CN113773125A - Agricultural microbial agent and preparation method thereof - Google Patents
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Abstract
The invention relates to an agricultural microbial agent containing nitrogen-fixing red bacteria, lactobacillus plantarum, powdery pichia pastoris and amino acid thereof, and maltitol, and a preparation method and application thereof. The invention takes marine trash fish and soybean meal powder with rich protein content as main raw materials, and takes maltitol and molasses powder as auxiliary materials to decompose different proteins in the raw materials under specific fermentation conditions after reasonable compounding, thereby preparing the agricultural microbial agent taking various thalli, amino acids, polysaccharides and the like as high-efficiency components. The agricultural microbial agent effectively improves the soil micro-ecological environment, promotes the transformation of soil organic matters, is beneficial to various bacteriostatic active substances generated by microorganisms, can enhance the capability of crops in resisting various diseases, reduces the occurrence of soil-borne diseases, and promotes the robust growth of plants. Can effectively promote the formation of soil aggregate structure, make the soil become loose and fertile, and is beneficial to cultivation and the growth and development of crop roots. The product contains rich organic matters, can activate the soil of a plough layer, improve the water retention performance of the soil, simultaneously enhance the adsorption effect of the organic matters, absorb and contain various nutrient substances, effectively avoid nutrient loss, improve the utilization rate of the fertilizer, and enhance the fertilizer retention and supply capacity and the buffer capacity of the soil.
Description
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to an agricultural microbial agent and a preparation method thereof.
Background
At present, the soil all over the world faces the problems of soil property deterioration, soil acidification, salinization, soil microorganism diversity destruction and the like, and the soil impoverishment, soil failure and the like directly influence the sustainable development of agriculture. Under such a severe condition of soil ecology, how to improve soil, improve soil fertility, improve soil ecological environment, and improve crop yield and quality has become the central importance of current agricultural work.
The microorganisms can promote the release and chelation of trace elements in the soil through the exchange of oxygen and carbon dioxide and the secreted acidic substances such as organic acid and the like in the metabolic process, effectively break the soil hardening, promote the formation of granular structures, improve the ventilation condition of the soil and promote the generation of organic matters, humic acid and humus. The problems of weak permeability, poor water and fertilizer retention, poor soil fertility and the like in the current soil are influenced by the granular structure, organic matters, humic acid and humus in the soil, and the formation of the substances is influenced by microorganisms in the soil. Therefore, microorganisms play a very important role in the input and output of materials and energy in soil, and are an important link in the material circulation chain. It can activate organic and inorganic nutrients in soil, decompose organic matter, release nutrients and increase the effectiveness of nutrients. The current causes of the problems of hardening, acidification, salinization, soil impoverishment, soil failure and the like in soil are that fertilizers are accumulated and remained in the soil for a long time and cannot be effectively decomposed and subjected to nutrient conversion, and the nutrient release and conversion are just dependent on microorganisms. During the propagation and metabolism of the microorganisms, the microorganisms can degrade residual fertilizers, organic pesticides, heavy metals and other pollutants in the soil, decompose, convert, fix and transfer the pollutants in the physicochemical reaction of the microorganisms, and decompose the pollutants into low-harmful or even harmless substances, thereby reducing the degree of soil pollution. In addition, microorganisms in the soil, such as antibiotic microorganisms, can secrete antibiotics to inhibit the propagation of pathogenic microorganisms, so that the harm of soil-borne microorganisms in the soil to crops can be prevented, reduced and the quality and yield of the crops can be improved.
The agricultural microbial agent is a solid microbial agent prepared by industrially producing microbes to obtain solid bacterial powder and adsorbing the solid bacterial powder by a certain carrier; the agricultural microbial agent liquid preparation is prepared by mixing single microbial fermentation liquor or multiple microbial fermentation liquors obtained after industrial fermentation according to a certain proportion. But the existing single microbial inoculum has single action and undesirable effect; some composite microbial agents have poor stability due to poor synergistic capability of selected strains, and cannot play a corresponding role.
Disclosure of Invention
The invention aims to provide a composite agricultural microbial agent with strong adaptability to survival and propagation of flora, which is suitable for planting crops, aiming at the problems of soil hardening and fertility decline in the planting of the crops.
The purpose of the invention is achieved by the following technical scheme:
an agricultural microbial agent, which is characterized in that: the agricultural microbial agent comprises the following raw materials: photosynthetic bacteria nitrogen-fixing red bacteria, lactobacillus plantarum, powdery pichia pastoris, amino acid and maltitol.
In another aspect of the present invention, there is provided a method for preparing the microbial agent, comprising the steps of:
(1) preparing a seed solution: respectively inoculating azotobacter cultured by RCVBN slant culture medium, lactobacillus plantarum cultured by MRS slant culture medium and powdery pichia pastoris cultured by YED slant culture medium into seed culture medium, culturing at 25-35 ℃ for 48-96 h to respectively obtain seed solutions of azotobacter, lactobacillus plantarum and powdery pichia pastoris, wherein the azotobacter and the lactobacillus plantarum areThe number of effective viable bacteria of the powdery pichia pastoris seed liquid is more than or equal to 1 multiplied by 1010CFU/mL；
(2) Preparation of the pretreatment mixture: adding water into 40-80 parts of soybean meal and 5-20 parts of marine fishes by weight to adjust the water content to 70-80 wt%, standing for 8-24 hours at 20-35 ℃, then stewing for 20-40min at the temperature of 115 ℃ and 121 ℃, cooling, adding 10-20 parts of sugar honey powder and 15-30 parts of maltitol, and uniformly mixing to obtain a pretreated mixture for later use;
(3) liquid state fermentation culture: and (2) carrying out treatment on the seed liquid of the nitrogen-fixing rhodobacter, the lactobacillus plantarum and the powdery pichia pastoris obtained in the step (1) according to the viable count of 2: 1.5: 1 proportion to obtain mixed bacteria liquid, transferring the mixed bacteria liquid into a pretreated mixture according to the volume ratio of 5-10%, and fermenting and culturing at 25-35 ℃ for 48-96 h to obtain the agricultural microbial agent.
In the above technical solution, further, the RCVBN slant culture medium of step (1) has a formula of: mixing DL-apple 4.0g and (NH)4)2SO4 1.0g、KH2PO41.36g, EDTA 0.02g, vitamin B10.001g, nicotinic acid 0.001g, K2HPO4.3H2O1.24g、MgSO4.7H2O 0.12g、CaCl2.2H20.075 g of O, 0.0028g of boric acid, ZnSO4.7H2O 0.00024g、MnSO4.H2O 0.0016g、Cu(NO3)2.3H2O 0.00004g、Na2MoO4.2H2Adding water 0.00075g and agar powder 10g into the mixture, supplementing water to 1000mL, adjusting pH to 6.8-7.0, stirring, and sterilizing at 121 deg.C and 0.1Mpa for 20 min.
The formula of the MRS slant culture medium in the step (1) is that 10.0g of peptone, 10.0g of beef extract, 5.0g of yeast extract, 20.0g of glucose, 5.0g of sodium acetate, 2.0g of diamine citrate, 801.0g of tween-801.0 g, 0.4g of dipotassium phosphate, 0.58g of magnesium sulfate, 0.29g of manganese sulfate, 20.0g of calcium carbonate and 15.0g of agar are sequentially added into water, the water is supplemented to 1000mL, the pH value is adjusted to 6.3, the mixture is stirred, heated, boiled for 2min and sterilized for 20min at the temperature of 121 ℃ and the pressure of 0.1 MPa.
The YED culture medium formula in the step (1) is that 20g of tryptone, 20g of glucose and 2g of agar powder are sequentially added with distilled water, the distilled water is supplemented to 1000mL, and the mixture is sterilized at 115 ℃ for 15 min.
The seed liquid culture medium formula of the step (1) comprises the following components in percentage by weight: 10-15% of molasses powder, 5-10% of soybean meal powder, 15-30% of maltitol and the balance of water.
An agricultural microbial preparation is prepared by the above method, and its total effective viable count is 2 × 1010 _3×1010CFU/mL, and the mass content of free amino acid is 5-15 g/L.
The invention provides an application method of the agricultural microbial agent, which comprises the following steps: the fertilizer is used for soil improvement or yield increase of vegetables, melons, fruit trees and grain crops or is compounded with a compound fertilizer and an organic-inorganic compound fertilizer to be used as a fertilizer synergist;
preferably, according to the present invention, when used for soil improvement, the application method comprises: mixing an agricultural microbial agent in a mass ratio of 1: diluting with 50-200% water to obtain a diluent, and uniformly spraying the diluent on the soil surface before planting the crops until the diluent is thoroughly sprayed to the soil depth of 20 cm; when the microbial agent is used for increasing the yield of vegetables, melons, fruit trees and grain and cotton crops, the agricultural microbial agent is mixed with soil according to the mass ratio of 1:15, then the mixture is applied to planting holes or sowing holes, and then seedlings are transplanted or sowed;
according to the invention, when the fertilizer synergist is used, 50-100kg of agricultural microbial agent is preferably added to each ton of fertilizer.
The invention has the beneficial effects that:
1. the microbial agent contains a large amount of high-activity photosynthetic bacteria, namely azotobacter, lactobacillus plantarum and powdery pichia pastoris, and the number of effective live bacteria can reach as high as 50 hundred million/mL.
The microbial inoculum product prepared by the invention contains billions of effective viable bacteria per gram, wherein the nitrogen-fixing red bacteria is photosynthetic bacteria, has the nitrogen-fixing effect and increases the soil fertility. The lactobacillus plantarum can adjust the pH value of soil, the powdery pichia pastoris can be quickly propagated into a dominant flora when being applied into the soil, the physicochemical properties of the soil are adjusted, and the amount of soil microorganisms is increased.
2. The microbial agent contains rich animal source and plant source free amino acids and rich strain metabolites.
The microbial agent is fermented by using marine fishes and soybean meal powder, is rich in a large amount of free amino acids, is easy to absorb by crops, promotes the synthesis of dry matters, ensures that the product has thicker color and luster, larger size, higher VC and sugar content, and greatly improves the yield and quality of the crops. The combination of polysaccharide and other substances in the microbial inoculum and nutrients in the fertilizer can reduce the loss of nitrogen, phosphorus and potassium nutrients of the chemical fertilizer and the fixation of phosphorus elements, promote the transport rate of the nutrient elements in crops, and improve the absorption and utilization efficiency of the crops on the fertilizer.
3. The preparation method has simple process and short production period. The invention uses soybean meal powder, molasses, marine trash fish and the like as raw materials, does not need chemical process treatment and additional enzyme enzymolysis, is completely realized by microbial fermentation in the whole production process, does not need complicated energy-consuming post-treatment processes such as separation and purification, and also does not need additional living microorganisms, thereby greatly reducing the production cost.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further clearly explained below with reference to specific examples, but is not to be construed as being limited in any way. The experimental methods in the following examples are all conventional methods unless otherwise specified; the percentages in the following examples are by mass unless otherwise specified.
Rhodobacter azotoformans (Rhodobacter azotoformans) strain CGMCC NO.1.5025, Lactobacillus plantarum (Lactobacillus plantarum) strain CGMCC NO.1.2469, all purchased from China general microbiological culture Collection center; pichia farinosa (Pichia farinosa) CCTCC CY 20081075 strain was purchased from China center for type culture Collection.
The RCVBN slant culture medium comprises the following components: mixing DL-apple 4.0g and (NH)4)2SO4 1.0g、KH2PO41.36g, EDTA 0.02g, vitamin B10.001g, nicotinic acid 0.001g, K2HPO4.3H2O1.24g、MgSO4.7H2O 0.12g、CaCl2.2H20.075 g of O, 0.0028g of boric acid, ZnSO4.7H2O 0.00024g、MnSO4.H2O 0.0016g、Cu(NO3)2.3H2O 0.00004g、Na2MoO4.2H2Sequentially adding 0.00075g of O and 10g of agar powder into water, supplementing the water to 1000mL, adjusting the pH to 6.8-7.0, stirring, and sterilizing at 121 ℃ and 0.1Mpa for 20 min;
the MRS slant culture medium is prepared by sequentially adding 10.0g of peptone, 10.0g of beef extract, 5.0g of yeast extract, 20.0g of glucose, 5.0g of sodium acetate, 2.0g of diamine citrate, 801.0g of tween-801.0 g, 0.4g of dipotassium hydrogen phosphate, 0.58g of magnesium sulfate, 0.29g of manganese sulfate, 20.0g of calcium carbonate and 15.0g of agar into water, supplementing water to 1000mL, adjusting pH to 6.3, stirring, heating, boiling for 2min, and sterilizing at 121 ℃ and 0.1MPa for 20 min;
the YED culture medium formula comprises that tryptone 20g, glucose 20g and agar powder 2g are sequentially added into water, the water is supplemented to 1000mL, and the mixture is sterilized at 115 ℃ for 15 min.
Example 1: a preparation method of a polypeptide agricultural microbial agent comprises the following steps:
(1) preparing a seed solution: respectively inoculating azotobacter cultured by RCVBN slant culture medium, Lactobacillus plantarum cultured by MRS slant culture medium and Pichia pastoris powder cultured by YED slant culture medium into seed culture medium, culturing at 28 deg.C for 96 hr to respectively obtain seed solutions of azotobacter, Lactobacillus plantarum and Pichia pastoris, wherein the effective viable count of the seed solutions is 3 × 1010CFU/mL、2×1010CFU/mL、1×1010CFU/mL；
(2) Preparation of the pretreatment mixture: adding water into 40 parts of soybean meal and 20 parts of marine trash fish by weight to adjust the water content to 70 wt%, standing for 12 hours at 28 ℃, then cooking for 20 minutes at 115 ℃, cooling, adding 20 parts of molasses powder and 15 parts of maltitol, and uniformly mixing to obtain a pretreated mixture for later use;
the formula of the seed liquid culture medium is as follows: the weight percentage of the molasses powder is 10 percent, the soybean meal powder is 10 percent, the maltitol is 30 percent, and the rest is water;
(3) liquid state fermentation culture: and (2) carrying out treatment on the seed liquid of the nitrogen-fixing rhodobacter, the lactobacillus plantarum and the powdery pichia pastoris obtained in the step (1) according to the viable count of 2: 1.5: 1 proportion to obtain mixed bacteria liquid, transferring the mixed bacteria liquid into the pretreated mixture according to the volume ratio of 10 percent, and performing fermentation culture at the temperature of 28 ℃ for 72 hours to obtain the agricultural microbial agent.
Example 2: a preparation method of a polypeptide agricultural microbial agent comprises the following steps:
(1) preparing a seed solution: respectively inoculating azotobacter cultured by RCVBN slant culture medium, Lactobacillus plantarum cultured by MRS slant culture medium and Pichia pastoris powder cultured by YED slant culture medium into seed culture medium, culturing at 30 deg.C for 72 hr to respectively obtain seed solutions of azotobacter, Lactobacillus plantarum and Pichia pastoris, wherein the effective viable count of the azotobacter, Lactobacillus plantarum and Pichia pastoris seed solution is 2 × 1010CFU/mL、1×1010CFU/mL、1×1010CFU/mL。
(2) Preparation of the pretreatment mixture: adding water into 50 parts of soybean meal and 15 parts of marine fishes by weight to adjust the water content to 80 wt%, standing for 24 hours at 25 ℃, then cooking for 20 minutes at 121 ℃, cooling, adding 10 parts of molasses powder and 30 parts of maltitol, and uniformly mixing to obtain a pretreated mixture for later use;
the formula of the seed liquid culture medium is as follows: the weight percentage of the components is 15 percent of molasses powder, 10 percent of soybean meal powder, 15 percent of maltitol and the balance of water.
(3) Liquid state fermentation culture: and (2) carrying out treatment on the seed liquid of the nitrogen-fixing rhodobacter, the lactobacillus plantarum and the powdery pichia pastoris obtained in the step (1) according to the viable count of 2: 1.5: 1 proportion to obtain mixed bacteria liquid, transferring the mixed bacteria liquid into the pretreated mixture according to the volume ratio of 5 percent, and fermenting and culturing for 60 hours at the temperature of 30 ℃ to obtain the agricultural microbial agent.
Comparative example 1:
a method of preparing an agricultural microbial inoculant, as described in example 1, except that: in the method, only the nitrogen-fixing red bacteria seed liquid is inoculated for fermentation, and the fermentation conditions are the same as those of the example 1.
Comparative example 2:
a method of preparing an agricultural microbial inoculant, as described in example 1, except that: in the method, only lactobacillus plantarum seed liquid is inoculated for fermentation, and the fermentation conditions are the same as in example 1.
Comparative example 3:
a method of preparing an agricultural microbial inoculant, as described in example 1, except that: in the method, only powdery pichia pastoris seed liquid is inoculated for fermentation, and the fermentation conditions are the same as those in example 1.
Test example 1
In the rice planting field of the west coast village in the northern city of Guangxi Yulin city, 12 mu of rice fields are selected and randomly divided into 6 groups, each group is 2 mu, and the groups are respectively an embodiment group and a control group, wherein the control group uses common urea base fertilizer, the embodiment group and the control group use urea base fertilizer, and then the embodiment group and the control group use the microbial agent for rice planting.
A fertilizing mode:
the control group is base fertilizer urea of 10 kg;
the example group and the control group are applied with urea at the same bottom, and then 50 kg/mu of microbial inoculum is used in the field turning period, 50 kg/mu of microbial inoculum is used in the seedling stage (seedlings grow to about 15 cm), 15 kg/mu of microbial inoculum is used in the tillering period, and 10 kg/mu of microbial inoculum is used in the flowering period (flowering but not heading).
The rice planting varieties are as follows: yusi No. 6, normal field management.
The plant height and yield of the rice are counted, the results are averaged, and the planting effect of the example group and the control group is shown in
Table 1.
| Item | Plant height (cm) | Yield (kg/mu) |
| Example 1 | 9.54 | 670 |
| Example 2 | 9.72 | 660 |
| Comparative example 1 | 7.15 | 610 |
| Comparative example 2 | 6.98 | 590 |
| Comparative example 3 | 7.24 | 605 |
| Control group | 6.52 | 590 |
As can be seen from the results in Table 1, by applying the microbial inoculum of the invention to the rice planting soil, compared with a control group, the yield is increased by more than 70kg per mu, the yield is increased by more than 11.8 percent, and the yield is obviously higher than that of the control group; compared with the comparative example, the yield is obviously increased.
Test example 2
3-year guava test in west village: let 5 treatments in total for examples 1-2 and comparative examples 1-3. Each treatment contains 5 guava trees, the dosage of the microbial inoculum products of the examples 1-2 and the comparative examples 1-3 is that each plant is drenched by irrigating roots with 50g of agricultural microbial inoculum and adding 500 times of water, and meanwhile, the non-applied microbial inoculum is taken as a comparative example. The other fertilizers are applied uniformly according to the habit of farmers, and all management measures are consistent. The guava variety is pearl guava, and the application effect is shown in table 2.
TABLE 2 application Effect of guava
| Item | Yield (kg/mu) | Percentage of anthracnose fruit disease (%) |
| Example 1 | 4958 | 5 |
| Example 2 | 4990 | 4 |
| Comparative example 1 | 4538 | 11 |
| Comparative example 2 | 4376 | 9 |
| Comparative example 3 | 4497 | 10 |
| Control group | 4102 | 15 |
As can be seen from the results in Table 2, the microbial agent provided by the invention is applied to the guava root irrigation, compared with a control group, the yield is increased by over 800kg per mu, the yield is increased by over 20.8%, and the yield is obviously higher than that of the control group; compared with the comparative example, the yield is obviously increased. Meanwhile, after the microbial agent is applied, the incidence rate of fruit anthracnose is reduced.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (8)
1. A preparation method of an agricultural microbial agent is characterized by comprising the following steps: the method comprises the following steps:
(1) preparing a seed solution: respectively inoculating nitrogen-fixing red bacteria cultured by an RCVBN slant culture medium, lactobacillus plantarum cultured by an MRS slant culture medium and powdery pichia pastoris cultured by a YED slant culture medium into seed culture media, culturing for 48-96 h at 25-35 ℃, and respectively obtaining seed solutions of the nitrogen-fixing red bacteria, the lactobacillus plantarum and the powdery pichia pastoris, wherein the effective viable count of the nitrogen-fixing red bacteria, the lactobacillus plantarum and the powdery pichia pastoris is not less than 1 multiplied by 1010CFU/mL；
(2) Preparation of the pretreatment mixture: adding water into 40-80 parts of soybean meal and 5-20 parts of marine fishes by weight to adjust the water content to 70-80 wt%, standing for 8-24 hours at 20-35 ℃, then stewing for 20-40min at 121 ℃ of 115-;
(3) liquid state fermentation culture: and (2) carrying out treatment on the seed liquid of the nitrogen-fixing rhodobacter, the lactobacillus plantarum and the powdery pichia pastoris obtained in the step (1) according to the viable count of 2: 1.5: 1, mixing in proportion to obtain mixed bacteria liquid; and transferring the mixed bacteria liquid into the pretreated mixture according to the volume ratio of 5-10%, and carrying out fermentation culture at 25-35 ℃ for 48-96 h to obtain the agricultural microbial agent.
2. The method for preparing an agricultural microbial inoculum according to claim 1, wherein the formulation of the RCVBN slant culture medium in the step (1) is as follows: mixing DL-apple 4.0g and (NH)4)2SO4 1.0g、KH2PO41.36g, EDTA 0.02g, vitamin B10.001g, nicotinic acid 0.001g, K2HPO4.3H2O1.24g、MgSO4.7H2O 0.12g、CaCl2.2H20.075 g of O, 0.0028g of boric acid, ZnSO4.7H2O 0.00024g、MnSO4.H2O 0.0016g、Cu(NO3)2.3H2O 0.00004g、Na2MoO4.2H2Sequentially adding 0.00075g of O and 10g of agar powder into water, supplementing the water to 1000mL, adjusting the pH to 6.8-7.0, stirring, and sterilizing at 121 ℃ and 0.1Mpa for 20 min;
the formula of the MRS slant culture medium in the step (1) is that 10.0g of peptone, 10.0g of beef extract, 5.0g of yeast extract, 20.0g of glucose, 5.0g of sodium acetate, 2.0g of diamine citrate, 801.0g of tween-801.0 g, 0.4g of dipotassium phosphate, 0.58g of magnesium sulfate, 0.29g of manganese sulfate, 20.0g of calcium carbonate and 15.0g of agar are sequentially added into water, the water is supplemented to 1000mL, the pH value is adjusted to 6.3, the mixture is stirred, heated, boiled for 2min and sterilized for 20min at the temperature of 121 ℃ and the pressure of 0.1 MPa;
the YED culture medium formula in the step (1) is that tryptone 20g, glucose 20g and agar powder 2g are sequentially added into water, the water is supplemented to 1000mL, and sterilization is carried out at 115 ℃ for 15 min;
the formula of the seed liquid culture medium in the step (1) is as follows: the weight percentage is as follows: 10-15% of molasses powder, 5-10% of soybean meal powder, 15-30% of maltitol and the balance of water.
3. An agricultural microbial agent, characterized by being prepared by the method for preparing an agricultural microbial agent according to any one of claims 1 to 2.
4. The agricultural microbial inoculant according to claim 3, wherein the total effective viable count of the agricultural microbial inoculant is 3 x 1010 _5×1010CFU/mL, and the mass content of free amino acid is 5-15 g/L.
5. The agricultural microbial agent of claim 3 is used for soil improvement or yield increase of vegetables, melons, fruit trees and grain crops or is compounded with a compound fertilizer and an organic-inorganic compound fertilizer to be used as a fertilizer synergist.
6. The agricultural microbial agent for soil improvement according to claim 5, wherein the application method comprises the following steps: mixing an agricultural microbial agent in a mass ratio of 1: diluting with 50-200% water to obtain a diluted solution, and uniformly spraying the diluted solution on the soil surface before planting until the diluted solution is thoroughly drenched to the soil depth of 20 cm.
7. The application of the agricultural microbial agent in yield increase of vegetables, melons, fruit trees and grain crops according to claim 5 is characterized in that when the agricultural microbial agent is used for yield increase of the vegetables, the melons, the fruit trees and the grain crops, the agricultural microbial agent is mixed with soil according to the mass ratio of 1:15, then the mixture is applied to planting holes or sowing holes, and then seedlings are transplanted or sowed.
8. The agricultural microbial agent, the compound fertilizer and the organic-inorganic compound fertilizer are compounded to be used as a fertilizer synergist according to claim 5, and the fertilizer synergist is characterized in that 50-100kg of the microbial agent is added to each ton of fertilizer when the fertilizer synergist is used.
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| CN105315997A (en) * | 2014-07-21 | 2016-02-10 | 康源绿洲生物科技（北京）有限公司 | Composite microbial soil repairing agent, and preparation and usage methods thereof |
| CN110818472A (en) * | 2019-12-03 | 2020-02-21 | 山东省农业科学院农业资源与环境研究所 | Vegetable waste liquid microbial fertilizer and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105315997A (en) * | 2014-07-21 | 2016-02-10 | 康源绿洲生物科技（北京）有限公司 | Composite microbial soil repairing agent, and preparation and usage methods thereof |
| CN110818472A (en) * | 2019-12-03 | 2020-02-21 | 山东省农业科学院农业资源与环境研究所 | Vegetable waste liquid microbial fertilizer and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115626847A (en) * | 2022-08-31 | 2023-01-20 | 宁夏大学 | Process for preparing amino acid water-soluble fertilizer by rapid acidolysis of animals died of illness and application |
| CN115626847B (en) * | 2022-08-31 | 2024-04-19 | 宁夏大学 | Process for preparing amino acid water-soluble fertilizer by rapid acidolysis of animals dying of illness and application |
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