CN111534504B - 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 - Google Patents
将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 Download PDFInfo
- Publication number
- CN111534504B CN111534504B CN202010095234.4A CN202010095234A CN111534504B CN 111534504 B CN111534504 B CN 111534504B CN 202010095234 A CN202010095234 A CN 202010095234A CN 111534504 B CN111534504 B CN 111534504B
- Authority
- CN
- China
- Prior art keywords
- polynucleotide
- leu
- gly
- ala
- val
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
- C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
- C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010095234.4A CN111534504B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBPCT/GB2014/050175 | 2014-01-22 | ||
| PCT/GB2014/050175 WO2014135838A1 (en) | 2013-03-08 | 2014-01-22 | Enzyme stalling method |
| GB1406151.9 | 2014-04-04 | ||
| GBGB1406151.9A GB201406151D0 (en) | 2014-04-04 | 2014-04-04 | Method |
| GBGB1406155.0A GB201406155D0 (en) | 2014-04-04 | 2014-04-04 | Method |
| GB1406155.0 | 2014-04-04 | ||
| GB1416197.0 | 2014-09-12 | ||
| GB201416197A GB201416197D0 (en) | 2014-09-12 | 2014-09-12 | Method |
| CN202010095234.4A CN111534504B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| CN201580005650.8A CN106103741B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| PCT/GB2015/050140 WO2015110813A1 (en) | 2014-01-22 | 2015-01-22 | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580005650.8A Division CN106103741B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111534504A CN111534504A (zh) | 2020-08-14 |
| CN111534504B true CN111534504B (zh) | 2024-06-21 |
Family
ID=53680879
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010095234.4A Active CN111534504B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| CN201580005650.8A Active CN106103741B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580005650.8A Active CN106103741B (zh) | 2014-01-22 | 2015-01-22 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US10385389B2 (https=) |
| EP (1) | EP3097210B2 (https=) |
| JP (1) | JP6749243B2 (https=) |
| CN (2) | CN111534504B (https=) |
| AU (1) | AU2015208919B9 (https=) |
| CA (1) | CA2937411C (https=) |
| WO (1) | WO2015110813A1 (https=) |
Families Citing this family (77)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| CA2959220A1 (en) | 2014-09-01 | 2016-03-10 | Vib Vzw | Mutant csgg pores |
| EP3204511B1 (en) | 2014-10-07 | 2021-07-28 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| WO2016166232A1 (en) | 2015-04-14 | 2016-10-20 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
| CN116200476A (zh) | 2016-03-02 | 2023-06-02 | 牛津纳米孔科技公开有限公司 | 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔 |
| EP4397970A3 (en) | 2016-04-06 | 2024-10-09 | Oxford Nanopore Technologies PLC | Mutant pore |
| CN109196116B (zh) | 2016-05-25 | 2022-09-27 | 牛津纳米孔科技公开有限公司 | 一种表征靶多核苷酸的方法 |
| GB201609220D0 (en) * | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB2559117B (en) | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| CA3053086A1 (en) * | 2017-02-10 | 2018-08-16 | Oxford Nanopore Technologies Limited | Modified nanopores, compositions comprising the same, and uses thereof |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN117106038B (zh) | 2017-06-30 | 2025-12-09 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB2569977A (en) * | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| GB201801768D0 (en) * | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB2574197B (en) | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB201811811D0 (en) | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| JP6545888B1 (ja) * | 2018-11-09 | 2019-07-17 | 国立大学法人東北大学 | 情報処理システム、公開鍵の変更方法、およびプログラム |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| WO2021053745A1 (ja) * | 2019-09-18 | 2021-03-25 | 株式会社日立ハイテク | アダプター分子、当該アダプター分子と生体分子とが結合した生体分子－アダプター分子複合体、生体分子分析装置及び生体分子分析方法 |
| WO2021053744A1 (ja) * | 2019-09-18 | 2021-03-25 | 株式会社日立ハイテク | アダプター分子、当該アダプター分子と生体分子とが結合した生体分子－アダプター分子複合体、生体分子分析装置及び生体分子分析方法 |
| CN111164097B (zh) | 2019-09-29 | 2021-02-19 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| WO2021056598A1 (zh) | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mmup单体变体及其应用 |
| WO2021056599A1 (zh) * | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| US20230295712A1 (en) * | 2020-06-18 | 2023-09-21 | Oxford Nanopore Technologies Plc | A method of selectively characterising a polynucleotide using a detector |
| JP2023530155A (ja) * | 2020-06-18 | 2023-07-13 | オックスフォード ナノポール テクノロジーズ ピーエルシー | ナノポアを通って移動するポリヌクレオチドを特徴付ける方法 |
| EP4193363A1 (en) | 2020-08-07 | 2023-06-14 | Oxford Nanopore Technologies plc | Methods of identifying nucleic acid barcodes |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| CN112851771A (zh) * | 2021-02-09 | 2021-05-28 | 中国科学院武汉病毒研究所 | 连接核酸与蛋白或肽的方法 |
| CN112851765B (zh) * | 2021-02-09 | 2023-02-28 | 中国科学院武汉病毒研究所 | 蛋白或肽与核酸共价连接的方法 |
| EP4341433A1 (en) | 2021-05-19 | 2024-03-27 | Oxford Nanopore Technologies PLC | Methods for complement strand sequencing |
| CN114134142B (zh) * | 2021-11-23 | 2024-05-28 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN113862264A (zh) * | 2021-12-03 | 2021-12-31 | 北京齐碳科技有限公司 | 用于靶多核苷酸测序的衔接子、构建体、方法和应用 |
| CN114262735B (zh) * | 2021-12-15 | 2025-09-16 | 成都齐碳科技有限公司 | 用于表征多核苷酸的衔接体及其用途 |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202204919D0 (en) | 2022-04-04 | 2022-05-18 | Oxford Nanopore Tech Plc | Method |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| CN114854826A (zh) * | 2022-05-13 | 2022-08-05 | 北京齐碳科技有限公司 | 序列、包含序列的接头及其用途 |
| JP2025517736A (ja) | 2022-05-17 | 2025-06-10 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 方法及びアダプター |
| CN119095960A (zh) | 2022-05-18 | 2024-12-06 | 牛津纳米孔科技公开有限公司 | 用于核酸提取和纯化的组合物和方法 |
| GB202207267D0 (en) | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
| CN114836452B (zh) * | 2022-05-19 | 2023-12-05 | 浙江安诺优达生物科技有限公司 | 一种密码子优化的核酸外切酶ⅲ基因及其表达方法 |
| CN116478983B (zh) * | 2022-11-21 | 2023-10-24 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| CN120344725A (zh) * | 2022-12-26 | 2025-07-18 | 深圳华大生命科学研究院 | 减少测序文库中游离接头的方法 |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202307494D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Ltd | Nanopore sequencing and preparation modular instrument |
| GB202315935D0 (en) | 2023-10-18 | 2023-11-29 | Oxford Nanopore Tech Ltd | Novel enzymes |
| GB202407247D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
| WO2025241113A1 (zh) * | 2024-05-22 | 2025-11-27 | 深圳华大生命科学研究院 | 控制多核苷酸移动的方法及解旋酶变体 |
| WO2026057755A1 (en) | 2024-09-13 | 2026-03-19 | Oxford Nanopore Technologies Plc | Adaptors and kits for rna molecules labelling and characterising |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013014451A1 (en) * | 2011-07-25 | 2013-01-31 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
Family Cites Families (115)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| JP3891620B2 (ja) | 1996-11-14 | 2007-03-14 | 株式会社アイシン・コスモス研究所 | ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法 |
| US20020197614A1 (en) * | 1997-05-16 | 2002-12-26 | Mosaic Technologies, Inc. | Electrophoretic analysis of target molecules using adapter molecules |
| AU8586298A (en) | 1997-07-25 | 1999-02-16 | University Of Massachusetts | Designed protein pores as components for biosensors |
| US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
| US6150112A (en) * | 1998-09-18 | 2000-11-21 | Yale University | Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
| NO986133D0 (no) | 1998-12-23 | 1998-12-23 | Preben Lexow | FremgangsmÕte for DNA-sekvensering |
| EP2383776B1 (en) | 1999-06-22 | 2015-02-25 | President and Fellows of Harvard College | Solid state nanopore device for evaluating biopolymers |
| EP1208207A2 (de) | 1999-08-31 | 2002-05-29 | Michael Niederweis | Verfahren zur herstellung eines kanalbildenden proteins |
| WO2001059453A2 (en) | 2000-02-11 | 2001-08-16 | The Texas A & M University System | Biosensor compositions and methods of use |
| WO2001062943A1 (en) * | 2000-02-25 | 2001-08-30 | Invitrogen Corporation | Topoisomerase linker-mediated amplification methods |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| WO2002042496A2 (en) | 2000-11-27 | 2002-05-30 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US6863833B1 (en) | 2001-06-29 | 2005-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Microfabricated apertures for supporting bilayer lipid membranes |
| EP1504114B1 (en) | 2002-05-10 | 2017-07-12 | The Texas A & M University System | Stochastic sensing through covalent interactions |
| US20040209299A1 (en) * | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| AU2004225520A1 (en) * | 2003-03-25 | 2004-10-14 | Stratagene | DNA polymerase fusions and uses thereof |
| US7163658B2 (en) | 2003-04-23 | 2007-01-16 | Rouvain Bension | Rapid sequencing of polymers |
| AU2003904237A0 (en) | 2003-08-08 | 2003-08-21 | Garvan Institute Of Medical Research | Novel translocation assay |
| US7424370B2 (en) | 2004-02-06 | 2008-09-09 | Council Of Scientific And Industrial Research | Computational method for identifying adhesin and adhesin-like proteins of therapeutic potential |
| JP2005253427A (ja) * | 2004-03-15 | 2005-09-22 | Aisin Seiki Co Ltd | 核酸検出方法及び核酸単離方法 |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| US20060105461A1 (en) | 2004-10-22 | 2006-05-18 | May Tom-Moy | Nanopore analysis system |
| EP1842061A4 (en) | 2004-12-21 | 2009-05-13 | Texas A & M Univ Sys | HIGH-TEMPERATURE ION CHANNELS AND ION PORES |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| KR100730350B1 (ko) | 2005-10-17 | 2007-06-19 | 삼성전자주식회사 | 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치 |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| WO2007075987A2 (en) | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Active surface coupled polymerases |
| US7849581B2 (en) | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| US7638034B2 (en) | 2006-09-21 | 2009-12-29 | Los Alamos National Security, Llc | Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions |
| US20110121840A1 (en) | 2007-02-20 | 2011-05-26 | Gurdial Singh Sanghera | Lipid Bilayer Sensor System |
| EP2156179B1 (en) | 2007-04-04 | 2021-08-18 | The Regents of The University of California | Methods for using a nanopore |
| WO2009020682A2 (en) | 2007-05-08 | 2009-02-12 | The Trustees Of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| GB0716264D0 (en) | 2007-08-21 | 2007-09-26 | Isis Innovation | Bilayers |
| WO2009035647A1 (en) | 2007-09-12 | 2009-03-19 | President And Fellows Of Harvard College | High-resolution molecular graphene sensor comprising an aperture in the graphene layer |
| GB2453377A (en) | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
| US8951731B2 (en) † | 2007-10-15 | 2015-02-10 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8231969B2 (en) | 2008-03-26 | 2012-07-31 | University Of Utah Research Foundation | Asymmetrically functionalized nanoparticles |
| EP4230747A3 (en) | 2008-03-28 | 2023-11-15 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
| WO2009132124A2 (en) | 2008-04-24 | 2009-10-29 | The Trustees Of Columbia University In The City Of New York | Geometric patterns and lipid bilayers for dna molecule organization and uses thereof |
| EP2293921A4 (en) | 2008-05-22 | 2013-05-22 | Univ California | MEMBRANE PROBLEMS AND MEMBRANES MADE FROM THESE |
| US8652771B2 (en) | 2008-05-28 | 2014-02-18 | University of Souther California | Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects |
| US20110229877A1 (en) | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| EP2310534B1 (en) | 2008-07-07 | 2018-09-05 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| US20100092960A1 (en) | 2008-07-25 | 2010-04-15 | Pacific Biosciences Of California, Inc. | Helicase-assisted sequencing with molecular beacons |
| CA2774710C (en) | 2008-09-22 | 2016-08-02 | University Of Washington | Msp nanopores and related methods |
| US8383369B2 (en) | 2008-09-24 | 2013-02-26 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| KR20110125226A (ko) | 2009-01-30 | 2011-11-18 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 혼성화 링커 |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| CN102405410B (zh) | 2009-04-20 | 2014-06-25 | 牛津楠路珀尔科技有限公司 | 脂质双层传感器阵列 |
| US9127313B2 (en) | 2009-12-01 | 2015-09-08 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
| CN102834716B (zh) | 2010-02-23 | 2016-03-30 | 华盛顿大学 | 人工分枝菌酸膜 |
| WO2011125015A2 (en) | 2010-04-05 | 2011-10-13 | Bar-Ilan University | Protease-activatable pore-forming polypeptides |
| US8828211B2 (en) | 2010-06-08 | 2014-09-09 | President And Fellows Of Harvard College | Nanopore device with graphene supported artificial lipid membrane |
| WO2012042226A2 (en) | 2010-10-01 | 2012-04-05 | Oxford Nanopore Technologies Limited | Biochemical analysis apparatus and rotary valve |
| CN102116783B (zh) | 2010-12-31 | 2013-05-29 | 北京普源精电科技有限公司 | 一种波形显示方法 |
| CN102174554A (zh) | 2011-01-24 | 2011-09-07 | 内蒙古民族大学 | 双控双调节原核表达载体系统及其构建方法和用途 |
| US20120196279A1 (en) | 2011-02-02 | 2012-08-02 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
| KR101939420B1 (ko) | 2011-02-11 | 2019-01-16 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 세공 |
| US8980553B2 (en) * | 2011-04-02 | 2015-03-17 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
| EP4737389A2 (en) | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| WO2012166906A1 (en) | 2011-05-31 | 2012-12-06 | Massachusetts Institute Of Technology | Cell-directed synthesis of multifunctional nanopatterns and nanomaterials |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| CN104039979B (zh) † | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| GB201120910D0 (en) | 2011-12-06 | 2012-01-18 | Cambridge Entpr Ltd | Nanopore functionality control |
| US9617591B2 (en) † | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| AU2012360244B2 (en) * | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| CN107828877A (zh) * | 2012-01-20 | 2018-03-23 | 吉尼亚科技公司 | 基于纳米孔的分子检测与测序 |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| TWI655213B (zh) | 2012-07-13 | 2019-04-01 | Menicon Co., Ltd. | 自我組織化肽衍生物的製造方法 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2879261C (en) † | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6429773B2 (ja) † | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| JP6375301B2 (ja) | 2012-10-26 | 2018-08-15 | オックスフォード ナノポール テクノロジーズ リミテッド | 液滴界面 |
| US9683230B2 (en) † | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| US10179933B2 (en) | 2013-02-07 | 2019-01-15 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Hybrid nanopores and uses thereof for detection of analytes |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| DK2970951T3 (da) † | 2013-03-13 | 2019-05-13 | Illumina Inc | Fremgangsmåder til nukleinsyresekventering |
| US10613076B2 (en) | 2013-03-14 | 2020-04-07 | The Trustees Of Boston University | Optoelectronic control of solid-state nanopores |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| AU2014270410B2 (en) | 2013-05-24 | 2020-07-16 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing |
| US20150011398A1 (en) * | 2013-06-17 | 2015-01-08 | Kim Lewis | Methods for quantitative determination of protein-nucleic acid interactions in complex mixtures |
| WO2015051378A1 (en) | 2013-10-04 | 2015-04-09 | University Of Washington Through Its Center For Commercialization | Systems and methods for nanopore-based analysis of nucleic acids |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| SI3074534T1 (sl) | 2013-11-26 | 2019-08-30 | Illumina, Inc. | Postopki določanja zaporedja polinukleotidov |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| WO2015150786A1 (en) | 2014-04-04 | 2015-10-08 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| FR3023394B1 (fr) | 2014-07-02 | 2017-12-29 | Adn Access Data Networks | Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture |
| CA2959220A1 (en) | 2014-09-01 | 2016-03-10 | Vib Vzw | Mutant csgg pores |
| EP3204511B1 (en) | 2014-10-07 | 2021-07-28 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| WO2016166232A1 (en) | 2015-04-14 | 2016-10-20 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
| CN116200476A (zh) | 2016-03-02 | 2023-06-02 | 牛津纳米孔科技公开有限公司 | 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔 |
| EP4397970A3 (en) | 2016-04-06 | 2024-10-09 | Oxford Nanopore Technologies PLC | Mutant pore |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN117106038B (zh) | 2017-06-30 | 2025-12-09 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| WO2021092433A2 (en) * | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
-
2015
- 2015-01-22 CN CN202010095234.4A patent/CN111534504B/zh active Active
- 2015-01-22 EP EP15704360.5A patent/EP3097210B2/en active Active
- 2015-01-22 WO PCT/GB2015/050140 patent/WO2015110813A1/en not_active Ceased
- 2015-01-22 CN CN201580005650.8A patent/CN106103741B/zh active Active
- 2015-01-22 CA CA2937411A patent/CA2937411C/en active Active
- 2015-01-22 US US15/113,207 patent/US10385389B2/en active Active
- 2015-01-22 AU AU2015208919A patent/AU2015208919B9/en active Active
- 2015-01-22 JP JP2016547868A patent/JP6749243B2/ja active Active
-
2019
- 2019-08-14 US US16/540,425 patent/US11725235B2/en active Active
-
2023
- 2023-06-23 US US18/340,823 patent/US20240026441A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013014451A1 (en) * | 2011-07-25 | 2013-01-31 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2937411C (en) | 2023-09-26 |
| US10385389B2 (en) | 2019-08-20 |
| EP3097210B2 (en) | 2022-04-20 |
| US20240026441A1 (en) | 2024-01-25 |
| AU2015208919A1 (en) | 2016-07-21 |
| US20170107569A1 (en) | 2017-04-20 |
| US11725235B2 (en) | 2023-08-15 |
| CA2937411A1 (en) | 2015-07-30 |
| EP3097210B1 (en) | 2018-11-14 |
| JP2017503517A (ja) | 2017-02-02 |
| US20200087724A1 (en) | 2020-03-19 |
| CN111534504A (zh) | 2020-08-14 |
| CN106103741A (zh) | 2016-11-09 |
| CN106103741B (zh) | 2020-03-13 |
| EP3097210A1 (en) | 2016-11-30 |
| JP6749243B2 (ja) | 2020-09-02 |
| AU2015208919B2 (en) | 2021-03-11 |
| AU2015208919B9 (en) | 2021-04-01 |
| WO2015110813A1 (en) | 2015-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111534504B (zh) | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 | |
| US11685949B2 (en) | Mutant pore | |
| CA2937287C (en) | Method for controlling the movement of a polynucleotide through a transmembrane pore | |
| EP3619224B1 (en) | Transmembrane pore consisting of two csgg pores | |
| CN107002151B (zh) | 向跨膜孔输送分析物的方法 | |
| CN106460061B (zh) | 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法 | |
| EP3126516B1 (en) | Method of target molecule characterisation using a molecular pore |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: oxford Applicant after: Oxford nanopore Technology Public Co.,Ltd. Address before: oxford Applicant before: OXFORD NANOPORE TECHNOLOGIES LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |