CN212773666U - PCR laboratory with dual-purpose type dressing room - Google Patents
PCR laboratory with dual-purpose type dressing room Download PDFInfo
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- CN212773666U CN212773666U CN202021435877.0U CN202021435877U CN212773666U CN 212773666 U CN212773666 U CN 212773666U CN 202021435877 U CN202021435877 U CN 202021435877U CN 212773666 U CN212773666 U CN 212773666U
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Abstract
The utility model discloses a PCR laboratory with dual-purpose type dressing room belongs to PCR laboratory field, include: an entry zone, the entry zone comprising: a clean room; the preparation room is communicated with the clean room through a door body; a dressing room, comprising: the first dressing room is communicated with the preparation room through a first door body; the air shower room is communicated with the first dressing room through a second door body; the second dressing room is communicated with the air shower room through a third door body; the PCR special walkway is communicated with the first dressing room through a fourth door body and is communicated with the second dressing room through a fifth door body; a reagent preparation zone; a sample preparation zone; a gene amplification region; a product analysis zone; wherein, the dressing room comprises a first state and a second state. The utility model discloses a PCR laboratory with dual-purpose type room of changing clothes has realized that the personnel are pertinence respectively when getting into the PCR laboratory through the district of changing clothes and are handled to usual time and epidemic situation time, effectively improves the security performance to the energy can be saved.
Description
Technical Field
The utility model relates to a PCR laboratory field especially relates to a PCR laboratory with dual-purpose type dressing room.
Background
The PCR laboratory is also called a gene amplification laboratory, and the PCR laboratory generally comprises a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area. PCR is a short term for Polymerase Chain Reaction (Polymerase Chain Reaction), a molecular biology technique used to amplify specific DNA fragments and can be used as a special DNA copy in vitro. The virus content in the body of a patient can be rapidly mastered by a DNA gene tracking system, and the accuracy of the system reaches the nanometer level. For example, the method can accurately detect the quantity, duplication and infection of hepatitis B virus in the body of a patient, the intensity of infection, necessary medicine taking and abnormal liver function, can judge which antiviral medicine the patient is most suitable for using in time and judge the curative effect of the medicine, and provides reliable inspection basis for clinical treatment.
In the process of implementing the embodiments of the present invention, the inventor finds that at least the following defects exist in the background art:
in a normal state, when an operator needs to enter each zone of a PCR laboratory during a conventional PCR project, the operator usually enters a changing zone from a clean zone for buffering and changing, then enters a general corridor, and finally enters each zone. In epidemic situations, such as Severe Acute Respiratory Syndrome (SARS), avian influenza infection, Middle East Respiratory Syndrome (MERS), and novel coronavirus pneumonia (COVID-19), the infection is strong, and when people enter the PCR laboratory, the people are easy to bring in pollutants to pollute the PCR laboratory, which affects the performance of tests in each area. However, the existing PCR laboratory can not realize the separate treatment of personnel in the ordinary period and the epidemic situation period when the personnel enter the PCR laboratory through the dressing area.
SUMMERY OF THE UTILITY MODEL
The embodiment of the application solves the technical problem that in the prior art, when a person enters each zone of the PCR laboratory through a dressing zone, the person cannot respectively process the contaminants brought by the person in the PCR laboratory in the ordinary period and the epidemic situation period to prevent the contamination, and the person respectively deals with the contaminants when entering the PCR laboratory through the dressing zone to effectively improve the safety performance.
To achieve the above object, embodiments of the present application provide a PCR laboratory having a dual-purpose changing room, including: an entry zone, the entry zone comprising: a clean room; the preparation room is communicated with the clean room through a door body; and a changing room, the changing room comprising: the first dressing room is communicated with the preparation room through a first door body; the air shower room is communicated with the first dressing room through a second door body; the second changing room is communicated with the air shower room through a third door body; the special PCR walkway is communicated with the first dressing room through a fourth door body, and the special PCR walkway is communicated with the second dressing room through a fifth door body; the reagent preparation area is communicated with the PCR special passage through a door body and is configured to prepare a storage reagent, split charging the reagent and prepare a main reaction mixed solution; the sample preparation area is communicated with the PCR special passage through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into an amplification reaction tube and synthesize cDNA; a gene amplification region, wherein the gene amplification region is communicated with the PCR dedicated walkway through a gate body and is configured to amplify DNA or cDNA; and a product analysis region, wherein the product analysis region is communicated with the PCR dedicated walkway through a gate body, and the product analysis region is configured to measure an amplified fragment; the dressing room comprises a first state and a second state, the second door body and the fifth door body are closed in the first state, and personnel enter the PCR special walkway through the clean room, the preparation room, the first door body, the first dressing room and the fourth door body in sequence; and in the second state, the fourth door body is closed, and personnel sequentially pass through the clean room, the preparation room, the first door body, the first dressing room, the second door body, the air shower room, the third door body, the second dressing room and the fifth door body to enter the PCR special walkway.
In some embodiments, the first door body is an air tight door.
In some embodiments, the second door body is an air tight door.
In some embodiments, the third door body is an air tight door.
In some embodiments, the fourth door body is an air tight door.
In some embodiments, the fifth door body is an air tight door.
In some embodiments, the reagent preparation zone comprises: the reagent preparation buffer room is communicated with the PCR special passage through a door body; and the reagent preparation room is communicated with the reagent preparation buffer room through a door body and is configured to prepare a stored reagent, subpackage the reagent and prepare a main reaction mixed solution.
In some embodiments, the sample preparation zone comprises: the sample preparation buffer room is communicated with the PCR special passage through a door body; and the sample preparation room is communicated with the sample preparation buffer room through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into the amplification reaction tube and synthesize cDNA.
In some embodiments, the gene amplification region comprises: the gene amplification buffer room is communicated with the PCR special walkway through a door body; and a gene amplification chamber, wherein the gene amplification chamber is communicated with the gene amplification buffer chamber through a door body, and the gene amplification chamber is configured to amplify DNA or cDNA.
In some embodiments, the product analysis zone comprises: a product analysis buffer room communicated with the PCR special passage through a door body; and the product analysis room is communicated with the product analysis buffer room through a door body and is configured to measure the amplified fragments.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:
the PCR laboratory with dual-purpose dressing room provided by the embodiment of the application comprises a clean room, a preparation room and a dressing room through an entrance area, a special PCR walkway, a reagent preparation area, a sample preparation area, a gene amplification area and a product analysis area, wherein the entrance area comprises the clean room, the preparation room and the dressing room, the dressing room comprises a first dressing room, a wind showering room and a second dressing room, the first dressing room is communicated with the preparation room through a first door body, the wind showering room is communicated with the first dressing room through a second door body, the second dressing room is communicated with the wind showering room through a third door body, the special PCR walkway is communicated with the first dressing room through a fourth door body, and the special PCR walkway is communicated with the second dressing room through a fifth door body. When a PCR project is carried out in an epidemic situation, the dressing room can be in a second state, the fourth door body can be closed, personnel can enter the special walk for PCR through the clean room, the preparation room, the first door body, the first dressing room, the second door body, the air shower room, the third door body, the second dressing room and the fifth door body in sequence, enter the first dressing room after buffer preparation of the clean room and the preparation room, can carry out air shower in the air shower room after dressing change of the first dressing room, then enter the second dressing room, can carry out secondary dressing or preparation, finally enter the special walk for PCR through the fifth door body, can enter each area, can carry out secondary dressing and air shower, can effectively remove dust, hair chips and the like carried by the entering personnel, can prevent each area of a PCR laboratory from being polluted, and effectively improves the safety performance. When carrying out conventional PCR project in ordinary times, can make the change clothes room be in first state, make second door body and fifth door body seal, personnel pass through clean room in proper order, prepare the room, the first door body, first change clothes room, the special pavement of PCR is got into to the fourth door body, get into first change clothes room after clean room, the buffering of preparation room is prepared, can directly get into the special pavement of PCR after changing clothes in first change clothes room, then can get into each district, need not to drench room and second change clothes room through the wind, it is more convenient not only to save personnel's walking flow, and need not to open the wind and drench the room, the energy can be saved.
Therefore, the PCR laboratory with the dual-purpose dressing room effectively solves the technical problem that in the prior art, when a person enters each zone of the PCR laboratory through a dressing area, the person cannot be respectively treated in a normal period and an epidemic situation period so as to prevent the pollution caused by pollutants brought by the person in the PCR laboratory, and realizes respective targeted treatment when the person enters the PCR laboratory through the dressing area in the normal period and the epidemic situation period, so that the safety performance is effectively improved, and energy is saved.
Drawings
The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
In the drawings:
FIG. 1 is a plan layout view of a PCR laboratory with a dual-purpose dressing room according to an embodiment of the present application.
In the figure:
100. a PCR laboratory with a dual-purpose dressing room; 10. entering a zone; 11. a clean room; 12. a preparation room; 13. a dressing room; 131. a first dressing room; 134. a first door body; 132. a wind showering room; 135. a second door body; 133. a second dressing room; 136. a third door body; 20. a PCR dedicated walkway; 137. a fourth door body; 138. a fifth door body; 30. a reagent preparation zone; 40. a sample preparation zone; 50. a gene amplification region; 60. a product analysis zone; 31. a reagent preparation buffer chamber; 32. a reagent preparation room; 41. a sample preparation buffer room; 42. a sample preparation room; 51. a gene amplification buffer; 52. during gene amplification; 61. a product analysis buffer; 62. product analysis room.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
It is noted that, unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In the present invention, unless otherwise specified, the use of directional words such as "upper and lower" is generally in reference to the orientation shown in the drawings, or to the vertical, perpendicular or gravitational orientation; likewise, for ease of understanding and description, "left and right" are generally to the left and right as shown in the drawings; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself, but the above directional terms are not intended to limit the present invention.
In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In order to solve the technical problem that in the prior art, personnel cannot respectively treat the PCR laboratory aiming at the ordinary time and the epidemic situation time when entering each zone of the PCR laboratory through a dressing zone so as to prevent the PCR laboratory from being polluted by pollutants brought by the personnel, the embodiment of the application provides the PCR laboratory with the dual-purpose dressing room.
The present invention will be further explained with reference to the accompanying drawings.
As shown in fig. 1, the present embodiment provides a PCR laboratory 100 with a dual-purpose locker room, and the PCR laboratory 100 with the dual-purpose locker room includes an entry area 10, a PCR-dedicated lane 20, a reagent preparation area 30, a sample preparation area 40, a gene amplification area 50, and a product analysis area 60.
The access area 10 includes a clean room 11, a preparation room 12, and a dressing room 13.
The clean room 11 is a first space into which a person enters and can be buffered.
The preparation room 12 is communicated with the clean room 11 through a door body, and preliminary preparation can be performed.
The changing room 13 includes a first changing room 131, a wind showering room 132, a second changing room 133, a first door 134, a second door 135, a third door 136, a fourth door 137, and a fifth door 138.
The first changing room 131 is communicated with the preparation room 12 through the first door 134, and the first changing room 131 can perform changing.
The air shower room 132 is communicated with the first dressing room 131 through a second door 135, and the air shower room 132 is configured to perform air shower on the person. The air shower room 132 can be any air shower room clean shed in the prior art, when a person is disinfected in a first changing room 131, such as changing hands, soaking feet and the like, and enters a second changing room through the air shower room 132, the air shower room is internally provided with an initial-effect and high-efficiency filter, and dust such as dust, flock and the like attached to the person is removed through powerful clean air flow, so that the clean requirement of entering each area of a PCR laboratory can be ensured. The air shower room 132 may be provided with an air shower device indoors, so that the air shower device can perform air shower on the person.
The second dressing room 133 is communicated with the air shower room 132 through the third door 136, so that secondary dressing preparation can be performed, the cleanness degree is further improved, and the safety performance is improved.
The PCR dedicated walkway 20 is a public corridor which enters the reagent preparation area 30, the sample preparation area 40, the gene amplification area 50 and the product analysis area 60, the PCR dedicated walkway 20 is communicated with the first changing room 131 through the fourth door body 137, and the PCR dedicated walkway 20 is communicated with the second changing room 133 through the fifth door body 138.
The reagent preparation area 30 is communicated with the PCR dedicated passage 20 through a door body, and the reagent preparation area 30 is configured to prepare a stored reagent, split-package the reagent, and prepare a main reaction mixed solution.
The sample preparation area 40 is communicated with the PCR dedicated passage 20 through a door body, and the sample preparation area 40 is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA.
The gene amplification region 50 is communicated with the PCR dedicated walkway 20 through a gate body, and the gene amplification region 50 is configured to amplify DNA or cDNA.
The product analysis region 60 is communicated with the PCR dedicated passage 20 through a gate body, and the product analysis region 60 is configured to measure an amplified fragment.
The dressing room 13 includes a first state and a second state, in the first state, the second door 135 and the fifth door 138 are closed, that is, the air shower room 132 and the second dressing room 133 are closed, while the first door and the fourth door can be opened and closed, and personnel can enter the PCR dedicated walkway 20 through the clean room 11, the preparation room 12, the first door 134, the first dressing room 131 and the fourth door 137 in sequence; in the second state, the fourth door 137 is closed, and the first door, the second door, the third door, and the fifth door are all opened and closed, so that the personnel can enter the PCR aisle 20 through the clean room 11, the preparation room 12, the first door 134, the first dressing room 131, the second door 135, the air shower room 132, the third door 136, the second dressing room 133, and the fifth door 138 in sequence.
The PCR laboratory with the dual-purpose dressing room provided in the embodiment of the present application includes an entrance area 10, a PCR dedicated aisle 20, a reagent preparation area 30, a sample preparation area 40, a gene amplification area 50, and a product analysis area 60, wherein the entrance area includes a clean room 11, a preparation room 12, and a dressing room 13, and the dressing room 13 includes a first dressing room 131, a wind showering room 132, and a second dressing room 133, and the first dressing room 131 is communicated with the preparation room 12 via a first door 134, the wind showering room 132 is communicated with the first dressing room 131 via a second door 135, the second dressing room 133 is communicated with the wind showering room 132 via a third door 136, the PCR dedicated aisle 20 is communicated with the first dressing room 131 via a fourth door 137, and the PCR dedicated aisle 20 is communicated with the second dressing room 133 via a fifth door 138. When a PCR project is carried out in an epidemic situation, the dressing room 13 can be in a second state, the fourth door 137 can be closed, personnel can enter the PCR special walkway 20 through the clean room 11, the preparation room 12, the first door 134, the first dressing room 131, the second door 135, the air shower room 132, the third door 136, the second dressing room 133 and the fifth door 138 in sequence, enter the first dressing room 131 after buffer preparation through the clean room 11 and the preparation room 12, can carry out air shower in the air shower room 132 after dressing change of the first dressing room 131, then enter the second dressing room 133, can carry out secondary dressing change or preparation, and finally enter the PCR special walkway 20 through the fifth door 138, so that the dressing room can enter each zone, can carry out secondary dressing change and air shower, can effectively remove dust, such as swarf and the like carried on the personnel, can prevent each zone of the PCR laboratory from being polluted, and effectively improve the safety performance. When a conventional PCR project is carried out at ordinary times, the dressing room 13 can be in a first state, the second door body 135 and the fifth door body 138 are closed, personnel sequentially pass through the clean room 11, the preparation room 12, the first door body 134, the first dressing room 131 and the fourth door body 137 to enter the special PCR walkway 20, enter the first dressing room 131 after buffer preparation of the clean room 11 and the preparation room 12, directly enter the special PCR walkway 20 after dressing change of the first dressing room 131, and then enter each area without passing through the air shower room 132 and the second dressing room 133, so that the walking flow of the personnel is saved, the air shower room is not required to be opened, and energy is saved. Therefore, the PCR laboratory with the dual-purpose dressing room effectively solves the technical problem that in the prior art, when a person enters each zone of the PCR laboratory through a dressing area, the person cannot be respectively treated in a normal period and an epidemic situation period so as to prevent the pollution caused by pollutants brought by the person in the PCR laboratory, and realizes respective targeted treatment when the person enters the PCR laboratory through the dressing area in the normal period and the epidemic situation period, so that the safety performance is effectively improved, and energy is saved.
In this embodiment, the first door 134 is an airtight door, which may be a medical airtight door, and can effectively ensure the relative sealing between the first dressing room and the preparation room when the first door is closed. The first door 134 may also be an airtight sliding door, which is a sliding door assembly that integrates airtightness, sound insulation, heat preservation, pressure resistance, dust prevention, fire prevention, and radiation protection.
In this embodiment, the second door 135 is an airtight door, which may be a medical airtight door, and can effectively ensure the relative sealing between the first dressing room and the air shower room when the second door 135 is closed. The second door body 135 may also be an airtight sliding door, which is a sliding door set integrating airtightness, sound insulation, heat preservation, pressure resistance, dust prevention, fire prevention, and radiation protection.
In this embodiment, the third door 136 is an airtight door, which may be a medical airtight door, and can effectively ensure the relative sealing between the air shower room and the second dressing room when the third door 136 is closed. The third door body 136 may also be an airtight sliding door, which is a sliding door assembly integrating airtightness, sound insulation, heat preservation, pressure resistance, dust prevention, fire prevention and radiation protection.
In this embodiment, the fourth door 137 is an airtight door, which may be a medical airtight door, and can effectively ensure the relative sealing between the first dressing room and the PCR lane 20 when the fourth door 137 is closed. The fourth door body 137 may also be an airtight sliding door, which is a sliding door set integrating airtightness, sound insulation, heat preservation, pressure resistance, dust prevention, fire prevention, and radiation prevention.
In this embodiment, the fifth door 138 is an airtight door, which may be a medical airtight door, and can effectively ensure the relative sealing between the second dressing room and the PCR-dedicated aisle 20 when the fifth door 138 is closed. The fifth door body 138 may also be an airtight sliding door, which is a sliding door set integrating airtightness, sound insulation, heat preservation, pressure resistance, dust prevention, fire prevention, and radiation protection.
In this embodiment, the reagent preparing section 30 includes a reagent preparing buffer compartment 31 and a reagent preparing compartment 32.
The reagent preparation buffer room 31 is communicated with the special PCR walkway 20 through a door body, and the reagent preparation buffer room 31 is a buffer space for a person to enter the reagent preparation room 32 from the special PCR walkway 20. The air pressure of the reagent preparation buffer compartment 31 may be 0 PA.
The reagent preparation room 32 is communicated with the reagent preparation buffer room 31 through a door body, and the reagent preparation room 32 is configured to prepare a stored reagent, split the reagent and prepare a main reaction mixed solution. The reagents and materials for specimen preparation are directly transported to the reagent preparing room 32, and the reagent raw materials are stored in the reagent preparing room 32. The reagent preparation room 32 can be equipped with a 2-8 ℃ refrigerator and a-80 ℃ refrigerator. The area of the room may be 15m2～20m2. The pressure gradient of the reagent preparation chamber 32 may be in a relatively positive pressure state (may be +10PA) to prevent the outside air containing nucleic acid aerosol from entering and causing pollution. A reagent preparing space may be provided in the reagent preparing room 32A conditioner configured to regulate temperature and humidity within the reagent preparation chamber.
In this embodiment, the sample preparation area 40 includes a sample preparation buffer compartment 41 and a sample preparation compartment 42.
The sample preparation buffer room 41 is communicated with the PCR special passage 20 through a door body. The sample preparation buffer room 41 is a buffer space for a person to enter the sample preparation room 42 from the PCR dedicated walkway 20. The air pressure of the sample preparation buffer 41 may be 0 PA.
The sample preparation room 42 is communicated with the sample preparation buffer room 41 through a door body, and the sample preparation room 42 is configured to store clinical specimens, extract nucleic acids, store nucleic acids, add nucleic acids to the amplification reaction tube, and synthesize cDNA. The sample preparation room 42 may be equipped with a 2-8 ℃ refrigerator and a-20 ℃ refrigerator. The sample preparation room 42 is equipped with a biosafety cabinet for performing operations for extracting nucleic acids. To avoid repeated cycling of the extracted nucleic acids within the cabinet, resulting in cross-contamination between samples, and false positive results, the sample preparation booth 42 must be equipped with a biosafety cabinet of type B2. The vertical air flow of the working area of the biological safety cabinet comes from a laboratory, and the exhausted air is directly exhausted to the outside after being filtered by the high-efficiency filter and is not allowed to return to the safety cabinet and the laboratory. The area of the sample preparation compartment 42 may be 25m2～30m2In the meantime. The pressure gradient in the sample preparation bay 42 is desirably positive with respect to the adjacent area (which may be-10 PA) to avoid aerosol contamination from the adjacent area into the local area. A sample preparation room air conditioner may be disposed in the sample preparation room 42 and configured to regulate the temperature and humidity within the sample preparation room.
In this embodiment, the gene amplification region 50 includes a gene amplification buffer room 51 and a gene amplification room 52.
The gene amplification buffer room 51 is communicated with the PCR special passage 20 through a door body. The gene amplification buffer room 51 is a buffer space for people to enter the gene amplification room 52 from the PCR dedicated walkway 20. The gas pressure in the gene amplification buffer 51 may be 0 PA.
The gene amplification room 52 communicates with the gene amplification buffer room 51 through a gate body, and the gene amplification room 52 is configured to amplify DNA or cDNA. In addition, of prepared DNA template and synthesized cDNA (from sample preparation cell 42)Addition and preparation of a reaction mixture from the main reaction mixture (from the reagent preparing chamber 32) may be performed in the gene amplification chamber 52. In nested PCR assays, the reaction tube must usually be opened after the first round of amplification, so nested amplification has a high risk of contamination, and the second sample addition must be performed in the gene amplification chamber 52. The gene amplification room 52 is equipped with the core instrument of the PCR laboratory: as the PCR instrument, for example, two types of PCR instruments, ABI7500 and ABI9700, can be used. The area of the gene amplification chamber 52 can be controlled to be 15m2～20m2. The pressure gradient of the gene amplification chamber 52 is required to be negative pressure with respect to the adjacent region (the gene amplification chamber 52 may be-15 PA) to avoid the escape of aerosol from the region. To avoid contamination by aerosols, unnecessary movement within the region should be minimized. The gene amplification room 52 may be provided therein with a gene amplification room air conditioner configured to regulate the temperature and humidity inside the gene amplification room 52.
In this embodiment, product analysis zone 60 includes a product analysis buffer compartment 61 and a product analysis compartment 62.
The product analysis buffer room 61 is communicated with the PCR special passage 20 through a door body. The product analysis buffer room 61 is a buffer space for personnel to enter the product analysis room 62 from the PCR dedicated walkway 20. The pressure in product analysis buffer 61 may be 0 PA.
The product analysis room 62 communicates with the product analysis buffer room 61 through a door, and the product analysis room 62 is configured to measure the amplified fragment. The area of the product analysis room 62 can be controlled to be 15m 2-20 m2. The product analysis chamber 62 is the most important source of contamination of the amplification product, and therefore the pressure gradient in this region is required to be negative with respect to the adjacent region (the product analysis chamber 62 may be-20 PA) to prevent diffusion of the amplification product from this region to other regions. A product analysis room air conditioner is provided in the product analysis room 62, and is configured to adjust the temperature and humidity inside the product analysis room 62.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the creative work should be covered within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.
Claims (10)
1. A PCR laboratory with a dual purpose changing room, comprising:
an entry zone, the entry zone comprising:
a clean room;
the preparation room is communicated with the clean room through a door body; and
a changing room, the changing room comprising:
the first dressing room is communicated with the preparation room through a first door body;
the air showering room is communicated with the first dressing room through a second door body and is configured to be used for air showering for the personnel; and
the second changing room is communicated with the air shower room through a third door body;
the special PCR walkway is communicated with the first dressing room through a fourth door body, and the special PCR walkway is communicated with the second dressing room through a fifth door body;
the reagent preparation area is communicated with the PCR special passage through a door body and is configured to prepare a storage reagent, split charging the reagent and prepare a main reaction mixed solution;
the sample preparation area is communicated with the PCR special passage through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into an amplification reaction tube and synthesize cDNA;
a gene amplification region, wherein the gene amplification region is communicated with the PCR dedicated walkway through a gate body and is configured to amplify DNA or cDNA; and
a product analysis region, wherein the product analysis region is communicated with the PCR dedicated walkway through a gate body, and the product analysis region is configured to measure an amplified fragment;
the dressing room comprises a first state and a second state, the second door body and the fifth door body are closed in the first state, and personnel enter the PCR special walkway through the clean room, the preparation room, the first door body, the first dressing room and the fourth door body in sequence; and in the second state, the fourth door body is closed, and personnel sequentially pass through the clean room, the preparation room, the first door body, the first dressing room, the second door body, the air shower room, the third door body, the second dressing room and the fifth door body to enter the PCR special walkway.
2. The PCR laboratory with dual-purpose dressing room according to claim 1, wherein:
the first door body is an airtight door.
3. The PCR laboratory with dual-purpose dressing room according to claim 1, wherein:
the second door body is an airtight door.
4. The PCR laboratory with dual-purpose dressing room according to claim 1, wherein:
the third door body is an airtight door.
5. The PCR laboratory with dual-purpose dressing room according to claim 1, wherein:
the fourth door body is an airtight door.
6. The PCR laboratory with dual-purpose dressing room according to claim 1, wherein:
the fifth door body is an airtight door.
7. The PCR laboratory with two-purpose changing room of claim 1, wherein the reagent preparation area comprises:
the reagent preparation buffer room is communicated with the PCR special passage through a door body; and
the reagent preparation room is communicated with the reagent preparation buffer room through a door body and is configured to prepare a stored reagent, split-package the reagent and prepare a main reaction mixed solution.
8. The PCR laboratory with dual-purpose changing room of claim 1, wherein the sample preparation area comprises:
the sample preparation buffer room is communicated with the PCR special passage through a door body; and
the sample preparation room is communicated with the sample preparation buffer room through a door body and is configured to store clinical samples, extract nucleic acids, store the nucleic acids, add the nucleic acids into the amplification reaction tube and synthesize cDNA.
9. The PCR laboratory with two-purpose changing room of claim 1, wherein said gene amplification region comprises:
the gene amplification buffer room is communicated with the PCR special walkway through a door body; and
and the gene amplification chamber is communicated with the gene amplification buffer chamber through a door body and is configured to amplify DNA or cDNA.
10. The PCR laboratory with dual-purpose changing room of claim 1, wherein said product analysis region comprises:
a product analysis buffer room communicated with the PCR special passage through a door body; and
a product analysis room, wherein the product analysis room is communicated with the product analysis buffer room through a door body, and the product analysis room is configured to measure the amplified fragments.
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| CN202021435877.0U CN212773666U (en) | 2020-07-20 | 2020-07-20 | PCR laboratory with dual-purpose type dressing room |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114607181A (en) * | 2022-04-12 | 2022-06-10 | 北京金域医学检验实验室有限公司 | Movable shelter PCR laboratory with optimized spatial layout |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114607181A (en) * | 2022-04-12 | 2022-06-10 | 北京金域医学检验实验室有限公司 | Movable shelter PCR laboratory with optimized spatial layout |
| CN114607181B (en) * | 2022-04-12 | 2022-08-30 | 北京金域医学检验实验室有限公司 | Movable shelter PCR laboratory with optimized spatial layout |
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