CN111657140B - Method for producing hybrid rice seeds of indica rice and japonica rice - Google Patents

Abstract

The invention relates to the field of rice hybrid seed production, and particularly provides a seed production method of indica-japonica hybrid rice. The seed production method provided by the invention is designed aiming at improving the yield of hybrid rice seeds 'Jiayou Zhongke No. 3', the sterile line 'Jia 66A' is taken as a female parent, and the restorer line 'Zhongke Jiahui 1293' is taken as a male parent for hybrid production, wherein gibberellin and Aoxi bath are applied to adjust the flowering time of the female parent during the ear-drawing of the female parent, so that the female parent is promoted to bloom in advance, the pollination probability of the male parent is improved, the seed production yield of the indica-japonica hybrid rice 'Jiayou Zhongke No. 3' is effectively improved, and the seed production technical method of the japonica non-indica-glutinous hybrid rice is enriched.

Description

Method for producing hybrid rice seeds of indica rice and japonica rice

Technical Field

The invention relates to the field of production of hybrid rice seeds, in particular to a seed production method of indica-japonica hybrid rice.

Background

The prior indica-japonica hybrid rice generally has the problems of low seed production yield and unstable seed production yield. The main reason is that the yield of hybrid seeds is low because indica-japonica hybrid is interspecific hybrid, and the production quantity of seeds of the type is influenced. However, with the increase of the market demand of rice, the total amount of corresponding hybrid seeds needs to be increased, the unit yield of production of indica-japonica hybrid seeds is improved, and the establishment of a seed production high-yield and high-efficiency cultivation mode and a standardized system is urgent.

Jiayou Zhongke No. 3 is single season indica-japonica hybrid rice bred by Jiaxing agriculture science institute (institute) in Zhejiang province, China academy of sciences genetics and developmental biology institute, Taizhou province agriculture limited company, has a certified number of Zhe examined rice 2016014, and is suitable for planting japonica rice regions in the middle and lower reaches of Yangtze river. The Jiayou Zhongke No. 3 is a hybrid rice seed produced by using a sterile line 'Jia 66A' as a female parent and a restorer line 'Zhongke Jiahui 1293' as a male parent, and the seed yield is low, however, the prior art has no technical scheme capable of effectively solving the problem.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a seed production method of indica-japonica hybrid rice, which solves the problem of low seed yield of 'Jiayou Zhongke No. 3' hybrid rice in the prior art.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

a method for producing hybrid rice seeds of indica-japonica hybrid rice comprises using sterile line 'Jia 66A' as female parent and restorer line 'Zhongke Jiahui 1293' as male parent to produce hybrid rice seeds 'Jiayou Zhongke No. 3';

wherein, gibberellin and Osei are applied when the female parent shoots ears.

Furthermore, gibberellin and Oseltia are applied when 5% -10% of female parents are spilt.

Further, the amount of gibberellin administered is: 1.5-2.5g of gibberellin with the content of 3% of active ingredients is applied to each mu.

Further, the application amount of oxcibath is as follows: applying 28-32ml oxwestern bath to each mu.

Further, gibberellin and oxter were continuously administered for 2-4 days.

Furthermore, when 5-10% of the male parents are ear-plucked, 1.5-2.5g of gibberellin with the effective component content of 3% is applied to each mu, and 3.5-4.5g of gibberellin with the effective component content of 3% is applied to each mu after every other day.

Furthermore, the seeding dosage of the female parent is 1.6-2 kg/mu, and the seeding dosage of the male parent is 0.3-0.5 kg/mu.

Further, the florescence of the male parent and the female parent meet by adjusting seeding.

Further, the parental ratio is 2: (8-10).

Further, the seed production method also comprises fertilizer and water management;

preferably, the application amount of the base fertilizer per mu comprises 23-27kg of compound fertilizer, 8-12kg of nitrogen fertilizer and 0.3-0.7kg of boron fertilizer;

preferably, the compound fertilizer comprises 16-16-16;

preferably, the nitrogen fertilizer comprises urea;

preferably, the boron fertilizer comprises boron;

preferably, 6-10 kg/mu of urea is independently applied to the male parent and the female parent after transplanting for 6-8 days.

Further, the method also comprises the step of supplementary pollination;

preferably, the rice plants with different forms from the female parent are cut off in the whole growth period, and the male parent is cut off after pollination is finished.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a method for producing seeds of indica-japonica hybrid rice, which is designed aiming at improving the yield of hybrid rice seeds 'Jiayou Zhongke No. 3', a sterile line 'Jia 66A' is taken as a female parent, and a restorer line 'Zhongke Jiahui 1293' is taken as a male parent for hybrid production, wherein gibberellin and Aoshi bath are applied to regulate the flower of the female parent when the female parent spiles, so that the female parent is promoted to bloom in advance, the pollination probability of the male parent is improved, the seed production yield of the indica-japonica hybrid rice 'Jiayou Zhongke No. 3' is effectively improved, and the seed production technical method of the japonica non-indica-glutinous hybrid rice is enriched.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer.

Unless otherwise defined, technical and scientific terms used herein have the same meaning as is familiar to those skilled in the art. In addition, any methods or materials similar or equivalent to those described herein can also be used in the present invention.

The invention provides a seed production method of indica-japonica hybrid rice, which is designed aiming at improving the yield of hybrid rice seeds 'Jiayou Zhongke No. 3', a sterile line 'Jia 66A' is taken as a female parent, a restorer line 'Zhongke Jiahui 1293' is taken as a male parent for hybrid production, wherein gibberellin and Aoshi are applied to adjust the flower of the female parent when the female parent spiles, the female parent is promoted to bloom in advance, the pollination probability of the male parent is improved, the seed production yield of the indica-japonica hybrid rice 'Jiayou Zhongke No. 3' is effectively improved, and the seed production technical method of the japonica non-indica hybrid rice 'Jiayou hui' type indica-japonica hybrid rice is enriched. The method has the characteristics of high and stable yield and the like, effectively improves the seed production yield, and has important reference significance for solving the problem of difficult seed production of indica-japonica hybrid rice.

It can be understood that, for the seed production effect, the purity of the parent and the female parent used in the invention reaches more than 99.5 percent, and the parent and the female parent are purified once every 3 years. The seed production and isolation are carried out when the seeds are planted, the safe isolation distance is more than 500 meters, or the seed production and isolation are carried out at the interval of more than 20 days from the heading and flowering period of the parents.

In some embodiments, it is preferred that when 5% to 10% of the female parents are spiked, gibberellin and Oselt administration is started; the amount of gibberellin administered is preferably: 1.5-2.5g of 3% gibberellin is applied to each mu. The amount of oxcet bath applied is preferably: applying 28-32ml oxwest bath to each mu. The mode of administering gibberellin and Oselt is preferably 2-4 consecutive days.

The inventor finds that the flowering time of the female parent can be effectively advanced by the matched use of gibberellin and Oseltia, the pollination probability of the male parent and the female parent is increased after the flowering time of the male parent and the female parent is coordinated and unified, and the seed yield is improved. Preferably, the dynamic young ear differentiation of the parent is concerned at any time in the young ear differentiation stage, and the flowering stage is adjusted in time. The female parent of the variety is japonica rice, the sword leaves are short, the phimosis is light, and the variety is sensitive to gibberellin. When the female parent spikes 5% -10% in the field, 2 g of 3% gibberellin and 30ml of Osbeck are applied to each mu, and then the Osbeck is continuously applied for 3 days at the same dosage, so that the spiking of the female parent is promoted, and the pollen activity is improved. When the ear of the male parent is 5% -10% in the field, 2 g of 3% gibberellin is sprayed for the first time in each mu, and 4 g is sprayed after every other day to pull up the male parent, which is beneficial to pollination.

In some embodiments, 5% to 10% of the paternal spikes are applied with 1.5 to 2.5g of 3% gibberellin per acre, and 3.5 to 4.5g of 3% gibberellin per acre after every other day. The gibberellin is applied to the male parent, so that the male parent can be promoted to bloom, the male parent is guaranteed to bloom and concentrate, and pollination is facilitated.

In some embodiments, the seed yield can be further improved by defining the seeding mode, seeding time, parent-parent row ratio, water and fertilizer management, supplementary pollination, timely harvest and the like of the parents and the parents.

For the seeding method, the following scheme is preferred: the seeding amount of the female parent is 1.6-2 kg/mu, and the seeding amount of the male parent is 0.3-0.5 kg/mu.

For the sowing time, the female parent and the male parent are two different rice varieties, and if the two rice varieties are planted simultaneously, the flowering phases cannot meet, so that the flowering phases of the male parent and the female parent can be synchronized through different sowing phases of the male parent and the female parent. For example, in Zhejiang, the female parent is suitable for sowing in 6 months and 1 day, the sowing beginning period is 92-95 days, and the ear is taken at the beginning of 9 months; the first-stage male parent seeding period is 6 months and 20, the second-stage male parent seeding period is 6 months and 27, the female parent is 21-27 days earlier than the male parent, and the ear is extracted at the beginning of 9 months. The interval between the 2 nd stage and the 1 st stage of the male parent is preferably 7 days.

It should be noted that the flowering time of the parent and the female parent can be adjusted to the same time period by adjusting the sowing time, so as to achieve the meeting of the flowering time. However, even if the flowering phases meet, if the flowering time of the two parents is too different, the pollination condition of the female parent can be seriously influenced, and for the sterile line Jia 66A and the restorer line Zhongke Jiahui 1293, the flowering time of the male parent is earlier than that of the female parent in the same day, so that the pollination probability can be obviously improved by advancing the flowering time of the female parent. Therefore, the invention adopts gibberellin and Aoxi bath to treat female parents.

For the parental line ratio, the parental line ratio is preferably 2: (8-10). The parents and the female parents can be planted with more parents to improve the effective spike number per mu. The female parent 'Jia 66A' is japonica rice, the plant is short and compact, so the row spacing of the plant is preferably 17 x 17 cm. The male parent is indica-japonica hybrid rice, the plant type is high, the flourishing property is good, the stem is thick and strong, therefore, the plant spacing is 20 cm, the double-row transplanting is carried out, the middle walkway is 60 cm, the male parent is transplanted at intervals in the 2 period, and enough space is reserved for the male parent to grow.

For fertilizer and water management, the base fertilizer preferably adopts a balanced (16-16-16) compound fertilizer, urea and boron fertility, and the application amount of the base fertilizer per mu comprises 23-27kg of the compound fertilizer, 8-12kg of the nitrogen fertilizer and 0.3-0.7kg of the boron fertilizer. Wherein, the compound fertilizer is preferably 16-16-16, the nitrogen fertilizer is preferably urea, and the boron fertilizer is preferably boron. After the male parent and the female parent are transplanted for 6-8 days, 6-10 kg/mu of striking root fertilizer urea is preferably applied respectively.

For auxiliary pollination, the auxiliary pollination is preferably carried out in the ear-pulling and flowering period of the parents by adopting a tractor rope pulling mode, the length of the rope is 50-80 m, and the normal weather in the flowering period is 10: 30-11: 00 pulling the rope for more than 3 times. Also can choose the mode supplementary pollination that unmanned aerial vehicle drove the powder.

For timely harvesting, rice plants with different forms from the female parent are cut off in the whole growth period, and the male parent is cut off after pollination is finished. For example, rice plants with different forms from 'Jia 66A' are cut off in the whole growth period, including field falling grains and parent own mixed plants, the male parent is cut off in time after pollination is finished, the situation of field maturing rate is checked before harvesting, and abnormally maturing plants are cut off. Harvesting and warehousing 25 days after the flowers are contained and the powder is removed.

The invention is further illustrated by the following specific examples, which, however, are to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.

Example 1 Performance of different Agents on maternal flowering-time modulation

This example studies the effect of different administration modes of different agents on female parent flowering time, as shown in table 1 below, wherein the female parent flowering time is also shown in table 1:

TABLE 1

Note: flowering time (agent unit g/mu or ml/mu) for each cell group.

From the flowering time of the female parent, the drug treatment scheme of Aoxi bath and 3% gibberellin in the No. 6 cell can obviously advance the flowering time of the female parent compared with other modes, so that the pollination probability of the female parent is greatly improved, and the seed yield is improved.

Example 2

The method for producing hybrid seeds of indica-japonica hybrid rice Jiayou Zhongke No. 3 in Jiaxing comprises the following steps:

s1, preparing in the early stage before seed production:

parent purification: the purity of parent seeds reaches more than 99.5 percent, and the high-purity parent seeds can improve the field impurity removal efficiency and improve the seed quality.

Safety isolation: the distance of the field safety isolation is required to be more than 500 meters, or the distance of the field safety isolation and the heading flowering period of the father and mother parents is more than 20 days, so that other pollen is prevented from being mixed.

Fertilizing a seedling bed: 15kg of compound fertilizer per mu and 5kg of urea per mu.

Seed soaking: soaking seeds for 36 hours by 500 times of strong chlorine, and accelerating germination after cleaning with clear water.

S2 timely sowing in proper time and in proper amount

Seeding rate: female parent of 1.8 kg/mu and male parent of 0.4 kg/mu. The seedlings are sowed thinly and strengthened and planted with tillers.

And (3) sowing period: the female parent in Zhejiang area is suitable for sowing in 6 months and 1 day, 21-27 days earlier than the male parent, and the ear sprouting of the parent is ensured at the beginning of 9 months. The interval between the 2 nd stage and the 1 st stage of the male parent is preferably 7 days.

S3, parent-parent row ratio: the female parent of Jiayou Zhongke No. 3 is japonica rice, the plant is short and compact, so the row spacing of the plant is preferably 17 x 17 cm. The male parent is indica-japonica hybrid rice, the plant type is high, the flourishing property is good, the stem is thick and strong, therefore, the plant spacing is 20 cm, the double-row transplanting is carried out, the middle walkway is 60 cm, the male parent is transplanted at intervals in the 2 period, and enough space is reserved for the male parent to grow. The parental row ratio is 2: 8 to 10. The parents and the female parents can be planted with more parents to improve the effective spike number per mu.

S4, fertilizer and water management: the base fertilizer adopts 25kg of balanced (16-16-16) compound fertilizer, 10kg of urea and 0.5kg of boron. 8kg of striking root fertilizer urea is applied 7 days after transplanting.

S5, adjusting flowering phase: 2 g of gibberellin with the concentration of 3% and 30ml of Aoxi bath are sprayed per mu when the female parents with the concentration of 5% -10% in the field are spilt, and then the gibberellin and the Aoxi bath are continuously sprayed for 3 days with the same dosage, so that the complete spiling of the female parents is promoted, and the activity of pollen is improved. When the ear of the male parent is 5-10%, the first time 2 g of 3% gibberellin is sprayed and 4 g is sprayed every other day to pull up the male parent, which is beneficial to pollination.

S6, supplementary pollination: in the flowering period of the male parent and the female parent, a tractor rope pulling mode is adopted for auxiliary pollination, the length of the rope is 50-80 m, and the normal weather in the flowering period is 10 every day: 30-11: 00 pulling the rope for more than 3 times.

S7, removing impurities in the field, harvesting and warehousing: rice plants with the patterns different from 'Jia 66A' are cut off in the whole growth period, including field falling grains and parent own mixed plants, the male parents are cut off in time after pollination is finished, the harvester is prevented from being involved in mixing in the later period, the field maturing rate condition is checked before harvesting, and the abnormal maturing plants are cut off. And (5) harvesting the hybrid seeds in time 25 days after the female parent is filled with the flower powder, and drying (sun drying) the hybrid seeds for storage.

Other operations were unchanged and the performance of the production was explored for different maternal densities, the results are shown in table 2 below:

TABLE 2 yield performance of female parent at different densities

Comparative example 1

General seed production method for Jiayou Zhongke No. 3

S1, sowing seeds in different periods

Seeding rate: female parent 1 kg/mu, male parent 0.5 kg/mu.

And (3) sowing period: the female parent is sowed in Zhejiang area at 27 days in 5 months, the male parent is sowed at 20 days in 6 months in the first stage, and the male parent at 28 days in 6 months in the second stage. Stage 2 was 8 days apart from stage 1.

S2, parent-parent row ratio: the female parent of Jiayou Zhongke No. 3 is japonica rice, and the row ratio of the male parent to the female parent is 2: 6, plant row spacing of 24 x 18cm, male parent double-parental plug and female parent double-parental plug.

S3, adjusting flowering phase: spraying 2 g of 3% gibberellin per mu when the 5% -10% female parent in the field shoots, then spraying 1 time every other 1 day, and then spraying 1 time every other 2 days. When the ear of the male parent is 5-10%, the first time 2 g of 3% gibberellin is sprayed per mu, and 2 g is sprayed every other day to pull up the male parent, which is beneficial to pollination.

Test examples

Hybrid rice is planted in Xiuzhou, Changshan and Haining respectively by the technical schemes of the example 2 and the comparative example 1, the hybrid rice is continuously sown for three years, the seed production yield is counted, and the results are shown in the following table 3.

TABLE 3 expression of the yields of different seed production schemes in various regions

Note: the related unit is kg/mu

While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims (12)

1. A method for producing hybrid rice seeds of indica rice and japonica rice is characterized in that hybrid rice seeds 'Jiayou Zhongke No. 3' are produced by taking a sterile line 'Jia 66A' as a female parent and a restorer line 'Zhongke Jiahui 1293' as a male parent;

wherein gibberellin and Oseltia is applied when 5% -10% of female parents are spilt;

single administration amounts of gibberellins were: applying 1.5-2.5g of gibberellin with the effective component content of 3% per mu;

the application amount of the oxter bath is as follows: applying 28-32ml of Aoxi bath to each mu;

gibberellin and oxter were administered continuously for 2-4 days.

2. The method for producing seeds of claim 1, wherein 1.5-2.5g of gibberellin with an effective component content of 3% is applied to each mu when 5% -10% of male parents are spilt, and 3.5-4.5g of gibberellin with an effective component content is applied to each mu every other day.

3. The method for producing seeds of claim 2, wherein the seeding dosage of the female parent is 1.6-2 kg/mu, and the seeding dosage of the male parent is 0.3-0.5 kg/mu.

4. The method for producing seeds of claim 3 wherein the seeding time of the female parent is 1 day at 6 months, the seeding time of the first male parent is 20 days at 6 months, the seeding time of the second male parent is 27 days at 6 months, and the male parent and the female parent have the same flowering time by adjusting the seeding time according to the seeding time when the male parent and the female parent take the ear at the beginning of 9 months.

5. The method for seed production according to claim 4, wherein the parental row ratio is 2: (8-10).

6. The method for producing seeds of claim 5, characterized in that the method for producing seeds further comprises fertilizer and water management, wherein the application amount of the base fertilizer per mu is 23-27kg of compound fertilizer, 8-12kg of nitrogen fertilizer and 0.3-0.7kg of boron fertilizer.

7. The method for producing seed according to claim 6, wherein the compound fertilizer comprises compound fertilizer 16-16-16.

8. The method for producing seed according to claim 6, wherein the nitrogen fertilizer comprises urea.

9. The method for seed production according to claim 6, wherein the boron fertilizer comprises boron acetate.

10. The method for producing seed according to claim 6, characterized in that 6-10 kg/acre of urea is independently applied to the male parent and the female parent after 6-8 days of transplantation.

11. The method for producing seed according to claim 6, characterized by further comprising the step of supplementary pollination.

12. The method for producing seed of claim 11 wherein the rice plant with a pattern different from that of the female parent is excised in the whole growth period, and the male parent is excised after pollination.