JP5144048B2 - Radical scavenger, antioxidant skin external preparation, and radical scavenging method - Google Patents

Description

  The present invention relates to a radical scavenger useful as an active ingredient of a skin external preparation such as cosmetics. The present invention also relates to an anti-oxidant skin external preparation and a method for eliminating radicals.

  Conventionally, for the purpose of imparting prescribed medicinal effects to skin external preparations such as emulsions, creams, lotions, packs, cleaning agents, dispersions, ointments, solutions, aerosols, patches, poultices, liniments, etc. Various medicinal ingredients are added. In recent years, various causes of skin troubles have been elucidated, and along with this, consumer needs for external preparations for skin, particularly cosmetics, are being subdivided. For example, it is known that active oxygen and free radicals cause damage to living tissues and cells, and thus cause various diseases and aging. In order to improve the skin that was in an environment where active oxygen is generated in the skin tissue, such as stimulation by ultraviolet rays or chemical substances, an agent with excellent scavenging ability against active oxygen and free radicals generated by those causes It is desirable to provide

On the other hand, since the safety to the skin is also important as an active ingredient to be blended in the external preparation for skin, various plant extracts that are agents derived from natural products have been proposed as active ingredients for external preparations for skin. Yes. For example, a moisturizing agent composed of a human lizard extract has been proposed (Patent Document 1).
Japanese Patent No. 3696862

It is an object of the present invention to provide a novel radical scavenger that is highly safe for the skin and useful as an active ingredient of an external preparation for skin.
Moreover, this invention makes it a subject to provide the skin external preparation for antioxidant excellent in the antioxidant effect.
Another object of the present invention is to provide a novel method for eliminating radicals.

In order to solve the above-mentioned problems, the present invention provides a radical scavenger comprising a human lizard extract as an active ingredient.
From another viewpoint, the present invention provides an anti-oxidant skin external preparation containing human lizard extract as an active ingredient; a method for eliminating radicals by supplying human lizard extract.

  ADVANTAGE OF THE INVENTION According to this invention, the novel agent which has the outstanding erasing ability with respect to a radical, especially a superoxide, and a novel method can be provided. Moreover, according to this invention, the skin external preparation for antioxidant excellent in the antioxidant effect can be provided.

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The radical scavenger of the present invention contains, as an active ingredient, an extract of Chloranthus japonicus (Scientific name: Chloranthus japonicus). There is no restriction | limiting about an extraction site | part, and the extract of any parts, such as a root, stem, a leaf, an inflorescence, a fruit, and a seed of a human lizardfish, may be sufficient as the human lizard extract used for this invention. In addition, the radical scavenger of the present invention may be mixed with extracts obtained from two or more places of human lysizuka, or two or more extracts extracted from two or more portions with different solvents. May be used.

  The human squirrel extract is obtained by extracting one or two or more parts such as roots, stems, leaves, inflorescences, fruits, and seeds of the squirrel with a suitable solvent, and distilling off the solvent. These portions may be subjected to an appropriate treatment such as drying, chopping, pressing, or fermentation, followed by an extraction treatment or the like. Extraction can be carried out by immersing human lizard in a solvent at a low temperature or under heating for a predetermined time. The extraction solvent is not particularly limited. For example, water or lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol; ketones such as acetone; ethyl One kind or two or more kinds of organic solvents such as ethers such as ether; esters such as ethyl acetate; and a mixed solvent of these with water can be used.

  The human squirrel extract may be used as it is in a skin external preparation, or may be used after being left to stand for an appropriate period and aged. If necessary, it can be used after further purification treatment such as filtration, deodorization and decolorization with an ion exchange resin or the like within a range not affecting the effect. Further, only a fraction having a high activity can be used by using a separation means such as liquid chromatography.

  Examples of preferred methods for preparing the human lizard extract include human lizards, alcoholic solvents such as hydrous or non-hydrated ethyl alcohol, polyhydric alcohol solvents such as hydrous or non-hydrated 1,3-butylene glycol, or water. The mixture is filtered at room temperature or warmed for 1 to 5 days, filtered, and the filtrate obtained is left to mature for about one week, and the filtrate is filtered again.

Examples of the components in the extract include flavonoid glycosides, phenols, amino acids, triterpenes and the like. The higher the content of the alcohol solvent or polyhydric alcohol solvent in the extraction solvent, the higher the triterpene content.
In the human lizard extract used for the radical scavenger of the present invention, the extraction solvent is preferably a mixed solvent of water and a monohydric alcohol, more preferably a mixed solvent of water and ethyl alcohol. More preferably, it is a mixed solvent of water and ethyl alcohol having a mixing ratio of 50% by volume (hereinafter referred to as “vol%”) or more.

  The human lizard extract can be used as an extract, dried by spray drying or the like, or in a powder form, or a dry solid or powder mixed with an appropriate solvent according to the intended use. That is, it may be used as an active ingredient of a radical scavenger in any form such as liquid, paste or gel.

The human lysizuka extract, which is an active ingredient of the radical scavenger of the present invention, has an excellent scavenging ability with respect to radicals, particularly superoxide (O ₂ .−), that is, is particularly useful as a superoxide scavenger. . It is known that radicals such as superoxide are generated in the skin tissue by stimulation with ultraviolet rays or chemical substances, and as a result, oxidation proceeds to cause wrinkles and the like. Since the radical scavenger of the present invention has good safety to the skin, it is preferably blended in a skin external preparation and used to erase superoxide generated in the skin tissue. The skin external preparation containing the radical scavenger of the present invention exhibits an excellent antioxidant effect on the skin due to the excellent superoxide scavenging ability provided by the radical scavenger.
In addition, the radical scavenger of this invention can also be mix | blended with foodstuffs (a drink is included). Although it does not restrict | limit especially as said foodstuff, For example, a candy, chewing gum, a drink, etc. are mentioned.

Next, the skin external preparation of the present invention will be described in detail.
The skin external preparation of the present invention is an antioxidant skin external preparation containing, as an active ingredient, human lizards extract, which is the radical scavenger of the present invention. Although there is no restriction | limiting in particular about the compounding quantity of the said radical scavenger, The said human lizards extract is 0.00005-1.5 mass% (henceforth, only below) as solid content with respect to the total mass of the skin external preparation which is a final form. "%") Is preferable, and 0.0005 to 1.5% is more preferable. If it exists in this range, this component can be mix | blended stably, and high radical scavenging ability and the high antioxidant action based on it can be exhibited. In addition, when the extract is used, the concentration of the extract is not limited as long as the content of the human squirrel extract as a solute is within the above range.

  The form of the external preparation for skin according to the present invention is not particularly limited. Any form of cosmetic, such as a liniment, may be an external medicine.

  In addition to the radical scavenger, the skin external preparation of the present invention includes various components that are usually used in cosmetics, quasi-drugs, external medicines, ie, water, alcohols, oils, surfactants, Viscosity, powder, chelating agent, pH adjuster, UV absorber, extracts from animals and plants / microorganisms, various medicinal agents such as moisturizers, whitening agents, anti-inflammatory agents, cell activators, fragrances, etc. As long as the effect is not impaired, it can be added as appropriate.

  The present invention also relates to a method for scavenging radicals by supplying a human lizard extract. They may be supplied to a system in which radicals have already been generated to eliminate them, or may be supplied to a system in which radicals have not been generated to suppress their generation. For example, it can be applied to the surface of the skin to eliminate radicals generated in the skin tissue, particularly superoxide, or to suppress the generation of radicals, particularly superoxide, in the skin tissue. . It may be used for the purpose of improving the appearance of the skin, that is, beauty.

The present invention will be described more specifically with reference to the following examples. However, the scope of the present invention is not limited to the following examples.
[Example 1: Manufacture of 80 vol% hydrous ethyl alcohol extract of human lizards]
150 g of 80 vol% hydrated ethyl alcohol (the ratio of ethyl alcohol is 80 vol%) is added to 10 g of the whole plant of human squirrel and extracted at room temperature or heated for one day, followed by filtration, and the solvent in the obtained filtrate is filtered. Distilled to dryness, an 80 vol% water-containing ethyl alcohol extract (yield 0.8 g) of human lizards as a solid content was obtained.

[Example 2: Example of production of human lizardka 50 vol% hydrous ethyl alcohol extract]
150 g of 50 vol% hydrous ethyl alcohol (the ratio of ethyl alcohol is 50 vol%) is added to 10 g of human squirrel grass, extracted at room temperature or heated for one day, filtered, and the solvent in the obtained filtrate is filtered. Distilled to dryness to obtain a 50 vol% water-containing ethyl alcohol extract (yield: 0.75 g) of human lizards as a solid content.

[Example 3: Production example of purified water extract of Hitorizuka]
150 mL of purified water is added to 10 g of the whole plant of human lizard, and after extraction for one day at room temperature or warming, the mixture is filtered, and the solvent in the obtained filtrate is evaporated to dryness to obtain a solid content. A purified human water extract (yield 0.73 g) was obtained.

[Example 4: Evaluation of superoxide scavenging action]
In a test tube, 2.4 mL of 0.05 mol / L sodium bicarbonate buffer (pH 10.2), 0.1 mL of 3 mmol / L xanthine, 0.1 mL of 3 mmol / L EDTA, 50 μg / mL bovine 0.1 mL of serum albumin and 0.1 mL of 0.75 mmol / L nitroblue tetrazolium were added. To this, 0.1 mL of the test sample solution was added and left at 25 ° C. for 10 minutes. To a test tube, add 0.1 mL of an aqueous xanthine oxidase solution (an aqueous xanthine oxidase solution prepared so that the absorbance at a wavelength of 560 nm falls within the range of 0.2 to 0.23), stir rapidly, and react at 25 ° C. for 20 minutes. It was. Thereafter, 6 mmol / L copper chloride was added to each 0.1 mL to stop the reaction, and the absorbance at a wavelength of 560 nm was measured. A blank test was performed and corrected in the same manner.
The calculation method of the erasure rate is as follows.
Erase rate (%) = {1− (St−Sb) / (Ct−Cb)} × 100
St: Absorbance at wavelength 560 nm of test sample solution Sb: Absorbance at wavelength 560 nm of test sample solution blank Ct: Absorbance at wavelength 560 nm of control solution Cb: Absorbance at wavelength 560 nm of control solution blank An aqueous solution (concentration described in Table 1 below) was used.

As a comparative example, a similar test was also performed on an ougon extract that is already known to have a superoxide scavenging action.
In addition, 150 ml of 70 vol% hydrous ethyl alcohol (the ratio of ethyl alcohol is 70 vol%) was added to 10 g of whole grass of orgon, and extracted after 3 days at room temperature, and then filtered to remove the solvent. Then, 70 vol% water-containing ethyl alcohol extract of ougon obtained as a solid content by drying (yield 0.51 g). As a test sample, an aqueous solution of 70 vol% hydrous ethyl alcohol extract of ougon was used. Since the erasing ability was not observed at a concentration of 100 μg / mL or less, Table 1 below shows the results of using an aqueous solution of ougon extract having a concentration of 300 μg / mL as a test sample.
Table 1 below shows the calculated superoxide elimination rate of each sample and the concentration (IC ₅₀ (μg / mL)) at which each sample exhibits a superoxide elimination rate of 50%. The IC ₅₀ is a value calculated from a straight line obtained by plotting the obtained erasure rate value and drawing an approximate straight line. The same applies to IC _{50 in} Table 2.

[Example 5: Evaluation of DPPH radical scavenging action]
To a test tube, 3 mL of 150 μmol / L DPPH (diphenyl-p-picrylhydrazyl) ethyl alcohol solution and 3 mL of a test sample solution were added, each was tightly stoppered, and then shaken and allowed to stand for 30 minutes. After standing, the absorbance at a wavelength of 520 nm was measured. A blank test was performed and corrected in the same manner.
The calculation method of radical scavenging action on DPPH is as follows.
DPPH erasure rate (%) = {C− (St−Sb)} / C × 100
St: Absorbance at a wavelength of 520 nm of the test sample solution Sb: Absorbance at a wavelength of 520 nm of the test sample solution blank C: Absorbance at a wavelength of 520 nm of the control solution As each test sample, an ethyl alcohol solution of a human lysizuka extract (concentration is shown in Table 2 below) Used).

In addition, a similar test was performed on a chamomile extract that is already known to have a DPPH elimination action as a comparative example. The chamomile extract used was added to 100 g of chamomile whole plant with 100 mL of 70 vol% hydrous ethyl alcohol (the ratio of ethyl alcohol was 70 vol%), extracted at room temperature for 3 days, filtered, and the solvent was distilled off. It is a 70 vol% hydrous ethyl alcohol extract (yield 0.72 g) of chamomile obtained as a solid after leaving to dry. As a test sample, an aqueous solution of chamomile 70 vol% hydrous ethyl alcohol extract was used. Since no erasing ability was observed at a concentration of 200 μg / mL or less, Table 2 below shows the results for a test sample having a chamomile extract concentration of 300 μg / mL.
Table 2 below shows the calculated DPPH radical scavenging rate of each sample and the concentration (IC ₅₀ (μg / mL)) at which each sample exhibits a DPPH radical scavenging rate of 50%.

[Example 6: Cream]
Creams having the compositions shown in Table 3 below were prepared by the following methods.
First, components (1) to (6) and (9) shown in Table 3 were mixed, heated and maintained at 70 ° C. A part of component (11) heated to 70 ° C. was added to this mixture, emulsified, and (7) to (8) and (10) dissolved in the remainder of (11) were further mixed. . Then, it cooled and each cream was obtained.

(Test method)
A panel of 15 females aged 35 to 59 years old per test cream, and an appropriate amount of the test cream was applied to the face after washing the face twice a morning and night for 12 weeks. The wrinkle improvement effect by application was evaluated according to the following criteria, and the number of people corresponding to each evaluation criterion is shown in Table 3.
(Evaluation criteria)
<Evaluation><Contents>
Effective Wrinkles on the skin are not noticeable.
Slightly effective Skin wrinkles are less noticeable.
Invalid No change before use.

(Composition and results)

  From the results shown in Table 3, the cream of the product 1 of the present invention formulated with 80 vol% water-containing ethyl alcohol extract of human lizards can improve the wrinkle of the skin by applying these to the skin, and it is beautiful with tension. It became clear that it was skin.

Hereinafter, examples of blending into cosmetics will be shown, but not limited to these prescriptions.
[Example 7: Cleansing cream]
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 3.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Beeswax 1.5
(5) Vaseline 6.0
(6) Liquid paraffin 40.0
(7) Dimethylpolysiloxane (100CS) 0.5
(8) Sorbitan sesquioleate 1.0
(9) Preservative appropriate amount (10) Triethanolamine 1.0
(11) Propylene glycol 10.0
(12) Polyethylene glycol 20000 0.5
(13) Carboxyvinyl polymer 0.05
(14) Purified water Residual amount (15) Human vol. 50 vol% hydrous ethyl alcohol extract * 1 0.5
(16) Soybean extract * 2 0.5
(17) Mallow extract * 3 0.5
(18) Fragrance Appropriate amount * 1: What was obtained by dissolving the extract produced in Example 2 in 50 vol% hydrous 1,3-butylene glycol to a content of 1% * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. Made (Manufacturing method)
A. Ingredients (1) to (9) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (10) to (14) are dissolved by heating and maintained at 70 ° C.
C. A is added to B and emulsified.
D. After cooling C, components (15) to (18) were added and mixed to obtain a cleansing cream.
The obtained cleansing cream was a gentle cleansing cream that spreads lightly and has good makeup removal, prevents oxidation of skin cell tissues, and maintains healthy skin.

[Example 8: Face wash]
(Ingredient) (%)
(1) Lauric acid 5.0
(2) Myristic acid 18.5
(3) Stearic acid 6.0
(4) Glycerin 12.0
(5) Polyethylene glycol 1500 5.0
(6) Potassium hydroxide 6.5
(7) Purified water remaining amount (8) Palm oil fatty acid diethanolamide 5.0
(9) Palm oil fatty acid methyl taurine sodium 1.8
(10) Polyoxyethylene (7.5E.O.) lauryl ether 2.0
(11) Ethylene glycol distearate 1.0
(12) Hydroxypropyl methylcellulose 1% aqueous solution 5.0
(13) Human squirrel 80 vol% hydrous ethyl alcohol extract * 1 1.0
(14) Maronnier extract * 2 1.0
(15) Tea extract * 3 0.5
(16) Fragrance Appropriate amount * 1: What was obtained by dissolving the extract produced in Example 1 in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 2: Koei Kogyo Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. Company (production method)
A. Components (1) to (7) are dissolved by heating.
B. Components (8) to (11) are dissolved by heating.
C. Add B to A and mix.
D. After cooling C, components (12) to (16) are added and mixed to obtain a face wash.
The obtained cleansing cream and facial cleanser had a fine and rich foaming and a refreshing feeling of use, and was a facial cleanser that prevented oxidation of skin cell tissues and maintained healthy skin.

[Example 9: Lotion 1]
(Ingredient) (%)
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution * 1 0.5
(4) Glycerin 3.0
(5) 1,3-butylene glycol 8.0
(6) L-ascorbic acid 2-glucoside * 2 2.0
(7) Sodium hydroxide 0.25
(8) Hiritishika purified water extract * 3 1.0
(9) Yokuinin extract * 4 1.0
(10) Rosemary extract * 5 1.0
(11) Purified water remaining amount (12) Ethyl alcohol 10.0
(13) Perfume Appropriate amount (14) Preservative Appropriate amount (15) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1: Ajinomoto Co., Inc. * 2: Hayashibara Biochemical Laboratories Co., Ltd. * 3: The extract produced in Example 3 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 4 : Made by Maruzen Pharmaceutical * 5: Made by Maruzen Pharmaceutical (Manufacturing method)
A. Components (1) to (11) are mixed and dissolved.
B. Components (12) to (15) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.
The obtained lotion 1 had a fresh and refreshing feeling of use, was a lotion that prevented the oxidation of skin cell tissues, kept the skin fresh and smoothed the skin.

[Example 10: Lotion 2]
(Ingredient) (%)
(1) Meadow home oil 0.1
(2) Jojoba oil 0.05
(3) Perfume appropriate amount (4) preservative appropriate amount (5) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(6) Isostearic acid polyoxyethylene hydrogenated castor oil (50 EO) 1.0
(7) Ethyl alcohol 8.0
(8) Glycerin 5.0
(9) 1,3-butylene glycol 5.0
(10) Polyethylene glycol 1500 0.1
(11) Hitorishizuka 80vol% hydrous ethyl alcohol extract * 1 1.0
(12) Dipotassium glycyrrhizinate * 2 0.5
(13) Licorice extract * 3 0.5
(14) Remaining amount of purified water * 1: The extract produced in Example 1 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Made by Pharmaceutical Company (Manufacturing method)
A. Components (1) to (7) are mixed and dissolved.
B. Components (8) to (14) are mixed and dissolved.
C. A is added to B and mixed to obtain a skin lotion.
The obtained lotion 2 had a fresh and mellow feeling of use, was a lotion that prevented the oxidation of skin cell tissues, kept the skin fresh and smoothed the skin.

[Example 11: Lotion 3]
(Ingredient) (%)
(1) Glyceryl tri-2-ethylhexanoate 0.08
(2) Squalane 0.02
(3) Sorbitan sesquioleate 0.05
(4) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.05
(5) Polyoxyethylene (8E.O.) alkylene (12-15)
Ether phosphoric acid 0.1
(6) Preservative appropriate amount (7) perfume appropriate amount (8) ethyl alcohol 8.0
(9) Dipropylene glycol 8.0
(10) Glycerin 4.0
(11) Hitorishizuka 50 vol% hydrous ethyl alcohol extract * 1 0.5
(12) Serine * 2 0.1
(13) Acetylglutamic acid * 3 0.1
(14) Purified water remaining amount * 1: The extract produced in Example 2 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 2: Ajinomoto Co. * 3: Kyowa Hakko Company (production method)
A. Components (1) to (8) are mixed and dissolved.
B. Components (9) to (14) are mixed and dissolved.
C. A is added to B and emulsified to obtain a skin lotion.
The obtained lotion 3 had a clean and light feeling of use, was a lotion that prevented the oxidation of skin cell tissues, kept the skin fresh and smoothed the skin.

[Example 12: Latex]
(Ingredient) (%)
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Preservative appropriate amount (9) Sorbitan monostearate 0.3
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(11) Stearyl glycyrrhetinate * 1 0.2
(12) Triethanolamine 0.5
(13) 1,3-butylene glycol 15.0
(14) Glycerin 3.0
(15) Polyethylene glycol 6000 0.5
(16) Purified water remaining amount (17) 1% solution of carboxyvinyl polymer 8.0
(18) Human squirrel 50 vol% hydrous ethyl alcohol extract * 2 0.5
(19) Warmoko extract * 3 0.5
(20) Onionis extract * 4 0.5
(21) Fragrance Appropriate amount * 1: Made by Maruzen Pharmaceutical Co., Ltd. * 2: The extract produced in Example 2 dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 3: Maruzen Pharmaceutical Co., Ltd. * 4: manufactured by Maruzen Pharmaceutical Co., Ltd.
A. Components (1) to (11) are dissolved by heating and maintained at 70 ° C.
B. Components (12) to (16) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and component (17) is further added and mixed.
D. C is cooled, and components (18) to (21) are added and mixed to obtain an emulsion.
The obtained emulsion had a smooth and mellow feeling of use, prevented the oxidation of skin cell tissues, imparted a moisturizing feeling and an appropriate emollient feeling to the skin, and made the skin soft.

[Example 13: Cream]
(Ingredient) (%)
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester * 1 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Preservative appropriate amount (14) Triethanolamine 1.2
(15) 1,3-butylene glycol 8.0
(16) Glycerin 2.0
(17) Polyethylene glycol 20000 0.5
(18) Purified water Remaining amount (19) Carboxyvinyl polymer 1% aqueous solution 10.0
(20) Arbutin * 2 3.0
(21) Human squirrel 80 vol% hydrous ethyl alcohol extract * 3 1.0
(22) Sodium hyaluronate 1% aqueous solution * 4 5.0
(23) Licorice extract * 5 0.5
(24) Peach extract * 6 0.5
(25) Perfume appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2: Wako Pure Chemical Industries, Ltd. * 3: The extract produced in Example 1 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 4: Kibun Food Chemifa Corporation * 5: Made by Maruzen Pharmaceutical * 6: Made by Maruzen Pharmaceutical (Production method)
A. Ingredients (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (14) to (18) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and component (19) is further added and mixed.
D. C is cooled, components (20) to (25) are added and mixed to obtain a cream.
The obtained cream had a smooth and rich feeling of use, prevented oxidation of skin cell tissue, imparted a high emollient feeling to the skin, and made the skin soft.

[Example 14: Cosmetic liquid]
(Ingredient) (%)
(1) Glyceryl tri-2-ethylhexanoate 0.1
(2) Meadow Home Oil 0.05
(3) Jojoba oil 0.05
(4) Retinol palmitate * 1 0.2
(5) Tocopherol acetate * 2 0.1
(6) Preservative appropriate amount (7) perfume appropriate amount (8) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(9) Polyoxyethylene hydrogenated isostearate castor oil (50 EO) 1.5
(10) Ethyl alcohol 5.0
(11) Glycerin 4.0
(12) Dipropylene glycol 8.0
(13) 1,3-butylene glycol 8.0
(14) Sodium lactate 0.5
(15) Sodium pyrrolidonecarboxylate (50%) solution * 3 0.5
(16) Hydroxyethyl cellulose 0.08
(17) Sodium alginate 0.05
(18) Human squirrel 50 vol% hydrous ethyl alcohol extract * 4 0.2
(19) Hermanus extract * 5 0.5
(20) Peonies extract * 6 0.5
(21) Remaining amount of purified water * 1: Roche * 2: Eisai * 3: Ajinomoto * 4: 50 vol% water containing 1,3 so that the extract produced in Example 2 has a content of 1% -Dissolved in butylene glycol * 5: manufactured by Maruzen Pharmaceutical Co., Ltd. * 6: manufactured by Maruzen Pharmaceutical Co., Ltd. (production method)
A. Components (1) to (9) are mixed and dissolved.
B. Components (10) to (21) are mixed and dissolved.
C. A is added to B and mixed to obtain a serum.
The essence obtained was a essence that had a mellow and mild feeling of use, prevented the oxidation of skin cell tissues, gave the skin a high moisturizing and emollient feeling, and made the skin fresh and soft. .

[Example 15: Pack (Peel-off type)]
(Ingredient) (%)
(1) Polyvinyl alcohol 12.0
(2) Methylcellulose 0.1
(3) Glycerin 3.0
(4) 1,3-butylene glycol 5.0
(5) Purified water remaining amount (6) perfume appropriate amount (7) preservative appropriate amount (8) glyceryl tri-2-ethylhexanoate 0.1
(9) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.0
(10) Ethyl alcohol 13.0
(11) Hitori Shizuka 80vol% hydrous ethyl alcohol extract * 1 0.5
(12) Asparagus extract * 2 0.5
(13) Chamomile extract * 3 0.5
* 1: The extract produced in Example 1 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. (manufacturing method)
A. Components (1) to (5) are dissolved by heating.
B. Components (6) to (10) are mixed and dissolved.
C. After cooling A, B and components (11) to (13) are added and mixed to obtain a pack.
The resulting pack has a moderate refreshing feeling and high tension, prevents oxidation of skin cell tissues, imparts an appropriate moisturizing feeling and elasticity to the skin after packing, and softens the skin. It was a pack.

[Example 16: Massage cream]
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Sorbitan sesquioleate 1.0
(5) Cetyl palmitate 1.0
(6) Dipentaerythritol fatty acid ester * 1 4.0
(7) Vaseline 20.0
(8) Liquid paraffin 28.0
(9) Dimethylpolysiloxane (100CS) 0.5
(10) Sodium hydroxide 0.1
(11) Dipropylene glycol 7.0
(12) Carboxyvinyl polymer 0.1
(13) Purified water Residual amount (14) Human volcano 80vol% hydrous ethyl alcohol extract * 2 1.0
(15) Hydrolyzed conchiolin * 3 0.1
(16) Carrot extract * 4 1.0
(17) Perfume appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2: The extract produced in Example 1 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 3: manufactured by Maruzen Pharmaceutical Co., Ltd. * 4: manufactured by Maruzen Pharmaceutical Co., Ltd. (manufacturing method)
A. Ingredients (1) to (9) are dissolved by heating and maintained at 70 ° C.
B. Components (10) to (13) are dissolved by heating and maintained at 70 ° C.
C. A is added to B and emulsified.
D. After cooling C, components (14) to (17) were added and mixed to obtain a massage cream.
The resulting massage cream has a rich and smooth feeling of use, has a high massage effect, prevents oxidation of the skin cell tissue, moisturizes the skin, and gives the skin a smooth feel. Met.

[Example 17: Liquid foundation]
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Cetanol 0.5
(3) Behenyl alcohol 1.0
(4) Petrolatum 2.5
(5) Liquid paraffin 5.0
(6) Self-emulsifying glyceryl monostearate 1.0
(7) Preservative appropriate amount (8) Titanium oxide 6.0
(9) Color pigment 4.0
(10) Mica 2.0
(11) Talc 4.0
(12) Carboxymethylcellulose 0.2
(13) Bentonite 0.4
(14) Purified water Residual amount (15) Purified water extract * 1 0.5
(16) Peppermint extract * 2 0.5
(17) Shobu extract * 3 0.5
(18) Fragrance Appropriate amount * 1: The extract prepared in Example 3 dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. Made (Manufacturing method)
A. Components (1) to (7) are dissolved by heating.
B. Ingredients (8) to (11) are added to A, mixed uniformly, and kept at 70 ° C.
C. Ingredients (12) to (14) are dissolved by heating and maintained at 70 ° C.
D. B is added to C and emulsified.
E. After cooling D, components (15) to (18) were added and mixed to obtain a liquid foundation.
The obtained liquid foundation was a liquid foundation that had a light and expansive feeling of use, prevented oxidation of the skin cell tissue, and had a uniform and beautiful finish.

  The radical scavenger of the present invention can be used as an active ingredient for external preparations for skin, foodstuffs and the like. In particular, it is useful for external preparations for skin such as cosmetics.

Claims (5)

A radical scavenger containing as an active ingredient an extract of human lizard water or a mixed solvent of water and ethyl alcohol. The radical scavenger according to claim 1, which has an scavenging action on at least superoxide. Antioxidants which comprises, as an active ingredient an extract with a mixed solvent of water or water and ethyl alcohol Hitorishizuka. By feeding extracts to the skin by water or a mixed solvent of water and ethyl alcohol Hitorishizuka, how to erase the radicals (except human therapeutic methods). The method according to claim 4, wherein at least superoxide is eliminated.