independent air exhaust device in the gene amplification area and the independent air exhaust device in the product analysis area can adopt the same or similar structures with the independent air exhaust device in the reagent preparation area, and the detailed description is omitted here.
In this embodiment, the reagent preparing section independent air blowing means is configured to simultaneously blow air into the reagent preparing compartment 32 and the reagent preparing buffer compartment 31.
The independent air supply device of the reagent preparation area comprises a main independent air supply pipeline of the reagent preparation area, an air supply branch pipe of the reagent preparation buffer room, an independent air supply machine of the reagent preparation area and an air supply filter of the reagent preparation area.
The main independent air supply pipeline of the reagent preparation area penetrates through the reagent preparation buffer room 31 and the reagent preparation room 32, one end of the main independent air supply pipeline of the reagent preparation area is communicated with the outside, and the other end of the main independent air supply pipeline of the reagent preparation area is communicated with the reagent preparation room 32.
One end of the reagent preparation buffer room air supply branch pipe is communicated with the reagent preparation area independent air supply main pipeline, and the other end of the reagent preparation buffer room air supply branch pipe is communicated with the reagent preparation buffer room 31.
The independent blower of the reagent preparation area is arranged on the main independent blowing pipeline of the reagent preparation area and is configured to provide blowing power.
The reagent preparation area air supply filter is arranged on the reagent preparation area independent air supply main pipeline and is configured to filter air sent into the reagent preparation room 32 and the reagent preparation buffer room 31. The reagent preparation area air supply filter can be a primary filter or a high-efficiency filter.
The reagent preparation area independent air feeder provides power, air is led to the reagent preparation area air feeding filter, filtered by the reagent preparation area air feeding filter and enters the reagent preparation area independent air feeding main pipeline, the reagent preparation area independent air feeding main pipeline is discharged into the reagent preparation buffering room through the reagent preparation buffering room air feeding branch pipe, and meanwhile, one end of the reagent preparation area independent air feeding main pipeline, which is positioned in the reagent preparation room 32, feeds air into the reagent preparation room 32. Thereby realizing the introduction of fresh air.
The structures of the independent air supply device in the sample preparation area, the independent air supply device in the gene amplification area and the independent air supply device in the product analysis area can be the same as or similar to the structure of the independent air supply device in the reagent preparation area, and are not repeated herein.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the creative work should be covered within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.
Claims (9)
1. A PCR laboratory having a dual-purpose hermetic window, the PCR laboratory having the dual-purpose hermetic window comprising:
an entry zone, the entry zone comprising:
a clean room;
the preparation room is communicated with the clean room through a door body;
the dressing room is communicated with the preparation room through a door body;
the special PCR walkway is communicated with the dressing room through a door body;
the reagent preparation area is communicated with the PCR special passage through a door body and is configured to prepare a storage reagent, split charging the reagent and prepare a main reaction mixed solution;
the reagent preparation area airtight window is arranged on one side wall of the reagent preparation area in an opening and closing mode, and when the reagent preparation area airtight window is opened, the reagent preparation area can naturally ventilate with the outside through the opening position of the reagent preparation area airtight window;
a reagent preparation area screen window openably disposed on the reagent preparation area at or on the reagent preparation area airtight window, the reagent preparation area screen window configured to shield an opening of the reagent preparation area airtight window when the reagent preparation area airtight window is opened;
the sample preparation area is communicated with the PCR special passage through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into an amplification reaction tube and synthesize cDNA;
the sample preparation area airtight window is arranged on one side wall of the sample preparation area in an opening and closing mode, and when the sample preparation area airtight window is opened, the sample preparation area can naturally ventilate with the outside through the opening position of the sample preparation area airtight window;
a sample preparation area screen openably disposed on the sample preparation area at or on the sample preparation area gas tight window, the sample preparation area screen configured to shield the sample preparation area gas tight window from opening when the sample preparation area gas tight window is opened;
a gene amplification region, wherein the gene amplification region is communicated with the PCR dedicated walkway through a gate body and is configured to amplify DNA or cDNA;
the gene amplification area airtight window can be arranged on one side wall of the gene amplification area in an opening and closing mode, and when the gene amplification area airtight window is opened, the gene amplification area can naturally ventilate with the outside through the opening position of the gene amplification area airtight window;
a gene amplification region screen window openably disposed on the gene amplification region and located at or openably disposed on the gene amplification region airtight window, the gene amplification region screen window configured to shield the gene amplification region airtight window from an opening position when the gene amplification region airtight window is opened;
a product analysis region, wherein the product analysis region is communicated with the PCR dedicated walkway through a gate body, and the product analysis region is configured to measure an amplified fragment;
the product analysis area airtight window can be arranged on one side wall of the product analysis area in an opening and closing mode, and when the product analysis area airtight window is opened, the product analysis area can naturally ventilate with the outside through the opening position of the product analysis area airtight window;
a product analysis region screen openably disposed on the product analysis region at or on the product analysis region gas seal, the product analysis region screen configured to shield the product analysis region gas seal from opening when the product analysis region gas seal is opened.
2. The PCR laboratory having a dual-purpose airtight window according to claim 1, wherein said reagent preparation region comprises:
the reagent preparation buffer room is communicated with the PCR special passage through a door body; and
the reagent preparation room is communicated with the reagent preparation buffer room through a door body and is configured to prepare a stored reagent, split-package the reagent and prepare a main reaction mixed solution;
the reagent preparation area air-tight window can be arranged on one side wall of the reagent preparation room far away from the reagent preparation buffer room in an opening and closing mode, and the reagent preparation area screen window is arranged on the reagent preparation room in an opening mode and located at the reagent preparation area air-tight window or arranged on the reagent preparation area air-tight window in an opening mode.
3. The PCR laboratory with a dual-purpose hermetic window of claim 1, wherein the sample preparation zone comprises:
the sample preparation buffer room is communicated with the PCR special passage through a door body; and
the sample preparation room is communicated with the sample preparation buffer room through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into the amplification reaction tube and synthesize cDNA;
the sample preparation area airtight window is openably and closably arranged on one side wall of the sample preparation room far away from the sample preparation buffer room, and the sample preparation area screen window is openably arranged on the sample preparation room and positioned at the sample preparation area airtight window or openably arranged on the sample preparation area airtight window.
4. The PCR laboratory with dual-purpose hermetic window of claim 1, wherein the gene amplification region comprises:
the gene amplification buffer room is communicated with the PCR special walkway through a door body; and
a gene amplification chamber, wherein the gene amplification chamber is communicated with the gene amplification buffer chamber through a door body, and the gene amplification chamber is configured to amplify DNA or cDNA;
the gene amplification area air-tight window can be arranged on one side wall of the gene amplification room far away from the gene amplification buffer room in an opening and closing mode, and the gene amplification area screen window can be arranged on the gene amplification room in an opening mode and is positioned at the gene amplification area air-tight window or can be arranged on the gene amplification area air-tight window in an opening mode.
5. The PCR laboratory with dual-purpose hermetic window according to claim 1, wherein said product analysis zone comprises:
a product analysis buffer room communicated with the PCR special passage through a door body; and
a product analysis room, wherein the product analysis room is communicated with the product analysis buffer room through a door body, and the product analysis room is configured to measure the amplified fragments;
the product analysis area airtight window can be arranged on one side wall of the product analysis room far away from the product analysis buffer room in an opening and closing mode, and the product analysis area screen window is arranged on the product analysis room in an opening mode and located at the product analysis area airtight window or arranged on the product analysis area airtight window in an opening mode.
6. The PCR laboratory having a dual-purpose hermetic window according to claim 1, wherein the reagent preparation section hermetic window comprises:
the first frame body is arranged on one side wall of the reagent preparation area, and a first frame body sealing stepped groove is formed in the circumferential direction of the inner side of the first frame body;
the first window body is hinged to the first frame body and used for closing the first frame body, a first window body sealing stepped groove is formed in the circumferential direction of the outer edge of the first window body, and the first window body sealing stepped groove is matched with the first frame body sealing stepped groove;
the first frame body sealing strip is arranged in the first frame body sealing stepped groove;
the first window body sealing strip is arranged in the first window body sealing stepped groove;
when the first window body seals the first frame body, the first frame body sealing strip and the first window body sealing strip are in pressing contact to seal a gap between the first window body and the first frame body.
7. The PCR laboratory having a dual-purpose hermetic window according to claim 1, wherein said sample preparation section hermetic window comprises:
the second frame body is arranged on one side wall of the sample preparation area, and a second frame body sealing stepped groove is formed in the circumferential direction of the inner side of the second frame body;
the second window body is hinged to the second frame body and used for closing the second frame body, a second window body sealing stepped groove is formed in the outer edge of the second window body in the circumferential direction, and the second window body sealing stepped groove is matched with the second frame body sealing stepped groove;
the second frame body sealing strip is arranged in the second frame body sealing stepped groove;
the second window body sealing strip is arranged in the second window body sealing stepped groove;
when the second window body seals the second frame body, the second frame body sealing strip and the second window body sealing strip are in pressing contact to seal a gap between the second window body and the second frame body.
8. The PCR laboratory having a dual-purpose hermetic window according to claim 1, wherein the gene amplification region hermetic window comprises:
the third frame body is arranged on one side wall of the gene amplification region, and a third frame body sealing stepped groove is formed in the circumferential direction of the inner side of the third frame body;
the third window body is hinged to the third frame body and used for closing the third frame body, a third window body sealing stepped groove is formed in the outer edge of the third window body in the circumferential direction, and the third window body sealing stepped groove is matched with the third frame body sealing stepped groove;
the third frame body sealing strip is arranged in the third frame body sealing stepped groove;
the third window body sealing strip is arranged in the third window body sealing stepped groove;
when the third window body closes the third frame body, the third frame body sealing strip and the third window body sealing strip are in pressing contact to seal a gap between the third window body and the third frame body.
9. The PCR laboratory having a dual-purpose hermetic window according to claim 1, wherein said product analysis region hermetic window comprises:
the fourth frame body is arranged on one side wall of the product analysis area, and a fourth frame body sealing stepped groove is formed in the circumferential direction of the inner side of the fourth frame body;
the fourth window body is hinged to the fourth frame body and used for closing the fourth frame body, a fourth window body sealing stepped groove is formed in the outer edge of the fourth window body in the circumferential direction, and the fourth window body sealing stepped groove is matched with the fourth frame body sealing stepped groove;
the fourth frame body sealing strip is arranged in the fourth frame body sealing stepped groove;
the fourth window body sealing strip is arranged in the fourth window body sealing stepped groove;
when the fourth window body closes the fourth frame body, the fourth frame body sealing strip and the fourth window body sealing strip are in pressing contact to seal a gap between the fourth window body and the fourth frame body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021435957.6U CN212743517U (en) | 2020-07-20 | 2020-07-20 | PCR laboratory with dual-purpose airtight window |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021435957.6U CN212743517U (en) | 2020-07-20 | 2020-07-20 | PCR laboratory with dual-purpose airtight window |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212743517U true CN212743517U (en) | 2021-03-19 |
Family
ID=75037294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021435957.6U Active CN212743517U (en) | 2020-07-20 | 2020-07-20 | PCR laboratory with dual-purpose airtight window |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212743517U (en) |
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2020
- 2020-07-20 CN CN202021435957.6U patent/CN212743517U/en active Active
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