CN111602052A - 一种血液检测方法及血液分析系统 - Google Patents
一种血液检测方法及血液分析系统 Download PDFInfo
- Publication number
- CN111602052A CN111602052A CN201980008302.4A CN201980008302A CN111602052A CN 111602052 A CN111602052 A CN 111602052A CN 201980008302 A CN201980008302 A CN 201980008302A CN 111602052 A CN111602052 A CN 111602052A
- Authority
- CN
- China
- Prior art keywords
- blood
- light
- optical
- laser beam
- scattered light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 170
- 210000004369 blood Anatomy 0.000 title claims abstract description 157
- 239000008280 blood Substances 0.000 title claims abstract description 157
- 238000004159 blood analysis Methods 0.000 title claims description 20
- 230000003287 optical effect Effects 0.000 claims abstract description 241
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 207
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 130
- 238000000034 method Methods 0.000 claims abstract description 126
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 103
- 239000002245 particle Substances 0.000 claims abstract description 78
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 71
- 238000012360 testing method Methods 0.000 claims abstract description 55
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 42
- 238000000149 argon plasma sintering Methods 0.000 claims abstract description 14
- 239000012634 fragment Substances 0.000 claims abstract description 11
- 239000000975 dye Substances 0.000 claims description 122
- 230000010287 polarization Effects 0.000 claims description 87
- 210000001995 reticulocyte Anatomy 0.000 claims description 77
- 210000000601 blood cell Anatomy 0.000 claims description 52
- 210000004027 cell Anatomy 0.000 claims description 48
- -1 alkyl glycoside Chemical class 0.000 claims description 45
- 238000006243 chemical reaction Methods 0.000 claims description 43
- 239000007850 fluorescent dye Substances 0.000 claims description 43
- 150000007523 nucleic acids Chemical class 0.000 claims description 41
- 102000039446 nucleic acids Human genes 0.000 claims description 41
- 108020004707 nucleic acids Proteins 0.000 claims description 41
- 239000012528 membrane Substances 0.000 claims description 37
- 210000004698 lymphocyte Anatomy 0.000 claims description 35
- 210000001616 monocyte Anatomy 0.000 claims description 35
- 210000000440 neutrophil Anatomy 0.000 claims description 35
- 230000002438 mitochondrial effect Effects 0.000 claims description 28
- 238000012545 processing Methods 0.000 claims description 28
- 238000010586 diagram Methods 0.000 claims description 26
- 239000013078 crystal Substances 0.000 claims description 20
- 229930182470 glycoside Natural products 0.000 claims description 20
- 150000007524 organic acids Chemical class 0.000 claims description 16
- 239000002736 nonionic surfactant Substances 0.000 claims description 15
- 230000008859 change Effects 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 150000003839 salts Chemical class 0.000 claims description 13
- 210000003714 granulocyte Anatomy 0.000 claims description 12
- 238000004590 computer program Methods 0.000 claims description 10
- MYFATKRONKHHQL-UHFFFAOYSA-N rhodamine 123 Chemical compound [Cl-].COC(=O)C1=CC=CC=C1C1=C2C=CC(=[NH2+])C=C2OC2=CC(N)=CC=C21 MYFATKRONKHHQL-UHFFFAOYSA-N 0.000 claims description 10
- 239000012103 Alexa Fluor 488 Substances 0.000 claims description 9
- 238000005070 sampling Methods 0.000 claims description 8
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 7
- 150000002772 monosaccharides Chemical group 0.000 claims description 7
- 150000004676 glycans Chemical class 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- 229910052783 alkali metal Inorganic materials 0.000 claims description 5
- 210000003470 mitochondria Anatomy 0.000 claims description 5
- XXACTDWGHQXLGW-UHFFFAOYSA-M Janus Green B chloride Chemical compound [Cl-].C12=CC(N(CC)CC)=CC=C2N=C2C=CC(\N=N\C=3C=CC(=CC=3)N(C)C)=CC2=[N+]1C1=CC=CC=C1 XXACTDWGHQXLGW-UHFFFAOYSA-M 0.000 claims description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 4
- 150000002338 glycosides Chemical class 0.000 claims description 4
- 235000005985 organic acids Nutrition 0.000 claims description 4
- 239000002243 precursor Substances 0.000 claims description 4
- 125000000542 sulfonic acid group Chemical group 0.000 claims description 4
- 150000008130 triterpenoid saponins Chemical class 0.000 claims description 4
- 150000005856 steroid saponins Chemical class 0.000 claims description 2
- 230000002934 lysing effect Effects 0.000 abstract description 3
- 206010018910 Haemolysis Diseases 0.000 description 30
- 230000008588 hemolysis Effects 0.000 description 30
- 238000005259 measurement Methods 0.000 description 24
- 210000003979 eosinophil Anatomy 0.000 description 21
- 230000005284 excitation Effects 0.000 description 20
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- 235000002639 sodium chloride Nutrition 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 235000000346 sugar Nutrition 0.000 description 16
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 15
- 229910052799 carbon Inorganic materials 0.000 description 15
- 230000000694 effects Effects 0.000 description 15
- 238000010186 staining Methods 0.000 description 14
- 238000009534 blood test Methods 0.000 description 13
- 239000000980 acid dye Substances 0.000 description 12
- 239000003795 chemical substances by application Substances 0.000 description 11
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- 125000000217 alkyl group Chemical group 0.000 description 10
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 10
- IKEOZQLIVHGQLJ-UHFFFAOYSA-M mitoTracker Red Chemical compound [Cl-].C1=CC(CCl)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 IKEOZQLIVHGQLJ-UHFFFAOYSA-M 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 210000003651 basophil Anatomy 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- 230000009089 cytolysis Effects 0.000 description 7
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 7
- 238000007430 reference method Methods 0.000 description 7
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 6
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 6
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 6
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000012757 fluorescence staining Methods 0.000 description 6
- 230000002949 hemolytic effect Effects 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 238000000386 microscopy Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000012085 test solution Substances 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 239000002738 chelating agent Substances 0.000 description 4
- 230000002452 interceptive effect Effects 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000005192 partition Methods 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- VRYALKFFQXWPIH-PBXRRBTRSA-N (3r,4s,5r)-3,4,5,6-tetrahydroxyhexanal Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)CC=O VRYALKFFQXWPIH-PBXRRBTRSA-N 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 3
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 3
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 3
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 3
- SRBFZHDQGSBBOR-STGXQOJASA-N alpha-D-lyxopyranose Chemical compound O[C@@H]1CO[C@H](O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-STGXQOJASA-N 0.000 description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 3
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 3
- 229940073499 decyl glucoside Drugs 0.000 description 3
- 125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- NLEBIOOXCVAHBD-QKMCSOCLSA-N dodecyl beta-D-maltoside Chemical compound O[C@@H]1[C@@H](O)[C@H](OCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 NLEBIOOXCVAHBD-QKMCSOCLSA-N 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 229930182830 galactose Natural products 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 description 2
- RUVJFMSQTCEAAB-UHFFFAOYSA-M 2-[3-[5,6-dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]benzimidazol-2-ylidene]prop-1-enyl]-3-methyl-1,3-benzoxazol-3-ium;chloride Chemical compound [Cl-].O1C2=CC=CC=C2[N+](C)=C1C=CC=C(N(C1=CC(Cl)=C(Cl)C=C11)CC=2C=CC(CCl)=CC=2)N1CC1=CC=C(CCl)C=C1 RUVJFMSQTCEAAB-UHFFFAOYSA-M 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 235000015165 citric acid Nutrition 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000002847 impedance measurement Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000011090 malic acid Nutrition 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 2
- 238000001579 optical reflectometry Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000007447 staining method Methods 0.000 description 2
- 229930002600 steroidal saponin Natural products 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- VUWCWMOCWKCZTA-UHFFFAOYSA-N 1,2-thiazol-4-one Chemical class O=C1CSN=C1 VUWCWMOCWKCZTA-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 239000012099 Alexa Fluor family Substances 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- JDRSMPFHFNXQRB-CMTNHCDUSA-N Decyl beta-D-threo-hexopyranoside Chemical compound CCCCCCCCCCO[C@@H]1O[C@H](CO)C(O)[C@H](O)C1O JDRSMPFHFNXQRB-CMTNHCDUSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- 229940092714 benzenesulfonic acid Drugs 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003617 erythrocyte membrane Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- ZCTXEAQXZGPWFG-UHFFFAOYSA-N imidurea Chemical compound O=C1NC(=O)N(CO)C1NC(=O)NCNC(=O)NC1C(=O)NC(=O)N1CO ZCTXEAQXZGPWFG-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 150000003628 tricarboxylic acids Chemical class 0.000 description 1
- CEYYIKYYFSTQRU-UHFFFAOYSA-M trimethyl(tetradecyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)C CEYYIKYYFSTQRU-UHFFFAOYSA-M 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/018—Platelets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Ecology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Signal Processing (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
本发明公开了一种血液检测方法，包括：用第一试剂处理血液样本以获得待测试样，所述第一试剂包括溶血剂，所述溶血剂将所述血液样本中的红细胞裂解为其光散射特性显著不同于血小板的碎片；使待测试样中的粒子逐个通过光学检测系统的检测区，获取所述待测试样的光学信息；和根据所述光学信息中的至少两种获得血小板的光学信息。本发明的方法通过裂解血液样本中的红细胞获取血小板计数，并可同时区分白细胞亚群。
Description
PCT国内申请，说明书已公开。
Claims (44)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810402676 | 2018-04-28 | ||
| CN2018104026761 | 2018-04-28 | ||
| PCT/CN2019/084660 WO2019206300A1 (zh) | 2018-04-28 | 2019-04-26 | 一种血液检测方法及血液分析系统 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111602052A true CN111602052A (zh) | 2020-08-28 |
| CN111602052B CN111602052B (zh) | 2021-10-12 |
Family
ID=68294399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980008302.4A Active CN111602052B (zh) | 2018-04-28 | 2019-04-26 | 一种血液检测方法及血液分析系统 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US12092633B2 (zh) |
| EP (1) | EP3789764A4 (zh) |
| CN (1) | CN111602052B (zh) |
| WO (1) | WO2019206300A1 (zh) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022115982A1 (zh) * | 2020-12-01 | 2022-06-09 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析方法、样本分析仪及计算机可读存储介质 |
| CN115015178A (zh) * | 2022-08-05 | 2022-09-06 | 天津迈科隆生物科技有限公司 | 一种光学检测装置及血液分析仪 |
| WO2023125942A1 (zh) * | 2021-12-31 | 2023-07-06 | 深圳迈瑞生物医疗电子股份有限公司 | 血液细胞分析仪、方法以及感染标志参数的用途 |
| TWI848718B (zh) * | 2023-05-23 | 2024-07-11 | 晶鑠科技有限公司 | 血紅素糖化終產物檢測儀器及其檢測容器 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110249223B (zh) * | 2017-02-17 | 2020-10-20 | 深圳迈瑞生物医疗电子股份有限公司 | 血液细胞分析方法及血液细胞分析仪 |
| CN118347920A (zh) * | 2020-05-14 | 2024-07-16 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪及样本分析方法 |
| CN114252386A (zh) * | 2020-09-23 | 2022-03-29 | 深圳迈瑞生物医疗电子股份有限公司 | 样本检测方法和样本分析仪 |
| CN118451329A (zh) | 2021-12-31 | 2024-08-06 | 深圳迈瑞生物医疗电子股份有限公司 | 血液细胞分析仪、方法以及感染标志参数的用途 |
| CN116952805A (zh) | 2022-04-14 | 2023-10-27 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪以及血小板计数方法 |
| CN114636684A (zh) * | 2022-05-20 | 2022-06-17 | 深圳市帝迈生物技术有限公司 | 流式荧光分析设备 |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
| WO1996004544A1 (en) * | 1994-08-01 | 1996-02-15 | Abbott Laboratories | Method and apparatus for performing automated analysis |
| CN1322146A (zh) * | 1999-09-03 | 2001-11-14 | 巴克斯特国际公司 | 具有检测程序的血液处理方法 |
| EP1542008A1 (en) * | 2002-06-24 | 2005-06-15 | Sysmex Corporation | Method of classifying and counting leucocytes |
| CN1834659A (zh) * | 2005-03-17 | 2006-09-20 | 希森美康株式会社 | 试料分析装置和试料分析方法以及血液分析装置 |
| CN1981187A (zh) * | 2004-05-14 | 2007-06-13 | 霍尼韦尔国际公司 | 便携式样本分析仪盒 |
| CN101490547A (zh) * | 2006-07-17 | 2009-07-22 | 海莫库公司 | 血小板的计数 |
| CN102155927A (zh) * | 2011-03-22 | 2011-08-17 | 浙江大学 | 一种基于激光自准直的二维微角度测量装置 |
| CN102230994A (zh) * | 2011-06-29 | 2011-11-02 | 武汉电信器件有限公司 | 法拉第磁光隔离器 |
| CN102243339A (zh) * | 2011-07-04 | 2011-11-16 | 武汉电信器件有限公司 | 光隔离器 |
| CN106525666A (zh) * | 2015-09-14 | 2017-03-22 | 希森美康株式会社 | 血液分析装置、血液分析方法及信息处理装置 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4099917A (en) | 1977-07-21 | 1978-07-11 | Technicon Instruments Corporation | Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles |
| US4882284A (en) | 1987-04-13 | 1989-11-21 | Ortho Pharmaceutical Corporation | Method for quantitating and differentiating white blood cells |
| US5817519A (en) | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| TW379284B (en) | 1996-04-12 | 2000-01-11 | Toa Medical Electronics | Agent for detecting reticulocyte |
| US6114173A (en) | 1997-04-03 | 2000-09-05 | Bayer Corporation | Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood |
| JP2005024472A (ja) * | 2003-07-04 | 2005-01-27 | Sysmex Corp | 幼若血小板測定装置 |
| US7390662B2 (en) * | 2005-11-09 | 2008-06-24 | Beckman Coulter, Inc. | Method and apparatus for performing platelet measurement |
| US7344890B2 (en) | 2005-11-09 | 2008-03-18 | Beckman Coulter, Inc. | Method for discriminating platelets from red blood cells |
| JP4806330B2 (ja) | 2006-10-30 | 2011-11-02 | シスメックス株式会社 | 網状赤血球及び血小板測定用試薬、網状赤血球及び血小板測定用試薬キット並びに網状赤血球及び血小板測定方法 |
| JP4711009B2 (ja) * | 2008-10-16 | 2011-06-29 | ソニー株式会社 | 光学的測定装置 |
| CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
| JP6206404B2 (ja) * | 2012-06-06 | 2017-10-04 | ソニー株式会社 | 微小粒子測定装置におけるデータ補正方法及び微小粒子測定装置 |
| US9176112B2 (en) | 2012-12-31 | 2015-11-03 | Beckman Coulter, Inc. | Systems and methods for platelet count with clump adjustment |
| CN104749144A (zh) * | 2013-12-31 | 2015-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | 血细胞检测试剂及血细胞处理方法和识别方法 |
| EP3258274B1 (en) * | 2016-06-17 | 2019-11-06 | Sysmex Corporation | Method of controlling a blood analyzer for measuring platelets |
| FR3062514A1 (fr) * | 2017-02-02 | 2018-08-03 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Formation de reliefs a la surface d'un substrat |
-
2019
- 2019-04-26 CN CN201980008302.4A patent/CN111602052B/zh active Active
- 2019-04-26 WO PCT/CN2019/084660 patent/WO2019206300A1/zh unknown
- 2019-04-26 EP EP19792205.7A patent/EP3789764A4/en active Pending
-
2020
- 2020-10-27 US US17/081,195 patent/US12092633B2/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
| WO1996004544A1 (en) * | 1994-08-01 | 1996-02-15 | Abbott Laboratories | Method and apparatus for performing automated analysis |
| CN1322146A (zh) * | 1999-09-03 | 2001-11-14 | 巴克斯特国际公司 | 具有检测程序的血液处理方法 |
| EP1542008A1 (en) * | 2002-06-24 | 2005-06-15 | Sysmex Corporation | Method of classifying and counting leucocytes |
| CN1981187A (zh) * | 2004-05-14 | 2007-06-13 | 霍尼韦尔国际公司 | 便携式样本分析仪盒 |
| CN1834659A (zh) * | 2005-03-17 | 2006-09-20 | 希森美康株式会社 | 试料分析装置和试料分析方法以及血液分析装置 |
| CN101490547A (zh) * | 2006-07-17 | 2009-07-22 | 海莫库公司 | 血小板的计数 |
| CN102155927A (zh) * | 2011-03-22 | 2011-08-17 | 浙江大学 | 一种基于激光自准直的二维微角度测量装置 |
| CN102230994A (zh) * | 2011-06-29 | 2011-11-02 | 武汉电信器件有限公司 | 法拉第磁光隔离器 |
| CN102243339A (zh) * | 2011-07-04 | 2011-11-16 | 武汉电信器件有限公司 | 光隔离器 |
| CN106525666A (zh) * | 2015-09-14 | 2017-03-22 | 希森美康株式会社 | 血液分析装置、血液分析方法及信息处理装置 |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022115982A1 (zh) * | 2020-12-01 | 2022-06-09 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析方法、样本分析仪及计算机可读存储介质 |
| WO2023125942A1 (zh) * | 2021-12-31 | 2023-07-06 | 深圳迈瑞生物医疗电子股份有限公司 | 血液细胞分析仪、方法以及感染标志参数的用途 |
| CN115015178A (zh) * | 2022-08-05 | 2022-09-06 | 天津迈科隆生物科技有限公司 | 一种光学检测装置及血液分析仪 |
| TWI848718B (zh) * | 2023-05-23 | 2024-07-11 | 晶鑠科技有限公司 | 血紅素糖化終產物檢測儀器及其檢測容器 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20210102935A1 (en) | 2021-04-08 |
| EP3789764A4 (en) | 2021-06-23 |
| WO2019206300A1 (zh) | 2019-10-31 |
| EP3789764A1 (en) | 2021-03-10 |
| US12092633B2 (en) | 2024-09-17 |
| CN111602052B (zh) | 2021-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111602052B (zh) | 一种血液检测方法及血液分析系统 | |
| US9638621B2 (en) | Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer | |
| US5928949A (en) | Reagent and method for classifying leukocytes by flow cytometry | |
| EP0483116B1 (en) | Method of classifying leukocytes by flow cytometry and reagents used in the method | |
| US20030219850A1 (en) | Method for automatically analyzing nucleated bone marrow cells | |
| CN111656188B (zh) | 试剂、分析血小板的方法及血液细胞分析仪 | |
| US9797824B2 (en) | Method for hematology analysis | |
| EP2705135B1 (en) | Basophil analysis method | |
| EP2972321B1 (en) | System for analyzing white blood cells | |
| US20140038179A1 (en) | Reagent and reagent kit for analysis of immature leukocyte | |
| US5179026A (en) | Method of classifying leukocytes by flow cytometry and reagents used in the method | |
| CN111602046A (zh) | 一种血液分析仪及分析方法 | |
| EP0259833A2 (en) | Reagent and method for classifying leukocytes by flow cytometry | |
| CN114270167A (zh) | 血液检测方法及血液分析系统 | |
| US10215683B2 (en) | Light scatter based apparatus and methods for hematology analysis using only three detectors | |
| CN115201160A (zh) | 一种血液细胞检测方法及分析系统 | |
| Sakata | Reagent characteristics in the XE-2100 NRBC channel | |
| CN115201161A (zh) | 试剂、血液细胞分析方法及分析仪 | |
| CN118258993A (zh) | 用于血液分析的试剂、血液分析方法及血液分析系统 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |