CN212538176U - Dual-purpose ventilation system PCR laboratory - Google Patents

Dual-purpose ventilation system PCR laboratory Download PDF

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Publication number
CN212538176U
CN212538176U CN202021425317.7U CN202021425317U CN212538176U CN 212538176 U CN212538176 U CN 212538176U CN 202021425317 U CN202021425317 U CN 202021425317U CN 212538176 U CN212538176 U CN 212538176U
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room
air
area
gene amplification
air supply
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曾黎峰
万林静
李蓉
黄清
夏晓健
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Jiangxi Provincial Peoples Hospital
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Jiangxi Provincial Peoples Hospital
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Abstract

The utility model discloses a dual-purpose ventilation system PCR laboratory belongs to PCR laboratory field, and dual-purpose ventilation system PCR laboratory includes: entering a zone; a PCR dedicated walkway; a reagent preparation zone; a sample preparation zone; a gene amplification region; a product analysis zone; an air exhaust device; air supply arrangement includes: the air inlet end of the air generating part is not communicated with the air exhaust end of the air exhaust device; the air supply main pipeline is communicated with the air outlet end of the air generating part; the output end of the reagent preparation area air supply pipe extends to the reagent preparation area; the output end of the sample preparation area blast pipe extends to the sample preparation area; the output end of the gene amplification region air supply pipe extends to the gene amplification region; and the output end of the product analysis area blast pipe extends to the product analysis area. The utility model discloses a dual-purpose type ventilation system PCR laboratory has realized making between each district of PCR laboratory relatively independent ventilation, avoids the possibility that the cross contamination condition appears.

Description

Dual-purpose ventilation system PCR laboratory
Technical Field
The utility model relates to a PCR laboratory field especially relates to a dual-purpose ventilation system PCR laboratory.
Background
The PCR laboratory is also called a gene amplification laboratory, and the PCR laboratory generally comprises a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area. PCR is a short term for Polymerase Chain Reaction (Polymerase Chain Reaction), a molecular biology technique used to amplify specific DNA fragments and can be used as a special DNA copy in vitro. The virus content in the body of a patient can be rapidly mastered by a DNA gene tracking system, and the accuracy of the system reaches the nanometer level. For example, the number of hepatitis B viruses in a patient body, whether the hepatitis B viruses are duplicated or not, whether the hepatitis B viruses are infected or not, how strong the infection exists, whether the hepatitis B viruses need to be taken or not, whether the liver functions are abnormally changed or not can be accurately detected, which antiviral drugs are most suitable for the patient to use can be judged in time, and the curative effect of the drugs can be judged, so that a reliable inspection basis is provided for clinical treatment.
In the process of implementing the embodiments of the present invention, the inventor finds that at least the following defects exist in the background art:
each area of PCR laboratory all needs to ventilate, and in current PCR laboratory, it all adopts the mode of circulation ventilation, be about to discharge the air in each area and get into each area once more after the air conditioning unit in order to reuse air temperature and energy-conservation, however, circulation ventilation leads to the air current can't absolutely keep apart, easily makes air cross contamination, not only leads to the air that pollutes to circulate in each interval of PCR laboratory, seriously threatens medical research and inspector's health, the security performance is low, and can pollute test material such as reagent, sample, lead to the testing result to be out of alignment. Especially, in the period of resisting epidemic situations such as Severe Acute Respiratory Syndrome (SARS), avian influenza infection of people, Middle East Respiratory Syndrome (MERS), novel coronavirus pneumonia (COVID-19) and the like, the problems are aggravated due to strong infectivity, and the common PCR laboratory is disabled.
SUMMERY OF THE UTILITY MODEL
The embodiment of the application provides a dual-purpose ventilation system PCR laboratory, solves the technical problems that the health of medical research and inspection personnel is easily threatened and the detection result is inaccurate due to the adoption of a circulating ventilation mode among all the areas of the PCR laboratory in the prior art, realizes relatively independent ventilation among all the areas of the PCR laboratory, and avoids the possibility of cross contamination.
The embodiment of the present application provides a dual-purpose ventilation system PCR laboratory, including:
an entry zone, the entry zone comprising:
a clean room;
the preparation room is communicated with the clean room through a door body;
the dressing room is communicated with the preparation room through a door body;
the special PCR walkway is communicated with the dressing room through a door body;
the reagent preparation area is communicated with the PCR special passage through a door body and is configured to prepare a storage reagent, split charging the reagent and prepare a main reaction mixed solution;
the sample preparation area is communicated with the PCR special passage through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into an amplification reaction tube and synthesize cDNA;
a gene amplification region, wherein the gene amplification region is communicated with the PCR dedicated walkway through a gate body and is configured to amplify DNA or cDNA;
a product analysis region, wherein the product analysis region is communicated with the PCR dedicated walkway through a gate body, and the product analysis region is configured to measure an amplified fragment;
an air exhaust device configured to exhaust air of the reagent preparation region, the sample preparation region, the gene amplification region, and the product analysis region to the outside;
an air supply device configured to simultaneously supply air into the reagent preparation zone, the sample preparation zone, the gene amplification zone, and the product analysis zone; the air supply device includes:
the air inlet end of the air generating part is not communicated with the air exhaust end of the air exhaust device;
an input port of the main air supply pipeline is communicated with an air outlet end of the air generating part, and the main air supply pipeline simultaneously extends into the reagent preparation area, the sample preparation area, the gene amplification area and the product analysis area;
the input end of the reagent preparation area air supply pipe is communicated with the air supply main pipeline, and the output end of the reagent preparation area air supply pipe extends into the reagent preparation area and is used for supplying air into the reagent preparation area;
the input end of the sample preparation area air supply pipe is communicated with the air supply main pipeline, and the output end of the sample preparation area air supply pipe extends into the sample preparation area and is used for supplying air into the sample preparation area;
the input end of the gene amplification area blast pipe is communicated with the blast main pipeline, and the output end of the gene amplification area blast pipe extends into the gene amplification area and is used for blasting air into the gene amplification area;
and the input end of the product analysis area air supply pipe is communicated with the air supply main pipeline, and the output end of the product analysis area air supply pipe extends into the product analysis area and is used for supplying air into the product analysis area.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:
the dual-purpose ventilation system PCR laboratory provided by the embodiment of the application is provided with an inlet area, a special PCR aisle, a reagent preparation area, a sample preparation area, a gene amplification area, a product analysis area, an exhaust device and an air supply device, wherein the air supply device comprises an air generating part, an air supply main pipeline, a reagent preparation area air supply pipe, a sample preparation area air supply pipe, a gene amplification area air supply pipe and a product analysis area air supply pipe, the air is generated by the air generating part, the air is respectively sent into the reagent preparation area through the reagent preparation area air supply pipe after entering the air supply main pipeline, is sent into the sample preparation area through the sample preparation area air supply pipe, is sent into the gene amplification area through the gene amplification area air supply pipe and is sent into the product analysis area through the product analysis area air supply pipe, the air is supplied by the same air generating part, and the air inlet end of the air generating part is not communicated with the exhaust end of the exhaust device, so that the air supply device and the exhaust, the circulating air is not formed, the possibility of air cross contamination can be effectively avoided, the health of medical research and inspection personnel is ensured under the condition of ensuring smooth ventilation of each area, the safety performance is effectively improved, test substances such as reagents and samples can be effectively prevented from being polluted, and the accuracy of a detection result is ensured.
And the air supply device and the air exhaust device are relatively independent, so that when a common PCR project without maintaining negative pressure is carried out, only the air exhaust device is started, the air supply device is closed, and energy is saved. When encountering Severe Acute Respiratory Syndrome (SARS), avian influenza infection, Middle East Respiratory Syndrome (MERS), novel coronavirus pneumonia (COVID-19) and other epidemic situations, the air exhaust device and the air supply device can be simultaneously opened, and circulating air is not formed, so that the air cross flow between all the areas is prevented, the safety performance of all the areas can be improved, the method is suitable for epidemic situation PCR projects, and dual purposes are realized.
Drawings
The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
In the drawings:
FIG. 1 is a plan view of a dual-purpose ventilated system PCR laboratory according to an embodiment of the present disclosure;
FIG. 2 is a schematic structural diagram of a dual-purpose ventilation system PCR laboratory provided in an embodiment of the present application;
FIG. 3 is a schematic structural diagram of the dual-purpose ventilation system PCR laboratory in FIG. 2 with the air exhausting device removed according to the embodiment of the present application;
FIG. 4 is a schematic structural diagram of the dual-purpose ventilation system PCR laboratory in FIG. 2 without an air blowing device according to an embodiment of the present disclosure;
FIG. 5 is a schematic diagram of a reagent preparation area provided in an embodiment of the present application;
FIG. 6 is a schematic structural diagram of a sample preparation area provided in an embodiment of the present application;
FIG. 7 is a schematic structural diagram of a gene amplification region provided in an example of the present application;
FIG. 8 is a schematic structural diagram of a product analysis region provided in an embodiment of the present application.
In the figure:
100. a dual-purpose ventilation system PCR laboratory; 10. entering a zone; 11. a clean room; 12. a preparation room; 13. a dressing room; 20. a PCR dedicated walkway; 70. an air exhaust device; 80. an air supply device; 81. a wind generating part; 82. a main air supply pipeline; 83. the reagent preparation area is provided with an air supply pipe; 831. a reagent preparation buffer room blast pipe; 832. an air supply pipe of the reagent preparation room; 84. a sample preparation area blast pipe; 841. a sample preparation buffer room blast pipe; 842. a sample preparation room blast pipe; 85. a blast pipe of the gene amplification area; 851. a gene amplification buffer room blast pipe; 852. an air supply pipe between gene amplification; 86. a product analysis zone blast pipe; 861. a product analysis buffer room blast pipe; 862. a product analysis room blast pipe; 30. a reagent preparation zone; 31. a reagent preparation buffer chamber; 32. a reagent preparation room; 71. the reagent preparation area is provided with an independent air exhaust device; 40. a sample preparation zone; 41. a sample preparation buffer room; 42. a sample preparation room; 72. the sample preparation area is provided with an independent air exhaust device; 50. a gene amplification region; 51. a gene amplification buffer; 52. during gene amplification; 73. an independent exhaust device in the gene amplification area; 60. a product analysis zone; 61. a product analysis buffer; 62. a product analysis room; 74. an independent exhaust device in the product analysis area; 711. the reagent preparation area is provided with an independent air exhaust main pipeline; 712. the reagent preparation area is provided with an independent exhaust fan; 713. the reagent preparation area air exhaust high-efficiency filter; 714. preparing an exhaust branch pipe of the buffer room by using the reagent; 715. an exhaust branch pipe of the reagent preparation room; 716. an air exhaust regulating valve of the reagent preparation buffer room; 717. an air exhaust regulating valve of the reagent preparation room; 718. a main air exhaust regulating valve in the reagent preparation area; 719. the reagent preparation area air exhaust check valve; 721. the sample preparation area is provided with an independent air exhaust main pipeline; 722. the sample preparation area is provided with an independent exhaust fan; 723. a sample preparation area air exhaust high-efficiency filter; 724. an exhaust branch pipe of the sample preparation buffer room; 725. an exhaust branch pipe of the sample preparation room; 726. an air exhaust regulating valve of the sample preparation buffer room; 727. an air exhaust adjusting valve of the sample preparation room; 728. a sample preparation area air exhaust main regulating valve; 729. a sample preparation area air exhaust check valve; 731. the gene amplification area is an independent air exhaust main pipeline; 732. a gene amplification region independent exhaust fan; 733. a high-efficiency exhaust filter in the gene amplification area; 734. an exhaust branch pipe of the gene amplification buffer room; 735. exhaust branch pipes between gene amplification; 736. an exhaust regulating valve of the gene amplification buffer room; 737. an air exhaust regulating valve between gene amplification; 738. a main air exhaust regulating valve in the gene amplification region; 739. an air exhaust check valve in the gene amplification area; 741. the product analysis area is provided with an independent air exhaust main pipeline; 742. an independent exhaust fan in the product analysis area; 743. a high-efficiency exhaust filter in the product analysis area; 744. product analysis buffer room exhaust branch pipe; 745. exhaust branch pipes of the product analysis room; 746. an exhaust regulating valve of the product analysis buffer room; 747. an exhaust regulating valve of the product analysis room; 748. a main exhaust regulating valve in the product analysis area; 749. product analysis district exhaust check valve.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
It is noted that, unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In the present invention, unless otherwise specified, the use of directional words such as "upper and lower" is generally in reference to the orientation shown in the drawings, or to the vertical, perpendicular or gravitational orientation; likewise, for ease of understanding and description, "left and right" are generally to the left and right as shown in the drawings; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself, but the above directional terms are not intended to limit the present invention.
In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In order to solve the technical problems that the health of medical research and inspection personnel is easily threatened and the detection result is inaccurate due to the adoption of a circulating ventilation mode among all the areas of a PCR laboratory in the prior art, the embodiment of the application provides a dual-purpose ventilation system PCR laboratory.
The present invention will be further explained with reference to the accompanying drawings.
As shown in fig. 1 to 8, the present embodiment provides a dual-purpose ventilated system PCR laboratory 100, which includes an entrance area 10, a PCR dedicated passage 20, a reagent preparation area 30, a sample preparation area 40, a gene amplification area 50, a product analysis area 60, an exhaust device 70, and an air supply device 80.
The access area 10 includes a clean room 11, a preparation room 12, and a dressing room 13. The preparation room 12 is communicated with the clean room 11 through a door body, and the dressing room 13 is communicated with the preparation room 12 through a door body. The clean room 11 is provided with an entrance door for medical research and inspection personnel to enter, and then sequentially passes through the clean room 11, the preparation room 12 and the dressing room 13 to enter the PCR passage 20.
The PCR dedicated passage 20 is communicated with the dressing room 13 through a door body, and the PCR dedicated passage 20 is a common corridor which enters the reagent preparation area 30, the sample preparation area 40, the gene amplification area 50 and the product analysis area 60. The air pressure of the PCR exclusive path 20 may be +10 PA.
The reagent preparation area 30 is communicated with the PCR dedicated passage through a door body, and is configured to prepare a stored reagent, split-package the reagent and prepare a main reaction mixed solution.
The sample preparation area 40 is communicated with the PCR dedicated walkway through a door body, and is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA.
The gene amplification region 50 is communicated with the PCR dedicated walkway through a gate body, and is configured to amplify DNA or cDNA.
The product analysis zone 60 is in communication with the PCR-specific walkway through the portal body, and the product analysis zone is configured to determine the amplified fragments.
The air exhaust device 70 is configured to exhaust air from the reagent preparation area, the sample preparation area, the gene amplification area, and the product analysis area to the outside.
The air blowing device 80 is configured to blow air into the reagent preparation region, the sample preparation region, the gene amplification region, and the product analysis region at the same time. The air blowing device 80 includes an air generating part 81, a main air blowing duct 82, a reagent preparation area air blowing duct 83, a sample preparation area air blowing duct 84, a gene amplification area air blowing duct 85, and a product analysis area air blowing duct 86.
The air intake end of the air generating part 81 is not communicated with the air exhaust end of the air exhaust device 70.
The input port of main air supply pipeline 82 is communicated with the air outlet end of air generating part 81, and the main air supply pipeline extends to the reagent preparation area, the sample preparation area, the gene amplification area and the product analysis area simultaneously, i.e. the main air supply pipeline penetrates through the reagent preparation area, the sample preparation area, the gene amplification area and the product analysis area simultaneously, i.e. the main air supply pipeline is positioned in the reagent preparation area, the sample preparation area, the gene amplification area and the product analysis area simultaneously.
The input end of the reagent preparation area air supply pipe 83 is communicated with the air supply main pipeline 82, and the output end thereof extends into the reagent preparation area for supplying air into the reagent preparation area.
The input end of the sample preparation area air supply pipe 84 is communicated with the air supply main pipeline 82, and the output end thereof extends into the sample preparation area for supplying air into the sample preparation area.
The input end of the gene amplification region air supply pipe 85 is communicated with the air supply main pipeline 82, and the output end thereof extends into the gene amplification region for supplying air into the gene amplification region.
The input end of the product analysis zone blast pipe 86 is communicated with the blast main pipe 82, and the output end thereof extends into the product analysis zone for blowing air into the product analysis zone.
The dual-purpose ventilation system PCR laboratory provided by the embodiment of the application is provided with an inlet area 10, a special PCR aisle 20, a reagent preparation area 30, a sample preparation area 40, a gene amplification area 50, a product analysis area 60, an exhaust device 70 and an air supply device 80, wherein the air supply device 80 comprises an air generating part 81, an air supply main pipeline 82, a reagent preparation area air supply pipeline 83, a sample preparation area air supply pipeline 84, a gene amplification area air supply pipeline 85 and a product analysis area air supply pipeline 86, air is generated through the air generating part 81, the air is respectively sent to the reagent preparation area 30 through the reagent preparation area air supply pipeline 83, sent to the sample preparation area 40 through the sample preparation area air supply pipeline 84, sent to the gene amplification area 50 through the gene amplification area air supply pipeline 85 and sent to the product analysis area 60 through the product analysis area air supply pipeline 86 after entering the air supply main pipeline 82, the air is supplied through the same air generating part 81, and the air inlet end of the air generating part 81 is not communicated with the exhaust end of the exhaust device 70, make air supply arrangement 80 and exhaust device 70 relatively independent, do not form the circulated air, can effectively reduce air cross contamination's possibility, under the smooth and easy circumstances of ventilation of guaranteeing each district, guarantee medical research and inspector's health, effectively improve the security performance to can effectively prevent test material such as reagent, sample from being polluted, guarantee testing result's accuracy. And the air supply device and the air exhaust device are relatively independent, so that when a common PCR project without maintaining negative pressure is carried out, only the air exhaust device is started, the air supply device is closed, and energy is saved. When encountering Severe Acute Respiratory Syndrome (SARS), avian influenza infection, Middle East Respiratory Syndrome (MERS), novel coronavirus pneumonia (COVID-19) and other epidemic situations, the air exhaust device and the air supply device can be simultaneously opened, and circulating air is not formed, so that the air cross flow between all the areas is prevented, the safety performance of all the areas can be improved, the method is suitable for epidemic situation PCR projects, and dual purposes are realized.
In this embodiment, the reagent preparing area 30 includes a reagent preparing buffer compartment 31 and a reagent preparing compartment 32.
The reagent preparation buffer room 31 is communicated with the special PCR passage 20 through a door body, and the reagent preparation buffer room 31 is a buffer space entering the reagent preparation room 32 from the special PCR passage 20. The air pressure of the reagent preparation buffer compartment 31 may be 0 PA.
The reagent preparation room 32 is communicated with the reagent preparation buffer room 31 through a door body, and the reagent preparation room 32 is configured to prepare a stored reagent, split the reagent and prepare a main reaction mixed solution. The reagents and materials for specimen preparation are directly transported to the reagent preparing room 32, and the reagent raw materials are stored in the reagent preparing room 32. The reagent preparation room 32 can be equipped with a 2-8 ℃ refrigerator and a-80 ℃ refrigerator. The area of the room may be 15m2~20m2. The pressure gradient of the reagent preparation chamber 32 may be in a relatively positive pressure state (may be +10PA) to prevent the outside air containing nucleic acid aerosol from entering and causing pollution. A reagent preparation room air conditioner is provided in the reagent preparation room 32, and is configured to adjust the temperature and humidity inside the reagent preparation room.
Wherein, the reagent preparing area blast pipe 83 includes a reagent preparing buffer room blast pipe 831 and a reagent preparing room blast pipe 832.
The input end of the reagent preparation buffer room blast pipe 831 is communicated with the blast main pipe 82, and the output end thereof extends into the reagent preparation buffer room 31 for blowing air into the reagent preparation buffer room 31.
The input end of the reagent preparation room blast pipe 832 is communicated with the blast main pipe 82, and the output end thereof extends into the reagent preparation room 32 for blowing air into the reagent preparation room 32.
With the above arrangement, air is simultaneously supplied into the reagent preparing buffer room 31 and the reagent preparing room 32.
In this embodiment, the sample preparation area 40 includes a sample preparation buffer compartment 41 and a sample preparation compartment 42.
The sample preparation buffer room 41 is communicated with the PCR special passage 20 through a door body. The sample preparation buffer room 41 is a buffer space that enters the sample preparation room 42 from the PCR-dedicated lane 20. The air pressure of the sample preparation buffer 41 may be 0 PA.
The sample preparation room 42 is communicated with the sample preparation buffer room 41 through a door body, and the sample preparation room 42 is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA. The sample preparation room 42 may be equipped with a 2-8 ℃ refrigerator and a-20 ℃ refrigerator. The sample preparation room 42 is equipped with a biosafety cabinet for performing operations for extracting nucleic acids. To avoid repeated cycling of the extracted nucleic acids within the cabinet, resulting in cross-contamination between samples, and false positive results, the sample preparation booth 42 must be equipped with a biosafety cabinet of type B2. The vertical air flow of the working area of the biological safety cabinet comes from a laboratory, and the exhausted air is directly exhausted to the outside after being filtered by the high-efficiency filter and is not allowed to return to the safety cabinet and the laboratory. The area of the sample preparation compartment 42 may be 25m2~30m2In the meantime. The pressure gradient in the sample preparation bay 42 is desirably positive with respect to the adjacent area (which may be-10 PA) to avoid aerosol contamination from the adjacent area into the local area. A sample preparation room air conditioner is disposed in the sample preparation room 42 and configured to adjust the temperature and humidity inside the sample preparation room.
The sample preparation area blast pipe 84 includes a sample preparation buffer compartment blast pipe 841 and a sample preparation compartment blast pipe 842.
The input end of the sample preparation buffer room blast pipe 841 is communicated with the blast main pipeline 82, and the output end of the sample preparation buffer room blast pipe 841 extends into the sample preparation buffer room and is used for blasting air into the sample preparation buffer room.
The input end of the sample preparation room blast pipe 842 is communicated with the blast main pipeline 82, and the output end thereof extends into the sample preparation room and is used for blowing air into the sample preparation room.
In this embodiment, the gene amplification region 50 includes a gene amplification buffer room 51 and a gene amplification room 52.
The gene amplification buffer room 51 is communicated with the PCR special passage 20 through a door body. The gene amplification buffer room 51 is a buffer space which enters the gene amplification room 52 from the PCR dedicated path 20. The gas pressure in the gene amplification buffer 51 may be 0 PA.
The gene amplification room 52 communicates with the gene amplification buffer room 51 through a gate body, and the gene amplification room 52 is configured to amplify DNA or cDNA. In addition, addition of the prepared DNA template and synthesized cDNA (from the sample preparation room 42), preparation of the main reaction mixture (from the reagent preparation room 32) into a reaction mixture, and the like may be performed in the gene amplification room 52. In nested PCR assays, the reaction tube must usually be opened after the first round of amplification, so nested amplification has a high risk of contamination, and the second sample addition must be performed in the gene amplification chamber 52. The gene amplification room 52 is equipped with the core instrument of the PCR laboratory: as the PCR instrument, for example, two types of PCR instruments, ABI7500 and ABI9700, can be used. The area of the gene amplification chamber 52 can be controlled to be 15m2~20m2. The pressure gradient of the gene amplification chamber 52 is required to be negative pressure with respect to the adjacent region (the gene amplification chamber 52 may be-15 PA) to avoid the escape of aerosol from the region. To avoid contamination by aerosols, unnecessary movement within the region should be minimized. The gene amplification room 52 is provided with a gene amplification room air conditioner configured to regulate the temperature and humidity inside the gene amplification room 52.
The air supply duct 85 for the gene amplification region includes an air supply duct 851 for the buffer for gene amplification and an air supply duct 852 for the buffer for gene amplification.
The input end of the air supply pipe 851 of the gene amplification buffer chamber is communicated with the air supply main pipe 82, and the output end thereof extends into the gene amplification buffer chamber for supplying air into the gene amplification buffer chamber.
The input end of the air supply pipe 852 of the gene amplification chamber is communicated with the air supply main pipe 82, and the output end thereof extends into the gene amplification chamber and is used for supplying air to the gene amplification chamber.
In this embodiment, product analysis zone 60 includes a product analysis buffer compartment 61 and a product analysis compartment 62.
The product analysis buffer room 61 is communicated with the PCR special passage 20 through a door body. The product analysis buffer room 61 is a buffer channel from the PCR dedicated walkway 20 into the product analysis room 62. The pressure in product analysis buffer 61 may be 0 PA.
The product analysis room 62 communicates with the product analysis buffer room 61 through a door, and the product analysis room 62 is configured to measure the amplified fragment.The area of the product analysis chamber 62 can be controlled to be 15m2~20m2. The product analysis chamber 62 is the most important source of contamination of the amplification product, and therefore the pressure gradient in this region is required to be negative with respect to the adjacent region (the product analysis chamber 62 may be-20 PA) to prevent diffusion of the amplification product from this region to other regions. A product analysis room air conditioner is provided in the product analysis room 62, and is configured to adjust the temperature and humidity inside the product analysis room 62.
Among them, the product analysis zone blast duct 86 includes a product analysis buffer room blast duct 861 and a product analysis room blast duct 862.
The input end of the product analysis buffer air supply pipe 861 is communicated with the air supply main pipeline 82, and the output end thereof extends into the product analysis buffer room and is used for supplying air into the product analysis buffer room.
The input end of the product analysis room blast pipe 862 is communicated with the blast main pipe 82, and the output end thereof extends into the product analysis room for blowing air into the product analysis room.
In this embodiment, the wind generating portion 81 may be a purification air conditioning unit or a fan.
In this embodiment, the main air supply conduit 82 may be disposed along the length direction of the PCR laboratory and located above the reagent preparation area, the sample preparation area, the gene amplification area, and the product analysis area.
Reagent preparation buffer room blast pipe 831 can be located at the top of the reagent preparation buffer room, reagent preparation room blast pipe 832 can be located at the top of the reagent preparation room, sample preparation buffer room blast pipe 841 can be located at the top of the sample preparation buffer room, sample preparation room blast pipe 842 can be located at the top of the sample preparation room, gene amplification buffer room blast pipe 851 can be located at the top of the gene amplification buffer room, gene amplification room blast pipe 852 can be located at the top of the gene amplification room, product analysis buffer room blast pipe 861 can be located at the top of the product analysis buffer room, and product analysis room blast pipe 862 can be located at the top of the product analysis room. The wind generating part 81 may be provided outside the PCR laboratory or in any region of the PCR laboratory.
In this embodiment, a reagent preparation area filter is disposed at one end of the reagent preparation area air supply pipe 83 extending into the reagent preparation area, and the reagent preparation area filter may be at least one of a low, medium and high efficiency filter. Specifically, a reagent preparation area filter is arranged at the air outlet end of the reagent preparation buffer room air supply pipe 831, and a reagent preparation area filter is arranged at the air outlet end of the reagent preparation room air supply pipe 832. So as to ensure the safety of air supply.
In this embodiment, a sample preparation area filter is disposed at one end of the sample preparation area air supply pipe 84 extending into the sample preparation area, and the sample preparation area filter may be at least one of a low, medium, and high efficiency filter. Specifically, a sample preparation area filter is arranged at the air outlet end of the sample preparation buffer room blast pipe 841, and a sample preparation area filter is arranged at the air outlet end of the sample preparation room blast pipe 842. So as to ensure the safety of air supply.
In this embodiment, a gene amplification region filter is provided at one end of the gene amplification region air supply pipe 85 extending into the gene amplification region, and the gene amplification region filter may be at least one of a low, medium, and high efficiency filter. Specifically, the air outlet end of the air supply pipe 851 in the gene amplification buffer room is provided with a gene amplification region filter, and the air outlet end of the air supply pipe 852 in the gene amplification room is provided with a gene amplification region filter. So as to ensure the safety of air supply.
In this embodiment, a product analysis area filter is disposed at one end of the product analysis area air supply pipe 86 extending into the product analysis area, and the product analysis area filter may be at least one of a low, medium, and high efficiency filter. Specifically, a product analysis area filter is arranged at the air outlet end of the product analysis buffer air supply pipe 861, and a product analysis area filter is arranged at the air outlet end of the product analysis buffer air supply pipe 862. So as to ensure the safety of air supply.
In this embodiment, the reagent preparation buffer room air supply adjusting valve is disposed on the reagent preparation buffer room air supply pipe 831, so as to adjust the flow rate of air supplied to the reagent preparation buffer room. The air supply pipe 832 of the reagent preparation room is provided with an air supply adjusting valve of the reagent preparation room so as to adjust the flow of air supplied to the reagent preparation room.
In this embodiment, the sample preparation buffer room air supply pipe 841 is provided with a sample preparation buffer room air supply regulating valve so as to regulate the flow of air supplied to the sample preparation buffer room. The sample preparation room air supply pipe 842 is provided with a sample preparation room air supply adjusting valve so as to adjust the flow of air supplied to the sample preparation room.
In this embodiment, the blower pipe 851 of the gene amplification buffer room is provided with a blower adjusting valve of the gene amplification buffer room so as to adjust the flow rate of the air supplied into the gene amplification buffer room. The air supply duct 852 of the gene amplification chamber is provided with an air supply regulating valve of the gene amplification chamber so as to regulate the flow rate of air supplied into the gene amplification chamber.
In this embodiment, the product analysis buffer room air supply regulating valve is provided on the product analysis buffer room air supply pipe 861, so as to regulate the flow rate of air supply into the product analysis buffer room. The product analysis room blast pipe 862 is provided with a product analysis room blast adjusting valve so as to adjust the flow rate of the blast into the product analysis room.
Preferably, a check valve is respectively arranged on the reagent preparation buffer room blast pipe 831, the reagent preparation room blast pipe 832, the sample preparation buffer room blast pipe 841, the sample preparation room blast pipe 842, the gene amplification buffer room blast pipe 851, the gene amplification room blast pipe 852, the product analysis buffer room blast pipe 861 and the product analysis room blast pipe 862 to prevent air cross contamination caused by air streaming between each room due to positive pressure reflux between each room to other rooms.
In this embodiment, the exhaust device 70 includes a reagent preparation area independent exhaust device 71, a sample preparation area independent exhaust device 72, a gene amplification area independent exhaust device 73, and a product analysis area independent exhaust device 74.
The reagent preparing section independent air exhausting device 71 is configured to exhaust air in the reagent preparing section to the outside of the reagent preparing section.
The sample preparation section independent air-discharge device 72 is configured to discharge air in the sample preparation section to the outside of the sample preparation section.
The gene amplification region independent air exhausting means 73 is configured to exhaust the air in the gene amplification region to the outside of the gene amplification region.
Product analysis zone independent exhaust 74 is configured to exhaust air in the product analysis zone to the exterior of the product analysis zone.
Wherein the reagent preparation area independent air exhaust device 71, the sample preparation area independent air exhaust device 72, the gene amplification area independent air exhaust device 73 and the product analysis area independent air exhaust device 74 are independent and not communicated with each other.
By adopting the arrangement, independent air exhaust of each area is realized, air series flow between the areas is further prevented, and the safety performance is improved.
In the present embodiment, the reagent preparing section independent air exhausting device 71 is configured to simultaneously exhaust the air in the reagent preparing compartment 32 and the air in the reagent preparing buffer compartment 31 to the outside.
The independent exhaust device 71 for the reagent preparation area comprises a main independent exhaust pipeline 711 for the reagent preparation area, an independent exhaust fan 712 for the reagent preparation area, an efficient exhaust filter 713 for the reagent preparation area, an exhaust branch pipe 714 for the reagent preparation buffer room, and an exhaust branch pipe 715 for the reagent preparation room.
The reagent preparation area independent air exhaust main pipe 711 is arranged in the reagent preparation room 32, and one end of the reagent preparation area independent air exhaust main pipe 711 is communicated with the outside, namely an air exhaust end.
The reagent preparing area independent exhaust blower 712 is disposed on the reagent preparing area independent exhaust main duct 711, and is configured to provide exhaust power.
The reagent preparing zone exhaust air high-efficiency filter 713 is disposed on the reagent preparing zone independent exhaust air main pipe 711, and is configured to filter the exhausted air and prevent harmful substances and germs from being exhausted. The reagent preparing area exhaust high-efficiency filter 713 may be disposed on the exhaust side of the reagent preparing area independent exhaust fan 712 on the reagent preparing area independent exhaust main duct 711.
One end of the reagent preparation buffering room exhaust branch pipe 714 is communicated with the reagent preparation area independent exhaust main pipe 711, and the other end of the reagent preparation buffering room exhaust branch pipe 714 is communicated with the reagent preparation buffering room 31.
One end of the exhaust branch pipe 715 of the reagent preparation room is communicated with the independent exhaust main pipe 711 of the reagent preparation area, and the other end of the exhaust branch pipe 715 of the reagent preparation room is communicated with the reagent preparation room 32.
Through the operation of the reagent preparation area independent exhaust fan 712, the air in the reagent preparation buffer room 31 enters the reagent preparation area independent exhaust main pipe 711 through the reagent preparation buffer room exhaust branch pipe 714, the air in the reagent preparation room 32 enters the reagent preparation area independent exhaust main pipe 711 through the reagent preparation room exhaust branch pipe 715, and the reagent preparation area independent exhaust fan 712 exhausts the air to the reagent preparation area exhaust high-efficiency filter 713, and the air is filtered by the reagent preparation area exhaust high-efficiency filter 713 and then exhausted to the outside. The simultaneous discharge of the air in the reagent preparing compartment 32 and the air in the reagent preparing buffer compartment 31 is achieved.
Preferably, the reagent preparation area independent exhaust device 71 further comprises a reagent preparation buffer room exhaust adjusting valve 716 and a reagent preparation room exhaust adjusting valve 717.
The air exhaust regulating valve 716 of the reagent preparation buffer room is arranged on the air exhaust branch pipe 714 of the reagent preparation buffer room. The exhaust regulating valve 717 of the reagent preparing room is provided on the exhaust branch pipe 715 of the reagent preparing room. By adopting the arrangement, the air exhaust speed and the air exhaust amount of the reagent preparation buffer room 31 and the air exhaust speed and the air exhaust amount of the reagent preparation room 32 can be conveniently and respectively adjusted, so that the air pressure can be conveniently adjusted.
Preferably, the reagent preparation area independent exhaust device 71 further comprises a reagent preparation area main exhaust adjusting valve 718, and the reagent preparation area main exhaust adjusting valve 718 is disposed on the reagent preparation area independent exhaust main pipe 711 and is located on the air intake side of the reagent preparation area independent exhaust fan 712. With the arrangement, the total exhaust flow of the main independent exhaust duct 711 in the reagent preparation area can be conveniently adjusted. Be convenient for with the cooperation of air discharge regulating valve 716 and the air discharge regulating valve 717 between reagent preparation buffering, carry out whole accurate regulation.
Preferably, the reagent preparation area independent exhaust device 71 further includes a reagent preparation area exhaust check valve 719, and the reagent preparation area exhaust check valve 719 is disposed on the reagent preparation area independent exhaust main pipe 711 and located on an air outlet side of the reagent preparation area independent exhaust fan 712. With the above arrangement, the backflow of air into the reagent preparing compartment 32 and the reagent preparing buffer compartment 31 can be prevented.
Preferably, the end of the reagent preparation buffer compartment exhaust branch pipe 714 communicated with the reagent preparation buffer compartment 31 is located at the entrance of the reagent preparation buffer compartment 31 far away from the reagent preparation buffer compartment 31 and at the entrance far away from the reagent preparation compartment 32.
Preferably, the end of the reagent preparation room exhaust manifold 715 in communication with the reagent preparation room 32 is located at an entrance door of the reagent preparation room 32 remote from the reagent preparation room 32. By adopting the arrangement, the airflow flows from the low pollution risk area to the high pollution risk area.
In the present embodiment, the sample-preparation-area independent air-discharge device 72 is configured to discharge the air in the sample preparation compartment 42 and the air in the sample preparation buffer compartment 41 to the outside at the same time.
In this embodiment, the sample preparation area independent air exhaust device 72 includes a sample preparation area independent air exhaust main pipe 721, a sample preparation area independent air exhaust blower 722, a sample preparation area air exhaust high-efficiency filter 723, a sample preparation buffer room air exhaust branch pipe 724, and a sample preparation room air exhaust branch pipe 725.
The main independent air exhaust pipe 721 of the sample preparation area is disposed in the sample preparation room 42, and one end of the main independent air exhaust pipe 721 of the sample preparation area is communicated with the outside.
The sample preparation area independent exhaust fan 722 is disposed on the sample preparation area independent exhaust main pipe 721 and configured to provide an exhaust power.
The sample preparation area air discharge high-efficiency filter 723 is disposed on the sample preparation area independent air discharge main pipe 721 and configured to filter discharged air and prevent discharge of harmful substances and germs. The sample preparation area exhaust high-efficiency filter 723 may be disposed on the exhaust side of the sample preparation area independent exhaust blower 722 on the sample preparation area independent exhaust main pipe 721.
One end of the sample preparation buffer room exhaust branch pipe 724 is communicated with the sample preparation area independent exhaust main pipe 721, and the other end of the sample preparation buffer room exhaust branch pipe 724 is communicated with the sample preparation buffer room 41.
One end of the exhaust branch pipe 725 of the sample preparation room is communicated with the main independent exhaust pipe 721 of the sample preparation area, and the other end of the exhaust branch pipe 725 of the sample preparation room is communicated with the sample preparation room 42.
Through the work of the sample preparation area independent exhaust blower 722, the air in the sample preparation buffer room is exhausted to the sample preparation area independent exhaust main pipeline 721 through the sample preparation buffer room exhaust branch pipe 724, the air in the sample preparation room is exhausted to the sample preparation area independent exhaust main pipeline 721 through the sample preparation room exhaust branch pipe 725, the sample preparation area independent exhaust blower 722 exhausts the air to the sample preparation area exhaust efficient filter 723, and the air is exhausted to the outside after passing through the sample preparation area exhaust efficient filter 723.
Preferably, the sample preparation area independent air exhaust device 72 further includes a sample preparation buffer compartment air exhaust regulating valve 726 and a sample preparation compartment air exhaust regulating valve 727.
The sample preparation buffer room exhaust regulating valve 726 is arranged on the sample preparation buffer room exhaust branch pipe 724; the sample preparation room exhaust regulating valve 727 is arranged on the sample preparation room exhaust branch pipe 725.
Preferably, the sample preparation area independent air exhaust device 72 further includes a sample preparation area main air exhaust adjusting valve 728, and the sample preparation area main air exhaust adjusting valve 728 is disposed on the sample preparation area independent air exhaust main pipe 721 and located on the air intake side of the sample preparation area independent air exhaust fan 722.
Preferably, the sample preparation area independent air exhaust device 72 further includes a sample preparation area air exhaust check valve 729, and the sample preparation area air exhaust check valve 729 is disposed on the sample preparation area independent air exhaust main pipe 721 and is located on the air outlet side of the sample preparation area independent air exhaust fan 722.
Preferably, the end of the sample preparation buffer compartment exhaust branch pipe 724 communicated with the sample preparation buffer compartment 41 is located at the entrance of the sample preparation buffer compartment 41 far away from the sample preparation buffer compartment 41 and at the entrance far away from the sample preparation compartment 42.
Preferably, the end of the sample preparation room exhaust manifold 725 that communicates with the sample preparation room 42 is located at the entrance of the sample preparation room 42 that is remote from the sample preparation room 42. By adopting the arrangement, the airflow flows from the low pollution risk area to the high pollution risk area.
In this embodiment, the gene amplification region independent air exhausting device 73 is configured to simultaneously exhaust the air in the gene amplification chamber 52 and the air in the gene amplification buffer chamber 51 to the outside.
Preferably, the gene amplification region independent exhaust device 73 includes a gene amplification region independent exhaust main pipe 731, a gene amplification region independent exhaust fan 732, a gene amplification region exhaust high-efficiency filter 733, a gene amplification buffer chamber exhaust branch pipe 734, and a gene amplification chamber exhaust branch pipe 735.
The main independent exhaust pipe 731 of the gene amplification region is arranged in the gene amplification room 52, and one end of the main independent exhaust pipe 731 of the gene amplification region is communicated with the outside.
The gene amplification region independent exhaust fan 732 is disposed on the gene amplification region independent exhaust main pipe 731, and is configured to provide exhaust power.
The gene amplification region exhaust high-efficiency filter 733 is disposed on the gene amplification region independent exhaust main pipe 731, and is configured to filter the exhaust air.
One end of the exhaust branch pipe 734 of the gene amplification buffer room is communicated with the main independent exhaust pipe 731 of the gene amplification region, and the other end of the exhaust branch pipe 734 of the gene amplification buffer room is communicated with the gene amplification buffer room 51.
One end of the exhaust branch pipe 735 between gene amplifications is communicated with the main independent exhaust pipe 731 of the gene amplification region, and the other end of the exhaust branch pipe 735 between gene amplifications is communicated with the gene amplification chamber 52.
The independent exhaust fan 732 of the gene amplification region works to provide exhaust power, so that the air in the gene amplification buffer room enters the main independent exhaust pipe 731 of the gene amplification region through the gene amplification buffer room, the air in the gene amplification room enters the main independent exhaust pipe 731 of the gene amplification region through the exhaust branch pipe 735 of the gene amplification room, and is filtered by the high-efficiency exhaust filter 733 of the gene amplification region and then is exhausted outdoors.
Preferably, the gene amplification region independent exhaust device 73 further comprises a gene amplification buffer room exhaust adjusting valve 736 and a gene amplification room exhaust adjusting valve 737.
The gene amplification buffer room exhaust regulating valve 736 is arranged on the gene amplification buffer room exhaust branch pipe 734; the exhaust adjusting valve 737 between gene amplifications is provided to the exhaust branch pipe 735 between gene amplifications.
Preferably, the gene amplification region independent exhaust device 73 further comprises a gene amplification region main exhaust air regulating valve 738, and the gene amplification region main exhaust air regulating valve 738 is arranged on the gene amplification region independent exhaust main pipe 731 and is positioned on the air inlet side of the gene amplification region independent exhaust fan 732.
Preferably, the gene amplification region independent exhaust device 73 further comprises a gene amplification region exhaust check valve 739, and the gene amplification region exhaust check valve 739 is disposed on the gene amplification region independent exhaust main pipe 731 and is located on the air outlet side of the gene amplification region independent exhaust fan 732.
Preferably, the end of the exhaust branch 734 of the gene amplification buffer room communicating with the gene amplification buffer room 51 is located at the entrance of the gene amplification buffer room 51 far from the gene amplification buffer room 51 and at the entrance far from the gene amplification buffer room 52.
Preferably, the end of the gene amplification chamber exhaust branch pipe 735 that communicates with the gene amplification chamber 52 is located at the door entrance of the gene amplification chamber 52 that is far from the gene amplification chamber 52.
In the present embodiment, product analysis zone independent air discharge device 74 is configured to discharge air in product analysis compartment 62 and air in product analysis buffer compartment 61 to the outside simultaneously.
In this embodiment, the independent exhaust device 74 of the product analysis area includes a main independent exhaust pipe 741 of the product analysis area, an independent exhaust fan 742 of the product analysis area, an efficient exhaust filter 743 of the product analysis area, a branch exhaust pipe 744 of the product analysis buffer room, and a branch exhaust pipe 745 of the product analysis room.
The main independent air exhaust duct 741 in the product analysis area is disposed in the product analysis room 62, and one end of the main independent air exhaust duct 741 in the product analysis area is communicated with the outside.
The product analysis area independent exhaust fan 742 is disposed on the product analysis area independent exhaust main pipe 741, and is configured to provide exhaust power.
The product analysis area air exhaust high-efficiency filter 743 is arranged on the product analysis area independent air exhaust main pipe 741 and is configured to filter the exhausted air.
One end of the exhaust branch pipe 744 of the product analysis buffer room is communicated with the independent exhaust main pipe 741 of the product analysis area, and the other end of the exhaust branch pipe 744 of the product analysis buffer room is communicated with the product analysis buffer room 61.
One end of the exhaust branch pipe 745 of the product analysis room is communicated with the independent exhaust main pipe 741 of the product analysis area, and the other end of the exhaust branch pipe 745 of the product analysis room is communicated with the product analysis room 62.
The independent exhaust fan 742 in the product analysis area works to provide exhaust power, so that air in the product analysis buffer room is exhausted to the independent exhaust main pipeline 741 in the product analysis area through the product analysis room, the air in the product analysis room is exhausted to the independent exhaust main pipeline 741 in the product analysis area through the exhaust branch pipe 745 in the product analysis room, and the air is filtered by the exhaust high-efficiency filter 743 in the product analysis area and then is exhausted to the outside.
Preferably, product analysis area independent exhaust 74 also includes product analysis buffer exhaust damper 746 and product analysis room exhaust damper 747.
The product analysis buffer room exhaust regulating valve 746 is arranged on the product analysis buffer room exhaust branch tube 744; the product analysis room exhaust adjusting valve 747 is provided on the product analysis room exhaust branch pipe 745.
Preferably, the product analysis area independent exhaust device 74 further includes a product analysis area main exhaust adjusting valve 748 disposed on the product analysis area independent exhaust main pipe 741 and located on an air intake side of the product analysis area independent exhaust fan 742.
Preferably, the product analysis area independent exhaust device 74 further includes a product analysis area exhaust check valve 749, which is disposed on the product analysis area independent exhaust main pipe 741 and is located on the air outlet side of the product analysis area independent exhaust fan 742.
Preferably, the end of product analysis buffer exhaust branch 744 that communicates with product analysis buffer compartment 61 is located at an entrance of product analysis buffer compartment 61 that is distal from product analysis buffer compartment 61 and at an entrance that is distal from product analysis buffer compartment 62.
Preferably, the end of product analysis bay exhaust duct 745 that communicates with product analysis bay 62 is located at an entrance to product analysis bay 62 that is remote from product analysis bay 62.
Preferably, the above-mentioned regulating valves may be electric regulating valves or manual regulating valves.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the creative work should be covered within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.

Claims (9)

1. A dual-purpose ventilated system PCR laboratory, comprising:
an entry zone, the entry zone comprising:
a clean room;
the preparation room is communicated with the clean room through a door body; and
the dressing room is communicated with the preparation room through a door body;
the special PCR walkway is communicated with the dressing room through a door body;
the reagent preparation area is communicated with the PCR special passage through a door body and is configured to prepare a storage reagent, split charging the reagent and prepare a main reaction mixed solution;
the sample preparation area is communicated with the PCR special passage through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into an amplification reaction tube and synthesize cDNA;
a gene amplification region, wherein the gene amplification region is communicated with the PCR dedicated walkway through a gate body and is configured to amplify DNA or cDNA;
a product analysis region, wherein the product analysis region is communicated with the PCR dedicated walkway through a gate body, and the product analysis region is configured to measure an amplified fragment;
an air exhaust device configured to exhaust air of the reagent preparation region, the sample preparation region, the gene amplification region, and the product analysis region to the outside; and
an air supply device configured to simultaneously supply air into the reagent preparation zone, the sample preparation zone, the gene amplification zone, and the product analysis zone; the air supply device includes:
the air inlet end of the air generating part is not communicated with the air exhaust end of the air exhaust device;
an input port of the main air supply pipeline is communicated with an air outlet end of the air generating part, and the main air supply pipeline simultaneously extends into the reagent preparation area, the sample preparation area, the gene amplification area and the product analysis area;
the input end of the reagent preparation area air supply pipe is communicated with the air supply main pipeline, and the output end of the reagent preparation area air supply pipe extends into the reagent preparation area and is used for supplying air into the reagent preparation area;
the input end of the sample preparation area air supply pipe is communicated with the air supply main pipeline, and the output end of the sample preparation area air supply pipe extends into the sample preparation area and is used for supplying air into the sample preparation area;
the input end of the gene amplification area blast pipe is communicated with the blast main pipeline, and the output end of the gene amplification area blast pipe extends into the gene amplification area and is used for blasting air into the gene amplification area; and
and the input end of the product analysis area air supply pipe is communicated with the air supply main pipeline, and the output end of the product analysis area air supply pipe extends into the product analysis area and is used for supplying air into the product analysis area.
2. The dual-purpose ventilated system PCR laboratory according to claim 1, wherein said reagent preparation section comprises:
the reagent preparation buffer room is communicated with the PCR special passage through a door body; and
the reagent preparation room is communicated with the reagent preparation buffer room through a door body and is configured to prepare a stored reagent, split-package the reagent and prepare a main reaction mixed solution;
wherein, reagent preparation district blast pipe includes:
the input end of the reagent preparation buffer room air supply pipe is communicated with the air supply main pipeline, and the output end of the reagent preparation buffer room air supply pipe extends into the reagent preparation buffer room and is used for supplying air into the reagent preparation buffer room;
and the input end of the air supply pipe of the reagent preparation room is communicated with the air supply main pipeline, and the output end of the air supply pipe extends into the reagent preparation room and is used for supplying air into the reagent preparation room.
3. The dual-purpose ventilated system PCR laboratory according to claim 1, wherein said sample preparation zone comprises:
the sample preparation buffer room is communicated with the PCR special passage through a door body; and
the sample preparation room is communicated with the sample preparation buffer room through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into the amplification reaction tube and synthesize cDNA;
wherein, sample preparation zone blast pipe includes:
the input end of the sample preparation buffer room air supply pipe is communicated with the air supply main pipeline, and the output end of the sample preparation buffer room air supply pipe extends into the sample preparation buffer room and is used for supplying air into the sample preparation buffer room;
and the input end of the sample preparation room air supply pipe is communicated with the air supply main pipeline, and the output end of the sample preparation room air supply pipe extends into the sample preparation room and is used for supplying air into the sample preparation room.
4. The dual-purpose ventilated system PCR laboratory according to claim 1, wherein said gene amplification region comprises:
the gene amplification buffer room is communicated with the PCR special walkway through a door body; and
a gene amplification chamber, wherein the gene amplification chamber is communicated with the gene amplification buffer chamber through a door body, and the gene amplification chamber is configured to amplify DNA or cDNA;
wherein the air supply pipe of the gene amplification area comprises:
the input end of the gene amplification buffer room blast pipe is communicated with the blast main pipeline, and the output end of the gene amplification buffer room blast pipe extends into the gene amplification buffer room and is used for blasting air into the gene amplification buffer room;
and the input end of the air supply pipe of the gene amplification room is communicated with the air supply main pipeline, and the output end of the air supply pipe of the gene amplification room extends into the gene amplification room and is used for supplying air into the gene amplification room.
5. The dual-purpose ventilated system PCR laboratory according to claim 1, wherein said product analysis zone comprises:
a product analysis buffer room communicated with the PCR special passage through a door body; and
a product analysis room, wherein the product analysis room is communicated with the product analysis buffer room through a door body, and the product analysis room is configured to measure the amplified fragments;
wherein the product analysis zone air supply duct comprises:
the input end of the product analysis buffer room blast pipe is communicated with the blast main pipeline, and the output end of the product analysis buffer room blast pipe extends into the product analysis buffer room and is used for blasting air into the product analysis buffer room;
and the input end of the product analysis room air supply pipe is communicated with the air supply main pipeline, and the output end of the product analysis room air supply pipe extends into the product analysis room and is used for supplying air to the product analysis room.
6. The dual-purpose ventilation system PCR laboratory according to claim 1, wherein the air exhausting device comprises:
a reagent preparation zone independent exhaust device configured to exhaust air in the reagent preparation zone to an exterior of the reagent preparation zone;
a sample preparation zone independent exhaust device configured to exhaust air in the sample preparation zone to an exterior of the sample preparation zone;
a gene amplification region independent air discharge device configured to discharge air in the gene amplification region to the outside of the gene amplification region; and
a product analysis zone independent exhaust configured to exhaust air in the product analysis zone to an exterior of the product analysis zone;
wherein the reagent preparation area independent air exhaust device, the sample preparation area independent air exhaust device, the gene amplification area independent air exhaust device and the product analysis area independent air exhaust device are independent and not communicated with each other.
7. The dual-purpose ventilated system PCR laboratory of claim 1, wherein:
the wind generating part is a purifying air conditioning unit or a fan.
8. The dual-purpose ventilated system PCR laboratory of claim 1, wherein:
a reagent preparation area filter is arranged at one end of the reagent preparation area air supply pipe extending into the reagent preparation area;
a sample preparation area filter is arranged at one end of the sample preparation area air supply pipe extending into the sample preparation area;
one end of the air supply pipe of the gene amplification region, which extends into the gene amplification region, is provided with a gene amplification region filter;
and a product analysis area filter is arranged at one end of the product analysis area blast pipe extending into the product analysis area.
9. The dual-purpose ventilated system PCR laboratory of claim 2, wherein:
the reagent preparation buffer room air supply pipe is provided with a reagent preparation buffer room air supply regulating valve;
and an air supply adjusting valve of the reagent preparation room is arranged on the air supply pipe of the reagent preparation room.
CN202021425317.7U 2020-07-20 2020-07-20 Dual-purpose ventilation system PCR laboratory Active CN212538176U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021425317.7U CN212538176U (en) 2020-07-20 2020-07-20 Dual-purpose ventilation system PCR laboratory

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021425317.7U CN212538176U (en) 2020-07-20 2020-07-20 Dual-purpose ventilation system PCR laboratory

Publications (1)

Publication Number Publication Date
CN212538176U true CN212538176U (en) 2021-02-12

Family

ID=74517459

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021425317.7U Active CN212538176U (en) 2020-07-20 2020-07-20 Dual-purpose ventilation system PCR laboratory

Country Status (1)

Country Link
CN (1) CN212538176U (en)

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