CN111568960A

CN111568960A - Agent for inhibiting and improving gray hair and method for producing same

Info

Publication number: CN111568960A
Application number: CN202010093797.XA
Authority: CN
Inventors: 西村荣美; 木谷敏之; 上野康博
Original assignee: Edem Ltd
Current assignee: Edem Ltd
Priority date: 2019-02-15
Filing date: 2020-02-14
Publication date: 2020-08-25
Also published as: JP2020132547A

Abstract

The invention provides a white hair suppression-improving agent for suppressing and improving white hair and a method for producing the white hair suppression-improving agent. The present invention relates to a white hair suppression/improvement agent containing 1 to 700ppm of amygdalin. The poliosis inhibiting and improving agent preferably contains extract of almond.

Description

Agent for inhibiting and improving gray hair and method for producing same

Technical Field

The present invention relates to a white hair suppression/improvement agent and a method for producing the same.

Background

Conventionally, various agents for suppressing and improving white hair have been reported for the purpose of suppressing and improving white hair of hair (for example, patent documents 1 and 2). However, it is not said that the white hair suppression/improving agent satisfies the market needs in terms of efficacy and the like, and it is necessary to search for a new active ingredient for improving the white hair suppression/improving agent.

Documents of the prior art

Patent document

Patent document 1: japanese patent No. 3660485

Patent document 2: japanese patent No. 4433241

Disclosure of Invention

Technical problem to be solved

Accordingly, an object of the present invention is to provide a white hair suppression-improving agent for suppressing and improving white hair and a method for producing the white hair suppression-improving agent.

Means for solving the problems

The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, have found that the above-mentioned problems can be solved by incorporating a small amount of amygdalin, and have completed the present invention.

Namely, the agent for improving the white hair suppression of the present invention contains 1 to 700ppm of amygdalin.

Preferably, the poliosis inhibiting and improving agent of the present invention contains an extract of almond.

The method for producing the agent for suppressing and improving canities of the present invention comprises incorporating 1 to 700ppm of amygdalin.

Preferably, the method for producing the agent for improving the white hair suppression of the present invention incorporates an extract of almond.

Effects of the invention

According to the present invention, a white hair suppression/improvement agent for suppressing and improving white hair and a method for producing the white hair suppression/improvement agent can be provided.

Drawings

FIG. 1 is a photograph showing the results of a usefulness test conducted by repeatedly applying an almond extract (30% EtOH extract) to C57BL/6N mice irradiated with X-rays, for every 4 mice;

FIG. 2 is a photograph showing the results of a usefulness test conducted by repeatedly applying amygdalin solution using X-ray irradiated C57BL/6N mice, for every 4 mice;

fig. 3 is a photograph of the auricle of each mouse after repeated smearing for one month, showing the results of the study of the side effects of the almond extract solution;

fig. 4 is a photograph of a tissue image (HE staining) of auricles of each group of mice showing the results of the study of side effects of the almond extract.

Detailed Description

The agent for suppressing and improving canities of the present invention contains 1 to 700ppm of amygdalin. The poliosis inhibition and improvement agent can comprise D-amygdalin and L-amygdalin in any proportion, and particularly can contain 1-700 ppm of D-amygdalin. The method for producing amygdalin is not particularly limited, and it may be synthesized by a known and customary synthetic method, or may be purified from a natural product, or may be a commercially available product. As amygdalin, amygdalin contained in an extract of almond can be suitably used.

The amygdalin content in the agent for improving the white hair suppression of the present invention is preferably 2 to 600ppm, more preferably 5 to 550ppm, and still more preferably 10 to 500 ppm. By setting the above range, a more excellent white hair suppression and improvement effect can be obtained. Here, the concentration may be a mixture of D-amygdalin and L-amygdalin, and particularly, the concentration of D-amygdalin may be also used. The purity of D-amygdalin may be, for example, 50% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 98% or more, or 99% or more.

< extract of apricot kernel >

Preferably, the extract of almond (seed of Prunus armeniaca) is an extract of seed (kernel) of apricot. The extract of almond is not particularly limited, and examples thereof include: various solvent extracts, dilutions thereof, concentrated solutions thereof, or solutions of dried powders and dried powders thereof. The extraction method is not particularly limited, and extracts obtained by a known and conventional method can be used.

The extraction solvent is not particularly limited, and may be water, an organic solvent, or a mixed solvent of water and an organic solvent. Examples of the extraction solvent include: for example, water, lower alcohols such as methanol and ethanol, liquid polyols such as propylene glycol and 1, 3-butanediol, lower alkyl esters such as ethyl acetate, hydrocarbons such as benzene and hexane, diethyl ether, acetone and the like are known solvents. These solvents may be used alone in 1 kind, or may be used in combination of 2 or more kinds. Preferably water, a lower alcohol, or a mixed solvent of water and a lower alcohol, more preferably a mixed solvent of water and a lower alcohol, and still more preferably a mixed solvent of water and ethanol.

The obtained extract may be used as it is, or may be further subjected to a treatment such as filtration, if necessary. In addition, for example, the extract may be subjected to partition treatment with water and an organic solvent, or may be eluted by liquid chromatography to recover and use a component having an effect of improving the white hair suppression. These treatments may be combined.

The extraction may be carried out at room temperature, or a heated extraction solvent may be used as in the reflux extraction. Examples of the extraction method using a heated extraction solvent include: a method for extracting semen Armeniacae amarum by soaking in extraction solvent heated to above 50 deg.C.

In addition, amygdalin-degrading enzyme is contained in almond. Since the degradation reaction by this enzyme is very rapid, the amygdalin content is reduced when, for example, almond is immersed in a solvent for extraction, and when the extract liquid is stored, or when a liquid-state poliosis-inhibiting improver is stored. In the present invention, it is important to incorporate a small amount of amygdalin, but if the content is reduced too much by degradation with the enzyme, the effect of improving the white hair suppression may be insufficient, and therefore, it is preferable to perform a treatment such as removal or inactivation of the enzyme to suppress the reduction of amygdalin.

In the present invention, the reduction of amygdalin can be suppressed by performing the extraction step after the steam heat treatment step of heat-treating the almonds in a steam atmosphere. When the steam heat treatment step is performed, an extract having a higher amygdalin content after the extraction step and a lower benzaldehyde content as a degradation product of amygdalin can be obtained as compared with the case of performing other heat treatment. In addition, amygdalin is present in nature mostly as D-body. By carrying out isomerization (D form- > L form) according to the change of heat and pH, the D-amygdalin is reduced, but by carrying out the steam heat treatment step, an extract having a high D-amygdalin content can be produced. Therefore, amygdalin contained in the agent for improving white hair suppression of the present invention may be a mixture of D-amygdalin and L-amygdalin, and 50% or more of the total amount thereof may be D-amygdalin, for example, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 98% or more, or 99% or more may be D-amygdalin.

In the steam heat treatment step, the method of heat-treating the almonds in a steam atmosphere is not particularly limited, and for example, the following methods are preferably used: the plant and water are put into a closed or semi-closed container, and the plant and the water are not contacted, and the plant is heated in the steam generated by the boiling of the water. In addition, a method may be used in which the plant is placed in a closed or semi-closed container and heated, and the plant is heated in steam generated from moisture contained in the plant.

Here, the container forming the closed space is not particularly limited in shape and the like as long as it is made of a material having a property of being impermeable to water vapor or gas and is heat-resistant to pressure generated inside the container by heating. For example, a retort pouch, a can, a bottle, a heating device having a sealed structure, or the like can be used. The container forming the semi-closed space may be made of a material impermeable to water vapor or gas, and may have a structure in which the pressure generated in the container by heating is appropriately released to maintain the internal pressure at normal pressure, and the shape and the like are not particularly limited. For example, a retort pouch, a pot or a pot with a steam port on the lid, a vessel with a pressure release valve, a heating device, or the like, in which 1 to several holes having a diameter of 1 to several mm are opened, can be used. Alternatively, the lid of the pot or jar with the lid may be released and used as a container forming a semi-closed space.

The steam heating treatment is more preferably performed in a semi-closed container. Further, since the hermetically sealed container cannot release the pressure generated inside the container by heating, it is necessary to reduce the pressure before heating, or it is difficult to increase the scale of the manufacturing process by using a pressure resistant container, and therefore, it is preferable to perform the sealing in a semi-hermetically sealed container under normal pressure.

The heating temperature in the steam heating treatment is preferably 40 to 150 ℃, more preferably 70 to 120 ℃, even more preferably about 100 ℃, for example 95 to 105 ℃. When heating is performed under normal pressure, it is preferable to perform heating at a saturated vapor pressure of about 1000 hPa. The treatment at 70 ℃ or lower requires a long time, and scorching may occur at a temperature of 120 ℃ or higher.

The heating time in the steam heating treatment is preferably 3 minutes or more, and more preferably 3 to 90 minutes. Further, the content of amygdalin after extraction is further increased, and therefore, it is more preferably 8 to 50 minutes, and particularly preferably 10 to 40 minutes.

The water vapor content in the gas phase in the vessel in the water vapor heating treatment is preferably 20% or more, more preferably 50% or more, and even more preferably 70% or more of the saturated water vapor content at the heating temperature. For example, it is preferably 120 to 600g/m at 100 ℃ under normal pressure³More preferably 300 to 600g/m³More preferably 420 to 600g/m³. The time required for the treatment is increased when the water vapor content is low, and therefore, the treatment is not only less efficient but also causes the deterioration of the plant due to moisture absorption and the decrease in the amygdalin content.

In the steam heating treatment, when the plant containing amygdalin is disposed so as not to directly contact water, the disposition is not particularly limited, and specific examples thereof include: placing a small amount of water in a stainless steel tank, heating to boil, placing plant containing amygdalin on a metal strainer, placing in the stainless steel tank without direct contact with water, and heating with a dummy cover.

The formulation of the poliosis inhibiting and improving agent of the present invention is not particularly limited, and for example, an extract of almond may be used as it is. In addition, the composition may be in the form of an external preparation for skin, a cosmetic for hair care, or the like, which is mixed with an appropriate base, drug, or the like. Specifically, lotions, latexes, ointments, creams, compresses, gels, oils, films, shampoos, hair oils, conditioners, hair sprays, hair root tonics, hair lotions, aerosols, mousses, and the like can be used. Further, the composition can be used by being incorporated into, for example, soap, bath salt, or the like, in addition to the external preparation described above.

The white hair suppression/improving agent of the present invention may contain commonly used additives such as a moisturizing agent, an oily component, a surfactant, vitamins, a protease, a thickener, a preservative, a powder, an antioxidant, an ultraviolet absorber, an emulsifier, alcohols, a pigment, an aqueous component, fatty acids, a perfume, a refreshing agent, a chelating agent, a pH adjuster, and purified water, in a cosmetic, quasi-drug, pharmaceutical preparation, or the like.

In addition, other components having an effect of improving the white hair suppression may be incorporated in the white hair suppression-improving agent of the present invention within a range not affecting the effect of the present invention.

The agent for suppressing and improving white hair of the present invention can also be used for suppressing and improving white hair of hair other than hair.

In the method for producing the agent for ameliorating leukotrichia of the present invention, amygdalin and an extract of almond are as described above.

[ examples ]

The present invention will be further described in detail by way of examples and comparative examples, but the present invention is not limited to these examples and comparative examples.

< preparation of Almond extract >

The almond extract was prepared by the following method.

< sample >

Sample preparation: apricot seeds (raw material name: almond)

Shape: undisturbed state of shelled seed

Amygdalin content: 5% (obtained by HPLC method)

1L of water was placed in a 20L capacity stainless steel tank under normal pressure, and after a virtual cover, the tank was heated until the water boiled. After boiling, a sample (almond/raw material) was placed on a metal strainer, and placed in the stainless steel tank so as not to directly contact water, and a semi-closed container was formed by virtual masking, and while keeping out the water in the container from drying, the water was continuously heated to boil, and the sample was subjected to a heating treatment for 30 minutes by water vapor generated by the water boiling.

Soaking the treated 1.0kg semen Armeniacae amarum in 5L 30 wt% ethanol at room temperature for 10 days, and extracting to obtain semen Armeniacae amarum extractive solution. The amygdalin concentration is determined by High Performance Liquid Chromatography (HPLC) to prepare various concentrations of the almond extract.

< measurement conditions of HPLC >

A detector: ultraviolet absorption spectrophotometer (detection wavelength 210nm)

Column chromatography: a column of octadecylsilylated silica gel for liquid chromatography (YMC-Pack Pro C18, manufactured by YMC, Ltd.) having an inner diameter of 3.0mm and a length of 250mm was packed in a stainless steel tube having a length of 5 μm

Column temperature: constant temperature of about 10 DEG C

The mobile phase: the mixed solvent of 0.1% phosphoric acid aqueous solution and acetonitrile was used in the mobile phase, and the mixing ratio of 0.1% phosphoric acid aqueous solution and acetonitrile was 90: isocratic elution of 10.

Flow rate: 0.4mL/min

Injection amount: 10 μ L

Preparation of standard solutions and creation of calibration curves: a standard amygdalin (manufactured by wako pure chemical industries, ltd.) was dissolved in a mixture of water and acetonitrile (water: acetonitrile: 90: 10) to prepare a standard solution, and a calibration curve was created from the peak area value in HPLC analysis.

< usefulness test by repeated application of Almond extract (30% EtOH extract) to X-ray irradiated C57BL/6N mice >

The experimental animals used C57BL/6N mice, and mice other than the control group were subjected to X-ray irradiation treatment (5Gy) at 50 days of age, and thereafter, all mice were subjected to hair clipper treatment and depilatory treatment, and repeated application of each test substance was started (application was performed after each depilatory treatment, once a day, 100. mu.L each, for one week continuously, and thereafter, application was performed once a day, 100. mu.L each, for 5 weeks). After one month, the hair clipper treatment and depilating treatment were performed again, and the application was repeated in the same manner for one month, and evaluation was made on the regenerated hair after the 2 nd depilation.

TABLE 1

In fig. 1, photographs of the regenerated hairs of the control (no X-ray irradiation) and the control (X-ray irradiation), and of each of 4 mice repeatedly applied with the above-mentioned test substance are shown. As is clear from FIG. 1, the application of the almond extract solutions of examples 1 to 4 significantly suppressed the development of canities.

< usefulness test by repeatedly applying amygdalin solution to C57BL/6N mice irradiated with X-ray >

TABLE 2

	Treatment (test substance)	Number only
			Reference example 2	X-ray non-irradiation control (50% ethanol)	4
Comparative example 3	X-ray irradiation control (50% ethanol)	4
			Example 5	X-ray irradiation + amygdalin solution (13.3ppm amygdalin) × 1	4
Example 6	X-ray irradiation + amygdalin solution (40ppm amygdalin) × 1	4
			Example 7	X-ray irradiation + amygdalin solution (120ppm amygdalin) × 1	4
Comparative example 4	X-ray irradiation + amygdalin solution (1000ppm amygdalin) × 1	4
			Comparative example 5	X-ray irradiation and bitter apricot seedsGlycoside solution (10000ppm amygdalin) × 1	4

*1: a liquid obtained by dissolving amygdalin produced by DONG JIAO NANG chemical Co., Ltd in 30% ethanol.

In fig. 2, photographs of regenerated hairs of 4 mice each repeatedly applied with the above-mentioned test substance are shown. As can be seen from FIG. 2, the application of the amygdalin solutions of examples 5 to 7 significantly suppressed canities.

< study of side effects of Almond extract >

The following studies were made on the possibility that the scalp background color might be blackened due to the side effect of the almond extract liquid which blackens the hair.

Experimental animals using C57BL/6N mice, 20 μ L of each of the left ears of the 50% ethanol-applied group and the almond extract (40ppm amygdalin) applied group (6 animals per group) was repeatedly applied for one month, and then auricles were visually observed (photographed), and the auricles were collected and subjected to histological study (HE staining). Figure 3 shows a photograph of the pinna of each mouse after one month of repeated application. Fig. 4 shows representative tissue images (HE staining) of each group of pinna. The proportion (%) of the pigmented cells in the epidermal cells in the auricle of each mouse after repeated application for one month is shown in table 3.

As shown in table 3, no significant change was observed even when the almond extract was repeatedly applied for one month as compared with the control.

TABLE 3

As a result of t-testing the proportion of the pigmented cells shown in table 3, no significant difference was observed.

From the above results, it is clear that the agent for improving white hair suppression of the present invention is useful for suppressing and improving white hair.

Specific blending ratios of the white hair suppression-improving agent of the present invention are described below, but the present invention is not limited thereto. In addition, as the almond extract, an almond extract containing 5000ppm of amygdalin was used in the following blending ratio.

< white solid soap >

The components shown in the following table were subjected to mechanical kneading (each component was stirred and mixed in a kneader, taken out from the kneader, cooled to room temperature in a cooling roll, kneaded 2 to 3 times in a bar press for soap, extruded from a cone, press-molded and dried, and subjected to bulk polishing molding) and frame kneading (various components other than additives such as menthol were dissolved by mixing and heating (about 70 ℃), additives were added when cooled to about 50 ℃, mixed and flowed into a frame for frame kneading, taken out from the frame after solidification, dried for 60 to 90 days, press-molded in a press-molding machine, and subjected to bulk polishing) to produce white solid soap.

TABLE 4

Name of raw materials	Amount incorporated (% by mass)
		Triethanolamine laurate	2.0
Sodium laurate (NaLaurus acid)	28.0
		Sodium stearate	54.1
Stearic acid	2.0
		1-menthol	0.5
Pyroglutamic acid menthyl ester	0.1
		Glycerol	0.1
1, 3-butanediol	0.1
		Almond extract	1.0
Jojoba oil	0.1
		Perfume	1.0
Titanium dioxide	0.2
		Ethylenediaminetetraacetic acid Tetrasodium salt	0.05
Tetra sodium hydroxy ethane diphosphonate	0.05
		Purified water	Balance of
Total up to	100

< hair styling liquid >

The hair lotions were prepared by conventional methods at the incorporation ratios given in the table below.

TABLE 5

< shampoo >

Shampoos were made by conventional methods at the following incorporation ratios in the table.

TABLE 6

Name of raw materials	Amount incorporated (% by mass)
		Almond extract	1.00
Peanut extract	1.00
		Sodium polyoxyethylene lauryl ether sulfate solution	20.00
Triethanolamine solution of fatty acid acyl glutamic acid of N-coconut oil	10.00
		Coconut oil fatty acid amidopropyl solution	10.00
Coconut fatty acid diethanolylAmines as pesticides	4.00
		Ethylene glycol distearate	2.00
Cationic cellulose	1.00
		Cationic guar gum	0.20
Polyoxyethylene (10) stearyl ether	1.00
		Methyl polysiloxane	0.50
Propylene glycol	5.00
		EDTA-2 sodium salt	0.30
Paraoxybenzoic acid ester	0.40
		Citric acid	0.20
Perfume	0.30
		Purified water	Balance of
Total up to	100.0

< Care element >

Carpentins were produced by conventional methods at the incorporation ratios in the following table.

TABLE 7

Name of raw materials	Amount incorporated (% by mass)
		Almond extract	1.00
Peanut extract	1.00
		Stearyl trimethyl ammonium chloride	1.00
Distearyldimethylammonium chloride	1.00
		Alkyl trimethyl ammonium chloride	1.50
Polyoxypropylene (20) cetyl ether	0.50
		Polyoxyethylene Monostearate (20) sorbitan	1.00
Cetyl alcohol	1.50
		Stearyl alcohol	1.50
Octyl dodecanol	1.50
		Lanolin derivative (lanolin cation)	0.50
Cationic cellulose	1.00
		Cationic guar gum	0.20
Silicon emulsion	1.00
		Glycerol	2.00
Diglycerol	4.00
		Propylene glycol	1.00
Paraoxybenzoic acid ester	0.20
		Perfume	0.30
Purified water	Balance of
		Total up to	100.0

< ointment (PEG) >

Ointments (PEG) were prepared by conventional methods at the incorporation ratios in the following table.

TABLE 8

< ointment (Paraffin) >

Ointments (paraffin wax) were prepared by a conventional method at the incorporation ratios in the following table.

TABLE 9

Name of raw materials	Amount incorporated (% by mass)
		Liquid paraffin	15.0
Almond extract	1.0
		Vaseline	84.0
Total up to	100.0

< Hair restorer >

The almond extract obtained in the above preparation example was dissolved in 50% ethanol aqueous solution. This solution was used to prepare a hair restorer by mixing with the ingredients shown in the following table.

Watch 10

Name of raw materials	Amount incorporated (% by mass)
		Ginger tincture	1.0
Herba Swertiae Bimaculatae concentrate	1.0
		Carrot concentrated solution	1.0
Peony concentrated solution	1.0
		Almond extract	1.0
Anhydrous ethanol	50.5
		Purified water	Balance of
Total up to	100.0

< hairy root nutrient solution >

According to the recipe (mass%) of the following table, first, dl- α -tocopheryl acetate, isopropyl methylphenol, panthenol, glycyrrhetinic acid, ethanol (99.5%), dodecyl myristate, octyl 2-ethylhexanoate, perfume, menthol were uniformly mixed. Then, mixing herba Swertiae Bimaculatae extractive solution, radix Dauci Sativae extractive solution, 1, 3-butanediol, dipropylene glycol, sodium ascorbyl phosphate, sodium ethylene diamine tetracetate, polyoxyethylene hydrogenated castor oil (50E.O), polyoxyethylene hydrogenated castor oil (20E.O), semen Armeniacae amarum extractive solution, and purified water. Finally, the two solutions are mixed and filtered to fill the mixture, thereby producing a liquid type hair root nutrient solution.

TABLE 11

Name of raw materials	Amount incorporated (% by mass)
		dl- α -tocopheryl acetate	0.1
Isopropyl methyl phenol	0.1
		Panthenol	0.5
Glycyrrhetinic acid	0.1
		Ethanol (99.5%)	40.0
Myristic acid dodecyl ester	0.2
		2-Ethylhexanoic acid octyl ester	1.0
Perfume	0.2
		Menthol crystal	1.0
Herba Swertiae Bimaculatae extractive solution	1.0
		Carrot extract	0.5
1, 3-butanediol	3.0
		Dipropylene glycol	3.0
Ascorbic acid sodium phosphate	0.1
		Ethylenediaminetetraacetic acid sodium salt	0.1
Polyoxyethylene hydrogenated castor oil (50E.O.)	0.7
		Polyoxyethylene hydrogenated castor oil (20E.O.)	0.3
Almond extract	1.0
		Purified water	Balance of
Total up to	100.0

< hairy root nutrient solution >

According to the recipe (mass%) of the following table, first, dl- α -tocopheryl acetate, isopropyl methylphenol, panthenol, ethanol (99.5%), perfume, menthol were uniformly mixed. Then, mixing herba Swertiae Bimaculatae extractive solution, radix Dauci Sativae extractive solution, dipropylene glycol, 3-methyl-1, 3-butanediol, sodium ascorbyl phosphate, sodium ethylene diamine tetracetate, polyoxyethylene polyoxypropylene glycol, glyceryl laurate monolaurate, semen Armeniacae amarum extractive solution, and purified water. Then, the two are mixed and filtered to be used as a stock solution, and finally, the stock solution and a propellant are filled into a can according to a filling formula to manufacture an aerosol type hair root nutrient solution.

TABLE 12

< emulsion >

An emulsion (aerosol product) was produced by adding an alcohol phase to an aqueous phase according to the following formulation (mass%), adjusting a stock solution, putting the stock solution in a can, and filling a gas such as LPG, butane, or the like.

Watch 13

< emulsion >

A weakly acidic emulsion (transparent type) having a pH of 5.5 was prepared by adding an alcohol phase to an aqueous phase according to the formulation (mass%) shown in the following table.

TABLE 14

< emulsion >

An emulsion (white turbid type) of ph7.5 was prepared by adding an alcohol phase to an aqueous phase and emulsifying according to the formulation (mass%) in the following table.

Watch 15

< emulsion >

A 3-layer type emulsion (separation type) of ph6.2 was produced by adding an alcohol phase to an aqueous phase according to the formulation (mass%) in the following table, solubilizing the mixture, and then adding a powder phase.

TABLE 16

< spray lotion >

The spray lotion was produced by a conventional method at the incorporation ratios in the following table.

TABLE 17

Name of raw materials	Amount incorporated (% by mass)
		Purified water	92.69
Almond extract	1.00
		Concentrated glycerin	5.00
Phenoxyethanol	0.30
		Dipropylene glycol	1.00
Hyaluronic acid sodium salt	0.01
		Total up to	100.0

< Aerosol spray >

The aerosol sprays were produced by conventional methods at the incorporation ratios given in the table below.

Watch 18

Name of raw materials	Amount incorporated (% by mass)
		Purified water	91.60
Almond extract	1.00
		1, 3-butanediol	5.00
Phenoxyethanol	0.40
		Dipropylene glycol	2.00
Total up to	100.0

< cleaning agent >

The detergents were manufactured by a conventional method at the incorporation ratios in the following table.

Watch 19

< bath rinse lotion >

The bath rinse was made by conventional methods at the incorporation ratios in the table below.

Watch 20

Name of raw materials	Amount incorporated (% by mass)
		1-menthol	0.5
1-menthyl glyceryl ether	0.1
		Almond extract	1.0
Polyoxyethylene hydrogenated castor oil (60)	1.0
		Anhydrous ethanol	20.0
Carboxyvinyl polymer	0.5
		Methyl polysiloxane	2.0
Triethanolamine	0.3
		Perfume	0.5
Esters of p-hydroxybenzoic acid	30.0
		Water (W)	Balance of
Total up to	100.0

Claims

1. A leukotrichia-suppressing and-ameliorating agent characterized by containing 1 to 700ppm of amygdalin.

2. The agent for suppressing and improving gray hair according to claim 1, which comprises an extract of almond.

3. A method for producing a leukotrichia-suppressing and ameliorating agent, characterized by incorporating 1 to 700ppm of amygdalin.

4. The method for producing a leukotrichia inhibitor/improver as set forth in claim 3, wherein an extract of almond is incorporated.

5. The method for producing a leukotrichia inhibitor/improver according to claim 4, comprising a step of heat-treating almond in a steam atmosphere.