CN111632019A - Silk fibroin skin beautifying gel and preparation method thereof - Google Patents

Abstract

The invention discloses a silk fibroin skin beautifying gel and a preparation method thereof, wherein the silk fibroin skin beautifying gel is prepared from the following raw materials in parts by weight: 5-8% of silk fibroin nanoemulsion, 0.3-1.0% of thickening agent, 0.1-0.5% of amine auxiliary agent, 0.1-0.5% of probiotics, 5.0-10.0% of proteoglycan, 2.0-3.0% of prebiotics, 0.1-0.25% of humectant sodium hyaluronate, 0.5-1% of nano silver ions, 0.3-1.0% of promoter, 0.3-0.5% of anti-allergic agent essential oil and the balance of pure water, the silk fibroin nanoemulsion is hydrolyzed to generate silk peptide which can inhibit the generation of skin melanin, vitreous color can promote the generation and construction of protein polysaccharide in extracellular matrix to maintain the delicate and compact skin, in addition, lactobacillus and chlorella extract/lactic acid mixture can synergistically regulate the pH value of private parts, sodium hyaluronate can retain skin moisture and increase skin luster, nano silver particles can strongly sterilize and have strong permeability, the gel prepared by the invention can enhance the activity of skin cells, relieve various inflammation symptoms of pudendum and achieve the aims of moistening skin and balancing skin microecology.

Description

Silk fibroin skin beautifying gel and preparation method thereof

Technical Field

The invention relates to a nursing gel, in particular to a silk fibroin skin beautifying gel and a preparation method thereof.

Background

Women private parts are in moist and muggy environment for a long time, bacteria are easy to breed, so that red swelling, pruritus or inflammation are generated, vaginal flora imbalance and microecological balance problems seriously affect the life quality of women, women in a sub-health state use some nursing products, mild common female gynecological diseases can be relieved and treated, a firm defense system is built for self health, besides medicinal private part nursing gel aiming at treatment, many types of private part nursing gel for daily cleaning and health care are derived, the novel private part nursing gel can clean and moisten private parts, and the requirement of daily maintenance and nursing is met.

The private skin is delicate and sensitive, the irritation components such as alcohol and spice contained in the ordinary private nursing gel can cause irritation to the skin, in addition, some products which are claimed to whiten the private parts are on the market, the private parts are turned to be tender by adding tender red, the principle of the product is similar to dyeing, namely the original color is covered and dyed to be pink, so the effect duration of the product is short, the skin allergy is easily caused, the product is more harmful to women in pregnancy, lactation and menstrual period, and the metabolism is even influenced, so the design of the low-sensitivity and safe private nursing gel is necessary.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a silk fibroin skin beautifying nursing gel and a preparation method thereof.

The technical scheme adopted by the invention for solving the technical problems is as follows:

the silk fibroin skin beautifying gel is prepared from the following raw materials in parts by weight: 5-8% of silk fibroin nanoemulsion, 0.3-1.0% of thickening agent, 0.1-0.5% of amine auxiliary agent, 0.1-0.5% of probiotics, 5.0-10.0% of proteoglycan, 2.0-3.0% of prebiotics, 0.1-0.25% of humectant sodium hyaluronate, 0.5-1% of nano silver ions, 0.3-1.0% of accelerant, 0.3-0.5% of anti-allergy agent essential oil and the balance of pure water.

The thickening agent is

Ultrez 21 polymer.

The amine assistant is aminomethyl propanol.

The probiotic is lactobacillus.

The proteoglycan is a vitreous chromogen.

The prebiotics is a mixture of chlorella extract and lactic acid in a weight ratio of 1:5-5: 1.

The promoter is essential oil of radix Angelicae sinensis.

The anti-allergy agent is agilawood essential oil.

A method of making the gel comprising the steps of:

(1) firstly, the weight fractions are

Adding the Ultrez 21 polymer and pure water with the weight percentage of 80% into a main stirring kettle, and stirring for 5-10 minutes at the rotating speed of 50-60r/min to prepare a component A;

(2) adding the silk fibroin nanoemulsion, the lactobacillus, the boswellin, the angelica essential oil, the agilawood essential oil and the chlorella extract/lactic acid mixture in parts by weight into an emulsion stirrer, stirring for 5-10 minutes at a rotating speed of 50-60r/min until the mixture is uniformly mixed, and keeping the temperature below 45 ℃ to obtain a component B;

(3) adding the hyaluronic acid with the weight percentage and pure water with the weight percentage of 20% into a stirrer, stirring and dissolving at the rotating speed of 50-60r/min until the hyaluronic acid is clear and transparent, and preparing a component C;

(4) firstly, adding the component B into a main stirring kettle, and continuously stirring for 20-30 minutes at a rotating speed of 50-60 r/min; then adding the component C into a main stirring kettle, and stirring for 5-10 minutes at a rotation speed of 50-60 r/min; then adding the nano silver ions with the weight fraction into the main stirring kettle, uniformly stirring for 10-15 minutes at a rotation speed of 50-60 r/min; then adding the aminomethyl propanol with the weight fraction into a main stirring kettle, keeping the rotating speed of 50-60r/min, stirring for 20-30 minutes, uniformly stirring, standing, and discharging.

The invention has the beneficial effects that: the fibroin nano-emulsion disclosed by the invention can generate silk peptide after hydrolysis, can inhibit the generation of skin melanin, has good skin care and moisturizing effects, can promote the generation and construction of protein polysaccharide in extracellular matrix due to vitreous color, prevent the loss of skin water, maintain the tenderness and tightness of skin, can synergistically regulate the pH value of a private part, improve the micro-ecological balance of the skin, can absorb 1000 times of water by sodium hyaluronate, retain the water of the skin, increase the skin luster, can strongly sterilize by nano silver particles, has strong permeability, and can effectively activate and promote the growth of tissue cells, so that the prepared gel can enhance the activity of skin cells, relieve various inflammation symptoms of the pudendum, fulfill the aim of moisturizing the skin and the micro-ecological balance of the skin, and bring effective moisturizing, moisturizing and moisturizing effects to the delicate skin at the private part, And (5) a protection effect.

Detailed Description

The following examples further illustrate the practice and advantages of the present invention, and should not be construed as limiting the scope of the invention.

In the present example, the temperature and pressure are not particularly emphasized, and both are normal temperature and normal pressure.

A silk fibroin skin beautifying gel comprises the following components by weight: 5-8% of silk fibroin nanoemulsion, 0.3-1.0% of thickening agent, 0.1-0.5% of amine auxiliary agent, 0.1-0.5% of probiotics, 5.0-10.0% of proteoglycan, 2.0-3.0% of prebiotics, 0.1-0.25% of humectant sodium hyaluronate, 0.5-1% of nano silver ions, 0.3-1.0% of accelerant, 0.3-0.5% of anti-allergy agent essential oil and the balance of pure water.

The silk fibroin nanoemulsion is silk fibroin processed by a nanotechnology, and the preparation method of the silk fibroin nanoemulsion comprises the following steps: adding silk fibroin solution with molecular weight of below 40kDa into oil phase, mixing uniformly at a ratio of 1:20 to obtain colostrum solution, subjecting the colostrum solution to homogeneous active microjet to obtain silk fibroin nanoemulsion, hydrolyzing silk fibroin to generate silk peptide, and making the silk peptide have effects of inhibiting skin melanin generation and caring skin and keeping moisture.

The thickening agent is

Ultrez 21 polymer (produced by Baishida, Shenzhen, Lu, USA) is a lipophilic modified cross-linked polyacrylate polymer, has short flow property and high viscosity property, and has thickening, stabilizing and suspending properties for various personal care products.

The amine auxiliary agent is aminomethyl propanol, and mainly acts as a pH regulator in cosmetics and skin care products.

The probiotics are lactobacillus which is obtained from plants and separated from rice made in Japan, is known as the most important probiotic member in normal flora of vagina and plays a key role in maintaining self-cleaning and resisting infection of vagina.

The proteoglycan is vitreous chromogen which is a glycoprotein mixture extracted from beech xylose and has the following chemical name: hydroxypropyl tetrahydropyrane triol, which is a vitreous cause, promotes the production and construction of protein polysaccharides in the extracellular matrix after entering the skin. These molecules absorb water, make the matrix gel-like, increase the firmness of the cells and skin, and can also serve as channels for the passage of reparative molecules.

The prebiotics is a mixture of chlorella extract and lactic acid with the weight ratio of 1:5-5:1, and the preparation method of the chlorella extract is a high-pressure hot water extraction method: heating at 120 deg.C for 40min, stirring at 10000 r/min for 30min, and filtering with 0.2 μm filter membrane to obtain Chlorella extract containing Chlorella Growth Factor (CGF) which has effects of inducing interferon, stimulating macrophage, T cell and B cell in human body defense and immune tissue, and has Chlorella chlorophyll content 5-6 times that of Spirulina and strong toxin adsorption effect.

The humectant sodium hyaluronate is a skin conditioner, is an acidic mucopolysaccharide, naturally exists in corneal skin, can absorb 1000 times of moisture of the corneal skin by weight, achieves the effects of retaining skin moisture and preventing the moisture from losing through epidermis, realizes barrier repair when the skin is damaged, enables the skin not to feel dry after being used, increases luster, can improve skin nutrition metabolism, enables the skin to be soft and smooth, removes wrinkles, increases elasticity and prevents aging, and is well absorbed through skin while moisturizing.

The nano silver particles (produced by Guangzhou Bokung chemical engineering Co., Ltd.) as a natural antibacterial agent has the characteristics of broad-spectrum antibacterial sterilization, no drug resistance, strong sterilization and strong permeability, and can also effectively activate and promote the growth of tissue cells and promote healing.

The promoter is essential oil of radix Angelicae sinensis (produced by Yilong chemical Co., Ltd., Guangzhou), contains volatile oil ferulic acid, can be absorbed transdermally, and can change physicochemical properties and normal physiological structure of skin to different degrees after acting on skin, and increase permeability.

The anti-allergic agent is agilawood essential oil (produced by Guangzhou Guowei Biotechnology limited), the agilawood essential oil is precious agilawood volatile oil obtained by distilling agilawood, and is a natural perfume.

Example 1:

the embodiment is prepared from the following raw materials in parts by weight: 5% of silk fibroin nanoemulsion, 0.3% of thickening agent, 0.1% of amine auxiliary agent, 0.1% of probiotics, 5.0% of proteoglycan, 2.0% of prebiotics, 0.1% of humectant sodium hyaluronate, 0.5% of nano silver ions, 0.3% of accelerant, 0.3% of anti-allergy agent essential oil and the balance of pure water.

The preparation method of the embodiment comprises the following steps:

(1) firstly, the weight fractions are

Adding the Ultrez 21 polymer and pure water with the weight percentage of 80% into a main stirring kettle, and stirring for 5-10 minutes at the rotating speed of 50-60r/min to prepare a component A;

(2) adding the silk fibroin nanoemulsion, the lactobacillus, the boswellin, the angelica essential oil, the agilawood essential oil and the chlorella extract/lactic acid mixture in parts by weight into an emulsion stirrer, stirring for 5-10 minutes at a rotating speed of 50-60r/min until the mixture is uniformly mixed, and keeping the temperature below 45 ℃ to obtain a component B;

(3) adding the hyaluronic acid with the weight percentage and pure water with the weight percentage of 20% into a stirrer, stirring and dissolving at the rotating speed of 50-60r/min until the hyaluronic acid is clear and transparent, and preparing a component C;

(4) firstly, adding the component B into a main stirring kettle, and continuously stirring for 20-30 minutes at a rotating speed of 50-60 r/min; then adding the component C into a main stirring kettle, and stirring for 5-10 minutes at a rotation speed of 50-60 r/min; then adding the nano silver ions with the weight fraction into the main stirring kettle, uniformly stirring for 10-15 minutes at a rotation speed of 50-60 r/min; then adding the aminomethyl propanol with the weight fraction into a main stirring kettle, keeping the rotating speed of 50-60r/min, stirring for 20-30 minutes, uniformly stirring, standing, and discharging.

Example 2:

the embodiment is prepared from the following raw materials in parts by weight: 8% of silk fibroin nanoemulsion, 1.0% of thickening agent, 0.5% of amine auxiliary agent, 0.5% of probiotics, 10.0% of proteoglycan, 3.0% of prebiotics, 0.25% of humectant sodium hyaluronate, 1% of nano silver ions, 1.0% of accelerant, 0.5% of anti-allergy agent essential oil and the balance of pure water.

The preparation method of this example is the same as example 1.

And (4) testing standard:

and (3) testing the corrosion resistance: united states pharmacopoeia USP32(51)

The strains of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans and aspergillus niger with standard assigned numbers are inoculated into the nursing gel respectively prepared in the examples 1-2, then the survival number of each strain is respectively checked on the 7 th day, the 14 th day and the 28 th day, the lower the survival number is, the better the antiseptic antibacterial effect of the antiseptic is, the bacteria are reduced by 99% on the 14 th day, the bacteria are not reproduced on the 28 th day, the fungi are not reproduced on the 14 th day and the 28 th day, and the nursing gel prepared in the examples 1-2 accords with the test indexes of the USP32 (51).

Quality performance inspection test: GB 15979 + 2002 hygienic standards for disposable sanitary articles:

the nursing gel prepared in the example 1-2 meets the test index of GB 15979-2002.

Actual use test of volunteers:

and (3) moisture retention performance test: 10 volunteers were randomly divided into 2 groups, test group 1 and test group 2, respectively, test group 1 using the care gel prepared in example 1, and test group 2 using the care gel prepared in example 2, each at 1.0g/30cm²The change in moisture content at the test site was detected using a control on the arm of the volunteer. The test results were as follows:

	average skin moisture content before use	Average skin moisture content after application
			Test set 1	36.2％	57.4％
Test set 2	36.8％	59.8％

The tabulated data shows that the gels prepared in examples 1-2 have good moisturizing properties.

And (3) testing the bacteria breeding performance: 10 volunteers, test group 1 respectively, test group 1 used the care gel prepared in example 1 to apply 0.1 of the additive amount to the face of the volunteer. After 4 weeks, forehead strains were collected, and the test results were as follows:

and (3) measuring the lactic acid bacteria at the forehead to be bifidobacteria, enterococcus, lactobacillus, lactococcus, leuconostoc lactis, pediococcus and streptococcus, summing the number of the bacteria at the forehead, and subtracting the number of the probiotics in the original smearing liquid to obtain a value which is the value of the lactic acid bacteria promoted to be generated by the nursing gel.

	Probiotic amount of original coating liquid	Amount of probiotic on face
			0 week	1	1
4 weeks	1	≧2

The tabulated data shows that the gel prepared in example 1 promotes lactic acid bacteria growth.

Testing the PH adjusting performance: 10 volunteers were randomly divided into 2 groups, test group 1 and test group 2, respectively, test group 1 using the care gel prepared in example 1, and test group 2 using a commercially available care gel product at 1.0g/30cm, respectively²The control was washed on the arm of the volunteer for 4 weeks and the PH content of the test sites was checked against the control. The test results were as follows:

	skin pH value before use	Skin pH after application
			Test set 1	6.0	4.3
Test set 2	6.0	6.2

From the tabulated data, it is seen that the treatment gel of example 1 can adjust skin pH to a mild acid, and further, that test group 1 exhibits a dramatic reduction in redness over 2 weeks of application of the gel of example 1, indicating that the gel of example 1 has a soothing and soothing effect.

One complete skin irritation test: the skin irritation index of the test substance to white rabbits is 0, and the skin irritation intensity is nonirritant.

Vaginal mucosa irritation test: the test article was non-irritating to the vaginal mucosa of rabbits.

And the toxicological tests are detected by a third-party detection mechanism, namely, a microbiological analysis and detection center in Guangdong province.

Comprehensive use inspection and test: volunteers are recruited in a gynecological clinic of a certain hospital, and the conditions of the recruited volunteers are as follows: 200 female volunteers were recruited and randomly divided into 4 groups of 50 persons each, namely a test group 1, a test group 2, a test group 3 and a test group 4, wherein the test group 1 used the care gel prepared in example 1, the test group 2 used the care gel prepared in example 2, the test group 3 used a commercially available care gel product, the test group 4 used a commercially available moisturizing care gel, and the number of samples was measured in two weeks.

The nursing gel used in the test has the marked substances such as trademarks and the like torn off, so that volunteers do not know the nursing gel used by the volunteers, and the volunteers need to provide own use feeling to experimenters after finishing each time. The use experience comprises: whether the composition is comfortable after use, whether the composition can improve colony balance, whether the composition is moisturized or not, whether the composition is not dry after use, and whether the composition has elasticity after use are scored, wherein each item is 10 points in full, and the better the feeling is, the higher the score is. The test results are shown in the following table:

compared with the commercially available nursing gel, the silk fibroin skin care gel for the private parts has the obvious advantages of moisturizing, moistening, tightening, moistening and itching relieving. Meanwhile, the nursing gel product prepared by the embodiment of the invention enhances the activity of skin cells, relieves various symptoms of pudendum inflammation, has elastic touch after use, achieves the purposes of moistening skin and balancing skin microecology, and comprehensively maintains private skin.

The above embodiments do not limit the scope of the present invention, and those skilled in the art can make equivalent modifications and variations without departing from the overall concept of the present invention.

Claims (9)

1. The silk fibroin skin beautifying gel is characterized by being prepared from the following raw materials in parts by weight: 5-8% of silk fibroin nanoemulsion, 0.3-1.0% of thickening agent, 0.1-0.5% of amine auxiliary agent, 0.1-0.5% of probiotics, 5.0-10.0% of proteoglycan, 2.0-3.0% of prebiotics, 0.1-0.25% of humectant sodium hyaluronate, 0.5-1% of nano silver ions, 0.3-1.0% of accelerant, 0.3-0.5% of anti-allergy agent essential oil and the balance of pure water.

2. A gel according to claim 1, characterized in that said thickener is Carbopol ® Ultrez 21 polymer.

3. The gel of claim 1, characterized in that said amine adjuvant is aminomethyl propanol.

4. The gel according to claim 1, characterized in that said probiotic bacteria are lactobacilli.

5. The gel of claim 1, wherein said proteoglycan is a vitreous chromogen.

6. The gel according to claim 1, characterized in that said prebiotic is a mixture of chlorella extract and lactic acid in a weight ratio of 1:5 to 5: 1.

7. The gel of claim 1, wherein said enhancer is essential oil of angelica sinensis.

8. The gel according to claim 1, characterized in that said anti-sensitiser is agilawood essential oil.

9. A process for preparing a gel according to any one of claims 1 to 8, comprising the steps of:

(1) firstly, adding the Carbopol Ultrez 21 polymer with the weight fraction and the pure water with the weight fraction of 80% into a main stirring kettle, and stirring for 5-10 minutes at the rotating speed of 50-60r/min to prepare a component A;

(2) adding the silk fibroin nanoemulsion, the lactobacillus, the boswellin, the angelica essential oil, the agilawood essential oil and the chlorella extract/lactic acid mixture in parts by weight into an emulsion stirrer, stirring for 5-10 minutes at a rotating speed of 50-60r/min until the mixture is uniformly mixed, and keeping the temperature below 45 ℃ to obtain a component B;

(3) adding the hyaluronic acid with the weight percentage and pure water with the weight percentage of 20% into a stirrer, stirring and dissolving at the rotating speed of 50-60r/min until the hyaluronic acid is clear and transparent, and preparing a component C;

(4) firstly, adding the component B into a main stirring kettle, and continuously stirring for 20-30 minutes at a rotating speed of 50-60 r/min; then adding the component C into a main stirring kettle, and stirring for 5-10 minutes at a rotation speed of 50-60 r/min; then adding the nano silver ions with the weight fraction into the main stirring kettle, uniformly stirring for 10-15 minutes at a rotation speed of 50-60 r/min; then adding the aminomethyl propanol with the weight fraction into a main stirring kettle, keeping the rotating speed of 50-60r/min, stirring for 20-30 minutes, uniformly stirring, standing, and discharging.