CN114158449A - Method for inducing biological nitrogen fixation of sugarcane - Google Patents
Method for inducing biological nitrogen fixation of sugarcane Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of plant nitrogen fixation, and particularly discloses a method for inducing biological nitrogen fixation of sugarcane, which comprises the following steps: (1) applying nitrogen fertilizer according to the soil fertility status to 3-4 true leaves growing after the sugarcane seedlings are aligned, and applying nitrogen fertilizer according to the N: P ratio per mu2O5:K2Applying phosphorus-potassium fertilizer according to the proportion of O =1:0.5:1, and covering soil after applying the fertilizer; (2) the sugarcane is not fertilized any more after being closed, and only needs to keep normal moisture supply. The invention applies nitrogen fertilizer at one time in the early growth stage of sugarcane, wherein the nitrogen application amount is 10-15kg/hm2The fertilizer is not applied after the sugarcane is sealed, the sugarcane can be effectively induced to carry out biological nitrogen fixation, the requirement of the sugarcane on growth is met, the high yield of the sugarcane is ensured, and the sugarcane sugar content of the sugarcane is obviously improved, so that the weight reduction, cost saving and efficiency improvement of the sugarcane production are realized.
Description
Technical Field
The invention belongs to the technical field of plant nitrogen fixation, and particularly relates to a method for inducing biological nitrogen fixation of sugarcane.
Background
Guangxi is the biggest sugar industry base in China, and the yield of cane sugar accounts for about 70 percent of the whole country. The traditional view points that the growth period of the sugarcane is long, the fertilizer requirement is large, particularly the elongation period is the period of sugarcane yield formation, the growth is fast, and the nitrogen fertilizer requirement is maximum. Therefore, the nitrogen application amount in sugarcane production in China is large, generally 750 kilograms of nitrogen (975 1635 kilograms of urea) are applied to each hectare and is more than one time more than the average nitrogen application amount in the world, and besides the base fertilizer, the top dressing is a 'three-attack one-supplement' fertilization method, namely, a small amount of nitrogen fertilizer (5-10%) is applied to attack seedlings in the seedling stage according to the nitrogen demand in different growth periods; the nitrogen application amount (10-15%) is properly increased in the tillering stage to attack tillers, nitrogen fertilizer (more than 70%) is applied again in the elongation stage to attack stems, tail strengthening fertilizer (10%) is applied additionally in the later stage to enhance the vitality, and a large amount of manpower and material resources are consumed for multiple fertilization and earthing up, so that the production cost is high. Moreover, researches prove that the utilization rate of nitrogenous fertilizers for sugarcane production in China is low (only 14.5-24.7%), most of nitrogen is volatilized, eluviated and adsorbed by soil, and a great amount of applied nitrogen is lost, so that the waste is serious, the nitrogen enters the environment to become an important pollution factor, the sustainable development of the sugarcane industry is seriously restricted, and an effective way for losing weight and improving efficiency is urgently needed to be found.
Disclosure of Invention
The invention aims to provide a method for inducing biological nitrogen fixation of sugarcane, so that the defects of excessive fertilizer application, low nitrogen fertilizer utilization rate and environmental pollution in the prior art are overcome, the nitrogen fertilizer utilization rate is improved by inducing biological nitrogen fixation of sugarcane, and technical support is provided for realizing weight reduction and efficiency improvement in sugarcane production.
In order to achieve the above object, the present invention provides a method for inducing biological nitrogen fixation of sugarcane, comprising:
(1) applying nitrogen fertilizer according to the soil fertility status to 3-4 true leaves growing after the sugarcane seedlings are aligned, and applying nitrogen fertilizer according to the N: P ratio2O5:K2Applying a phosphorus-potassium fertilizer according to the proportion of 1:0.5:1, and covering soil after applying the fertilizer;
(2) the sugarcane is not fertilized after the sugarcane is elongated, so that the moisture supply is ensured.
Earlier studies found that the root, stem and leaf of sugarcane all have nitrogen-fixing bacteria to fix nitrogen. The azotase activity is different in different tissues, organs and different growth periods of the sugarcane, wherein the azotase activity is higher in root, stem and leaf sheaths, and a plurality of azotobacteria play a role together, and can be efficiently expressed in roots, stems and leaves. By using15N isotope dilution method detection finds that the combined nitrogen fixation rate and the nitrogen fixation amount of different sugarcane varieties are lower in the seedling stage, are obviously higher than the tillering stage in the initial elongation stage, and the combined nitrogen fixation activity in the whole elongation stage is strongest. Meanwhile, the azotase activity of sugarcane tissues growing under a proper nitrogen level is found to be remarkably higher than that of sugarcane tissues growing under a high nitrogen level, proper nitrogen is provided at the early growth stage of sugarcane, the azotase activity of sugarcane is favorably improved, the azotase activity of sugarcane is remarkably inhibited if the nitrogen application is too high, and especially the azotase activity of sugarcane is seriously inhibited after a large amount of nitrogen fertilizers are applied in the elongation stage. Therefore, the combined nitrogen fixation of the sugarcane can be effectively induced by controlling the application amount of the nitrogen fertilizers in different growth periods. Research shows that the combined nitrogen fixation capacity of sugarcane in the seedling stage is relatively poor, nitrogen needs to be absorbed from soil through root systems to maintain plant growth, and if the nitrogen is not sufficient in the seedling stageThe nitrogen supply of the fertilizer is easy to cause nitrogen deficiency, and the later growth and development are seriously influenced. For varieties with strong nitrogen fixation capacity, such as B1, B8, B9 and the like, nitrogen deficiency symptoms are not easy to appear, but the growth of sugarcane plants is remarkably promoted by applying nitrogen in the seedling stage. Therefore, the period from the seedling stage to the tillering stage is the critical period of the nitrogen nutrient growth of the sugarcane. By the elongation stage, the sugarcane plants grow vigorously in a positive high-temperature rainy season, the combined nitrogen fixation capacity is strong, and nitrogen fertilizers are not required to be applied, so that the weight-losing and efficiency-increasing effects are realized. In contrast, if nitrogen fertilizer is applied in large quantities at this time, the combined nitrogen fixation of sugarcane is severely inhibited. By applying the nitrogen fertilizer and the phosphorus-potassium fertilizer in the seedling stage to the 3-4 leaf stage and adjusting the proportion of the nitrogen, the phosphorus and the potassium, the growth of plants can be effectively promoted, strong roots and stems and leaves are formed, the sugarcane is not fertilized any more after being closed, and the sugarcane is promoted to realize biological nitrogen fixation through thirst and hunger supply.
Preferably, in the step (1), the phosphorus-potassium fertilizer and the trace fertilizer are applied simultaneously. The micro-fertilizer is purchased from the market, can be selected from molybdenum-containing micro-fertilizer, and is applied according to the dosage of the specification.
Preferably, in step (1), the nitrogen fertilizer isQuick-acting nitrogen fertilizerThe micro-fertilizer is a micro-fertilizer for chelating microelements such as iron, zinc, molybdenum and the like, and further, the nitrogen fertilizer is urea.
Preferably, if the micro-fertilizer does not contain molybdenum, ammonium molybdate can be sprayed on the leaf surfaces. Preferably, 0.01-0.02mg/L molybdenum is sprayed on the leaf surfaces.
Preferably, the pH value of the soil is adjusted to 6.8-7.5 before the sugarcane is planted. Preferably, the soil pH is adjusted to 7.
Preferably, before the sugarcane is planted, deep ploughing and deep scarification are carried out on the planting land, and 5-6cm of soil is covered after fertilization in the true leaf period. The depth of the deep scarification is 50-60 cm. The deep ploughing, the deep scarification and the high hilling are beneficial to the formation of a strong root system of the sugarcane, and the early closing of the sugarcane is promoted. The sugarcane forms a strong root system and a vigorous overground part, so that the number of azotobacter in the sugarcane body can be increased, and the nitrogen fixation activity efficiency of the sugarcane is improved.
Preferably, in the step (1), the field management in the sugarcane seedling stage ensures a proper amount of soil moisture, namely, the surface layer of the soil in the seedling stage is 30cm, and the water capacity of the soil in the field is kept to be 60-80%. Tests show that the proper amount of water content is more beneficial to nitrogen fixation of the sugarcane.
Preferably, before the sugarcane is planted, 10% of clothianidin and 40% of bromoacyl-thiamethoxam suspension seed coating agent are adopted to soak the sugarcane seed stems for 8-10 min. And insect-resistant medicines can be applied during sugarcane planting, and mainly prevent sugarcane borers, thrips, woolly aphids, mealybugs and the like. The sugarcane can be prevented insects by soaking the insect-proof seed coating agent and spraying insect-proof medicine during planting, thereby being beneficial to early closing of the sugarcane.
Preferably, the sugarcane is planted by covering with a mulching film and spraying with a herbicide, wherein the herbicide can be at least one of atrazine, acetochlor or diuron. The weed control is beneficial to the growth of the sugarcane.
Preferably, in the step (1), the sugarcane variety is any one of cassia sugar No. 29, cassia sugar No. 42, cassia sugar No. 44, cassia sugar No. 46, cassia sugar No. 49, cassia sugar No. 55, cassia sugar No. 58, cassia sugar No. 60 and cassia willow 05-136.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention concentrates a proper amount of nitrogen fertilizer on the early growth stage of the sugarcane to accelerate the early growth stage, so that the sugarcane plants form strong root systems and strong overground stems and leaves, the sugarcane is sealed as early as possible, and the fertilizer is not applied any more in the large growth period (elongation period) after the sugarcane is sealed, so that the biological nitrogen fixation of the sugarcane is promoted, and the nitrogen nutrition required by the growth of the sugarcane is provided.
2. The method combines the application of the nitrogen fertilizer with the adjustment of soil pH, the supply of water, the supply of phosphorus potassium fertilizer and trace elements, provides a good environment for biological nitrogen fixation of the sugarcane, and is matched with field management measures such as insect prevention, weeding and the like, thereby being beneficial to the growth of the sugarcane and promoting the biological nitrogen fixation of the sugarcane.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
The inventor of the application carries out years of experiments, and the results show that the growth of the sugarcane is inhibited under the condition of not applying nitrogen fertilizer, the combined nitrogen fixation efficiency is very low, and the yield is obviously reduced; when 10kg of urea is applied per mu, the sugarcane of different varieties grows well and is combinedThe nitrogen fixation efficiency is high, and the yield is not obviously different from that of applying 20kg of urea per mu. In this example, two different nitrogen application amounts were designed to perform a multi-year, multi-point, multi-variety test. Namely, the urea is fed to the sugarcane at the 3-leaf stage of the sugarcane at one time, and the N: P is added2O5:K2And (3) applying a phosphorus-potassium fertilizer according to the proportion of 1:0.5:1, and covering 5-6cm of soil after applying the fertilizer. The sugarcane is not fertilized any more in the growth period, so that the water supply is ensured. Watering is carried out in due time according to the climate condition, and the water capacity of the soil in the field is kept to be 60-80% within 30cm of the surface layer of the soil in the seedling stage. And the rest is planted and managed according to a conventional method.
The results show that the yield difference of 10kg of nitrogen applied to each mu of sugarcane and 40kg of sugarcane is small, but the lower nitrogen application amount obviously improves the sugarcane sugar content of the sugarcane; wherein the increase of the content in 11 months is 0.65% (absolute value, the same applies below), the increase of 12 months is 0.70%, the increase of 1 month is 0.39%, and the average increase is 0.58% (Table 1).
TABLE 1 yield and sucrose sub-expression for different sugarcane varieties with different nitrogen application
Note: the data in the table are the average of two year tests in 4 different regions. Data in the same column are labeled with the same lower and upper case letters indicating no significant difference at the 5% and 1% levels.
In the test, a proper amount of nitrogen fertilizer is concentrated in the early growth stage of the sugarcane to accelerate the early growth, so that the sugarcane plants form strong root systems and strong overground stems and leaves, the sugarcane is closed as early as possible, and the fertilizer is not applied any more in the large growth period (elongation period) after the sugarcane is closed, so that the biological nitrogen fixation of the sugarcane is promoted, and the nitrogen nutrition required by the growth of the sugarcane is provided. This is in contrast to conventional fertilization methods: 30-50kg of nitrogen fertilizer is applied, and a separate multi-time fertilization method of 'three attacks and one supplement' is adopted, which has obvious difference. The traditional fertilization mode is broken through, the yield and the quality of the sugarcane are ensured, the environment is friendly, and the sustainable development of sugarcane planting is facilitated.
Example 2
The planting position is located in Li town of Fuseiul county of Chongxi city in Guangxi, the planting field is a region of old sugarcane for many years, and the pH value of soil is 6.5. The planted sugarcane variety is osmanthus sugar No. 46, the planting density is 6000 buds/mu, and different fertilization and management modes are planted in different areas.
Treatment group 1: the sugarcane is planted according to the following method:
(1) land preparation: deep loosening, crushing and soil preparation are carried out on soil by adopting a deep loosening and soil crushing machine, the soil loosening depth is 50-60cm, lime and biochar are turned and pressed into the soil, and the pH value of the planting field is adjusted to be about 7.0. Lime and charcoal can adjust the pH value of soil, kill some plant diseases and insect pests in the soil, improve the porosity of the soil, improve the water retention and air permeability of the soil and facilitate the growth of the root system of the sugarcane in the early stage. And whether pesticides and herbal medicines are sprayed or not can be selected according to the soil condition.
(2) Seed stem treatment: selecting healthy sugarcane seed stems, soaking the healthy sugarcane seed stems in 10% clothianidin and 40% bromacyl-thiamethoxam suspension seed coating agent for 8-10min, and draining water for later use. Both the two medicaments are purchased in the market and used according to the dosage of the specification. The clothianidin is soaked to improve the insect prevention and disease resistance of sugarcane seedlings, and the 40% bromoacyl-thiamethoxam suspension seed coating agent is soaked to promote the rapid production of the sugarcane in the early stage and form a strong root system.
(3) Planting sugarcane: the depth of the ditches is 30-40cm, the sugarcane is planted by adopting a seed sowing mode in a shape like a Chinese character pin, the planting density of the sugarcane is 6000 buds/mu, a mulching film is covered while planting, and meanwhile, insect-proof medicines and weed-control medicines are sprayed to prevent weeds and insects for further preventing insects and weeding.
(4) Field management: applying nitrogen fertilizer to 3-4 true leaves of sugarcane according to soil fertility status at a rate of 10 kg/mu, and simultaneously applying nitrogen fertilizer to sugarcane according to N: P ratio2O5:K2And (3) applying a phosphorus-potassium fertilizer according to the proportion of 2:1:2, and applying a proper amount of a micro-fertilizer according to a product specification, wherein the micro-fertilizer is a micro-fertilizer chelating trace elements such as iron, zinc, molybdenum and the like. And 5-6cm of soil is covered after fertilization. The sugarcane is not fertilized any more in the growth period, so that sufficient water supply is ensured; watering is carried out at proper time according to climatic conditions (artificial watering is carried out for 2 times in the current year of planting), and the water capacity of the soil in the field is kept to be 60-80% within 30cm of the surface layer of the soil in the seedling stage. And the method of combining physics and chemistry is adopted to prevent and control plant diseases and insect pests, such as hanging an insect trap in a sugarcane field to prevent insects and the like.
(5) Harvesting: harvesting the mature sugarcane in time, wherein the whole planting period is 258 days; sugarcane was leaf stripped 1 time before harvest. The ventilation and light transmission among sugarcane rows can be increased through leaf peeling, the occurrence of plant diseases and insect pests can be reduced, and the lodging resistance of the sugarcane is improved. The leaf can be peeled off mechanically or manually.
The remaining steps not mentioned can be processed by the existing method.
Treatment group 2: respectively applying 5 kg/mu of nitrogen fertilizer in the seedling stage and the elongation stage, and applying N to P2O5:K2The phosphorus-potassium fertilizer is applied according to the proportion of 2:1:2, and the rest methods are the same as the treatment group 1.
Treatment group 3: applying nitrogen fertilizer 5 kg/mu respectively at planting time and 3-leaf stage, and applying N: P2O5:K2The phosphorus-potassium fertilizer is applied according to the proportion of 2:1:2, and the rest methods are the same as the treatment group 1.
Treatment group 4: the soil pH value is not adjusted when the soil is adjusted, no micro-fertilizer is applied, and the other methods are the same as the treatment group 1.
Treatment group 5: the water holding capacity of the soil surface layer is not monitored in the whole growth period, artificial watering is not carried out in the current year of planting in the case of long-term drought weather, and other methods are the same as the treatment group 1.
Treatment group 6: applying nitrogen fertilizer according to 15 kg/mu when the sugarcane grows to 3-4 true leaves, and simultaneously, applying nitrogen fertilizer according to N: P2O5:K2And (3) applying a phosphorus-potassium fertilizer and a micro-fertilizer simultaneously in a ratio of 4:2:3, wherein the micro-fertilizer is a micro-fertilizer chelating trace elements such as iron, zinc and the like. The rest of the method was the same as treatment group 1.
Treatment group 7: and deeply applying 10 kg/mu of microbial organic fertilizer during planting, covering soil, then planting the sugarcane, and planting and managing the sugarcane according to the conventional method.
Before the sugarcane is harvested, 3 points of the sugarcane are respectively selected for the 7 treatments, the sugar content is measured, the average value is obtained, and meanwhile the acre yield of each treatment is counted, and the specific table is shown in table 2.
Table 2 yield and sugar performance of different treated sugarcane:
as can be seen from Table 2, the nitrogen fertilizer is applied once when the sugarcane grows to 3-4 true leaves, the effect is better than that of the application in distribution times, and meanwhile, the nitrogen fixation of the sugarcane is related to the pH adjustment of soil, the water supply, the trace element supply and the like, so that a good environment is provided for the biological nitrogen fixation of the sugarcane in the early stage, the root growth of the sugarcane is promoted, and the biological nitrogen fixation of the sugarcane is facilitated.
In conclusion, the invention applies nitrogen fertilizer once in the early growth stage (3-4 true leaves) of sugarcane, and the nitrogen application amount is 10-15kg/hm2The method can accelerate the early growth of the sugarcane, so that the sugarcane plants form strong root systems and strong overground stems and leaves, the sugarcane is closed as early as possible, and the fertilizer is not applied any more in a large growth period (elongation period) after the sugarcane is closed, so that the biological nitrogen fixation of the sugarcane is promoted, and the nitrogen nutrition required by the growth of the sugarcane is provided. The method combines the application of nitrogen fertilizer with the adjustment of soil pH, the supply of water, the supply of phosphorus potassium fertilizer and trace elements, provides a good environment for biological nitrogen fixation of the sugarcane, and is matched with field management measures such as insect prevention, weeding and the like, thereby being beneficial to the growth of the sugarcane and promoting the biological nitrogen fixation of the sugarcane.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. A method of inducing biological nitrogen fixation in sugar cane, comprising:
(1) sweet tasteWhen the sugarcane seedlings grow to 3-4 true leaves, nitrogen fertilizer is applied according to the soil fertility status by 10-15 kg/mu, and N: P is added2O5:K2Applying phosphorus-potassium fertilizer according to the proportion of O =1:0.5:1, and covering soil after applying the fertilizer;
(2) the sugarcane is not fertilized after the sugarcane is elongated, so that the moisture supply is ensured.
2. The method for inducing biological nitrogen fixation of sugarcane according to claim 1, wherein in the step (1), a proper amount of micro-fertilizer is applied simultaneously with the application of the phosphorus-potassium fertilizer.
3. The method for inducing biological nitrogen fixation of sugarcane according to claim 2, wherein in the step (1), the nitrogen fertilizer is a quick-acting nitrogen fertilizer, and the micro-fertilizer is a micro-fertilizer for chelating trace elements such as iron, zinc and molybdenum.
4. The method for inducing biological nitrogen fixation in sugarcane according to claim 2, wherein ammonium molybdate is sprayed on the leaf surfaces if the micro-fertilizer does not contain molybdenum.
5. The method for inducing biological nitrogen fixation in sugarcane according to claim 1, wherein the pH value of the soil is adjusted to 6.8-7.5 before the sugarcane is planted.
6. The method for inducing biological nitrogen fixation of sugarcane according to claim 1, wherein before sugarcane planting, deep ploughing and deep scarification are carried out on a planting land, and soil is covered for 5-6cm after fertilization in a true leaf period.
7. The method for inducing biological nitrogen fixation of sugarcane according to claim 1, wherein in the step (2), the field management in the sugarcane seedling stage ensures that a proper amount of soil moisture is kept, namely, 60-80% of the field moisture capacity of the soil is kept within 30cm of the surface layer of the soil in the seedling stage.
8. The method for inducing biological nitrogen fixation of sugarcane according to claim 1, wherein sugarcane seed stems are soaked with 10% clothianidin and 40% bromacyl-thiamethoxam suspension seed coating agent for 8-10min before sugarcane planting.
9. The method for inducing biological nitrogen fixation of sugar cane according to claim 1, wherein the sugar cane is planted with a mulching film and a herbicide selected from at least one of atrazine, acetochlor or diuron is applied.
10. The method for inducing biological nitrogen fixation of sugarcane according to claim 1, wherein the sugarcane variety is any one of cassia B9, cassia sugar No. 29, cassia sugar No. 42, cassia sugar No. 44, cassia sugar No. 46, cassia sugar No. 49, cassia sugar No. 55, cassia sugar No. 58, cassia sugar No. 60 and cassia willow 05-136.
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| CN103283472A (en) * | 2013-06-20 | 2013-09-11 | 广西壮族自治区农业科学院甘蔗研究所 | Method for controlling soil moisture and cultivating developed root systems of black skin sugar canes |
| CN111567350A (en) * | 2020-04-30 | 2020-08-25 | 广西壮族自治区农业科学院 | Planting method for promoting nitrogen fixation of sugarcane |
| CN112219669A (en) * | 2020-09-02 | 2021-01-15 | 广西南亚热带农业科学研究所 | Simple cultivation method for sugarcane seedlings |
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| CN103283472A (en) * | 2013-06-20 | 2013-09-11 | 广西壮族自治区农业科学院甘蔗研究所 | Method for controlling soil moisture and cultivating developed root systems of black skin sugar canes |
| CN111567350A (en) * | 2020-04-30 | 2020-08-25 | 广西壮族自治区农业科学院 | Planting method for promoting nitrogen fixation of sugarcane |
| CN112219669A (en) * | 2020-09-02 | 2021-01-15 | 广西南亚热带农业科学研究所 | Simple cultivation method for sugarcane seedlings |
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