CN111567350A - Planting method for promoting nitrogen fixation of sugarcane - Google Patents

Planting method for promoting nitrogen fixation of sugarcane Download PDF

Info

Publication number
CN111567350A
CN111567350A CN202010362625.8A CN202010362625A CN111567350A CN 111567350 A CN111567350 A CN 111567350A CN 202010362625 A CN202010362625 A CN 202010362625A CN 111567350 A CN111567350 A CN 111567350A
Authority
CN
China
Prior art keywords
sugarcane
parts
nitrogen
fertilizer
fixing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202010362625.8A
Other languages
Chinese (zh)
Other versions
CN111567350B (en
Inventor
罗霆
李长宁
林丽
谢金兰
莫璋红
李杨瑞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Original Assignee
Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences filed Critical Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority to CN202010362625.8A priority Critical patent/CN111567350B/en
Publication of CN111567350A publication Critical patent/CN111567350A/en
Application granted granted Critical
Publication of CN111567350B publication Critical patent/CN111567350B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/55—Sugar cane
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/10—Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12—Granules or flakes
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/30—Layered or coated, e.g. dust-preventing coatings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Ecology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to the technical field of sugarcane planting, and particularly discloses a planting method for promoting nitrogen fixation of sugarcane, which comprises the following steps: carrying out detoxification treatment on the single-bud seed stems of the sugarcane; carrying out sand culture on the single-bud seed stems of the sugarcane, and carrying out nitrogen-fixing bacterium inoculation treatment on the sugarcane seedlings when the sugarcane seedlings grow 3-4 leaves; scientifically applying fertilizer in the soil preparation ditch in combination with the cooperation of a nitrogen-phosphorus-potassium base fertilizer, a nitrogen-fixing microbial fertilizer and an organic fertilizer to provide nutrition required by the growth of the sugarcane, and planting the inoculated sugarcane seedlings; and after the sugarcane seedlings grow for 2-3 months, spraying a nitrogen fixation synergist solution on the sugarcane leaf surfaces. The planting method provided by the invention improves the nitrogen fixation capacity of the sugarcane, reduces the application times and the usage amount of the nitrogen fertilizer, improves the yield and the quality of the sugarcane, and improves the economic benefit of the sugarcane.

Description

Planting method for promoting nitrogen fixation of sugarcane
Technical Field
The invention belongs to the technical field of sugarcane planting, and particularly relates to a planting method for promoting sugarcane nitrogen fixation.
Background
Sugarcane is a general term of the genus Saccharum and is native to tropical and subtropical regions. Sugarcane is an important sugar-producing crop, and the planting area of the sugarcane is especially large in Guangxi areas of China. The planted sugarcane has high light energy utilization rate and land productivity per unit area, and is a crop with larger economic benefit compared with the planted grain crop. Nitrogen is one of three essential elements for the growth of sugarcane, is a component of important life substances such as protein, chlorophyll, nucleic acid, enzyme, biological hormone and the like, and is a main nutrient element influencing the yield and quality of the sugarcane. Guangxi is the leading sugarcane production area in China, measures are adopted to improve the sugarcane yield, nitrogen fertilizer can be applied for multiple times in the sugarcane growth period, and the nitrogen fertilizer application amount in sugarcane areas in China is generally 500-700 kg/hm2Under the condition of equivalent yield, the nitrogen fertilizer using amount in China is 8-10 times of that in Brazil, the nitrogen fertilizer using rate in China is low, and a large amount of nitrogen fertilizer is input, so that the production cost of sugarcane is increased, and the economic benefit of sugarcane is reduced; in addition, when a large amount of nitrogen fertilizer is applied, the nitrification and denitrification of the soil reduce the fertility of the soil and cause serious pollution to the ecological environment. If the absorption efficiency of the sugarcane to nitrogen fertilizer and the nitrogen fixation capacity of the sugarcane can be improved, the use amount of the nitrogen fertilizer is favorably reduced, the production investment of the sugarcane is reduced, the pollution to the environment can be reduced, and the yield of the sugarcane and the quality of the sugarcane are improved.
Disclosure of Invention
The invention aims to provide a planting method for promoting nitrogen fixation of sugarcane, which enhances the nitrogen fixation capacity of the sugarcane, thereby improving the utilization rate of nitrogen fertilizer, reducing the use amount of the nitrogen fertilizer and improving the yield and quality of the sugarcane.
In order to achieve the aim, the invention provides a planting method for promoting nitrogen fixation of sugarcane, which comprises the following steps:
(1) soaking the sugarcane single-bud seed stems in water at 50 ℃ for 1-2 h, and then soaking in 800 times of 50% carbendazim solution for 5-10 min to obtain detoxified sugarcane single-bud seed stems;
(2) carrying out sand culture on the detoxified sugarcane single-bud seed stems, removing sugarcane seedlings from sand when 3-4 leaves grow out of the sugarcane, cutting off small root parts of the sugarcane seedlings, and soaking the root parts of the sugarcane seedlings in azotobacter liquid for 30-40 min to obtain inoculated sugarcane seedlings;
(3) soil preparation ditching is carried out to the soil of farming, and the science is fertilized in the ditch, the fertilizer contains the three-layer, from the top down is basic fertilizer layer, nitrogen fixation microbial fertilizer layer and organic fertilizer layer in proper order, basic fertilizer layer is nitrogen phosphorus potassium fertilizer layer, and the rate of fertilizer application: the base fertilizer layer is 280-350 kg/hm2The nitrogen-fixing microbial fertilizer layer is 110-150 kg/hm2The organic fertilizer layer is 180-220 kg/hm2Spreading fine sand on the surface of the base fertilizer layer, planting sugarcane seedlings subjected to inoculation, wherein the row spacing of the planted sugarcanes is 90-100 cm, and the spacing between the sugarcane seedlings is 20-30 cm;
(4) after sugarcane seedlings are planted for 2-3 months, spraying a nitrogen fixation synergist solution on sugarcane leaf surfaces, wherein the spraying amount is 50-65L/mu, and the nitrogen fixation synergist comprises the following components in percentage by weight: 1-5% of seaweed extract, 1-2% of fulvic acid, 1-2% of rhamnolipid, 0.05-0.1% of molybdic acid, 0.05-0.08% of ferric sulfite and the balance of water; the nitrogen fixation synergist solution is prepared by diluting a nitrogen fixation synergist with water to form a solution with the concentration of 400-500 ppm.
Preferably, in the planting method, in the step (2), the nitrogen-fixing bacteria liquid is a nitrogen-fixing snail bacteria liquid, and the concentration of the nitrogen-fixing snail bacteria in the nitrogen-fixing snail bacteria liquid is 105～106CFU/ml。
Preferably, in the planting method, the preparation method of the azospira liquid comprises the following steps:
(I) inoculating azospirillum into a liquid culture medium, and culturing for 24-48 h at the temperature of 28-30 ℃ and the speed of 180-200 r/min, wherein the liquid culture medium comprises the following components in parts by weight: 10 parts of cane sugar, 0.1 part of dipotassium phosphate, 0.2 part of magnesium sulfate heptahydrate, 0.02 part of calcium chloride, 0.002 part of sodium molybdate, 5.0 parts of malic acid, 0.4 part of monopotassium phosphate, 0.1 part of sodium chloride, 0.01 part of ferric chloride and 1000 parts of water; mixing the components, adjusting the pH to 7.0-7.2, then carrying out high-pressure sterilization at 121 ℃ for 30min, and cooling to obtain a liquid culture medium;
(II) centrifuging the liquid culture medium subjected to the inoculation culture in the step (I) to collect thalli, wherein the centrifugation conditions are 4000-6000 r/min and 3-6 min, suspending the thalli by using a sterile 3% sucrose solution, and diluting until the concentration of the thalli is 105～106CFU/ml to obtain the azospirillum liquid.
Preferably, in the planting method, the base fertilizer of the base fertilizer layer is obtained by mixing the following components in parts by weight: 20-35 parts of urea, 10-15 parts of phosphorus pentoxide, 10-18 parts of potassium nitrate and 20-35 parts of decomposed straw.
Preferably, in the planting method, the nitrogen-fixing microbial fertilizer of the nitrogen-fixing microbial fertilizer layer is prepared from the following raw materials in parts by weight: 40-50 parts of bagasse, 30-40 parts of vinasse, 5-10 parts of molasses, 1-2 parts of saccharomycetes, 1-1.5 parts of nitrogen-fixing blue algae, 2-2.5 parts of azotobacter chroococcum, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus mucilaginosus;
the nitrogen-fixing microbial fertilizer is prepared by the following method: uniformly mixing bagasse, vinasse and molasses to obtain a mixture, controlling the water content of the mixture to be 60-70%, adding activated yeast, performing primary fermentation at 50-60 ℃ for 2-3 days, and cooling to room temperature; and then adding activated azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus, uniformly mixing, and performing secondary fermentation at 40-50 ℃ for 2-3 days to obtain the nitrogen-fixing microbial fertilizer.
Preferably, in the planting method, the organic fertilizer of the organic fertilizer layer comprises the following raw materials in parts by weight: 80-90 parts of chicken manure, 20-40 parts of wheat bran, 10-15 parts of humic acid, 0.5-1 part of lactic acid bacteria, 0.1-0.3 part of Aspergillus niger, 6-12 parts of zeolite powder, 6-12 parts of bentonite and 6-12 parts of hydroxypropyl-beta-cyclodextrin.
Preferably, in the planting method, the preparation method of the organic fertilizer comprises the following steps:
uniformly mixing chicken manure, wheat bran, humic acid and zeolite powder to obtain a mixture, controlling the water content of the mixture to be 50-60%, adding activated lactic acid bacteria and aspergillus niger, and carrying out aerobic fermentation for 4-5 days at 35-45 ℃;
drying and granulating the fermented material to obtain fertilizer granules, wherein the grain size of the granules is 2-3 mm;
dissolving hydroxypropyl-beta-cyclodextrin by using water to obtain a cyclodextrin solution, wherein the mass of the water is 2-3 times that of the hydroxypropyl-beta-cyclodextrin, adding bentonite into the cyclodextrin solution, and uniformly stirring and mixing to obtain slurry;
and fourthly, adding the fertilizer particles obtained in the third step into a coating machine, spraying the slurry obtained in the third step under the rotation of the coating machine, spraying the slurry on the surfaces of the fertilizer particles, and drying to form coatings on the surfaces of the fertilizer particles to obtain the organic fertilizer.
Preferably, in the planting method, the nitrogen fixation synergist comprises the following components in percentage by weight: 3-5% of seaweed extract, 1.5-2% of fulvic acid, 1-1.5% of rhamnolipid, 0.05-0.08% of molybdic acid, 0.05-0.06% of ferric sulfite and the balance of water.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the planting method for promoting the nitrogen fixation of the sugarcane, the nitrogen fixation capability of the sugarcane is improved, the application times and the usage amount of nitrogen fertilizer are reduced, the yield and the quality of the sugarcane are improved, and the economic benefit of the sugarcane is improved by inoculating nitrogen-fixing bacteria to sugarcane seedlings and spraying nitrogen-fixing synergist solution to sugarcane leaves.
2. According to the planting method, a scientific fertilizing mode is adopted, and the nitrogen-phosphorus-potassium base fertilizer, the nitrogen-fixing microbial fertilizer and the organic fertilizer are applied in a matched mode, so that nutrition required by sugarcane growth is provided, the sugarcane growth is promoted, the fertilizer utilization rate is improved, the application amount of the chemical fertilizer can be effectively reduced, and the soil can be improved.
3. According to the planting method, the nitrogen fixation synergist solution is sprayed, rhamnolipid in the nitrogen fixation synergist solution can reduce the surface tension of plant leaves, so that the nitrogen fixation synergist solution is better attached to the leaf surfaces and fully spread on the leaf surfaces, and the nitrogen fixation synergist solution is promoted to be fully absorbed; the absorbed seaweed extract, fulvic acid and the like can synergistically enhance the nitrogen-fixing enzyme activity of azotobacter in the sugarcane body, promote the absorption and utilization of the sugarcane on nitrogen and improve the nitrogen-fixing capacity of the sugarcane.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
A planting method for promoting nitrogen fixation of sugarcane is characterized by comprising the following steps:
(1) soaking the single-bud seed stems of the sugarcane in water at 50 ℃ for 1h, and then soaking in 800 times of 50% carbendazim solution for 5min to obtain detoxified single-bud seed stems of the sugarcane;
(2) carrying out sand culture on the detoxified sugarcane single-bud seed stems, removing sugarcane seedlings from sand when 3-4 leaves grow out of the sugarcane, cutting off small root parts of the sugarcane seedlings, soaking the root parts of the sugarcane seedlings in azospirillum solution for 30min, wherein the concentration of azospirillum in the azospirillum solution is 106CFU/ml to obtain inoculated sugarcane seedlings;
(3) soil preparation and ditching are carried out on the cultivated land, fertilizer is scientifically applied in the ditch, the fertilizer comprises three layers, namely a base fertilizer layer, a nitrogen-fixing microbial fertilizer layer and an organic fertilizer layer from top to bottom, and the fertilizing amount is as follows: the base fertilizer layer is 280kg/hm2The nitrogen-fixing microbial fertilizer layer is 120kg/hm2The organic fertilizer layer is 200kg/hm2Spreading fine sand on the surface of the base fertilizer layer, planting sugarcane seedlings subjected to inoculation, wherein the row spacing of the planted sugarcanes is 90-100 cm, and the spacing between the sugarcane seedlings is 20-30 cm;
(4) after 3 months of planting sugarcane seedlings, spraying a nitrogen fixation synergist solution on the sugarcane leaf surfaces, wherein the spraying amount is 60L/mu, and the nitrogen fixation synergist comprises the following components in percentage by weight: 4% of seaweed extract, 1.5% of fulvic acid, 1.5% of rhamnolipid, 0.08% of molybdic acid, 0.05% of ferric sulfite and the balance of water; diluting the nitrogen fixation synergist into a solution with the concentration of 450ppm by using water to obtain the nitrogen fixation synergist solution.
In the step (2), the preparation method of the azospira liquid comprises the following steps:
(I) inoculating azospirillum into a liquid culture medium, and culturing for 24h at 30 ℃ and 200r/min, wherein the liquid culture medium comprises the following components in parts by weight: 10 parts of cane sugar, 0.1 part of dipotassium phosphate, 0.2 part of magnesium sulfate heptahydrate, 0.02 part of calcium chloride, 0.002 part of sodium molybdate, 5.0 parts of malic acid, 0.4 part of monopotassium phosphate, 0.1 part of sodium chloride, 0.01 part of ferric chloride and 1000 parts of water; mixing the components, adjusting the pH to 7.0-7.2, then carrying out high-pressure sterilization at 121 ℃ for 30min, and cooling to obtain a liquid culture medium;
(II) centrifuging the liquid culture medium after the inoculation culture in the step (I) to collect thalli, wherein the centrifugation conditions are 5000r/min and 5min, suspending the thalli by using a sterile 3% sucrose solution, and diluting until the concentration of the thalli is 106CFU/ml to obtain the azospirillum liquid.
The base fertilizer of the base fertilizer layer is prepared by mixing the following components in parts by weight: 30 parts of urea, 15 parts of phosphorus pentoxide, 15 parts of potassium nitrate and 28 parts of decomposed straw.
The nitrogen-fixing microbial fertilizer of the nitrogen-fixing microbial fertilizer layer is composed of the following raw materials in parts by weight: 45 parts of bagasse, 34 parts of vinasse, 8 parts of molasses, 1 part of yeast, 1.2 parts of nitrogen-fixing blue algae, 2.5 parts of azotobacter chroococcum, 1.5 parts of bacillus megaterium and 1.5 parts of bacillus mucilaginosus. The nitrogen-fixing microbial fertilizer is prepared by the following method: mixing bagasse, vinasse and molasses together to obtain a mixture, controlling the water content of the mixture to be 60-70%, adding activated yeast, performing primary fermentation at 55 ℃ for 2 days, and cooling to room temperature; then adding activated azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus, uniformly mixing, and carrying out secondary fermentation for 3 days at the temperature of 45 ℃ to obtain the nitrogen-fixing microbial fertilizer.
The organic fertilizer of the organic fertilizer layer comprises the following raw materials in parts by weight: 90 parts of chicken manure, 35 parts of wheat bran, 12 parts of humic acid, 0.8 part of lactic acid bacteria, 0.2 part of aspergillus niger, 10 parts of zeolite powder, 8 parts of bentonite and 8 parts of hydroxypropyl-beta-cyclodextrin. The preparation method of the organic fertilizer layer fertilizer comprises the following steps:
uniformly mixing chicken manure, wheat bran, humic acid and zeolite powder to obtain a mixture, controlling the water content of the mixture to be 50-60%, adding activated lactic acid bacteria and aspergillus niger, and carrying out aerobic fermentation for 4 days at 40 ℃;
drying and granulating the fermented material to obtain fertilizer granules, wherein the grain size of the granules is 2-3 mm;
dissolving hydroxypropyl-beta-cyclodextrin by using water to obtain a cyclodextrin solution, wherein the mass of the water is 2 times that of the hydroxypropyl-beta-cyclodextrin; adding the cyclodextrin solution into bentonite, and stirring and mixing uniformly to obtain slurry;
and fourthly, adding the fertilizer particles obtained in the third step into a coating machine, spraying the slurry obtained in the third step under the rotation of the coating machine, spraying the slurry on the surfaces of the fertilizer particles, and drying to form coatings on the surfaces of the fertilizer particles to obtain the organic fertilizer.
In the nitrogen fixation synergist, the seaweed extract contains abundant carbohydrates (xylan, anhydroglycan and the like), nitrogen-containing compounds (amino acids, peptides, amines and the like), auxin-indole compounds, betaine and the like, and can strengthen the activity of nitrogen fixation enzyme in sugarcane and regulate the growth and development of the sugarcane. In addition, the seaweed extract contains alginic acid, which can reduce the surface tension of water, form a layer of film on the surface of the plant leaf, increase the contact area, and make water or water-soluble substances easily permeate the cell membrane on the leaf surface to enter the plant cell.
The fulvic acid has small molecular weight, is easy to be absorbed by the sugarcane, has high functional group content, can enhance the activity of various enzymes and the content of chlorophyll in the sugarcane, enables the metabolism to be vigorous, enhances the photosynthesis, increases the sugar content and dry matter of the sugarcane, and can enhance the stress resistance of the sugarcane.
The rhamnolipid is a biological surfactant, can reduce the surface tension of plant leaves, so that the sprayed nitrogen fixation synergist solution is better attached to the leaf surfaces and fully spread on the leaf surfaces, and the nitrogen fixation synergist solution is promoted to be fully absorbed; in addition, the rhamnolipid has the functions of killing insects, inhibiting bacteria and preventing diseases.
Molybdic acid and ferric sulfite respectively provide molybdenum element and iron element for sugarcane, the azotase is formed by compounding ferromolybdenum protein and ferritin, and sufficient iron element and molybdenum element can ensure azotase efficiency and remarkably improve azotase activity.
The base fertilizer layer provides necessary nutrients for the initial growth of the sugarcane and promotes the growth of root systems. The nitrogen-fixing microbial fertilizer layer provides sugar and nutrients for the growth of the sugarcane, so that the sugarcane produced in a large scale has high sweetness and enough moisture, the stress resistance of the sugarcane is enhanced, the micro-ecological environment of the plant rhizosphere soil is activated, nitrogen is fixed, insoluble nutrient elements such as potassium, phosphorus, magnesium and the like are promoted to be converted into soluble nutrients, the fertilizer absorption level and the fertilizer utilization rate of the sugarcane are improved, and the using amount of a chemical fertilizer is reduced. The organic fertilizer layer is organic matter and rich amino acid required by the sugarcane in the later period, and the surface of the organic fertilizer layer fertilizer particles is coated with the coating film, so that the slow release effect is achieved, the loss of fertilizer nutrients is prevented, and the fertilizer keeps the fertility.
Example 2
A planting method for promoting nitrogen fixation of sugarcane is characterized by comprising the following steps:
(1) soaking the single-bud seed stems of the sugarcane in water at 50 ℃ for 1h, and then soaking in 800 times of 50% carbendazim solution for 8min to obtain detoxified single-bud seed stems of the sugarcane;
(2) carrying out sand culture on the detoxified sugarcane single-bud seed stems, removing sugarcane seedlings from sand when 3-4 leaves grow out of the sugarcane, cutting off small root parts of the sugarcane seedlings, soaking the root parts of the sugarcane seedlings in azospirillum solution for 30min, wherein the concentration of azospirillum in the azospirillum solution is 105CFU/ml to obtain inoculated sugarcane seedlings;
(3) soil preparation and ditching are carried out on the cultivated land, fertilizer is scientifically applied in the ditch, the fertilizer comprises three layers, namely a base fertilizer layer, a nitrogen-fixing microbial fertilizer layer and an organic fertilizer layer from top to bottom, and the fertilizing amount is as follows: the base fertilizer layer is 320kg/hm2The nitrogen-fixing microbial fertilizer layer is 150kg/hm2The organic fertilizer layer is 180kg/hm2Spreading fine sand on the surface of the base fertilizer layer, planting sugarcane seedlings subjected to inoculation, wherein the row spacing of the planted sugarcanes is 90-100 cm, and the spacing between the sugarcane seedlings is 20-30 cm;
(4) after 2 months of planting sugarcane seedlings, spraying a nitrogen fixation synergist solution on the sugarcane leaf surfaces, wherein the spraying amount is 60L/mu, and the nitrogen fixation synergist comprises the following components in percentage by weight: 5% of seaweed extract, 2% of fulvic acid, 1.2% of rhamnolipid, 0.06% of molybdic acid, 0.06% of ferric sulfite and the balance of water; diluting the nitrogen fixation synergist into a solution with the concentration of 450ppm by using water to obtain the nitrogen fixation synergist solution.
In the step (2), the preparation method of the azospira liquid comprises the following steps:
(I) inoculating azospirillum into a liquid culture medium, and culturing for 36h under the conditions of 30 ℃ and 180r/min, wherein the liquid culture medium comprises the following components in parts by weight: 10 parts of cane sugar, 0.1 part of dipotassium phosphate, 0.2 part of magnesium sulfate heptahydrate, 0.02 part of calcium chloride, 0.002 part of sodium molybdate, 5.0 parts of malic acid, 0.4 part of monopotassium phosphate, 0.1 part of sodium chloride, 0.01 part of ferric chloride and 1000 parts of water; mixing the components, adjusting the pH to 7.0-7.2, then carrying out high-pressure sterilization at 121 ℃ for 30min, and cooling to obtain a liquid culture medium;
(II) centrifuging the liquid culture medium after the inoculation culture in the step (I) to collect thalli, wherein the centrifugation conditions are 4000r/min and 6min, suspending the thalli by using a sterile 3% sucrose solution, and diluting until the concentration of the thalli is 105CFU/ml to obtain the azospirillum liquid.
The base fertilizer of the base fertilizer layer is prepared by mixing the following components in parts by weight: 25 parts of urea, 12 parts of phosphorus pentoxide, 18 parts of potassium nitrate and 30 parts of decomposed straw.
The nitrogen-fixing microbial fertilizer of the nitrogen-fixing microbial fertilizer layer is prepared from the following raw materials in parts by weight: 50 parts of bagasse, 35 parts of vinasse, 8 parts of molasses, 2 parts of yeast, 1 part of nitrogen-fixing blue algae, 2.2 parts of azotobacter chroococcum, 1 part of bacillus megaterium and 1 part of bacillus mucilaginosus. The nitrogen-fixing microbial fertilizer is prepared by the following method: mixing bagasse, vinasse and molasses together to obtain a mixture, controlling the water content of the mixture to be 60-70%, adding activated yeast, performing primary fermentation at 50 ℃ for 3 days, and cooling to room temperature; then adding activated azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus, mixing uniformly, and carrying out secondary fermentation for 2 days at 50 ℃ to obtain the nitrogen-fixing microbial fertilizer.
The organic fertilizer of the organic fertilizer layer comprises the following raw materials in parts by weight: 85 parts of chicken manure, 25 parts of wheat bran, 10 parts of humic acid, 0.8 part of lactic acid bacteria, 0.2 part of aspergillus niger, 8 parts of zeolite powder, 8 parts of bentonite and 10 parts of hydroxypropyl-beta-cyclodextrin. The preparation method of the organic fertilizer layer fertilizer comprises the following steps:
uniformly mixing chicken manure, wheat bran, humic acid and zeolite powder to obtain a mixture, controlling the water content of the mixture to be 50-60%, adding activated lactic acid bacteria and aspergillus niger, and carrying out aerobic fermentation for 5 days at 35 ℃;
drying and granulating the fermented material to obtain fertilizer granules, wherein the grain size of the granules is 2-3 mm;
dissolving hydroxypropyl-beta-cyclodextrin by using water to obtain a cyclodextrin solution, wherein the mass of the water is 2.2 times that of the hydroxypropyl-beta-cyclodextrin; adding the cyclodextrin solution into bentonite, and stirring and mixing uniformly to obtain slurry;
and fourthly, adding the fertilizer particles obtained in the third step into a coating machine, spraying the slurry obtained in the third step under the rotation of the coating machine, spraying the slurry on the surfaces of the fertilizer particles, and drying to form coatings on the surfaces of the fertilizer particles to obtain the organic fertilizer.
Example 3
A planting method for promoting nitrogen fixation of sugarcane is characterized by comprising the following steps:
(1) soaking the single-bud seed stems of the sugarcane in water at 50 ℃ for 1.5h, and then soaking in 800 times of 50% carbendazim solution for 8min to obtain detoxified single-bud seed stems of the sugarcane;
(2) carrying out sand culture on the detoxified sugarcane single-bud seed stems, removing sugarcane seedlings from sand when 3-4 leaves grow out of the sugarcane, cutting off small root parts of the sugarcane seedlings, soaking the root parts of the sugarcane seedlings in azospirillum solution for 30min, wherein the concentration of azospirillum in the azospirillum solution is 105CFU/ml to obtain inoculated sugarcane seedlings;
(3) soil preparation ditching is carried out to the soil of farming, and the science is fertilized in the ditch, the fertilizer contains the three-layer, from the top down is basic fertilizer layer, nitrogen fixation microbial fertilizer layer and organic fertilizer layer in proper order, and basic fertilizer layer is nitrogen phosphorus potassium fertilizer layer, and the rate of fertilizer application: the base fertilizer layer is 300kg/hm2The nitrogen-fixing microbial fertilizer layer is 110kg/hm2The organic fertilizer layer is 220kg/hm2Spreading fine sand on the surface of base fertilizer layer, planting and inoculatingPlanting sugarcane seedlings, wherein the row spacing of the planted sugarcane is 90-100 cm, and the spacing between the planted sugarcane is 20-30 cm;
(4) after 2 months of planting sugarcane seedlings, spraying a nitrogen fixation synergist solution on the sugarcane leaf surfaces, wherein the spraying amount is 60L/mu, and the nitrogen fixation synergist comprises the following components in percentage by weight: 2% of seaweed extract, 1% of fulvic acid, 1.8% of rhamnolipid, 0.1% of molybdic acid, 0.08% of ferric sulfite and the balance of water; diluting the nitrogen fixation synergist into a solution with the concentration of 450ppm by using water to obtain the nitrogen fixation synergist solution.
In the step (2), the preparation method of the azospira liquid comprises the following steps:
(I) inoculating azospirillum into a liquid culture medium, and culturing for 24h at 28 ℃ and 200r/min, wherein the liquid culture medium comprises the following components in parts by weight: 10 parts of cane sugar, 0.1 part of dipotassium phosphate, 0.2 part of magnesium sulfate heptahydrate, 0.02 part of calcium chloride, 0.002 part of sodium molybdate, 5.0 parts of malic acid, 0.4 part of monopotassium phosphate, 0.1 part of sodium chloride, 0.01 part of ferric chloride and 1000 parts of water; mixing the components, adjusting the pH to 7.0-7.2, then carrying out high-pressure sterilization at 121 ℃ for 30min, and cooling to obtain a liquid culture medium;
(II) centrifuging the liquid culture medium after the inoculation culture in the step (I) to collect thalli, wherein the centrifugation conditions are 6000r/min and 3min, suspending the thalli by using a sterile 3% sucrose solution, and diluting until the concentration of the thalli is 105CFU/ml to obtain the azospirillum liquid.
The base fertilizer of the base fertilizer layer is prepared by mixing the following components in parts by weight: 35 parts of urea, 12 parts of phosphorus pentoxide, 18 parts of potassium nitrate and 35 parts of decomposed straw.
The nitrogen-fixing microbial fertilizer of the nitrogen-fixing microbial fertilizer layer is composed of the following raw materials in parts by weight: 50 parts of bagasse, 38 parts of vinasse, 10 parts of molasses, 2 parts of yeast, 1.5 parts of nitrogen-fixing blue algae, 2 parts of azotobacter chroococcum, 1.2 parts of bacillus megaterium and 1.5 parts of bacillus mucilaginosus. The nitrogen-fixing microbial fertilizer is prepared by the following method: mixing bagasse, vinasse and molasses together to obtain a mixture, controlling the water content of the mixture to be 60-70%, adding activated yeast, performing primary fermentation at 50 ℃ for 3 days, and cooling to room temperature; then adding activated azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus, mixing uniformly, and carrying out secondary fermentation for 2 days at 50 ℃ to obtain the nitrogen-fixing microbial fertilizer.
The organic fertilizer of the organic fertilizer layer comprises the following raw materials in parts by weight: 90 parts of chicken manure, 40 parts of wheat bran, 14 parts of humic acid, 1 part of lactic acid bacteria, 0.3 part of aspergillus niger, 10 parts of zeolite powder, 12 parts of bentonite and 12 parts of hydroxypropyl-beta-cyclodextrin. The preparation method of the organic fertilizer layer fertilizer comprises the following steps:
uniformly mixing chicken manure, wheat bran, humic acid and zeolite powder to obtain a mixture, controlling the water content of the mixture to be 50-60%, adding activated lactic acid bacteria and aspergillus niger, and carrying out aerobic fermentation for 5 days at 40 ℃;
drying and granulating the fermented material to obtain fertilizer granules, wherein the grain size of the granules is 2-3 mm;
dissolving hydroxypropyl-beta-cyclodextrin by using water to obtain a cyclodextrin solution, wherein the mass of the water is 3 times that of the hydroxypropyl-beta-cyclodextrin; adding the cyclodextrin solution into bentonite, and stirring and mixing uniformly to obtain slurry;
and fourthly, adding the fertilizer particles obtained in the third step into a coating machine, spraying the slurry obtained in the third step under the rotation of the coating machine, spraying the slurry on the surfaces of the fertilizer particles, and drying to form coatings on the surfaces of the fertilizer particles to obtain the organic fertilizer.
Comparative example 1
This comparative example differs from example 1 in that: the planting method of the sugarcane does not comprise the step (4) that after 3 months of planting the sugarcane seedlings, the sugarcane leaf surfaces are sprayed with the nitrogen fixation synergist solution. The other steps, the composition of the fertilizer and the preparation method are the same as in example 1.
Test examples
5 acres of land with mild terrain and little difference of fertilizer effect ingredients are selected for planting sugarcane and divided into 5 groups, one acre of each group is respectively a group of example 1, a group of example 2, a group of example 3, a group of comparative example 1 and a control group, 5 groups are cultivated uniformly, and the planted sugarcane variety is ROC 22. The groups of examples 1 to 3 are planted according to the planting methods provided in the embodiments 1 to 3 respectively, the group of comparative example 1 is planted according to the planting method provided in the comparative example 1, the groups of examples 1 to 3 and the group of comparative example 1 do not need to be fertilized in the growth period of sugarcane, and other field management is carried out according to a conventional sugarcane field pipeThe processing method is carried out. The control group is planted by applying the traditional sugarcane cultivation method, the sugarcane seedlings are not inoculated, and the fertilization mode is as follows: applying nitrogen, phosphorus and potassium base fertilizer during planting, topdressing nitrogen fertilizer in seedling stage, tillering stage and jointing stage, wherein the nitrogen fertilizer mainly comprises urea, and the application amount of the nitrogen fertilizer is about 500kg/hm2And field management is carried out according to a conventional sugarcane field management method.
And (3) testing items: the method is characterized in that the nitrogenase activity of sugarcane leaves is measured at the initial elongation stage of sugarcane, the purity, the brix, the sugar content and the yield of sugarcane juice are investigated after the maturity stage, and the average value of 3 repetitions is taken for each group of test results.
And (3) test results: table 1 shows the nitrogenase activity of sugarcane leaves at the initial elongation stage of sugarcane, and as can be seen from the table, the nitrogenase activity of the sugarcane is improved by more than 10% in the examples 1-3 groups compared with the control group, the nitrogenase activity of the sugarcane is improved by 4.13% in the comparative example 1 group, the nitrogenase activity can be improved by adopting inoculated azotobacter, and the nitrogenase activity is further improved by spraying a nitrogenase synergist solution, so that the planting method disclosed by the invention can enhance the nitrogenase capacity of the sugarcane.
TABLE 1 Azotose Activity at early elongation of sugarcane
Figure BDA0002475512780000101
Figure BDA0002475512780000111
The cane juice purity, the cane juice brix and the cane sugar content are main basis for examining the quality of the sugarcane, and as can be seen from table 2, the cane juice purity of the example group is more than 91.78%, the cane juice brix is more than 22.3, and the cane sugar content is more than 18.14%, which are improved in different degrees compared with the control group and the comparative group 1. As can be seen from Table 2, the yield of the example group is obviously higher than that of the control group, compared with the control group, the yield increase amplitude is about 14.5-15.0%, and the difference reaches a very significant level. The planting method is beneficial to improving the quality of the sugarcane, so that the yield of the sugarcane is increased remarkably. Compared with the control group, the nitrogen fertilizer application amount of the example group is obviously reduced, and the nitrogen fixation capacity of the sugarcane is improved and the scientific fertilization can reduce the application times and the use amount of the nitrogen fertilizer.
TABLE 2 sugarcane quality index and yield
Figure BDA0002475512780000112
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (8)

1. A planting method for promoting nitrogen fixation of sugarcane is characterized by comprising the following steps:
(1) soaking the sugarcane single-bud seed stems in water at 50 ℃ for 1-2 h, and then soaking in 800 times of 50% carbendazim solution for 5-10 min to obtain detoxified sugarcane single-bud seed stems;
(2) carrying out sand culture on the detoxified sugarcane single-bud seed stems, removing sugarcane seedlings from sand when 3-4 leaves grow out of the sugarcane, cutting off small root parts of the sugarcane seedlings, and soaking the root parts of the sugarcane seedlings in azotobacter liquid for 30-40 min to obtain inoculated sugarcane seedlings;
(3) soil preparation ditching is carried out to the soil of farming, and the science is fertilized in the ditch, the fertilizer contains the three-layer, from the top down is basic fertilizer layer, nitrogen fixation microbial fertilizer layer and organic fertilizer layer in proper order, basic fertilizer layer is nitrogen phosphorus potassium fertilizer layer, and the rate of fertilizer application: the base fertilizer layer is 280-350 kg/hm2The nitrogen-fixing microbial fertilizer layer is 110-150 kg/hm2The organic fertilizer layer is 180-220 kg/hm2Spreading fine sand on the surface of the base fertilizer layer, planting sugarcane seedlings subjected to inoculation, wherein the row spacing of the planted sugarcanes is 90-100 cm, and the spacing between the sugarcane seedlings is 20-30 cm;
(4) after sugarcane seedlings are planted for 2-3 months, spraying a nitrogen fixation synergist solution on sugarcane leaf surfaces, wherein the spraying amount is 50-65L/mu, and the nitrogen fixation synergist comprises the following components in percentage by weight: 1-5% of seaweed extract, 1-2% of fulvic acid, 1-2% of rhamnolipid, 0.05-0.1% of molybdic acid, 0.05-0.08% of ferric sulfite and the balance of water; the nitrogen fixation synergist solution is prepared by diluting a nitrogen fixation synergist with water to form a solution with the concentration of 400-500 ppm.
2. The planting method according to claim 1, wherein in the step (2), the nitrogen-fixing bacteria liquid is a nitrogen-fixing spirochete liquid, and the concentration of the nitrogen-fixing spirochete in the nitrogen-fixing spirochete liquid is 105~106CFU/ml。
3. The planting method according to claim 2, wherein the preparation method of the azospirillum solution comprises the following steps:
（
Figure 973177DEST_PATH_IMAGE001
) Inoculating azospirillum into a liquid culture medium, and culturing for 24-48 h at the temperature of 28-30 ℃ and the speed of 180-200 r/min, wherein the liquid culture medium comprises the following components in parts by weight: 10 parts of cane sugar, 0.1 part of dipotassium phosphate, 0.2 part of magnesium sulfate heptahydrate, 0.02 part of calcium chloride, 0.002 part of sodium molybdate, 5.0 parts of malic acid, 0.4 part of monopotassium phosphate, 0.1 part of sodium chloride, 0.01 part of ferric chloride and 1000 parts of water; mixing the components, adjusting the pH to 7.0-7.2, then carrying out high-pressure sterilization at 121 ℃ for 30min, and cooling to obtain a liquid culture medium;
（
Figure 317571DEST_PATH_IMAGE002
) To the step (A), (B)
Figure 81128DEST_PATH_IMAGE001
) Centrifuging the liquid culture medium after inoculation culture to collect thalli, wherein the centrifugation conditions are 4000-6000 r/min and 3-6 min, suspending the thalli by using a sterile 3% sucrose solution, and diluting until the concentration of the thalli is105~106CFU/ml to obtain the azospirillum liquid.
4. The planting method of claim 1, wherein the base fertilizer of the base fertilizer layer is prepared by mixing the following components in parts by weight: 20-35 parts of urea, 10-15 parts of phosphorus pentoxide, 10-18 parts of potassium nitrate and 20-35 parts of decomposed straw.
5. The planting method of claim 1, wherein the nitrogen-fixing microbial fertilizer of the nitrogen-fixing microbial fertilizer layer is composed of the following raw materials in parts by weight: 40-50 parts of bagasse, 30-40 parts of vinasse, 5-10 parts of molasses, 1-2 parts of saccharomycetes, 1-1.5 parts of nitrogen-fixing blue algae, 2-2.5 parts of azotobacter chroococcum, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus mucilaginosus;
the nitrogen-fixing microbial fertilizer is prepared by the following method: uniformly mixing bagasse, vinasse and molasses to obtain a mixture, controlling the water content of the mixture to be 60-70%, adding activated yeast, performing primary fermentation at 50-60 ℃ for 2-3 days, and cooling to room temperature; and then adding activated azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus, uniformly mixing, and performing secondary fermentation at 40-50 ℃ for 2-3 days to obtain the nitrogen-fixing microbial fertilizer.
6. The planting method of claim 1, wherein the organic fertilizer of the organic fertilizer layer comprises the following raw materials in parts by weight: 80-90 parts of chicken manure, 20-40 parts of wheat bran, 10-15 parts of humic acid, 0.5-1 part of lactic acid bacteria, 0.1-0.3 part of Aspergillus niger, 6-12 parts of zeolite powder, 6-12 parts of bentonite and 6-12 parts of hydroxypropyl-beta-cyclodextrin.
7. The planting method of claim 6, wherein the preparation method of the organic fertilizer comprises the following steps:
Figure 383933DEST_PATH_IMAGE003
uniformly mixing chicken manure, wheat bran, humic acid and zeolite powder to obtain a mixture, and controlling the water content of the mixture to be 50 to60 percent of activated lactobacillus and aspergillus niger are added, and aerobic fermentation is carried out for 4 to 5 days at the temperature of between 35 and 45 ℃;
Figure 600151DEST_PATH_IMAGE004
drying and granulating the fermented material to obtain fertilizer granules, wherein the grain size of the granules is 2-3 mm;
Figure 748235DEST_PATH_IMAGE005
dissolving hydroxypropyl- β -cyclodextrin in water to obtain a cyclodextrin solution, wherein the mass of the water is 2-3 times that of the hydroxypropyl- β -cyclodextrin, adding bentonite into the cyclodextrin solution, and uniformly stirring and mixing to obtain slurry;
Figure 631878DEST_PATH_IMAGE006
will be described in detail
Figure 840005DEST_PATH_IMAGE004
Adding the fertilizer granules into a coating machine, and spraying the fertilizer granules into the coating machine under the rotation of the coating machine
Figure 809098DEST_PATH_IMAGE005
And spraying the obtained slurry on the surface of fertilizer particles, drying, and forming a coating on the surface of the fertilizer particles to obtain the organic fertilizer layer fertilizer.
8. The planting method of claim 1, wherein the nitrogen fixation synergist comprises the following components in percentage by weight: 3-5% of seaweed extract, 1.5-2% of fulvic acid, 1-1.5% of rhamnolipid, 0.05-0.08% of molybdic acid, 0.05-0.06% of ferric sulfite and the balance of water.
CN202010362625.8A 2020-04-30 2020-04-30 Planting method for promoting nitrogen fixation of sugarcane Active CN111567350B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010362625.8A CN111567350B (en) 2020-04-30 2020-04-30 Planting method for promoting nitrogen fixation of sugarcane

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010362625.8A CN111567350B (en) 2020-04-30 2020-04-30 Planting method for promoting nitrogen fixation of sugarcane

Publications (2)

Publication Number Publication Date
CN111567350A true CN111567350A (en) 2020-08-25
CN111567350B CN111567350B (en) 2022-05-17

Family

ID=72110166

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010362625.8A Active CN111567350B (en) 2020-04-30 2020-04-30 Planting method for promoting nitrogen fixation of sugarcane

Country Status (1)

Country Link
CN (1) CN111567350B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112189565A (en) * 2020-11-11 2021-01-08 广西壮族自治区农业科学院 Method for introducing azotobacter into sugarcane tissue culture seedlings
CN112219669A (en) * 2020-09-02 2021-01-15 广西南亚热带农业科学研究所 Simple cultivation method for sugarcane seedlings
CN113367039A (en) * 2021-06-30 2021-09-10 广西壮族自治区农业科学院 Method for planting sugarcane by utilizing sugarcane combustion ash
CN114158449A (en) * 2021-12-29 2022-03-11 广西壮族自治区农业科学院 Method for inducing biological nitrogen fixation of sugarcane
CN114431100A (en) * 2021-12-30 2022-05-06 广西壮族自治区农业科学院 Sugarcane planting method capable of reducing weight and improving efficiency
CN114557252A (en) * 2022-02-28 2022-05-31 广西壮族自治区农业科学院 Planting method for promoting root and strengthening seedlings of sugarcane

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105265155A (en) * 2015-11-09 2016-01-27 邓万超 Sugarcane high-yield planting method
CN107337534A (en) * 2017-08-07 2017-11-10 合肥恒益生态农业科技有限公司 A kind of Mulberry fertilizer of soil improvement type and preparation method thereof
CN108640782A (en) * 2018-08-15 2018-10-12 南京康之春生物科技有限公司 A kind of novel plant azotobacteria fertilizer and preparation method thereof
AU2017249148A1 (en) * 2016-04-15 2018-10-25 Syngenta Participations Ag Methods of producing sugar cane transplant units
CN109516881A (en) * 2019-01-10 2019-03-26 四川省兰月科技有限公司 Biological nitrogen fixation synergist and its application method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105265155A (en) * 2015-11-09 2016-01-27 邓万超 Sugarcane high-yield planting method
AU2017249148A1 (en) * 2016-04-15 2018-10-25 Syngenta Participations Ag Methods of producing sugar cane transplant units
CN107337534A (en) * 2017-08-07 2017-11-10 合肥恒益生态农业科技有限公司 A kind of Mulberry fertilizer of soil improvement type and preparation method thereof
CN108640782A (en) * 2018-08-15 2018-10-12 南京康之春生物科技有限公司 A kind of novel plant azotobacteria fertilizer and preparation method thereof
CN109516881A (en) * 2019-01-10 2019-03-26 四川省兰月科技有限公司 Biological nitrogen fixation synergist and its application method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
刘爱民: "《生物肥料应用基础》", 31 January 2007, 东南大学出版社 *
张强等: "生物刺激素及其在功能水溶性肥料中应用前景分析", 《农业资源与环境学报》 *
王红旗等: "《污染土壤生物修复丛书土壤微生物对石油烃的吸附摄取与跨膜运输》", 30 September 2015, 中国环境科学出版社 *
郑先福: "《植物生长调节剂应用技术》", 30 June 2013, 中国农业大学出版社 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112219669A (en) * 2020-09-02 2021-01-15 广西南亚热带农业科学研究所 Simple cultivation method for sugarcane seedlings
CN112189565A (en) * 2020-11-11 2021-01-08 广西壮族自治区农业科学院 Method for introducing azotobacter into sugarcane tissue culture seedlings
CN113367039A (en) * 2021-06-30 2021-09-10 广西壮族自治区农业科学院 Method for planting sugarcane by utilizing sugarcane combustion ash
CN114158449A (en) * 2021-12-29 2022-03-11 广西壮族自治区农业科学院 Method for inducing biological nitrogen fixation of sugarcane
CN114431100A (en) * 2021-12-30 2022-05-06 广西壮族自治区农业科学院 Sugarcane planting method capable of reducing weight and improving efficiency
CN114557252A (en) * 2022-02-28 2022-05-31 广西壮族自治区农业科学院 Planting method for promoting root and strengthening seedlings of sugarcane

Also Published As

Publication number Publication date
CN111567350B (en) 2022-05-17

Similar Documents

Publication Publication Date Title
CN111567350B (en) Planting method for promoting nitrogen fixation of sugarcane
AU2020101394A4 (en) Microbial fertilizer with double effects of fertilization and disease-resistance, and preparation method and use thereof
CN111302856B (en) Water-soluble organic biological bacterial fertilizer for drip irrigation crops and preparation method thereof
CN110590447A (en) Acidic microbial agent, preparation method and application thereof
CN113200794A (en) Method for improving and repairing soil by using quaternary method
CN111499430A (en) Humic acid compound fertilizer for improving tobacco-planting soil and preparation method thereof
US20240002308A1 (en) Soil-structure improving bio-organic fertilizer and preparation method thereof
CN109438079B (en) Soluble biological organic fertilizer and production method thereof
CN111499457A (en) Peat humic acid type functional microbial fertilizer and preparation method thereof
CN111423279A (en) Composite biological fertilizer for grain crops and preparation method thereof
CN114158450B (en) Overground and underground combined control method for sugarcane ratoon yellowing
CN107974423B (en) Soil biological activator and preparation method thereof
CN115119701A (en) Rice cultivation method using straw compost and environment-friendly enzyme as base fertilizer
AU2020104040A4 (en) A bio-fertilizer for alleviating the obstacle of continuous cropping of GOBI watermelon
CN115010538A (en) Two-component composite microorganism water-retention temperature-control slow-release fertilizer and preparation method and application thereof
CN113773125A (en) Agricultural microbial agent and preparation method thereof
CN112174743A (en) Biological organic fertilizer
CN112592241A (en) Composite biological organic fertilizer with water retention function and preparation method thereof
CN111592402A (en) Method for improving fermentation efficiency of pig manure
CN110981588A (en) Novel liquid rooting agent and preparation method thereof
CN110683911A (en) Biochar-based aflatoxin prevention and control slow-release fertilizer and preparation method thereof
CN106576805B (en) Sweet potato vigorous-growth-controlling and yield-increasing regulator and preparation method and application thereof
CN109608279A (en) A kind of sweetened special fertilizer of tomato and its application method
CN117247295B (en) Organic fertilizer prepared from molasses fermentation liquid and preparation method thereof
CN114409460A (en) Biological organic fertilizer for promoting crop growth and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant